Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr3

Orthogroup ID

OG0016363

Physical Location & Seq

Genomic Coordinates Forward (+)

9758419 .. 9761557

Gene Structure 3139 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw3G010180.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1293 bp

ATGGATTCGAACAAAGAACACCTCTTGGATAATGGCAACTTGAAGAGAACGAGTAGTAACGAGCAGGAGGTTCACCATGGCATGACTGCTAGACAATTGTCATCTTCTTCGCTGCAAAACAAGTCGGTTCCTACACTACTACCTATTAGTGTGCAAAATGGGAAGGTTAGGAAGGTACTCATCAATTTTCAGGTTGTTCTTTTGGGGACCAAGCGTTCGGTACTCCTTCCCACCATTCCTCTATCCTTGGTTGCTGTCTACCGTGGTATTGGAAAGGTCATAGCATACTACACTGTGGGAGGGCTTCTAAATGCAACATTTGGAAATGCCAGAGAGCTAATTATCGCAATTTCAGCTCTAAGTCAAAACAAATTGCGATTGCTCAAGTACTCTCTCTTGGGTTCAGTTTTTTCAAATCTTCTTTTAGTCCTCGGGACATCACTTTTCTGTGGTGGCCTTGCCAACCTAAGAAGTGAGCAAAAATTCGAAAGAAGGCAGGCCCAAGTGAACTCGCTCCTTCTAATGCTAGGTCCATTCTGTCATATGCTGCCTATGTTGCTTCAATATGCCGGGGACTCCGCTGGTAAAGAAGGCCAAATACTCGAGCTATCAAGGGCCCTTAGTATCATCATGCTGCTTTCATATGCTACATACCTGTTCTTCCAGTTATGGACTCACCGCACATTTTTCGAATCACTCGAAGAATCAAATGATGATGAGGATGATGATGTGGCTGTAGTAGGTTTCTGGAGTGGATTTGTTTGGTTATTTGTTGTTACAATCACCACAGCTTGGTTGTCTGAGTATGTAGTTGGCACAATTGAGGTAATTCCCACTGTTTTATATAATGTCTTCCTCAGCCTCATCTTGGTTCCAATTCTTGGAAATGCAGCAGAGCATGCAGGTTCAATCATATTTGTTCTTAAAAACAAGTTGGATATATCTTTAGGAGTCTCTTTGGGATCTGCAACTCAGATCGCTATGTTCGTGGTGCCATCGAGCGTTGTTGTTGCTTGGATAATGGGCATTAACATGGATATTAACTTCAATCTCATCGAGACGACGTCTCTTGCTCTGGCAGTGATCACCACATCAATTGCTCTACAGGTAATCTTCACATTCATAGTACATACATCCATAACTTCCTATATATTGGAGTCTTCTAAATCAAATTCCTTTTGGTTGGTTCTTATTCTCTGCTACATTGTTATTGGGATCTGCTTCTTTATATTCGAAACACCAATGGGTAAGACGAACATGCACTGGAGACTGAAACCATGGATGAAAGAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006793 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0006873 GO:0006874 GO:0006875 GO:0006882 GO:0008150 GO:0008152 GO:0008324 GO:0009605 GO:0009607 GO:0009624 GO:0009705 GO:0009987 GO:0010035 GO:0010038 GO:0015075 GO:0015077 GO:0015078 GO:0015085 GO:0015291 GO:0015297 GO:0015298 GO:0015299 GO:0015318 GO:0015368 GO:0015369 GO:0015491 GO:0015672 GO:0016020 GO:0019725 GO:0022804 GO:0022857 GO:0022890 GO:0030001 GO:0030003 GO:0030026 GO:0031090 GO:0034220 GO:0042221 GO:0042592 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046916 GO:0048878 GO:0050801 GO:0050896 GO:0051139 GO:0051179 GO:0051234 GO:0051592 GO:0051704 GO:0051707 GO:0055062 GO:0055065 GO:0055069 GO:0055071 GO:0055074 GO:0055076 GO:0055080 GO:0055081 GO:0055082 GO:0055083 GO:0055085 GO:0065007 GO:0065008 GO:0070588 GO:0070838 GO:0072503 GO:0072505 GO:0072506 GO:0072507 GO:0072511 GO:0098588 GO:0098655 GO:0098660 GO:0098662 GO:0098771 GO:0098805 GO:0099516 GO:1902600

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Na_Ca_ex

Protein Analysis

430

Amino Acids

47.67

Weight (kDa)

7.7

Isoelectric Point (pI)

34.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Na_Ca_ex	PF01699	94 - 224	1.7e-14	Sodium/calcium exchanger protein
Na_Ca_ex	PF01699	252 - 397	5.2e-09	Sodium/calcium exchanger protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016363)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g11490
malus_domestica	MD04G1151900.v1.1
prunus_persica	Prupe.6G277000_v2.0.a1
pyrus_communis	pycom04g13790
rosa_chinensis	RchiOBHm_Chr3g0463181
rosa_laevigata	RLG00000024805
rosa_roxburghii	Rroxscaffold_6G00416850 Rroxscaffold_6G00416870
rosa_rugosa	Rorug03G0062100.1
rosa_samantha	Rh3AG120300 Rh3BG124700 Rh3CG126700 Rh3DG125700
rosa_wichuraiana	Rw3G010180 Rw3G010210

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	1067
Acc36I	ACCTGC	1 cut(s)	893
AccB1I	GGYRCC	1 cut(s)	991
AccB7I	CCANNNNNTGG	2 cut(s)	792, 881
AccI	GTMKAC	1 cut(s)	258
AciI	CCGC	2 cut(s)	579, 679
AclWI	GGATC	2 cut(s)	970, 1223
AcsI	RAATTY	2 cut(s)	482, 1171
AcyI	GRCGYC	1 cut(s)	1064
AfaI	GTAC	4 cut(s)	177, 222, 389, 1128
AfiI	CCNNNNNNNGG	3 cut(s)	792, 868, 881
AgsI	TTSAA	5 cut(s)	43, 414, 563, 909, 1048
AloI	GAACNNNNNNTCC	2 cut(s)	199, 231
AluBI	AGCT	4 cut(s)	337, 356, 607, 791
AluI	AGCT	4 cut(s)	337, 356, 607, 791
Alw26I	GTCTC	4 cut(s)	958, 1052, 1071, 1261
AlwI	GGATC	2 cut(s)	970, 1223
Ama87I	CYCGRG	2 cut(s)	431, 602
AoxI	GGCC	4 cut(s)	454, 498, 592, 615
ApaI	GGGCCC	1 cut(s)	619
ApeKI	GCWGC	4 cut(s)	112, 547, 634, 890
ApoI	RAATTY	2 cut(s)	482, 1171
ArsI	GACNNNNNNTTYG	2 cut(s)	199, 231
AspS9I	GGNCC	5 cut(s)	207, 499, 530, 615, 616
AsuC2I	CCSGG	1 cut(s)	571
AsuHPI	GGTGA	4 cut(s)	65, 668, 775, 1078
AsuII	TTCGAA	4 cut(s)	8, 486, 690, 1233
AvaI	CYCGRG	2 cut(s)	431, 602
AvaII	GGWCC	2 cut(s)	207, 530
BaeGI	GKGCMC	1 cut(s)	619
BanI	GGYRCC	1 cut(s)	991
BanII	GRGCYC	1 cut(s)	619
BarI	GAAGNNNNNNTAC	2 cut(s)	582, 614
BbsI	GAAGAC	2 cut(s)	844, 1152
BbvCI	CCTCAGC	1 cut(s)	857
BbvI	GCAGC	4 cut(s)	99, 534, 621, 902
BccI	CCATC	1 cut(s)	1003
BcgI	CGANNNNNNTGC	2 cut(s)	670, 704
BclI	TGATCA	1 cut(s)	1083
BcnI	CCSGG	1 cut(s)	571
BcoDI	GTCTC	4 cut(s)	958, 1052, 1071, 1261
BfaI	CTAG	2 cut(s)	90, 527
BfmI	CTRYAG	2 cut(s)	735, 1103
BfuAI	ACCTGC	1 cut(s)	893
BisI	GCNGC	4 cut(s)	113, 548, 635, 891
BlsI	GCNGC	4 cut(s)	114, 549, 636, 892
BmcAI	AGTACT	1 cut(s)	389
Bme1390I	CCNGG	1 cut(s)	571
Bme18I	GGWCC	2 cut(s)	207, 530
BmeT110I	CYCGRG	2 cut(s)	431, 602
BmgT120I	GGNCC	5 cut(s)	207, 499, 530, 615, 616
BmiI	GGNNCC	5 cut(s)	129, 208, 617, 873, 993
BmrFI	CCNGG	1 cut(s)	571
BoxI	GACNNNNGTC	1 cut(s)	97
BpiI	GAAGAC	2 cut(s)	844, 1152
BpmI	CTGGAG	2 cut(s)	769, 1285
Bpu10I	CCTNAGC	1 cut(s)	857
Bpu14I	TTCGAA	4 cut(s)	8, 486, 690, 1233
BpuEI	CTTGAG	1 cut(s)	368
BpuMI	CCSGG	1 cut(s)	571
BsaHI	GRCGYC	1 cut(s)	1064
BsaJI	CCNNGG	6 cut(s)	76, 246, 262, 430, 570, 1277
Bsc4I	CCNNNNNNNGG	3 cut(s)	792, 868, 881
Bse1I	ACTGG	2 cut(s)	664, 1268
BseDI	CCNNGG	6 cut(s)	76, 246, 262, 430, 570, 1277
BseGI	GGATG	3 cut(s)	727, 1133, 1287
BseLI	CCNNNNNNNGG	3 cut(s)	792, 868, 881
BseMII	CTCAG	3 cut(s)	792, 871, 986
BseNI	ACTGG	2 cut(s)	664, 1268
BseSI	GKGCMC	1 cut(s)	619
BseXI	GCAGC	4 cut(s)	99, 534, 621, 902
BshFI	GGCC	4 cut(s)	456, 500, 594, 617
BshNI	GGYRCC	1 cut(s)	991
BsiHKCI	CYCGRG	2 cut(s)	431, 602
BsiSI	CCGG	1 cut(s)	570
BslFI	GGGAC	3 cut(s)	220, 448, 587
BslI	CCNNNNNNNGG	3 cut(s)	792, 868, 881
BsmAI	GTCTC	4 cut(s)	958, 1052, 1071, 1261
BsmBI	CGTCTC	2 cut(s)	1052, 1071
BsmFI	GGGAC	3 cut(s)	220, 448, 587
BsnI	GGCC	4 cut(s)	456, 500, 594, 617
BsoBI	CYCGRG	2 cut(s)	431, 602
Bsp119I	TTCGAA	4 cut(s)	8, 486, 690, 1233
Bsp120I	GGGCCC	1 cut(s)	615
Bsp1286I	GDGCHC	1 cut(s)	619
Bsp143I	GATC	4 cut(s)	962, 975, 1083, 1215
Bsp19I	CCATGG	2 cut(s)	76, 1277
BspACI	CCGC	2 cut(s)	579, 679
BspANI	GGCC	4 cut(s)	456, 500, 594, 617
BspCNI	CTCAG	3 cut(s)	793, 870, 985
BspLI	GGNNCC	5 cut(s)	129, 208, 617, 873, 993
BspMI	ACCTGC	1 cut(s)	893
BspPI	GGATC	2 cut(s)	970, 1223
BspT104I	TTCGAA	4 cut(s)	8, 486, 690, 1233
BspT107I	GGYRCC	1 cut(s)	991
BsrI	ACTGG	2 cut(s)	664, 1268
BssECI	CCNNGG	6 cut(s)	76, 246, 262, 430, 570, 1277
BssMI	GATC	4 cut(s)	962, 975, 1083, 1215
BssNI	GRCGYC	1 cut(s)	1064
BssT1I	CCWWGG	3 cut(s)	76, 246, 1277
Bst4CI	ACNGT	3 cut(s)	263, 295, 838
Bst6I	CTCTTC	1 cut(s)	38
BstACI	GRCGYC	1 cut(s)	1064
BstAPI	GCANNNNNTGC	1 cut(s)	899
BstBI	TTCGAA	4 cut(s)	8, 486, 690, 1233
BstC8I	GCNNGC	2 cut(s)	498, 900
BstDEI	CTNAG	6 cut(s)	359, 467, 620, 801, 857, 972
BstDSI	CCRYGG	3 cut(s)	76, 262, 1277
BstF5I	GGATG	3 cut(s)	727, 1133, 1287
BstKTI	GATC	4 cut(s)	965, 978, 1086, 1218
BstMAI	GTCTC	4 cut(s)	958, 1052, 1071, 1261
BstMBI	GATC	4 cut(s)	962, 975, 1083, 1215
BstMWI	GCNNNNNNNGC	3 cut(s)	353, 556, 899
BstNSI	RCATGY	2 cut(s)	902, 1261
BstPAI	GACNNNNGTC	1 cut(s)	97
BstSCI	CCNGG	1 cut(s)	569
BstSFI	CTRYAG	2 cut(s)	735, 1103
BstSLI	GKGCMC	1 cut(s)	619
BstV1I	GCAGC	4 cut(s)	99, 534, 621, 902
BstV2I	GAAGAC	2 cut(s)	844, 1152
BstX2I	RGATCY	2 cut(s)	962, 1215
BstYI	RGATCY	2 cut(s)	962, 1215
BsuRI	GGCC	4 cut(s)	456, 500, 594, 617
BtgI	CCRYGG	3 cut(s)	76, 262, 1277
BtsCI	GGATG	3 cut(s)	727, 1133, 1287
BtsI	GCAGTG	1 cut(s)	1086
BtsIMutI	CAGTG	4 cut(s)	291, 834, 1086, 1261
BveI	ACCTGC	1 cut(s)	893
Cac8I	GCNNGC	2 cut(s)	498, 900
Cfr13I	GGNCC	5 cut(s)	207, 499, 530, 615, 616
Csp6I	GTAC	4 cut(s)	176, 221, 388, 1127
CspCI	CAANNNNNGTGG	2 cut(s)	1078, 1113
CviAII	CATG	7 cut(s)	77, 82, 631, 899, 1033, 1258, 1278
CviQI	GTAC	4 cut(s)	176, 221, 388, 1127
DdeI	CTNAG	6 cut(s)	359, 467, 620, 801, 857, 972
DpnI	GATC	4 cut(s)	964, 977, 1085, 1217
DpnII	GATC	4 cut(s)	962, 975, 1083, 1215
Eam1104I	CTCTTC	1 cut(s)	38
EarI	CTCTTC	1 cut(s)	38
Eco130I	CCWWGG	3 cut(s)	76, 246, 1277
Eco24I	GRGCYC	1 cut(s)	619
Eco47I	GGWCC	2 cut(s)	207, 530
Eco88I	CYCGRG	2 cut(s)	431, 602
EcoO109I	RGGNCCY	2 cut(s)	615, 616
EcoT14I	CCWWGG	3 cut(s)	76, 246, 1277
EcoT38I	GRGCYC	1 cut(s)	619
ErhI	CCWWGG	3 cut(s)	76, 246, 1277
Esp3I	CGTCTC	2 cut(s)	1052, 1071
FaeI	CATG	7 cut(s)	80, 85, 634, 902, 1036, 1261, 1281
FaqI	GGGAC	3 cut(s)	220, 448, 587
FatI	CATG	7 cut(s)	76, 81, 630, 898, 1032, 1257, 1277
FauNDI	CATATG	2 cut(s)	543, 643
FbaI	TGATCA	1 cut(s)	1083
FblI	GTMKAC	1 cut(s)	258
Fnu4HI	GCNGC	4 cut(s)	113, 548, 635, 891
FokI	GGATG	2 cut(s)	734, 1120
FriOI	GRGCYC	1 cut(s)	619
Fsp4HI	GCNGC	4 cut(s)	113, 548, 635, 891
FspBI	CTAG	2 cut(s)	90, 527
GluI	GCNGC	4 cut(s)	113, 548, 635, 891
GsuI	CTGGAG	2 cut(s)	769, 1285
HaeIII	GGCC	4 cut(s)	456, 500, 594, 617
HapII	CCGG	1 cut(s)	570
Hin1I	GRCGYC	1 cut(s)	1064
Hin1II	CATG	7 cut(s)	80, 85, 634, 902, 1036, 1261, 1281
HinfI	GANTC	7 cut(s)	5, 575, 673, 692, 704, 951, 1157
HpaII	CCGG	1 cut(s)	570
HphI	GGTGA	4 cut(s)	65, 668, 775, 1078
Hpy166II	GTNNAC	3 cut(s)	73, 259, 508
Hpy188I	TCNGA	2 cut(s)	802, 975
Hpy188III	TCNNGA	3 cut(s)	433, 748, 1057
Hpy8I	GTNNAC	3 cut(s)	73, 259, 508
Hpy99I	CGWCG	1 cut(s)	1066
HpyAV	CCTTC	6 cut(s)	157, 166, 236, 486, 527, 584
HpyCH4III	ACNGT	3 cut(s)	263, 295, 838
HpyCH4IV	ACGT	1 cut(s)	1064
HpyCH4V	TGCA	7 cut(s)	115, 154, 314, 890, 902, 968, 1261
HpyF10VI	GCNNNNNNNGC	3 cut(s)	353, 556, 899
HpyF3I	CTNAG	6 cut(s)	359, 467, 620, 801, 857, 972
HpySE526I	ACGT	1 cut(s)	1064
Hsp92I	GRCGYC	1 cut(s)	1064
Hsp92II	CATG	7 cut(s)	80, 85, 634, 902, 1036, 1261, 1281
Ksp22I	TGATCA	1 cut(s)	1083
Kzo9I	GATC	4 cut(s)	962, 975, 1083, 1215
LmnI	GCTCC	1 cut(s)	519
Lsp1109I	GCAGC	4 cut(s)	99, 534, 621, 902
MaeI	CTAG	2 cut(s)	90, 527
MaeII	ACGT	1 cut(s)	1064
MaeIII	GTNAC	2 cut(s)	56, 775
MalI	GATC	4 cut(s)	964, 977, 1085, 1217
MboI	GATC	4 cut(s)	962, 975, 1083, 1215
MboII	GAAGA	9 cut(s)	55, 96, 99, 410, 652, 713, 844, 1105, 1152
MfeI	CAATTG	3 cut(s)	95, 819, 1095
MflI	RGATCY	2 cut(s)	962, 1215
MhlI	GDGCHC	1 cut(s)	619
MlyI	GAGTC	4 cut(s)	569, 667, 960, 1166
MmeI	TCCRAC	1 cut(s)	915
MnlI	CCTC	9 cut(s)	32, 61, 249, 293, 440, 712, 817, 866, 872
MseI	TTAA	3 cut(s)	924, 1029, 1041
MslI	CAYNNNNRTG	1 cut(s)	1031
MspA1I	CMGCKG	1 cut(s)	581
MspI	CCGG	1 cut(s)	570
MspR9I	CCNGG	1 cut(s)	571
MunI	CAATTG	3 cut(s)	95, 819, 1095
MwoI	GCNNNNNNNGC	3 cut(s)	353, 556, 899
NciI	CCSGG	1 cut(s)	571
NcoI	CCATGG	2 cut(s)	76, 1277
NdeI	CATATG	2 cut(s)	543, 643
NdeII	GATC	4 cut(s)	962, 975, 1083, 1215
NlaIII	CATG	7 cut(s)	80, 85, 634, 902, 1036, 1261, 1281
NlaIV	GGNNCC	5 cut(s)	129, 208, 617, 873, 993
NspI	RCATGY	2 cut(s)	902, 1261
NspV	TTCGAA	4 cut(s)	8, 486, 690, 1233
PaeI	GCATGC	1 cut(s)	902
PaeR7I	CTCGAG	1 cut(s)	602
PcsI	WCGNNNNNNNCGW	2 cut(s)	696, 984
PfeI	GAWTC	3 cut(s)	5, 692, 704
PflFI	GACNNNGTC	1 cut(s)	1063
PflMI	CCANNNNNTGG	2 cut(s)	792, 881
PkrI	GCNGC	4 cut(s)	114, 549, 636, 892
PleI	GAGTC	4 cut(s)	569, 667, 959, 1165
PpsI	GAGTC	4 cut(s)	569, 667, 959, 1165
PshAI	GACNNNNGTC	1 cut(s)	97
PspN4I	GGNNCC	5 cut(s)	129, 208, 617, 873, 993
PspOMI	GGGCCC	1 cut(s)	615
PspPI	GGNCC	5 cut(s)	207, 499, 530, 615, 616
PspXI	VCTCGAGB	1 cut(s)	602
PsuI	RGATCY	2 cut(s)	962, 1215
PsyI	GACNNNGTC	1 cut(s)	1063
RsaI	GTAC	4 cut(s)	177, 222, 389, 1128
RsaNI	GTAC	4 cut(s)	176, 221, 388, 1127
RseI	CAYNNNNRTG	1 cut(s)	1031
SaqAI	TTAA	3 cut(s)	924, 1029, 1041
SatI	GCNGC	4 cut(s)	113, 548, 635, 891
Sau3AI	GATC	4 cut(s)	962, 975, 1083, 1215
Sau96I	GGNCC	5 cut(s)	207, 499, 530, 615, 616
ScaI	AGTACT	1 cut(s)	389
SchI	GAGTC	4 cut(s)	569, 667, 960, 1166
ScrFI	CCNGG	1 cut(s)	571
SduI	GDGCHC	1 cut(s)	619
SfcI	CTRYAG	2 cut(s)	735, 1103
Sfr274I	CTCGAG	1 cut(s)	602
SfuI	TTCGAA	4 cut(s)	8, 486, 690, 1233
SinI	GGWCC	2 cut(s)	207, 530
SlaI	CTCGAG	1 cut(s)	602
SmiMI	CAYNNNNRTG	1 cut(s)	1031
SmlI	CTYRAG	2 cut(s)	383, 602
SmoI	CTYRAG	2 cut(s)	383, 602
SphI	GCATGC	1 cut(s)	902
SsiI	CCGC	2 cut(s)	579, 679
SspMI	CTAG	2 cut(s)	90, 527
StyD4I	CCNGG	1 cut(s)	569
StyI	CCWWGG	3 cut(s)	76, 246, 1277
TaaI	ACNGT	3 cut(s)	263, 295, 838
TaiI	ACGT	1 cut(s)	1067
TaqI	TCGA	8 cut(s)	8, 486, 603, 690, 699, 998, 1056, 1233
TatI	WGTACW	2 cut(s)	387, 1126
TfiI	GAWTC	3 cut(s)	5, 692, 704
Tru1I	TTAA	3 cut(s)	924, 1029, 1041
Tru9I	TTAA	3 cut(s)	924, 1029, 1041
TscAI	CASTG	4 cut(s)	298, 841, 1086, 1268
TseI	GCWGC	4 cut(s)	112, 547, 634, 890
TspDTI	ATGAA	2 cut(s)	630, 1111
TspRI	CASTG	4 cut(s)	298, 841, 1086, 1268
Tth111I	GACNNNGTC	1 cut(s)	1063
Van91I	CCANNNNNTGG	2 cut(s)	792, 881
VpaK11BI	GGWCC	2 cut(s)	207, 530
XapI	RAATTY	2 cut(s)	482, 1171
XceI	RCATGY	2 cut(s)	902, 1261
XhoI	CTCGAG	1 cut(s)	602
XmiI	GTMKAC	1 cut(s)	258
XspI	CTAG	2 cut(s)	90, 527
ZraI	GACGTC	1 cut(s)	1065
ZrmI	AGTACT	1 cut(s)	389

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.