Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr5

Orthogroup ID

OG0011536

Physical Location & Seq

Genomic Coordinates Forward (+)

5745847 .. 5751172

Gene Structure 5326 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw5G005490.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGGGGTTTAGGAGGTGGGGGATATGCATGCATGTCTTCTTTGTTGGGTTTGTGGTGATTCAGAGTATGGAGTCTTCTGTTGTTGCATTGACTGACCCAATAGATGTTGCTGCAGTGAATAGCTTATATGTTAATCTAGGCTATCCACCTCTTCAAGGGTGGCTTCTTGTTGGAGGAGACCCCTGTGGGGAGAACTGGCAAGGTGTTATTTGTGTCTTCGCGAATGTAACAGCACTAAAGCTTAGTAGTGCCAACTTGGGAGGACAACTGGGTGATAGTTTGCAGACCTTTGGGTCAATAATAGAAATAGATTTGAGCAATAATCACATTGGAGGGAATATTCCAATACTTTTGCCTCCGACTGTCAGAAACGTTTCTCTATCAAATAATCAGCTTAATGGAAGCATCCCAGGTGGTTTGGCATCATTATCTCAGTTGATGGAATTGTCATTAGATAATAACCATATCAGTGGAGAAATACCAGATGCCTTTCAACTGCTTAGTAGTTTGACGAGATTGGATCTGTCCGGTAATAATTTAACGGGTCAACTAGGTCCGTCATTGGGGAATTTGTCATCTCTTACCACATTGTACCTGCAGAACAATCACCTGACTGGAACCCTTGACGTGTTACAGGATCTTCCTCTACAGGATCTGAATATTGAAAATAATCTATTCTCTGGGCCAATACCAGCAAAGTTGCTGACTATTCCAGATTTCAGAAAAGAGGGAAATCCTTTCAACACCACTATTCTTCCATCACCTCCAGCACCAGCACCAGTAGCTTCATCAATAGCTAGTGCACCTTCTTCTGGAGCACGAGTAGAGAATCCGGCTAATGGGCCCTCTCCACTACCAATACTTATTTCTCATAGAACTGGAAAATCCTTGATAACTAAGCCGCTCACTTGGGTTGCAATTGGAGGGGTTTCATTAGTTCTGTTATTGATTTTGTGTGGACTAGTCAGATGTTTCAAAGCAAGGAGTGACAACAAGAAAGCAAAGAGGCATGACATTAGTGCATATAAGCTTCCTACAGAGAATACCAAATACACTCAATCTTCACTTGAGGAAGGTCTTCAAATGGAGAAAGTTCCAAAAGAGGCCATTTTGAAACCACTGGATGGATATGGAGCAGAGAGCATCAGAATGGGTATGATGGCAAAACCGCAAAAACATAAAAAGACTGATGTGAAAAGTACTGAAAGTTCAATGCATAGGAAAGGTCATGGGATTAACATGACAGACACGGATGAAGAATTTTTGCCGCCGCCACCTCCCCCCCCATTTTTCCTCAGCAAGAAGGATACTGTAAATCCCGTTGGTCCTGCTACCACAAGCAGGCTGAAGAGTCCAAACCCAAGCTCTGTAAAAGTCTTTACTATTGCATCACTTCAGCAGTATACCAATAGCTTTTCTGAAGAAAATTTTATTGGAGCAGGCATGCTTGGCAGTGTTTATAGGGCTAAGCTTCCTGATCAAAAGCTTTTAGCAGTCAAGAAACTGGACAGTACAGCTTGCAGCCAGCAGAGTGATGAGGAATTTCTTGAACTAGTGTCTAATATCTCTGAAATTAGACATGCTAATATTGTGGAACTAGTGGGCTACTGTGCGGAGCATGACCAACGGCTACTTGTGTATAAGTATTGCAGAAATGGGACTCTCCATGATGCACTGCATACTGATGATAATATTCACCAGAAACTCTCATGGACTGTGCGCATCCGTGTGGCACTTGGAGCAGCAAGAGCTCTTGAGTATTTGCATGCGGTTTGTCAACCACCAATTGTGCACCACAATTTCAAGTCTGCCAATCTTCTTCTTGATGAAAAGCTTGAAGTGCATGTCTCAGACTGTGGTTTGGCTCCTCTGATAACATCTCCCTCTTTGGCTGGACACCTCCTCACTGCCTATGGTTCTTCAGCCCCAGAATTTGATTCAGGATGTTATACTCACCAAAGTGATGTCTATAGCTTTGGAGTTGTAATGTTAGAGCTCCTTACAGGGCGAAAAGCCTATGACAGGTCAAGGCCTCGTGGTGAGCAATATTTGGTCAGGTGGGCAGTTCCTAAGCTTCATGACATTGATGCATTGTCAAGGATGGTTGATCCCTCTCTAAATGGAGCTTATACAATGAAGTCTTTGTCACGTTTGGCAGATATTATTTCCTCTTGCATACATCGAGAGCCAGAATTCCGACCACCTATATCTGAAATTGTCCAAGAACTCCTGCAAATGACTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0030054 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0055044 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

747

Amino Acids

81.33

Weight (kDa)

6.43

Isoelectric Point (pI)

46.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_8	PF13855	120 - 180	1.7e-09	Leucine rich repeat
Pkinase	PF00069	473 - 742	1.1e-34	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	476 - 743	6.1e-38	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011536)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g04500 FvH4_3g04500 FvH4_3g04500
malus_domestica	MD05G1322700.v1.1 MD10G1300600.v1.1
prunus_persica	Prupe.4G039900_v2.0.a1 Prupe.4G039900_v2.0.a1 Prupe.4G039900_v2.0.a1
pyrus_communis	pycom05g29700 pycom10g25350
rosa_chinensis	RchiOBHm_Chr5g0007431
rosa_laevigata	RLG00000031456
rosa_multiflora	Rmu_sc0003826.1_g000001
rosa_roxburghii	Rroxscaffold_1G00069040
rosa_rugosa	Rorug04G0426800
rosa_samantha	Rh5AG055200 Rh5BG052800 Rh5CG062600 Rh5DG051800
rosa_wichuraiana	Rw5G005490

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	292
Acc16I	TGCGCA	1 cut(s)	1721
Acc36I	ACCTGC	1 cut(s)	603
AccB7I	CCANNNNNTGG	1 cut(s)	1124
AccBSI	CCGCTC	1 cut(s)	904
AccI	GTMKAC	1 cut(s)	1403
AccII	CGCG	1 cut(s)	221
AciI	CCGC	6 cut(s)	902, 1169, 1268, 1271, 1613, 1769
AclI	AACGTT	1 cut(s)	372
AclWI	GGATC	4 cut(s)	528, 645, 660, 2102
AcsI	RAATTY	6 cut(s)	568, 1259, 1426, 1541, 1931, 2192
AcuI	CTGAAG	4 cut(s)	1367, 1379, 1440, 1905
AfaI	GTAC	3 cut(s)	593, 1201, 1513
AfiI	CCNNNNNNNGG	8 cut(s)	155, 187, 563, 812, 839, 1124, 1341, 2022
AflIII	ACRYGT	1 cut(s)	627
AhlI	ACTAGT	3 cut(s)	961, 1552, 1597
AjiI	CACGTC	1 cut(s)	628
AjnI	CCWGG	1 cut(s)	409
AjuI	GAANNNNNNNTTGG	4 cut(s)	850, 882, 1416, 1448
AleI	CACNNNNGTG	1 cut(s)	1959
Alw21I	GWGCWC	5 cut(s)	805, 820, 1753, 1794, 1998
Alw26I	GTCTC	2 cut(s)	171, 1852
Alw44I	GTGCAC	2 cut(s)	801, 1790
AlwI	GGATC	4 cut(s)	528, 645, 660, 2102
AlwNI	CAGNNNCTG	1 cut(s)	655
AoxI	GGCC	4 cut(s)	683, 842, 1104, 2030
ApaI	GGGCCC	1 cut(s)	846
ApaLI	GTGCAC	2 cut(s)	801, 1790
ApeKI	GCWGC	3 cut(s)	110, 1521, 1742
ApoI	RAATTY	6 cut(s)	568, 1259, 1426, 1541, 1931, 2192
Asp700I	GAANNNNTTC	2 cut(s)	373, 1077
AspLEI	GCGC	1 cut(s)	1722
AspS9I	GGNCC	5 cut(s)	554, 683, 842, 843, 1325
AsuHPI	GGTGA	7 cut(s)	67, 284, 599, 753, 1688, 1946, 2051
AvaII	GGWCC	2 cut(s)	554, 1325
BaeGI	GKGCMC	3 cut(s)	805, 846, 1794
BanII	GRGCYC	3 cut(s)	846, 1753, 1998
BarI	GAAGNNNNNNTAC	2 cut(s)	58, 90
BauI	CACGAG	2 cut(s)	819, 2034
BbsI	GAAGAC	4 cut(s)	28, 66, 208, 1070
Bbv12I	GWGCWC	5 cut(s)	805, 820, 1753, 1794, 1998
BbvCI	CCTCAGC	1 cut(s)	1295
BbvI	GCAGC	3 cut(s)	97, 1533, 1754
BccI	CCATC	5 cut(s)	433, 766, 1118, 1153, 2095
BceAI	ACGGC	1 cut(s)	1643
BciT130I	CCWGG	1 cut(s)	411
BciVI	GTATCC	1 cut(s)	1300
BclI	TGATCA	1 cut(s)	1477
BcoDI	GTCTC	2 cut(s)	171, 1852
BcuI	ACTAGT	3 cut(s)	961, 1552, 1597
BfaI	CTAG	6 cut(s)	137, 551, 798, 962, 1553, 1598
BfmI	CTRYAG	5 cut(s)	111, 596, 647, 1035, 1969
BfuAI	ACCTGC	1 cut(s)	603
BfuI	GTATCC	1 cut(s)	1300
BisI	GCNGC	6 cut(s)	111, 902, 1268, 1271, 1522, 1743
BlpI	GCTNAGC	1 cut(s)	1467
BlsI	GCNGC	6 cut(s)	112, 903, 1269, 1272, 1523, 1744
BmcAI	AGTACT	1 cut(s)	1201
Bme1390I	CCNGG	1 cut(s)	411
Bme18I	GGWCC	2 cut(s)	554, 1325
BmgBI	CACGTC	1 cut(s)	628
BmgT120I	GGNCC	5 cut(s)	554, 683, 842, 843, 1325
BmiI	GGNNCC	3 cut(s)	619, 844, 1866
BmrFI	CCNGG	1 cut(s)	411
BmrI	ACTGGG	1 cut(s)	278
BmsI	GCATC	8 cut(s)	414, 431, 475, 1152, 1397, 1660, 1731, 2077
BmuI	ACTGGG	1 cut(s)	278
BpiI	GAAGAC	4 cut(s)	28, 66, 208, 1070
BpmI	CTGGAG	2 cut(s)	750, 834
Bpu10I	CCTNAGC	2 cut(s)	1295, 2070
Bpu1102I	GCTNAGC	1 cut(s)	1467
BpuEI	CTTGAG	2 cut(s)	1088, 1775
BsaI	GGTCTC	1 cut(s)	171
BsaJI	CCNNGG	1 cut(s)	409
BsaWI	WCCGGW	1 cut(s)	527
BsaXI	ACNNNNNCTCC	2 cut(s)	807, 837
Bsc4I	CCNNNNNNNGG	8 cut(s)	155, 187, 563, 812, 839, 1124, 1341, 2022
Bse1I	ACTGG	7 cut(s)	200, 273, 619, 779, 883, 1125, 1509
BseBI	CCWGG	1 cut(s)	411
BseDI	CCNNGG	1 cut(s)	409
BseGI	GGATG	6 cut(s)	405, 1129, 1258, 1722, 1949, 2106
BseLI	CCNNNNNNNGG	8 cut(s)	155, 187, 563, 812, 839, 1124, 1341, 2022
BseMII	CTCAG	3 cut(s)	446, 1309, 1863
BseNI	ACTGG	7 cut(s)	200, 273, 619, 779, 883, 1125, 1509
BseRI	GAGGAG	3 cut(s)	189, 1857, 1892
BseSI	GKGCMC	3 cut(s)	805, 846, 1794
BseXI	GCAGC	3 cut(s)	97, 1533, 1754
Bsh1236I	CGCG	1 cut(s)	221
BshFI	GGCC	4 cut(s)	685, 844, 1106, 2032
BsiHKAI	GWGCWC	5 cut(s)	805, 820, 1753, 1794, 1998
BsiSI	CCGG	2 cut(s)	528, 833
BslFI	GGGAC	1 cut(s)	1672
BslI	CCNNNNNNNGG	8 cut(s)	155, 187, 563, 812, 839, 1124, 1341, 2022
BsmAI	GTCTC	2 cut(s)	171, 1852
BsmFI	GGGAC	1 cut(s)	1672
BsnI	GGCC	4 cut(s)	685, 844, 1106, 2032
Bso31I	GGTCTC	1 cut(s)	171
Bsp120I	GGGCCC	1 cut(s)	842
Bsp1286I	GDGCHC	6 cut(s)	805, 820, 846, 1753, 1794, 1998
Bsp143I	GATC	5 cut(s)	520, 637, 652, 1477, 2107
Bsp1720I	GCTNAGC	1 cut(s)	1467
Bsp68I	TCGCGA	1 cut(s)	221
BspACI	CCGC	6 cut(s)	902, 1169, 1268, 1271, 1613, 1769
BspANI	GGCC	4 cut(s)	685, 844, 1106, 2032
BspCNI	CTCAG	3 cut(s)	445, 1308, 1862
BspFNI	CGCG	1 cut(s)	221
BspHI	TCATGA	1 cut(s)	2077
BspLI	GGNNCC	3 cut(s)	619, 844, 1866
BspMAI	CTGCAG	2 cut(s)	115, 600
BspMI	ACCTGC	1 cut(s)	603
BspPI	GGATC	4 cut(s)	528, 645, 660, 2102
BspTNI	GGTCTC	1 cut(s)	171
BsrBI	CCGCTC	1 cut(s)	904
BsrI	ACTGG	7 cut(s)	200, 273, 619, 779, 883, 1125, 1509
BssECI	CCNNGG	1 cut(s)	409
BssMI	GATC	5 cut(s)	520, 637, 652, 1477, 2107
BssNAI	GTATAC	1 cut(s)	1404
BssSI	CACGAG	2 cut(s)	819, 2034
Bst1107I	GTATAC	1 cut(s)	1404
Bst2BI	CACGAG	2 cut(s)	819, 2034
Bst2UI	CCWGG	1 cut(s)	411
Bst4CI	ACNGT	6 cut(s)	364, 1312, 1511, 1610, 1717, 1856
Bst6I	CTCTTC	2 cut(s)	156, 1343
BstC8I	GCNNGC	7 cut(s)	29, 1343, 1441, 1445, 1519, 1526, 1767
BstDEI	CTNAG	9 cut(s)	242, 432, 500, 897, 1295, 1467, 1849, 2070, 2241
BstENI	CCTNNNNNAGG	2 cut(s)	153, 2020
BstF5I	GGATG	6 cut(s)	405, 1129, 1258, 1722, 1949, 2106
BstFNI	CGCG	1 cut(s)	221
BstHHI	GCGC	1 cut(s)	1722
BstKTI	GATC	5 cut(s)	523, 640, 655, 1480, 2110
BstMAI	GTCTC	2 cut(s)	171, 1852
BstMBI	GATC	5 cut(s)	520, 637, 652, 1477, 2107
BstMWI	GCNNNNNNNGC	5 cut(s)	782, 1449, 1739, 1748, 1840
BstNI	CCWGG	1 cut(s)	411
BstNSI	RCATGY	6 cut(s)	31, 35, 1447, 1583, 1769, 1847
BstSCI	CCNGG	1 cut(s)	409
BstSFI	CTRYAG	5 cut(s)	111, 596, 647, 1035, 1969
BstSLI	GKGCMC	3 cut(s)	805, 846, 1794
BstUI	CGCG	1 cut(s)	221
BstV1I	GCAGC	3 cut(s)	97, 1533, 1754
BstV2I	GAAGAC	4 cut(s)	28, 66, 208, 1070
BstX2I	RGATCY	3 cut(s)	520, 637, 652
BstXI	CCANNNNNNTGG	1 cut(s)	470
BstYI	RGATCY	3 cut(s)	520, 637, 652
BstZ17I	GTATAC	1 cut(s)	1404
BsuI	GTATCC	1 cut(s)	1300
BsuRI	GGCC	4 cut(s)	685, 844, 1106, 2032
BtrI	CACGTC	1 cut(s)	628
BtsCI	GGATG	6 cut(s)	405, 1129, 1258, 1722, 1949, 2106
BtsI	GCAGTG	4 cut(s)	120, 1459, 1673, 1905
BtsIMutI	CAGTG	6 cut(s)	120, 475, 1118, 1459, 1673, 1905
BtuMI	TCGCGA	1 cut(s)	221
BveI	ACCTGC	1 cut(s)	603
Cac8I	GCNNGC	7 cut(s)	29, 1343, 1441, 1445, 1519, 1526, 1767
CaiI	CAGNNNCTG	1 cut(s)	655
CciI	TCATGA	1 cut(s)	2077
CfoI	GCGC	1 cut(s)	1722
Cfr13I	GGNCC	5 cut(s)	554, 683, 842, 843, 1325
Csp6I	GTAC	3 cut(s)	592, 1200, 1512
CviQI	GTAC	3 cut(s)	592, 1200, 1512
DdeI	CTNAG	9 cut(s)	242, 432, 500, 897, 1295, 1467, 1849, 2070, 2241
DpnI	GATC	5 cut(s)	522, 639, 654, 1479, 2109
DpnII	GATC	5 cut(s)	520, 637, 652, 1477, 2107
DrdI	GACNNNNNNGTC	1 cut(s)	292
DseDI	GACNNNNNNGTC	1 cut(s)	292
Eam1104I	CTCTTC	2 cut(s)	156, 1343
EarI	CTCTTC	2 cut(s)	156, 1343
Ecl136II	GAGCTC	2 cut(s)	1751, 1996
Eco147I	AGGCCT	1 cut(s)	2032
Eco24I	GRGCYC	3 cut(s)	846, 1753, 1998
Eco31I	GGTCTC	1 cut(s)	171
Eco47I	GGWCC	2 cut(s)	554, 1325
Eco53kI	GAGCTC	2 cut(s)	1751, 1996
Eco57I	CTGAAG	4 cut(s)	1367, 1379, 1440, 1905
EcoICRI	GAGCTC	2 cut(s)	1751, 1996
EcoNI	CCTNNNNNAGG	2 cut(s)	153, 2020
EcoO109I	RGGNCCY	1 cut(s)	843
EcoRI	GAATTC	1 cut(s)	2192
EcoRII	CCWGG	1 cut(s)	409
EcoT22I	ATGCAT	4 cut(s)	29, 33, 1218, 2092
EcoT38I	GRGCYC	3 cut(s)	846, 1753, 1998
FalI	AAGNNNNNCTT	2 cut(s)	147, 179
FaqI	GGGAC	1 cut(s)	1672
FbaI	TGATCA	1 cut(s)	1477
FblI	GTMKAC	1 cut(s)	1403
Fnu4HI	GCNGC	6 cut(s)	111, 902, 1268, 1271, 1522, 1743
FokI	GGATG	6 cut(s)	392, 1136, 1265, 1709, 1956, 2113
FriOI	GRGCYC	3 cut(s)	846, 1753, 1998
Fsp4HI	GCNGC	6 cut(s)	111, 902, 1268, 1271, 1522, 1743
FspAI	RTGCGCAY	1 cut(s)	1721
FspBI	CTAG	6 cut(s)	137, 551, 798, 962, 1553, 1598
FspI	TGCGCA	1 cut(s)	1721
GlaI	GCGC	1 cut(s)	1721
GluI	GCNGC	6 cut(s)	111, 902, 1268, 1271, 1522, 1743
GsuI	CTGGAG	2 cut(s)	750, 834
HaeIII	GGCC	4 cut(s)	685, 844, 1106, 2032
HapII	CCGG	2 cut(s)	528, 833
HhaI	GCGC	1 cut(s)	1722
Hin6I	GCGC	1 cut(s)	1720
HinP1I	GCGC	1 cut(s)	1720
HincII	GTYRAC	2 cut(s)	548, 1778
HindII	GTYRAC	2 cut(s)	548, 1778
HindIII	AAGCTT	6 cut(s)	239, 1028, 1469, 1484, 1832, 2072
HinfI	GANTC	6 cut(s)	58, 71, 829, 1351, 1660, 1937
HpaII	CCGG	2 cut(s)	528, 833
HphI	GGTGA	7 cut(s)	67, 284, 599, 753, 1688, 1946, 2051
Hpy166II	GTNNAC	6 cut(s)	548, 803, 959, 1404, 1778, 1792
Hpy8I	GTNNAC	6 cut(s)	548, 803, 959, 1404, 1778, 1792
HpyAV	CCTTC	3 cut(s)	816, 1067, 1297
HpyCH4III	ACNGT	6 cut(s)	364, 1312, 1511, 1610, 1717, 1856
HpyCH4IV	ACGT	3 cut(s)	372, 627, 2149
HpyF10VI	GCNNNNNNNGC	5 cut(s)	782, 1449, 1739, 1748, 1840
HpyF3I	CTNAG	9 cut(s)	242, 432, 500, 897, 1295, 1467, 1849, 2070, 2241
HpySE526I	ACGT	3 cut(s)	372, 627, 2149
HspAI	GCGC	1 cut(s)	1720
Ksp22I	TGATCA	1 cut(s)	1477
Kzo9I	GATC	5 cut(s)	520, 637, 652, 1477, 2107
LmnI	GCTCC	8 cut(s)	815, 1133, 1436, 1615, 1739, 1870, 2001, 2123
Lsp1109I	GCAGC	3 cut(s)	97, 1533, 1754
LweI	GCATC	8 cut(s)	414, 431, 475, 1152, 1397, 1660, 1731, 2077
MaeI	CTAG	6 cut(s)	137, 551, 798, 962, 1553, 1598
MaeII	ACGT	3 cut(s)	372, 627, 2149
MaeIII	GTNAC	4 cut(s)	226, 630, 986, 2145
MalI	GATC	5 cut(s)	522, 639, 654, 1479, 2109
MbiI	CCGCTC	1 cut(s)	904
MboI	GATC	5 cut(s)	520, 637, 652, 1477, 2107
MfeI	CAATTG	2 cut(s)	918, 1785
MflI	RGATCY	3 cut(s)	520, 637, 652
MhlI	GDGCHC	6 cut(s)	805, 820, 846, 1753, 1794, 1998
MlyI	GAGTC	3 cut(s)	80, 1360, 1654
MmeI	TCCRAC	3 cut(s)	151, 383, 2221
Mph1103I	ATGCAT	4 cut(s)	29, 33, 1218, 2092
MroXI	GAANNNNTTC	2 cut(s)	373, 1077
MseI	TTAA	4 cut(s)	132, 396, 539, 1236
MslI	CAYNNNNRTG	5 cut(s)	468, 1148, 1683, 1727, 1959
MspI	CCGG	2 cut(s)	528, 833
MspR9I	CCNGG	1 cut(s)	411
MunI	CAATTG	2 cut(s)	918, 1785
MvaI	CCWGG	1 cut(s)	411
MvnI	CGCG	1 cut(s)	221
MwoI	GCNNNNNNNGC	5 cut(s)	782, 1449, 1739, 1748, 1840
NdeII	GATC	5 cut(s)	520, 637, 652, 1477, 2107
NlaIV	GGNNCC	3 cut(s)	619, 844, 1866
NmuCI	GTSAC	2 cut(s)	986, 2145
NruI	TCGCGA	1 cut(s)	221
NsbI	TGCGCA	1 cut(s)	1721
NsiI	ATGCAT	4 cut(s)	29, 33, 1218, 2092
NspI	RCATGY	6 cut(s)	31, 35, 1447, 1583, 1769, 1847
OliI	CACNNNNGTG	1 cut(s)	1959
PaeI	GCATGC	3 cut(s)	31, 1447, 1769
PagI	TCATGA	1 cut(s)	2077
PceI	AGGCCT	1 cut(s)	2032
PdmI	GAANNNNTTC	2 cut(s)	373, 1077
PfeI	GAWTC	3 cut(s)	58, 829, 1937
PflMI	CCANNNNNTGG	1 cut(s)	1124
PkrI	GCNGC	6 cut(s)	112, 903, 1269, 1272, 1523, 1744
PleI	GAGTC	3 cut(s)	79, 1359, 1654
PpsI	GAGTC	3 cut(s)	79, 1359, 1654
Psp124BI	GAGCTC	2 cut(s)	1753, 1998
Psp1406I	AACGTT	1 cut(s)	372
Psp6I	CCWGG	1 cut(s)	409
PspGI	CCWGG	1 cut(s)	409
PspN4I	GGNNCC	3 cut(s)	619, 844, 1866
PspOMI	GGGCCC	1 cut(s)	842
PspPI	GGNCC	5 cut(s)	554, 683, 842, 843, 1325
PsrI	GAACNNNNNNTAC	2 cut(s)	1192, 1224
PstI	CTGCAG	2 cut(s)	115, 600
PstNI	CAGNNNCTG	1 cut(s)	655
PsuI	RGATCY	3 cut(s)	520, 637, 652
RruI	TCGCGA	1 cut(s)	221
RsaI	GTAC	3 cut(s)	593, 1201, 1513
RsaNI	GTAC	3 cut(s)	592, 1200, 1512
RseI	CAYNNNNRTG	5 cut(s)	468, 1148, 1683, 1727, 1959
SacI	GAGCTC	2 cut(s)	1753, 1998
SaqAI	TTAA	4 cut(s)	132, 396, 539, 1236
SatI	GCNGC	6 cut(s)	111, 902, 1268, 1271, 1522, 1743
Sau3AI	GATC	5 cut(s)	520, 637, 652, 1477, 2107
Sau96I	GGNCC	5 cut(s)	554, 683, 842, 843, 1325
ScaI	AGTACT	1 cut(s)	1201
SchI	GAGTC	3 cut(s)	80, 1360, 1654
ScrFI	CCNGG	1 cut(s)	411
SduI	GDGCHC	6 cut(s)	805, 820, 846, 1753, 1794, 1998
SfaNI	GCATC	8 cut(s)	414, 431, 475, 1152, 1397, 1660, 1731, 2077
SfcI	CTRYAG	5 cut(s)	111, 596, 647, 1035, 1969
SinI	GGWCC	2 cut(s)	554, 1325
SmiMI	CAYNNNNRTG	5 cut(s)	468, 1148, 1683, 1727, 1959
SmlI	CTYRAG	2 cut(s)	1067, 1754
SmoI	CTYRAG	2 cut(s)	1067, 1754
SpeI	ACTAGT	3 cut(s)	961, 1552, 1597
SphI	GCATGC	3 cut(s)	31, 1447, 1769
SseBI	AGGCCT	1 cut(s)	2032
SsiI	CCGC	6 cut(s)	902, 1169, 1268, 1271, 1613, 1769
SspI	AATATT	5 cut(s)	340, 661, 1588, 1693, 2048
SspMI	CTAG	6 cut(s)	137, 551, 798, 962, 1553, 1598
SstI	GAGCTC	2 cut(s)	1753, 1998
StuI	AGGCCT	1 cut(s)	2032
StyD4I	CCNGG	1 cut(s)	409
TaaI	ACNGT	6 cut(s)	364, 1312, 1511, 1610, 1717, 1856
TaiI	ACGT	3 cut(s)	375, 630, 2152
TaqI	TCGA	1 cut(s)	2182
TatI	WGTACW	2 cut(s)	1199, 1511
TauI	GCSGC	3 cut(s)	904, 1270, 1273
TfiI	GAWTC	3 cut(s)	58, 829, 1937
Tru1I	TTAA	4 cut(s)	132, 396, 539, 1236
Tru9I	TTAA	4 cut(s)	132, 396, 539, 1236
TscAI	CASTG	6 cut(s)	120, 475, 1125, 1459, 1680, 1912
TseFI	GTSAC	2 cut(s)	986, 2145
TseI	GCWGC	3 cut(s)	110, 1521, 1742
Tsp45I	GTSAC	2 cut(s)	986, 2145
TspDTI	ATGAA	6 cut(s)	777, 921, 1269, 1842, 2066, 2150
TspGWI	ACGGA	3 cut(s)	546, 1265, 1715
TspRI	CASTG	6 cut(s)	120, 475, 1125, 1459, 1680, 1912
Van91I	CCANNNNNTGG	1 cut(s)	1124
VneI	GTGCAC	2 cut(s)	801, 1790
VpaK11BI	GGWCC	2 cut(s)	554, 1325
XagI	CCTNNNNNAGG	2 cut(s)	153, 2020
XapI	RAATTY	6 cut(s)	568, 1259, 1426, 1541, 1931, 2192
XceI	RCATGY	6 cut(s)	31, 35, 1447, 1583, 1769, 1847
XmiI	GTMKAC	1 cut(s)	1403
XmnI	GAANNNNTTC	2 cut(s)	373, 1077
XspI	CTAG	6 cut(s)	137, 551, 798, 962, 1553, 1598
ZrmI	AGTACT	1 cut(s)	1201
Zsp2I	ATGCAT	4 cut(s)	29, 33, 1218, 2092

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.