Rorug01G0241500

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0008931
Physical Location & Seq
Reverse (-)
34639646 .. 34640173
528 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0241500.1

Sequence Viewer

Length: 528 bp
ATGTCGGCGACGAGCGCTGGAACCTCAAGAGCGGCTGCATGTCCGTGGGCCTTCTTGTGTCCATTGGAGGAGGGTTTGCCTCATCCTCAACCTATTCCAAAGGCCAAAACATTCGCCTCTGTGCTCTCTAACTCAGTGGAGTCTTCCGTCTCTCTTAGCGAATTGCTTGCTCCAGTCATACGCGGAGACAAAACTTACGTCAAAATCAATGAAGCTCTATACCAGGATCAGTTGAAAATTTTCAAAACCAATCTTATTGGTAGGCTGCTACTGCGAAAGGGTTCTGTCCCAATGAAAACATCCATCCTCAAGAGCTTGCTTGCCGATCTATGGAAGCCATCAGCGCCTTGGCGCTTGGTTTCGCTTGGGAAGGGATACTTTGACATTCACTTTGGCACTGAGGAGGACCTACGACGAGTTTGGGGTGGTGGTACATGCACTCTACCCGACGGCCTCTTCCGACTGGCTCAATGGAAGCCTGACTTTGTGCCTGGGGATGTCCTTCCACAAACACATGCCCAAGTATAG

Protein Analysis

175

Amino Acids

19.19

Weight (kDa)

9.22

Isoelectric Point (pI)

40.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4283 PF14111 83 - 164 2.9e-07 Domain of unknown function (DUF4283)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 1 cut(s) 183
AciI CCGC 2 cut(s) 32, 183
AclWI GGATC 1 cut(s) 234
AcsI RAATTY 1 cut(s) 237
AfaI GTAC 1 cut(s) 433
AfeI AGCGCT 1 cut(s) 16
AfiI CCNNNNNNNGG 1 cut(s) 330
AgsI TTSAA 2 cut(s) 235, 244
AjnI CCWGG 2 cut(s) 222, 490
AluBI AGCT 2 cut(s) 215, 315
AluI AGCT 2 cut(s) 215, 315
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 2 cut(s) 154, 180
AlwI GGATC 1 cut(s) 234
Aor51HI AGCGCT 1 cut(s) 16
AoxI GGCC 3 cut(s) 48, 102, 451
ApeKI GCWGC 2 cut(s) 35, 265
ApoI RAATTY 1 cut(s) 237
ArsI GACNNNNNNTTYG 4 cut(s) 183, 215, 374, 406
Asp700I GAANNNNTTC 2 cut(s) 239, 280
AspLEI GCGC 3 cut(s) 17, 346, 354
AspS9I GGNCC 2 cut(s) 48, 406
AvaII GGWCC 1 cut(s) 406
BbsI GAAGAC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 22, 252
BccI CCATC 2 cut(s) 311, 346
BceAI ACGGC 1 cut(s) 466
BcgI CGANNNNNNTGC 2 cut(s) 149, 183
BciT130I CCWGG 2 cut(s) 224, 492
BciVI GTATCC 1 cut(s) 368
BcoDI GTCTC 2 cut(s) 154, 180
BfoI RGCGCY 3 cut(s) 18, 347, 355
BfuI GTATCC 1 cut(s) 368
BisI GCNGC 3 cut(s) 33, 36, 266
BlsI GCNGC 3 cut(s) 34, 37, 267
Bme1390I CCNGG 2 cut(s) 224, 492
Bme18I GGWCC 1 cut(s) 406
BmgT120I GGNCC 2 cut(s) 48, 406
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 2 cut(s) 224, 492
BpiI GAAGAC 1 cut(s) 135
BpmI CTGGAG 1 cut(s) 156
BpuEI CTTGAG 2 cut(s) 10, 293
BsaJI CCNNGG 3 cut(s) 44, 347, 491
Bsc4I CCNNNNNNNGG 1 cut(s) 330
Bse1I ACTGG 2 cut(s) 173, 468
BseBI CCWGG 2 cut(s) 224, 492
BseDI CCNNGG 3 cut(s) 44, 347, 491
BseGI GGATG 4 cut(s) 82, 299, 303, 502
BseLI CCNNNNNNNGG 1 cut(s) 330
BseMII CTCAG 2 cut(s) 147, 390
BseNI ACTGG 2 cut(s) 173, 468
BseRI GAGGAG 2 cut(s) 83, 416
BseXI GCAGC 2 cut(s) 22, 252
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 3 cut(s) 50, 104, 453
BsiHKAI GWGCWC 1 cut(s) 126
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 1 cut(s) 330
BsmAI GTCTC 2 cut(s) 154, 180
BsmBI CGTCTC 1 cut(s) 154
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 3 cut(s) 50, 104, 453
Bsp1286I GDGCHC 1 cut(s) 126
Bsp143I GATC 2 cut(s) 226, 325
BspACI CCGC 2 cut(s) 32, 183
BspANI GGCC 3 cut(s) 50, 104, 453
BspCNI CTCAG 2 cut(s) 146, 391
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 22
BspPI GGATC 1 cut(s) 234
BsrBI CCGCTC 1 cut(s) 32
BsrI ACTGG 2 cut(s) 173, 468
BssECI CCNNGG 3 cut(s) 44, 347, 491
BssMI GATC 2 cut(s) 226, 325
BssT1I CCWWGG 1 cut(s) 347
Bst2UI CCWGG 2 cut(s) 224, 492
Bst6I CTCTTC 1 cut(s) 461
BstC8I GCNNGC 3 cut(s) 168, 317, 321
BstDEI CTNAG 3 cut(s) 133, 155, 399
BstDSI CCRYGG 1 cut(s) 44
BstF5I GGATG 4 cut(s) 82, 299, 303, 502
BstFNI CGCG 1 cut(s) 183
BstH2I RGCGCY 3 cut(s) 18, 347, 355
BstHHI GCGC 3 cut(s) 17, 346, 354
BstKTI GATC 2 cut(s) 229, 328
BstMAI GTCTC 2 cut(s) 154, 180
BstMBI GATC 2 cut(s) 226, 325
BstMWI GCNNNNNNNGC 3 cut(s) 14, 271, 343
BstNI CCWGG 2 cut(s) 224, 492
BstNSI RCATGY 3 cut(s) 42, 438, 518
BstSCI CCNGG 2 cut(s) 222, 490
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 2 cut(s) 22, 252
BstV2I GAAGAC 1 cut(s) 135
BsuI GTATCC 1 cut(s) 368
BsuRI GGCC 3 cut(s) 50, 104, 453
BtgI CCRYGG 1 cut(s) 44
BtsCI GGATG 4 cut(s) 82, 299, 303, 502
BtsIMutI CAGTG 2 cut(s) 141, 396
Cac8I GCNNGC 3 cut(s) 168, 317, 321
CfoI GCGC 3 cut(s) 17, 346, 354
Cfr13I GGNCC 2 cut(s) 48, 406
Csp6I GTAC 1 cut(s) 432
CviAII CATG 3 cut(s) 39, 435, 515
CviQI GTAC 1 cut(s) 432
DdeI CTNAG 3 cut(s) 133, 155, 399
DpnI GATC 2 cut(s) 228, 327
DpnII GATC 2 cut(s) 226, 325
Eam1104I CTCTTC 1 cut(s) 461
EarI CTCTTC 1 cut(s) 461
Eco130I CCWWGG 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 406
Eco47III AGCGCT 1 cut(s) 16
EcoO109I RGGNCCY 1 cut(s) 406
EcoRII CCWGG 2 cut(s) 222, 490
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
Esp3I CGTCTC 1 cut(s) 154
FaeI CATG 3 cut(s) 42, 438, 518
FaiI YATR 7 cut(s) 40, 179, 220, 331, 436, 516, 526
FalI AAGNNNNNCTT 4 cut(s) 362, 394, 467, 499
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 38, 434, 514
Fnu4HI GCNGC 3 cut(s) 33, 36, 266
FokI GGATG 4 cut(s) 69, 286, 290, 509
Fsp4HI GCNGC 3 cut(s) 33, 36, 266
GlaI GCGC 3 cut(s) 16, 345, 353
GluI GCNGC 3 cut(s) 33, 36, 266
GsuI CTGGAG 1 cut(s) 156
HaeII RGCGCY 3 cut(s) 18, 347, 355
HaeIII GGCC 3 cut(s) 50, 104, 453
HhaI GCGC 3 cut(s) 17, 346, 354
Hin1II CATG 3 cut(s) 42, 438, 518
Hin6I GCGC 3 cut(s) 15, 344, 352
HinP1I GCGC 3 cut(s) 15, 344, 352
HinfI GANTC 1 cut(s) 140
Hpy188I TCNGA 1 cut(s) 461
Hpy188III TCNNGA 2 cut(s) 27, 310
Hpy99I CGWCG 3 cut(s) 13, 417, 452
HpyAV CCTTC 3 cut(s) 61, 364, 512
HpyCH4IV ACGT 1 cut(s) 198
HpyCH4V TGCA 2 cut(s) 38, 438
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 271, 343
HpyF3I CTNAG 3 cut(s) 133, 155, 399
HpySE526I ACGT 1 cut(s) 198
Hsp92II CATG 3 cut(s) 42, 438, 518
HspAI GCGC 3 cut(s) 15, 344, 352
Kzo9I GATC 2 cut(s) 226, 325
LmnI GCTCC 1 cut(s) 175
LpnPI CCDG 8 cut(s) 3, 186, 209, 236, 449, 477, 492, 504
Lsp1109I GCAGC 2 cut(s) 22, 252
MaeII ACGT 1 cut(s) 198
MalI GATC 2 cut(s) 228, 327
MbiI CCGCTC 1 cut(s) 32
MboI GATC 2 cut(s) 226, 325
MboII GAAGA 2 cut(s) 135, 448
MhlI GDGCHC 1 cut(s) 126
MluCI AATT 2 cut(s) 161, 237
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 1 cut(s) 484
MroXI GAANNNNTTC 2 cut(s) 239, 280
MslI CAYNNNNRTG 1 cut(s) 43
MspR9I CCNGG 2 cut(s) 224, 492
MvaI CCWGG 2 cut(s) 224, 492
MvnI CGCG 1 cut(s) 183
MwoI GCNNNNNNNGC 3 cut(s) 14, 271, 343
NdeII GATC 2 cut(s) 226, 325
NlaIII CATG 3 cut(s) 42, 438, 518
NlaIV GGNNCC 1 cut(s) 22
NspI RCATGY 3 cut(s) 42, 438, 518
PdmI GAANNNNTTC 2 cut(s) 239, 280
PkrI GCNGC 3 cut(s) 34, 37, 267
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 406
Psp5II RGGWCCY 1 cut(s) 406
Psp6I CCWGG 2 cut(s) 222, 490
PspGI CCWGG 2 cut(s) 222, 490
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 2 cut(s) 48, 406
PspPPI RGGWCCY 1 cut(s) 406
RsaI GTAC 1 cut(s) 433
RsaNI GTAC 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 43
SatI GCNGC 3 cut(s) 33, 36, 266
Sau3AI GATC 2 cut(s) 226, 325
Sau96I GGNCC 2 cut(s) 48, 406
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 2 cut(s) 224, 492
SduI GDGCHC 1 cut(s) 126
SetI ASST 6 cut(s) 26, 94, 201, 217, 317, 411
SinI GGWCC 1 cut(s) 406
SmiMI CAYNNNNRTG 1 cut(s) 43
SmlI CTYRAG 2 cut(s) 25, 308
SmoI CTYRAG 2 cut(s) 25, 308
Sse9I AATT 2 cut(s) 161, 237
SsiI CCGC 2 cut(s) 32, 183
StyD4I CCNGG 2 cut(s) 222, 490
StyI CCWWGG 1 cut(s) 347
TaiI ACGT 1 cut(s) 201
TasI AATT 2 cut(s) 161, 237
TauI GCSGC 1 cut(s) 35
TscAI CASTG 2 cut(s) 141, 403
TseI GCWGC 2 cut(s) 35, 265
TspDTI ATGAA 2 cut(s) 225, 308
TspGWI ACGGA 2 cut(s) 33, 136
TspRI CASTG 2 cut(s) 141, 403
VpaK11BI GGWCC 1 cut(s) 406
XapI RAATTY 1 cut(s) 237
XceI RCATGY 3 cut(s) 42, 438, 518
XcmI CCANNNNNNNNNTGG 1 cut(s) 345
XmnI GAANNNNTTC 2 cut(s) 239, 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.