AT1G04490

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0004960
Physical Location & Seq
Reverse (-)
1217690 .. 1220899
3210 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04490.3

Sequence Viewer

Length: 1206 bp
ATGGAAGGTCAAACTCATGATGCTCAAAACAATGTTCCTGAGCATGACAATCCATTGATGAATGTGAAGAATCCATCAAGCAACTTGTTTTCTAAGAAAGATGCACAATGTTTGTCTGTTTTATGCAACGGGAAATGGTTACAGATTGTTAGAAGCAGTCCCAAAGTATCATGTGAGGCCTCTACTTGTCCAAGTCATATTTTGACCAAAGTAGGAGAAAACTATGTGGCTGAATCTGAAAAGATGCATAAAAAGGTAATCTCTTTCTCGAGCAAAACCTTCTCTCAGGGAACCCTTCAATTCACTATGAGAGCCAATGGAACACCTCATTTTGTTTTCAAATTGGAGAATCAAAAAGATGTTTATGTGGCAAGCTTAAGCAGCAATGTCCAAGACCAAAACTCGTATATGATCCATCTGCAAAGAGGAGAATCATCAGCATCCTCATCACATCTAGTTGGGAGAATAAATGTATCTACTTTGTTCTCGGAGAAAGTCTTGGAGAGAGAGTTTGTTTTGTTCAGTAGCAATGGTGAGAATCTCAAGATACCGCGTACGAGGAAAAACAGAGGACTGTCTAAAAAAGTAGTCCATGCGGTCAAGAACGAAAGAAGAACAGCAAGATTGAGCAGAACAAGCTTCATACCTGATTTAGGTTCTTGGGATGAGCAATTCCAAGCACAAAACTATGATTGTCTGCTCAAAAACAAGCTTCCAACAAATCTTGAAACATTAGCTGTTGTTGTGAAACAAGAGACTATTGAGGACGAAATTGGAGGATGGGGACTAAAGTTCTTGAAGAGATCTCCCATGTTCCAAAGAAGTAATGATGCTAGTGAGACTGAAACTTCAACATCTTCAATAAGTATGAATGTTGTGATTCCTTCAGGGATTCATGGAGGGCCTGAAGATGGACCTTCGAGTTTGATAGAGAGATGGAAATCTCAAGGAAATTGTGACTGTGGAGGATGGGATTTGTGTTGTTCCTTGACCCTTCTTAAAGGCCAACCACGGAAAGACCAGTACTTCGAACTATTCATAGAGGGGTCAAAACATGAAACAACAGGACTAAAGATTGTGAATGTTAGTGGAGGACTCTACTTAGTTCAATTCGAAGCAAAGTTGACTAGTCTGCAATCATTTGCAATTGCTTTGGCATTCATACATAGCGAGAAGCTTAGACCGTTGCATCTATACGTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

401

Amino Acids

44.83

Weight (kDa)

8.73

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 93 - 390 2.2e-76 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 553
AciI CCGC 2 cut(s) 551, 596
AclWI GGATC 1 cut(s) 406
AcuI CTGAAG 2 cut(s) 870, 927
AfaI GTAC 2 cut(s) 556, 1025
AfiI CCNNNNNNNGG 2 cut(s) 653, 911
AflII CTTAAG 1 cut(s) 376
AgsI TTSAA 7 cut(s) 299, 340, 728, 799, 852, 861, 1109
AhlI ACTAGT 1 cut(s) 1127
AluBI AGCT 5 cut(s) 375, 639, 712, 737, 1177
AluI AGCT 5 cut(s) 375, 639, 712, 737, 1177
Alw26I GTCTC 2 cut(s) 749, 833
AlwI GGATC 1 cut(s) 406
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 3 cut(s) 177, 902, 1003
ApeKI GCWGC 1 cut(s) 381
ArsI GACNNNNNNTTYG 2 cut(s) 1010, 1042
AspS9I GGNCC 2 cut(s) 902, 914
AsuHPI GGTGA 1 cut(s) 545
AsuII TTCGAA 2 cut(s) 1029, 1113
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 1 cut(s) 914
BbvI GCAGC 1 cut(s) 393
BccI CCATC 6 cut(s) 82, 423, 774, 905, 930, 963
BcoDI GTCTC 2 cut(s) 749, 833
BcuI ACTAGT 1 cut(s) 1127
BfaI CTAG 3 cut(s) 455, 834, 1128
BfrI CTTAAG 1 cut(s) 376
BglII AGATCT 1 cut(s) 803
BisI GCNGC 1 cut(s) 382
BlsI GCNGC 1 cut(s) 383
BmcAI AGTACT 1 cut(s) 1025
Bme18I GGWCC 1 cut(s) 914
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 2 cut(s) 902, 914
BmiI GGNNCC 1 cut(s) 292
BmsI GCATC 6 cut(s) 10, 91, 234, 449, 820, 1198
Bpu10I CCTNAGC 1 cut(s) 39
Bpu14I TTCGAA 2 cut(s) 1029, 1113
BpuEI CTTGAG 2 cut(s) 527, 930
BsaAI YACGTR 1 cut(s) 1198
BsaBI GATNNNNATC 1 cut(s) 940
BsaJI CCNNGG 1 cut(s) 1010
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 653, 911
Bse1I ACTGG 1 cut(s) 1021
Bse3DI GCAATG 2 cut(s) 391, 535
Bse8I GATNNNNATC 1 cut(s) 940
BseDI CCNNGG 1 cut(s) 1010
BseGI GGATG 4 cut(s) 440, 670, 785, 974
BseJI GATNNNNATC 1 cut(s) 940
BseLI CCNNNNNNNGG 2 cut(s) 653, 911
BseMI GCAATG 2 cut(s) 391, 535
BseMII CTCAG 2 cut(s) 30, 299
BseNI ACTGG 1 cut(s) 1021
BseRI GAGGAG 1 cut(s) 441
BseXI GCAGC 1 cut(s) 393
Bsh1236I CGCG 1 cut(s) 553
BshFI GGCC 3 cut(s) 179, 904, 1005
BsiHKCI CYCGRG 1 cut(s) 268
BsiWI CGTACG 1 cut(s) 554
BslFI GGGAC 2 cut(s) 144, 798
BslI CCNNNNNNNGG 2 cut(s) 653, 911
BsmAI GTCTC 2 cut(s) 749, 833
BsmFI GGGAC 2 cut(s) 144, 798
BsmI GAATGC 1 cut(s) 1157
BsnI GGCC 3 cut(s) 179, 904, 1005
BsoBI CYCGRG 1 cut(s) 268
Bsp119I TTCGAA 2 cut(s) 1029, 1113
Bsp143I GATC 2 cut(s) 411, 803
BspACI CCGC 2 cut(s) 551, 596
BspANI GGCC 3 cut(s) 179, 904, 1005
BspCNI CTCAG 2 cut(s) 31, 298
BspFNI CGCG 1 cut(s) 553
BspHI TCATGA 1 cut(s) 16
BspLI GGNNCC 1 cut(s) 292
BspPI GGATC 1 cut(s) 406
BspT104I TTCGAA 2 cut(s) 1029, 1113
BspTI CTTAAG 1 cut(s) 376
BsrDI GCAATG 2 cut(s) 391, 535
BsrI ACTGG 1 cut(s) 1021
BssECI CCNNGG 1 cut(s) 1010
BssMI GATC 2 cut(s) 411, 803
Bst4CI ACNGT 3 cut(s) 576, 962, 1185
Bst6I CTCTTC 1 cut(s) 794
BstAFI CTTAAG 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 1198
BstBI TTCGAA 2 cut(s) 1029, 1113
BstC8I GCNNGC 1 cut(s) 373
BstDEI CTNAG 5 cut(s) 39, 93, 285, 1102, 1178
BstDSI CCRYGG 1 cut(s) 1010
BstENI CCTNNNNNAGG 1 cut(s) 651
BstF5I GGATG 4 cut(s) 440, 670, 785, 974
BstFNI CGCG 1 cut(s) 553
BstKTI GATC 2 cut(s) 414, 806
BstMAI GTCTC 2 cut(s) 749, 833
BstMBI GATC 2 cut(s) 411, 803
BstMWI GCNNNNNNNGC 2 cut(s) 381, 636
BstSNI TACGTA 1 cut(s) 1198
BstUI CGCG 1 cut(s) 553
BstV1I GCAGC 1 cut(s) 393
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 3 cut(s) 179, 904, 1005
BtgI CCRYGG 1 cut(s) 1010
BtsCI GGATG 4 cut(s) 440, 670, 785, 974
Cac8I GCNNGC 1 cut(s) 373
CciI TCATGA 1 cut(s) 16
Cfr13I GGNCC 2 cut(s) 902, 914
Csp6I GTAC 2 cut(s) 555, 1024
CviAII CATG 7 cut(s) 17, 44, 171, 593, 811, 896, 1055
CviQI GTAC 2 cut(s) 555, 1024
DdeI CTNAG 5 cut(s) 39, 93, 285, 1102, 1178
DpnI GATC 2 cut(s) 413, 805
DpnII GATC 2 cut(s) 411, 803
Eam1104I CTCTTC 1 cut(s) 794
EarI CTCTTC 1 cut(s) 794
Eco105I TACGTA 1 cut(s) 1198
Eco147I AGGCCT 1 cut(s) 179
Eco47I GGWCC 1 cut(s) 914
Eco57I CTGAAG 2 cut(s) 870, 927
Eco88I CYCGRG 1 cut(s) 268
EcoNI CCTNNNNNAGG 1 cut(s) 651
EcoO109I RGGNCCY 1 cut(s) 902
EcoT22I ATGCAT 1 cut(s) 249
FaeI CATG 7 cut(s) 20, 47, 174, 596, 814, 899, 1058
FaqI GGGAC 2 cut(s) 144, 798
FatI CATG 7 cut(s) 16, 43, 170, 592, 810, 895, 1054
Fnu4HI GCNGC 1 cut(s) 382
FokI GGATG 4 cut(s) 427, 677, 792, 981
Fsp4HI GCNGC 1 cut(s) 382
FspBI CTAG 3 cut(s) 455, 834, 1128
GluI GCNGC 1 cut(s) 382
HaeIII GGCC 3 cut(s) 179, 904, 1005
Hin1II CATG 7 cut(s) 20, 47, 174, 596, 814, 899, 1058
HincII GTYRAC 1 cut(s) 1125
HindII GTYRAC 1 cut(s) 1125
HindIII AAGCTT 4 cut(s) 373, 637, 710, 1175
HinfI GANTC 8 cut(s) 70, 233, 349, 431, 538, 880, 892, 1095
HphI GGTGA 1 cut(s) 545
Hpy166II GTNNAC 1 cut(s) 1125
Hpy188I TCNGA 2 cut(s) 238, 490
Hpy188III TCNNGA 7 cut(s) 17, 38, 268, 544, 601, 725, 796
Hpy8I GTNNAC 1 cut(s) 1125
HpyAV CCTTC 5 cut(s) 289, 305, 894, 927, 1004
HpyCH4III ACNGT 3 cut(s) 576, 962, 1185
HpyCH4IV ACGT 1 cut(s) 1197
HpyCH4V TGCA 7 cut(s) 104, 126, 247, 421, 1135, 1145, 1189
HpyF10VI GCNNNNNNNGC 2 cut(s) 381, 636
HpyF3I CTNAG 5 cut(s) 39, 93, 285, 1102, 1178
HpySE526I ACGT 1 cut(s) 1197
Hsp92II CATG 7 cut(s) 20, 47, 174, 596, 814, 899, 1058
Kzo9I GATC 2 cut(s) 411, 803
LpnPI CCDG 7 cut(s) 51, 272, 660, 873, 918, 1034, 1050
Lsp1109I GCAGC 1 cut(s) 393
LweI GCATC 6 cut(s) 10, 91, 234, 449, 820, 1198
MaeI CTAG 3 cut(s) 455, 834, 1128
MaeII ACGT 1 cut(s) 1197
MaeIII GTNAC 2 cut(s) 138, 956
MalI GATC 2 cut(s) 413, 805
MboI GATC 2 cut(s) 411, 803
MboII GAAGA 5 cut(s) 79, 624, 811, 849, 920
MfeI CAATTG 1 cut(s) 1146
MflI RGATCY 1 cut(s) 803
MluCI AATT 7 cut(s) 299, 341, 671, 771, 952, 1109, 1146
MlyI GAGTC 1 cut(s) 1089
MmeI TCCRAC 1 cut(s) 740
Mph1103I ATGCAT 1 cut(s) 249
MseI TTAA 3 cut(s) 377, 999, 1204
MspCI CTTAAG 1 cut(s) 376
MunI CAATTG 1 cut(s) 1146
Mva1269I GAATGC 1 cut(s) 1157
MvnI CGCG 1 cut(s) 553
MwoI GCNNNNNNNGC 2 cut(s) 381, 636
NdeII GATC 2 cut(s) 411, 803
NlaIII CATG 7 cut(s) 20, 47, 174, 596, 814, 899, 1058
NlaIV GGNNCC 1 cut(s) 292
NmuCI GTSAC 1 cut(s) 956
NsiI ATGCAT 1 cut(s) 249
NspV TTCGAA 2 cut(s) 1029, 1113
PaeR7I CTCGAG 1 cut(s) 268
PagI TCATGA 1 cut(s) 16
PceI AGGCCT 1 cut(s) 179
PctI GAATGC 1 cut(s) 1157
PfeI GAWTC 7 cut(s) 70, 233, 349, 431, 538, 880, 892
Pfl23II CGTACG 1 cut(s) 554
PkrI GCNGC 1 cut(s) 383
PleI GAGTC 1 cut(s) 1089
PpsI GAGTC 1 cut(s) 1089
Ppu21I YACGTR 1 cut(s) 1198
PspLI CGTACG 1 cut(s) 554
PspN4I GGNNCC 1 cut(s) 292
PspPI GGNCC 2 cut(s) 902, 914
PsuI RGATCY 1 cut(s) 803
RsaI GTAC 2 cut(s) 556, 1025
RsaNI GTAC 2 cut(s) 555, 1024
SaqAI TTAA 3 cut(s) 377, 999, 1204
SatI GCNGC 1 cut(s) 382
Sau3AI GATC 2 cut(s) 411, 803
Sau96I GGNCC 2 cut(s) 902, 914
ScaI AGTACT 1 cut(s) 1025
SchI GAGTC 1 cut(s) 1089
SfaNI GCATC 6 cut(s) 10, 91, 234, 449, 820, 1198
Sfr274I CTCGAG 1 cut(s) 268
SfuI TTCGAA 2 cut(s) 1029, 1113
SinI GGWCC 1 cut(s) 914
SlaI CTCGAG 1 cut(s) 268
SmlI CTYRAG 4 cut(s) 268, 376, 542, 945
SmoI CTYRAG 4 cut(s) 268, 376, 542, 945
SnaBI TACGTA 1 cut(s) 1198
SpeI ACTAGT 1 cut(s) 1127
Sse9I AATT 7 cut(s) 299, 341, 671, 771, 952, 1109, 1146
SseBI AGGCCT 1 cut(s) 179
SsiI CCGC 2 cut(s) 551, 596
SspMI CTAG 3 cut(s) 455, 834, 1128
StuI AGGCCT 1 cut(s) 179
TaaI ACNGT 3 cut(s) 576, 962, 1185
TaiI ACGT 1 cut(s) 1200
TaqI TCGA 4 cut(s) 269, 920, 1029, 1113
TasI AATT 7 cut(s) 299, 341, 671, 771, 952, 1109, 1146
TatI WGTACW 1 cut(s) 1023
TfiI GAWTC 7 cut(s) 70, 233, 349, 431, 538, 880, 892
Tru1I TTAA 3 cut(s) 377, 999, 1204
Tru9I TTAA 3 cut(s) 377, 999, 1204
TseFI GTSAC 1 cut(s) 956
TseI GCWGC 1 cut(s) 381
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 7 cut(s) 74, 631, 884, 884, 1027, 1071, 1150
TspGWI ACGGA 1 cut(s) 1027
Vha464I CTTAAG 1 cut(s) 376
VpaK11BI GGWCC 1 cut(s) 914
XagI CCTNNNNNAGG 1 cut(s) 651
XhoI CTCGAG 1 cut(s) 268
XspI CTAG 3 cut(s) 455, 834, 1128
ZrmI AGTACT 1 cut(s) 1025
Zsp2I ATGCAT 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.