AT1G01910

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010938
Physical Location & Seq
Reverse (-)
313101 .. 316090
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G01910.5

Sequence Viewer

Length: 1062 bp
ATGGCGGCGGATTTGCCGGAGGCGACAGTACAGAACATTCTGGACCAAGAGTCTCTTAAATGGGTTTTCGTCGGAGGTAAAGGAGGTGTTGGGAAGACGACATGCAGTTCAATTCTCGCAATTTGTCTCGCCAGTGTTCGATCCTCCGTTCTCATCATCTCCACCGATCCTGCTCACAATCTTAGTGATGCCTTTCAGCAACGCTTCACTAAATCTCCCACTTTGGTTCAAGGTTTCTCTAATCTCTTTGCCATGGAGGTAGATCCTACCGTTGAAACTGATGACATGGCTGGTACAGACGGGATGGATGGTTTATTCTCTGATTTGGCAAATGCGATTCCTGGAATCGATGAGGCTATGAGTTTTGCTGAGATGTTGAAGTTGGTGCAAACAATGGATTATGCTACTATTGTGTTTGACACTGCTCCTACTGGACATACTCTCCGCCTGTTACAGTTTCCGGCCACACTAGAAAAGGGACTTTCGAAGTTGATGTCATTGAAGAGTAGATTTGGTGGCTTGATGACTCAGATGAGCCGTATGTTTGGCATGGAGGATGAGTTCGGAGAGGATGCTTTGCTGGGAAGACTTGAGGGCTTGAAAGATGTGATTGAGCAAGTAAATCGGCAATTCAAAGACCCGGATATGACTACGTTCGTTTGTGTCTGCATCCCCGAGTTCTTGTCTCTCTATGAAACAGAGAGACTTGTTCAGGAACTCGCCAAGTTTGAGATTGACACGCATAACATTATCATCAACCAAGTACTATACGACGACGAAGATGTGGAGTCCAAGTTGCTCAGAGCAAGGATGCGGATGCAACAGAAGTATCTTGATCAGTTTTATATGCTATACGATGACTTTAATATCACAAAGCTTCCTCTGCTTCCGGAAGAGGTGACAGGGGTTGAAGCCTTAAAGGCGTTTTCACATAAGTTCTTGACGCCGTACCATCCTACCACTAGCAGGAGCAATGTAGAGGAGCTGGAGAGGAAAGTACACACATTGCGTTTGCAGTTAAAAACAGCTGAAGAAGAACTCGAACGGGTCAAGAGTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

353

Amino Acids

39.67

Weight (kDa)

4.73

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 19 - 208 5.2e-60 Anion-transporting ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0010938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01910 AT1G01910 AT1G01910 AT1G01910
fragaria_vesca FvH4_7g12910
malus_domestica MD02G1195500.v1.1 MD07G1125500.v1.1
prunus_persica Prupe.2G153000_v2.0.a1 Prupe.2G153000_v2.0.a1
pyrus_communis pycom02g15940 pycom07g11890
rosa_chinensis RchiOBHm_Chr1g0351991
rosa_laevigata RLG00000028370
rosa_multiflora Rmu_sc0007728.1_g000011
rosa_roxburghii Rroxscaffold_4G00303370
rosa_rugosa Rorug01G0223300
rosa_samantha Rh1BG207500 Rh1CG221700 Rh1DG234500
rosa_wichuraiana Rw1G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 889
AciI CCGC 4 cut(s) 5, 8, 445, 814
AclWI GGATC 3 cut(s) 135, 161, 257
AcoI YGGCCR 1 cut(s) 462
AcuI CTGAAG 1 cut(s) 1050
AcyI GRCGYC 1 cut(s) 944
AfaI GTAC 5 cut(s) 30, 295, 765, 950, 999
AfiI CCNNNNNNNGG 1 cut(s) 966
AgsI TTSAA 8 cut(s) 111, 230, 275, 379, 502, 601, 634, 911
AhdI GACNNNNNGTC 1 cut(s) 49
AjnI CCWGG 1 cut(s) 340
AloI GAACNNNNNNTCC 2 cut(s) 545, 577
AluBI AGCT 3 cut(s) 877, 985, 1028
AluI AGCT 3 cut(s) 877, 985, 1028
Alw26I GTCTC 4 cut(s) 57, 131, 690, 697
AlwI GGATC 3 cut(s) 135, 161, 257
Ama87I CYCGRG 1 cut(s) 674
Aor13HI TCCGGA 1 cut(s) 889
AoxI GGCC 1 cut(s) 462
ArsI GACNNNNNNTTYG 2 cut(s) 479, 511
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 1 cut(s) 641
AsuHPI GGTGA 1 cut(s) 910
AsuII TTCGAA 1 cut(s) 485
AvaI CYCGRG 1 cut(s) 674
AvaII GGWCC 1 cut(s) 43
BaeI ACNNNNGTAYC 2 cut(s) 812, 845
BbsI GAAGAC 2 cut(s) 101, 592
BccI CCATC 3 cut(s) 298, 302, 960
BceAI ACGGC 2 cut(s) 522, 931
BcgI CGANNNNNNTGC 2 cut(s) 605, 639
BciT130I CCWGG 1 cut(s) 342
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 1 cut(s) 641
BcoDI GTCTC 4 cut(s) 57, 131, 690, 697
BfaI CTAG 2 cut(s) 470, 963
BglI GCCNNNNNGGC 1 cut(s) 920
BisI GCNGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 7
BmcAI AGTACT 1 cut(s) 765
Bme1390I CCNGG 2 cut(s) 342, 641
Bme18I GGWCC 1 cut(s) 43
BmeRI GACNNNNNGTC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 674
BmgT120I GGNCC 1 cut(s) 43
BmrFI CCNGG 2 cut(s) 342, 641
BmsI GCATC 5 cut(s) 178, 562, 678, 801, 807
BpiI GAAGAC 2 cut(s) 101, 592
BpmI CTGGAG 1 cut(s) 1007
Bpu14I TTCGAA 1 cut(s) 485
BpuEI CTTGAG 1 cut(s) 611
BpuMI CCSGG 1 cut(s) 641
Bsa29I ATCGAT 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 944
BsaJI CCNNGG 1 cut(s) 252
BsaWI WCCGGW 1 cut(s) 889
BsaXI ACNNNNNCTCC 8 cut(s) 199, 229, 426, 456, 545, 575, 779, 809
Bsc4I CCNNNNNNNGG 1 cut(s) 966
Bse1I ACTGG 2 cut(s) 132, 436
Bse3DI GCAATG 2 cut(s) 979, 1004
BseAI TCCGGA 1 cut(s) 889
BseBI CCWGG 1 cut(s) 342
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 8 cut(s) 309, 313, 562, 577, 669, 816, 822, 952
BseLI CCNNNNNNNGG 1 cut(s) 966
BseMI GCAATG 2 cut(s) 979, 1004
BseMII CTCAG 3 cut(s) 360, 542, 814
BseNI ACTGG 2 cut(s) 132, 436
BseRI GAGGAG 1 cut(s) 995
BseYI CCCAGC 1 cut(s) 580
BshFI GGCC 1 cut(s) 464
BshVI ATCGAT 1 cut(s) 348
BsiHKCI CYCGRG 1 cut(s) 674
BsiSI CCGG 4 cut(s) 17, 461, 641, 890
BslFI GGGAC 1 cut(s) 492
BslI CCNNNNNNNGG 1 cut(s) 966
BsmAI GTCTC 4 cut(s) 57, 131, 690, 697
BsmFI GGGAC 1 cut(s) 492
BsnI GGCC 1 cut(s) 464
BsoBI CYCGRG 1 cut(s) 674
Bsp119I TTCGAA 1 cut(s) 485
Bsp13I TCCGGA 1 cut(s) 889
Bsp143I GATC 4 cut(s) 140, 166, 262, 835
Bsp19I CCATGG 1 cut(s) 252
BspACI CCGC 4 cut(s) 5, 8, 445, 814
BspANI GGCC 1 cut(s) 464
BspCNI CTCAG 3 cut(s) 361, 541, 813
BspDI ATCGAT 1 cut(s) 348
BspEI TCCGGA 1 cut(s) 889
BspPI GGATC 3 cut(s) 135, 161, 257
BspT104I TTCGAA 1 cut(s) 485
BsrDI GCAATG 2 cut(s) 979, 1004
BsrI ACTGG 2 cut(s) 132, 436
BssECI CCNNGG 1 cut(s) 252
BssMI GATC 4 cut(s) 140, 166, 262, 835
BssNI GRCGYC 1 cut(s) 944
BssT1I CCWWGG 1 cut(s) 252
Bst2UI CCWGG 1 cut(s) 342
Bst4CI ACNGT 3 cut(s) 28, 271, 456
Bst6I CTCTTC 2 cut(s) 497, 888
BstACI GRCGYC 1 cut(s) 944
BstBI TTCGAA 1 cut(s) 485
BstDEI CTNAG 4 cut(s) 182, 369, 528, 800
BstDSI CCRYGG 1 cut(s) 252
BstF5I GGATG 8 cut(s) 309, 313, 562, 577, 669, 816, 822, 952
BstKTI GATC 4 cut(s) 143, 169, 265, 838
BstMAI GTCTC 4 cut(s) 57, 131, 690, 697
BstMBI GATC 4 cut(s) 140, 166, 262, 835
BstMWI GCNNNNNNNGC 2 cut(s) 883, 920
BstNI CCWGG 1 cut(s) 342
BstNSI RCATGY 1 cut(s) 105
BstSCI CCNGG 2 cut(s) 340, 639
BstV2I GAAGAC 2 cut(s) 101, 592
BstX2I RGATCY 1 cut(s) 262
BstYI RGATCY 1 cut(s) 262
Bsu15I ATCGAT 1 cut(s) 348
BsuRI GGCC 1 cut(s) 464
BsuTUI ATCGAT 1 cut(s) 348
BtgI CCRYGG 1 cut(s) 252
BtsCI GGATG 8 cut(s) 309, 313, 562, 577, 669, 816, 822, 952
BtsI GCAGTG 1 cut(s) 420
BtsIMutI CAGTG 2 cut(s) 139, 420
Cfr13I GGNCC 1 cut(s) 43
ClaI ATCGAT 1 cut(s) 348
CseI GACGC 1 cut(s) 952
Csp6I GTAC 5 cut(s) 29, 294, 764, 949, 998
CviAII CATG 4 cut(s) 102, 253, 286, 550
CviQI GTAC 5 cut(s) 29, 294, 764, 949, 998
DdeI CTNAG 4 cut(s) 182, 369, 528, 800
DpnI GATC 4 cut(s) 142, 168, 264, 837
DpnII GATC 4 cut(s) 140, 166, 262, 835
DriI GACNNNNNGTC 1 cut(s) 49
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 2 cut(s) 497, 888
Eam1105I GACNNNNNGTC 1 cut(s) 49
EarI CTCTTC 2 cut(s) 497, 888
EciI GGCGGA 2 cut(s) 23, 434
Eco130I CCWWGG 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 43
Eco57I CTGAAG 1 cut(s) 1050
Eco88I CYCGRG 1 cut(s) 674
EcoRII CCWGG 1 cut(s) 340
EcoT14I CCWWGG 1 cut(s) 252
ErhI CCWWGG 1 cut(s) 252
FaeI CATG 4 cut(s) 105, 256, 289, 553
FalI AAGNNNNNCTT 2 cut(s) 39, 71
FaqI GGGAC 1 cut(s) 492
FatI CATG 4 cut(s) 101, 252, 285, 549
FbaI TGATCA 1 cut(s) 835
Fnu4HI GCNGC 1 cut(s) 6
FokI GGATG 8 cut(s) 316, 320, 569, 584, 656, 823, 829, 939
Fsp4HI GCNGC 1 cut(s) 6
FspBI CTAG 2 cut(s) 470, 963
GluI GCNGC 1 cut(s) 6
GsaI CCCAGC 1 cut(s) 584
GsuI CTGGAG 1 cut(s) 1007
HaeIII GGCC 1 cut(s) 464
HapII CCGG 4 cut(s) 17, 461, 641, 890
HgaI GACGC 1 cut(s) 952
Hin1I GRCGYC 1 cut(s) 944
Hin1II CATG 4 cut(s) 105, 256, 289, 553
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 5 cut(s) 50, 337, 345, 526, 788
HpaII CCGG 4 cut(s) 17, 461, 641, 890
HphI GGTGA 1 cut(s) 910
Hpy166II GTNNAC 1 cut(s) 1000
Hpy188I TCNGA 5 cut(s) 74, 322, 531, 566, 803
Hpy188III TCNNGA 6 cut(s) 41, 713, 833, 890, 940, 1051
Hpy8I GTNNAC 1 cut(s) 1000
Hpy99I CGWCG 3 cut(s) 74, 776, 779
HpyCH4III ACNGT 3 cut(s) 28, 271, 456
HpyCH4IV ACGT 1 cut(s) 653
HpyCH4V TGCA 5 cut(s) 105, 388, 669, 820, 1015
HpyF10VI GCNNNNNNNGC 2 cut(s) 883, 920
HpyF3I CTNAG 4 cut(s) 182, 369, 528, 800
HpySE526I ACGT 1 cut(s) 653
Hsp92I GRCGYC 1 cut(s) 944
Hsp92II CATG 4 cut(s) 105, 256, 289, 553
Kpn2I TCCGGA 1 cut(s) 889
Ksp22I TGATCA 1 cut(s) 835
Kzo9I GATC 4 cut(s) 140, 166, 262, 835
LmnI GCTCC 3 cut(s) 430, 969, 982
LweI GCATC 5 cut(s) 178, 562, 678, 801, 807
MaeI CTAG 2 cut(s) 470, 963
MaeII ACGT 1 cut(s) 653
MaeIII GTNAC 2 cut(s) 450, 898
MalI GATC 4 cut(s) 142, 168, 264, 837
MboI GATC 4 cut(s) 140, 166, 262, 835
MboII GAAGA 7 cut(s) 106, 514, 597, 791, 905, 1043, 1046
MflI RGATCY 1 cut(s) 262
MluCI AATT 3 cut(s) 111, 120, 629
MlyI GAGTC 3 cut(s) 59, 520, 797
MmeI TCCRAC 1 cut(s) 52
MroI TCCGGA 1 cut(s) 889
MseI TTAA 4 cut(s) 57, 864, 917, 1019
MspA1I CMGCKG 1 cut(s) 1028
MspI CCGG 4 cut(s) 17, 461, 641, 890
MspR9I CCNGG 2 cut(s) 342, 641
MvaI CCWGG 1 cut(s) 342
MwoI GCNNNNNNNGC 2 cut(s) 883, 920
NciI CCSGG 1 cut(s) 641
NcoI CCATGG 1 cut(s) 252
NdeII GATC 4 cut(s) 140, 166, 262, 835
NlaIII CATG 4 cut(s) 105, 256, 289, 553
NmuCI GTSAC 1 cut(s) 898
NspI RCATGY 1 cut(s) 105
NspV TTCGAA 1 cut(s) 485
PfeI GAWTC 2 cut(s) 337, 345
PfoI TCCNGGA 1 cut(s) 340
PkrI GCNGC 1 cut(s) 7
PleI GAGTC 3 cut(s) 58, 520, 796
PpsI GAGTC 3 cut(s) 58, 520, 796
Psp6I CCWGG 1 cut(s) 340
PspFI CCCAGC 1 cut(s) 580
PspGI CCWGG 1 cut(s) 340
PspPI GGNCC 1 cut(s) 43
PsuI RGATCY 1 cut(s) 262
PvuII CAGCTG 1 cut(s) 1028
RsaI GTAC 5 cut(s) 30, 295, 765, 950, 999
RsaNI GTAC 5 cut(s) 29, 294, 764, 949, 998
SaqAI TTAA 4 cut(s) 57, 864, 917, 1019
SatI GCNGC 1 cut(s) 6
Sau3AI GATC 4 cut(s) 140, 166, 262, 835
Sau96I GGNCC 1 cut(s) 43
ScaI AGTACT 1 cut(s) 765
SchI GAGTC 3 cut(s) 59, 520, 797
ScrFI CCNGG 2 cut(s) 342, 641
SetI ASST 9 cut(s) 79, 88, 235, 261, 656, 879, 900, 987, 1030
SfaNI GCATC 5 cut(s) 178, 562, 678, 801, 807
SfuI TTCGAA 1 cut(s) 485
SinI GGWCC 1 cut(s) 43
SmlI CTYRAG 1 cut(s) 590
SmoI CTYRAG 1 cut(s) 590
Sse9I AATT 3 cut(s) 111, 120, 629
SsiI CCGC 4 cut(s) 5, 8, 445, 814
SspMI CTAG 2 cut(s) 470, 963
StyD4I CCNGG 2 cut(s) 340, 639
StyI CCWWGG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 28, 271, 456
TaiI ACGT 1 cut(s) 656
TaqI TCGA 4 cut(s) 139, 348, 485, 1041
TasI AATT 3 cut(s) 111, 120, 629
TatI WGTACW 3 cut(s) 28, 763, 997
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 337, 345
Tru1I TTAA 4 cut(s) 57, 864, 917, 1019
Tru9I TTAA 4 cut(s) 57, 864, 917, 1019
TscAI CASTG 2 cut(s) 139, 427
TseFI GTSAC 1 cut(s) 898
Tsp45I GTSAC 1 cut(s) 898
TspDTI ATGAA 1 cut(s) 708
TspGWI ACGGA 1 cut(s) 136
TspRI CASTG 2 cut(s) 139, 427
VpaK11BI GGWCC 1 cut(s) 43
XceI RCATGY 1 cut(s) 105
XspI CTAG 2 cut(s) 470, 963
ZrmI AGTACT 1 cut(s) 765
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.