AT2G33360

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0004960
Physical Location & Seq
Forward (+)
14139760 .. 14143118
3359 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33360.1

Sequence Viewer

Length: 1812 bp
ATGGGAAGTGTTTCTCCTGATGCTCAGAAGGATGATCTTGAGCTTGAAGATTCATTGAACATTGATGAGAATCTGTCTGGGGGAAATGGCTTATTGAAGTCAGGGAACTTGTTGAGCCAGAAAAGACAGCAATGTAGTTTAGTTTCAAACAGCGGAAAGTGGTTACAGATTGTTAAAAACGGTCCCAAGGGATCTTTTTCTCGTGCCACTAGTTTCTTCGATAACAAGATCCCAAGGCAGTTGGTGTCAGTAGACGAGAAGTATCTCCGTCGATGCCTTGACCTGATTAATATCAGTGCGTTTAAGTCAGCCAGTTGCAGCCTTTCTTTGAATCTTGTAGGATCAAAGATGAGTTTGTCTTCACGTCGCTTCGATTTACCTGTTATCCCCAAAGAAAATGTTGCTCGCCTTGTGTTTGATCTTCCGTTGGTAGATGATTCAGGCAGTCCAGTGATTAGCCGTGCAATTATAGGTTGCAAGAGGGTTACATATATGCTTGATAAGCCTACGTTACACAGTCTCGAAGCATTAGATGGTGATGGTAATAATATCGGGACAAGGCGAGACCATAATGGTGAAAGCGAGTTGGTTTCATATGATGTTGTGAGGAAGATAGAGGAAAAAAACACCCCGGATTCACAGTTTCTGAGTTATCATTCTGGAAACATGCCCCGAAAGGCAGTTTCTTTATCAAGCACAAATTCATTGAGTTCTTCTTCTTCTGAGCAATCTTCTTCCTCGTGGTCTCCATCTTCATCAGTTTCTCAAGGAACACTTCAATTCACTATGAAAGACAACAAGACGCCTCATTTTGTTTTCTCACTGGATGATCAGAAAGAGATCTATGTAGCTAGCTTAAGCACTACTAGTGTTGGGTCAGGTTTCGACCGTAGTTCTCTAGACTATTCGTATTTGATCCATTTGAAGAAAGGTCGCGGATCTGAGCCGCAGCATTTGGTTGGTAAACTAAAGGTATCAACTTTGTTTTCTGTCTCGTCCACGAATGAAAAAACTGTAGAGAGACAGTTTGTTCTATTCAGCAGTGGTGGAAATCCGCAGTTACCGTGCCACAAAGATATTAGGAAAAACAGAGGATTGCCTAAGAAAGTCGTTGATGCTCTCAAAAGCACCAAACGCACTTCAAGGCAAAGAAGTATCTCAAGATTCAGTAGAACAAGCTCCATACCGGATTTTTGTTCTTGGGAGCCATTTCAAGAACATGACAGCGATCTTGAACCCATAAGTTTACTGGATAATGACCTTCCACCAAATCTTGAAACCTCAGCTGTTGTTGTGAGAGAACAGTTTCCAGTGGAAGAAGAAGAAGAAGAAGAAGTCGAAAAAGTTGGAGGCTGGGGCATGAAGTTTCTCAAGAAAATTCCCTTAGCTCGAACCAAGGATGCTTCTAAATGTTCTAAGCATTCCACAAGCATAGATGTTGTGATTCCTTTAGGGATTCACGGAGGGCCGAGAAATAGAAACGGTGGTCCTTCGAGCTTGATTCAGAGATGGAAATCCGGTGGATGCTGTGATTGCTCTGGATGGGACTTGGGTTGCCCCTTAACCGTCCTTAAAGGCCAAGCGCGAAAAGACCAAAGTGAAGGCCAATGCAATCTTTTTGAGCTTTTCACAGAGGGATTGATGCAGGGAAGTCCTGGACTAAGGATCATGAATGTTCGAGATGGTCTCTACTTTGTCCAGTCTCAAGCAAAGATATCAGTTTTGCAATCTTTTTCAATTGCTTTAGCATACATTCATAGCCAAAGCCAGAGACTCCGGCCATGGTCGTCAAAGAAGTGTACAGAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

603

Amino Acids

66.51

Weight (kDa)

8.79

Isoelectric Point (pI)

54.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 252 - 588 1.2e-113 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 252
AccII CGCG 2 cut(s) 936, 1585
AciI CCGC 4 cut(s) 153, 936, 947, 1055
AclWI GGATC 6 cut(s) 199, 223, 349, 910, 946, 1673
AcoI YGGCCR 1 cut(s) 1778
AcsI RAATTY 2 cut(s) 700, 1377
AcyI GRCGYC 1 cut(s) 803
AfaI GTAC 1 cut(s) 1801
AflII CTTAAG 1 cut(s) 856
AhlI ACTAGT 2 cut(s) 209, 866
AjiI CACGTC 1 cut(s) 365
AjnI CCWGG 1 cut(s) 1654
AluBI AGCT 9 cut(s) 43, 851, 855, 1179, 1286, 1388, 1497, 1624, 1808
AluI AGCT 9 cut(s) 43, 851, 855, 1179, 1286, 1388, 1497, 1624, 1808
Alw26I GTCTC 8 cut(s) 524, 558, 750, 997, 1015, 1691, 1707, 1765
AlwI GGATC 6 cut(s) 199, 223, 349, 910, 946, 1673
AlwNI CAGNNNCTG 1 cut(s) 646
AoxI GGCC 4 cut(s) 1466, 1576, 1603, 1778
ApeKI GCWGC 2 cut(s) 318, 949
ApoI RAATTY 2 cut(s) 700, 1377
AseI ATTAAT 1 cut(s) 288
Asp700I GAANNNNTTC 2 cut(s) 10, 1305
AspLEI GCGC 1 cut(s) 1585
AspS9I GGNCC 3 cut(s) 182, 1466, 1487
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 2 cut(s) 548, 587
AsuNHI GCTAGC 1 cut(s) 851
AvaII GGWCC 2 cut(s) 182, 1487
BauI CACGAG 2 cut(s) 201, 739
BbsI GAAGAC 1 cut(s) 351
BbvCI CCTCAGC 1 cut(s) 1282
BbvI GCAGC 2 cut(s) 330, 961
BccI CCATC 6 cut(s) 527, 533, 757, 1503, 1536, 1676
BceAI ACGGC 1 cut(s) 444
BciT130I CCWGG 1 cut(s) 1656
BclI TGATCA 1 cut(s) 829
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 8 cut(s) 524, 558, 750, 997, 1015, 1691, 1707, 1765
BcuI ACTAGT 2 cut(s) 209, 866
BfaI CTAG 4 cut(s) 210, 852, 867, 899
BfmI CTRYAG 1 cut(s) 1014
BfrI CTTAAG 1 cut(s) 856
BglII AGATCT 1 cut(s) 840
BisI GCNGC 3 cut(s) 319, 947, 950
BlsI GCNGC 3 cut(s) 320, 948, 951
Bme1390I CCNGG 2 cut(s) 632, 1656
Bme18I GGWCC 2 cut(s) 182, 1487
BmgBI CACGTC 1 cut(s) 365
BmgT120I GGNCC 3 cut(s) 182, 1466, 1487
BmiI GGNNCC 2 cut(s) 184, 1206
BmrFI CCNGG 2 cut(s) 632, 1656
BmsI GCATC 6 cut(s) 10, 263, 1105, 1390, 1514, 1632
BmtI GCTAGC 1 cut(s) 855
BpiI GAAGAC 1 cut(s) 351
BplI GAGNNNNNCTC 2 cut(s) 1671, 1703
Bpu10I CCTNAGC 2 cut(s) 1282, 1384
BpuEI CTTGAG 5 cut(s) 59, 750, 1144, 1355, 1689
BpuMI CCSGG 1 cut(s) 632
BsaBI GATNNNNATC 3 cut(s) 69, 290, 1513
BsaHI GRCGYC 1 cut(s) 803
BsaI GGTCTC 3 cut(s) 558, 750, 1691
BsaJI CCNNGG 5 cut(s) 186, 233, 630, 1395, 1781
BsaWI WCCGGW 2 cut(s) 1186, 1517
Bse1I ACTGG 6 cut(s) 312, 449, 828, 1254, 1310, 1699
Bse3DI GCAATG 1 cut(s) 137
Bse8I GATNNNNATC 3 cut(s) 69, 290, 1513
BseBI CCWGG 1 cut(s) 1656
BseDI CCNNGG 5 cut(s) 186, 233, 630, 1395, 1781
BseGI GGATG 5 cut(s) 37, 832, 1405, 1529, 1547
BseJI GATNNNNATC 3 cut(s) 69, 290, 1513
BseMI GCAATG 1 cut(s) 137
BseMII CTCAG 5 cut(s) 38, 638, 714, 933, 1296
BseNI ACTGG 6 cut(s) 312, 449, 828, 1254, 1310, 1699
BseXI GCAGC 2 cut(s) 330, 961
BseYI CCCAGC 1 cut(s) 1353
Bsh1236I CGCG 2 cut(s) 936, 1585
Bsh1285I CGRYCG 1 cut(s) 889
BshFI GGCC 4 cut(s) 1468, 1578, 1605, 1780
BsiEI CGRYCG 1 cut(s) 889
BsiSI CCGG 4 cut(s) 632, 1187, 1518, 1777
BslFI GGGAC 3 cut(s) 168, 568, 1559
BsmAI GTCTC 8 cut(s) 524, 558, 750, 997, 1015, 1691, 1707, 1765
BsmFI GGGAC 3 cut(s) 168, 568, 1559
BsmI GAATGC 1 cut(s) 1420
BsnI GGCC 4 cut(s) 1468, 1578, 1605, 1780
Bso31I GGTCTC 3 cut(s) 558, 750, 1691
Bsp1407I TGTACA 1 cut(s) 1799
Bsp19I CCATGG 1 cut(s) 1781
BspACI CCGC 4 cut(s) 153, 936, 947, 1055
BspANI GGCC 4 cut(s) 1468, 1578, 1605, 1780
BspCNI CTCAG 5 cut(s) 37, 639, 715, 934, 1295
BspFNI CGCG 2 cut(s) 936, 1585
BspHI TCATGA 1 cut(s) 1668
BspLI GGNNCC 2 cut(s) 184, 1206
BspOI GCTAGC 1 cut(s) 855
BspPI GGATC 6 cut(s) 199, 223, 349, 910, 946, 1673
BspTI CTTAAG 1 cut(s) 856
BspTNI GGTCTC 3 cut(s) 558, 750, 1691
BsrDI GCAATG 1 cut(s) 137
BsrGI TGTACA 1 cut(s) 1799
BsrI ACTGG 6 cut(s) 312, 449, 828, 1254, 1310, 1699
BssECI CCNNGG 5 cut(s) 186, 233, 630, 1395, 1781
BssNI GRCGYC 1 cut(s) 803
BssSI CACGAG 2 cut(s) 201, 739
BssT1I CCWWGG 4 cut(s) 186, 233, 1395, 1781
Bst2BI CACGAG 2 cut(s) 201, 739
Bst2UI CCWGG 1 cut(s) 1656
BstACI GRCGYC 1 cut(s) 803
BstAFI CTTAAG 1 cut(s) 856
BstAUI TGTACA 1 cut(s) 1799
BstC8I GCNNGC 2 cut(s) 406, 853
BstDEI CTNAG 9 cut(s) 24, 647, 723, 942, 1101, 1282, 1384, 1416, 1661
BstDSI CCRYGG 1 cut(s) 1781
BstF5I GGATG 5 cut(s) 37, 832, 1405, 1529, 1547
BstFNI CGCG 2 cut(s) 936, 1585
BstHHI GCGC 1 cut(s) 1585
BstMAI GTCTC 8 cut(s) 524, 558, 750, 997, 1015, 1691, 1707, 1765
BstMCI CGRYCG 1 cut(s) 889
BstMWI GCNNNNNNNGC 3 cut(s) 502, 1134, 1533
BstNI CCWGG 1 cut(s) 1656
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 630, 1654
BstSFI CTRYAG 1 cut(s) 1014
BstUI CGCG 2 cut(s) 936, 1585
BstV1I GCAGC 2 cut(s) 330, 961
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 4 cut(s) 191, 228, 840, 938
BstYI RGATCY 4 cut(s) 191, 228, 840, 938
BsuRI GGCC 4 cut(s) 1468, 1578, 1605, 1780
BtgI CCRYGG 1 cut(s) 1781
BtrI CACGTC 1 cut(s) 365
BtsCI GGATG 5 cut(s) 37, 832, 1405, 1529, 1547
BtsI GCAGTG 1 cut(s) 1048
BtsIMutI CAGTG 5 cut(s) 301, 456, 821, 1048, 1317
Cac8I GCNNGC 2 cut(s) 406, 853
CaiI CAGNNNCTG 1 cut(s) 646
CciI TCATGA 1 cut(s) 1668
CfoI GCGC 1 cut(s) 1585
Cfr13I GGNCC 3 cut(s) 182, 1466, 1487
CseI GACGC 1 cut(s) 811
Csp6I GTAC 1 cut(s) 1800
CviAII CATG 5 cut(s) 667, 1220, 1360, 1669, 1782
CviQI GTAC 1 cut(s) 1800
DdeI CTNAG 9 cut(s) 24, 647, 723, 942, 1101, 1282, 1384, 1416, 1661
EaeI YGGCCR 1 cut(s) 1778
Eco130I CCWWGG 4 cut(s) 186, 233, 1395, 1781
Eco31I GGTCTC 3 cut(s) 558, 750, 1691
Eco32I GATATC 1 cut(s) 1716
Eco47I GGWCC 2 cut(s) 182, 1487
EcoRII CCWGG 1 cut(s) 1654
EcoRV GATATC 1 cut(s) 1716
EcoT14I CCWWGG 4 cut(s) 186, 233, 1395, 1781
ErhI CCWWGG 4 cut(s) 186, 233, 1395, 1781
FaeI CATG 5 cut(s) 670, 1223, 1363, 1672, 1785
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FaqI GGGAC 3 cut(s) 168, 568, 1559
FatI CATG 5 cut(s) 666, 1219, 1359, 1668, 1781
FauNDI CATATG 1 cut(s) 595
FbaI TGATCA 1 cut(s) 829
FblI GTMKAC 1 cut(s) 252
Fnu4HI GCNGC 3 cut(s) 319, 947, 950
FokI GGATG 5 cut(s) 44, 839, 1412, 1536, 1554
Fsp4HI GCNGC 3 cut(s) 319, 947, 950
FspBI CTAG 4 cut(s) 210, 852, 867, 899
GlaI GCGC 1 cut(s) 1584
GluI GCNGC 3 cut(s) 319, 947, 950
GsaI CCCAGC 1 cut(s) 1357
HaeIII GGCC 4 cut(s) 1468, 1578, 1605, 1780
HapII CCGG 4 cut(s) 632, 1187, 1518, 1777
HgaI GACGC 1 cut(s) 811
HhaI GCGC 1 cut(s) 1585
Hin1I GRCGYC 1 cut(s) 803
Hin1II CATG 5 cut(s) 670, 1223, 1363, 1672, 1785
Hin6I GCGC 1 cut(s) 1583
HinP1I GCGC 1 cut(s) 1583
HindIII AAGCTT 1 cut(s) 1806
HpaII CCGG 4 cut(s) 632, 1187, 1518, 1777
HphI GGTGA 2 cut(s) 548, 587
Hpy166II GTNNAC 5 cut(s) 253, 965, 999, 1247, 1800
Hpy188I TCNGA 6 cut(s) 27, 648, 724, 834, 943, 1506
Hpy8I GTNNAC 5 cut(s) 253, 965, 999, 1247, 1800
Hpy99I CGWCG 2 cut(s) 273, 369
HpyAV CCTTC 4 cut(s) 22, 1271, 1500, 1595
HpyCH4IV ACGT 2 cut(s) 364, 509
HpyCH4V TGCA 6 cut(s) 318, 464, 477, 1611, 1645, 1726
HpyF10VI GCNNNNNNNGC 3 cut(s) 502, 1134, 1533
HpyF3I CTNAG 9 cut(s) 24, 647, 723, 942, 1101, 1282, 1384, 1416, 1661
HpySE526I ACGT 2 cut(s) 364, 509
Hsp92I GRCGYC 1 cut(s) 803
Hsp92II CATG 5 cut(s) 670, 1223, 1363, 1672, 1785
HspAI GCGC 1 cut(s) 1583
Ksp22I TGATCA 1 cut(s) 829
LmnI GCTCC 2 cut(s) 1184, 1204
Lsp1109I GCAGC 2 cut(s) 330, 961
LweI GCATC 6 cut(s) 10, 263, 1105, 1390, 1514, 1632
MaeI CTAG 4 cut(s) 210, 852, 867, 899
MaeII ACGT 2 cut(s) 364, 509
MaeIII GTNAC 4 cut(s) 162, 484, 510, 1059
MfeI CAATTG 1 cut(s) 1737
MflI RGATCY 4 cut(s) 191, 228, 840, 938
MluCI AATT 5 cut(s) 465, 700, 779, 1377, 1737
MlyI GAGTC 1 cut(s) 1767
MmeI TCCRAC 1 cut(s) 1327
MroXI GAANNNNTTC 2 cut(s) 10, 1305
MseI TTAA 7 cut(s) 174, 288, 303, 857, 1562, 1572, 1810
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 2 cut(s) 153, 1286
MspCI CTTAAG 1 cut(s) 856
MspI CCGG 4 cut(s) 632, 1187, 1518, 1777
MspR9I CCNGG 2 cut(s) 632, 1656
MunI CAATTG 1 cut(s) 1737
Mva1269I GAATGC 1 cut(s) 1420
MvaI CCWGG 1 cut(s) 1656
MvnI CGCG 2 cut(s) 936, 1585
MwoI GCNNNNNNNGC 3 cut(s) 502, 1134, 1533
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 1781
NdeI CATATG 1 cut(s) 595
NheI GCTAGC 1 cut(s) 851
NlaIII CATG 5 cut(s) 670, 1223, 1363, 1672, 1785
NlaIV GGNNCC 2 cut(s) 184, 1206
NmeAIII GCCGAG 1 cut(s) 1494
NspI RCATGY 1 cut(s) 670
PagI TCATGA 1 cut(s) 1668
PctI GAATGC 1 cut(s) 1420
PdmI GAANNNNTTC 2 cut(s) 10, 1305
PfeI GAWTC 9 cut(s) 50, 70, 331, 437, 635, 1164, 1444, 1456, 1501
PfoI TCCNGGA 1 cut(s) 1654
PkrI GCNGC 3 cut(s) 320, 948, 951
PleI GAGTC 1 cut(s) 1767
PpsI GAGTC 1 cut(s) 1767
PshBI ATTAAT 1 cut(s) 288
Psp6I CCWGG 1 cut(s) 1654
PspFI CCCAGC 1 cut(s) 1353
PspGI CCWGG 1 cut(s) 1654
PspN4I GGNNCC 2 cut(s) 184, 1206
PspPI GGNCC 3 cut(s) 182, 1466, 1487
PstNI CAGNNNCTG 1 cut(s) 646
PsuI RGATCY 4 cut(s) 191, 228, 840, 938
PvuII CAGCTG 1 cut(s) 1286
RsaI GTAC 1 cut(s) 1801
RsaNI GTAC 1 cut(s) 1800
RseI CAYNNNNRTG 1 cut(s) 573
SaqAI TTAA 7 cut(s) 174, 288, 303, 857, 1562, 1572, 1810
SatI GCNGC 3 cut(s) 319, 947, 950
Sau96I GGNCC 3 cut(s) 182, 1466, 1487
SchI GAGTC 1 cut(s) 1767
ScrFI CCNGG 2 cut(s) 632, 1656
SfaNI GCATC 6 cut(s) 10, 263, 1105, 1390, 1514, 1632
SfcI CTRYAG 1 cut(s) 1014
SinI GGWCC 2 cut(s) 182, 1487
SmiMI CAYNNNNRTG 1 cut(s) 573
SmlI CTYRAG 6 cut(s) 38, 765, 856, 1159, 1370, 1704
SmoI CTYRAG 6 cut(s) 38, 765, 856, 1159, 1370, 1704
SpeI ACTAGT 2 cut(s) 209, 866
Sse9I AATT 5 cut(s) 465, 700, 779, 1377, 1737
SsiI CCGC 4 cut(s) 153, 936, 947, 1055
SspMI CTAG 4 cut(s) 210, 852, 867, 899
StyD4I CCNGG 2 cut(s) 630, 1654
StyI CCWWGG 4 cut(s) 186, 233, 1395, 1781
TaiI ACGT 2 cut(s) 367, 512
TaqI TCGA 9 cut(s) 219, 271, 372, 522, 885, 1338, 1390, 1493, 1678
TasI AATT 5 cut(s) 465, 700, 779, 1377, 1737
TatI WGTACW 1 cut(s) 1799
TauI GCSGC 1 cut(s) 949
TfiI GAWTC 9 cut(s) 50, 70, 331, 437, 635, 1164, 1444, 1456, 1501
Tru1I TTAA 7 cut(s) 174, 288, 303, 857, 1562, 1572, 1810
Tru9I TTAA 7 cut(s) 174, 288, 303, 857, 1562, 1572, 1810
TscAI CASTG 5 cut(s) 301, 456, 828, 1048, 1317
TseI GCWGC 2 cut(s) 318, 949
TspDTI ATGAA 9 cut(s) 42, 582, 693, 744, 803, 1020, 1376, 1685, 1745
TspGWI ACGGA 3 cut(s) 257, 414, 1476
TspRI CASTG 5 cut(s) 301, 456, 828, 1048, 1317
Vha464I CTTAAG 1 cut(s) 856
VpaK11BI GGWCC 2 cut(s) 182, 1487
VspI ATTAAT 1 cut(s) 288
XapI RAATTY 2 cut(s) 700, 1377
XbaI TCTAGA 1 cut(s) 898
XceI RCATGY 1 cut(s) 670
XcmI CCANNNNNNNNNTGG 1 cut(s) 1246
XmiI GTMKAC 1 cut(s) 252
XmnI GAANNNNTTC 2 cut(s) 10, 1305
XspI CTAG 4 cut(s) 210, 852, 867, 899
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.