Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr7

Orthogroup ID

OG0018287

Physical Location & Seq

Genomic Coordinates Reverse (-)

65958956 .. 65967626

Gene Structure 8671 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw7G042330.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3816 bp

ATGTCACGTCGTCGAAGCAACGGCGCATCCCCATTGGTTAATCCACAGCGCCAAATCACTTCCTTCTTCGCCAAAGCCACTTCCTCTCCATCTCCAATCCCTAAATCTAAACCTAAACCCAGCCCGGGTTCCAGCCCTAGCTCCACCACGCCTTCGCCTCTCCAATCCAAGCCTAAGCCCAATCCCGATCCCGACCCCAATCCGAAGAAATTGTTGTACGGCCAAGAGGTGGTCGGCAAGAGGATCAAGGTGTACTGGGCGAGTGATCGGGCCTGGTACGAAGGCTGTGTCAAGTTGTTCACTAAGGAGAGTGGCAAGCACTTACTTCAGTACGACGATGGCGACGAAGAGACTTTGGATTTGGCCACCGAGAAAATCGAGTGGATTCAAGAGACCGTCCAGACCTTTAAGCGCTTGCGCCGCGGTCCTTTGCCCACTCCCGAGGCGCAGGAGGAGGAGGTCAAGGAAGACAGTGATGATGATGATGATGATTCCGGCGATGAGGATTGGGGAAAGAGTGTGGAAAAGGAACTAGTTGAGGAGGAGGAGGAGGAAGACGAGGTTATGGAATTGGAGGAGGAGCAAGATGATGATGATGATGATGAGGGACTCCAGCCCAAGCCCAAAGCAAGGCGCGGCGGCTCCGGTGGTAAACGGAAGTTGAGCGGCGGAGGGAATTTGGGGTCTGCTAAGAAGACCAAGACTGGTGGACATGTAGCCACTAATGGCCTCAAGCCTAAAGGGATTCAGCCTGCTACTACTACTACTACTACTAATGCTGCTGACAGCACAAAGGCGGTTAATGGTATCAATATTGGTGATGCCTCAGAAAGGTTTAGCAAGCGTGAAGCTGACAAGTTCCGTTTCCTCGGGGAAAAACGCAGGGATGCCAAAAAAAGACAACTTGGGGATGCAAATTATGATCCAAGAACTCTATATCTACCTCCTGATTTCTTAAAGAGTTTATCAGGTGGCCAGAGACAATGGTGGGAGTTTAAGTCAAAGCACATGGACAAAGTTCTATTTTTCAAGATGGGCAAGTTCTATGAACTTTTTGAAATGGATGCACATATAGGAGCAAAAGAACTTGATCTTCAATATATGAAGGGTGAACAACCCCATTGTGGATTTCCAGAGAAGAAATTTTCAATGCATGTGGAGAAACTGGCTAGGAAGGGTTACCGAGTTCTTGTCATAGAGCAGACAGAAACACCTGAACAGATGGAGCTTCGTCGCAAAGAGGGTGGCTCTAAAGATAAGGTTGTGAAACGTGAAGTATGTGCAGTAGTTACAAAAGGAACACTAACTGAGGGAGAGATGCTGTCCGCAAACCCTGATGCTTCTTATCTAATGGCAGTGACTGAAACCTCTCAAAATTTAGCAAACCAAAATGGCGAGCGTATTTTTGGAGTTTGTGTGGTTGATGTTGCTACCAGTAGGGTTATTCTTGGACAGTTCGCAGATGATTTAGAGTGCAGTGCCTTGTCTTGTCTCTTGTCTGAATTGAGGCCCGTGGAACTAGTAAAACCAGCAGAACTACTTAGTCCTGAGACGGAAAAAGTACTGCTACGACATACAAGAAGTCCACTAGTGAATGAGTTGGTTCCACTTCTGGAATTCTGGGATGCTGAGAGAACTGTTTGTGAAGTTAAAAGTATCTATAGCTGTGCTGATGATTCTCATATGGAAGAACATGGCTTGGGCTGCCTGCCAGATGTATTGTCTGAGCTTATGGGAGCCGGGGAGGATGGCATCTGTGCACTTTCAGCTCTTGGGGGTGTTCTTTTCTACTTAAAGCAGGCTTTTCTAGAAGATACATTGCTTAGATTTGCAAAATTTGAGTTACTTCCAAGTTCTGGTTTTGGTGGTATCATATCAAAACCATACATGGTTCTCGATGCAGCTGCACTAGAGAACCTGGAGATTTTTGAAAACAGCAGAAATGGAGACTCTTCAGGGACAATGTATGCACAATTGAATCACTGTGTGACGGCATTTGGGAAGAGATTGCTTAAGACATGGCTTGCGAGACCTCTGTATCATGTAGAGTCAATTAAGGAACGCCAGGATGCTGTCTCAAGTTTACAGGGAATCAACCTTCCTCACGCACTTGAATTTCGAAAAGCAATGGCTAGGCTTCCGGACATGGAGCGCTTGCTTGCACGGGTCTTCGCTAGCAGTAAAGCTACTGGAAGGAATGCAAACAAAGTGGTTTTATATGAGGATGCCGCCAAAAAACAGCTCCAGGAGTTTATTACAGCTCTACGCGGTTGTGAATTAATGGCGGCAACAAGTTGCTCTCTTGGAGTCAACTTGGGAAATGTTGAATCTCGACAACTTCATCATTTGCTGACACCTGGTAAAGGTCTTCCTAATGTCAACTCAGTTTTAAAGCACTTCAAGGATGGCTTTGATTGGGTAGAAGCCAATAGTTCAGGTCGTATAATACCTCATGAAGGAGTTGATACTGAATACGACGCTGCATGTGGAAAAGTAAAAGAGATCGAGTCTCATTTAATCAAGTACCTTAACGAACAGAGGGAATTACTCGGAGATAAATCGATCACTTATGTCACAATTGGAAAAGACGCATACCTATTGGAAGTTCCAGAAAGTTTGCGTGAGAGTATAACTCAGGATTATGAATTACGTTCATCCAAAAAGGGTTTCTTTAGGTACTGGACACCAAATATTAAGAAGTCATTGACTGAGCTCTCACAAGCTGAATCTGATAGGGAGTCCTCACTTAAAAGCATTTTGCAGAGGTTAATTGGACAATTCTGCGAGCATCATATTAAGTGGAGACAGTTGGTCTCTGTGACTGCTGAACTGGATGTTTTAATCAGTCTAGCAATTGCAAGTGATTATTACGAGGGACCCACCTGTCGGCCTGTTATCATGAGTTCATCAGATACAGATGAAGTACCCCTCTTGTCCGCAAATAGTTTGGGACATCCTGTTCTTAGAAGTGATTCTTTAGGAAAAGGTACATTTGTCCCCAATGACATTACTCTTGGAGGTTCTGGTCATGCAAGCTTTGTCCTTCTCACTGGCCCTAACATGGGTGGGAAGTCAACTCTGCTTCGCCAAGTTTGCTTGGCTGTGATTTTAGCCCAGTTAGGAGCAAATGTGCCTGCAGAGAGCTTTGAGCTATCTCCTGTTGATCGAATCTTTGTTCGGATGGGTGCTAAAGATCATATTATGGTAGGCCAAAGTACATTTCTGACGGAGCTGTCAGAAACTGCAACAATGCTGTCATCTGCTTCTCGTAACTCATTGGTAGCACTGGATGAACTTGGACGTGGGACATCAACTTCAGATGGACAAGCCATTGCGGAATCAGTTCTTGAACATTTTGTCCACAAGGTTCACTGTCGAGGAATGTTTTCTACTCACTATCACAGGCTAGCTGTTGATTATCAGAATAATTCTCAGGTTTCACTCTGCCATATGGCATGCCATGTTGGAAATGGAGATGCTGGTGTAGAAGAAGTGACATTTCTTTACAGATTGACTCCCGGTGCTTGTCCTAAAAGTTATGGTGTCAACATTGCTCGGTTAGCTGGACTTCCTATTTCTGTGCTTCAAAAAGCTGCTGCTAAGTCCAGGGAATTCGAAGCTGCATATGGTAAACACTTGAAGCAATCTGAAGATAGCTTTCCCTTTCAAAGTCCAGTTAACAAAGTGGTTGAGTGCATCCAAAAGGTTACCAACATCGTGGCAAAGTTGACTAGTCACGAGTCGACTGAGGGCATCAACATTGACTCCCTGACTGAAGTTTGGCATAAAGCAAGATTGCTTGAGCAACAAAGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000217 GO:0000404 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0003690 GO:0003824 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006259 GO:0006281 GO:0006290 GO:0006298 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008094 GO:0008150 GO:0008152 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0030983 GO:0032135 GO:0032300 GO:0032991 GO:0033554 GO:0034641 GO:0042623 GO:0043170 GO:0043570 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:1901360 GO:1901363 GO:1990391

KEGG Pathways

Metabolic & Signaling

ko01524 ko03430 ko05200 ko05210 map01524 map03430 map05200 map05210

Pfam Domains

Protein Families

MutS_I MutS_II MutS_III MutS_IV MutS_V

Protein Analysis

1271

Amino Acids

140.55

Weight (kDa)

5.77

Isoelectric Point (pI)

50.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MutS_I	PF01624	325 - 438	1.2e-33	MutS domain I
MutS_II	PF05188	448 - 531	1.8e-08	MutS domain II
MutS_III	PF05192	635 - 951	1.4e-34	MutS domain III
MutS_IV	PF05190	820 - 910	1.8e-14	MutS family domain IV
MutS_V	PF00488	1015 - 1205	3.9e-67	MutS domain V

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0018287)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G10950
rosa_chinensis	RchiOBHm_Chr7g0241701
rosa_multiflora	Rmu_sc0002268.1_g000005 Rmu_sc0017195.1_g000001
rosa_roxburghii	Rroxscaffold_1G00054410
rosa_rugosa	Rorug07G0346500
rosa_samantha	Rh2BG003900 Rh7AG501000 Rh7BG472200
rosa_wichuraiana	Rw7G042330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	2883, 3232
AccB7I	CCANNNNNTGG	2 cut(s)	229, 1856
AccBSI	CCGCTC	1 cut(s)	666
AccI	GTMKAC	1 cut(s)	3743
AccII	CGCG	3 cut(s)	423, 636, 2268
AccIII	TCCGGA	1 cut(s)	2140
AclWI	GGATC	3 cut(s)	182, 251, 917
AcoI	YGGCCR	3 cut(s)	220, 363, 973
AcsI	RAATTY	7 cut(s)	676, 1142, 1375, 1616, 1835, 2114, 3611
AcuI	CTGAAG	5 cut(s)	311, 1938, 3300, 3669, 3795
AdeI	CACNNNGTG	1 cut(s)	1987
AfeI	AGCGCT	2 cut(s)	413, 2153
AflII	CTTAAG	1 cut(s)	2012
AflIII	ACRYGT	1 cut(s)	712
AhdI	GACNNNNNGTC	1 cut(s)	2810
AhlI	ACTAGT	4 cut(s)	532, 1519, 1588, 3731
AjiI	CACGTC	2 cut(s)	8, 3302
AjnI	CCWGG	6 cut(s)	272, 1917, 2064, 2244, 2356, 3605
AjuI	GAANNNNNNNTTGG	4 cut(s)	2651, 2683, 3204, 3236
AloI	GAACNNNNNNTCC	4 cut(s)	2943, 2975, 3342, 3374
Alw21I	GWGCWC	2 cut(s)	1762, 2715
Alw44I	GTGCAC	1 cut(s)	1758
AlwI	GGATC	3 cut(s)	182, 251, 917
AlwNI	CAGNNNCTG	2 cut(s)	1361, 3242
Ama87I	CYCGRG	3 cut(s)	124, 440, 869
Aor13HI	TCCGGA	1 cut(s)	2140
Aor51HI	AGCGCT	2 cut(s)	413, 2153
AoxI	GGCC	9 cut(s)	220, 270, 363, 727, 973, 1508, 2888, 3052, 3208
ApaLI	GTGCAC	1 cut(s)	1758
ApeKI	GCWGC	8 cut(s)	779, 1704, 1901, 1904, 2480, 3593, 3596, 3620
ApoI	RAATTY	7 cut(s)	676, 1142, 1375, 1616, 1835, 2114, 3611
ArsI	GACNNNNNNTTYG	4 cut(s)	273, 305, 343, 375
AseI	ATTAAT	1 cut(s)	2279
Asp700I	GAANNNNTTC	3 cut(s)	2971, 3342, 3385
AspLEI	GCGC	7 cut(s)	26, 51, 414, 420, 448, 636, 2154
AspS9I	GGNCC	5 cut(s)	270, 425, 1509, 2876, 3053
AsuC2I	CCSGG	4 cut(s)	125, 126, 1741, 3519
AsuHPI	GGTGA	2 cut(s)	830, 1121
AsuII	TTCGAA	2 cut(s)	2119, 3615
AsuNHI	GCTAGC	2 cut(s)	2174, 3406
AvaI	CYCGRG	3 cut(s)	124, 440, 869
AvaII	GGWCC	2 cut(s)	425, 2876
BaeGI	GKGCMC	1 cut(s)	1762
BaeI	ACNNNNGTAYC	4 cut(s)	2021, 2054, 2979, 3012
BalI	TGGCCA	2 cut(s)	365, 975
BanII	GRGCYC	1 cut(s)	2715
BauI	CACGAG	1 cut(s)	3737
BbsI	GAAGAC	5 cut(s)	474, 561, 701, 2161, 2360
Bbv12I	GWGCWC	2 cut(s)	1762, 2715
BbvI	GCAGC	8 cut(s)	766, 1691, 1891, 1913, 2467, 3580, 3583, 3607
BccI	CCATC	8 cut(s)	97, 332, 1027, 1216, 1742, 2399, 3175, 3314
BceAI	ACGGC	3 cut(s)	37, 235, 2007
BcgI	CGANNNNNNTGC	2 cut(s)	1886, 1920
BciT130I	CCWGG	6 cut(s)	274, 1919, 2066, 2246, 2358, 3607
BcnI	CCSGG	4 cut(s)	125, 126, 1741, 3519
BcuI	ACTAGT	4 cut(s)	532, 1519, 1588, 3731
BfmI	CTRYAG	2 cut(s)	1660, 3135
BfoI	RGCGCY	3 cut(s)	52, 415, 2155
BfrI	CTTAAG	1 cut(s)	2012
BmcAI	AGTACT	1 cut(s)	1563
Bme18I	GGWCC	2 cut(s)	425, 2876
BmeRI	GACNNNNNGTC	1 cut(s)	2810
BmeT110I	CYCGRG	3 cut(s)	124, 440, 869
BmgBI	CACGTC	2 cut(s)	8, 3302
BmgT120I	GGNCC	5 cut(s)	270, 425, 1509, 2876, 3053
BmiI	GGNNCC	6 cut(s)	130, 643, 1605, 1738, 2877, 2878
BmrI	ACTGGG	2 cut(s)	265, 3109
BmtI	GCTAGC	2 cut(s)	2178, 3410
BmuI	ACTGGG	2 cut(s)	265, 3109
BpiI	GAAGAC	5 cut(s)	474, 561, 701, 2161, 2360
BplI	GAGNNNNNCTC	4 cut(s)	1232, 1264, 2617, 2649
BpmI	CTGGAG	3 cut(s)	596, 1940, 2228
Bpu10I	CCTNAGC	1 cut(s)	174
Bpu14I	TTCGAA	2 cut(s)	2119, 3615
BpuEI	CTTGAG	2 cut(s)	716, 2062
BpuMI	CCSGG	4 cut(s)	125, 126, 1741, 3519
Bsa29I	ATCGAT	1 cut(s)	2561
BsaI	GGTCTC	3 cut(s)	386, 2023, 2818
BsaJI	CCNNGG	7 cut(s)	124, 421, 441, 868, 1512, 1740, 3606
BsaWI	WCCGGW	2 cut(s)	644, 2140
Bse3DI	GCAATG	4 cut(s)	1817, 2133, 3330, 3549
BseAI	TCCGGA	1 cut(s)	2140
BseBI	CCWGG	6 cut(s)	274, 1919, 2066, 2246, 2358, 3607
BseCI	ATCGAT	1 cut(s)	2561
BseDI	CCNNGG	7 cut(s)	124, 421, 441, 868, 1512, 1740, 3606
BseMI	GCAATG	4 cut(s)	1817, 2133, 3330, 3549
BseRI	GAGGAG	8 cut(s)	467, 470, 554, 557, 560, 563, 590, 593
BseSI	GKGCMC	1 cut(s)	1762
BseXI	GCAGC	8 cut(s)	766, 1691, 1891, 1913, 2467, 3580, 3583, 3607
BseYI	CCCAGC	1 cut(s)	119
BsgI	GTGCAG	3 cut(s)	1302, 1495, 1890
Bsh1236I	CGCG	3 cut(s)	423, 636, 2268
BshFI	GGCC	9 cut(s)	222, 272, 365, 729, 975, 1510, 2890, 3054, 3210
BshVI	ATCGAT	1 cut(s)	2561
BsiHKAI	GWGCWC	2 cut(s)	1762, 2715
BsiHKCI	CYCGRG	3 cut(s)	124, 440, 869
BsiSI	CCGG	6 cut(s)	125, 495, 645, 1740, 2141, 3519
BslFI	GGGAC	6 cut(s)	621, 1972, 2889, 2964, 2981, 3319
BsmBI	CGTCTC	1 cut(s)	1544
BsmFI	GGGAC	6 cut(s)	621, 1972, 2889, 2964, 2981, 3319
BsmI	GAATGC	1 cut(s)	2203
BsnI	GGCC	9 cut(s)	222, 272, 365, 729, 975, 1510, 2890, 3054, 3210
Bso31I	GGTCTC	3 cut(s)	386, 2023, 2818
BsoBI	CYCGRG	3 cut(s)	124, 440, 869
Bsp119I	TTCGAA	2 cut(s)	2119, 3615
Bsp1286I	GDGCHC	2 cut(s)	1762, 2715
Bsp13I	TCCGGA	1 cut(s)	2140
Bsp143I	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
BspANI	GGCC	9 cut(s)	222, 272, 365, 729, 975, 1510, 2890, 3054, 3210
BspDI	ATCGAT	1 cut(s)	2561
BspEI	TCCGGA	1 cut(s)	2140
BspFNI	CGCG	3 cut(s)	423, 636, 2268
BspHI	TCATGA	2 cut(s)	2452, 2898
BspLI	GGNNCC	6 cut(s)	130, 643, 1605, 1738, 2877, 2878
BspMAI	CTGCAG	1 cut(s)	3139
BspOI	GCTAGC	2 cut(s)	2178, 3410
BspPI	GGATC	3 cut(s)	182, 251, 917
BspT104I	TTCGAA	2 cut(s)	2119, 3615
BspTI	CTTAAG	1 cut(s)	2012
BspTNI	GGTCTC	3 cut(s)	386, 2023, 2818
BsrBI	CCGCTC	1 cut(s)	666
BsrDI	GCAATG	4 cut(s)	1817, 2133, 3330, 3549
BssECI	CCNNGG	7 cut(s)	124, 421, 441, 868, 1512, 1740, 3606
BssMI	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
BssSI	CACGAG	1 cut(s)	3737
Bst2BI	CACGAG	1 cut(s)	3737
Bst2UI	CCWGG	6 cut(s)	274, 1919, 2066, 2246, 2358, 3607
Bst4CI	ACNGT	7 cut(s)	397, 473, 1455, 1639, 1985, 2808, 3374
Bst6I	CTCTTC	3 cut(s)	342, 1957, 1997
BstAFI	CTTAAG	1 cut(s)	2012
BstBI	TTCGAA	2 cut(s)	2119, 3615
BstDSI	CCRYGG	2 cut(s)	421, 1512
BstEII	GGTNACC	2 cut(s)	1178, 3706
BstENI	CCTNNNNNAGG	3 cut(s)	829, 2361, 2454
BstFNI	CGCG	3 cut(s)	423, 636, 2268
BstH2I	RGCGCY	3 cut(s)	52, 415, 2155
BstHHI	GCGC	7 cut(s)	26, 51, 414, 420, 448, 636, 2154
BstKTI	GATC	9 cut(s)	190, 246, 268, 925, 1093, 2505, 2565, 3166, 3196
BstMBI	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
BstMWI	GCNNNNNNNGC	5 cut(s)	420, 1704, 1766, 3093, 3560
BstNI	CCWGG	6 cut(s)	274, 1919, 2066, 2246, 2358, 3607
BstNSI	RCATGY	4 cut(s)	716, 1157, 2487, 3459
BstPI	GGTNACC	2 cut(s)	1178, 3706
BstSFI	CTRYAG	2 cut(s)	1660, 3135
BstSLI	GKGCMC	1 cut(s)	1762
BstUI	CGCG	3 cut(s)	423, 636, 2268
BstV1I	GCAGC	8 cut(s)	766, 1691, 1891, 1913, 2467, 3580, 3583, 3607
BstV2I	GAAGAC	5 cut(s)	474, 561, 701, 2161, 2360
BstXI	CCANNNNNNTGG	1 cut(s)	3718
Bsu15I	ATCGAT	1 cut(s)	2561
BsuRI	GGCC	9 cut(s)	222, 272, 365, 729, 975, 1510, 2890, 3054, 3210
BsuTUI	ATCGAT	1 cut(s)	2561
BtgI	CCRYGG	2 cut(s)	421, 1512
BtgZI	GCGATG	1 cut(s)	513
BtrI	CACGTC	2 cut(s)	8, 3302
BtsI	GCAGTG	2 cut(s)	1362, 1483
BtsIMutI	CAGTG	7 cut(s)	478, 1362, 1483, 1981, 3048, 3284, 3370
CaiI	CAGNNNCTG	2 cut(s)	1361, 3242
CciI	TCATGA	2 cut(s)	2452, 2898
CfoI	GCGC	7 cut(s)	26, 51, 414, 420, 448, 636, 2154
Cfr13I	GGNCC	5 cut(s)	270, 425, 1509, 2876, 3053
Cfr42I	CCGCGG	1 cut(s)	424
Cfr9I	CCCGGG	1 cut(s)	124
ClaI	ATCGAT	1 cut(s)	2561
CseI	GACGC	2 cut(s)	2486, 2597
CsiI	ACCWGGT	1 cut(s)	2356
CspCI	CAANNNNNGTGG	8 cut(s)	688, 723, 1137, 1172, 1225, 1260, 2190, 2225
DpnI	GATC	9 cut(s)	189, 245, 267, 924, 1092, 2504, 2564, 3165, 3195
DpnII	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
DraI	TTTAAA	1 cut(s)	2391
DraIII	CACNNNGTG	1 cut(s)	1987
DrdI	GACNNNNNNGTC	2 cut(s)	2883, 3232
DriI	GACNNNNNGTC	1 cut(s)	2810
DseDI	GACNNNNNNGTC	2 cut(s)	2883, 3232
EaeI	YGGCCR	3 cut(s)	220, 363, 973
Eam1104I	CTCTTC	3 cut(s)	342, 1957, 1997
Eam1105I	GACNNNNNGTC	1 cut(s)	2810
EarI	CTCTTC	3 cut(s)	342, 1957, 1997
EciI	GGCGGA	1 cut(s)	684
Ecl136II	GAGCTC	1 cut(s)	2713
Eco24I	GRGCYC	1 cut(s)	2715
Eco31I	GGTCTC	3 cut(s)	386, 2023, 2818
Eco47I	GGWCC	2 cut(s)	425, 2876
Eco47III	AGCGCT	2 cut(s)	413, 2153
Eco53kI	GAGCTC	1 cut(s)	2713
Eco57I	CTGAAG	5 cut(s)	311, 1938, 3300, 3669, 3795
Eco88I	CYCGRG	3 cut(s)	124, 440, 869
Eco91I	GGTNACC	2 cut(s)	1178, 3706
EcoICRI	GAGCTC	1 cut(s)	2713
EcoNI	CCTNNNNNAGG	3 cut(s)	829, 2361, 2454
EcoO109I	RGGNCCY	1 cut(s)	2876
EcoO65I	GGTNACC	2 cut(s)	1178, 3706
EcoRI	GAATTC	2 cut(s)	1616, 3611
EcoRII	CCWGG	6 cut(s)	272, 1917, 2064, 2244, 2356, 3605
EcoT22I	ATGCAT	1 cut(s)	1155
EcoT38I	GRGCYC	1 cut(s)	2715
Esp3I	CGTCTC	1 cut(s)	1544
FalI	AAGNNNNNCTT	6 cut(s)	2393, 2425, 2654, 2686, 2959, 2991
FaqI	GGGAC	6 cut(s)	621, 1972, 2889, 2964, 2981, 3319
FauNDI	CATATG	3 cut(s)	1683, 3450, 3625
FblI	GTMKAC	1 cut(s)	3743
FriOI	GRGCYC	1 cut(s)	2715
GlaI	GCGC	7 cut(s)	25, 50, 413, 419, 447, 635, 2153
GsaI	CCCAGC	1 cut(s)	123
GsuI	CTGGAG	3 cut(s)	596, 1940, 2228
HaeII	RGCGCY	3 cut(s)	52, 415, 2155
HaeIII	GGCC	9 cut(s)	222, 272, 365, 729, 975, 1510, 2890, 3054, 3210
HapII	CCGG	6 cut(s)	125, 495, 645, 1740, 2141, 3519
HgaI	GACGC	2 cut(s)	2486, 2597
HhaI	GCGC	7 cut(s)	26, 51, 414, 420, 448, 636, 2154
Hin6I	GCGC	7 cut(s)	24, 49, 412, 418, 446, 634, 2152
HinP1I	GCGC	7 cut(s)	24, 49, 412, 418, 446, 634, 2152
HincII	GTYRAC	7 cut(s)	2311, 2380, 3075, 3547, 3679, 3729, 3744
HindII	GTYRAC	7 cut(s)	2311, 2380, 3075, 3547, 3679, 3729, 3744
HindIII	AAGCTT	1 cut(s)	3034
HpaI	GTTAAC	1 cut(s)	3679
HpaII	CCGG	6 cut(s)	125, 495, 645, 1740, 2141, 3519
HphI	GGTGA	2 cut(s)	830, 1121
Hpy99I	CGWCG	6 cut(s)	12, 15, 338, 347, 1236, 2480
HpyAV	CCTTC	9 cut(s)	73, 162, 275, 1099, 1168, 2108, 2187, 2450, 3053
HpyCH4III	ACNGT	7 cut(s)	397, 473, 1455, 1639, 1985, 2808, 3374
HpyCH4IV	ACGT	4 cut(s)	7, 1270, 2650, 3301
HpyF10VI	GCNNNNNNNGC	5 cut(s)	420, 1704, 1766, 3093, 3560
HpySE526I	ACGT	4 cut(s)	7, 1270, 2650, 3301
HspAI	GCGC	7 cut(s)	24, 49, 412, 418, 446, 634, 2152
KflI	GGGWCCC	1 cut(s)	2876
Kpn2I	TCCGGA	1 cut(s)	2140
KspAI	GTTAAC	1 cut(s)	3679
KspI	CCGCGG	1 cut(s)	424
Kzo9I	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
Lsp1109I	GCAGC	8 cut(s)	766, 1691, 1891, 1913, 2467, 3580, 3583, 3607
MabI	ACCWGGT	1 cut(s)	2356
MaeII	ACGT	4 cut(s)	7, 1270, 2650, 3301
MalI	GATC	9 cut(s)	189, 245, 267, 924, 1092, 2504, 2564, 3165, 3195
MbiI	CCGCTC	1 cut(s)	666
MboI	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
MfeI	CAATTG	3 cut(s)	1973, 2577, 2853
MhlI	GDGCHC	2 cut(s)	1762, 2715
MlsI	TGGCCA	2 cut(s)	365, 975
MluNI	TGGCCA	2 cut(s)	365, 975
MlyI	GAGTC	9 cut(s)	603, 1943, 2057, 2316, 2516, 2747, 3508, 3749, 3758
MmeI	TCCRAC	1 cut(s)	3445
Mox20I	TGGCCA	2 cut(s)	365, 975
Mph1103I	ATGCAT	1 cut(s)	1155
MroI	TCCGGA	1 cut(s)	2140
MroXI	GAANNNNTTC	3 cut(s)	2971, 3342, 3385
MscI	TGGCCA	2 cut(s)	365, 975
Msp20I	TGGCCA	2 cut(s)	365, 975
MspA1I	CMGCKG	2 cut(s)	423, 1904
MspCI	CTTAAG	1 cut(s)	2012
MspI	CCGG	6 cut(s)	125, 495, 645, 1740, 2141, 3519
MunI	CAATTG	3 cut(s)	1973, 2577, 2853
Mva1269I	GAATGC	1 cut(s)	2203
MvaI	CCWGG	6 cut(s)	274, 1919, 2066, 2246, 2358, 3607
MvnI	CGCG	3 cut(s)	423, 636, 2268
MwoI	GCNNNNNNNGC	5 cut(s)	420, 1704, 1766, 3093, 3560
NciI	CCSGG	4 cut(s)	125, 126, 1741, 3519
NdeI	CATATG	3 cut(s)	1683, 3450, 3625
NdeII	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
NheI	GCTAGC	2 cut(s)	2174, 3406
NlaIV	GGNNCC	6 cut(s)	130, 643, 1605, 1738, 2877, 2878
NmuCI	GTSAC	7 cut(s)	3, 1357, 1987, 2572, 2818, 3493, 3734
NsiI	ATGCAT	1 cut(s)	1155
NspI	RCATGY	4 cut(s)	716, 1157, 2487, 3459
NspV	TTCGAA	2 cut(s)	2119, 3615
PaeI	GCATGC	1 cut(s)	3459
PagI	TCATGA	2 cut(s)	2452, 2898
PciI	ACATGT	1 cut(s)	712
PcsI	WCGNNNNNNNCGW	2 cut(s)	339, 342
PctI	GAATGC	1 cut(s)	2203
PdmI	GAANNNNTTC	3 cut(s)	2971, 3342, 3385
PflMI	CCANNNNNTGG	2 cut(s)	229, 1856
PfoI	TCCNGGA	1 cut(s)	2244
PleI	GAGTC	9 cut(s)	603, 1943, 2056, 2315, 2515, 2746, 3508, 3748, 3758
PpsI	GAGTC	9 cut(s)	603, 1943, 2056, 2315, 2515, 2746, 3508, 3748, 3758
PpuMI	RGGWCCY	1 cut(s)	2876
PscI	ACATGT	1 cut(s)	712
PshBI	ATTAAT	1 cut(s)	2279
Psp124BI	GAGCTC	1 cut(s)	2715
Psp5II	RGGWCCY	1 cut(s)	2876
Psp6I	CCWGG	6 cut(s)	272, 1917, 2064, 2244, 2356, 3605
PspEI	GGTNACC	2 cut(s)	1178, 3706
PspFI	CCCAGC	1 cut(s)	119
PspGI	CCWGG	6 cut(s)	272, 1917, 2064, 2244, 2356, 3605
PspN4I	GGNNCC	6 cut(s)	130, 643, 1605, 1738, 2877, 2878
PspPI	GGNCC	5 cut(s)	270, 425, 1509, 2876, 3053
PspPPI	RGGWCCY	1 cut(s)	2876
PstI	CTGCAG	1 cut(s)	3139
PstNI	CAGNNNCTG	2 cut(s)	1361, 3242
PvuII	CAGCTG	1 cut(s)	1904
SacI	GAGCTC	1 cut(s)	2715
SacII	CCGCGG	1 cut(s)	424
SalI	GTCGAC	1 cut(s)	3742
Sau3AI	GATC	9 cut(s)	187, 243, 265, 922, 1090, 2502, 2562, 3163, 3193
Sau96I	GGNCC	5 cut(s)	270, 425, 1509, 2876, 3053
ScaI	AGTACT	1 cut(s)	1563
SchI	GAGTC	9 cut(s)	603, 1943, 2057, 2316, 2516, 2747, 3508, 3749, 3758
SduI	GDGCHC	2 cut(s)	1762, 2715
SexAI	ACCWGGT	1 cut(s)	2356
SfcI	CTRYAG	2 cut(s)	1660, 3135
Sfr303I	CCGCGG	1 cut(s)	424
SfuI	TTCGAA	2 cut(s)	2119, 3615
SgrBI	CCGCGG	1 cut(s)	424
SinI	GGWCC	2 cut(s)	425, 2876
SmaI	CCCGGG	1 cut(s)	126
SmlI	CTYRAG	4 cut(s)	731, 2012, 2077, 3800
SmoI	CTYRAG	4 cut(s)	731, 2012, 2077, 3800
SpeI	ACTAGT	4 cut(s)	532, 1519, 1588, 3731
SphI	GCATGC	1 cut(s)	3459
SspI	AATATT	2 cut(s)	814, 2692
SstI	GAGCTC	1 cut(s)	2715
TaaI	ACNGT	7 cut(s)	397, 473, 1455, 1639, 1985, 2808, 3374
TaiI	ACGT	4 cut(s)	10, 1273, 2653, 3304
TatI	WGTACW	3 cut(s)	252, 1561, 3215
TauI	GCSGC	6 cut(s)	423, 639, 642, 669, 2231, 2288
TscAI	CASTG	7 cut(s)	478, 1362, 1483, 1988, 3055, 3291, 3377
TseFI	GTSAC	7 cut(s)	3, 1357, 1987, 2572, 2818, 3493, 3734
TseI	GCWGC	8 cut(s)	779, 1704, 1901, 1904, 2480, 3593, 3596, 3620
Tsp45I	GTSAC	7 cut(s)	3, 1357, 1987, 2572, 2818, 3493, 3734
TspDTI	ATGAA	9 cut(s)	1062, 1118, 2330, 2469, 2643, 2658, 2895, 2934, 3306
TspGWI	ACGGA	4 cut(s)	670, 851, 1568, 3242
TspMI	CCCGGG	1 cut(s)	124
TspRI	CASTG	7 cut(s)	478, 1362, 1483, 1988, 3055, 3291, 3377
Van91I	CCANNNNNTGG	2 cut(s)	229, 1856
Vha464I	CTTAAG	1 cut(s)	2012
VneI	GTGCAC	1 cut(s)	1758
VpaK11BI	GGWCC	2 cut(s)	425, 2876
VspI	ATTAAT	1 cut(s)	2279
XagI	CCTNNNNNAGG	3 cut(s)	829, 2361, 2454
XapI	RAATTY	7 cut(s)	676, 1142, 1375, 1616, 1835, 2114, 3611
XbaI	TCTAGA	1 cut(s)	1807
XceI	RCATGY	4 cut(s)	716, 1157, 2487, 3459
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3467
XmaI	CCCGGG	1 cut(s)	124
XmiI	GTMKAC	1 cut(s)	3743
XmnI	GAANNNNTTC	3 cut(s)	2971, 3342, 3385
ZrmI	AGTACT	1 cut(s)	1563
Zsp2I	ATGCAT	1 cut(s)	1155

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.