Rw1G020650

SPX domain-containing membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001216
Physical Location & Seq
Reverse (-)
44240508 .. 44244224
3717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020650.1

Sequence Viewer

Length: 1479 bp
ATGCAGGAGGAGTTTCCAAGTTCACCTGAGGATCCTATTGATGACAGCACATATCATTTCATGTCACTTCTCTTAAATTTGGCAAATACATTTCTCTATATGGTCAATACATATATAATTGTCCCAACAGCTGACAGATACACCATGACCCTTGGAGCCGCAGCAACGGTCTGTGGTGTTGTGATTGGGTCAATGGCAGTTGCCCAGGTGTTTTCTTCAGTTTATTTCAGCGCATGGTCAAATAGATCATACATGAGACCACTTGTATTCAGTAGCATTGTTCTTCTTGTGGGAAATACCCTATATGCACTGGCATATGATCTTGATTCATTATCAGTACTTCTGATTGGTCGACTTTTCTGTGGGTTAGGTTCTGCAAGAGCGGTTAACCGGCGTTATATCAGTGATTGTGTACCCCTTAAATTGCGAATGCAAGCTTCTGCTGGTTTTGTCAGTGCTAGTGCACTCGGAATGGCGTGTGGTCCTGCTCTTGCTTGTTTATTCCAAACTAATTTCAAGATTTACAAGTTGACATTCAATGAAGCCACATTACCTGGTTGGGCCATGGCTCTTGCATGGCTTGTATATTTGCTGTGGTTGTGGATTTCTTTTAGAGAGCCTTCTCGTGAAACTAAAGAAAATGTTGTGCCAGAAGAATCTACTTCTGGAAGGTATATAGATAGTTCTCCTGAAACTAAAGAAAATGCCTTGCCACAGGAATCTAATTCTGGGAGGTTTATAAATATTTCTGTGGACGATAATTCTACACAACCATTACTGATGAACTCCAACGCTAAGCAACAAGATGAAGATGGAGACGAAGACTGTGATGATGCTGAAGAAGATTCCAAAGAACTCCAGAGGCCAGTCAATTCAATTGTGTCAGCATATAGATTACTTACACCATCAGTAAAGGTTCAACTACTTATATATTTTATGCTCAAATATGCTATGGAGATCATACTTGCTGAGTCGAGTCTCATCACTGGATATTATTTTATTTGGTCAACTAGCAGCGTGGCAATATTTCTTGCATGTCTTGGGCTAACAGTGCTTCCAGTCAATGTTCTTGTTGGGAGCTATATCAGCAACATATTTGAAGAAAGGCAGGTTCTATTGGCCTCTGAAGTTCTGGTGTGCATAGGTATACTACTGAGCTTCCACATATTGACCTCTTACTCTGTGCCTCAATATGTCGGCTCAGCCCTTCTAACTTTCGTGTCAGCTGAAGTCCTTGAAGGTGTGAACTTATCACTCCTATCTCGCGTCATGTCATCAAGGCTTTCTCGTGGGACTTTCAATGGCGGATTGCTTTCAACTGAGGCAGGGACCCTGGCCCGGGTAGTTGCAGATGGAACAATAACCCTAGCTGGGTATTTAGGTGTGAGCAAGGTCTTGAATACCACCTTGGTTCCTTCTCTGTTCTTCTGTGTCTGTTCCATTGTTGCCACCTGCTTCACCTACAACTCCCTCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_1 SPX Sugar_tr

Protein Analysis

492

Amino Acids

53.93

Weight (kDa)

4.71

Isoelectric Point (pI)

46.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 29 - 425 5e-21 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 740
AarI CACCTGC 1 cut(s) 1460
Acc36I ACCTGC 2 cut(s) 1099, 1460
AccBSI CCGCTC 1 cut(s) 383
AccI GTMKAC 2 cut(s) 352, 1147
AccII CGCG 1 cut(s) 1266
AciI CCGC 3 cut(s) 159, 383, 1305
AclWI GGATC 2 cut(s) 26, 39
AcsI RAATTY 1 cut(s) 76
AcuI CTGAAG 4 cut(s) 201, 858, 1146, 1248
AfaI GTAC 2 cut(s) 339, 414
AfiI CCNNNNNNNGG 3 cut(s) 1338, 1339, 1371
AgsI TTSAA 9 cut(s) 517, 538, 876, 920, 1100, 1238, 1300, 1317, 1399
AjnI CCWGG 3 cut(s) 204, 553, 1332
AjuI GAANNNNNNNTTGG 2 cut(s) 1391, 1423
AluBI AGCT 6 cut(s) 131, 437, 1080, 1158, 1226, 1370
AluI AGCT 6 cut(s) 131, 437, 1080, 1158, 1226, 1370
Alw21I GWGCWC 1 cut(s) 466
Alw26I GTCTC 3 cut(s) 250, 810, 983
Alw44I GTGCAC 1 cut(s) 462
AlwI GGATC 2 cut(s) 26, 39
Ama87I CYCGRG 1 cut(s) 1338
AoxI GGCC 4 cut(s) 561, 863, 1119, 1335
ApaLI GTGCAC 1 cut(s) 462
ApeKI GCWGC 2 cut(s) 161, 1014
ApoI RAATTY 1 cut(s) 76
AspLEI GCGC 1 cut(s) 233
AspS9I GGNCC 4 cut(s) 482, 561, 1329, 1336
AsuC2I CCSGG 2 cut(s) 1339, 1340
AsuHPI GGTGA 2 cut(s) 15, 1450
AvaI CYCGRG 1 cut(s) 1338
AvaII GGWCC 2 cut(s) 482, 1329
AxyI CCTNAGG 1 cut(s) 27
BaeGI GKGCMC 1 cut(s) 466
BamHI GGATCC 1 cut(s) 31
BarI GAAGNNNNNNTAC 2 cut(s) 534, 566
BauI CACGAG 2 cut(s) 624, 1287
BbsI GAAGAC 1 cut(s) 828
Bbv12I GWGCWC 1 cut(s) 466
BbvI GCAGC 2 cut(s) 173, 1026
BccI CCATC 3 cut(s) 806, 913, 1346
BciT130I CCWGG 3 cut(s) 206, 555, 1334
BcnI CCSGG 2 cut(s) 1339, 1340
BcoDI GTCTC 3 cut(s) 250, 810, 983
BfaI CTAG 3 cut(s) 459, 1011, 1367
BfuAI ACCTGC 2 cut(s) 1099, 1460
BisI GCNGC 3 cut(s) 159, 162, 1015
BlpI GCTNAGC 2 cut(s) 795, 1201
BlsI GCNGC 3 cut(s) 160, 163, 1016
BmcAI AGTACT 1 cut(s) 339
Bme1390I CCNGG 5 cut(s) 206, 555, 1334, 1339, 1340
Bme18I GGWCC 2 cut(s) 482, 1329
BmeT110I CYCGRG 1 cut(s) 1338
BmgT120I GGNCC 4 cut(s) 482, 561, 1329, 1336
BmiI GGNNCC 5 cut(s) 33, 157, 1330, 1331, 1413
BmrFI CCNGG 5 cut(s) 206, 555, 1334, 1339, 1340
BmsI GCATC 1 cut(s) 823
BpiI GAAGAC 1 cut(s) 828
BpmI CTGGAG 1 cut(s) 842
Bpu1102I GCTNAGC 2 cut(s) 795, 1201
BpuMI CCSGG 2 cut(s) 1339, 1340
BsaI GGTCTC 1 cut(s) 250
BsaJI CCNNGG 6 cut(s) 151, 204, 564, 1332, 1338, 1407
Bsc4I CCNNNNNNNGG 3 cut(s) 1338, 1339, 1371
Bse118I RCCGGY 1 cut(s) 390
Bse1I ACTGG 4 cut(s) 315, 866, 991, 1058
Bse21I CCTNAGG 1 cut(s) 27
BseBI CCWGG 3 cut(s) 206, 555, 1334
BseDI CCNNGG 6 cut(s) 151, 204, 564, 1332, 1338, 1407
BseLI CCNNNNNNNGG 3 cut(s) 1338, 1339, 1371
BseMII CTCAG 5 cut(s) 18, 960, 1145, 1215, 1311
BseNI ACTGG 4 cut(s) 315, 866, 991, 1058
BseRI GAGGAG 1 cut(s) 23
BseSI GKGCMC 1 cut(s) 466
BseXI GCAGC 2 cut(s) 173, 1026
BseYI CCCAGC 1 cut(s) 1370
Bsh1236I CGCG 1 cut(s) 1266
BshFI GGCC 4 cut(s) 563, 865, 1121, 1337
BsiHKAI GWGCWC 1 cut(s) 466
BsiHKCI CYCGRG 1 cut(s) 1338
BsiSI CCGG 2 cut(s) 391, 1339
BslFI GGGAC 3 cut(s) 107, 1306, 1342
BslI CCNNNNNNNGG 3 cut(s) 1338, 1339, 1371
BsmAI GTCTC 3 cut(s) 250, 810, 983
BsmBI CGTCTC 1 cut(s) 810
BsmFI GGGAC 3 cut(s) 107, 1306, 1342
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 4 cut(s) 563, 865, 1121, 1337
Bso31I GGTCTC 1 cut(s) 250
BsoBI CYCGRG 1 cut(s) 1338
Bsp1286I GDGCHC 1 cut(s) 466
Bsp143I GATC 4 cut(s) 31, 245, 319, 957
Bsp1720I GCTNAGC 2 cut(s) 795, 1201
Bsp19I CCATGG 1 cut(s) 564
BspACI CCGC 3 cut(s) 159, 383, 1305
BspANI GGCC 4 cut(s) 563, 865, 1121, 1337
BspCNI CTCAG 5 cut(s) 19, 961, 1146, 1214, 1312
BspFNI CGCG 1 cut(s) 1266
BspLI GGNNCC 5 cut(s) 33, 157, 1330, 1331, 1413
BspMI ACCTGC 2 cut(s) 1099, 1460
BspPI GGATC 2 cut(s) 26, 39
BspTNI GGTCTC 1 cut(s) 250
BsrBI CCGCTC 1 cut(s) 383
BsrFI RCCGGY 1 cut(s) 390
BsrI ACTGG 4 cut(s) 315, 866, 991, 1058
BssAI RCCGGY 1 cut(s) 390
BssECI CCNNGG 6 cut(s) 151, 204, 564, 1332, 1338, 1407
BssMI GATC 4 cut(s) 31, 245, 319, 957
BssNAI GTATAC 1 cut(s) 1148
BssSI CACGAG 2 cut(s) 624, 1287
BssT1I CCWWGG 3 cut(s) 151, 564, 1407
Bst1107I GTATAC 1 cut(s) 1148
Bst2BI CACGAG 2 cut(s) 624, 1287
Bst2UI CCWGG 3 cut(s) 206, 555, 1334
Bst4CI ACNGT 3 cut(s) 169, 827, 1051
BstC8I GCNNGC 1 cut(s) 435
BstDEI CTNAG 6 cut(s) 27, 795, 969, 1154, 1201, 1320
BstDSI CCRYGG 1 cut(s) 564
BstFNI CGCG 1 cut(s) 1266
BstHHI GCGC 1 cut(s) 233
BstKTI GATC 4 cut(s) 34, 248, 322, 960
BstMAI GTCTC 3 cut(s) 250, 810, 983
BstMBI GATC 4 cut(s) 31, 245, 319, 957
BstMWI GCNNNNNNNGC 2 cut(s) 1051, 1086
BstNI CCWGG 3 cut(s) 206, 555, 1334
BstNSI RCATGY 1 cut(s) 1038
BstSCI CCNGG 5 cut(s) 204, 553, 1332, 1337, 1338
BstSLI GKGCMC 1 cut(s) 466
BstUI CGCG 1 cut(s) 1266
BstV1I GCAGC 2 cut(s) 173, 1026
BstV2I GAAGAC 1 cut(s) 828
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
BstZ17I GTATAC 1 cut(s) 1148
Bsu36I CCTNAGG 1 cut(s) 27
BsuRI GGCC 4 cut(s) 563, 865, 1121, 1337
BtgI CCRYGG 1 cut(s) 564
BtsIMutI CAGTG 5 cut(s) 308, 409, 460, 984, 1056
BveI ACCTGC 2 cut(s) 1099, 1460
Cac8I GCNNGC 1 cut(s) 435
CfoI GCGC 1 cut(s) 233
Cfr10I RCCGGY 1 cut(s) 390
Cfr13I GGNCC 4 cut(s) 482, 561, 1329, 1336
Cfr9I CCCGGG 1 cut(s) 1338
CseI GACGC 1 cut(s) 1255
CsiI ACCWGGT 1 cut(s) 553
Csp6I GTAC 2 cut(s) 338, 413
CviAII CATG 8 cut(s) 61, 145, 234, 253, 565, 576, 1035, 1270
CviQI GTAC 2 cut(s) 338, 413
DdeI CTNAG 6 cut(s) 27, 795, 969, 1154, 1201, 1320
DpnI GATC 4 cut(s) 33, 247, 321, 959
DpnII GATC 4 cut(s) 31, 245, 319, 957
EciI GGCGGA 1 cut(s) 1320
Eco130I CCWWGG 3 cut(s) 151, 564, 1407
Eco31I GGTCTC 1 cut(s) 250
Eco47I GGWCC 2 cut(s) 482, 1329
Eco57I CTGAAG 4 cut(s) 201, 858, 1146, 1248
Eco81I CCTNAGG 1 cut(s) 27
Eco88I CYCGRG 1 cut(s) 1338
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRII CCWGG 3 cut(s) 204, 553, 1332
EcoT14I CCWWGG 3 cut(s) 151, 564, 1407
ErhI CCWWGG 3 cut(s) 151, 564, 1407
Esp3I CGTCTC 1 cut(s) 810
FaeI CATG 8 cut(s) 64, 148, 237, 256, 568, 579, 1038, 1273
FaqI GGGAC 3 cut(s) 107, 1306, 1342
FatI CATG 8 cut(s) 60, 144, 233, 252, 564, 575, 1034, 1269
FauNDI CATATG 1 cut(s) 316
FblI GTMKAC 2 cut(s) 352, 1147
Fnu4HI GCNGC 3 cut(s) 159, 162, 1015
Fsp4HI GCNGC 3 cut(s) 159, 162, 1015
FspBI CTAG 3 cut(s) 459, 1011, 1367
GlaI GCGC 1 cut(s) 232
GluI GCNGC 3 cut(s) 159, 162, 1015
GsaI CCCAGC 1 cut(s) 1374
GsuI CTGGAG 1 cut(s) 842
HaeIII GGCC 4 cut(s) 563, 865, 1121, 1337
HapII CCGG 2 cut(s) 391, 1339
HgaI GACGC 1 cut(s) 1255
HhaI GCGC 1 cut(s) 233
Hin1II CATG 8 cut(s) 64, 148, 237, 256, 568, 579, 1038, 1273
Hin6I GCGC 1 cut(s) 231
HinP1I GCGC 1 cut(s) 231
HincII GTYRAC 4 cut(s) 353, 388, 531, 1008
HindII GTYRAC 4 cut(s) 353, 388, 531, 1008
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 6 cut(s) 326, 656, 719, 845, 971, 976
HpaI GTTAAC 1 cut(s) 388
HpaII CCGG 2 cut(s) 391, 1339
HphI GGTGA 2 cut(s) 15, 1450
Hpy188I TCNGA 3 cut(s) 345, 470, 1126
Hpy188III TCNNGA 7 cut(s) 323, 517, 626, 666, 689, 859, 1396
HpyAV CCTTC 5 cut(s) 630, 663, 1217, 1232, 1425
HpyCH4III ACNGT 3 cut(s) 169, 827, 1051
HpyCH4V TGCA 9 cut(s) 4, 308, 377, 433, 464, 575, 1034, 1140, 1349
HpyF10VI GCNNNNNNNGC 2 cut(s) 1051, 1086
HpyF3I CTNAG 6 cut(s) 27, 795, 969, 1154, 1201, 1320
Hsp92II CATG 8 cut(s) 64, 148, 237, 256, 568, 579, 1038, 1273
HspAI GCGC 1 cut(s) 231
KflI GGGWCCC 1 cut(s) 1329
KspAI GTTAAC 1 cut(s) 388
Kzo9I GATC 4 cut(s) 31, 245, 319, 957
LmnI GCTCC 2 cut(s) 155, 1077
Lsp1109I GCAGC 2 cut(s) 173, 1026
LweI GCATC 1 cut(s) 823
MabI ACCWGGT 1 cut(s) 553
MaeI CTAG 3 cut(s) 459, 1011, 1367
MaeIII GTNAC 1 cut(s) 63
MalI GATC 4 cut(s) 33, 247, 321, 959
MbiI CCGCTC 1 cut(s) 383
MboI GATC 4 cut(s) 31, 245, 319, 957
MboII GAAGA 9 cut(s) 207, 275, 665, 821, 833, 851, 854, 1112, 1417
MfeI CAATTG 1 cut(s) 876
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 466
MluCI AATT 8 cut(s) 76, 117, 422, 511, 724, 760, 871, 876
MlyI GAGTC 2 cut(s) 980, 985
MmeI TCCRAC 1 cut(s) 813
MnlI CCTC 7 cut(s) 22, 726, 855, 1132, 1183, 1197, 1315
MseI TTAA 3 cut(s) 74, 387, 420
MspA1I CMGCKG 2 cut(s) 131, 1226
MspI CCGG 2 cut(s) 391, 1339
MspR9I CCNGG 5 cut(s) 206, 555, 1334, 1339, 1340
MunI CAATTG 1 cut(s) 876
Mva1269I GAATGC 1 cut(s) 435
MvaI CCWGG 3 cut(s) 206, 555, 1334
MvnI CGCG 1 cut(s) 1266
MwoI GCNNNNNNNGC 2 cut(s) 1051, 1086
NciI CCSGG 2 cut(s) 1339, 1340
NcoI CCATGG 1 cut(s) 564
NdeI CATATG 1 cut(s) 316
NdeII GATC 4 cut(s) 31, 245, 319, 957
NlaIII CATG 8 cut(s) 64, 148, 237, 256, 568, 579, 1038, 1273
NlaIV GGNNCC 5 cut(s) 33, 157, 1330, 1331, 1413
NmuCI GTSAC 1 cut(s) 63
NspI RCATGY 1 cut(s) 1038
PaqCI CACCTGC 1 cut(s) 1460
PctI GAATGC 1 cut(s) 435
PfeI GAWTC 4 cut(s) 326, 656, 719, 845
PkrI GCNGC 3 cut(s) 160, 163, 1016
PleI GAGTC 2 cut(s) 979, 984
PpsI GAGTC 2 cut(s) 979, 984
PpuMI RGGWCCY 1 cut(s) 1329
PsiI TTATAA 1 cut(s) 740
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 3 cut(s) 204, 553, 1332
PspFI CCCAGC 1 cut(s) 1370
PspGI CCWGG 3 cut(s) 204, 553, 1332
PspN4I GGNNCC 5 cut(s) 33, 157, 1330, 1331, 1413
PspPI GGNCC 4 cut(s) 482, 561, 1329, 1336
PspPPI RGGWCCY 1 cut(s) 1329
PsuI RGATCY 1 cut(s) 31
PvuII CAGCTG 2 cut(s) 131, 1226
RsaI GTAC 2 cut(s) 339, 414
RsaNI GTAC 2 cut(s) 338, 413
SalI GTCGAC 1 cut(s) 351
SaqAI TTAA 3 cut(s) 74, 387, 420
SatI GCNGC 3 cut(s) 159, 162, 1015
Sau3AI GATC 4 cut(s) 31, 245, 319, 957
Sau96I GGNCC 4 cut(s) 482, 561, 1329, 1336
ScaI AGTACT 1 cut(s) 339
SchI GAGTC 2 cut(s) 980, 985
ScrFI CCNGG 5 cut(s) 206, 555, 1334, 1339, 1340
SduI GDGCHC 1 cut(s) 466
SexAI ACCWGGT 1 cut(s) 553
SfaNI GCATC 1 cut(s) 823
SinI GGWCC 2 cut(s) 482, 1329
SmaI CCCGGG 1 cut(s) 1340
Sse9I AATT 8 cut(s) 76, 117, 422, 511, 724, 760, 871, 876
SsiI CCGC 3 cut(s) 159, 383, 1305
SspI AATATT 2 cut(s) 745, 1026
SspMI CTAG 3 cut(s) 459, 1011, 1367
StyD4I CCNGG 5 cut(s) 204, 553, 1332, 1337, 1338
StyI CCWWGG 3 cut(s) 151, 564, 1407
TaaI ACNGT 3 cut(s) 169, 827, 1051
TaqI TCGA 2 cut(s) 352, 974
TasI AATT 8 cut(s) 76, 117, 422, 511, 724, 760, 871, 876
TatI WGTACW 1 cut(s) 337
TauI GCSGC 1 cut(s) 161
TfiI GAWTC 4 cut(s) 326, 656, 719, 845
Tru1I TTAA 3 cut(s) 74, 387, 420
Tru9I TTAA 3 cut(s) 74, 387, 420
TscAI CASTG 5 cut(s) 315, 409, 460, 991, 1056
TseFI GTSAC 1 cut(s) 63
TseI GCWGC 2 cut(s) 161, 1014
Tsp45I GTSAC 1 cut(s) 63
TspDTI ATGAA 5 cut(s) 49, 318, 555, 797, 822
TspMI CCCGGG 1 cut(s) 1338
TspRI CASTG 5 cut(s) 315, 409, 460, 991, 1056
VneI GTGCAC 1 cut(s) 462
VpaK11BI GGWCC 2 cut(s) 482, 1329
XapI RAATTY 1 cut(s) 76
XceI RCATGY 1 cut(s) 1038
XmaI CCCGGG 1 cut(s) 1338
XmiI GTMKAC 2 cut(s) 352, 1147
XspI CTAG 3 cut(s) 459, 1011, 1367
ZrmI AGTACT 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.