Rorug01G0223300

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001216
Physical Location & Seq
Forward (+)
32100311 .. 32104699
4389 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0223300.1

Sequence Viewer

Length: 753 bp
ATGCTGATGGTTCCCTCGTCTCTCTCTTATAAACCCACACCTCCCACACCAAGCGTTCATCAAAGTCACAACAAACAAATAATCCAATGCTGTTCAAGTCAGATCTCAAGACATCACAGGGCAGAGAGTGGTGGCATATCACATTGTGAAGAGAATTTGAATCGGAGGCTTATTGTGTTTCTTTCAGTCACGACAAGCTTGTTTCCAAATTTATCTTCTTCTGCGAAAACAAAGAGTAAAAGTCCATTTGATGAAAGACGCTTGCTGGAGCAAAACAGGCGTATACAGAAAGAAAATAATGCACCTGAAGAGTTTCCAAGTTTCATTAGAGAAGGTTTTCAGGTTAAGGTAGTAACGCCGGAAGACTACACAACACGTGAATCAGGACTTATATATCGGGATTTTGCAGTTGGTGAGGGTGATTGCCCACAGGCTGGTCAGCAGGTGATGTTCCACTATGTTGGCTACAATGAGTCAGGCCGTCGCATAGACAGCAGCTACAATCAAGGTTCTCCTGCCAGAATACGCATGGGAACAAATGCATTGGTCCCAGGATTTGAAGAAGGAATTCGTGACATGAAACCTGGGGGAAAAAGAAGAATAATCATTCCACCAGAACTTGGACCACCGGTAGGACCTTCCACCTTTTTCAGCTCAAAACAGTTTGAAGTTTTTGATGTGGAACTGCTAAGCATTCAAAACTGTGTAAGGAGGACTATAGCTGCTTTTTACTCTGATTTTGTCTGCGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ArsA_ATPase

Protein Analysis

250

Amino Acids

28.15

Weight (kDa)

8.52

Isoelectric Point (pI)

61.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FKBP_C PF00254 143 - 208 1.9e-17 FKBP-type peptidyl-prolyl cis-trans isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 30
AarI CACCTGC 1 cut(s) 433
Acc36I ACCTGC 1 cut(s) 433
AccB7I CCANNNNNTGG 2 cut(s) 434, 620
AccI GTMKAC 1 cut(s) 283
AcsI RAATTY 3 cut(s) 154, 208, 567
AcuI CTGAAG 1 cut(s) 327
AcvI CACGTG 1 cut(s) 377
AdeI CACNNNGTG 1 cut(s) 146
AfiI CCNNNNNNNGG 3 cut(s) 434, 620, 632
AflIII ACRYGT 1 cut(s) 374
AgeI ACCGGT 1 cut(s) 628
AgsI TTSAA 5 cut(s) 96, 160, 560, 668, 698
AjnI CCWGG 2 cut(s) 550, 583
AjuI GAANNNNNNNTTGG 2 cut(s) 199, 231
AluBI AGCT 4 cut(s) 198, 498, 654, 722
AluI AGCT 4 cut(s) 198, 498, 654, 722
Alw26I GTCTC 1 cut(s) 24
AoxI GGCC 1 cut(s) 478
ApeKI GCWGC 2 cut(s) 495, 722
ApoI RAATTY 3 cut(s) 154, 208, 567
AsiGI ACCGGT 1 cut(s) 628
Asp700I GAANNNNTTC 3 cut(s) 312, 336, 567
AspS9I GGNCC 3 cut(s) 547, 623, 635
AsuHPI GGTGA 3 cut(s) 425, 431, 457
AvaII GGWCC 3 cut(s) 547, 623, 635
BbrPI CACGTG 1 cut(s) 377
BbsI GAAGAC 1 cut(s) 369
BbvI GCAGC 2 cut(s) 507, 709
BceAI ACGGC 1 cut(s) 465
BciT130I CCWGG 2 cut(s) 552, 585
BcoDI GTCTC 1 cut(s) 24
BfmI CTRYAG 1 cut(s) 717
BfuAI ACCTGC 1 cut(s) 433
BglII AGATCT 1 cut(s) 102
BisI GCNGC 2 cut(s) 496, 723
BlpI GCTNAGC 1 cut(s) 689
BlsI GCNGC 2 cut(s) 497, 724
Bme1390I CCNGG 2 cut(s) 552, 585
Bme18I GGWCC 3 cut(s) 547, 623, 635
BmgT120I GGNCC 3 cut(s) 547, 623, 635
BmiI GGNNCC 2 cut(s) 12, 549
BmrFI CCNGG 2 cut(s) 552, 585
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 287
Bpu1102I GCTNAGC 1 cut(s) 689
BpuEI CTTGAG 1 cut(s) 91
BsaAI YACGTR 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 550, 584
BsaWI WCCGGW 1 cut(s) 628
Bsc4I CCNNNNNNNGG 3 cut(s) 434, 620, 632
Bse118I RCCGGY 1 cut(s) 628
BseBI CCWGG 2 cut(s) 552, 585
BseDI CCNNGG 2 cut(s) 550, 584
BseLI CCNNNNNNNGG 3 cut(s) 434, 620, 632
BseXI GCAGC 2 cut(s) 507, 709
BshFI GGCC 1 cut(s) 480
BshTI ACCGGT 1 cut(s) 628
BsiSI CCGG 2 cut(s) 359, 629
BslFI GGGAC 1 cut(s) 533
BslI CCNNNNNNNGG 3 cut(s) 434, 620, 632
BsmAI GTCTC 1 cut(s) 24
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 1 cut(s) 533
BsmI GAATGC 1 cut(s) 693
BsnI GGCC 1 cut(s) 480
Bsp143I GATC 1 cut(s) 102
Bsp1720I GCTNAGC 1 cut(s) 689
BspANI GGCC 1 cut(s) 480
BspLI GGNNCC 2 cut(s) 12, 549
BspMI ACCTGC 1 cut(s) 433
BsrFI RCCGGY 1 cut(s) 628
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 2 cut(s) 550, 584
BssMI GATC 1 cut(s) 102
BssNAI GTATAC 1 cut(s) 284
Bst1107I GTATAC 1 cut(s) 284
Bst2UI CCWGG 2 cut(s) 552, 585
Bst4CI ACNGT 2 cut(s) 663, 704
Bst6I CTCTTC 2 cut(s) 144, 303
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 1 cut(s) 689
BstKTI GATC 1 cut(s) 105
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 277, 492
BstNI CCWGG 2 cut(s) 552, 585
BstSCI CCNGG 2 cut(s) 550, 583
BstSFI CTRYAG 1 cut(s) 717
BstV1I GCAGC 2 cut(s) 507, 709
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 102
BstXI CCANNNNNNTGG 1 cut(s) 461
BstYI RGATCY 1 cut(s) 102
BstZ17I GTATAC 1 cut(s) 284
BsuRI GGCC 1 cut(s) 480
BveI ACCTGC 1 cut(s) 433
Cac8I GCNNGC 1 cut(s) 263
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 3 cut(s) 547, 623, 635
CseI GACGC 1 cut(s) 267
CspAI ACCGGT 1 cut(s) 628
CviAII CATG 2 cut(s) 529, 577
CviJI RGCY 8 cut(s) 169, 198, 434, 465, 480, 498, 654, 722
CviKI_1 RGCY 8 cut(s) 169, 198, 434, 465, 480, 498, 654, 722
DdeI CTNAG 1 cut(s) 689
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
DraIII CACNNNGTG 1 cut(s) 146
Eam1104I CTCTTC 2 cut(s) 144, 303
EarI CTCTTC 2 cut(s) 144, 303
Eco47I GGWCC 3 cut(s) 547, 623, 635
Eco57I CTGAAG 1 cut(s) 327
Eco72I CACGTG 1 cut(s) 377
EcoO109I RGGNCCY 1 cut(s) 635
EcoRI GAATTC 1 cut(s) 567
EcoRII CCWGG 2 cut(s) 550, 583
EcoT22I ATGCAT 1 cut(s) 544
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 2 cut(s) 532, 580
FaqI GGGAC 1 cut(s) 533
FatI CATG 2 cut(s) 528, 576
FblI GTMKAC 1 cut(s) 283
Fnu4HI GCNGC 2 cut(s) 496, 723
Fsp4HI GCNGC 2 cut(s) 496, 723
GluI GCNGC 2 cut(s) 496, 723
GsuI CTGGAG 1 cut(s) 287
HaeIII GGCC 1 cut(s) 480
HapII CCGG 2 cut(s) 359, 629
HgaI GACGC 1 cut(s) 267
Hin1II CATG 2 cut(s) 532, 580
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 3 cut(s) 160, 380, 473
HpaII CCGG 2 cut(s) 359, 629
HphI GGTGA 3 cut(s) 425, 431, 457
Hpy166II GTNNAC 1 cut(s) 284
Hpy188I TCNGA 3 cut(s) 102, 165, 736
Hpy188III TCNNGA 5 cut(s) 108, 190, 384, 398, 572
Hpy8I GTNNAC 1 cut(s) 284
Hpy99I CGWCG 1 cut(s) 486
HpyAV CCTTC 3 cut(s) 326, 557, 648
HpyCH4III ACNGT 2 cut(s) 663, 704
HpyCH4IV ACGT 1 cut(s) 376
HpyCH4V TGCA 3 cut(s) 302, 407, 542
HpyF10VI GCNNNNNNNGC 2 cut(s) 277, 492
HpyF3I CTNAG 1 cut(s) 689
HpySE526I ACGT 1 cut(s) 376
Hsp92II CATG 2 cut(s) 532, 580
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 268
Lsp1109I GCAGC 2 cut(s) 507, 709
MaeII ACGT 1 cut(s) 376
MaeIII GTNAC 4 cut(s) 65, 187, 352, 572
MalI GATC 1 cut(s) 104
MboI GATC 1 cut(s) 102
MboII GAAGA 7 cut(s) 161, 207, 210, 320, 374, 572, 609
MflI RGATCY 1 cut(s) 102
MluCI AATT 3 cut(s) 154, 208, 567
MlyI GAGTC 1 cut(s) 482
MnlI CCTC 5 cut(s) 25, 51, 159, 409, 705
Mph1103I ATGCAT 1 cut(s) 544
MroXI GAANNNNTTC 3 cut(s) 312, 336, 567
MseI TTAA 1 cut(s) 345
MspI CCGG 2 cut(s) 359, 629
MspR9I CCNGG 2 cut(s) 552, 585
Mva1269I GAATGC 1 cut(s) 693
MvaI CCWGG 2 cut(s) 552, 585
MwoI GCNNNNNNNGC 2 cut(s) 277, 492
NdeII GATC 1 cut(s) 102
NlaIII CATG 2 cut(s) 532, 580
NlaIV GGNNCC 2 cut(s) 12, 549
NmuCI GTSAC 3 cut(s) 65, 187, 572
NsiI ATGCAT 1 cut(s) 544
PaqCI CACCTGC 1 cut(s) 433
PctI GAATGC 1 cut(s) 693
PdmI GAANNNNTTC 3 cut(s) 312, 336, 567
PfeI GAWTC 2 cut(s) 160, 380
PflMI CCANNNNNTGG 2 cut(s) 434, 620
PinAI ACCGGT 1 cut(s) 628
PkrI GCNGC 2 cut(s) 497, 724
PleI GAGTC 1 cut(s) 481
PmaCI CACGTG 1 cut(s) 377
PmlI CACGTG 1 cut(s) 377
PpsI GAGTC 1 cut(s) 481
Ppu21I YACGTR 1 cut(s) 377
PpuMI RGGWCCY 1 cut(s) 635
PsiI TTATAA 1 cut(s) 30
Psp5II RGGWCCY 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 550, 583
PspCI CACGTG 1 cut(s) 377
PspGI CCWGG 2 cut(s) 550, 583
PspN4I GGNNCC 2 cut(s) 12, 549
PspPI GGNCC 3 cut(s) 547, 623, 635
PspPPI RGGWCCY 1 cut(s) 635
PsuI RGATCY 1 cut(s) 102
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 2 cut(s) 496, 723
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 3 cut(s) 547, 623, 635
SchI GAGTC 1 cut(s) 482
ScrFI CCNGG 2 cut(s) 552, 585
SfcI CTRYAG 1 cut(s) 717
SinI GGWCC 3 cut(s) 547, 623, 635
SmlI CTYRAG 1 cut(s) 106
SmoI CTYRAG 1 cut(s) 106
Sse9I AATT 3 cut(s) 154, 208, 567
StyD4I CCNGG 2 cut(s) 550, 583
TaaI ACNGT 2 cut(s) 663, 704
TaiI ACGT 1 cut(s) 379
TasI AATT 3 cut(s) 154, 208, 567
TfiI GAWTC 2 cut(s) 160, 380
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TseFI GTSAC 3 cut(s) 65, 187, 572
TseI GCWGC 2 cut(s) 495, 722
Tsp45I GTSAC 3 cut(s) 65, 187, 572
TspDTI ATGAA 4 cut(s) 47, 267, 313, 593
Van91I CCANNNNNTGG 2 cut(s) 434, 620
VpaK11BI GGWCC 3 cut(s) 547, 623, 635
XapI RAATTY 3 cut(s) 154, 208, 567
XcmI CCANNNNNNNNNTGG 1 cut(s) 526
XmiI GTMKAC 1 cut(s) 283
XmnI GAANNNNTTC 3 cut(s) 312, 336, 567
Zsp2I ATGCAT 1 cut(s) 544
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.