Rh1BG224100

Belongs to the actin-binding proteins ADF family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0008931
Physical Location & Seq
Reverse (-)
34288685 .. 34290197
1513 bp
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UTR
Exon/CDS
Intron
Rh1BG224100.1

Sequence Viewer

Length: 420 bp
ATGTCGAACTCCGCGTCTGGAATGGCTGTGCATGATGATTGTAAGCTCAAGTTCTTGGAACTGAAAGCGAAGAGGAACCACCGATTCATTGTGTTCAAGATTGAGGAGAAGATACAACAGGTGATTGTAGAGAAGCTTGGAGGTCCTGATGAAAGCTACGAGGACTTCACTAACTCCATACCTGCTGATGAGTGCCGCTATGCTGTCTATGATTTCGATTTCACAACCGATGAGAACTGCCAGAAAAGCAAAATCTTCTTCATTGCATGGTCGCCTGATACGTCGAGGGTGAGAAGTAAGATGCTTTATGCAAGCTCCAAGGACAGATTCAAGAGGGAATTGGATGGCATTCAAGTGGAGTTGCAGGCAACAGATCCTAGTGAGATGAGTTTGGATATCATAAAAGGCCGAGCACTCTAA

Protein Analysis

139

Amino Acids

16.01

Weight (kDa)

5.46

Isoelectric Point (pI)

41.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cofilin_ADF PF00241 7 - 137 6.4e-43 Cofilin/tropomyosin-type actin-binding protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 190
AccII CGCG 1 cut(s) 14
AciI CCGC 2 cut(s) 12, 196
AclWI GGATC 1 cut(s) 368
AgsI TTSAA 3 cut(s) 97, 331, 353
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 4 cut(s) 46, 136, 156, 315
AluI AGCT 4 cut(s) 46, 136, 156, 315
Alw21I GWGCWC 1 cut(s) 415
AlwI GGATC 1 cut(s) 368
AoxI GGCC 1 cut(s) 406
ArsI GACNNNNNNTTYG 1 cut(s) 31
AspS9I GGNCC 1 cut(s) 143
AsuHPI GGTGA 2 cut(s) 133, 301
AvaII GGWCC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 415
BccI CCATC 1 cut(s) 338
BfaI CTAG 1 cut(s) 378
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 1 cut(s) 196
BlsI GCNGC 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 143
BmgT120I GGNCC 1 cut(s) 143
BmiI GGNNCC 1 cut(s) 77
BmsI GCATC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 32
BsaJI CCNNGG 1 cut(s) 318
Bse3DI GCAATG 1 cut(s) 261
BseDI CCNNGG 1 cut(s) 318
BseGI GGATG 1 cut(s) 349
BseMI GCAATG 1 cut(s) 261
BseRI GAGGAG 1 cut(s) 119
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 415
BsmI GAATGC 1 cut(s) 348
BsnI GGCC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 415
Bsp143I GATC 1 cut(s) 373
BspACI CCGC 2 cut(s) 12, 196
BspANI GGCC 1 cut(s) 408
BspFNI CGCG 1 cut(s) 14
BspLI GGNNCC 1 cut(s) 77
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 1 cut(s) 368
BsrDI GCAATG 1 cut(s) 261
BssECI CCNNGG 1 cut(s) 318
BssMI GATC 1 cut(s) 373
BssT1I CCWWGG 1 cut(s) 318
Bst6I CTCTTC 1 cut(s) 65
BstC8I GCNNGC 2 cut(s) 313, 366
BstF5I GGATG 1 cut(s) 349
BstFNI CGCG 1 cut(s) 14
BstKTI GATC 1 cut(s) 376
BstMBI GATC 1 cut(s) 373
BstMWI GCNNNNNNNGC 1 cut(s) 246
BstUI CGCG 1 cut(s) 14
BstX2I RGATCY 1 cut(s) 373
BstYI RGATCY 1 cut(s) 373
BsuRI GGCC 1 cut(s) 408
BtsCI GGATG 1 cut(s) 349
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 313, 366
Cfr13I GGNCC 1 cut(s) 143
CseI GACGC 1 cut(s) 3
CviAII CATG 2 cut(s) 32, 267
CviJI RGCY 6 cut(s) 26, 46, 136, 156, 315, 408
CviKI_1 RGCY 6 cut(s) 26, 46, 136, 156, 315, 408
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
Eam1104I CTCTTC 1 cut(s) 65
EarI CTCTTC 1 cut(s) 65
Eco130I CCWWGG 1 cut(s) 318
Eco32I GATATC 1 cut(s) 397
Eco47I GGWCC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 143
EcoRV GATATC 1 cut(s) 397
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaeI CATG 2 cut(s) 35, 270
FaiI YATR 7 cut(s) 33, 179, 201, 210, 268, 309, 401
FatI CATG 2 cut(s) 31, 266
Fnu4HI GCNGC 1 cut(s) 196
FokI GGATG 1 cut(s) 356
Fsp4HI GCNGC 1 cut(s) 196
FspBI CTAG 1 cut(s) 378
GluI GCNGC 1 cut(s) 196
HaeIII GGCC 1 cut(s) 408
HgaI GACGC 1 cut(s) 3
Hin1II CATG 2 cut(s) 35, 270
HindIII AAGCTT 1 cut(s) 134
HinfI GANTC 2 cut(s) 84, 327
HphI GGTGA 2 cut(s) 133, 301
Hpy188III TCNNGA 4 cut(s) 18, 97, 146, 331
Hpy99I CGWCG 1 cut(s) 286
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 4 cut(s) 31, 266, 311, 364
HpyF10VI GCNNNNNNNGC 1 cut(s) 246
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 2 cut(s) 35, 270
Kzo9I GATC 1 cut(s) 373
LmnI GCTCC 1 cut(s) 320
LpnPI CCDG 7 cut(s) 3, 104, 159, 195, 254, 288, 350
LweI GCATC 1 cut(s) 291
MaeI CTAG 1 cut(s) 378
MaeII ACGT 1 cut(s) 281
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 4 cut(s) 82, 121, 247, 250
MflI RGATCY 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 415
MluCI AATT 1 cut(s) 338
MnlI CCTC 6 cut(s) 66, 97, 134, 154, 279, 327
MslI CAYNNNNRTG 1 cut(s) 353
Mva1269I GAATGC 1 cut(s) 348
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 246
NdeII GATC 1 cut(s) 373
NlaIII CATG 2 cut(s) 35, 270
NlaIV GGNNCC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 278
PctI GAATGC 1 cut(s) 348
PfeI GAWTC 2 cut(s) 84, 327
PkrI GCNGC 1 cut(s) 197
PpuMI RGGWCCY 1 cut(s) 143
Psp5II RGGWCCY 1 cut(s) 143
PspN4I GGNNCC 1 cut(s) 77
PspPI GGNCC 1 cut(s) 143
PspPPI RGGWCCY 1 cut(s) 143
PsuI RGATCY 1 cut(s) 373
RseI CAYNNNNRTG 1 cut(s) 353
SatI GCNGC 1 cut(s) 196
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 1 cut(s) 143
SduI GDGCHC 1 cut(s) 415
SetI ASST 8 cut(s) 48, 123, 138, 145, 158, 184, 284, 317
SfaNI GCATC 1 cut(s) 291
SinI GGWCC 1 cut(s) 143
SmiMI CAYNNNNRTG 1 cut(s) 353
SmlI CTYRAG 1 cut(s) 47
SmoI CTYRAG 1 cut(s) 47
Sse9I AATT 1 cut(s) 338
SsiI CCGC 2 cut(s) 12, 196
SspMI CTAG 1 cut(s) 378
StyI CCWWGG 1 cut(s) 318
TaiI ACGT 1 cut(s) 284
TaqI TCGA 3 cut(s) 5, 216, 284
TasI AATT 1 cut(s) 338
TauI GCSGC 1 cut(s) 198
TfiI GAWTC 2 cut(s) 84, 327
TspDTI ATGAA 3 cut(s) 76, 165, 250
VpaK11BI GGWCC 1 cut(s) 143
XspI CTAG 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.