Rroxscaffold_4G00303360

SPX domain-containing membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001216
Physical Location & Seq
Forward (+)
23799334 .. 23805723
6390 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303360.1

Sequence Viewer

Length: 2160 bp
ATGGTTGCATTTGGGAAAAAGTTGAGAGAATCCCAGATTCAAGAATGGCAAGAATACTATATTGACTATAAGTTGTTGAAGAAAAAACTAAATCGGTATACACAACAAGTTGAAGTTGGAACAGAGAATCACCTCATTGTTCTGAAGGACTTCTCTAGATTGTTAGATTGTCAGATTGAAAAGTTTGTCATGTTTCTGCTGGAACAACAAGGAATTCTTGCCAGCAGGTTATCCAATCTCAGAGAACAGTATGATTCTCTTTTACAGCAGGCAGATGGAGAAAAAGTATGTGAACTACGAGAAGCATATCGATCAGTTGGTCAAGATCTCTTAAGAATTCTCTTTTTTGTTGAGATGAACGCCATTGGTTTGCGTAAGATATTGAAGAAGTTTGACAAGCGATTTGGGTTTAAATTTACCAATTACTATGTCAAAACCCGTGCAAATCATCCTTATTCCCAGTTACGACAAGTCTGTAAGCATGTGGGCATTGGAGCCGTTGTGGGAGCCATATCTCGAAACCTCGCGGACCTTCAAGATCATCGTGGGAGCTACATATCAATATATGATCAGCCTGCTTTATCCCACCCGGATCCTGTTGTTGATTCTATTAGAACAGCAGTGGACAGGTTGTCAAACTCGACGAGTTTCCTCCATTATTTGGGAAAACATGCGCTTATTATGCAGGAGGAGTTTCCAATTTCATCTGAGGATCCTACTGATGAAAGCACATATCATTTCATGTCACTTCTCTTAAATTTGGCAAATACATTTCTCTATATGGTCAATACATATATAATTGTCCCAACAGCTGACAGATACACCATGACCCTTGGAGCCGCAGCAACGGTCTGTGGTGTTGTGATTGGGTCAATGGCAGTTGCCCAGGTGTTTTCTTCAGTTTATTTCAGTGCATGGTCAAATAGATCATACATGAGACCACTTGTATTCAGTAGCATTGTTCTTCTTGTGGGAAATACCCTATATGCACTGGCGTATGATCTTGATTCATTATCAGTACTTCTGATTGGTCGACTCTTCTGTGGGTTAGGTTCTGCAAGAGCGGTTAACCGGCGTTATATCAGTGATTGTGTACCCCTTAAATTGCGAATGCAAGCTTCTGCTGGTTTTGTGAGTGCTAGTGCACTCGGAATGGCATGTGGTCCTGCTCTTGCTTGTTTATTCCAAACTGATTTCAAGATTTACAAGTTGACATTCAACGAAGCCACATTACCTGGTTGGGCCATGGCTATTGCATGGCTTGTATATTTGCTGTGGTTGTGGATTTCTTTTAGAGAGCCTTCTCGTGAAACTAAAGAAAATGTTGTGCAAGACGAATCTAATTCTGGAAGGTATATAGCTAGTTCTCCTGAAACTGAAGAAAATGCCTTGCCAGAGGAATCTAATTCTGGGAGGTTTATAAATATTTCCGTGGACCATAATTCTACACAACCATTACTAGTGAACTCCAACGCTAAGCAACAAGATGAAGATGGAGACGAAGATTGTGATGATGCTGAAGAAGATTCCAAAGAACTCCAGAGGCCAGTCAATTCAATTGTGTCAGCATATAGATTACTTACACCATCAGTAAAGGTTCAACTACTTATATATTTTATGCTCAAATATGCTATGGAGATTGTACTTGCTGAGTCGAGTCTCATCACCGGATATTATTTTATTTGGTCAACTAGCAGCGTGGCAATCTTTCTTGCATGTCTTGGGCTAACAGTGCTTCCAGTCAATGTTATTGTTGGGAGCTATATCAGCAACATATTTGAAGAAAGGCAGGTTCTATTGGCCTCTGAAGTTCTGGTGTGCATAGGTATACTACTGAGCTTCCACATATTGAACTCTTACTCTCTGCCTCAATATGTCGGCTCAGCCCTTCTAACTTTTGTGTCAGCTGAAGTCCTTGAAGGTGTGAACTTATCACTCCTATCTCGCGTTATGTCATCAAGGCTTTCTCGTGGGACTTTCAATGGCGGATTGCTTTCAACTGAGGCGGGGACCCTGGCCCGGGTAGTTGCAGATGGAACAATAACCCTAGCTGGGTATTTAGGTGTGAGCAAGGTCTTGAATACCACTTTGGTTCCTTCTCTGTTCTTCTGTGTCTGTTCCATTGTTGCCACCTGCTTCACCTACAACTCTCTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 SPX Sugar_tr

Protein Analysis

719

Amino Acids

80.34

Weight (kDa)

5.81

Isoelectric Point (pI)

41.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPX PF03105 4 - 36 2.3e-07 SPX domain
MFS_1 PF07690 256 - 651 4.8e-19 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1421
AarI CACCTGC 1 cut(s) 2141
Acc36I ACCTGC 3 cut(s) 216, 1780, 2141
AccB7I CCANNNNNTGG 1 cut(s) 661
AccBSI CCGCTC 1 cut(s) 1064
AccI GTMKAC 3 cut(s) 98, 1033, 1828
AccII CGCG 2 cut(s) 527, 1947
AciI CCGC 5 cut(s) 527, 840, 1064, 1986, 2006
AclWI GGATC 4 cut(s) 587, 600, 707, 720
AcsI RAATTY 4 cut(s) 213, 336, 413, 757
AcuI CTGAAG 6 cut(s) 164, 882, 1398, 1539, 1827, 1929
AfaI GTAC 3 cut(s) 1020, 1095, 1644
AfiI CCNNNNNNNGG 4 cut(s) 661, 2019, 2020, 2052
AflII CTTAAG 1 cut(s) 331
AhdI GACNNNNNGTC 1 cut(s) 631
AhlI ACTAGT 1 cut(s) 1459
AjnI CCWGG 3 cut(s) 885, 1234, 2013
AjuI GAANNNNNNNTTGG 2 cut(s) 2072, 2104
AluBI AGCT 8 cut(s) 552, 812, 1118, 1361, 1761, 1839, 1907, 2051
AluI AGCT 8 cut(s) 552, 812, 1118, 1361, 1761, 1839, 1907, 2051
Alw21I GWGCWC 1 cut(s) 1147
Alw26I GTCTC 3 cut(s) 931, 1491, 1664
Alw44I GTGCAC 1 cut(s) 1143
AlwI GGATC 4 cut(s) 587, 600, 707, 720
Ama87I CYCGRG 1 cut(s) 2019
AoxI GGCC 4 cut(s) 1242, 1544, 1800, 2016
ApaLI GTGCAC 1 cut(s) 1143
ApeKI GCWGC 2 cut(s) 842, 1695
ApoI RAATTY 4 cut(s) 213, 336, 413, 757
ArsI GACNNNNNNTTYG 2 cut(s) 386, 418
Asp700I GAANNNNTTC 1 cut(s) 149
AspLEI GCGC 1 cut(s) 676
AspS9I GGNCC 6 cut(s) 529, 1163, 1242, 1435, 2010, 2017
AsuC2I CCSGG 3 cut(s) 590, 2020, 2021
AsuHPI GGTGA 3 cut(s) 122, 1657, 2131
AvaI CYCGRG 1 cut(s) 2019
AvaII GGWCC 4 cut(s) 529, 1163, 1435, 2010
BaeGI GKGCMC 1 cut(s) 1147
BamHI GGATCC 2 cut(s) 592, 712
BarI GAAGNNNNNNTAC 2 cut(s) 1215, 1247
BauI CACGAG 2 cut(s) 1305, 1968
Bbv12I GWGCWC 1 cut(s) 1147
BbvI GCAGC 2 cut(s) 854, 1707
BccI CCATC 4 cut(s) 269, 1487, 1594, 2027
BceAI ACGGC 1 cut(s) 482
BciT130I CCWGG 3 cut(s) 887, 1236, 2015
BclI TGATCA 1 cut(s) 568
BcnI CCSGG 3 cut(s) 590, 2020, 2021
BcoDI GTCTC 3 cut(s) 931, 1491, 1664
BcuI ACTAGT 1 cut(s) 1459
BfaI CTAG 6 cut(s) 156, 1140, 1362, 1460, 1692, 2048
BfrI CTTAAG 1 cut(s) 331
BfuAI ACCTGC 3 cut(s) 216, 1780, 2141
BglII AGATCT 1 cut(s) 325
BisI GCNGC 3 cut(s) 840, 843, 1696
BlpI GCTNAGC 2 cut(s) 1476, 1882
BlsI GCNGC 3 cut(s) 841, 844, 1697
BmcAI AGTACT 1 cut(s) 1020
Bme1390I CCNGG 6 cut(s) 590, 887, 1236, 2015, 2020, 2021
Bme18I GGWCC 4 cut(s) 529, 1163, 1435, 2010
BmeRI GACNNNNNGTC 1 cut(s) 631
BmeT110I CYCGRG 1 cut(s) 2019
BmgT120I GGNCC 6 cut(s) 529, 1163, 1242, 1435, 2010, 2017
BmiI GGNNCC 8 cut(s) 496, 508, 594, 714, 838, 2011, 2012, 2094
BmrFI CCNGG 6 cut(s) 590, 887, 1236, 2015, 2020, 2021
BmrI ACTGGG 1 cut(s) 454
BmsI GCATC 1 cut(s) 1504
BmuI ACTGGG 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 1523
Bpu1102I GCTNAGC 2 cut(s) 1476, 1882
BpuMI CCSGG 3 cut(s) 590, 2020, 2021
Bsa29I ATCGAT 1 cut(s) 310
BsaI GGTCTC 1 cut(s) 931
BsaJI CCNNGG 6 cut(s) 832, 885, 1245, 1431, 2013, 2019
BsaWI WCCGGW 1 cut(s) 1667
BsaXI ACNNNNNCTCC 4 cut(s) 270, 300, 486, 516
Bsc4I CCNNNNNNNGG 4 cut(s) 661, 2019, 2020, 2052
Bse118I RCCGGY 1 cut(s) 1071
Bse1I ACTGG 4 cut(s) 460, 996, 1547, 1739
BseBI CCWGG 3 cut(s) 887, 1236, 2015
BseCI ATCGAT 1 cut(s) 310
BseDI CCNNGG 6 cut(s) 832, 885, 1245, 1431, 2013, 2019
BseGI GGATG 1 cut(s) 448
BseLI CCNNNNNNNGG 4 cut(s) 661, 2019, 2020, 2052
BseMII CTCAG 6 cut(s) 253, 699, 1641, 1826, 1896, 1992
BseNI ACTGG 4 cut(s) 460, 996, 1547, 1739
BseRI GAGGAG 1 cut(s) 704
BseSI GKGCMC 1 cut(s) 1147
BseXI GCAGC 2 cut(s) 854, 1707
BseYI CCCAGC 1 cut(s) 2051
Bsh1236I CGCG 2 cut(s) 527, 1947
BshFI GGCC 4 cut(s) 1244, 1546, 1802, 2018
BshVI ATCGAT 1 cut(s) 310
BsiHKAI GWGCWC 1 cut(s) 1147
BsiHKCI CYCGRG 1 cut(s) 2019
BsiSI CCGG 4 cut(s) 590, 1072, 1668, 2020
BslFI GGGAC 3 cut(s) 788, 1987, 2023
BslI CCNNNNNNNGG 4 cut(s) 661, 2019, 2020, 2052
BsmAI GTCTC 3 cut(s) 931, 1491, 1664
BsmBI CGTCTC 1 cut(s) 1491
BsmFI GGGAC 3 cut(s) 788, 1987, 2023
BsmI GAATGC 1 cut(s) 1116
BsnI GGCC 4 cut(s) 1244, 1546, 1802, 2018
Bso31I GGTCTC 1 cut(s) 931
BsoBI CYCGRG 1 cut(s) 2019
Bsp1286I GDGCHC 1 cut(s) 1147
Bsp143I GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
Bsp1720I GCTNAGC 2 cut(s) 1476, 1882
Bsp19I CCATGG 1 cut(s) 1245
BspACI CCGC 5 cut(s) 527, 840, 1064, 1986, 2006
BspANI GGCC 4 cut(s) 1244, 1546, 1802, 2018
BspCNI CTCAG 6 cut(s) 252, 700, 1642, 1827, 1895, 1993
BspDI ATCGAT 1 cut(s) 310
BspFNI CGCG 2 cut(s) 527, 1947
BspLI GGNNCC 8 cut(s) 496, 508, 594, 714, 838, 2011, 2012, 2094
BspMI ACCTGC 3 cut(s) 216, 1780, 2141
BspPI GGATC 4 cut(s) 587, 600, 707, 720
BspTI CTTAAG 1 cut(s) 331
BspTNI GGTCTC 1 cut(s) 931
BsrBI CCGCTC 1 cut(s) 1064
BsrFI RCCGGY 1 cut(s) 1071
BsrI ACTGG 4 cut(s) 460, 996, 1547, 1739
BssAI RCCGGY 1 cut(s) 1071
BssECI CCNNGG 6 cut(s) 832, 885, 1245, 1431, 2013, 2019
BssMI GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
BssNAI GTATAC 2 cut(s) 99, 1829
BssSI CACGAG 2 cut(s) 1305, 1968
BssT1I CCWWGG 2 cut(s) 832, 1245
Bst1107I GTATAC 2 cut(s) 99, 1829
Bst2BI CACGAG 2 cut(s) 1305, 1968
Bst2UI CCWGG 3 cut(s) 887, 1236, 2015
Bst4CI ACNGT 3 cut(s) 249, 850, 1732
Bst6I CTCTTC 1 cut(s) 1043
BstAFI CTTAAG 1 cut(s) 331
BstC8I GCNNGC 4 cut(s) 223, 270, 576, 1116
BstDEI CTNAG 7 cut(s) 239, 708, 1476, 1650, 1835, 1882, 2001
BstDSI CCRYGG 2 cut(s) 1245, 1431
BstF5I GGATG 1 cut(s) 448
BstFNI CGCG 2 cut(s) 527, 1947
BstHHI GCGC 1 cut(s) 676
BstKTI GATC 8 cut(s) 314, 328, 541, 571, 595, 715, 929, 1003
BstMAI GTCTC 3 cut(s) 931, 1491, 1664
BstMBI GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
BstMWI GCNNNNNNNGC 3 cut(s) 682, 1732, 1767
BstNI CCWGG 3 cut(s) 887, 1236, 2015
BstNSI RCATGY 4 cut(s) 485, 674, 1161, 1719
BstSCI CCNGG 6 cut(s) 588, 885, 1234, 2013, 2018, 2019
BstSLI GKGCMC 1 cut(s) 1147
BstUI CGCG 2 cut(s) 527, 1947
BstV1I GCAGC 2 cut(s) 854, 1707
BstX2I RGATCY 3 cut(s) 325, 592, 712
BstYI RGATCY 3 cut(s) 325, 592, 712
BstZ17I GTATAC 2 cut(s) 99, 1829
Bsu15I ATCGAT 1 cut(s) 310
BsuRI GGCC 4 cut(s) 1244, 1546, 1802, 2018
BsuTUI ATCGAT 1 cut(s) 310
BtgI CCRYGG 2 cut(s) 1245, 1431
BtsCI GGATG 1 cut(s) 448
BtsI GCAGTG 1 cut(s) 627
BtsIMutI CAGTG 5 cut(s) 627, 916, 989, 1090, 1737
BveI ACCTGC 3 cut(s) 216, 1780, 2141
Cac8I GCNNGC 4 cut(s) 223, 270, 576, 1116
CfoI GCGC 1 cut(s) 676
Cfr10I RCCGGY 1 cut(s) 1071
Cfr13I GGNCC 6 cut(s) 529, 1163, 1242, 1435, 2010, 2017
Cfr9I CCCGGG 1 cut(s) 2019
ClaI ATCGAT 1 cut(s) 310
CsiI ACCWGGT 1 cut(s) 1234
Csp6I GTAC 3 cut(s) 1019, 1094, 1643
CviQI GTAC 3 cut(s) 1019, 1094, 1643
DdeI CTNAG 7 cut(s) 239, 708, 1476, 1650, 1835, 1882, 2001
DpnI GATC 8 cut(s) 313, 327, 540, 570, 594, 714, 928, 1002
DpnII GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
DraI TTTAAA 1 cut(s) 412
DriI GACNNNNNGTC 1 cut(s) 631
Eam1104I CTCTTC 1 cut(s) 1043
Eam1105I GACNNNNNGTC 1 cut(s) 631
EarI CTCTTC 1 cut(s) 1043
EciI GGCGGA 1 cut(s) 2001
Eco130I CCWWGG 2 cut(s) 832, 1245
Eco31I GGTCTC 1 cut(s) 931
Eco47I GGWCC 4 cut(s) 529, 1163, 1435, 2010
Eco57I CTGAAG 6 cut(s) 164, 882, 1398, 1539, 1827, 1929
Eco88I CYCGRG 1 cut(s) 2019
EcoO109I RGGNCCY 1 cut(s) 2010
EcoRI GAATTC 2 cut(s) 213, 336
EcoRII CCWGG 3 cut(s) 885, 1234, 2013
EcoT14I CCWWGG 2 cut(s) 832, 1245
ErhI CCWWGG 2 cut(s) 832, 1245
Esp3I CGTCTC 1 cut(s) 1491
FalI AAGNNNNNCTT 2 cut(s) 201, 233
FaqI GGGAC 3 cut(s) 788, 1987, 2023
FauI CCCGC 1 cut(s) 1999
FbaI TGATCA 1 cut(s) 568
FblI GTMKAC 3 cut(s) 98, 1033, 1828
Fnu4HI GCNGC 3 cut(s) 840, 843, 1696
FokI GGATG 1 cut(s) 435
Fsp4HI GCNGC 3 cut(s) 840, 843, 1696
FspBI CTAG 6 cut(s) 156, 1140, 1362, 1460, 1692, 2048
GlaI GCGC 1 cut(s) 675
GluI GCNGC 3 cut(s) 840, 843, 1696
GsaI CCCAGC 1 cut(s) 2055
GsuI CTGGAG 1 cut(s) 1523
HaeIII GGCC 4 cut(s) 1244, 1546, 1802, 2018
HapII CCGG 4 cut(s) 590, 1072, 1668, 2020
HhaI GCGC 1 cut(s) 676
Hin6I GCGC 1 cut(s) 674
HinP1I GCGC 1 cut(s) 674
HincII GTYRAC 4 cut(s) 1034, 1069, 1212, 1689
HindII GTYRAC 4 cut(s) 1034, 1069, 1212, 1689
HindIII AAGCTT 1 cut(s) 1116
HpaI GTTAAC 1 cut(s) 1069
HpaII CCGG 4 cut(s) 590, 1072, 1668, 2020
HphI GGTGA 3 cut(s) 122, 1657, 2131
Hpy188I TCNGA 7 cut(s) 144, 174, 242, 709, 1026, 1151, 1807
Hpy99I CGWCG 1 cut(s) 646
HpyAV CCTTC 7 cut(s) 139, 542, 1311, 1344, 1898, 1913, 2106
HpyCH4III ACNGT 3 cut(s) 249, 850, 1732
HpyF10VI GCNNNNNNNGC 3 cut(s) 682, 1732, 1767
HpyF3I CTNAG 7 cut(s) 239, 708, 1476, 1650, 1835, 1882, 2001
HspAI GCGC 1 cut(s) 674
KflI GGGWCCC 1 cut(s) 2010
Ksp22I TGATCA 1 cut(s) 568
KspAI GTTAAC 1 cut(s) 1069
Kzo9I GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
LmnI GCTCC 5 cut(s) 494, 506, 549, 836, 1758
Lsp1109I GCAGC 2 cut(s) 854, 1707
LweI GCATC 1 cut(s) 1504
MabI ACCWGGT 1 cut(s) 1234
MaeI CTAG 6 cut(s) 156, 1140, 1362, 1460, 1692, 2048
MaeIII GTNAC 2 cut(s) 462, 744
MalI GATC 8 cut(s) 313, 327, 540, 570, 594, 714, 928, 1002
MbiI CCGCTC 1 cut(s) 1064
MboI GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
MfeI CAATTG 1 cut(s) 1557
MflI RGATCY 3 cut(s) 325, 592, 712
MhlI GDGCHC 1 cut(s) 1147
MlyI GAGTC 3 cut(s) 1029, 1661, 1666
MmeI TCCRAC 2 cut(s) 97, 1494
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 5 cut(s) 332, 411, 755, 1068, 1101
MspA1I CMGCKG 2 cut(s) 812, 1907
MspCI CTTAAG 1 cut(s) 331
MspI CCGG 4 cut(s) 590, 1072, 1668, 2020
MspR9I CCNGG 6 cut(s) 590, 887, 1236, 2015, 2020, 2021
MunI CAATTG 1 cut(s) 1557
Mva1269I GAATGC 1 cut(s) 1116
MvaI CCWGG 3 cut(s) 887, 1236, 2015
MvnI CGCG 2 cut(s) 527, 1947
MwoI GCNNNNNNNGC 3 cut(s) 682, 1732, 1767
NciI CCSGG 3 cut(s) 590, 2020, 2021
NcoI CCATGG 1 cut(s) 1245
NdeII GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
NlaIV GGNNCC 8 cut(s) 496, 508, 594, 714, 838, 2011, 2012, 2094
NmuCI GTSAC 1 cut(s) 744
NspI RCATGY 4 cut(s) 485, 674, 1161, 1719
PaqCI CACCTGC 1 cut(s) 2141
PctI GAATGC 1 cut(s) 1116
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 9 cut(s) 29, 37, 127, 254, 605, 1007, 1337, 1400, 1526
PflMI CCANNNNNTGG 1 cut(s) 661
PkrI GCNGC 3 cut(s) 841, 844, 1697
PleI GAGTC 3 cut(s) 1029, 1660, 1665
PpsI GAGTC 3 cut(s) 1029, 1660, 1665
PpuMI RGGWCCY 1 cut(s) 2010
PsiI TTATAA 1 cut(s) 1421
Psp5II RGGWCCY 1 cut(s) 2010
Psp6I CCWGG 3 cut(s) 885, 1234, 2013
PspFI CCCAGC 1 cut(s) 2051
PspGI CCWGG 3 cut(s) 885, 1234, 2013
PspN4I GGNNCC 8 cut(s) 496, 508, 594, 714, 838, 2011, 2012, 2094
PspPI GGNCC 6 cut(s) 529, 1163, 1242, 1435, 2010, 2017
PspPPI RGGWCCY 1 cut(s) 2010
PsuI RGATCY 3 cut(s) 325, 592, 712
PvuII CAGCTG 2 cut(s) 812, 1907
RsaI GTAC 3 cut(s) 1020, 1095, 1644
RsaNI GTAC 3 cut(s) 1019, 1094, 1643
SalI GTCGAC 1 cut(s) 1032
SaqAI TTAA 5 cut(s) 332, 411, 755, 1068, 1101
SatI GCNGC 3 cut(s) 840, 843, 1696
Sau3AI GATC 8 cut(s) 311, 325, 538, 568, 592, 712, 926, 1000
Sau96I GGNCC 6 cut(s) 529, 1163, 1242, 1435, 2010, 2017
ScaI AGTACT 1 cut(s) 1020
SchI GAGTC 3 cut(s) 1029, 1661, 1666
ScrFI CCNGG 6 cut(s) 590, 887, 1236, 2015, 2020, 2021
SduI GDGCHC 1 cut(s) 1147
SexAI ACCWGGT 1 cut(s) 1234
SfaNI GCATC 1 cut(s) 1504
SinI GGWCC 4 cut(s) 529, 1163, 1435, 2010
SmaI CCCGGG 1 cut(s) 2021
SmlI CTYRAG 1 cut(s) 331
SmoI CTYRAG 1 cut(s) 331
SpeI ACTAGT 1 cut(s) 1459
SsiI CCGC 5 cut(s) 527, 840, 1064, 1986, 2006
SspI AATATT 1 cut(s) 1426
SspMI CTAG 6 cut(s) 156, 1140, 1362, 1460, 1692, 2048
StyD4I CCNGG 6 cut(s) 588, 885, 1234, 2013, 2018, 2019
StyI CCWWGG 2 cut(s) 832, 1245
TaaI ACNGT 3 cut(s) 249, 850, 1732
TaqI TCGA 5 cut(s) 310, 517, 641, 1033, 1655
TatI WGTACW 2 cut(s) 1018, 1642
TauI GCSGC 1 cut(s) 842
TfiI GAWTC 9 cut(s) 29, 37, 127, 254, 605, 1007, 1337, 1400, 1526
Tru1I TTAA 5 cut(s) 332, 411, 755, 1068, 1101
Tru9I TTAA 5 cut(s) 332, 411, 755, 1068, 1101
TscAI CASTG 5 cut(s) 627, 916, 996, 1090, 1737
TseFI GTSAC 1 cut(s) 744
TseI GCWGC 2 cut(s) 842, 1695
Tsp45I GTSAC 1 cut(s) 744
TspDTI ATGAA 6 cut(s) 371, 693, 730, 738, 999, 1503
TspGWI ACGGA 1 cut(s) 1420
TspMI CCCGGG 1 cut(s) 2019
TspRI CASTG 5 cut(s) 627, 916, 996, 1090, 1737
Van91I CCANNNNNTGG 1 cut(s) 661
Vha464I CTTAAG 1 cut(s) 331
VneI GTGCAC 1 cut(s) 1143
VpaK11BI GGWCC 4 cut(s) 529, 1163, 1435, 2010
XapI RAATTY 4 cut(s) 213, 336, 413, 757
XbaI TCTAGA 1 cut(s) 155
XceI RCATGY 4 cut(s) 485, 674, 1161, 1719
XmaI CCCGGG 1 cut(s) 2019
XmiI GTMKAC 3 cut(s) 98, 1033, 1828
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 6 cut(s) 156, 1140, 1362, 1460, 1692, 2048
ZrmI AGTACT 1 cut(s) 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.