pycom07g13800

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008931
Physical Location & Seq
Forward (+)
15684623 .. 15685459
837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g13800.1

Sequence Viewer

Length: 399 bp
ATGGCTGTGCACGATGATTGTAAGCTCAAATTCTTGGAACTAAAAGCGAAGAGAAATTTCCGATTTATTGTGTTCAAGATCGAGGAGAAGATACAACAGGTGATTGTAGAGAAGCTTGGAGGTCCTGATGAAAGCTACGATGATTTCAGTGCCTCCTTGCCTGCCGATGAGTGCCGCTATGCCGTTTATGATTTTAATTTTACAACCAATGAGAACTGCCAGAAAAGCAAAATATTCTTCATTGCATGGGCACCTGATACATCAAGGGTCAGAAGTAAGATGCTTTATGCAAGCTCCAAGGACAGATTCAAGAGGGAGTTGGAAGGCATTCAAGTGGAGTTGCAGGCAACAGATCCTAGTGAGATGAGCTTGGATATCATAAAAGGCCGGGCACTCTAA

Protein Analysis

133

Amino Acids

15.31

Weight (kDa)

5.9

Isoelectric Point (pI)

36.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 2 - 130 8.4e-42 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 250
AciI CCGC 1 cut(s) 175
AclWI GGATC 1 cut(s) 347
AcsI RAATTY 2 cut(s) 29, 55
AgsI TTSAA 3 cut(s) 76, 310, 332
AjuI GAANNNNNNNTTGG 2 cut(s) 290, 322
AluBI AGCT 5 cut(s) 25, 115, 135, 294, 369
AluI AGCT 5 cut(s) 25, 115, 135, 294, 369
Alw21I GWGCWC 1 cut(s) 12
Alw44I GTGCAC 1 cut(s) 8
AlwI GGATC 1 cut(s) 347
AoxI GGCC 1 cut(s) 385
ApaLI GTGCAC 1 cut(s) 8
ApoI RAATTY 2 cut(s) 29, 55
Asp700I GAANNNNTTC 1 cut(s) 327
AspS9I GGNCC 1 cut(s) 122
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 1 cut(s) 112
AvaII GGWCC 1 cut(s) 122
BaeGI GKGCMC 3 cut(s) 12, 253, 394
BanI GGYRCC 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 12
BceAI ACGGC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 389
BfaI CTAG 1 cut(s) 357
BisI GCNGC 1 cut(s) 175
BlsI GCNGC 1 cut(s) 176
Bme1390I CCNGG 1 cut(s) 389
Bme18I GGWCC 1 cut(s) 122
BmgT120I GGNCC 1 cut(s) 122
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 389
BmsI GCATC 1 cut(s) 270
BpuMI CCSGG 1 cut(s) 389
BsaJI CCNNGG 1 cut(s) 297
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 1 cut(s) 297
BseMI GCAATG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 3 cut(s) 12, 253, 394
BshFI GGCC 1 cut(s) 387
BshNI GGYRCC 1 cut(s) 250
BsiHKAI GWGCWC 1 cut(s) 12
BsiSI CCGG 1 cut(s) 388
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 1 cut(s) 387
Bsp1286I GDGCHC 3 cut(s) 12, 253, 394
Bsp143I GATC 2 cut(s) 78, 352
BspACI CCGC 1 cut(s) 175
BspANI GGCC 1 cut(s) 387
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 1 cut(s) 347
BspT107I GGYRCC 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 1 cut(s) 297
BssMI GATC 2 cut(s) 78, 352
BssT1I CCWWGG 1 cut(s) 297
Bst6I CTCTTC 1 cut(s) 44
BstC8I GCNNGC 3 cut(s) 162, 292, 345
BstKTI GATC 2 cut(s) 81, 355
BstMBI GATC 2 cut(s) 78, 352
BstMWI GCNNNNNNNGC 1 cut(s) 225
BstSCI CCNGG 1 cut(s) 387
BstSLI GKGCMC 3 cut(s) 12, 253, 394
BstX2I RGATCY 1 cut(s) 352
BstYI RGATCY 1 cut(s) 352
BsuRI GGCC 1 cut(s) 387
BtsIMutI CAGTG 1 cut(s) 154
Cac8I GCNNGC 3 cut(s) 162, 292, 345
Cfr13I GGNCC 1 cut(s) 122
CviAII CATG 1 cut(s) 246
CviJI RGCY 7 cut(s) 5, 25, 115, 135, 294, 369, 387
CviKI_1 RGCY 7 cut(s) 5, 25, 115, 135, 294, 369, 387
DpnI GATC 2 cut(s) 80, 354
DpnII GATC 2 cut(s) 78, 352
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco130I CCWWGG 1 cut(s) 297
Eco32I GATATC 1 cut(s) 376
Eco47I GGWCC 1 cut(s) 122
EcoO109I RGGNCCY 1 cut(s) 122
EcoRV GATATC 1 cut(s) 376
EcoT14I CCWWGG 1 cut(s) 297
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 1 cut(s) 249
FaiI YATR 5 cut(s) 180, 189, 247, 288, 380
FatI CATG 1 cut(s) 245
Fnu4HI GCNGC 1 cut(s) 175
Fsp4HI GCNGC 1 cut(s) 175
FspBI CTAG 1 cut(s) 357
GluI GCNGC 1 cut(s) 175
HaeIII GGCC 1 cut(s) 387
HapII CCGG 1 cut(s) 388
Hin1II CATG 1 cut(s) 249
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 1 cut(s) 306
HpaII CCGG 1 cut(s) 388
HphI GGTGA 1 cut(s) 112
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 2 cut(s) 62, 272
Hpy188III TCNNGA 3 cut(s) 76, 125, 310
Hpy8I GTNNAC 1 cut(s) 10
HpyAV CCTTC 1 cut(s) 317
HpyCH4V TGCA 4 cut(s) 10, 245, 290, 343
HpyF10VI GCNNNNNNNGC 1 cut(s) 225
Hsp92II CATG 1 cut(s) 249
Kzo9I GATC 2 cut(s) 78, 352
LmnI GCTCC 1 cut(s) 299
LpnPI CCDG 6 cut(s) 83, 138, 174, 233, 267, 329
LweI GCATC 1 cut(s) 270
MaeI CTAG 1 cut(s) 357
MalI GATC 2 cut(s) 80, 354
MboI GATC 2 cut(s) 78, 352
MboII GAAGA 3 cut(s) 61, 100, 229
MflI RGATCY 1 cut(s) 352
MhlI GDGCHC 3 cut(s) 12, 253, 394
MluCI AATT 3 cut(s) 29, 55, 196
MmeI TCCRAC 1 cut(s) 300
MnlI CCTC 4 cut(s) 76, 113, 163, 306
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 1 cut(s) 195
MslI CAYNNNNRTG 1 cut(s) 332
MspI CCGG 1 cut(s) 388
MspR9I CCNGG 1 cut(s) 389
Mva1269I GAATGC 1 cut(s) 327
MwoI GCNNNNNNNGC 1 cut(s) 225
NciI CCSGG 1 cut(s) 389
NdeII GATC 2 cut(s) 78, 352
NlaIII CATG 1 cut(s) 249
NlaIV GGNNCC 1 cut(s) 252
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 306
PkrI GCNGC 1 cut(s) 176
PpuMI RGGWCCY 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 122
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 122
PspPPI RGGWCCY 1 cut(s) 122
PsuI RGATCY 1 cut(s) 352
RseI CAYNNNNRTG 1 cut(s) 332
SaqAI TTAA 1 cut(s) 195
SatI GCNGC 1 cut(s) 175
Sau3AI GATC 2 cut(s) 78, 352
Sau96I GGNCC 1 cut(s) 122
ScrFI CCNGG 1 cut(s) 389
SduI GDGCHC 3 cut(s) 12, 253, 394
SetI ASST 8 cut(s) 27, 102, 117, 124, 137, 256, 296, 371
SfaNI GCATC 1 cut(s) 270
SinI GGWCC 1 cut(s) 122
SmiMI CAYNNNNRTG 1 cut(s) 332
Sse9I AATT 3 cut(s) 29, 55, 196
SsiI CCGC 1 cut(s) 175
SspI AATATT 1 cut(s) 234
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 1 cut(s) 387
StyI CCWWGG 1 cut(s) 297
TaqI TCGA 1 cut(s) 81
TasI AATT 3 cut(s) 29, 55, 196
TauI GCSGC 1 cut(s) 177
TfiI GAWTC 1 cut(s) 306
Tru1I TTAA 1 cut(s) 195
Tru9I TTAA 1 cut(s) 195
TscAI CASTG 1 cut(s) 154
TspDTI ATGAA 2 cut(s) 144, 229
TspRI CASTG 1 cut(s) 154
VneI GTGCAC 1 cut(s) 8
VpaK11BI GGWCC 1 cut(s) 122
XapI RAATTY 2 cut(s) 29, 55
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.