Rw4G020060

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0012182
Physical Location & Seq
Forward (+)
43717524 .. 43718402
879 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G020060.1

Sequence Viewer

Length: 474 bp
ATGGCCAAGGACAGAGCAGAAGAGTTAAGCGAAGACGAAAAGAGGGCCTTAAGAGGCAGCAAATTCGCTCCTCTCCCATCTTTACCGCCTCCTCGATCCCAACCCAGGTTGGCTCATCCAGGGGGTCCGTTGACGACGAACAAGGCGGCGGCGTTGGCGAAATTTCTAGAAAGAAAGCTGCAGGATCCGGATGGATTGGGCACCATGAATCCACAGCTAGTTGAACGCGCTGTTCAGAACGCAAAACGCACCGTTTTCGCAAGTGCAGCTTCTAATTCTCAAGGAAGAATACGACATGTCAACTCCTTTGGTGACTCTGAGTCTGAGGAAGAAGAAGAAGAAGAAAATGTGGGAAACTCTGAGCCCAAAAAGAAGAAAAAGAATAAGAAGAAGAAGAAGAACAAGCAAAAGATAGTGGAGGACATTGGGGGTGCTGTGGTAAAAAAGCCGAAGAAGAAGAAGTTAAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.36

Weight (kDa)

9.99

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012182)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G18540 AT5G18540 AT5G18540 AT5G18540
fragaria_vesca FvH4_4g17670 FvH4_4g17670
malus_domestica MD13G1170800.v1.1
prunus_persica Prupe.1G139200_v2.0.a1
pyrus_communis pycom13g14650
rosa_chinensis RchiOBHm_Chr4g0421611
rosa_laevigata RLG00000007664
rosa_multiflora Rmu_ssc0000097.1_g000003
rosa_roxburghii Rroxscaffold_5G00363860
rosa_rugosa Rorug04G0173800
rosa_samantha Rh4AG233500 Rh4BG236400 Rh4CG248100 Rh4DG232300
rosa_wichuraiana Rw4G020060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 200
AccII CGCG 1 cut(s) 228
AccIII TCCGGA 1 cut(s) 187
AciI CCGC 3 cut(s) 86, 146, 149
AclWI GGATC 3 cut(s) 90, 179, 192
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 62, 161
AfiI CCNNNNNNNGG 1 cut(s) 105
AflII CTTAAG 1 cut(s) 49
AflIII ACRYGT 1 cut(s) 295
AgsI TTSAA 1 cut(s) 224
AhdI GACNNNNNGTC 1 cut(s) 319
AjnI CCWGG 2 cut(s) 104, 118
AluBI AGCT 3 cut(s) 178, 217, 269
AluI AGCT 3 cut(s) 178, 217, 269
AlwI GGATC 3 cut(s) 90, 179, 192
Aor13HI TCCGGA 1 cut(s) 187
AoxI GGCC 2 cut(s) 3, 45
ApeKI GCWGC 3 cut(s) 57, 178, 266
ApoI RAATTY 2 cut(s) 62, 161
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 45, 125
AsuHPI GGTGA 1 cut(s) 323
AvaII GGWCC 1 cut(s) 125
BaeGI GKGCMC 1 cut(s) 203
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 184
BanI GGYRCC 1 cut(s) 200
BanII GRGCYC 1 cut(s) 366
BbsI GAAGAC 1 cut(s) 39
BbvI GCAGC 3 cut(s) 69, 165, 278
BccI CCATC 2 cut(s) 85, 185
BcgI CGANNNNNNTGC 4 cut(s) 46, 80, 238, 272
BciT130I CCWGG 2 cut(s) 106, 120
BfaI CTAG 2 cut(s) 167, 218
BfmI CTRYAG 1 cut(s) 179
BfrI CTTAAG 1 cut(s) 49
BisI GCNGC 5 cut(s) 58, 147, 150, 179, 267
BlsI GCNGC 5 cut(s) 59, 148, 151, 180, 268
Bme1390I CCNGG 2 cut(s) 106, 120
Bme18I GGWCC 1 cut(s) 125
BmeRI GACNNNNNGTC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 45, 125
BmiI GGNNCC 3 cut(s) 126, 186, 202
BmrFI CCNGG 2 cut(s) 106, 120
BpiI GAAGAC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 264
BsaJI CCNNGG 3 cut(s) 6, 104, 119
BsaWI WCCGGW 1 cut(s) 187
Bsc4I CCNNNNNNNGG 1 cut(s) 105
BseAI TCCGGA 1 cut(s) 187
BseBI CCWGG 2 cut(s) 106, 120
BseDI CCNNGG 3 cut(s) 6, 104, 119
BseGI GGATG 2 cut(s) 115, 196
BseLI CCNNNNNNNGG 1 cut(s) 105
BseMII CTCAG 3 cut(s) 309, 315, 351
BseRI GAGGAG 2 cut(s) 60, 81
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 3 cut(s) 69, 165, 278
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 1 cut(s) 228
BshFI GGCC 2 cut(s) 5, 47
BshNI GGYRCC 1 cut(s) 200
BsiSI CCGG 1 cut(s) 188
BslI CCNNNNNNNGG 1 cut(s) 105
BsnI GGCC 2 cut(s) 5, 47
Bsp1286I GDGCHC 2 cut(s) 203, 366
Bsp13I TCCGGA 1 cut(s) 187
Bsp143I GATC 2 cut(s) 95, 184
BspACI CCGC 3 cut(s) 86, 146, 149
BspANI GGCC 2 cut(s) 5, 47
BspCNI CTCAG 3 cut(s) 310, 316, 352
BspEI TCCGGA 1 cut(s) 187
BspFNI CGCG 1 cut(s) 228
BspLI GGNNCC 3 cut(s) 126, 186, 202
BspMAI CTGCAG 1 cut(s) 183
BspPI GGATC 3 cut(s) 90, 179, 192
BspT107I GGYRCC 1 cut(s) 200
BspTI CTTAAG 1 cut(s) 49
BssECI CCNNGG 3 cut(s) 6, 104, 119
BssMI GATC 2 cut(s) 95, 184
BssT1I CCWWGG 1 cut(s) 6
Bst2UI CCWGG 2 cut(s) 106, 120
Bst4CI ACNGT 1 cut(s) 253
Bst6I CTCTTC 1 cut(s) 15
BstAFI CTTAAG 1 cut(s) 49
BstDEI CTNAG 3 cut(s) 318, 324, 360
BstF5I GGATG 2 cut(s) 115, 196
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 2 cut(s) 98, 187
BstMBI GATC 2 cut(s) 95, 184
BstMWI GCNNNNNNNGC 2 cut(s) 155, 266
BstNI CCWGG 2 cut(s) 106, 120
BstNSI RCATGY 1 cut(s) 299
BstSCI CCNGG 2 cut(s) 104, 118
BstSFI CTRYAG 1 cut(s) 179
BstSLI GKGCMC 1 cut(s) 203
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 3 cut(s) 69, 165, 278
BstV2I GAAGAC 1 cut(s) 39
BstX2I RGATCY 1 cut(s) 184
BstYI RGATCY 1 cut(s) 184
BsuRI GGCC 2 cut(s) 5, 47
BtsCI GGATG 2 cut(s) 115, 196
CfoI GCGC 1 cut(s) 230
Cfr13I GGNCC 2 cut(s) 45, 125
CviAII CATG 2 cut(s) 205, 296
CviJI RGCY 8 cut(s) 5, 47, 113, 178, 217, 269, 364, 448
CviKI_1 RGCY 8 cut(s) 5, 47, 113, 178, 217, 269, 364, 448
DdeI CTNAG 3 cut(s) 318, 324, 360
DpnI GATC 2 cut(s) 97, 186
DpnII GATC 2 cut(s) 95, 184
DriI GACNNNNNGTC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 15
Eam1105I GACNNNNNGTC 1 cut(s) 319
EarI CTCTTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 6
Eco24I GRGCYC 1 cut(s) 366
Eco47I GGWCC 1 cut(s) 125
EcoO109I RGGNCCY 1 cut(s) 45
EcoRII CCWGG 2 cut(s) 104, 118
EcoT14I CCWWGG 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 366
ErhI CCWWGG 1 cut(s) 6
FaeI CATG 2 cut(s) 208, 299
FaiI YATR 2 cut(s) 206, 297
FalI AAGNNNNNCTT 4 cut(s) 32, 64, 253, 285
FatI CATG 2 cut(s) 204, 295
Fnu4HI GCNGC 5 cut(s) 58, 147, 150, 179, 267
FokI GGATG 2 cut(s) 102, 203
FriOI GRGCYC 1 cut(s) 366
Fsp4HI GCNGC 5 cut(s) 58, 147, 150, 179, 267
FspBI CTAG 2 cut(s) 167, 218
GlaI GCGC 1 cut(s) 229
GluI GCNGC 5 cut(s) 58, 147, 150, 179, 267
HaeIII GGCC 2 cut(s) 5, 47
HapII CCGG 1 cut(s) 188
HhaI GCGC 1 cut(s) 230
Hin1II CATG 2 cut(s) 208, 299
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 2 cut(s) 132, 301
HindII GTYRAC 2 cut(s) 132, 301
HinfI GANTC 3 cut(s) 208, 314, 320
HpaII CCGG 1 cut(s) 188
HphI GGTGA 1 cut(s) 323
Hpy166II GTNNAC 2 cut(s) 132, 301
Hpy188I TCNGA 4 cut(s) 237, 319, 325, 361
Hpy188III TCNNGA 2 cut(s) 167, 188
Hpy8I GTNNAC 2 cut(s) 132, 301
Hpy99I CGWCG 1 cut(s) 139
HpyCH4III ACNGT 1 cut(s) 253
HpyCH4V TGCA 2 cut(s) 181, 266
HpyF10VI GCNNNNNNNGC 2 cut(s) 155, 266
HpyF3I CTNAG 3 cut(s) 318, 324, 360
Hsp92II CATG 2 cut(s) 208, 299
HspAI GCGC 1 cut(s) 228
Kpn2I TCCGGA 1 cut(s) 187
Kzo9I GATC 2 cut(s) 95, 184
LmnI GCTCC 1 cut(s) 73
LpnPI CCDG 6 cut(s) 91, 105, 118, 132, 167, 201
Lsp1109I GCAGC 3 cut(s) 69, 165, 278
MaeI CTAG 2 cut(s) 167, 218
MaeIII GTNAC 1 cut(s) 311
MalI GATC 2 cut(s) 97, 186
MboI GATC 2 cut(s) 95, 184
MflI RGATCY 1 cut(s) 184
MhlI GDGCHC 2 cut(s) 203, 366
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 62, 161, 274, 467
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 308, 329
MnlI CCTC 7 cut(s) 36, 47, 81, 99, 102, 319, 412
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 187
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 26, 50, 464
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 49
MspI CCGG 1 cut(s) 188
MspR9I CCNGG 2 cut(s) 106, 120
MvaI CCWGG 2 cut(s) 106, 120
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 2 cut(s) 155, 266
NdeII GATC 2 cut(s) 95, 184
NlaIII CATG 2 cut(s) 208, 299
NlaIV GGNNCC 3 cut(s) 126, 186, 202
NmuCI GTSAC 1 cut(s) 311
NspI RCATGY 1 cut(s) 299
PciI ACATGT 1 cut(s) 295
PfeI GAWTC 1 cut(s) 208
PkrI GCNGC 5 cut(s) 59, 148, 151, 180, 268
PleI GAGTC 2 cut(s) 308, 328
PpsI GAGTC 2 cut(s) 308, 328
PscI ACATGT 1 cut(s) 295
Psp6I CCWGG 2 cut(s) 104, 118
PspGI CCWGG 2 cut(s) 104, 118
PspN4I GGNNCC 3 cut(s) 126, 186, 202
PspPI GGNCC 2 cut(s) 45, 125
PstI CTGCAG 1 cut(s) 183
PsuI RGATCY 1 cut(s) 184
SaqAI TTAA 3 cut(s) 26, 50, 464
SatI GCNGC 5 cut(s) 58, 147, 150, 179, 267
Sau3AI GATC 2 cut(s) 95, 184
Sau96I GGNCC 2 cut(s) 45, 125
SchI GAGTC 2 cut(s) 308, 329
ScrFI CCNGG 2 cut(s) 106, 120
SduI GDGCHC 2 cut(s) 203, 366
SetI ASST 4 cut(s) 110, 180, 219, 271
SfcI CTRYAG 1 cut(s) 179
SinI GGWCC 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 49, 279
SmoI CTYRAG 2 cut(s) 49, 279
Sse9I AATT 4 cut(s) 62, 161, 274, 467
SsiI CCGC 3 cut(s) 86, 146, 149
SspMI CTAG 2 cut(s) 167, 218
StyD4I CCNGG 2 cut(s) 104, 118
StyI CCWWGG 1 cut(s) 6
TaaI ACNGT 1 cut(s) 253
TaqI TCGA 1 cut(s) 94
TasI AATT 4 cut(s) 62, 161, 274, 467
TauI GCSGC 2 cut(s) 149, 152
TfiI GAWTC 1 cut(s) 208
Tru1I TTAA 3 cut(s) 26, 50, 464
Tru9I TTAA 3 cut(s) 26, 50, 464
TseFI GTSAC 1 cut(s) 311
TseI GCWGC 3 cut(s) 57, 178, 266
Tsp45I GTSAC 1 cut(s) 311
TspDTI ATGAA 1 cut(s) 221
TspGWI ACGGA 1 cut(s) 117
Vha464I CTTAAG 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 125
XapI RAATTY 2 cut(s) 62, 161
XbaI TCTAGA 1 cut(s) 166
XceI RCATGY 1 cut(s) 299
XspI CTAG 2 cut(s) 167, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.