Rw7G021860
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0018898
Physical Location & Seq
Reverse (-)
25474127 .. 25475201
1075 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G021860.1

Sequence Viewer

Length: 417 bp
ATGAAGTTCTCCGCCATAGAGATATATAATGAAGCTGTTGGAGATCTTCTTAGCTCCGAGAATGCTCAACTTAGGCTGCTAGATGATCCAGAGAATTTTGTTGATCTTGTGGGCAGCGAGCGTGTCGCTCAGGCATTATCAGCTGGTGCAAGATTGAAAGAAGGTTGTCACATAAATAGAAGTGTACTGACCCTGGGAACTGTAATACGCAAATTAAGTCATGTTGAGCAATCAAACAACACCCTGTTCTTTGCAAGTTGTGCCAAAGAGGTTACTACTAATGCAATGGTCAATGTGGTAAGGTCAGATAAGGCATTGGTAAAGCAATATCATAATGTGTGTATATGTGCATATTGCCATATGAGTTATTTCTGTGAAGGTTCCGTAATAGCAATTCTTACTGCATGTGAGAGCTAA

Protein Analysis

138

Amino Acids

15.12

Weight (kDa)

5.76

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Kinesin PF00225 32 - 75 4.4e-11 Kinesin motor domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0018898)

Species Orthologous Gene IDs
malus_domestica MD02G1101600.v1.1 MD02G1102000.v1.1
pyrus_communis pycom02g07970
rosa_multiflora Rmu_sc0010835.1_g000003 Rmu_sc0021237.1_g000001
rosa_roxburghii Rroxscaffold_2G00087080 Rroxscaffold_3G00267430 Rroxscaffold_6G00414250
rosa_wichuraiana Rw7G021860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 12
AclWI GGATC 1 cut(s) 80
AcsI RAATTY 1 cut(s) 94
AfaI GTAC 1 cut(s) 186
AgsI TTSAA 1 cut(s) 157
AjnI CCWGG 1 cut(s) 192
AluBI AGCT 4 cut(s) 35, 54, 143, 414
AluI AGCT 4 cut(s) 35, 54, 143, 414
AlwI GGATC 1 cut(s) 80
ApeKI GCWGC 2 cut(s) 76, 114
ApoI RAATTY 1 cut(s) 94
BbvI GCAGC 2 cut(s) 63, 126
BciT130I CCWGG 1 cut(s) 194
BfaI CTAG 1 cut(s) 80
BglII AGATCT 1 cut(s) 43
BisI GCNGC 2 cut(s) 77, 115
BlsI GCNGC 2 cut(s) 78, 116
Bme1390I CCNGG 1 cut(s) 194
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 192, 193
Bse3DI GCAATG 1 cut(s) 291
BseBI CCWGG 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 192, 193
BseMI GCAATG 1 cut(s) 291
BseMII CTCAG 1 cut(s) 143
BseXI GCAGC 2 cut(s) 63, 126
BsmI GAATGC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 43, 85, 103
BspACI CCGC 1 cut(s) 12
BspCNI CTCAG 1 cut(s) 142
BspLI GGNNCC 1 cut(s) 382
BspPI GGATC 1 cut(s) 80
BsrDI GCAATG 1 cut(s) 291
BssECI CCNNGG 2 cut(s) 192, 193
BssMI GATC 3 cut(s) 43, 85, 103
Bst2UI CCWGG 1 cut(s) 194
Bst4CI ACNGT 1 cut(s) 202
BstAPI GCANNNNNTGC 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 3 cut(s) 50, 71, 129
BstKTI GATC 3 cut(s) 46, 88, 106
BstMBI GATC 3 cut(s) 43, 85, 103
BstMWI GCNNNNNNNGC 2 cut(s) 140, 260
BstNI CCWGG 1 cut(s) 194
BstNSI RCATGY 1 cut(s) 408
BstSCI CCNGG 1 cut(s) 192
BstV1I GCAGC 2 cut(s) 63, 126
BstX2I RGATCY 1 cut(s) 43
BstYI RGATCY 1 cut(s) 43
Cac8I GCNNGC 1 cut(s) 119
Csp6I GTAC 1 cut(s) 185
CviAII CATG 2 cut(s) 221, 405
CviJI RGCY 5 cut(s) 35, 54, 76, 143, 414
CviKI_1 RGCY 5 cut(s) 35, 54, 76, 143, 414
CviQI GTAC 1 cut(s) 185
DdeI CTNAG 3 cut(s) 50, 71, 129
DpnI GATC 3 cut(s) 45, 87, 105
DpnII GATC 3 cut(s) 43, 85, 103
EcoRII CCWGG 1 cut(s) 192
FaeI CATG 2 cut(s) 224, 408
FatI CATG 2 cut(s) 220, 404
FauNDI CATATG 1 cut(s) 360
Fnu4HI GCNGC 2 cut(s) 77, 115
Fsp4HI GCNGC 2 cut(s) 77, 115
FspBI CTAG 1 cut(s) 80
GluI GCNGC 2 cut(s) 77, 115
Hin1II CATG 2 cut(s) 224, 408
Hpy166II GTNNAC 1 cut(s) 185
Hpy188I TCNGA 2 cut(s) 58, 307
Hpy188III TCNNGA 1 cut(s) 89
Hpy8I GTNNAC 1 cut(s) 185
HpyAV CCTTC 2 cut(s) 155, 371
HpyCH4III ACNGT 1 cut(s) 202
HpyCH4V TGCA 5 cut(s) 149, 254, 284, 350, 404
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 260
HpyF3I CTNAG 3 cut(s) 50, 71, 129
Hsp92II CATG 2 cut(s) 224, 408
Kzo9I GATC 3 cut(s) 43, 85, 103
LmnI GCTCC 1 cut(s) 59
LpnPI CCDG 6 cut(s) 102, 116, 129, 179, 206, 257
Lsp1109I GCAGC 2 cut(s) 63, 126
MaeI CTAG 1 cut(s) 80
MaeIII GTNAC 2 cut(s) 167, 271
MalI GATC 3 cut(s) 45, 87, 105
MboI GATC 3 cut(s) 43, 85, 103
MboII GAAGA 1 cut(s) 38
MflI RGATCY 1 cut(s) 43
MluCI AATT 3 cut(s) 94, 212, 393
MmeI TCCRAC 1 cut(s) 19
MnlI CCTC 1 cut(s) 262
MseI TTAA 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 1 cut(s) 194
Mva1269I GAATGC 1 cut(s) 67
MvaI CCWGG 1 cut(s) 194
MwoI GCNNNNNNNGC 2 cut(s) 140, 260
NdeI CATATG 1 cut(s) 360
NdeII GATC 3 cut(s) 43, 85, 103
NlaIII CATG 2 cut(s) 224, 408
NlaIV GGNNCC 1 cut(s) 382
NmuCI GTSAC 1 cut(s) 167
NspI RCATGY 1 cut(s) 408
PasI CCCWGGG 1 cut(s) 193
PctI GAATGC 1 cut(s) 67
PkrI GCNGC 2 cut(s) 78, 116
Psp6I CCWGG 1 cut(s) 192
PspGI CCWGG 1 cut(s) 192
PspN4I GGNNCC 1 cut(s) 382
PsuI RGATCY 1 cut(s) 43
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 186
RsaNI GTAC 1 cut(s) 185
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 2 cut(s) 77, 115
Sau3AI GATC 3 cut(s) 43, 85, 103
ScrFI CCNGG 1 cut(s) 194
SetI ASST 8 cut(s) 37, 56, 145, 166, 273, 305, 382, 416
Sse9I AATT 3 cut(s) 94, 212, 393
SsiI CCGC 1 cut(s) 12
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 192
TaaI ACNGT 1 cut(s) 202
TasI AATT 3 cut(s) 94, 212, 393
TatI WGTACW 1 cut(s) 184
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TseFI GTSAC 1 cut(s) 167
TseI GCWGC 2 cut(s) 76, 114
Tsp45I GTSAC 1 cut(s) 167
TspDTI ATGAA 2 cut(s) 17, 45
TspGWI ACGGA 1 cut(s) 373
XapI RAATTY 1 cut(s) 94
XceI RCATGY 1 cut(s) 408
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.