Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001216

Physical Location & Seq

Genomic Coordinates Forward (+)

16904023 .. 16918731

Gene Structure 14709 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1069900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2013 bp

ATGAAGAAAAAAGTAAGACACTATTCTCAACAAATTAAAGTTGGAGCAATAGACCGGAGGCATGTCCTAAAGGACTTTTCAAGGATGCTGGATAATCAGATTGAGACTATTGTCCTTTTCCTGTTGGAACAACAAGGACTACTTGCAAGCAGGATAGCCAAACTTGGAGAACAACATGATGCTCTTCTGCAGCAACCTGATATATCCAAAATATTTGAACTACGAGAAGCTTATAGACAAGTGGGCCGTGATCTTCTGAAGCTTCTCTTTTTTGTTGAGGTGAATGCCATTGGTCTGCGTAAGATATTGAAGAAGTTCGATAAACGCTTTGGATATAGATTCACCGACTACTATGTCAAAACTCGTGCGAATCATCCTTACTCACAGCTCCAGCAAGTGTTTAAGCATGTGGGCATAGGGGCTGTTGTAGGAGCTGTATCTCGCAATCTTCATGAACTTCAGGACCGACAAGGAAGCTACTTATCAATATATGATCAGCCTGCTCTTCCCCTCCAGGATCCTGTTATTGATTCAGTAAAAGCAGCTGTGGACAGGTTAACCAATTCAACAAACTTTCTGAACTTTTTGGGGCAACATGCACTTATTCTGCAAGAAGATATGCTGCCTGAACAAGTTGATGATCAGAGATACCACTTTATGTCACTTGTCTTGAACTTAGTGAACACGTTCCTTTACATGGTCAATACATATATTATTGTTCCTACAGCAGATGACTACTCCATGAGCCTTGGTGCTGCAGCGACAGTTTGTGGTGTTGTGATTGGAGCAATGGCTGTTGCACAGGTGTTTTCTTCAGTGTATTTTAGTGCATGGTCAAATCGGTCATACTTCAAGCCTCTTGTATTTAGCAGTATTGTTCTTTTTGTGGGAAACACCCTGTATGCTCTGGCTTATGATCTTAATTCGATATGGGTACTGTTGATTGGTCGTCTTTTCTGTGGGCTGGGTTCTGCAAGAGCTGTTAACAGGCGTTATATTAGTGATTGTGTACCTCTGAAAATCCGCATGCAGGCGTCAGCAGCTTTTGTTAGTGCTAGTGCCCTTGGAATGGCATGTGGTCCTGCTCTTGCTGGCTTACTTCAAACTCATTTCAAGATTTACAAGCTTACATTCAATCAAGACACTTTGCCTGGTTGGGTTATGGCTATAGCCTGGCTTCTATACTTAATATGGCTGTGGATCTCATTTAGAGAACCTGCTCGTGAAGATGAAGTGAGTTCTGTACAAGAAGGATCCAATAGCAGAACAGCAGTAAACGATGCACTCGAGAAAGGTCTTAATCAACCATTGCTCATTAGTGCTGAAGACAAGAGAGAAGATGAAGATGGTGACCAAGATATTGATGAAAGTGAAGAAGCTCCTGAGGAATCTCGTCGACCAGCCACTTCAATTCGATCCGCATATCAACTACTTACACCCTCTGTAAAGGTTCAATTGTTGATATATTTCATGCTCAAGTATGCTATGGAGATTTTACTTTCAGAATCTAGTGTTGTTACGACATACTACTTTAGCTGGAATACTAGCAAAGTCGCAATTTTTCTGGCGTGTCTTGGATTAACCGTTCTTCCAGTAAATATTCTTGTTGGGAGCTACATTAGCAACATGTTTGAAGACAGGCTAATTTTATTAGTATCTGAAATAGTGGTTTGCATAGGCATAGTTATGAGCTTCCATATGTTCAATCCGTACTCTGTGATACAATACGTCAGCTCAGGGCTCATCATGTTTGTTTCGGCTGAAGTACTTGAAGGTGTCAACTTGTCACTTCTCTCGAGGGTTATGTCATCGAGGCTTTCCCGTGGAACATACAATGGTGGATTACTTTCAACAGAAGCTGGGACACTCGCTCGAGTAGTTGCAGATGCCACCATAACTCTGGCCGGATACTTGGGAGAAAGTAGGCTCTTGAACATCACCTTGCTTCCGTCCCTCGTGATTTGCGTTTCTTCCATTATTGCCACCTGCTACACATACAACTCTCTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0008150 GO:0009705 GO:0015698 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 SPX

Protein Analysis

671

Amino Acids

75.03

Weight (kDa)

6.58

Isoelectric Point (pI)

41.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	83 - 121	5.6e-07	SPX domain
MFS_1	PF07690	227 - 613	2.5e-25	Major Facilitator Superfamily
Sugar_tr	PF00083	232 - 391	1.3e-06	Sugar (and other) transporter

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001216)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT4G11810 AT4G11810 AT4G11810 AT4G22990 AT4G22990 AT4G22990
fragaria_vesca	FvH4_5g01390 FvH4_7g12920 FvH4_7g12920 FvH4_7g12920
malus_domestica	MD02G1195400.v1.1 MD04G1068900.v1.1 MD06G1069900.v1.1
prunus_persica	Prupe.2G153100_v2.0.a1 Prupe.2G153100_v2.0.a1 Prupe.2G153100_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1
pyrus_communis	pycom02g15930 pycom04g06270 pycom06g07090
rosa_chinensis	RchiOBHm_Chr1g0352001 RchiOBHm_Chr7g0199491
rosa_laevigata	RLG00000003789 RLG00000028368
rosa_multiflora	Rmu_sc0003549.1_g000001 Rmu_sc0004009.1_g000006 Rmu_sc0007728.1_g000012
rosa_roxburghii	Rroxscaffold_3G00256680 Rroxscaffold_4G00303360
rosa_rugosa	Rorug01G0223300 Rorug01G0223400 Rorug07G0054600 Rorug07G0054700 Rorug07G0054800 Rorug07G0054900
rosa_samantha	Rh1AG237400 Rh1BG207600 Rh1CG221800 Rh1DG234600 Rh7AG179600 Rh7BG182900 Rh7CG188200 Rh7DG182300
rosa_wichuraiana	Rw1G020650 Rw7G015770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1994
AasI	GACNNNNNNGTC	1 cut(s)	353
Acc36I	ACCTGC	2 cut(s)	1225, 1994
AccI	GTMKAC	1 cut(s)	1396
AciI	CCGC	2 cut(s)	1024, 1419
AclWI	GGATC	6 cut(s)	512, 525, 1208, 1248, 1261, 1410
AcoI	YGGCCR	1 cut(s)	1902
AcuI	CTGAAG	5 cut(s)	278, 443, 798, 1344, 1782
AcyI	GRCGYC	1 cut(s)	1034
AfaI	GTAC	5 cut(s)	936, 1011, 1245, 1712, 1767
AfiI	CCNNNNNNNGG	3 cut(s)	697, 1030, 1069
AflIII	ACRYGT	2 cut(s)	684, 1626
AjnI	CCWGG	3 cut(s)	513, 1150, 1172
AloI	GAACNNNNNNTCC	2 cut(s)	1569, 1601
Alw26I	GTCTC	1 cut(s)	98
AlwI	GGATC	6 cut(s)	512, 525, 1208, 1248, 1261, 1410
AlwNI	CAGNNNCTG	1 cut(s)	1859
Ama87I	CYCGRG	3 cut(s)	1286, 1795, 1872
AoxI	GGCC	2 cut(s)	244, 1902
ApeKI	GCWGC	6 cut(s)	190, 542, 622, 755, 758, 1040
Asp700I	GAANNNNTTC	2 cut(s)	314, 686
AspS9I	GGNCC	3 cut(s)	244, 463, 1079
AsuHPI	GGTGA	4 cut(s)	292, 334, 1361, 1930
AvaI	CYCGRG	3 cut(s)	1286, 1795, 1872
AvaII	GGWCC	2 cut(s)	463, 1079
AxyI	CCTNAGG	1 cut(s)	1383
BaeGI	GKGCMC	1 cut(s)	1063
BamHI	GGATCC	2 cut(s)	517, 1253
BanII	GRGCYC	1 cut(s)	1743
BauI	CACGAG	3 cut(s)	363, 1221, 1955
BbsI	GAAGAC	2 cut(s)	1332, 1641
BbvI	GCAGC	6 cut(s)	202, 554, 609, 742, 770, 1052
BccI	CCATC	1 cut(s)	1340
BceAI	ACGGC	1 cut(s)	231
BciT130I	CCWGG	3 cut(s)	515, 1152, 1174
BciVI	GTATCC	1 cut(s)	1901
BclI	TGATCA	2 cut(s)	493, 640
BcoDI	GTCTC	1 cut(s)	98
BfaI	CTAG	3 cut(s)	1056, 1509, 1545
BfmI	CTRYAG	4 cut(s)	188, 723, 756, 1167
BfuAI	ACCTGC	2 cut(s)	1225, 1994
BfuI	GTATCC	1 cut(s)	1901
BisI	GCNGC	6 cut(s)	191, 543, 623, 756, 759, 1041
BlsI	GCNGC	6 cut(s)	192, 544, 624, 757, 760, 1042
BmcAI	AGTACT	1 cut(s)	1767
Bme1390I	CCNGG	3 cut(s)	515, 1152, 1174
Bme18I	GGWCC	2 cut(s)	463, 1079
BmeT110I	CYCGRG	3 cut(s)	1286, 1795, 1872
BmgT120I	GGNCC	3 cut(s)	244, 463, 1079
BmiI	GGNNCC	2 cut(s)	519, 1255
BmrFI	CCNGG	3 cut(s)	515, 1152, 1174
BmsI	GCATC	4 cut(s)	75, 169, 1270, 1876
BoxI	GACNNNNGTC	1 cut(s)	110
BpiI	GAAGAC	2 cut(s)	1332, 1641
BpmI	CTGGAG	2 cut(s)	374, 497
Bpu10I	CCTNAGC	1 cut(s)	1735
BpuEI	CTTGAG	1 cut(s)	1460
BsaHI	GRCGYC	1 cut(s)	1034
BsaJI	CCNNGG	3 cut(s)	748, 1063, 1822
BsaWI	WCCGGW	1 cut(s)	54
Bsc4I	CCNNNNNNNGG	3 cut(s)	697, 1030, 1069
Bse1I	ACTGG	1 cut(s)	1592
Bse21I	CCTNAGG	1 cut(s)	1383
Bse3DI	GCAATG	2 cut(s)	795, 1307
BseBI	CCWGG	3 cut(s)	515, 1152, 1174
BseDI	CCNNGG	3 cut(s)	748, 1063, 1822
BseGI	GGATG	2 cut(s)	90, 373
BseLI	CCNNNNNNNGG	3 cut(s)	697, 1030, 1069
BseMI	GCAATG	2 cut(s)	795, 1307
BseMII	CTCAG	2 cut(s)	1374, 1749
BseNI	ACTGG	1 cut(s)	1592
BseSI	GKGCMC	1 cut(s)	1063
BseXI	GCAGC	6 cut(s)	202, 554, 609, 742, 770, 1052
BseYI	CCCAGC	2 cut(s)	964, 1859
BshFI	GGCC	2 cut(s)	246, 1904
BsiHKCI	CYCGRG	3 cut(s)	1286, 1795, 1872
BsiSI	CCGG	2 cut(s)	55, 1905
BslFI	GGGAC	2 cut(s)	1876, 1936
BslI	CCNNNNNNNGG	3 cut(s)	697, 1030, 1069
BsmAI	GTCTC	1 cut(s)	98
BsmFI	GGGAC	2 cut(s)	1876, 1936
BsmI	GAATGC	1 cut(s)	289
BsnI	GGCC	2 cut(s)	246, 1904
BsoBI	CYCGRG	3 cut(s)	1286, 1795, 1872
Bsp1286I	GDGCHC	2 cut(s)	1063, 1743
Bsp1407I	TGTACA	1 cut(s)	1243
Bsp143I	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
BspACI	CCGC	2 cut(s)	1024, 1419
BspANI	GGCC	2 cut(s)	246, 1904
BspCNI	CTCAG	2 cut(s)	1375, 1748
BspHI	TCATGA	1 cut(s)	451
BspLI	GGNNCC	2 cut(s)	519, 1255
BspMAI	CTGCAG	2 cut(s)	192, 760
BspMI	ACCTGC	2 cut(s)	1225, 1994
BspPI	GGATC	6 cut(s)	512, 525, 1208, 1248, 1261, 1410
BspQI	GCTCTTC	2 cut(s)	189, 510
BsrDI	GCAATG	2 cut(s)	795, 1307
BsrGI	TGTACA	1 cut(s)	1243
BsrI	ACTGG	1 cut(s)	1592
BssECI	CCNNGG	3 cut(s)	748, 1063, 1822
BssMI	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
BssNI	GRCGYC	1 cut(s)	1034
BssSI	CACGAG	3 cut(s)	363, 1221, 1955
BssT1I	CCWWGG	2 cut(s)	748, 1063
Bst2BI	CACGAG	3 cut(s)	363, 1221, 1955
Bst2UI	CCWGG	3 cut(s)	515, 1152, 1174
Bst4CI	ACNGT	3 cut(s)	766, 939, 1585
Bst6I	CTCTTC	2 cut(s)	189, 510
BstACI	GRCGYC	1 cut(s)	1034
BstAUI	TGTACA	1 cut(s)	1243
BstC8I	GCNNGC	5 cut(s)	148, 501, 1028, 1032, 1093
BstDEI	CTNAG	3 cut(s)	676, 1383, 1735
BstDSI	CCRYGG	1 cut(s)	1822
BstEII	GGTNACC	1 cut(s)	1349
BstF5I	GGATG	2 cut(s)	90, 373
BstKTI	GATC	8 cut(s)	253, 496, 520, 643, 919, 1203, 1256, 1418
BstMAI	GTCTC	1 cut(s)	98
BstMBI	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
BstMWI	GCNNNNNNNGC	2 cut(s)	1040, 1620
BstNI	CCWGG	3 cut(s)	515, 1152, 1174
BstNSI	RCATGY	6 cut(s)	65, 410, 599, 1030, 1077, 1630
BstPAI	GACNNNNGTC	1 cut(s)	110
BstPI	GGTNACC	1 cut(s)	1349
BstSCI	CCNGG	3 cut(s)	513, 1150, 1172
BstSFI	CTRYAG	4 cut(s)	188, 723, 756, 1167
BstSLI	GKGCMC	1 cut(s)	1063
BstV1I	GCAGC	6 cut(s)	202, 554, 609, 742, 770, 1052
BstV2I	GAAGAC	2 cut(s)	1332, 1641
BstX2I	RGATCY	3 cut(s)	517, 1200, 1253
BstXI	CCANNNNNNTGG	1 cut(s)	1900
BstYI	RGATCY	3 cut(s)	517, 1200, 1253
Bsu36I	CCTNAGG	1 cut(s)	1383
BsuI	GTATCC	1 cut(s)	1901
BsuRI	GGCC	2 cut(s)	246, 1904
BtgI	CCRYGG	1 cut(s)	1822
BtsCI	GGATG	2 cut(s)	90, 373
BtsIMutI	CAGTG	1 cut(s)	822
BveI	ACCTGC	2 cut(s)	1225, 1994
Cac8I	GCNNGC	5 cut(s)	148, 501, 1028, 1032, 1093
CaiI	CAGNNNCTG	1 cut(s)	1859
CciI	TCATGA	1 cut(s)	451
Cfr13I	GGNCC	3 cut(s)	244, 463, 1079
CseI	GACGC	1 cut(s)	1023
Csp6I	GTAC	5 cut(s)	935, 1010, 1244, 1711, 1766
CviQI	GTAC	5 cut(s)	935, 1010, 1244, 1711, 1766
DdeI	CTNAG	3 cut(s)	676, 1383, 1735
DpnI	GATC	8 cut(s)	252, 495, 519, 642, 918, 1202, 1255, 1417
DpnII	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
DrdI	GACNNNNNNGTC	1 cut(s)	353
DseDI	GACNNNNNNGTC	1 cut(s)	353
EaeI	YGGCCR	1 cut(s)	1902
Eam1104I	CTCTTC	2 cut(s)	189, 510
EarI	CTCTTC	2 cut(s)	189, 510
Eco130I	CCWWGG	2 cut(s)	748, 1063
Eco24I	GRGCYC	1 cut(s)	1743
Eco47I	GGWCC	2 cut(s)	463, 1079
Eco57I	CTGAAG	5 cut(s)	278, 443, 798, 1344, 1782
Eco81I	CCTNAGG	1 cut(s)	1383
Eco88I	CYCGRG	3 cut(s)	1286, 1795, 1872
Eco91I	GGTNACC	1 cut(s)	1349
EcoO65I	GGTNACC	1 cut(s)	1349
EcoRII	CCWGG	3 cut(s)	513, 1150, 1172
EcoT14I	CCWWGG	2 cut(s)	748, 1063
EcoT38I	GRGCYC	1 cut(s)	1743
ErhI	CCWWGG	2 cut(s)	748, 1063
FalI	AAGNNNNNCTT	4 cut(s)	126, 158, 251, 283
FaqI	GGGAC	2 cut(s)	1876, 1936
FauNDI	CATATG	1 cut(s)	1698
FbaI	TGATCA	2 cut(s)	493, 640
FblI	GTMKAC	1 cut(s)	1396
Fnu4HI	GCNGC	6 cut(s)	191, 543, 623, 756, 759, 1041
FokI	GGATG	2 cut(s)	97, 360
FriOI	GRGCYC	1 cut(s)	1743
Fsp4HI	GCNGC	6 cut(s)	191, 543, 623, 756, 759, 1041
FspBI	CTAG	3 cut(s)	1056, 1509, 1545
GluI	GCNGC	6 cut(s)	191, 543, 623, 756, 759, 1041
GsaI	CCCAGC	2 cut(s)	968, 1863
GsuI	CTGGAG	2 cut(s)	374, 497
HaeIII	GGCC	2 cut(s)	246, 1904
HapII	CCGG	2 cut(s)	55, 1905
HgaI	GACGC	1 cut(s)	1023
Hin1I	GRCGYC	1 cut(s)	1034
HincII	GTYRAC	4 cut(s)	558, 985, 1397, 1780
HindII	GTYRAC	4 cut(s)	558, 985, 1397, 1780
HindIII	AAGCTT	3 cut(s)	228, 260, 1124
HinfI	GANTC	5 cut(s)	339, 370, 530, 1388, 1505
HpaI	GTTAAC	2 cut(s)	558, 985
HpaII	CCGG	2 cut(s)	55, 1905
HphI	GGTGA	4 cut(s)	292, 334, 1361, 1930
Hpy166II	GTNNAC	8 cut(s)	550, 558, 682, 985, 1010, 1276, 1397, 1780
Hpy188I	TCNGA	7 cut(s)	99, 258, 579, 645, 1017, 1504, 1660
Hpy8I	GTNNAC	8 cut(s)	550, 558, 682, 985, 1010, 1276, 1397, 1780
Hpy99I	CGWCG	1 cut(s)	1398
HpyAV	CCTTC	2 cut(s)	1244, 1766
HpyCH4III	ACNGT	3 cut(s)	766, 939, 1585
HpyCH4IV	ACGT	2 cut(s)	686, 1728
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1040, 1620
HpyF3I	CTNAG	3 cut(s)	676, 1383, 1735
HpySE526I	ACGT	2 cut(s)	686, 1728
Hsp92I	GRCGYC	1 cut(s)	1034
Ksp22I	TGATCA	2 cut(s)	493, 640
KspAI	GTTAAC	2 cut(s)	558, 985
Kzo9I	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
LguI	GCTCTTC	2 cut(s)	189, 510
LmnI	GCTCC	6 cut(s)	44, 393, 431, 785, 1384, 1611
Lsp1109I	GCAGC	6 cut(s)	202, 554, 609, 742, 770, 1052
LweI	GCATC	4 cut(s)	75, 169, 1270, 1876
MaeI	CTAG	3 cut(s)	1056, 1509, 1545
MaeII	ACGT	2 cut(s)	686, 1728
MaeIII	GTNAC	4 cut(s)	660, 1349, 1516, 1785
MalI	GATC	8 cut(s)	252, 495, 519, 642, 918, 1202, 1255, 1417
MboI	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
MfeI	CAATTG	1 cut(s)	1454
MflI	RGATCY	3 cut(s)	517, 1200, 1253
MhlI	GDGCHC	2 cut(s)	1063, 1743
MluCI	AATT	7 cut(s)	33, 562, 922, 1410, 1454, 1557, 1644
MmeI	TCCRAC	2 cut(s)	22, 105
MroXI	GAANNNNTTC	2 cut(s)	314, 686
MseI	TTAA	8 cut(s)	36, 402, 557, 921, 984, 1187, 1299, 1580
MslI	CAYNNNNRTG	1 cut(s)	1685
MspA1I	CMGCKG	1 cut(s)	545
MspI	CCGG	2 cut(s)	55, 1905
MspR9I	CCNGG	3 cut(s)	515, 1152, 1174
MunI	CAATTG	1 cut(s)	1454
Mva1269I	GAATGC	1 cut(s)	289
MvaI	CCWGG	3 cut(s)	515, 1152, 1174
MwoI	GCNNNNNNNGC	2 cut(s)	1040, 1620
NdeI	CATATG	1 cut(s)	1698
NdeII	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
NlaIV	GGNNCC	2 cut(s)	519, 1255
NmuCI	GTSAC	3 cut(s)	660, 1349, 1785
NspI	RCATGY	6 cut(s)	65, 410, 599, 1030, 1077, 1630
PaeI	GCATGC	1 cut(s)	1030
PaeR7I	CTCGAG	3 cut(s)	1286, 1795, 1872
PagI	TCATGA	1 cut(s)	451
PaqCI	CACCTGC	1 cut(s)	1994
PciI	ACATGT	1 cut(s)	1626
PciSI	GCTCTTC	2 cut(s)	189, 510
PcsI	WCGNNNNNNNCGW	2 cut(s)	1284, 1962
PctI	GAATGC	1 cut(s)	289
PdmI	GAANNNNTTC	2 cut(s)	314, 686
PfeI	GAWTC	5 cut(s)	339, 370, 530, 1388, 1505
PfoI	TCCNGGA	1 cut(s)	513
PkrI	GCNGC	6 cut(s)	192, 544, 624, 757, 760, 1042
PscI	ACATGT	1 cut(s)	1626
PshAI	GACNNNNGTC	1 cut(s)	110
Psp6I	CCWGG	3 cut(s)	513, 1150, 1172
PspEI	GGTNACC	1 cut(s)	1349
PspFI	CCCAGC	2 cut(s)	964, 1859
PspGI	CCWGG	3 cut(s)	513, 1150, 1172
PspN4I	GGNNCC	2 cut(s)	519, 1255
PspPI	GGNCC	3 cut(s)	244, 463, 1079
PspXI	VCTCGAGB	1 cut(s)	1872
PstI	CTGCAG	2 cut(s)	192, 760
PstNI	CAGNNNCTG	1 cut(s)	1859
PsuI	RGATCY	3 cut(s)	517, 1200, 1253
PvuII	CAGCTG	1 cut(s)	545
RsaI	GTAC	5 cut(s)	936, 1011, 1245, 1712, 1767
RsaNI	GTAC	5 cut(s)	935, 1010, 1244, 1711, 1766
RseI	CAYNNNNRTG	1 cut(s)	1685
SalI	GTCGAC	1 cut(s)	1395
SapI	GCTCTTC	2 cut(s)	189, 510
SaqAI	TTAA	8 cut(s)	36, 402, 557, 921, 984, 1187, 1299, 1580
SatI	GCNGC	6 cut(s)	191, 543, 623, 756, 759, 1041
Sau3AI	GATC	8 cut(s)	250, 493, 517, 640, 916, 1200, 1253, 1415
Sau96I	GGNCC	3 cut(s)	244, 463, 1079
ScaI	AGTACT	1 cut(s)	1767
ScrFI	CCNGG	3 cut(s)	515, 1152, 1174
SduI	GDGCHC	2 cut(s)	1063, 1743
SfaNI	GCATC	4 cut(s)	75, 169, 1270, 1876
SfcI	CTRYAG	4 cut(s)	188, 723, 756, 1167
Sfr274I	CTCGAG	3 cut(s)	1286, 1795, 1872
SinI	GGWCC	2 cut(s)	463, 1079
SlaI	CTCGAG	3 cut(s)	1286, 1795, 1872
SmiMI	CAYNNNNRTG	1 cut(s)	1685
SmlI	CTYRAG	4 cut(s)	1286, 1475, 1795, 1872
SmoI	CTYRAG	4 cut(s)	1286, 1475, 1795, 1872
SphI	GCATGC	1 cut(s)	1030
Sse9I	AATT	7 cut(s)	33, 562, 922, 1410, 1454, 1557, 1644
SsiI	CCGC	2 cut(s)	1024, 1419
SspI	AATATT	2 cut(s)	213, 1600
SspMI	CTAG	3 cut(s)	1056, 1509, 1545
StyD4I	CCNGG	3 cut(s)	513, 1150, 1172
StyI	CCWWGG	2 cut(s)	748, 1063
TaaI	ACNGT	3 cut(s)	766, 939, 1585
TaiI	ACGT	2 cut(s)	689, 1731
TaqI	TCGA	8 cut(s)	318, 926, 1287, 1396, 1414, 1796, 1811, 1873
TaqII	GACCGA	2 cut(s)	480, 831
TasI	AATT	7 cut(s)	33, 562, 922, 1410, 1454, 1557, 1644
TatI	WGTACW	2 cut(s)	1243, 1765
TfiI	GAWTC	5 cut(s)	339, 370, 530, 1388, 1505
Tru1I	TTAA	8 cut(s)	36, 402, 557, 921, 984, 1187, 1299, 1580
Tru9I	TTAA	8 cut(s)	36, 402, 557, 921, 984, 1187, 1299, 1580
TscAI	CASTG	1 cut(s)	822
TseFI	GTSAC	3 cut(s)	660, 1349, 1785
TseI	GCWGC	6 cut(s)	190, 542, 622, 755, 758, 1040
Tsp45I	GTSAC	3 cut(s)	660, 1349, 1785
TspDTI	ATGAA	7 cut(s)	17, 440, 468, 1245, 1356, 1380, 1459
TspGWI	ACGGA	2 cut(s)	1698, 1938
TspRI	CASTG	1 cut(s)	822
VpaK11BI	GGWCC	2 cut(s)	463, 1079
XceI	RCATGY	6 cut(s)	65, 410, 599, 1030, 1077, 1630
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1897
XhoI	CTCGAG	3 cut(s)	1286, 1795, 1872
XmiI	GTMKAC	1 cut(s)	1396
XmnI	GAANNNNTTC	2 cut(s)	314, 686
XspI	CTAG	3 cut(s)	1056, 1509, 1545
ZrmI	AGTACT	1 cut(s)	1767

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.