Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001216

Physical Location & Seq

Genomic Coordinates Forward (+)

9395159 .. 9401627

Gene Structure 6469 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1068900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2115 bp

ATGGCTTTTGAGAAAATGGTTGCCTTTGGGAAAAAGCTGAAGGAAAGACAAGTACAAGAATGGCAGGGGTATTACATCAACTATAAACTTATGAAGAAAAAAGTGAAACACTATTCTCAACAAATTAAAGTTGGAGCAATAGACCGGAGGCACGTCCTAAAGGACTTTTCAAGAATGCTGGATAATCAGATTGAGACTATCGTCCTTTTCCTTTTGGAACAACAAGGACTACTTGCAAGCAGGATAGCCAAGCTTGGAGAACAGCATGAAGCTCTTCAGCAGCAACGTGATCTATCCAAAATATCTGAACTACGAGAAGCTTATAGACAAGTGGGGAGTGATCTTCTAAAGCTTCTGTTTTTTGTTGAGGTGAATGCCATAGGTCTGCGTAAGATACTGAAGAAGTTCGATAAACGCTTTGGGTATAGATTTACTGACTACTACGTCAAAACTCGTGCGAATCATCCTTACTCACAGCTGCAGCAAGTGTTTAAGCATGTGGGCATAGGGGCTGTTGTAGGAGCTATATCTCGCAATCTTCATGAACTTCAGGACCGACAAGGAAGCTACTTATCAATATATGATCAGCCTGCTCTTCCCCTCCAGGATCCTGTCATTGACTCTGTAAAAGCAGCTGTGGACAGGTTAACCAATTCAACAAACTTTCTTAACTTTTTGGGACAACATGCACTTATTCTGCAAGAAGATGTGCTGCCTGCTCCAGTTGAGGAAGAAGTTGATGATCAGAGATACCACTTTATGTCACTTGTCTTAAACTTAGTGAACACGTTCCTTTACATGGTCAATACATATATTATTGTTCCTACAGCAGATGACTACTCGATGAGCCTTGGTGCTGCAGCAACAGTTTGTGGTGTTGTGATTGGAGCAATGGCTGTTGCACAGGTGTTTTCTTCAGTGTATTTTAGTGCATGGTCAAATCGATCATACTTCAAGCCTCTTGTATTTAGCAGTATTGTTCTTTTTGCGGGAAACACCCTGTATGCTCTGGCTTATGATCTTAGTTCGATATGGGTACTGTTGATTGGTCGTCTTTTCTGTGGGCTGGGTTCTGCAAGAGCTGTTAACAGGCGTTATATTAGTGATTGTGTACCTCTGAAAATCCGCATGCAGGCATCAGCAGCTTTTGTTAGTGCTAGTGCCCTTGGAATGGCATGTGGTCCTGCTCTTGCTGGCTTACTTCAAACTAATTTCAAGATTTACAAGCTTACATTCAATCAAGACACTTTGCCTGGTTGGGTTATGGCTATAGCTTGGCTACTATACTTGATATGGGTGTGCATCTCATTCAAAGAACCTGCTCGTGAAGATGAAGTGAATCCTGTACAAGAGGGATCTAATAGCGGAACAGCAGTAAATGATGCACTCGAGAAAGGTCTTCAACAACCATTACTCATTAGTGCTGAAGACAAGAATGAAGATGAAGATGGTGACCAAGATATTGATGAAAGTGAAGAAGCTCCTGAGGAATCTCGTCAACCAGCCACTTCAATTCGATCCGCATATCGACTACTTACACCCTCTGTAAAGGTTCAACTGTTGATATATTTCATGCTCAAGTATGCTGTGGAGATTTTAATTTCAGAATCTAGTGTTGTTACCACATACTACTTTAGCTGGAATACCAGCAAAGTCGCAATTTTTCTGGCATGTCTTGGATTAACCGTTCTTCCAGTAAATATTCTTGTTGGGAGCTACATCAGCAACATGTTTGAAGACAGGCAAATTTTACTAGTATCCGAAATAGTGGTTTGCATAGGCATAGTTATGAGCTTCCATATGTTTAATCCGTACTCGGTGATACAATACGTCAGCTCAGGGCTCATCATGTTTGTTTCAGCTGAAGTACTTGAAGGTGTCAACTTGTCACTTCTCTCTAGGGTCATGTCATCGAGGCTTTCCCGCGGAACATACAATGGTGGATTACTTTCAACAGAAGCTGGGACGCTCGCTCGAGTAGTAGCAGATGCCACCATAACTCTAGCCGGATACTTGGGGGAGAGTAAGCTCTTGAACGTCACCTTGCTTCCTTCCCTCGTGATCTGCATTACTTCCATTATTGCCACCTGCTACACGTACAACTCTCTCTATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006817 GO:0006820 GO:0008150 GO:0009705 GO:0015698 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0051179 GO:0051234 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

MFS_1 SPX

Protein Analysis

705

Amino Acids

78.72

Weight (kDa)

6.36

Isoelectric Point (pI)

39.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
SPX	PF03105	9 - 42	7.6e-06	SPX domain
SPX	PF03105	114 - 151	1.6e-06	SPX domain
MFS_1	PF07690	261 - 647	1.2e-25	Major Facilitator Superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001216)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT1G63010 AT4G11810 AT4G11810 AT4G11810 AT4G22990 AT4G22990 AT4G22990
fragaria_vesca	FvH4_5g01390 FvH4_7g12920 FvH4_7g12920 FvH4_7g12920
malus_domestica	MD02G1195400.v1.1 MD04G1068900.v1.1 MD06G1069900.v1.1
prunus_persica	Prupe.2G153100_v2.0.a1 Prupe.2G153100_v2.0.a1 Prupe.2G153100_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1 Prupe.5G089200_v2.0.a1
pyrus_communis	pycom02g15930 pycom04g06270 pycom06g07090
rosa_chinensis	RchiOBHm_Chr1g0352001 RchiOBHm_Chr7g0199491
rosa_laevigata	RLG00000003789 RLG00000028368
rosa_multiflora	Rmu_sc0003549.1_g000001 Rmu_sc0004009.1_g000006 Rmu_sc0007728.1_g000012
rosa_roxburghii	Rroxscaffold_3G00256680 Rroxscaffold_4G00303360
rosa_rugosa	Rorug01G0223300 Rorug01G0223400 Rorug07G0054600 Rorug07G0054700 Rorug07G0054800 Rorug07G0054900
rosa_samantha	Rh1AG237400 Rh1BG207600 Rh1CG221800 Rh1DG234600 Rh7AG179600 Rh7BG182900 Rh7CG188200 Rh7DG182300
rosa_wichuraiana	Rw1G020650 Rw7G015770

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	2096
AasI	GACNNNNNNGTC	1 cut(s)	443
Acc36I	ACCTGC	2 cut(s)	1327, 2096
AccII	CGCG	1 cut(s)	1926
AciI	CCGC	6 cut(s)	989, 1126, 1365, 1521, 1924, 1926
AclWI	GGATC	4 cut(s)	602, 615, 1363, 1512
AcsI	RAATTY	1 cut(s)	1746
AcuI	CTGAAG	7 cut(s)	59, 260, 419, 533, 900, 1446, 1884
AfaI	GTAC	7 cut(s)	54, 1038, 1113, 1347, 1814, 1869, 2099
AfiI	CCNNNNNNNGG	3 cut(s)	799, 1132, 1171
AflIII	ACRYGT	3 cut(s)	786, 1728, 2094
AhlI	ACTAGT	1 cut(s)	1753
AjiI	CACGTC	1 cut(s)	154
AjnI	CCWGG	2 cut(s)	603, 1252
AloI	GAACNNNNNNTCC	2 cut(s)	1671, 1703
Alw26I	GTCTC	1 cut(s)	188
AlwI	GGATC	4 cut(s)	602, 615, 1363, 1512
AlwNI	CAGNNNCTG	1 cut(s)	1961
Ama87I	CYCGRG	2 cut(s)	1388, 1974
ApeKI	GCWGC	8 cut(s)	280, 478, 481, 632, 712, 857, 860, 1142
ApoI	RAATTY	1 cut(s)	1746
Asp700I	GAANNNNTTC	3 cut(s)	273, 404, 788
AspS9I	GGNCC	2 cut(s)	553, 1181
AsuHPI	GGTGA	4 cut(s)	382, 1463, 1831, 2032
AvaI	CYCGRG	2 cut(s)	1388, 1974
AvaII	GGWCC	2 cut(s)	553, 1181
AxyI	CCTNAGG	1 cut(s)	1485
BaeGI	GKGCMC	1 cut(s)	1165
BamHI	GGATCC	1 cut(s)	607
BanII	GRGCYC	1 cut(s)	1845
BauI	CACGAG	3 cut(s)	453, 1323, 2057
BbsI	GAAGAC	3 cut(s)	1391, 1434, 1743
BbvI	GCAGC	8 cut(s)	292, 465, 493, 644, 699, 844, 872, 1154
BccI	CCATC	1 cut(s)	1442
BciT130I	CCWGG	2 cut(s)	605, 1254
BciVI	GTATCC	2 cut(s)	1768, 2003
BclI	TGATCA	2 cut(s)	583, 742
BcoDI	GTCTC	1 cut(s)	188
BcuI	ACTAGT	1 cut(s)	1753
BfaI	CTAG	5 cut(s)	1158, 1611, 1754, 1899, 2003
BfmI	CTRYAG	4 cut(s)	479, 825, 858, 1269
BfuAI	ACCTGC	2 cut(s)	1327, 2096
BfuI	GTATCC	2 cut(s)	1768, 2003
BisI	GCNGC	8 cut(s)	281, 479, 482, 633, 713, 858, 861, 1143
BlsI	GCNGC	8 cut(s)	282, 480, 483, 634, 714, 859, 862, 1144
BmcAI	AGTACT	1 cut(s)	1869
Bme1390I	CCNGG	2 cut(s)	605, 1254
Bme18I	GGWCC	2 cut(s)	553, 1181
BmeT110I	CYCGRG	2 cut(s)	1388, 1974
BmgBI	CACGTC	1 cut(s)	154
BmgT120I	GGNCC	2 cut(s)	553, 1181
BmiI	GGNNCC	1 cut(s)	609
BmrFI	CCNGG	2 cut(s)	605, 1254
BmsI	GCATC	4 cut(s)	1145, 1311, 1372, 1978
BoxI	GACNNNNGTC	1 cut(s)	200
BpiI	GAAGAC	3 cut(s)	1391, 1434, 1743
BpmI	CTGGAG	2 cut(s)	587, 705
Bpu10I	CCTNAGC	1 cut(s)	1837
BpuEI	CTTGAG	1 cut(s)	1562
Bsa29I	ATCGAT	1 cut(s)	943
BsaAI	YACGTR	1 cut(s)	2097
BsaJI	CCNNGG	3 cut(s)	850, 1165, 1924
BsaWI	WCCGGW	1 cut(s)	144
Bsc4I	CCNNNNNNNGG	3 cut(s)	799, 1132, 1171
Bse1I	ACTGG	2 cut(s)	722, 1694
Bse21I	CCTNAGG	1 cut(s)	1485
Bse3DI	GCAATG	1 cut(s)	897
BseBI	CCWGG	2 cut(s)	605, 1254
BseCI	ATCGAT	1 cut(s)	943
BseDI	CCNNGG	3 cut(s)	850, 1165, 1924
BseGI	GGATG	1 cut(s)	463
BseLI	CCNNNNNNNGG	3 cut(s)	799, 1132, 1171
BseMI	GCAATG	1 cut(s)	897
BseMII	CTCAG	2 cut(s)	1476, 1851
BseNI	ACTGG	2 cut(s)	722, 1694
BseSI	GKGCMC	1 cut(s)	1165
BseXI	GCAGC	8 cut(s)	292, 465, 493, 644, 699, 844, 872, 1154
BseYI	CCCAGC	2 cut(s)	1066, 1961
Bsh1236I	CGCG	1 cut(s)	1926
BshVI	ATCGAT	1 cut(s)	943
BsiHKCI	CYCGRG	2 cut(s)	1388, 1974
BsiSI	CCGG	2 cut(s)	145, 2007
BslFI	GGGAC	2 cut(s)	693, 1978
BslI	CCNNNNNNNGG	3 cut(s)	799, 1132, 1171
BsmAI	GTCTC	1 cut(s)	188
BsmFI	GGGAC	2 cut(s)	693, 1978
BsmI	GAATGC	2 cut(s)	180, 379
BsoBI	CYCGRG	2 cut(s)	1388, 1974
Bsp1286I	GDGCHC	2 cut(s)	1165, 1845
Bsp1407I	TGTACA	1 cut(s)	1345
BspACI	CCGC	6 cut(s)	989, 1126, 1365, 1521, 1924, 1926
BspCNI	CTCAG	2 cut(s)	1477, 1850
BspDI	ATCGAT	1 cut(s)	943
BspFNI	CGCG	1 cut(s)	1926
BspHI	TCATGA	1 cut(s)	541
BspLI	GGNNCC	1 cut(s)	609
BspMAI	CTGCAG	2 cut(s)	483, 862
BspMI	ACCTGC	2 cut(s)	1327, 2096
BspPI	GGATC	4 cut(s)	602, 615, 1363, 1512
BspQI	GCTCTTC	2 cut(s)	279, 600
BsrDI	GCAATG	1 cut(s)	897
BsrGI	TGTACA	1 cut(s)	1345
BsrI	ACTGG	2 cut(s)	722, 1694
BssECI	CCNNGG	3 cut(s)	850, 1165, 1924
BssSI	CACGAG	3 cut(s)	453, 1323, 2057
BssT1I	CCWWGG	2 cut(s)	850, 1165
Bst2BI	CACGAG	3 cut(s)	453, 1323, 2057
Bst2UI	CCWGG	2 cut(s)	605, 1254
Bst4CI	ACNGT	4 cut(s)	868, 1041, 1560, 1687
Bst6I	CTCTTC	2 cut(s)	279, 600
BstAUI	TGTACA	1 cut(s)	1345
BstBAI	YACGTR	1 cut(s)	2097
BstC8I	GCNNGC	7 cut(s)	238, 591, 717, 1130, 1134, 1195, 1971
BstDEI	CTNAG	4 cut(s)	778, 1022, 1485, 1837
BstDSI	CCRYGG	1 cut(s)	1924
BstEII	GGTNACC	1 cut(s)	1451
BstF5I	GGATG	1 cut(s)	463
BstFNI	CGCG	1 cut(s)	1926
BstMAI	GTCTC	1 cut(s)	188
BstMWI	GCNNNNNNNGC	2 cut(s)	1142, 1722
BstNI	CCWGG	2 cut(s)	605, 1254
BstNSI	RCATGY	6 cut(s)	500, 689, 1132, 1179, 1674, 1732
BstPAI	GACNNNNGTC	1 cut(s)	200
BstPI	GGTNACC	1 cut(s)	1451
BstSCI	CCNGG	2 cut(s)	603, 1252
BstSFI	CTRYAG	4 cut(s)	479, 825, 858, 1269
BstSLI	GKGCMC	1 cut(s)	1165
BstUI	CGCG	1 cut(s)	1926
BstV1I	GCAGC	8 cut(s)	292, 465, 493, 644, 699, 844, 872, 1154
BstV2I	GAAGAC	3 cut(s)	1391, 1434, 1743
BstX2I	RGATCY	2 cut(s)	607, 1355
BstYI	RGATCY	2 cut(s)	607, 1355
Bsu15I	ATCGAT	1 cut(s)	943
Bsu36I	CCTNAGG	1 cut(s)	1485
BsuI	GTATCC	2 cut(s)	1768, 2003
BsuTUI	ATCGAT	1 cut(s)	943
BtgI	CCRYGG	1 cut(s)	1924
BtrI	CACGTC	1 cut(s)	154
BtsCI	GGATG	1 cut(s)	463
BtsIMutI	CAGTG	1 cut(s)	924
BveI	ACCTGC	2 cut(s)	1327, 2096
Cac8I	GCNNGC	7 cut(s)	238, 591, 717, 1130, 1134, 1195, 1971
CaiI	CAGNNNCTG	1 cut(s)	1961
CciI	TCATGA	1 cut(s)	541
Cfr13I	GGNCC	2 cut(s)	553, 1181
Cfr42I	CCGCGG	1 cut(s)	1927
ClaI	ATCGAT	1 cut(s)	943
CseI	GACGC	1 cut(s)	1975
Csp6I	GTAC	7 cut(s)	53, 1037, 1112, 1346, 1813, 1868, 2098
CviQI	GTAC	7 cut(s)	53, 1037, 1112, 1346, 1813, 1868, 2098
DdeI	CTNAG	4 cut(s)	778, 1022, 1485, 1837
DrdI	GACNNNNNNGTC	1 cut(s)	443
DseDI	GACNNNNNNGTC	1 cut(s)	443
Eam1104I	CTCTTC	2 cut(s)	279, 600
EarI	CTCTTC	2 cut(s)	279, 600
Eco130I	CCWWGG	2 cut(s)	850, 1165
Eco24I	GRGCYC	1 cut(s)	1845
Eco47I	GGWCC	2 cut(s)	553, 1181
Eco57I	CTGAAG	7 cut(s)	59, 260, 419, 533, 900, 1446, 1884
Eco81I	CCTNAGG	1 cut(s)	1485
Eco88I	CYCGRG	2 cut(s)	1388, 1974
Eco91I	GGTNACC	1 cut(s)	1451
EcoO65I	GGTNACC	1 cut(s)	1451
EcoRII	CCWGG	2 cut(s)	603, 1252
EcoT14I	CCWWGG	2 cut(s)	850, 1165
EcoT38I	GRGCYC	1 cut(s)	1845
ErhI	CCWWGG	2 cut(s)	850, 1165
FalI	AAGNNNNNCTT	2 cut(s)	216, 248
FaqI	GGGAC	2 cut(s)	693, 1978
FauI	CCCGC	2 cut(s)	982, 1931
FauNDI	CATATG	1 cut(s)	1800
FbaI	TGATCA	2 cut(s)	583, 742
Fnu4HI	GCNGC	8 cut(s)	281, 479, 482, 633, 713, 858, 861, 1143
FokI	GGATG	1 cut(s)	450
FriOI	GRGCYC	1 cut(s)	1845
Fsp4HI	GCNGC	8 cut(s)	281, 479, 482, 633, 713, 858, 861, 1143
FspBI	CTAG	5 cut(s)	1158, 1611, 1754, 1899, 2003
GluI	GCNGC	8 cut(s)	281, 479, 482, 633, 713, 858, 861, 1143
GsaI	CCCAGC	2 cut(s)	1070, 1965
GsuI	CTGGAG	2 cut(s)	587, 705
HapII	CCGG	2 cut(s)	145, 2007
HgaI	GACGC	1 cut(s)	1975
HincII	GTYRAC	4 cut(s)	648, 1087, 1499, 1882
HindII	GTYRAC	4 cut(s)	648, 1087, 1499, 1882
HindIII	AAGCTT	4 cut(s)	251, 318, 350, 1226
HinfI	GANTC	5 cut(s)	460, 620, 1339, 1490, 1607
HpaI	GTTAAC	2 cut(s)	648, 1087
HpaII	CCGG	2 cut(s)	145, 2007
HphI	GGTGA	4 cut(s)	382, 1463, 1831, 2032
Hpy166II	GTNNAC	7 cut(s)	640, 648, 784, 1087, 1112, 1499, 1882
Hpy188I	TCNGA	6 cut(s)	189, 307, 747, 1119, 1606, 1762
Hpy8I	GTNNAC	7 cut(s)	640, 648, 784, 1087, 1112, 1499, 1882
HpyAV	CCTTC	3 cut(s)	34, 1868, 2061
HpyCH4III	ACNGT	4 cut(s)	868, 1041, 1560, 1687
HpyCH4IV	ACGT	7 cut(s)	153, 286, 444, 788, 1830, 2037, 2096
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1142, 1722
HpyF3I	CTNAG	4 cut(s)	778, 1022, 1485, 1837
HpySE526I	ACGT	7 cut(s)	153, 286, 444, 788, 1830, 2037, 2096
Ksp22I	TGATCA	2 cut(s)	583, 742
KspAI	GTTAAC	2 cut(s)	648, 1087
KspI	CCGCGG	1 cut(s)	1927
LguI	GCTCTTC	2 cut(s)	279, 600
LmnI	GCTCC	6 cut(s)	134, 521, 724, 887, 1486, 1713
Lsp1109I	GCAGC	8 cut(s)	292, 465, 493, 644, 699, 844, 872, 1154
LweI	GCATC	4 cut(s)	1145, 1311, 1372, 1978
MaeI	CTAG	5 cut(s)	1158, 1611, 1754, 1899, 2003
MaeII	ACGT	7 cut(s)	153, 286, 444, 788, 1830, 2037, 2096
MaeIII	GTNAC	5 cut(s)	762, 1451, 1618, 1887, 2038
MflI	RGATCY	2 cut(s)	607, 1355
MhlI	GDGCHC	2 cut(s)	1165, 1845
MluCI	AATT	7 cut(s)	123, 652, 1210, 1512, 1599, 1659, 1746
MlyI	GAGTC	1 cut(s)	614
MmeI	TCCRAC	1 cut(s)	112
MroXI	GAANNNNTTC	3 cut(s)	273, 404, 788
MseI	TTAA	9 cut(s)	126, 492, 647, 669, 773, 1086, 1598, 1682, 1806
MslI	CAYNNNNRTG	1 cut(s)	1787
MspA1I	CMGCKG	4 cut(s)	478, 635, 1862, 1926
MspI	CCGG	2 cut(s)	145, 2007
MspR9I	CCNGG	2 cut(s)	605, 1254
Mva1269I	GAATGC	2 cut(s)	180, 379
MvaI	CCWGG	2 cut(s)	605, 1254
MvnI	CGCG	1 cut(s)	1926
MwoI	GCNNNNNNNGC	2 cut(s)	1142, 1722
NdeI	CATATG	1 cut(s)	1800
NlaIV	GGNNCC	1 cut(s)	609
NmuCI	GTSAC	4 cut(s)	762, 1451, 1887, 2038
NspI	RCATGY	6 cut(s)	500, 689, 1132, 1179, 1674, 1732
PaeI	GCATGC	1 cut(s)	1132
PaeR7I	CTCGAG	2 cut(s)	1388, 1974
PagI	TCATGA	1 cut(s)	541
PaqCI	CACCTGC	1 cut(s)	2096
PciI	ACATGT	1 cut(s)	1728
PciSI	GCTCTTC	2 cut(s)	279, 600
PctI	GAATGC	2 cut(s)	180, 379
PdmI	GAANNNNTTC	3 cut(s)	273, 404, 788
PfeI	GAWTC	4 cut(s)	460, 1339, 1490, 1607
PfoI	TCCNGGA	1 cut(s)	603
PkrI	GCNGC	8 cut(s)	282, 480, 483, 634, 714, 859, 862, 1144
PleI	GAGTC	1 cut(s)	614
PpsI	GAGTC	1 cut(s)	614
Ppu21I	YACGTR	1 cut(s)	2097
PscI	ACATGT	1 cut(s)	1728
PshAI	GACNNNNGTC	1 cut(s)	200
Psp6I	CCWGG	2 cut(s)	603, 1252
PspEI	GGTNACC	1 cut(s)	1451
PspFI	CCCAGC	2 cut(s)	1066, 1961
PspGI	CCWGG	2 cut(s)	603, 1252
PspN4I	GGNNCC	1 cut(s)	609
PspPI	GGNCC	2 cut(s)	553, 1181
PspXI	VCTCGAGB	1 cut(s)	1974
PstI	CTGCAG	2 cut(s)	483, 862
PstNI	CAGNNNCTG	1 cut(s)	1961
PsuI	RGATCY	2 cut(s)	607, 1355
PvuII	CAGCTG	3 cut(s)	478, 635, 1862
RsaI	GTAC	7 cut(s)	54, 1038, 1113, 1347, 1814, 1869, 2099
RsaNI	GTAC	7 cut(s)	53, 1037, 1112, 1346, 1813, 1868, 2098
RseI	CAYNNNNRTG	1 cut(s)	1787
SacII	CCGCGG	1 cut(s)	1927
SapI	GCTCTTC	2 cut(s)	279, 600
SaqAI	TTAA	9 cut(s)	126, 492, 647, 669, 773, 1086, 1598, 1682, 1806
SatI	GCNGC	8 cut(s)	281, 479, 482, 633, 713, 858, 861, 1143
Sau96I	GGNCC	2 cut(s)	553, 1181
ScaI	AGTACT	1 cut(s)	1869
SchI	GAGTC	1 cut(s)	614
ScrFI	CCNGG	2 cut(s)	605, 1254
SduI	GDGCHC	2 cut(s)	1165, 1845
SfaNI	GCATC	4 cut(s)	1145, 1311, 1372, 1978
SfcI	CTRYAG	4 cut(s)	479, 825, 858, 1269
Sfr274I	CTCGAG	2 cut(s)	1388, 1974
Sfr303I	CCGCGG	1 cut(s)	1927
SgrBI	CCGCGG	1 cut(s)	1927
SinI	GGWCC	2 cut(s)	553, 1181
SlaI	CTCGAG	2 cut(s)	1388, 1974
SmiMI	CAYNNNNRTG	1 cut(s)	1787
SmlI	CTYRAG	3 cut(s)	1388, 1577, 1974
SmoI	CTYRAG	3 cut(s)	1388, 1577, 1974
SpeI	ACTAGT	1 cut(s)	1753
SphI	GCATGC	1 cut(s)	1132
Sse9I	AATT	7 cut(s)	123, 652, 1210, 1512, 1599, 1659, 1746
SsiI	CCGC	6 cut(s)	989, 1126, 1365, 1521, 1924, 1926
SspI	AATATT	1 cut(s)	1702
SspMI	CTAG	5 cut(s)	1158, 1611, 1754, 1899, 2003
StyD4I	CCNGG	2 cut(s)	603, 1252
StyI	CCWWGG	2 cut(s)	850, 1165
TaaI	ACNGT	4 cut(s)	868, 1041, 1560, 1687
TaiI	ACGT	7 cut(s)	156, 289, 447, 791, 1833, 2040, 2099
TaqI	TCGA	9 cut(s)	408, 842, 943, 1028, 1389, 1516, 1528, 1913, 1975
TaqII	GACCGA	1 cut(s)	570
TasI	AATT	7 cut(s)	123, 652, 1210, 1512, 1599, 1659, 1746
TatI	WGTACW	3 cut(s)	52, 1345, 1867
TfiI	GAWTC	4 cut(s)	460, 1339, 1490, 1607
Tru1I	TTAA	9 cut(s)	126, 492, 647, 669, 773, 1086, 1598, 1682, 1806
Tru9I	TTAA	9 cut(s)	126, 492, 647, 669, 773, 1086, 1598, 1682, 1806
TscAI	CASTG	1 cut(s)	924
TseFI	GTSAC	4 cut(s)	762, 1451, 1887, 2038
TseI	GCWGC	8 cut(s)	280, 478, 481, 632, 712, 857, 860, 1142
Tsp45I	GTSAC	4 cut(s)	762, 1451, 1887, 2038
TspDTI	ATGAA	9 cut(s)	107, 282, 530, 558, 1347, 1452, 1458, 1482, 1561
TspGWI	ACGGA	1 cut(s)	1800
TspRI	CASTG	1 cut(s)	924
VpaK11BI	GGWCC	2 cut(s)	553, 1181
XapI	RAATTY	1 cut(s)	1746
XceI	RCATGY	6 cut(s)	500, 689, 1132, 1179, 1674, 1732
XhoI	CTCGAG	2 cut(s)	1388, 1974
XmnI	GAANNNNTTC	3 cut(s)	273, 404, 788
XspI	CTAG	5 cut(s)	1158, 1611, 1754, 1899, 2003
ZrmI	AGTACT	1 cut(s)	1869

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.