AT1G01750

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008931
Physical Location & Seq
Forward (+)
275188 .. 276310
1123 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01750.1

Sequence Viewer

Length: 423 bp
ATGGCTAATTCAGCGTCTGGGATGCACGTTAGCGATGAGTGCAAGCTCAAGTTCTTGGAGTTAAAAGCAAAGAGGAACTATAGGTTCATTGTGTTCAAAATTGATGAGAAGGCTCAGCAAGTTATGATAGACAAGCTTGGGAATCCAGAAGAAACTTACGAAGATTTCACCAGATCTATTCCCGAGGATGAGTGCCGATATGCTGTCTATGACTATGATTTCACCACCCCTGAGAACTGCCAGAAGAGCAAAATCTTTTTCATCGCATGGTCACCTGATACATCAAGAGTGAGGAGTAAGATGTTGTATGCAAGCTCAAAGGACAGGTTCAAGAGGGAATTGGATGGGATTCAAGTTGAATTGCAAGCAACAGATCCTAGCGAGATGAGCCTCGACATCATCAAGGGACGAGTCAATCTCTGA

Protein Analysis

140

Amino Acids

16.34

Weight (kDa)

5.54

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 138 2.6e-43 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 368
AgsI TTSAA 4 cut(s) 97, 331, 353, 359
AluBI AGCT 3 cut(s) 46, 136, 315
AluI AGCT 3 cut(s) 46, 136, 315
AlwI GGATC 1 cut(s) 368
AlwNI CAGNNNCTG 1 cut(s) 17
Ama87I CYCGRG 1 cut(s) 182
AsuHPI GGTGA 3 cut(s) 160, 214, 264
AvaI CYCGRG 1 cut(s) 182
BccI CCATC 1 cut(s) 338
BfaI CTAG 1 cut(s) 378
BfmI CTRYAG 1 cut(s) 79
BglII AGATCT 1 cut(s) 173
BlpI GCTNAGC 1 cut(s) 114
BmeT110I CYCGRG 1 cut(s) 182
BmsI GCATC 1 cut(s) 12
BplI GAGNNNNNCTC 1 cut(s) 402
Bpu1102I GCTNAGC 1 cut(s) 114
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 183
BseDI CCNNGG 1 cut(s) 183
BseGI GGATG 3 cut(s) 27, 193, 349
BseMII CTCAG 2 cut(s) 128, 222
BseRI GAGGAG 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 182
BsoBI CYCGRG 1 cut(s) 182
Bsp143I GATC 2 cut(s) 173, 373
Bsp1720I GCTNAGC 1 cut(s) 114
BspCNI CTCAG 2 cut(s) 127, 223
BspPI GGATC 1 cut(s) 368
BspQI GCTCTTC 1 cut(s) 239
BssECI CCNNGG 1 cut(s) 183
BssMI GATC 2 cut(s) 173, 373
Bst6I CTCTTC 1 cut(s) 239
BstC8I GCNNGC 3 cut(s) 44, 313, 366
BstDEI CTNAG 2 cut(s) 114, 231
BstEII GGTNACC 1 cut(s) 270
BstF5I GGATG 3 cut(s) 27, 193, 349
BstKTI GATC 2 cut(s) 176, 376
BstMBI GATC 2 cut(s) 173, 373
BstMWI GCNNNNNNNGC 4 cut(s) 11, 39, 246, 387
BstPI GGTNACC 1 cut(s) 270
BstSFI CTRYAG 1 cut(s) 79
BstX2I RGATCY 2 cut(s) 173, 373
BstYI RGATCY 2 cut(s) 173, 373
BtgZI GCGATG 2 cut(s) 48, 247
BtsCI GGATG 3 cut(s) 27, 193, 349
Cac8I GCNNGC 3 cut(s) 44, 313, 366
CaiI CAGNNNCTG 1 cut(s) 17
CseI GACGC 1 cut(s) 3
CviAII CATG 1 cut(s) 267
CviJI RGCY 6 cut(s) 5, 46, 113, 136, 315, 390
CviKI_1 RGCY 6 cut(s) 5, 46, 113, 136, 315, 390
DdeI CTNAG 2 cut(s) 114, 231
DpnI GATC 2 cut(s) 175, 375
DpnII GATC 2 cut(s) 173, 373
Eam1104I CTCTTC 1 cut(s) 239
EarI CTCTTC 1 cut(s) 239
Eco88I CYCGRG 1 cut(s) 182
Eco91I GGTNACC 1 cut(s) 270
EcoO65I GGTNACC 1 cut(s) 270
FaeI CATG 1 cut(s) 270
FaiI YATR 7 cut(s) 81, 125, 201, 210, 216, 268, 309
FatI CATG 1 cut(s) 266
FokI GGATG 3 cut(s) 34, 200, 356
FspBI CTAG 1 cut(s) 378
HgaI GACGC 1 cut(s) 3
Hin1II CATG 1 cut(s) 270
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 3 cut(s) 142, 349, 411
HphI GGTGA 3 cut(s) 160, 214, 264
Hpy188I TCNGA 1 cut(s) 422
Hpy188III TCNNGA 4 cut(s) 146, 182, 285, 331
HpyAV CCTTC 1 cut(s) 103
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 4 cut(s) 25, 42, 311, 364
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 39, 246, 387
HpyF3I CTNAG 2 cut(s) 114, 231
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 270
Kzo9I GATC 2 cut(s) 173, 373
LguI GCTCTTC 1 cut(s) 239
LpnPI CCDG 7 cut(s) 3, 159, 184, 243, 254, 288, 310
LweI GCATC 1 cut(s) 12
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 27
MaeIII GTNAC 1 cut(s) 270
MalI GATC 2 cut(s) 175, 375
MboI GATC 2 cut(s) 173, 373
MboII GAAGA 3 cut(s) 161, 173, 256
MflI RGATCY 2 cut(s) 173, 373
MluCI AATT 4 cut(s) 7, 99, 338, 359
MlyI GAGTC 1 cut(s) 420
MnlI CCTC 5 cut(s) 66, 178, 285, 327, 401
MseI TTAA 1 cut(s) 62
MwoI GCNNNNNNNGC 4 cut(s) 11, 39, 246, 387
NdeII GATC 2 cut(s) 173, 373
NlaIII CATG 1 cut(s) 270
NmuCI GTSAC 1 cut(s) 270
PciSI GCTCTTC 1 cut(s) 239
PfeI GAWTC 2 cut(s) 142, 349
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
PspEI GGTNACC 1 cut(s) 270
PstNI CAGNNNCTG 1 cut(s) 17
PsuI RGATCY 2 cut(s) 173, 373
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 1 cut(s) 62
Sau3AI GATC 2 cut(s) 173, 373
SchI GAGTC 1 cut(s) 420
SetI ASST 7 cut(s) 30, 48, 86, 138, 277, 317, 329
SfaNI GCATC 1 cut(s) 12
SfcI CTRYAG 1 cut(s) 79
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 4 cut(s) 7, 99, 338, 359
SspMI CTAG 1 cut(s) 378
TaiI ACGT 1 cut(s) 30
TaqI TCGA 1 cut(s) 393
TasI AATT 4 cut(s) 7, 99, 338, 359
TfiI GAWTC 2 cut(s) 142, 349
Tru1I TTAA 1 cut(s) 62
Tru9I TTAA 1 cut(s) 62
TseFI GTSAC 1 cut(s) 270
Tsp45I GTSAC 1 cut(s) 270
TspDTI ATGAA 2 cut(s) 76, 250
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.