ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G01910 AT1G01910 AT1G01910 AT1G01910 |
| fragaria_vesca | FvH4_7g12910 |
| malus_domestica | MD02G1195500.v1.1 MD07G1125500.v1.1 |
| prunus_persica | Prupe.2G153000_v2.0.a1 Prupe.2G153000_v2.0.a1 |
| pyrus_communis | pycom02g15940 pycom07g11890 |
| rosa_chinensis | RchiOBHm_Chr1g0351991 |
| rosa_laevigata | RLG00000028370 |
| rosa_multiflora | Rmu_sc0007728.1_g000011 |
| rosa_roxburghii | Rroxscaffold_4G00303370 |
| rosa_rugosa | Rorug01G0223300 |
| rosa_samantha | Rh1BG207500 Rh1CG221700 Rh1DG234500 |
| rosa_wichuraiana | Rw1G020640 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 4 cut(s) | 78, 294, 519, 541 |
| AclWI | GGATC | 4 cut(s) | 118, 138, 260, 273 |
| AcsI | RAATTY | 4 cut(s) | 512, 563, 680, 728 |
| AcuI | CTGAAG | 2 cut(s) | 17, 915 |
| AfaI | GTAC | 3 cut(s) | 42, 768, 833 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 63, 64, 1005 |
| AflIII | ACRYGT | 2 cut(s) | 104, 849 |
| AgsI | TTSAA | 4 cut(s) | 392, 505, 637, 1018 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 790 |
| AjiI | CACGTC | 1 cut(s) | 105 |
| AjnI | CCWGG | 1 cut(s) | 343 |
| AluBI | AGCT | 7 cut(s) | 111, 328, 366, 592, 880, 917, 1036 |
| AluI | AGCT | 7 cut(s) | 111, 328, 366, 592, 880, 917, 1036 |
| Alw26I | GTCTC | 3 cut(s) | 47, 693, 700 |
| AlwI | GGATC | 4 cut(s) | 118, 138, 260, 273 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 11 |
| AoxI | GGCC | 2 cut(s) | 357, 1065 |
| ApeKI | GCWGC | 3 cut(s) | 5, 889, 1009 |
| ApoI | RAATTY | 4 cut(s) | 512, 563, 680, 728 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 208, 240, 482, 514 |
| AsuC2I | CCSGG | 1 cut(s) | 644 |
| AsuHPI | GGTGA | 1 cut(s) | 202 |
| AsuNHI | GCTAGC | 1 cut(s) | 328 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 815, 848 |
| BamHI | GGATCC | 1 cut(s) | 265 |
| BbsI | GAAGAC | 3 cut(s) | 61, 291, 789 |
| BbvI | GCAGC | 3 cut(s) | 17, 876, 1021 |
| BccI | CCATC | 1 cut(s) | 472 |
| BciT130I | CCWGG | 1 cut(s) | 345 |
| BciVI | GTATCC | 1 cut(s) | 1006 |
| BcnI | CCSGG | 1 cut(s) | 644 |
| BcoDI | GTCTC | 3 cut(s) | 47, 693, 700 |
| BfaI | CTAG | 2 cut(s) | 329, 1037 |
| BfuI | GTATCC | 1 cut(s) | 1006 |
| BisI | GCNGC | 4 cut(s) | 6, 79, 890, 1010 |
| BlsI | GCNGC | 4 cut(s) | 7, 80, 891, 1011 |
| BmcAI | AGTACT | 1 cut(s) | 768 |
| Bme1390I | CCNGG | 2 cut(s) | 345, 644 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 790 |
| BmgBI | CACGTC | 1 cut(s) | 105 |
| BmiI | GGNNCC | 2 cut(s) | 239, 267 |
| BmrFI | CCNGG | 2 cut(s) | 345, 644 |
| BmsI | GCATC | 2 cut(s) | 181, 565 |
| BmtI | GCTAGC | 1 cut(s) | 332 |
| BpiI | GAAGAC | 3 cut(s) | 61, 291, 789 |
| BpmI | CTGGAG | 2 cut(s) | 65, 927 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1004 |
| BpuEI | CTTGAG | 2 cut(s) | 44, 614 |
| BpuMI | CCSGG | 1 cut(s) | 644 |
| Bsa29I | ATCGAT | 1 cut(s) | 122 |
| BsaI | GGTCTC | 1 cut(s) | 47 |
| BsaJI | CCNNGG | 2 cut(s) | 255, 344 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 202, 232 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 63, 64, 1005 |
| Bse1I | ACTGG | 6 cut(s) | 48, 135, 439, 714, 841, 910 |
| Bse3DI | GCAATG | 1 cut(s) | 884 |
| BseBI | CCWGG | 1 cut(s) | 345 |
| BseCI | ATCGAT | 1 cut(s) | 122 |
| BseDI | CCNNGG | 2 cut(s) | 255, 344 |
| BseGI | GGATG | 1 cut(s) | 580 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 63, 64, 1005 |
| BseMI | GCAATG | 1 cut(s) | 884 |
| BseMII | CTCAG | 1 cut(s) | 199 |
| BseNI | ACTGG | 6 cut(s) | 48, 135, 439, 714, 841, 910 |
| BseXI | GCAGC | 3 cut(s) | 17, 876, 1021 |
| BsgI | GTGCAG | 2 cut(s) | 411, 734 |
| BshFI | GGCC | 2 cut(s) | 359, 1067 |
| BshVI | ATCGAT | 1 cut(s) | 122 |
| BsiSI | CCGG | 1 cut(s) | 644 |
| BslFI | GGGAC | 1 cut(s) | 40 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 63, 64, 1005 |
| BsmAI | GTCTC | 3 cut(s) | 47, 693, 700 |
| BsmFI | GGGAC | 1 cut(s) | 40 |
| BsmI | GAATGC | 3 cut(s) | 672, 819, 825 |
| BsnI | GGCC | 2 cut(s) | 359, 1067 |
| Bso31I | GGTCTC | 1 cut(s) | 47 |
| Bsp143I | GATC | 3 cut(s) | 123, 143, 265 |
| Bsp19I | CCATGG | 1 cut(s) | 255 |
| BspACI | CCGC | 4 cut(s) | 78, 294, 519, 541 |
| BspANI | GGCC | 2 cut(s) | 359, 1067 |
| BspCNI | CTCAG | 1 cut(s) | 198 |
| BspDI | ATCGAT | 1 cut(s) | 122 |
| BspLI | GGNNCC | 2 cut(s) | 239, 267 |
| BspOI | GCTAGC | 1 cut(s) | 332 |
| BspPI | GGATC | 4 cut(s) | 118, 138, 260, 273 |
| BspTNI | GGTCTC | 1 cut(s) | 47 |
| BsrDI | GCAATG | 1 cut(s) | 884 |
| BsrI | ACTGG | 6 cut(s) | 48, 135, 439, 714, 841, 910 |
| BssECI | CCNNGG | 2 cut(s) | 255, 344 |
| BssMI | GATC | 3 cut(s) | 123, 143, 265 |
| BssT1I | CCWWGG | 1 cut(s) | 255 |
| Bst2UI | CCWGG | 1 cut(s) | 345 |
| Bst4CI | ACNGT | 3 cut(s) | 274, 951, 973 |
| Bst6I | CTCTTC | 2 cut(s) | 36, 891 |
| BstC8I | GCNNGC | 1 cut(s) | 330 |
| BstDEI | CTNAG | 3 cut(s) | 185, 1004, 1046 |
| BstDSI | CCRYGG | 1 cut(s) | 255 |
| BstF5I | GGATG | 1 cut(s) | 580 |
| BstKTI | GATC | 3 cut(s) | 126, 146, 268 |
| BstMAI | GTCTC | 3 cut(s) | 47, 693, 700 |
| BstMBI | GATC | 3 cut(s) | 123, 143, 265 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 886, 923, 1009 |
| BstNI | CCWGG | 1 cut(s) | 345 |
| BstNSI | RCATGY | 1 cut(s) | 853 |
| BstSCI | CCNGG | 2 cut(s) | 343, 642 |
| BstV1I | GCAGC | 3 cut(s) | 17, 876, 1021 |
| BstV2I | GAAGAC | 3 cut(s) | 61, 291, 789 |
| BstX2I | RGATCY | 2 cut(s) | 143, 265 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 262, 973 |
| BstYI | RGATCY | 2 cut(s) | 143, 265 |
| Bsu15I | ATCGAT | 1 cut(s) | 122 |
| BsuI | GTATCC | 1 cut(s) | 1006 |
| BsuRI | GGCC | 2 cut(s) | 359, 1067 |
| BsuTUI | ATCGAT | 1 cut(s) | 122 |
| BtgI | CCRYGG | 1 cut(s) | 255 |
| BtgZI | GCGATG | 1 cut(s) | 204 |
| BtrI | CACGTC | 1 cut(s) | 105 |
| BtsCI | GGATG | 1 cut(s) | 580 |
| BtsI | GCAGTG | 1 cut(s) | 423 |
| BtsIMutI | CAGTG | 3 cut(s) | 142, 423, 978 |
| Cac8I | GCNNGC | 1 cut(s) | 330 |
| CaiI | CAGNNNCTG | 1 cut(s) | 11 |
| ClaI | ATCGAT | 1 cut(s) | 122 |
| Csp6I | GTAC | 3 cut(s) | 41, 767, 832 |
| CviAII | CATG | 5 cut(s) | 256, 281, 361, 601, 850 |
| CviQI | GTAC | 3 cut(s) | 41, 767, 832 |
| DdeI | CTNAG | 3 cut(s) | 185, 1004, 1046 |
| DpnI | GATC | 3 cut(s) | 125, 145, 267 |
| DpnII | GATC | 3 cut(s) | 123, 143, 265 |
| DriI | GACNNNNNGTC | 1 cut(s) | 790 |
| Eam1104I | CTCTTC | 2 cut(s) | 36, 891 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 790 |
| EarI | CTCTTC | 2 cut(s) | 36, 891 |
| EciI | GGCGGA | 1 cut(s) | 309 |
| Eco130I | CCWWGG | 1 cut(s) | 255 |
| Eco147I | AGGCCT | 1 cut(s) | 1067 |
| Eco31I | GGTCTC | 1 cut(s) | 47 |
| Eco57I | CTGAAG | 2 cut(s) | 17, 915 |
| EcoRI | GAATTC | 1 cut(s) | 680 |
| EcoRII | CCWGG | 1 cut(s) | 343 |
| EcoT14I | CCWWGG | 1 cut(s) | 255 |
| ErhI | CCWWGG | 1 cut(s) | 255 |
| FaeI | CATG | 5 cut(s) | 259, 284, 364, 604, 853 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 15, 47, 53, 85 |
| FaqI | GGGAC | 1 cut(s) | 40 |
| FatI | CATG | 5 cut(s) | 255, 280, 360, 600, 849 |
| FauI | CCCGC | 1 cut(s) | 548 |
| Fnu4HI | GCNGC | 4 cut(s) | 6, 79, 890, 1010 |
| FokI | GGATG | 1 cut(s) | 587 |
| Fsp4HI | GCNGC | 4 cut(s) | 6, 79, 890, 1010 |
| FspBI | CTAG | 2 cut(s) | 329, 1037 |
| GluI | GCNGC | 4 cut(s) | 6, 79, 890, 1010 |
| GsuI | CTGGAG | 2 cut(s) | 65, 927 |
| HaeIII | GGCC | 2 cut(s) | 359, 1067 |
| HapII | CCGG | 1 cut(s) | 644 |
| Hin1II | CATG | 5 cut(s) | 259, 284, 364, 604, 853 |
| HindIII | AAGCTT | 1 cut(s) | 590 |
| HinfI | GANTC | 5 cut(s) | 28, 217, 529, 785, 977 |
| HpaII | CCGG | 1 cut(s) | 644 |
| HphI | GGTGA | 1 cut(s) | 202 |
| Hpy166II | GTNNAC | 1 cut(s) | 210 |
| Hpy188I | TCNGA | 6 cut(s) | 13, 36, 325, 534, 921, 945 |
| Hpy188III | TCNNGA | 1 cut(s) | 677 |
| Hpy8I | GTNNAC | 1 cut(s) | 210 |
| Hpy99I | CGWCG | 1 cut(s) | 106 |
| HpyAV | CCTTC | 3 cut(s) | 17, 137, 969 |
| HpyCH4III | ACNGT | 3 cut(s) | 274, 951, 973 |
| HpyCH4IV | ACGT | 2 cut(s) | 39, 104 |
| HpyCH4V | TGCA | 7 cut(s) | 371, 428, 457, 488, 672, 715, 823 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 886, 923, 1009 |
| HpyF3I | CTNAG | 3 cut(s) | 185, 1004, 1046 |
| HpySE526I | ACGT | 2 cut(s) | 39, 104 |
| Hsp92II | CATG | 5 cut(s) | 259, 284, 364, 604, 853 |
| Kzo9I | GATC | 3 cut(s) | 123, 143, 265 |
| LmnI | GCTCC | 3 cut(s) | 46, 325, 885 |
| Lsp1109I | GCAGC | 3 cut(s) | 17, 876, 1021 |
| LweI | GCATC | 2 cut(s) | 181, 565 |
| MaeI | CTAG | 2 cut(s) | 329, 1037 |
| MaeII | ACGT | 2 cut(s) | 39, 104 |
| MaeIII | GTNAC | 1 cut(s) | 901 |
| MalI | GATC | 3 cut(s) | 125, 145, 267 |
| MboI | GATC | 3 cut(s) | 123, 143, 265 |
| MboII | GAAGA | 6 cut(s) | 23, 61, 296, 791, 794, 908 |
| MflI | RGATCY | 2 cut(s) | 143, 265 |
| MluCI | AATT | 7 cut(s) | 383, 512, 563, 632, 680, 728, 797 |
| MlyI | GAGTC | 5 cut(s) | 22, 211, 538, 779, 986 |
| MnlI | CCTC | 8 cut(s) | 19, 68, 157, 349, 554, 565, 589, 892 |
| MseI | TTAA | 7 cut(s) | 231, 380, 524, 624, 759, 804, 867 |
| MslI | CAYNNNNRTG | 1 cut(s) | 260 |
| MspI | CCGG | 1 cut(s) | 644 |
| MspR9I | CCNGG | 2 cut(s) | 345, 644 |
| Mva1269I | GAATGC | 3 cut(s) | 672, 819, 825 |
| MvaI | CCWGG | 1 cut(s) | 345 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 886, 923, 1009 |
| NciI | CCSGG | 1 cut(s) | 644 |
| NcoI | CCATGG | 1 cut(s) | 255 |
| NdeII | GATC | 3 cut(s) | 123, 143, 265 |
| NheI | GCTAGC | 1 cut(s) | 328 |
| NlaIII | CATG | 5 cut(s) | 259, 284, 364, 604, 853 |
| NlaIV | GGNNCC | 2 cut(s) | 239, 267 |
| NspI | RCATGY | 1 cut(s) | 853 |
| PceI | AGGCCT | 1 cut(s) | 1067 |
| PciI | ACATGT | 1 cut(s) | 849 |
| PctI | GAATGC | 3 cut(s) | 672, 819, 825 |
| PkrI | GCNGC | 4 cut(s) | 7, 80, 891, 1011 |
| PleI | GAGTC | 5 cut(s) | 22, 211, 537, 779, 985 |
| PpsI | GAGTC | 5 cut(s) | 22, 211, 537, 779, 985 |
| PscI | ACATGT | 1 cut(s) | 849 |
| Psp6I | CCWGG | 1 cut(s) | 343 |
| PspGI | CCWGG | 1 cut(s) | 343 |
| PspN4I | GGNNCC | 2 cut(s) | 239, 267 |
| PstNI | CAGNNNCTG | 1 cut(s) | 11 |
| PsuI | RGATCY | 2 cut(s) | 143, 265 |
| RsaI | GTAC | 3 cut(s) | 42, 768, 833 |
| RsaNI | GTAC | 3 cut(s) | 41, 767, 832 |
| RseI | CAYNNNNRTG | 1 cut(s) | 260 |
| SaqAI | TTAA | 7 cut(s) | 231, 380, 524, 624, 759, 804, 867 |
| SatI | GCNGC | 4 cut(s) | 6, 79, 890, 1010 |
| Sau3AI | GATC | 3 cut(s) | 123, 143, 265 |
| ScaI | AGTACT | 1 cut(s) | 768 |
| SchI | GAGTC | 5 cut(s) | 22, 211, 538, 779, 986 |
| ScrFI | CCNGG | 2 cut(s) | 345, 644 |
| SfaNI | GCATC | 2 cut(s) | 181, 565 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 260 |
| SmlI | CTYRAG | 2 cut(s) | 59, 593 |
| SmoI | CTYRAG | 2 cut(s) | 59, 593 |
| Sse9I | AATT | 7 cut(s) | 383, 512, 563, 632, 680, 728, 797 |
| SseBI | AGGCCT | 1 cut(s) | 1067 |
| SsiI | CCGC | 4 cut(s) | 78, 294, 519, 541 |
| SspI | AATATT | 1 cut(s) | 751 |
| SspMI | CTAG | 2 cut(s) | 329, 1037 |
| StuI | AGGCCT | 1 cut(s) | 1067 |
| StyD4I | CCNGG | 2 cut(s) | 343, 642 |
| StyI | CCWWGG | 1 cut(s) | 255 |
| TaaI | ACNGT | 3 cut(s) | 274, 951, 973 |
| TaiI | ACGT | 2 cut(s) | 42, 107 |
| TaqI | TCGA | 2 cut(s) | 122, 467 |
| TasI | AATT | 7 cut(s) | 383, 512, 563, 632, 680, 728, 797 |
| TatI | WGTACW | 1 cut(s) | 766 |
| TauI | GCSGC | 1 cut(s) | 81 |
| Tru1I | TTAA | 7 cut(s) | 231, 380, 524, 624, 759, 804, 867 |
| Tru9I | TTAA | 7 cut(s) | 231, 380, 524, 624, 759, 804, 867 |
| TscAI | CASTG | 3 cut(s) | 142, 430, 978 |
| TseI | GCWGC | 3 cut(s) | 5, 889, 1009 |
| TspDTI | ATGAA | 5 cut(s) | 297, 576, 617, 711, 792 |
| TspRI | CASTG | 3 cut(s) | 142, 430, 978 |
| XapI | RAATTY | 4 cut(s) | 512, 563, 680, 728 |
| XceI | RCATGY | 1 cut(s) | 853 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 970 |
| XspI | CTAG | 2 cut(s) | 329, 1037 |
| ZrmI | AGTACT | 1 cut(s) | 768 |