ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G01910 AT1G01910 AT1G01910 AT1G01910 |
| fragaria_vesca | FvH4_7g12910 |
| malus_domestica | MD02G1195500.v1.1 MD07G1125500.v1.1 |
| prunus_persica | Prupe.2G153000_v2.0.a1 Prupe.2G153000_v2.0.a1 |
| pyrus_communis | pycom02g15940 pycom07g11890 |
| rosa_chinensis | RchiOBHm_Chr1g0351991 |
| rosa_laevigata | RLG00000028370 |
| rosa_multiflora | Rmu_sc0007728.1_g000011 |
| rosa_roxburghii | Rroxscaffold_4G00303370 |
| rosa_rugosa | Rorug01G0223300 |
| rosa_samantha | Rh1BG207500 Rh1CG221700 Rh1DG234500 |
| rosa_wichuraiana | Rw1G020640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 400 |
| AciI | CCGC | 2 cut(s) | 81, 522 |
| AclWI | GGATC | 5 cut(s) | 112, 121, 141, 263, 276 |
| AcoI | YGGCCR | 1 cut(s) | 439 |
| AcsI | RAATTY | 4 cut(s) | 515, 566, 683, 731 |
| AfaI | GTAC | 3 cut(s) | 45, 771, 836 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 67, 400 |
| AflIII | ACRYGT | 2 cut(s) | 107, 852 |
| AgsI | TTSAA | 3 cut(s) | 508, 620, 1027 |
| AhlI | ACTAGT | 1 cut(s) | 712 |
| AjnI | CCWGG | 1 cut(s) | 346 |
| AluBI | AGCT | 4 cut(s) | 114, 369, 595, 883 |
| AluI | AGCT | 4 cut(s) | 114, 369, 595, 883 |
| Alw21I | GWGCWC | 1 cut(s) | 285 |
| Alw26I | GTCTC | 1 cut(s) | 703 |
| AlwI | GGATC | 5 cut(s) | 112, 121, 141, 263, 276 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 14 |
| AoxI | GGCC | 3 cut(s) | 360, 439, 523 |
| ApeKI | GCWGC | 3 cut(s) | 883, 892, 1018 |
| ApoI | RAATTY | 4 cut(s) | 515, 566, 683, 731 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 211, 243 |
| AspS9I | GGNCC | 1 cut(s) | 391 |
| AsuHPI | GGTGA | 5 cut(s) | 205, 235, 572, 718, 868 |
| AvaII | GGWCC | 1 cut(s) | 391 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 818, 851 |
| BalI | TGGCCA | 1 cut(s) | 441 |
| BamHI | GGATCC | 1 cut(s) | 268 |
| BbsI | GAAGAC | 1 cut(s) | 294 |
| Bbv12I | GWGCWC | 1 cut(s) | 285 |
| BbvI | GCAGC | 3 cut(s) | 870, 879, 1030 |
| BccI | CCATC | 2 cut(s) | 299, 475 |
| BciT130I | CCWGG | 1 cut(s) | 348 |
| BclI | TGATCA | 1 cut(s) | 841 |
| BcoDI | GTCTC | 1 cut(s) | 703 |
| BcuI | ACTAGT | 1 cut(s) | 712 |
| BfaI | CTAG | 3 cut(s) | 332, 713, 1046 |
| BisI | GCNGC | 5 cut(s) | 82, 523, 884, 893, 1019 |
| BlsI | GCNGC | 5 cut(s) | 83, 524, 885, 894, 1020 |
| BmcAI | AGTACT | 1 cut(s) | 771 |
| Bme1390I | CCNGG | 1 cut(s) | 348 |
| Bme18I | GGWCC | 1 cut(s) | 391 |
| BmgT120I | GGNCC | 1 cut(s) | 391 |
| BmiI | GGNNCC | 1 cut(s) | 270 |
| BmrFI | CCNGG | 1 cut(s) | 348 |
| BmsI | GCATC | 4 cut(s) | 184, 369, 568, 907 |
| BoxI | GACNNNNGTC | 1 cut(s) | 662 |
| BpiI | GAAGAC | 1 cut(s) | 294 |
| BpmI | CTGGAG | 2 cut(s) | 121, 617 |
| BpuEI | CTTGAG | 1 cut(s) | 47 |
| BsaJI | CCNNGG | 2 cut(s) | 258, 474 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 205, 235 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 67, 400 |
| Bse1I | ACTGG | 2 cut(s) | 51, 442 |
| Bse3DI | GCAATG | 1 cut(s) | 887 |
| BseBI | CCWGG | 1 cut(s) | 348 |
| BseDI | CCNNGG | 2 cut(s) | 258, 474 |
| BseGI | GGATG | 1 cut(s) | 583 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 67, 400 |
| BseMI | GCAATG | 1 cut(s) | 887 |
| BseMII | CTCAG | 3 cut(s) | 202, 548, 1046 |
| BseNI | ACTGG | 2 cut(s) | 51, 442 |
| BseXI | GCAGC | 3 cut(s) | 870, 879, 1030 |
| BsgI | GTGCAG | 2 cut(s) | 414, 737 |
| BshFI | GGCC | 3 cut(s) | 362, 441, 525 |
| BsiHKAI | GWGCWC | 1 cut(s) | 285 |
| BslFI | GGGAC | 1 cut(s) | 43 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 67, 400 |
| BsmAI | GTCTC | 1 cut(s) | 703 |
| BsmFI | GGGAC | 1 cut(s) | 43 |
| BsmI | GAATGC | 3 cut(s) | 675, 822, 828 |
| BsnI | GGCC | 3 cut(s) | 362, 441, 525 |
| Bsp1286I | GDGCHC | 1 cut(s) | 285 |
| Bsp143I | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| Bsp19I | CCATGG | 1 cut(s) | 258 |
| BspACI | CCGC | 2 cut(s) | 81, 522 |
| BspANI | GGCC | 3 cut(s) | 362, 441, 525 |
| BspCNI | CTCAG | 3 cut(s) | 201, 547, 1047 |
| BspLI | GGNNCC | 1 cut(s) | 270 |
| BspPI | GGATC | 5 cut(s) | 112, 121, 141, 263, 276 |
| BsrDI | GCAATG | 1 cut(s) | 887 |
| BsrI | ACTGG | 2 cut(s) | 51, 442 |
| BssECI | CCNNGG | 2 cut(s) | 258, 474 |
| BssMI | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| BssT1I | CCWWGG | 2 cut(s) | 258, 474 |
| Bst2UI | CCWGG | 1 cut(s) | 348 |
| Bst4CI | ACNGT | 1 cut(s) | 277 |
| Bst6I | CTCTTC | 2 cut(s) | 894, 981 |
| BstDEI | CTNAG | 4 cut(s) | 188, 534, 1013, 1055 |
| BstDSI | CCRYGG | 1 cut(s) | 258 |
| BstF5I | GGATG | 1 cut(s) | 583 |
| BstKTI | GATC | 7 cut(s) | 120, 129, 149, 162, 271, 331, 844 |
| BstMAI | GTCTC | 1 cut(s) | 703 |
| BstMBI | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 90, 889, 892, 926 |
| BstNI | CCWGG | 1 cut(s) | 348 |
| BstNSI | RCATGY | 2 cut(s) | 111, 856 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 662 |
| BstSCI | CCNGG | 1 cut(s) | 346 |
| BstV1I | GCAGC | 3 cut(s) | 870, 879, 1030 |
| BstV2I | GAAGAC | 1 cut(s) | 294 |
| BstX2I | RGATCY | 2 cut(s) | 146, 268 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 265 |
| BstYI | RGATCY | 2 cut(s) | 146, 268 |
| BsuRI | GGCC | 3 cut(s) | 362, 441, 525 |
| BtgI | CCRYGG | 1 cut(s) | 258 |
| BtgZI | GCGATG | 1 cut(s) | 207 |
| BtsCI | GGATG | 1 cut(s) | 583 |
| CaiI | CAGNNNCTG | 1 cut(s) | 14 |
| Cfr13I | GGNCC | 1 cut(s) | 391 |
| Csp6I | GTAC | 3 cut(s) | 44, 770, 835 |
| CviAII | CATG | 6 cut(s) | 108, 259, 292, 364, 604, 853 |
| CviQI | GTAC | 3 cut(s) | 44, 770, 835 |
| DdeI | CTNAG | 4 cut(s) | 188, 534, 1013, 1055 |
| DpnI | GATC | 7 cut(s) | 119, 128, 148, 161, 270, 330, 843 |
| DpnII | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| DraI | TTTAAA | 1 cut(s) | 640 |
| EaeI | YGGCCR | 1 cut(s) | 439 |
| Eam1104I | CTCTTC | 2 cut(s) | 894, 981 |
| EarI | CTCTTC | 2 cut(s) | 894, 981 |
| Eco130I | CCWWGG | 2 cut(s) | 258, 474 |
| Eco47I | GGWCC | 1 cut(s) | 391 |
| EcoRI | GAATTC | 1 cut(s) | 683 |
| EcoRII | CCWGG | 1 cut(s) | 346 |
| EcoT14I | CCWWGG | 2 cut(s) | 258, 474 |
| ErhI | CCWWGG | 2 cut(s) | 258, 474 |
| FaeI | CATG | 6 cut(s) | 111, 262, 295, 367, 607, 856 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 56, 88 |
| FaqI | GGGAC | 1 cut(s) | 43 |
| FatI | CATG | 6 cut(s) | 107, 258, 291, 363, 603, 852 |
| FbaI | TGATCA | 1 cut(s) | 841 |
| Fnu4HI | GCNGC | 5 cut(s) | 82, 523, 884, 893, 1019 |
| FokI | GGATG | 1 cut(s) | 590 |
| Fsp4HI | GCNGC | 5 cut(s) | 82, 523, 884, 893, 1019 |
| FspBI | CTAG | 3 cut(s) | 332, 713, 1046 |
| GluI | GCNGC | 5 cut(s) | 82, 523, 884, 893, 1019 |
| GsuI | CTGGAG | 2 cut(s) | 121, 617 |
| HaeIII | GGCC | 3 cut(s) | 362, 441, 525 |
| Hin1II | CATG | 6 cut(s) | 111, 262, 295, 367, 607, 856 |
| HincII | GTYRAC | 1 cut(s) | 235 |
| HindII | GTYRAC | 1 cut(s) | 235 |
| HinfI | GANTC | 4 cut(s) | 141, 220, 532, 794 |
| HpaI | GTTAAC | 1 cut(s) | 235 |
| HphI | GGTGA | 5 cut(s) | 205, 235, 572, 718, 868 |
| Hpy166II | GTNNAC | 3 cut(s) | 213, 235, 243 |
| Hpy188I | TCNGA | 5 cut(s) | 16, 146, 328, 537, 948 |
| Hpy188III | TCNNGA | 3 cut(s) | 124, 138, 680 |
| Hpy8I | GTNNAC | 3 cut(s) | 213, 235, 243 |
| HpyAV | CCTTC | 4 cut(s) | 140, 779, 972, 1066 |
| HpyCH4III | ACNGT | 1 cut(s) | 277 |
| HpyCH4IV | ACGT | 1 cut(s) | 42 |
| HpyCH4V | TGCA | 7 cut(s) | 374, 431, 460, 581, 675, 718, 826 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 90, 889, 892, 926 |
| HpyF3I | CTNAG | 4 cut(s) | 188, 534, 1013, 1055 |
| HpySE526I | ACGT | 1 cut(s) | 42 |
| Hsp92II | CATG | 6 cut(s) | 111, 262, 295, 367, 607, 856 |
| Ksp22I | TGATCA | 1 cut(s) | 841 |
| KspAI | GTTAAC | 1 cut(s) | 235 |
| Kzo9I | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| Lsp1109I | GCAGC | 3 cut(s) | 870, 879, 1030 |
| LweI | GCATC | 4 cut(s) | 184, 369, 568, 907 |
| MaeI | CTAG | 3 cut(s) | 332, 713, 1046 |
| MaeII | ACGT | 1 cut(s) | 42 |
| MaeIII | GTNAC | 3 cut(s) | 560, 904, 1006 |
| MalI | GATC | 7 cut(s) | 119, 128, 148, 161, 270, 330, 843 |
| MboI | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| MboII | GAAGA | 4 cut(s) | 299, 794, 911, 998 |
| MflI | RGATCY | 2 cut(s) | 146, 268 |
| MhlI | GDGCHC | 1 cut(s) | 285 |
| MlsI | TGGCCA | 1 cut(s) | 441 |
| MluCI | AATT | 8 cut(s) | 351, 386, 515, 566, 582, 635, 683, 731 |
| MluNI | TGGCCA | 1 cut(s) | 441 |
| MlyI | GAGTC | 3 cut(s) | 150, 214, 526 |
| Mox20I | TGGCCA | 1 cut(s) | 441 |
| MscI | TGGCCA | 1 cut(s) | 441 |
| MseI | TTAA | 5 cut(s) | 234, 639, 762, 807, 870 |
| MslI | CAYNNNNRTG | 1 cut(s) | 263 |
| Msp20I | TGGCCA | 1 cut(s) | 441 |
| MspR9I | CCNGG | 1 cut(s) | 348 |
| Mva1269I | GAATGC | 3 cut(s) | 675, 822, 828 |
| MvaI | CCWGG | 1 cut(s) | 348 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 90, 889, 892, 926 |
| NcoI | CCATGG | 1 cut(s) | 258 |
| NdeII | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| NlaIII | CATG | 6 cut(s) | 111, 262, 295, 367, 607, 856 |
| NlaIV | GGNNCC | 1 cut(s) | 270 |
| NmuCI | GTSAC | 2 cut(s) | 560, 1006 |
| NspI | RCATGY | 2 cut(s) | 111, 856 |
| PciI | ACATGT | 2 cut(s) | 107, 852 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 122 |
| PctI | GAATGC | 3 cut(s) | 675, 822, 828 |
| PfeI | GAWTC | 1 cut(s) | 794 |
| PflFI | GACNNNGTC | 1 cut(s) | 10 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 400 |
| PfoI | TCCNGGA | 1 cut(s) | 346 |
| PkrI | GCNGC | 5 cut(s) | 83, 524, 885, 894, 1020 |
| PleI | GAGTC | 3 cut(s) | 149, 214, 526 |
| PpsI | GAGTC | 3 cut(s) | 149, 214, 526 |
| PscI | ACATGT | 2 cut(s) | 107, 852 |
| PshAI | GACNNNNGTC | 1 cut(s) | 662 |
| Psp6I | CCWGG | 1 cut(s) | 346 |
| PspGI | CCWGG | 1 cut(s) | 346 |
| PspN4I | GGNNCC | 1 cut(s) | 270 |
| PspPI | GGNCC | 1 cut(s) | 391 |
| PstNI | CAGNNNCTG | 1 cut(s) | 14 |
| PsuI | RGATCY | 2 cut(s) | 146, 268 |
| PsyI | GACNNNGTC | 1 cut(s) | 10 |
| RsaI | GTAC | 3 cut(s) | 45, 771, 836 |
| RsaNI | GTAC | 3 cut(s) | 44, 770, 835 |
| RseI | CAYNNNNRTG | 1 cut(s) | 263 |
| SaqAI | TTAA | 5 cut(s) | 234, 639, 762, 807, 870 |
| SatI | GCNGC | 5 cut(s) | 82, 523, 884, 893, 1019 |
| Sau3AI | GATC | 7 cut(s) | 117, 126, 146, 159, 268, 328, 841 |
| Sau96I | GGNCC | 1 cut(s) | 391 |
| ScaI | AGTACT | 1 cut(s) | 771 |
| SchI | GAGTC | 3 cut(s) | 150, 214, 526 |
| ScrFI | CCNGG | 1 cut(s) | 348 |
| SduI | GDGCHC | 1 cut(s) | 285 |
| SfaNI | GCATC | 4 cut(s) | 184, 369, 568, 907 |
| SinI | GGWCC | 1 cut(s) | 391 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 263 |
| SmlI | CTYRAG | 1 cut(s) | 62 |
| SmoI | CTYRAG | 1 cut(s) | 62 |
| SpeI | ACTAGT | 1 cut(s) | 712 |
| Sse9I | AATT | 8 cut(s) | 351, 386, 515, 566, 582, 635, 683, 731 |
| SsiI | CCGC | 2 cut(s) | 81, 522 |
| SspMI | CTAG | 3 cut(s) | 332, 713, 1046 |
| StyD4I | CCNGG | 1 cut(s) | 346 |
| StyI | CCWWGG | 2 cut(s) | 258, 474 |
| TaaI | ACNGT | 1 cut(s) | 277 |
| TaiI | ACGT | 1 cut(s) | 45 |
| TaqI | TCGA | 4 cut(s) | 116, 125, 158, 631 |
| TasI | AATT | 8 cut(s) | 351, 386, 515, 566, 582, 635, 683, 731 |
| TatI | WGTACW | 1 cut(s) | 769 |
| TauI | GCSGC | 2 cut(s) | 84, 525 |
| TfiI | GAWTC | 1 cut(s) | 794 |
| Tru1I | TTAA | 5 cut(s) | 234, 639, 762, 807, 870 |
| Tru9I | TTAA | 5 cut(s) | 234, 639, 762, 807, 870 |
| TseFI | GTSAC | 2 cut(s) | 560, 1006 |
| TseI | GCWGC | 3 cut(s) | 883, 892, 1018 |
| Tsp45I | GTSAC | 2 cut(s) | 560, 1006 |
| TspDTI | ATGAA | 3 cut(s) | 620, 714, 795 |
| TspGWI | ACGGA | 1 cut(s) | 142 |
| Tth111I | GACNNNGTC | 1 cut(s) | 10 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 400 |
| VpaK11BI | GGWCC | 1 cut(s) | 391 |
| XapI | RAATTY | 4 cut(s) | 515, 566, 683, 731 |
| XceI | RCATGY | 2 cut(s) | 111, 856 |
| XspI | CTAG | 3 cut(s) | 332, 713, 1046 |
| ZrmI | AGTACT | 1 cut(s) | 771 |