Rw6G026840

zinc finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0015546
Physical Location & Seq
Reverse (-)
50375089 .. 50375996
908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G026840.1

Sequence Viewer

Length: 630 bp
ATGGAATCTCAATCTAACCATCGTCACGATGAAGAAGATTCGAAGAGCTCTAGCGATGAAGAAGCCGAGGATCTCAACGATGACTTGGGAACAGGGCGATCATATGAGTGTGTGTTTTGCAAGAGAGGATTCACAACAGCACAGGCCTTGGGTGGACACATGAATATTCACCGCAAGGAGAGAGCCAAGCCGAGGCCTACTTCTGCTCCTACTTCAATTGCAAGCAAAGCAGCTGATGAACACGTGAGTGATCTCATGAGATCCTACCGTTCGTTGCAAAGCTACACGGGTCCACATTTTGCCACAGCTCCGGAGGTGGATGTGAATTACCGGACGTATATTCCGGCACCTACGTGGATTGTGGGACAAGCTCACAGTGATCATGAGGAGTTATGTGCGAGGAATTATCAGCTACGGCATCAGAATTTGTTTGCTACAGATGATGATTGTCGGAATTGGAGATCGGGGAGTGGTCTGAGCTTGGGAATTGGGCCATCAGCTCATGATGATGATGATGAGGATCATGTAGGTCATGATCATCATAAAAAAGAAATTGAACAAATTAATGGTGCCTGCAGTAGTGAAGGGGATGGATTGGATTTGGAGCTTCGACTCGGTCATGATCCTTAG

Protein Analysis

209

Amino Acids

23.28

Weight (kDa)

5.11

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_6 PF13912 35 - 60 1e-05 C2H2-type zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015546)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g22030
malus_domestica MD05G1201300.v1.1 MD10G1188100.v1.1
prunus_persica Prupe.8G227900_v2.0.a1
pyrus_communis pycom05g18810 pycom10g16260
rosa_chinensis RchiOBHm_Chr6g0288891
rosa_laevigata RLG00000012352
rosa_multiflora Rmu_sc0005314.1_g000005
rosa_roxburghii Rroxscaffold_7G00179790
rosa_rugosa Rorug06G0199000
rosa_samantha Rh6AG310500 Rh6BG317000 Rh6CG323700 Rh6DG309400
rosa_wichuraiana Rw6G026840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 346, 569
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 1 cut(s) 172
AclWI GGATC 4 cut(s) 78, 255, 528, 617
AcsI RAATTY 1 cut(s) 424
AcvI CACGTG 1 cut(s) 244
AfiI CCNNNNNNNGG 1 cut(s) 192
AflIII ACRYGT 1 cut(s) 241
AgsI TTSAA 2 cut(s) 216, 557
AleI CACNNNNGTG 2 cut(s) 246, 352
AluBI AGCT 9 cut(s) 48, 233, 282, 308, 371, 412, 480, 500, 607
AluI AGCT 9 cut(s) 48, 233, 282, 308, 371, 412, 480, 500, 607
Alw21I GWGCWC 1 cut(s) 50
AlwI GGATC 4 cut(s) 78, 255, 528, 617
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 3 cut(s) 144, 194, 491
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 1 cut(s) 424
AseI ATTAAT 1 cut(s) 564
AspS9I GGNCC 2 cut(s) 290, 491
AsuHPI GGTGA 1 cut(s) 161
AsuII TTCGAA 1 cut(s) 41
AvaII GGWCC 1 cut(s) 290
BanI GGYRCC 2 cut(s) 346, 569
BanII GRGCYC 1 cut(s) 50
BbrPI CACGTG 1 cut(s) 244
Bbv12I GWGCWC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 242
BccI CCATC 3 cut(s) 27, 502, 584
BceAI ACGGC 1 cut(s) 431
BclI TGATCA 2 cut(s) 379, 535
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 2 cut(s) 435, 574
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 290
BmgT120I GGNCC 2 cut(s) 290, 491
BmiI GGNNCC 3 cut(s) 291, 348, 571
BmsI GCATC 1 cut(s) 427
Bpu14I TTCGAA 1 cut(s) 41
BsaAI YACGTR 2 cut(s) 244, 354
BsaBI GATNNNNATC 1 cut(s) 519
BsaJI CCNNGG 3 cut(s) 66, 147, 191
BsaWI WCCGGW 2 cut(s) 310, 330
BsaXI ACNNNNNCTCC 2 cut(s) 190, 220
Bsc4I CCNNNNNNNGG 1 cut(s) 192
Bse8I GATNNNNATC 1 cut(s) 519
BseAI TCCGGA 1 cut(s) 310
BseDI CCNNGG 3 cut(s) 66, 147, 191
BseGI GGATG 2 cut(s) 325, 595
BseJI GATNNNNATC 1 cut(s) 519
BseLI CCNNNNNNNGG 1 cut(s) 192
BseMII CTCAG 1 cut(s) 467
BseRI GAGGAG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 3 cut(s) 146, 196, 493
BshNI GGYRCC 2 cut(s) 346, 569
BsiHKAI GWGCWC 1 cut(s) 50
BsiSI CCGG 3 cut(s) 311, 331, 344
BslFI GGGAC 1 cut(s) 378
BslI CCNNNNNNNGG 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 378
BsnI GGCC 3 cut(s) 146, 196, 493
Bsp119I TTCGAA 1 cut(s) 41
Bsp1286I GDGCHC 1 cut(s) 50
Bsp13I TCCGGA 1 cut(s) 310
Bsp143I GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
BspACI CCGC 1 cut(s) 172
BspANI GGCC 3 cut(s) 146, 196, 493
BspCNI CTCAG 1 cut(s) 468
BspEI TCCGGA 1 cut(s) 310
BspHI TCATGA 5 cut(s) 255, 382, 502, 532, 619
BspLI GGNNCC 3 cut(s) 291, 348, 571
BspMAI CTGCAG 1 cut(s) 578
BspPI GGATC 4 cut(s) 78, 255, 528, 617
BspQI GCTCTTC 1 cut(s) 38
BspT104I TTCGAA 1 cut(s) 41
BspT107I GGYRCC 2 cut(s) 346, 569
BssECI CCNNGG 3 cut(s) 66, 147, 191
BssMI GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
BssT1I CCWWGG 1 cut(s) 147
Bst4CI ACNGT 2 cut(s) 269, 377
Bst6I CTCTTC 1 cut(s) 38
BstBAI YACGTR 2 cut(s) 244, 354
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 2 cut(s) 223, 574
BstDEI CTNAG 2 cut(s) 476, 627
BstF5I GGATG 2 cut(s) 325, 595
BstKTI GATC 9 cut(s) 73, 101, 253, 263, 382, 464, 523, 538, 625
BstMBI GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
BstMWI GCNNNNNNNGC 1 cut(s) 227
BstSFI CTRYAG 2 cut(s) 435, 574
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 2 cut(s) 70, 260
BstYI RGATCY 2 cut(s) 70, 260
BsuRI GGCC 3 cut(s) 146, 196, 493
BtgZI GCGATG 1 cut(s) 69
BtsCI GGATG 2 cut(s) 325, 595
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 2 cut(s) 223, 574
CciI TCATGA 5 cut(s) 255, 382, 502, 532, 619
Cfr13I GGNCC 2 cut(s) 290, 491
CviAII CATG 7 cut(s) 160, 256, 383, 503, 524, 533, 620
DdeI CTNAG 2 cut(s) 476, 627
DpnI GATC 9 cut(s) 72, 100, 252, 262, 381, 463, 522, 537, 624
DpnII GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 48
Eco130I CCWWGG 1 cut(s) 147
Eco147I AGGCCT 2 cut(s) 146, 196
Eco24I GRGCYC 1 cut(s) 50
Eco47I GGWCC 1 cut(s) 290
Eco53kI GAGCTC 1 cut(s) 48
Eco72I CACGTG 1 cut(s) 244
EcoICRI GAGCTC 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 147
EcoT38I GRGCYC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 7 cut(s) 163, 259, 386, 506, 527, 536, 623
FaqI GGGAC 1 cut(s) 378
FatI CATG 7 cut(s) 159, 255, 382, 502, 523, 532, 619
FauNDI CATATG 1 cut(s) 103
FbaI TGATCA 2 cut(s) 379, 535
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 2 cut(s) 332, 602
FriOI GRGCYC 1 cut(s) 50
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 51
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 3 cut(s) 146, 196, 493
HapII CCGG 3 cut(s) 311, 331, 344
Hin1II CATG 7 cut(s) 163, 259, 386, 506, 527, 536, 623
HinfI GANTC 4 cut(s) 5, 38, 129, 612
HpaII CCGG 3 cut(s) 311, 331, 344
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 155, 293
Hpy188I TCNGA 3 cut(s) 423, 453, 477
Hpy188III TCNNGA 7 cut(s) 26, 256, 311, 383, 503, 533, 620
Hpy8I GTNNAC 2 cut(s) 155, 293
HpyAV CCTTC 1 cut(s) 578
HpyCH4III ACNGT 2 cut(s) 269, 377
HpyCH4IV ACGT 3 cut(s) 243, 335, 353
HpyCH4V TGCA 4 cut(s) 120, 221, 277, 576
HpyF10VI GCNNNNNNNGC 1 cut(s) 227
HpyF3I CTNAG 2 cut(s) 476, 627
HpySE526I ACGT 3 cut(s) 243, 335, 353
Hsp92II CATG 7 cut(s) 163, 259, 386, 506, 527, 536, 623
Kpn2I TCCGGA 1 cut(s) 310
Ksp22I TGATCA 2 cut(s) 379, 535
Kzo9I GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
LguI GCTCTTC 1 cut(s) 38
LmnI GCTCC 3 cut(s) 211, 313, 604
LpnPI CCDG 6 cut(s) 78, 128, 324, 344, 357, 586
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 427
MaeI CTAG 1 cut(s) 51
MaeII ACGT 3 cut(s) 243, 335, 353
MaeIII GTNAC 1 cut(s) 23
MalI GATC 9 cut(s) 72, 100, 252, 262, 381, 463, 522, 537, 624
MboI GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
MboII GAAGA 4 cut(s) 44, 47, 55, 71
MfeI CAATTG 1 cut(s) 216
MflI RGATCY 2 cut(s) 70, 260
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 8 cut(s) 216, 325, 403, 424, 454, 486, 552, 561
MlyI GAGTC 1 cut(s) 606
MmeI TCCRAC 1 cut(s) 431
MnlI CCTC 7 cut(s) 61, 119, 186, 307, 379, 393, 511
MroI TCCGGA 1 cut(s) 310
MseI TTAA 1 cut(s) 564
MslI CAYNNNNRTG 4 cut(s) 106, 246, 352, 507
MspA1I CMGCKG 1 cut(s) 233
MspI CCGG 3 cut(s) 311, 331, 344
MunI CAATTG 1 cut(s) 216
MwoI GCNNNNNNNGC 1 cut(s) 227
NdeI CATATG 1 cut(s) 103
NdeII GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
NlaIII CATG 7 cut(s) 163, 259, 386, 506, 527, 536, 623
NlaIV GGNNCC 3 cut(s) 291, 348, 571
NmeAIII GCCGAG 2 cut(s) 91, 216
NmuCI GTSAC 1 cut(s) 23
NspV TTCGAA 1 cut(s) 41
OliI CACNNNNGTG 2 cut(s) 246, 352
PagI TCATGA 5 cut(s) 255, 382, 502, 532, 619
PceI AGGCCT 2 cut(s) 146, 196
PciSI GCTCTTC 1 cut(s) 38
PfeI GAWTC 3 cut(s) 5, 38, 129
PflFI GACNNNGTC 1 cut(s) 615
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 1 cut(s) 606
PmaCI CACGTG 1 cut(s) 244
PmlI CACGTG 1 cut(s) 244
PpsI GAGTC 1 cut(s) 606
Ppu21I YACGTR 2 cut(s) 244, 354
PshBI ATTAAT 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 50
PspCI CACGTG 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 291, 348, 571
PspPI GGNCC 2 cut(s) 290, 491
PstI CTGCAG 1 cut(s) 578
PsuI RGATCY 2 cut(s) 70, 260
PsyI GACNNNGTC 1 cut(s) 615
PvuII CAGCTG 1 cut(s) 233
RseI CAYNNNNRTG 4 cut(s) 106, 246, 352, 507
SacI GAGCTC 1 cut(s) 50
SapI GCTCTTC 1 cut(s) 38
SaqAI TTAA 1 cut(s) 564
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 9 cut(s) 70, 98, 250, 260, 379, 461, 520, 535, 622
Sau96I GGNCC 2 cut(s) 290, 491
SchI GAGTC 1 cut(s) 606
SduI GDGCHC 1 cut(s) 50
SfaNI GCATC 1 cut(s) 427
SfcI CTRYAG 2 cut(s) 435, 574
SfuI TTCGAA 1 cut(s) 41
SinI GGWCC 1 cut(s) 290
SmiMI CAYNNNNRTG 4 cut(s) 106, 246, 352, 507
Sse9I AATT 8 cut(s) 216, 325, 403, 424, 454, 486, 552, 561
SseBI AGGCCT 2 cut(s) 146, 196
SsiI CCGC 1 cut(s) 172
SspI AATATT 1 cut(s) 166
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 50
StuI AGGCCT 2 cut(s) 146, 196
StyI CCWWGG 1 cut(s) 147
TaaI ACNGT 2 cut(s) 269, 377
TaiI ACGT 3 cut(s) 246, 338, 356
TaqI TCGA 2 cut(s) 41, 610
TaqII GACCGA 1 cut(s) 605
TasI AATT 8 cut(s) 216, 325, 403, 424, 454, 486, 552, 561
TfiI GAWTC 3 cut(s) 5, 38, 129
Tru1I TTAA 1 cut(s) 564
Tru9I TTAA 1 cut(s) 564
TscAI CASTG 1 cut(s) 382
TseFI GTSAC 1 cut(s) 23
TseI GCWGC 1 cut(s) 230
Tsp45I GTSAC 1 cut(s) 23
TspDTI ATGAA 4 cut(s) 45, 72, 176, 252
TspRI CASTG 1 cut(s) 382
Tth111I GACNNNGTC 1 cut(s) 615
VpaK11BI GGWCC 1 cut(s) 290
VspI ATTAAT 1 cut(s) 564
XapI RAATTY 1 cut(s) 424
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.