Rw4G018000

Stem-specific protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012211
Physical Location & Seq
Reverse (-)
40180575 .. 40183781
3207 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G018000.1

Sequence Viewer

Length: 711 bp
ATGTTGGGAGTGTTCAGCAGCGCGATCGTGTCCCCTCCCGACGAGCTCGTCGCCGCCGGCAGCCGCACGCCGTCGCCGAAGATCACGGCGGATGCTCTGATCAACCGCTTCGTCGAGAGCAATGCCTCCGCCGTGTCCATACAGGTCGGCGACAACGTCCAGCTGGCCTATAGCCACGACAACGAGTCCCCGATACAACCGAGGTCGTTTGCTGTTAAGGATGAGGTATTCTGCTTGTTTGAGGGAGCACTTGACAACTTGGGAAGCTTGAGGCAGCAATATGGCCTGGCCAAGTCTGCAAATGAAGTGATTTTGGTCATTGAGGCTTACAAGGCGCTTCGCGATCGGGCACCTTACCCACCCAACCATGTTGTTGGCCATCTCCACGGAAATTTTGCTTTCATTGTATTTGACAAGTCCACCTCCACCGTGTTTGTTGCTTCTGACCAATTTGGCAAGGTCCCTCTCTCCTGGGGAATCACTGCTGATGGATATGTGGCCTTTGCTGATGATGCGGAGTTACTTAAGGGTGCTTGTGGCAAGTCACTTGCTTCATTCCCTCAAGGGTGTTTCTACTCCACAAGTGTTGGAGGACTGAGAAGCTATGAGAATCCTAAAAATGTAATCACTGCTGTACCGGCTACTGATGAAGAGATCTGGGGTGCAACATTTAAGGTTGAAGGGCCAGCAGTTTTTGCAGCCACAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3700

Protein Analysis

236

Amino Acids

25.14

Weight (kDa)

5.07

Isoelectric Point (pI)

33.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3700 PF12481 2 - 226 2.7e-94 Aluminium induced protein
GATase_7 PF13537 59 - 173 5.5e-11 Glutamine amidotransferase domain
GATase_6 PF13522 59 - 170 5.5e-10 Glutamine amidotransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012211)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19140 AT5G19140 AT5G19140
fragaria_vesca FvH4_4g14740 FvH4_4g14740
malus_domestica MD13G1185100.v1.1 MD16G1185700.v1.1
prunus_persica Prupe.1G152400_v2.0.a1 Prupe.1G152400_v2.0.a1
pyrus_communis pycom13g15970 pycom16g15550
rosa_chinensis RchiOBHm_Chr4g0417861
rosa_laevigata RLG00000007892
rosa_multiflora Rmu_sc0004911.1_g000008
rosa_roxburghii Rroxscaffold_5G00361280
rosa_rugosa Rorug04G0149900
rosa_samantha Rh4BG218800 Rh4DG210100
rosa_wichuraiana Rw4G018000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 47
AccB1I GGYRCC 1 cut(s) 349
AccII CGCG 2 cut(s) 23, 342
AciI CCGC 6 cut(s) 54, 64, 89, 106, 129, 515
AcoI YGGCCR 2 cut(s) 288, 376
AcsI RAATTY 1 cut(s) 391
AfaI GTAC 1 cut(s) 636
AflII CTTAAG 1 cut(s) 524
AgsI TTSAA 1 cut(s) 680
AhdI GACNNNNNGTC 1 cut(s) 184
AjnI CCWGG 2 cut(s) 285, 470
AluBI AGCT 4 cut(s) 46, 163, 267, 603
AluI AGCT 4 cut(s) 46, 163, 267, 603
Alw21I GWGCWC 2 cut(s) 48, 250
AoxI GGCC 6 cut(s) 165, 283, 288, 376, 498, 683
ApeKI GCWGC 4 cut(s) 18, 60, 274, 698
ApoI RAATTY 1 cut(s) 391
AspLEI GCGC 2 cut(s) 23, 337
AspS9I GGNCC 2 cut(s) 460, 683
AvaII GGWCC 1 cut(s) 460
BaeGI GKGCMC 1 cut(s) 352
BaeI ACNNNNGTAYC 2 cut(s) 618, 651
BalI TGGCCA 2 cut(s) 290, 378
BanI GGYRCC 1 cut(s) 349
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 2 cut(s) 48, 250
BbvI GCAGC 3 cut(s) 30, 72, 286
BccI CCATC 2 cut(s) 387, 482
BceAI ACGGC 3 cut(s) 55, 102, 116
BcgI CGANNNNNNTGC 4 cut(s) 7, 41, 104, 138
BciT130I CCWGG 2 cut(s) 287, 472
BclI TGATCA 1 cut(s) 99
BfmI CTRYAG 1 cut(s) 169
BfoI RGCGCY 1 cut(s) 338
BfrI CTTAAG 1 cut(s) 524
BglII AGATCT 1 cut(s) 654
BisI GCNGC 6 cut(s) 19, 54, 61, 64, 275, 699
BlsI GCNGC 6 cut(s) 20, 55, 62, 65, 276, 700
Bme1390I CCNGG 2 cut(s) 287, 472
Bme18I GGWCC 1 cut(s) 460
BmeRI GACNNNNNGTC 1 cut(s) 184
BmgT120I GGNCC 2 cut(s) 460, 683
BmiI GGNNCC 2 cut(s) 351, 462
BmrFI CCNGG 2 cut(s) 287, 472
BmsI GCATC 2 cut(s) 82, 502
BpuEI CTTGAG 2 cut(s) 289, 546
BsaJI CCNNGG 3 cut(s) 200, 385, 471
Bse118I RCCGGY 2 cut(s) 56, 637
Bse3DI GCAATG 1 cut(s) 127
BseBI CCWGG 2 cut(s) 287, 472
BseDI CCNNGG 3 cut(s) 200, 385, 471
BseGI GGATG 2 cut(s) 97, 226
BseMI GCAATG 1 cut(s) 127
BseMII CTCAG 1 cut(s) 587
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 3 cut(s) 30, 72, 286
Bsh1236I CGCG 2 cut(s) 23, 342
Bsh1285I CGRYCG 2 cut(s) 27, 346
BshFI GGCC 6 cut(s) 167, 285, 290, 378, 500, 685
BshNI GGYRCC 1 cut(s) 349
BsiEI CGRYCG 2 cut(s) 27, 346
BsiHKAI GWGCWC 2 cut(s) 48, 250
BsiSI CCGG 2 cut(s) 57, 638
BslFI GGGAC 3 cut(s) 16, 172, 446
BsmFI GGGAC 3 cut(s) 16, 172, 446
BsnI GGCC 6 cut(s) 167, 285, 290, 378, 500, 685
Bsp1286I GDGCHC 3 cut(s) 48, 250, 352
Bsp143I GATC 5 cut(s) 24, 81, 99, 343, 654
Bsp68I TCGCGA 1 cut(s) 342
BspACI CCGC 6 cut(s) 54, 64, 89, 106, 129, 515
BspANI GGCC 6 cut(s) 167, 285, 290, 378, 500, 685
BspCNI CTCAG 1 cut(s) 588
BspFNI CGCG 2 cut(s) 23, 342
BspLI GGNNCC 2 cut(s) 351, 462
BspT107I GGYRCC 1 cut(s) 349
BspTI CTTAAG 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 127
BsrFI RCCGGY 2 cut(s) 56, 637
BssAI RCCGGY 2 cut(s) 56, 637
BssECI CCNNGG 3 cut(s) 200, 385, 471
BssMI GATC 5 cut(s) 24, 81, 99, 343, 654
Bst2UI CCWGG 2 cut(s) 287, 472
Bst4CI ACNGT 1 cut(s) 430
Bst6I CTCTTC 1 cut(s) 645
BstAFI CTTAAG 1 cut(s) 524
BstAPI GCANNNNNTGC 1 cut(s) 695
BstC8I GCNNGC 4 cut(s) 58, 68, 165, 687
BstDEI CTNAG 1 cut(s) 596
BstDSI CCRYGG 1 cut(s) 385
BstF5I GGATG 2 cut(s) 97, 226
BstFNI CGCG 2 cut(s) 23, 342
BstH2I RGCGCY 1 cut(s) 338
BstHHI GCGC 2 cut(s) 23, 337
BstKTI GATC 5 cut(s) 27, 84, 102, 346, 657
BstMBI GATC 5 cut(s) 24, 81, 99, 343, 654
BstMCI CGRYCG 2 cut(s) 27, 346
BstMWI GCNNNNNNNGC 5 cut(s) 296, 332, 512, 638, 695
BstNI CCWGG 2 cut(s) 287, 472
BstSCI CCNGG 2 cut(s) 285, 470
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 2 cut(s) 23, 342
BstV1I GCAGC 3 cut(s) 30, 72, 286
BstX2I RGATCY 1 cut(s) 654
BstXI CCANNNNNNTGG 1 cut(s) 374
BstYI RGATCY 1 cut(s) 654
BsuRI GGCC 6 cut(s) 167, 285, 290, 378, 500, 685
BtgI CCRYGG 1 cut(s) 385
BtsCI GGATG 2 cut(s) 97, 226
BtsI GCAGTG 2 cut(s) 480, 627
BtsIMutI CAGTG 2 cut(s) 480, 627
BtuMI TCGCGA 1 cut(s) 342
Cac8I GCNNGC 4 cut(s) 58, 68, 165, 687
CfoI GCGC 2 cut(s) 23, 337
Cfr10I RCCGGY 2 cut(s) 56, 637
Cfr13I GGNCC 2 cut(s) 460, 683
Csp6I GTAC 1 cut(s) 635
CspCI CAANNNNNGTGG 4 cut(s) 415, 450, 568, 603
CviAII CATG 1 cut(s) 368
CviQI GTAC 1 cut(s) 635
DdeI CTNAG 1 cut(s) 596
DpnI GATC 5 cut(s) 26, 83, 101, 345, 656
DpnII GATC 5 cut(s) 24, 81, 99, 343, 654
DrdI GACNNNNNNGTC 1 cut(s) 47
DriI GACNNNNNGTC 1 cut(s) 184
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 2 cut(s) 288, 376
Eam1104I CTCTTC 1 cut(s) 645
Eam1105I GACNNNNNGTC 1 cut(s) 184
EarI CTCTTC 1 cut(s) 645
EciI GGCGGA 2 cut(s) 104, 118
Ecl136II GAGCTC 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 460
Eco53kI GAGCTC 1 cut(s) 46
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 460
EcoRII CCWGG 2 cut(s) 285, 470
EcoT38I GRGCYC 1 cut(s) 48
FaeI CATG 1 cut(s) 371
FaiI YATR 6 cut(s) 140, 171, 282, 369, 495, 606
FaqI GGGAC 3 cut(s) 16, 172, 446
FatI CATG 1 cut(s) 367
FbaI TGATCA 1 cut(s) 99
Fnu4HI GCNGC 6 cut(s) 19, 54, 61, 64, 275, 699
FokI GGATG 2 cut(s) 104, 233
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 6 cut(s) 19, 54, 61, 64, 275, 699
GlaI GCGC 2 cut(s) 22, 336
GluI GCNGC 6 cut(s) 19, 54, 61, 64, 275, 699
HaeII RGCGCY 1 cut(s) 338
HaeIII GGCC 6 cut(s) 167, 285, 290, 378, 500, 685
HapII CCGG 2 cut(s) 57, 638
HhaI GCGC 2 cut(s) 23, 337
Hin1II CATG 1 cut(s) 371
Hin6I GCGC 2 cut(s) 21, 335
HinP1I GCGC 2 cut(s) 21, 335
HindIII AAGCTT 1 cut(s) 265
HinfI GANTC 3 cut(s) 185, 477, 610
HpaII CCGG 2 cut(s) 57, 638
Hpy166II GTNNAC 1 cut(s) 420
Hpy188I TCNGA 2 cut(s) 99, 445
Hpy188III TCNNGA 3 cut(s) 38, 115, 341
Hpy8I GTNNAC 1 cut(s) 420
Hpy99I CGWCG 4 cut(s) 44, 53, 76, 116
HpyAV CCTTC 1 cut(s) 674
HpyCH4III ACNGT 1 cut(s) 430
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 3 cut(s) 299, 665, 698
HpyF10VI GCNNNNNNNGC 5 cut(s) 296, 332, 512, 638, 695
HpyF3I CTNAG 1 cut(s) 596
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 1 cut(s) 371
HspAI GCGC 2 cut(s) 21, 335
KroI GCCGGC 1 cut(s) 56
KroNI GCCGGC 1 cut(s) 58
Ksp22I TGATCA 1 cut(s) 99
Kzo9I GATC 5 cut(s) 24, 81, 99, 343, 654
LmnI GCTCC 1 cut(s) 245
Lsp1109I GCAGC 3 cut(s) 30, 72, 286
LweI GCATC 2 cut(s) 82, 502
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 519, 543
MalI GATC 5 cut(s) 26, 83, 101, 345, 656
MboI GATC 5 cut(s) 24, 81, 99, 343, 654
MboII GAAGA 2 cut(s) 91, 662
MflI RGATCY 1 cut(s) 654
MhlI GDGCHC 3 cut(s) 48, 250, 352
MlsI TGGCCA 2 cut(s) 290, 378
MluCI AATT 2 cut(s) 391, 449
MluNI TGGCCA 2 cut(s) 290, 378
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 568
Mox20I TGGCCA 2 cut(s) 290, 378
MroNI GCCGGC 1 cut(s) 56
MscI TGGCCA 2 cut(s) 290, 378
MseI TTAA 3 cut(s) 216, 525, 672
Msp20I TGGCCA 2 cut(s) 290, 378
MspA1I CMGCKG 1 cut(s) 163
MspCI CTTAAG 1 cut(s) 524
MspI CCGG 2 cut(s) 57, 638
MspR9I CCNGG 2 cut(s) 287, 472
MvaI CCWGG 2 cut(s) 287, 472
MvnI CGCG 2 cut(s) 23, 342
MwoI GCNNNNNNNGC 5 cut(s) 296, 332, 512, 638, 695
NaeI GCCGGC 1 cut(s) 58
NdeII GATC 5 cut(s) 24, 81, 99, 343, 654
NgoMIV GCCGGC 1 cut(s) 56
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 351, 462
NmuCI GTSAC 1 cut(s) 543
NruI TCGCGA 1 cut(s) 342
PcsI WCGNNNNNNNCGW 2 cut(s) 74, 153
PdiI GCCGGC 1 cut(s) 58
PfeI GAWTC 2 cut(s) 477, 610
PflFI GACNNNGTC 1 cut(s) 155
PkrI GCNGC 6 cut(s) 20, 55, 62, 65, 276, 700
Ple19I CGATCG 2 cut(s) 27, 346
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 460
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 460
Psp6I CCWGG 2 cut(s) 285, 470
PspGI CCWGG 2 cut(s) 285, 470
PspN4I GGNNCC 2 cut(s) 351, 462
PspPI GGNCC 2 cut(s) 460, 683
PspPPI RGGWCCY 1 cut(s) 460
PsuI RGATCY 1 cut(s) 654
PsyI GACNNNGTC 1 cut(s) 155
PvuI CGATCG 2 cut(s) 27, 346
PvuII CAGCTG 1 cut(s) 163
RruI TCGCGA 1 cut(s) 342
RsaI GTAC 1 cut(s) 636
RsaNI GTAC 1 cut(s) 635
SacI GAGCTC 1 cut(s) 48
SaqAI TTAA 3 cut(s) 216, 525, 672
SatI GCNGC 6 cut(s) 19, 54, 61, 64, 275, 699
Sau3AI GATC 5 cut(s) 24, 81, 99, 343, 654
Sau96I GGNCC 2 cut(s) 460, 683
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 2 cut(s) 287, 472
SduI GDGCHC 3 cut(s) 48, 250, 352
SfaNI GCATC 2 cut(s) 82, 502
SfcI CTRYAG 1 cut(s) 169
SinI GGWCC 1 cut(s) 460
SmlI CTYRAG 3 cut(s) 268, 524, 561
SmoI CTYRAG 3 cut(s) 268, 524, 561
Sse9I AATT 2 cut(s) 391, 449
SsiI CCGC 6 cut(s) 54, 64, 89, 106, 129, 515
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 2 cut(s) 285, 470
TaaI ACNGT 1 cut(s) 430
TaiI ACGT 1 cut(s) 159
TaqI TCGA 1 cut(s) 114
TasI AATT 2 cut(s) 391, 449
TauI GCSGC 2 cut(s) 56, 66
TfiI GAWTC 2 cut(s) 477, 610
Tru1I TTAA 3 cut(s) 216, 525, 672
Tru9I TTAA 3 cut(s) 216, 525, 672
TscAI CASTG 2 cut(s) 487, 634
TseFI GTSAC 1 cut(s) 543
TseI GCWGC 4 cut(s) 18, 60, 274, 698
Tsp45I GTSAC 1 cut(s) 543
TspDTI ATGAA 4 cut(s) 318, 391, 543, 663
TspGWI ACGGA 1 cut(s) 402
TspRI CASTG 2 cut(s) 487, 634
Tth111I GACNNNGTC 1 cut(s) 155
Vha464I CTTAAG 1 cut(s) 524
VpaK11BI GGWCC 1 cut(s) 460
XapI RAATTY 1 cut(s) 391
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.