FvH4_7g12910

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0010938
Physical Location & Seq
Reverse (-)
11667414 .. 11671834
4421 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g12910.t2

Sequence Viewer

Length: 1071 bp
ATGGCAGGCTCTGATTTACCAGAAGGGACCCTGCAGAACGTACTGGAGCAGGAGACCCTCAAGTGGGTCTTCGTTGGCGGCAAAGGTGGTGTTGGCAAAACGACGTGTAGTTCAATCCTCTCGATCCTTCTCGCCAGTGTCAGATCCTCTGTTTTGATCATCTCCACTGACCCTGCTCACAATCTCAGCGACGCTTTTCAGCAAAAGTTTACCAAGACTCCTACTTTGGTTAATGGGTTCCCCAATTTGTATGCTATGGAAGTAGATCCTACTGTTGAGCATGAAGACATTGGCGGAGAGAATGGAATGGATAGTTTGGTTTCTGAGCTAGCAAATGCTATTCCAGGGATTGATGAGGCAATGAGCTTTGCAGAGATGTTAAAATTGGTTCAAACAATGGACTATTCTGTAATTGTATTTGACACTGCACCAACTGGACATACGCTTCGGCTGTTGCAGTTTCCATCAACATTAGAGAAAGGTCTTGCAAAAGTGATGTCTTTGAAAAATAAATTTGGCGGTCTAATGAGTCAGATGACCCGCATGTTTGGTATGGATGATGAATTTGGAGAGGATGCGATTTTAGGAAAGCTTGAGGGGATGAAAGAAGTAATAGAGCAAGTTAATAAGCAATTCAAAGACCCAGACTTGACAACTTTCGTTTGTGTTTGCATTCCTGAATTCCTCTCTCTCTATGAAACAGAGAGATTGGTGCAGGAACTCGCCAAATTTGAGATAGATACGCATAATATTATCATTAACCAAGTACTTTATGATGAAGAAGACTCGCAGTCCAAATTACTCAAAGCAAGAATGCGAATGCAACAAAAGTACCTTGACCAGTTCTACATGTTGTATGATGACTTTAACATAACAAAGCTCCCATTGCTACCTGAAGAGGTTACTGGAGTGGAAGCTCTGAAAGCATTTTCACGCCATTTTCTGACACCGTATCAACCTTCCACCAGCACGGTGGAATCGTTAGAGCAAAGGGTGTCTGCGCTAAGGCAGCAGTTGAAAGACACGGAAGCAGAGCTAGAAAGACTAAGAAAAGGAAAGGAAAAGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

357

Amino Acids

39.91

Weight (kDa)

4.79

Isoelectric Point (pI)

43.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ArsA_ATPase PF02374 4 - 211 3.2e-72 Anion-transporting ATPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010938)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G01910 AT1G01910 AT1G01910 AT1G01910
fragaria_vesca FvH4_7g12910
malus_domestica MD02G1195500.v1.1 MD07G1125500.v1.1
prunus_persica Prupe.2G153000_v2.0.a1 Prupe.2G153000_v2.0.a1
pyrus_communis pycom02g15940 pycom07g11890
rosa_chinensis RchiOBHm_Chr1g0351991
rosa_laevigata RLG00000028370
rosa_multiflora Rmu_sc0007728.1_g000011
rosa_roxburghii Rroxscaffold_4G00303370
rosa_rugosa Rorug01G0223300
rosa_samantha Rh1BG207500 Rh1CG221700 Rh1DG234500
rosa_wichuraiana Rw1G020640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 78, 294, 519, 541
AclWI GGATC 3 cut(s) 118, 138, 260
AcsI RAATTY 4 cut(s) 512, 563, 680, 728
AcuI CTGAAG 1 cut(s) 915
AfaI GTAC 3 cut(s) 42, 768, 833
AfiI CCNNNNNNNGG 2 cut(s) 63, 64
AflIII ACRYGT 2 cut(s) 104, 849
AgsI TTSAA 5 cut(s) 114, 392, 505, 637, 1018
AhdI GACNNNNNGTC 1 cut(s) 790
AjiI CACGTC 1 cut(s) 105
AjnI CCWGG 1 cut(s) 343
AluBI AGCT 6 cut(s) 328, 366, 592, 880, 917, 1036
AluI AGCT 6 cut(s) 328, 366, 592, 880, 917, 1036
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 3 cut(s) 118, 138, 260
AlwNI CAGNNNCTG 1 cut(s) 11
AoxI GGCC 1 cut(s) 1065
ApeKI GCWGC 1 cut(s) 1009
ApoI RAATTY 4 cut(s) 512, 563, 680, 728
ArsI GACNNNNNNTTYG 6 cut(s) 208, 240, 429, 461, 482, 514
AspLEI GCGC 1 cut(s) 1003
AspS9I GGNCC 1 cut(s) 27
AsuNHI GCTAGC 1 cut(s) 328
AvaII GGWCC 1 cut(s) 27
BaeI ACNNNNGTAYC 2 cut(s) 815, 848
BbsI GAAGAC 3 cut(s) 61, 291, 789
BbvI GCAGC 1 cut(s) 1021
BccI CCATC 1 cut(s) 472
BciT130I CCWGG 1 cut(s) 345
BclI TGATCA 1 cut(s) 156
BcoDI GTCTC 1 cut(s) 47
BfaI CTAG 2 cut(s) 329, 1037
BfmI CTRYAG 1 cut(s) 32
BisI GCNGC 2 cut(s) 79, 1010
BlsI GCNGC 2 cut(s) 80, 1011
BmcAI AGTACT 1 cut(s) 768
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 27
BmeRI GACNNNNNGTC 1 cut(s) 790
BmgBI CACGTC 1 cut(s) 105
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 28, 29, 239
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 565
BmtI GCTAGC 1 cut(s) 332
BpiI GAAGAC 3 cut(s) 61, 291, 789
BpmI CTGGAG 2 cut(s) 65, 927
Bpu10I CCTNAGC 1 cut(s) 1004
BpuEI CTTGAG 2 cut(s) 44, 614
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 1 cut(s) 344
BsaXI ACNNNNNCTCC 2 cut(s) 202, 232
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 64
Bse1I ACTGG 5 cut(s) 48, 135, 439, 841, 910
Bse3DI GCAATG 2 cut(s) 366, 884
BseBI CCWGG 1 cut(s) 345
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 3 cut(s) 562, 580, 606
BseLI CCNNNNNNNGG 2 cut(s) 63, 64
BseMI GCAATG 2 cut(s) 366, 884
BseMII CTCAG 2 cut(s) 199, 315
BseNI ACTGG 5 cut(s) 48, 135, 439, 841, 910
BseXI GCAGC 1 cut(s) 1021
BsgI GTGCAG 2 cut(s) 411, 734
BshFI GGCC 1 cut(s) 1067
BslFI GGGAC 1 cut(s) 40
BslI CCNNNNNNNGG 2 cut(s) 63, 64
BsmAI GTCTC 1 cut(s) 47
BsmFI GGGAC 1 cut(s) 40
BsmI GAATGC 3 cut(s) 672, 819, 825
BsnI GGCC 1 cut(s) 1067
Bso31I GGTCTC 1 cut(s) 47
Bsp143I GATC 4 cut(s) 123, 143, 156, 265
BspACI CCGC 4 cut(s) 78, 294, 519, 541
BspANI GGCC 1 cut(s) 1067
BspCNI CTCAG 2 cut(s) 198, 316
BspLI GGNNCC 3 cut(s) 28, 29, 239
BspMAI CTGCAG 1 cut(s) 36
BspOI GCTAGC 1 cut(s) 332
BspPI GGATC 3 cut(s) 118, 138, 260
BspTNI GGTCTC 1 cut(s) 47
BsrDI GCAATG 2 cut(s) 366, 884
BsrI ACTGG 5 cut(s) 48, 135, 439, 841, 910
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 4 cut(s) 123, 143, 156, 265
Bst2UI CCWGG 1 cut(s) 345
Bst4CI ACNGT 3 cut(s) 274, 951, 973
Bst6I CTCTTC 1 cut(s) 891
BstC8I GCNNGC 2 cut(s) 7, 330
BstDEI CTNAG 4 cut(s) 185, 324, 1004, 1046
BstF5I GGATG 3 cut(s) 562, 580, 606
BstHHI GCGC 1 cut(s) 1003
BstKTI GATC 4 cut(s) 126, 146, 159, 268
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 4 cut(s) 123, 143, 156, 265
BstMWI GCNNNNNNNGC 3 cut(s) 886, 923, 1009
BstNI CCWGG 1 cut(s) 345
BstNSI RCATGY 2 cut(s) 547, 853
BstSCI CCNGG 1 cut(s) 343
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 1021
BstV2I GAAGAC 3 cut(s) 61, 291, 789
BstX2I RGATCY 2 cut(s) 143, 265
BstXI CCANNNNNNTGG 1 cut(s) 973
BstYI RGATCY 2 cut(s) 143, 265
BsuRI GGCC 1 cut(s) 1067
BtrI CACGTC 1 cut(s) 105
BtsCI GGATG 3 cut(s) 562, 580, 606
BtsI GCAGTG 1 cut(s) 423
BtsIMutI CAGTG 3 cut(s) 142, 165, 423
Cac8I GCNNGC 2 cut(s) 7, 330
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 1 cut(s) 1003
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 1 cut(s) 200
Csp6I GTAC 3 cut(s) 41, 767, 832
CviAII CATG 3 cut(s) 281, 544, 850
CviJI RGCY 9 cut(s) 9, 328, 366, 451, 592, 880, 917, 1036, 1067
CviKI_1 RGCY 9 cut(s) 9, 328, 366, 451, 592, 880, 917, 1036, 1067
CviQI GTAC 3 cut(s) 41, 767, 832
DdeI CTNAG 4 cut(s) 185, 324, 1004, 1046
DpnI GATC 4 cut(s) 125, 145, 158, 267
DpnII GATC 4 cut(s) 123, 143, 156, 265
DriI GACNNNNNGTC 1 cut(s) 790
Eam1104I CTCTTC 1 cut(s) 891
Eam1105I GACNNNNNGTC 1 cut(s) 790
EarI CTCTTC 1 cut(s) 891
EciI GGCGGA 1 cut(s) 309
Eco147I AGGCCT 1 cut(s) 1067
Eco31I GGTCTC 1 cut(s) 47
Eco47I GGWCC 1 cut(s) 27
Eco57I CTGAAG 1 cut(s) 915
EcoO109I RGGNCCY 1 cut(s) 27
EcoRI GAATTC 1 cut(s) 680
EcoRII CCWGG 1 cut(s) 343
FaeI CATG 3 cut(s) 284, 547, 853
FalI AAGNNNNNCTT 2 cut(s) 53, 85
FaqI GGGAC 1 cut(s) 40
FatI CATG 3 cut(s) 280, 543, 849
FauI CCCGC 1 cut(s) 548
FbaI TGATCA 1 cut(s) 156
Fnu4HI GCNGC 2 cut(s) 79, 1010
FokI GGATG 3 cut(s) 569, 587, 613
Fsp4HI GCNGC 2 cut(s) 79, 1010
FspBI CTAG 2 cut(s) 329, 1037
GlaI GCGC 1 cut(s) 1002
GluI GCNGC 2 cut(s) 79, 1010
GsuI CTGGAG 2 cut(s) 65, 927
HaeIII GGCC 1 cut(s) 1067
HgaI GACGC 1 cut(s) 200
HhaI GCGC 1 cut(s) 1003
Hin1II CATG 3 cut(s) 284, 547, 853
Hin6I GCGC 1 cut(s) 1001
HinP1I GCGC 1 cut(s) 1001
HindIII AAGCTT 1 cut(s) 590
HinfI GANTC 4 cut(s) 217, 529, 785, 977
Hpy166II GTNNAC 1 cut(s) 210
Hpy188I TCNGA 6 cut(s) 13, 143, 325, 534, 921, 945
Hpy188III TCNNGA 2 cut(s) 121, 677
Hpy8I GTNNAC 1 cut(s) 210
Hpy99I CGWCG 2 cut(s) 106, 194
HpyAV CCTTC 3 cut(s) 17, 137, 969
HpyCH4III ACNGT 3 cut(s) 274, 951, 973
HpyCH4IV ACGT 2 cut(s) 39, 104
HpyCH4V TGCA 8 cut(s) 34, 371, 428, 457, 488, 672, 715, 823
HpyF10VI GCNNNNNNNGC 3 cut(s) 886, 923, 1009
HpyF3I CTNAG 4 cut(s) 185, 324, 1004, 1046
HpySE526I ACGT 2 cut(s) 39, 104
Hsp92II CATG 3 cut(s) 284, 547, 853
HspAI GCGC 1 cut(s) 1001
KflI GGGWCCC 1 cut(s) 27
Ksp22I TGATCA 1 cut(s) 156
Kzo9I GATC 4 cut(s) 123, 143, 156, 265
LmnI GCTCC 2 cut(s) 46, 885
Lsp1109I GCAGC 1 cut(s) 1021
LweI GCATC 1 cut(s) 565
MaeI CTAG 2 cut(s) 329, 1037
MaeII ACGT 2 cut(s) 39, 104
MaeIII GTNAC 1 cut(s) 901
MalI GATC 4 cut(s) 125, 145, 158, 267
MboI GATC 4 cut(s) 123, 143, 156, 265
MboII GAAGA 5 cut(s) 61, 296, 791, 794, 908
MflI RGATCY 2 cut(s) 143, 265
MluCI AATT 9 cut(s) 244, 383, 411, 512, 563, 632, 680, 728, 797
MlyI GAGTC 3 cut(s) 211, 538, 779
MnlI CCTC 8 cut(s) 68, 128, 157, 349, 565, 589, 695, 892
MseI TTAA 5 cut(s) 231, 380, 624, 759, 867
MspR9I CCNGG 1 cut(s) 345
Mva1269I GAATGC 3 cut(s) 672, 819, 825
MvaI CCWGG 1 cut(s) 345
MwoI GCNNNNNNNGC 3 cut(s) 886, 923, 1009
NdeII GATC 4 cut(s) 123, 143, 156, 265
NheI GCTAGC 1 cut(s) 328
NlaIII CATG 3 cut(s) 284, 547, 853
NlaIV GGNNCC 3 cut(s) 28, 29, 239
NspI RCATGY 2 cut(s) 547, 853
PceI AGGCCT 1 cut(s) 1067
PciI ACATGT 1 cut(s) 849
PcsI WCGNNNNNNNCGW 1 cut(s) 977
PctI GAATGC 3 cut(s) 672, 819, 825
PfeI GAWTC 1 cut(s) 977
PkrI GCNGC 2 cut(s) 80, 1011
PleI GAGTC 3 cut(s) 211, 537, 779
PpsI GAGTC 3 cut(s) 211, 537, 779
PpuMI RGGWCCY 1 cut(s) 27
PscI ACATGT 1 cut(s) 849
Psp5II RGGWCCY 1 cut(s) 27
Psp6I CCWGG 1 cut(s) 343
PspGI CCWGG 1 cut(s) 343
PspN4I GGNNCC 3 cut(s) 28, 29, 239
PspPI GGNCC 1 cut(s) 27
PspPPI RGGWCCY 1 cut(s) 27
PstI CTGCAG 1 cut(s) 36
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 2 cut(s) 143, 265
RsaI GTAC 3 cut(s) 42, 768, 833
RsaNI GTAC 3 cut(s) 41, 767, 832
SaqAI TTAA 5 cut(s) 231, 380, 624, 759, 867
SatI GCNGC 2 cut(s) 79, 1010
Sau3AI GATC 4 cut(s) 123, 143, 156, 265
Sau96I GGNCC 1 cut(s) 27
ScaI AGTACT 1 cut(s) 768
SchI GAGTC 3 cut(s) 211, 538, 779
ScrFI CCNGG 1 cut(s) 345
SfaNI GCATC 1 cut(s) 565
SfcI CTRYAG 1 cut(s) 32
SinI GGWCC 1 cut(s) 27
SmlI CTYRAG 2 cut(s) 59, 593
SmoI CTYRAG 2 cut(s) 59, 593
Sse9I AATT 9 cut(s) 244, 383, 411, 512, 563, 632, 680, 728, 797
SseBI AGGCCT 1 cut(s) 1067
SsiI CCGC 4 cut(s) 78, 294, 519, 541
SspI AATATT 1 cut(s) 751
SspMI CTAG 2 cut(s) 329, 1037
StuI AGGCCT 1 cut(s) 1067
StyD4I CCNGG 1 cut(s) 343
TaaI ACNGT 3 cut(s) 274, 951, 973
TaiI ACGT 2 cut(s) 42, 107
TaqI TCGA 1 cut(s) 122
TasI AATT 9 cut(s) 244, 383, 411, 512, 563, 632, 680, 728, 797
TatI WGTACW 1 cut(s) 766
TauI GCSGC 1 cut(s) 81
TfiI GAWTC 1 cut(s) 977
Tru1I TTAA 5 cut(s) 231, 380, 624, 759, 867
Tru9I TTAA 5 cut(s) 231, 380, 624, 759, 867
TscAI CASTG 3 cut(s) 142, 172, 430
TseI GCWGC 1 cut(s) 1009
TspDTI ATGAA 5 cut(s) 297, 576, 617, 711, 792
TspGWI ACGGA 1 cut(s) 1040
TspRI CASTG 3 cut(s) 142, 172, 430
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 4 cut(s) 512, 563, 680, 728
XceI RCATGY 2 cut(s) 547, 853
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XspI CTAG 2 cut(s) 329, 1037
ZrmI AGTACT 1 cut(s) 768
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.