Rw4G031390

Carbohydrate-binding protein of the ER

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0018027
Physical Location & Seq
Forward (+)
58391943 .. 58394468
2526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031390.1

Sequence Viewer

Length: 2391 bp
ATGTTTACCATATTCTTGTTCATTTACCATCTAATAGCCTTCGTTTCAGCACACAGATCAGATTATGTGCTAGCAGATGACATGGTAATCGACTGTGGCCCGTTGTCAACATCCAAAGTTGGGTTTCTAGAGCCGGTGTCTCCACTATCCACCATTGATGCTGAGAGGAAGATTGGAGCTTCTAGTCATATACCTGGACCTATTCTCTTTCAAACGAACTACACTTACACCTGTATCGTCCCTCCAGGTCCCAAAATCATTCAATTCCAGTTCTATCCAACCTCACTGGAAAGCATAAACAAATCCGAAGCATTATTTATCATCAGTGCTGGAAAGTACACTTTGTTGAAGACTTCAGCATCCTCCTACTCTATAGGTTCTCAAGGTCAAATCCAGCATGCCATCAGAGAATTCTGCATCAACGTAGATCATGGAAGACTACACGTGACATTCACGCCATCATCAACTCCTGGTGCCTATGCATTTGTAAACAAGATCAAAGTTGTTTCGGTGCCGACAAGTCTTTATGTGAGAAGAAATGAGGAAGTTCCCCTTCCAATTCGTCTGATCGGACATAATCCTAAAGACCATCAAGTTTACATCCTTAACAACTCAATGGCTCTTGAGACCATGTACCGAGTCAACGTAGGCGGGCCTTTCATCTCTCCTGATCTAGATGGTTCTGAGATGCTGTGCACCTGGTCCACAGACGAAACATATCTGAAGGTCCCAGCATACACTGCACCAGTAAAAGTATACACCACAACCAGGGTTTTATATGTTGATGGTAATACTAGCTGGTCATTCCCTCTTGATTCTGGATTCTATTACCTCATCAGATTTCATTTCTGCGAGATCAGTGAGAAGATTAAGCTAGGCGAAAGAGTTTTTAAAGTCTACATCAACTATCAAACAGCTGAGGACCATGCAGATGTAATCGAATGGAGCAATGGAGCTGGTCTTCCAGTACGTCGAGATTATGTCGTTGATTATGTTTCCGGGCTTAGCAATAGCACGCGGAATCTGGTGGTTGCAATAGAAAACACCAACTCAGAATTTGATGATCATCAGGGAATCTTAAATGGCTTGGAAATCTTCAAAATATCAGACACTACCAACAATCTTGCTGGGGCACATCCATTCGGTTTTGAAGTTGATCAGTACCAAAACATTTCAACATTTACTGCTGCACATATCTTTCGCCGAATAGGTATTACACTCGATCATGGCTATTCTGTTTGGTGTCCTTGTACTAACAGCGGTATTCCATTCCATAAAATTACCGGTAGGACAGCGATGGGTTCAGACCAATATTATTGTCGAAACTTCTCACTTGCTGAGATGAAGTCTGCATCAAACAACTTCTGTCAAGGTTTTCTGATTGGCAGTGGTGGATTTGGGAAGGTCTACAAGGGTTATATAGGAGGTGCTGGCTACACTGCAACTACTATTGCTATAAAGCGTGCAAATCCCACTTCGAAGCAAGGCTTACATGAATTCCAAACAGAAATCACCATGCTCTCCAAGCTACGACACCACCACTTGGTTTCTTTGATTGGATACTGCAAAGAAGACAGGGAAATGATACTTGTGTACGACTATATGGCACAAGGTACACTCCGTGATCGTCTTTATGGTAAGTACCAGAAACCCCCACTTCCTTGGAAGCAGAGGATCAAGATATGTATAGGAGCAGCACGAGGCCTGCACTATCTCCACACAGGGGCAAATGAAAAAATCATTCACCGGGATGTGAAGGCTAGTAACATATTGTTGGACAAAAAATTGGTGGCCAAAGTTTCAGATTTTGGATTGTCAAAAGTTGGCCCCGACCAGAATATGATTAGTGCCACTGCCCAATCATCAAATGGTGAGACTCCTCATGTTAGCACTGAAGTAAAGGGTAGTTTTGGATACCTGGACCCGGAATATTATCGAAGACAGAAACTGACCGAGAAATCAGGCATTTATGCATTTGGGGTAGTGTTGTTTGAAGTTGTGTGTGCTAGGCCAGCTGTGATACGCGTTGTAGAAAATGGGGAGCCAGAACAAGTAAACTTAAGTGAATGGGCACTAGAACATTGTAATCCTGGTCAGGAGGGGAAAGCAATTGATCACATCCTAGACCCGTCTCTATGGGGAAAGATCAATTCCGAGCAATGTTTGACAGATAGGGGAAGTGAGCGGCCGTCGATGGGGGACGTGTTGTGGAAGTTGGAGCTGGCATTGCTGCAGCAAGAAAGAGCTGATCAAGTAGAAAACTATCGAATCGGCCCTAGTTTTCCTACAGAGATCGATGGTCATAAACAACAGGTCATTATTCCAGTTGGCATTTCAGATCCAACCCCAGGGCTCAAGTTTTCTGAGATGATGCTACCCACTGGACGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Malectin_like Pkinase_Tyr

Protein Analysis

796

Amino Acids

88.88

Weight (kDa)

6.7

Isoelectric Point (pI)

30.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin_like PF12819 74 - 368 6.7e-13 Malectin-like domain
Malectin PF11721 241 - 329 6.1e-06 Malectin domain
Pkinase PF00069 460 - 680 6.6e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 461 - 668 6.5e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018027)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g29510
rosa_chinensis RchiOBHm_Chr4g0437771 RchiOBHm_Chr4g0437781
rosa_laevigata RLG00000006417
rosa_multiflora Rmu_sc0001538.1_g000005 Rmu_sc0001629.1_g000005
rosa_roxburghii Rroxscaffold_5G00378880
rosa_rugosa Rorug04G0303600
rosa_samantha Rh4BG366400 Rh4CG380500
rosa_wichuraiana Rw4G031390

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 473, 511
AccB7I CCANNNNNTGG 1 cut(s) 1543
AccBSI CCGCTC 1 cut(s) 2185
AccI GTMKAC 3 cut(s) 756, 897, 1407
AccII CGCG 2 cut(s) 1018, 2025
AciI CCGC 4 cut(s) 651, 1018, 1260, 2185
AclWI GGATC 2 cut(s) 1682, 2333
AcoI YGGCCR 2 cut(s) 1791, 2186
AcsI RAATTY 3 cut(s) 410, 1055, 1496
AcuI CTGAAG 3 cut(s) 339, 743, 1914
AcvI CACGTG 1 cut(s) 445
AdeI CACNNNGTG 1 cut(s) 1622
AfaI GTAC 8 cut(s) 338, 635, 969, 1163, 1252, 1595, 1615, 1643
AfiI CCNNNNNNNGG 7 cut(s) 120, 768, 1543, 1723, 1924, 2195, 2348
AflII CTTAAG 1 cut(s) 2059
AflIII ACRYGT 3 cut(s) 442, 2023, 2202
AgeI ACCGGT 1 cut(s) 1283
AgsI TTSAA 7 cut(s) 212, 263, 349, 1099, 1151, 1176, 1994
AjiI CACGTC 1 cut(s) 2203
AjnI CCWGG 8 cut(s) 193, 244, 469, 698, 767, 1917, 2089, 2347
AluBI AGCT 9 cut(s) 179, 798, 874, 917, 956, 1528, 2015, 2221, 2246
AluI AGCT 9 cut(s) 179, 798, 874, 917, 956, 1528, 2015, 2221, 2246
Alw21I GWGCWC 1 cut(s) 698
Alw26I GTCTC 4 cut(s) 144, 620, 1868, 2136
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 2 cut(s) 1682, 2333
AlwNI CAGNNNCTG 1 cut(s) 1948
AoxI GGCC 8 cut(s) 97, 653, 1702, 1791, 1825, 2009, 2186, 2272
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 4 cut(s) 1187, 1694, 2230, 2233
ApoI RAATTY 3 cut(s) 410, 1055, 1496
AsiGI ACCGGT 1 cut(s) 1283
AsuC2I CCSGG 3 cut(s) 1000, 1748, 1925
AsuHPI GGTGA 3 cut(s) 1504, 1736, 1883
AsuII TTCGAA 1 cut(s) 1478
AsuNHI GCTAGC 1 cut(s) 70
AvaII GGWCC 6 cut(s) 197, 248, 702, 727, 922, 1921
BaeGI GKGCMC 3 cut(s) 698, 1135, 2074
BaeI ACNNNNGTAYC 4 cut(s) 217, 250, 1577, 1610
BalI TGGCCA 1 cut(s) 1793
BanI GGYRCC 2 cut(s) 473, 511
BanII GRGCYC 1 cut(s) 2355
BauI CACGAG 1 cut(s) 1698
BbrPI CACGTG 1 cut(s) 445
BbsI GAAGAC 5 cut(s) 356, 442, 953, 1578, 1945
Bbv12I GWGCWC 1 cut(s) 698
BbvCI CCTCAGC 1 cut(s) 918
BbvI GCAGC 4 cut(s) 1174, 1706, 2217, 2245
BccI CCATC 9 cut(s) 36, 410, 466, 597, 671, 779, 1291, 2188, 2291
BceAI ACGGC 1 cut(s) 2173
BciT130I CCWGG 8 cut(s) 195, 246, 471, 700, 769, 1919, 2091, 2349
BciVI GTATCC 2 cut(s) 1553, 1907
BclI TGATCA 4 cut(s) 1063, 1156, 2113, 2248
BcnI CCSGG 3 cut(s) 1000, 1748, 1925
BcoDI GTCTC 4 cut(s) 144, 620, 1868, 2136
BfmI CTRYAG 3 cut(s) 372, 2231, 2286
BfrI CTTAAG 1 cut(s) 2059
BfuI GTATCC 2 cut(s) 1553, 1907
BisI GCNGC 5 cut(s) 1188, 1695, 2186, 2231, 2234
BlpI GCTNAGC 1 cut(s) 1004
BlsI GCNGC 5 cut(s) 1189, 1696, 2187, 2232, 2235
Bme18I GGWCC 6 cut(s) 197, 248, 702, 727, 922, 1921
BmgBI CACGTC 1 cut(s) 2203
BmiI GGNNCC 7 cut(s) 250, 475, 513, 729, 1828, 1923, 2043
BmsI GCATC 6 cut(s) 148, 368, 426, 678, 1361, 2361
BmtI GCTAGC 1 cut(s) 74
BpiI GAAGAC 5 cut(s) 356, 442, 953, 1578, 1945
BpmI CTGGAG 1 cut(s) 228
Bpu10I CCTNAGC 1 cut(s) 918
Bpu1102I GCTNAGC 1 cut(s) 1004
Bpu14I TTCGAA 1 cut(s) 1478
BpuEI CTTGAG 3 cut(s) 366, 644, 2339
BpuMI CCSGG 3 cut(s) 1000, 1748, 1925
Bsa29I ATCGAT 1 cut(s) 2295
BsaAI YACGTR 1 cut(s) 445
BsaBI GATNNNNATC 1 cut(s) 1065
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 4 cut(s) 768, 1661, 2347, 2348
BsaWI WCCGGW 1 cut(s) 1283
Bsc4I CCNNNNNNNGG 7 cut(s) 120, 768, 1543, 1723, 1924, 2195, 2348
Bse118I RCCGGY 2 cut(s) 133, 1283
Bse1I ACTGG 6 cut(s) 268, 291, 746, 965, 2324, 2386
Bse3DI GCAATG 3 cut(s) 955, 2165, 2225
Bse8I GATNNNNATC 1 cut(s) 1065
BseBI CCWGG 8 cut(s) 195, 246, 471, 700, 769, 1919, 2091, 2349
BseCI ATCGAT 1 cut(s) 2295
BseDI CCNNGG 4 cut(s) 768, 1661, 2347, 2348
BseGI GGATG 6 cut(s) 110, 359, 600, 1135, 1756, 2118
BseJI GATNNNNATC 1 cut(s) 1065
BseLI CCNNNNNNNGG 7 cut(s) 120, 768, 1543, 1723, 1924, 2195, 2348
BseMI GCAATG 3 cut(s) 955, 2165, 2225
BseMII CTCAG 6 cut(s) 153, 675, 909, 1065, 1329, 2355
BseNI ACTGG 6 cut(s) 268, 291, 746, 965, 2324, 2386
BseRI GAGGAG 1 cut(s) 1869
BseSI GKGCMC 3 cut(s) 698, 1135, 2074
BseX3I CGGCCG 1 cut(s) 2186
BseXI GCAGC 4 cut(s) 1174, 1706, 2217, 2245
BseYI CCCAGC 2 cut(s) 730, 1127
BsgI GTGCAG 3 cut(s) 726, 1173, 1691
Bsh1236I CGCG 2 cut(s) 1018, 2025
Bsh1285I CGRYCG 1 cut(s) 2189
BshFI GGCC 8 cut(s) 99, 655, 1704, 1793, 1827, 2011, 2188, 2274
BshNI GGYRCC 2 cut(s) 473, 511
BshTI ACCGGT 1 cut(s) 1283
BshVI ATCGAT 1 cut(s) 2295
BsiEI CGRYCG 1 cut(s) 2189
BsiHKAI GWGCWC 1 cut(s) 698
BsiSI CCGG 5 cut(s) 134, 999, 1284, 1747, 1925
BslFI GGGAC 4 cut(s) 224, 234, 713, 2213
BslI CCNNNNNNNGG 7 cut(s) 120, 768, 1543, 1723, 1924, 2195, 2348
BsmAI GTCTC 4 cut(s) 144, 620, 1868, 2136
BsmBI CGTCTC 1 cut(s) 2136
BsmFI GGGAC 4 cut(s) 224, 234, 713, 2213
BsnI GGCC 8 cut(s) 99, 655, 1704, 1793, 1827, 2011, 2188, 2274
Bso31I GGTCTC 1 cut(s) 620
Bsp119I TTCGAA 1 cut(s) 1478
Bsp1286I GDGCHC 4 cut(s) 698, 1135, 2074, 2355
Bsp1720I GCTNAGC 1 cut(s) 1004
BspACI CCGC 4 cut(s) 651, 1018, 1260, 2185
BspANI GGCC 8 cut(s) 99, 655, 1704, 1793, 1827, 2011, 2188, 2274
BspCNI CTCAG 6 cut(s) 154, 676, 910, 1064, 1330, 2356
BspDI ATCGAT 1 cut(s) 2295
BspFNI CGCG 2 cut(s) 1018, 2025
BspLI GGNNCC 7 cut(s) 250, 475, 513, 729, 1828, 1923, 2043
BspMAI CTGCAG 1 cut(s) 2235
BspOI GCTAGC 1 cut(s) 74
BspPI GGATC 2 cut(s) 1682, 2333
BspT104I TTCGAA 1 cut(s) 1478
BspT107I GGYRCC 2 cut(s) 473, 511
BspTI CTTAAG 1 cut(s) 2059
BspTNI GGTCTC 1 cut(s) 620
BsrBI CCGCTC 1 cut(s) 2185
BsrDI GCAATG 3 cut(s) 955, 2165, 2225
BsrFI RCCGGY 2 cut(s) 133, 1283
BsrI ACTGG 6 cut(s) 268, 291, 746, 965, 2324, 2386
BssAI RCCGGY 2 cut(s) 133, 1283
BssECI CCNNGG 4 cut(s) 768, 1661, 2347, 2348
BssNAI GTATAC 1 cut(s) 757
BssSI CACGAG 1 cut(s) 1698
BssT1I CCWWGG 1 cut(s) 1661
Bst1107I GTATAC 1 cut(s) 757
Bst2BI CACGAG 1 cut(s) 1698
Bst2UI CCWGG 8 cut(s) 195, 246, 471, 700, 769, 1919, 2091, 2349
Bst4CI ACNGT 1 cut(s) 95
BstAFI CTTAAG 1 cut(s) 2059
BstAPI GCANNNNNTGC 1 cut(s) 740
BstBAI YACGTR 1 cut(s) 445
BstBI TTCGAA 1 cut(s) 1478
BstC8I GCNNGC 9 cut(s) 72, 399, 653, 1016, 1432, 1464, 1706, 2013, 2223
BstDEI CTNAG 7 cut(s) 162, 684, 918, 1004, 1051, 1338, 2364
BstF5I GGATG 6 cut(s) 110, 359, 600, 1135, 1756, 2118
BstFNI CGCG 2 cut(s) 1018, 2025
BstMAI GTCTC 4 cut(s) 144, 620, 1868, 2136
BstMCI CGRYCG 1 cut(s) 2189
BstMWI GCNNNNNNNGC 4 cut(s) 740, 1525, 2012, 2227
BstNI CCWGG 8 cut(s) 195, 246, 471, 700, 769, 1919, 2091, 2349
BstNSI RCATGY 1 cut(s) 401
BstSFI CTRYAG 3 cut(s) 372, 2231, 2286
BstSLI GKGCMC 3 cut(s) 698, 1135, 2074
BstUI CGCG 2 cut(s) 1018, 2025
BstV1I GCAGC 4 cut(s) 1174, 1706, 2217, 2245
BstV2I GAAGAC 5 cut(s) 356, 442, 953, 1578, 1945
BstX2I RGATCY 1 cut(s) 2338
BstXI CCANNNNNNTGG 1 cut(s) 1662
BstYI RGATCY 1 cut(s) 2338
BstZ17I GTATAC 1 cut(s) 757
BstZI CGGCCG 1 cut(s) 2186
Bsu15I ATCGAT 1 cut(s) 2295
BsuI GTATCC 2 cut(s) 1553, 1907
BsuRI GGCC 8 cut(s) 99, 655, 1704, 1793, 1827, 2011, 2188, 2274
BsuTUI ATCGAT 1 cut(s) 2295
BtgZI GCGATG 1 cut(s) 1310
BtrI CACGTC 1 cut(s) 2203
BtsCI GGATG 6 cut(s) 110, 359, 600, 1135, 1756, 2118
BtsI GCAGTG 4 cut(s) 738, 1393, 1437, 1851
BtsIMutI CAGTG 9 cut(s) 284, 331, 738, 865, 1393, 1437, 1851, 1890, 2379
Cac8I GCNNGC 9 cut(s) 72, 399, 653, 1016, 1432, 1464, 1706, 2013, 2223
CaiI CAGNNNCTG 1 cut(s) 1948
Cfr10I RCCGGY 2 cut(s) 133, 1283
ClaI ATCGAT 1 cut(s) 2295
CsiI ACCWGGT 1 cut(s) 698
Csp6I GTAC 8 cut(s) 337, 634, 968, 1162, 1251, 1594, 1614, 1642
CspAI ACCGGT 1 cut(s) 1283
CviAII CATG 9 cut(s) 82, 398, 431, 631, 926, 1226, 1493, 1516, 1883
CviQI GTAC 8 cut(s) 337, 634, 968, 1162, 1251, 1594, 1614, 1642
DdeI CTNAG 7 cut(s) 162, 684, 918, 1004, 1051, 1338, 2364
DraI TTTAAA 1 cut(s) 892
DraIII CACNNNGTG 1 cut(s) 1622
EaeI YGGCCR 2 cut(s) 1791, 2186
EagI CGGCCG 1 cut(s) 2186
EclXI CGGCCG 1 cut(s) 2186
Eco130I CCWWGG 1 cut(s) 1661
Eco147I AGGCCT 1 cut(s) 1704
Eco24I GRGCYC 1 cut(s) 2355
Eco31I GGTCTC 1 cut(s) 620
Eco47I GGWCC 6 cut(s) 197, 248, 702, 727, 922, 1921
Eco52I CGGCCG 1 cut(s) 2186
Eco57I CTGAAG 3 cut(s) 339, 743, 1914
Eco72I CACGTG 1 cut(s) 445
EcoO109I RGGNCCY 2 cut(s) 248, 727
EcoRI GAATTC 2 cut(s) 410, 1496
EcoRII CCWGG 8 cut(s) 193, 244, 469, 698, 767, 1917, 2089, 2347
EcoT14I CCWWGG 1 cut(s) 1661
EcoT22I ATGCAT 2 cut(s) 484, 1975
EcoT38I GRGCYC 1 cut(s) 2355
ErhI CCWWGG 1 cut(s) 1661
Esp3I CGTCTC 1 cut(s) 2136
FaeI CATG 9 cut(s) 85, 401, 434, 634, 929, 1229, 1496, 1519, 1886
FalI AAGNNNNNCTT 4 cut(s) 537, 569, 1472, 1504
FaqI GGGAC 4 cut(s) 224, 234, 713, 2213
FatI CATG 9 cut(s) 81, 397, 430, 630, 925, 1225, 1492, 1515, 1882
FauI CCCGC 1 cut(s) 644
FbaI TGATCA 4 cut(s) 1063, 1156, 2113, 2248
FblI GTMKAC 3 cut(s) 756, 897, 1407
Fnu4HI GCNGC 5 cut(s) 1188, 1695, 2186, 2231, 2234
FokI GGATG 6 cut(s) 97, 346, 587, 1122, 1763, 2105
FriOI GRGCYC 1 cut(s) 2355
Fsp4HI GCNGC 5 cut(s) 1188, 1695, 2186, 2231, 2234
GluI GCNGC 5 cut(s) 1188, 1695, 2186, 2231, 2234
GsaI CCCAGC 2 cut(s) 734, 1131
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 8 cut(s) 99, 655, 1704, 1793, 1827, 2011, 2188, 2274
HapII CCGG 5 cut(s) 134, 999, 1284, 1747, 1925
Hin1II CATG 9 cut(s) 85, 401, 434, 634, 929, 1229, 1496, 1519, 1886
HincII GTYRAC 2 cut(s) 108, 643
HindII GTYRAC 2 cut(s) 108, 643
HinfI GANTC 7 cut(s) 639, 815, 822, 1021, 1074, 1876, 2268
HpaII CCGG 5 cut(s) 134, 999, 1284, 1747, 1925
HphI GGTGA 3 cut(s) 1504, 1736, 1883
Hpy188III TCNNGA 9 cut(s) 128, 623, 668, 674, 812, 819, 974, 1678, 2096
Hpy99I CGWCG 2 cut(s) 975, 2194
HpyAV CCTTC 5 cut(s) 49, 563, 718, 1396, 1750
HpyCH4III ACNGT 1 cut(s) 95
HpyCH4IV ACGT 6 cut(s) 423, 444, 645, 970, 2202, 2386
HpyF10VI GCNNNNNNNGC 4 cut(s) 740, 1525, 2012, 2227
HpyF3I CTNAG 7 cut(s) 162, 684, 918, 1004, 1051, 1338, 2364
HpySE526I ACGT 6 cut(s) 423, 444, 645, 970, 2202, 2386
Hsp92II CATG 9 cut(s) 85, 401, 434, 634, 929, 1229, 1496, 1519, 1886
Ksp22I TGATCA 4 cut(s) 1063, 1156, 2113, 2248
LmnI GCTCC 6 cut(s) 176, 945, 953, 1691, 2041, 2218
Lsp1109I GCAGC 4 cut(s) 1174, 1706, 2217, 2245
LweI GCATC 6 cut(s) 148, 368, 426, 678, 1361, 2361
MabI ACCWGGT 1 cut(s) 698
MaeII ACGT 6 cut(s) 423, 444, 645, 970, 2202, 2386
MaeIII GTNAC 2 cut(s) 445, 1763
MbiI CCGCTC 1 cut(s) 2185
MboII GAAGA 9 cut(s) 181, 361, 447, 546, 877, 953, 1087, 1583, 1950
MfeI CAATTG 1 cut(s) 2109
MflI RGATCY 1 cut(s) 2338
MhlI GDGCHC 4 cut(s) 698, 1135, 2074, 2355
MlsI TGGCCA 1 cut(s) 1793
MluCI AATT 9 cut(s) 263, 410, 558, 1055, 1278, 1496, 1784, 2109, 2149
MluI ACGCGT 1 cut(s) 2023
MluNI TGGCCA 1 cut(s) 1793
MlyI GAGTC 2 cut(s) 648, 1870
MmeI TCCRAC 4 cut(s) 302, 1755, 2196, 2366
Mox20I TGGCCA 1 cut(s) 1793
Mph1103I ATGCAT 2 cut(s) 484, 1975
MscI TGGCCA 1 cut(s) 1793
MseI TTAA 5 cut(s) 606, 870, 891, 1079, 2060
MslI CAYNNNNRTG 2 cut(s) 930, 1749
Msp20I TGGCCA 1 cut(s) 1793
MspA1I CMGCKG 3 cut(s) 917, 1260, 2015
MspCI CTTAAG 1 cut(s) 2059
MspI CCGG 5 cut(s) 134, 999, 1284, 1747, 1925
MunI CAATTG 1 cut(s) 2109
MvaI CCWGG 8 cut(s) 195, 246, 471, 700, 769, 1919, 2091, 2349
MvnI CGCG 2 cut(s) 1018, 2025
MwoI GCNNNNNNNGC 4 cut(s) 740, 1525, 2012, 2227
NciI CCSGG 3 cut(s) 1000, 1748, 1925
NheI GCTAGC 1 cut(s) 70
NlaIII CATG 9 cut(s) 85, 401, 434, 634, 929, 1229, 1496, 1519, 1886
NlaIV GGNNCC 7 cut(s) 250, 475, 513, 729, 1828, 1923, 2043
NmuCI GTSAC 1 cut(s) 445
NsiI ATGCAT 2 cut(s) 484, 1975
NspI RCATGY 1 cut(s) 401
NspV TTCGAA 1 cut(s) 1478
PaeI GCATGC 1 cut(s) 401
PasI CCCWGGG 1 cut(s) 2348
PceI AGGCCT 1 cut(s) 1704
PfeI GAWTC 5 cut(s) 815, 822, 1021, 1074, 2268
PflMI CCANNNNNTGG 1 cut(s) 1543
PinAI ACCGGT 1 cut(s) 1283
PkrI GCNGC 5 cut(s) 1189, 1696, 2187, 2232, 2235
PleI GAGTC 2 cut(s) 647, 1870
PmaCI CACGTG 1 cut(s) 445
PmlI CACGTG 1 cut(s) 445
PpsI GAGTC 2 cut(s) 647, 1870
Ppu21I YACGTR 1 cut(s) 445
PpuMI RGGWCCY 2 cut(s) 248, 727
Psp5II RGGWCCY 2 cut(s) 248, 727
Psp6I CCWGG 8 cut(s) 193, 244, 469, 698, 767, 1917, 2089, 2347
PspCI CACGTG 1 cut(s) 445
PspFI CCCAGC 2 cut(s) 730, 1127
PspGI CCWGG 8 cut(s) 193, 244, 469, 698, 767, 1917, 2089, 2347
PspN4I GGNNCC 7 cut(s) 250, 475, 513, 729, 1828, 1923, 2043
PspPPI RGGWCCY 2 cut(s) 248, 727
PsrI GAACNNNNNNTAC 2 cut(s) 209, 241
PstI CTGCAG 1 cut(s) 2235
PstNI CAGNNNCTG 1 cut(s) 1948
PsuI RGATCY 1 cut(s) 2338
PvuII CAGCTG 2 cut(s) 917, 2015
RsaI GTAC 8 cut(s) 338, 635, 969, 1163, 1252, 1595, 1615, 1643
RsaNI GTAC 8 cut(s) 337, 634, 968, 1162, 1251, 1594, 1614, 1642
RseI CAYNNNNRTG 2 cut(s) 930, 1749
SaqAI TTAA 5 cut(s) 606, 870, 891, 1079, 2060
SatI GCNGC 5 cut(s) 1188, 1695, 2186, 2231, 2234
SchI GAGTC 2 cut(s) 648, 1870
SduI GDGCHC 4 cut(s) 698, 1135, 2074, 2355
SexAI ACCWGGT 1 cut(s) 698
SfaNI GCATC 6 cut(s) 148, 368, 426, 678, 1361, 2361
SfcI CTRYAG 3 cut(s) 372, 2231, 2286
SfuI TTCGAA 1 cut(s) 1478
SinI GGWCC 6 cut(s) 197, 248, 702, 727, 922, 1921
SmiMI CAYNNNNRTG 2 cut(s) 930, 1749
SmlI CTYRAG 4 cut(s) 381, 623, 2059, 2354
SmoI CTYRAG 4 cut(s) 381, 623, 2059, 2354
SphI GCATGC 1 cut(s) 401
Sse9I AATT 9 cut(s) 263, 410, 558, 1055, 1278, 1496, 1784, 2109, 2149
SseBI AGGCCT 1 cut(s) 1704
SsiI CCGC 4 cut(s) 651, 1018, 1260, 2185
SspI AATATT 2 cut(s) 1313, 1931
StuI AGGCCT 1 cut(s) 1704
StyI CCWWGG 1 cut(s) 1661
TaaI ACNGT 1 cut(s) 95
TaiI ACGT 6 cut(s) 426, 447, 648, 973, 2205, 2389
TaqII GACCGA 1 cut(s) 1967
TasI AATT 9 cut(s) 263, 410, 558, 1055, 1278, 1496, 1784, 2109, 2149
TatI WGTACW 2 cut(s) 336, 1250
TauI GCSGC 1 cut(s) 2188
TfiI GAWTC 5 cut(s) 815, 822, 1021, 1074, 2268
Tru1I TTAA 5 cut(s) 606, 870, 891, 1079, 2060
Tru9I TTAA 5 cut(s) 606, 870, 891, 1079, 2060
TscAI CASTG 9 cut(s) 291, 331, 745, 865, 1393, 1444, 1858, 1897, 2386
TseFI GTSAC 1 cut(s) 445
TseI GCWGC 4 cut(s) 1187, 1694, 2230, 2233
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 6 cut(s) 10, 649, 833, 1358, 1509, 1746
TspGWI ACGGA 1 cut(s) 1610
TspRI CASTG 9 cut(s) 291, 331, 745, 865, 1393, 1444, 1858, 1897, 2386
Van91I CCANNNNNTGG 1 cut(s) 1543
Vha464I CTTAAG 1 cut(s) 2059
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 6 cut(s) 197, 248, 702, 727, 922, 1921
XapI RAATTY 3 cut(s) 410, 1055, 1496
XbaI TCTAGA 2 cut(s) 127, 673
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 1865
XmiI GTMKAC 3 cut(s) 756, 897, 1407
Zsp2I ATGCAT 2 cut(s) 484, 1975
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.