MD13G1220400.v1.1

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0004960
Physical Location & Seq
Reverse (-)
21320859 .. 21324883
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1220400.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGATGATAAATTTGTAGATGCTCAGAAATACCACGCGGGACAAGAGGATTCATTGCATAGAGTGAGCGAGGAGCGGGAATTATCAGGAGAAGGTACTTTAAATTCGTCTGGCTCAAAATTGAAAAGGTTGGTCACTTGGTCAAATCCACAGCATGCACTAGTTTCAAATGTCAAGCGTTCGCAAATTAGTAAATGTTGCATAAAAGGTTCCTTGTTTCAAAGTGGTATCAGCTTTGACTGTAGGATTCCAAAGCATATACTGAGCTTAGATGAAAAATTTCGTCGTAAATGCATGGAATTGATTCATATTAGTGCATCCAAAGTAGCTCGATGTAATTCAGCTGTGAAATTGAGCTCTTCAAAGATCAGTGCTTTACACGAGCGCTTGAATGTTGCTGAAATTAGAAGTGGAGATACATGTAGTTCTGCTAGATTCGTGATTGAATGTCCATTGGAAGCTGGAGGTGGGAGTGTAGTAATAAGCCCTTCCGGACAGTGGATTGTAGGTAGAGTTATGGGGAGCAAGAGTATGACAAACATATTAAAGAGCCCTTTGTTTCACCAATTTGGGCCCTTAGGTGGCAATACAGACTTGAGAAGAATAGACTGCAATAATGACAAAGGCTCGATATGCTATGATTACACAGACTCACCTGGCAGATTTAGTTTCTCTTCGTCACCGATGCTAGAAAAGGAAAAACCAATTAAGGGAAGCCATAAAAATGAATTTGATGCCGGCCACAAAAGACTTGCTTCTATCTGCAGCACAAACTCAGCGTCATCTGATCAATCTTCTGCTTCTGCTCTCGCCACTGTCTCTCGGGGAATGCTTCAATGCACTTGGAAGGGAGGGAATCCTCATTTTGTTTTCTCCACGGATGAACAAAGGGAGGTCTATGTAGCCAACTTGTGGAAAGTCGAGTCTGAAGAGGATAAGACTCTGGACTATATATATTTGTTTCATTCAGGAAAGGGTGGGCAAAAGGACCATGAGATTCGTGATAGTGAGTCGCAACTTGTTGGTAAGATGAAAGTCAATAATTTGGTCTCCCTCTGCCCAAACAATTCAAAGATCATGGAGACTGAATTTGTTTTGTTTGGTGGTGGTATTGAGATACAAGCTTCTAGCCATAACCTCAGGAAGAGCAAGGGGTTATCAAAAAAGGTGGTTGAGGCGTTCAGGACAACTCACTCATCCAAACAAAGAACCATCCCCAAATTGAGTGGACCGTGTTCCATAATTGAAAATCCTTCTCAGGAGCCATGCCTTGATACAGGCAACAATCAAGATGCTCTAGGTGTGCCTAGCTTGTTAGAAGATCATCTCCCTCCAAATTTCGAATTGGCTTCCATTGTTGTGAAAGACCATCTCCCTGATGATCGAAAGGAGGAAGCTGGAGGTTGGGGTTTGAAATTTCTGAAGAAAGTTGGGAATAAGAAAATCAGTAACTCTGTGGAGGCTTCAGTACCTATCAAATGTTGTTGTAATAATAGTGATTTCTCTACCAGTATGGATGTCCTAGTCCCAGCAGGAATTCACAGTGGGCCAAGGACCAGAAATGGTGGTCCTTCAAGTCTTACTGAAAGATGGAGATCTGGTGGACAGTGTGACTGTGGAGGTTGGGATCTGGGGTGCCCGCTCACAGTACTCAAAACCAAATCAAGCAAAGAAGACATTTTGCCTCAGTCTGACATTCAGGAGGAGTGCAAGTCATTCGAGTTAAATAGAATGGGTTCAGAGCATGGTCCTCCTGCATGGAGGATGTTGAATGTCCATGATGGTTTGTATTTTGTTCATTTTCAACCATCTCTGTCAACTCTGCAGTCTTTCGCAATTGCAGTAGCGATTATCCATGCACAGAGTCCAGCCCTCCGACCCAAATGTACAGGCGTTTTAAGTACCAAAGTCCAGGGTTTGAAAATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

644

Amino Acids

70.4

Weight (kDa)

8.64

Isoelectric Point (pI)

49.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 272 - 621 2.7e-128 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1635
AccB7I CCANNNNNTGG 1 cut(s) 912
AccBSI CCGCTC 2 cut(s) 76, 1642
AccII CGCG 1 cut(s) 38
AccIII TCCGGA 1 cut(s) 491
AciI CCGC 3 cut(s) 38, 76, 1640
AclWI GGATC 1 cut(s) 1635
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 8 cut(s) 11, 103, 278, 728, 1088, 1336, 1415, 1536
AcuI CTGAAG 3 cut(s) 948, 1442, 1449
AfaI GTAC 5 cut(s) 97, 1470, 1650, 1888, 1903
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
AflIII ACRYGT 1 cut(s) 419
AhlI ACTAGT 1 cut(s) 160
AjnI CCWGG 2 cut(s) 655, 1911
AluBI AGCT 9 cut(s) 234, 267, 329, 344, 357, 461, 1124, 1311, 1397
AluI AGCT 9 cut(s) 234, 267, 329, 344, 357, 461, 1124, 1311, 1397
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 3 cut(s) 823, 1054, 1076
AlwI GGATC 1 cut(s) 1635
Ama87I CYCGRG 1 cut(s) 822
Aor13HI TCCGGA 1 cut(s) 491
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 3 cut(s) 572, 739, 1547
ApaI GGGCCC 1 cut(s) 576
ApeKI GCWGC 1 cut(s) 765
ApoI RAATTY 8 cut(s) 11, 103, 278, 728, 1088, 1336, 1415, 1536
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
Asp700I GAANNNNTTC 3 cut(s) 279, 303, 1735
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 8 cut(s) 572, 573, 988, 1229, 1547, 1554, 1568, 1748
AsuHPI GGTGA 3 cut(s) 554, 645, 672
AsuII TTCGAA 1 cut(s) 1341
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 5 cut(s) 988, 1229, 1554, 1568, 1748
AxyI CCTNAGG 2 cut(s) 577, 1139
BaeGI GKGCMC 2 cut(s) 576, 1640
BanI GGYRCC 1 cut(s) 1635
BanII GRGCYC 3 cut(s) 359, 554, 576
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BauI CACGAG 1 cut(s) 380
BbsI GAAGAC 1 cut(s) 1680
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 777
BccI CCATC 5 cut(s) 1220, 1377, 1584, 1775, 1816
BcgI CGANNNNNNTGC 2 cut(s) 1699, 1733
BciT130I CCWGG 2 cut(s) 657, 1913
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 3 cut(s) 823, 1054, 1076
BcuI ACTAGT 1 cut(s) 160
BfaI CTAG 7 cut(s) 161, 432, 689, 1128, 1298, 1308, 1523
BfmI CTRYAG 3 cut(s) 241, 763, 1823
BfoI RGCGCY 1 cut(s) 388
BglII AGATCT 1 cut(s) 1595
BisI GCNGC 1 cut(s) 766
BlsI GCNGC 1 cut(s) 767
BmcAI AGTACT 1 cut(s) 1650
Bme1390I CCNGG 2 cut(s) 657, 1913
Bme18I GGWCC 5 cut(s) 988, 1229, 1554, 1568, 1748
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 8 cut(s) 572, 573, 988, 1229, 1547, 1554, 1568, 1748
BmiI GGNNCC 4 cut(s) 211, 574, 1263, 1637
BmrFI CCNGG 2 cut(s) 657, 1913
BmsI GCATC 5 cut(s) 10, 326, 675, 724, 1282
BpiI GAAGAC 1 cut(s) 1680
BpmI CTGGAG 2 cut(s) 483, 1419
Bpu14I TTCGAA 1 cut(s) 1341
BpuEI CTTGAG 1 cut(s) 616
BsaBI GATNNNNATC 1 cut(s) 1594
BsaI GGTCTC 1 cut(s) 1054
BsaJI CCNNGG 3 cut(s) 876, 1550, 1912
BsaWI WCCGGW 1 cut(s) 491
BsaXI ACNNNNNCTCC 6 cut(s) 514, 544, 1585, 1615, 1697, 1727
Bsc4I CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 1 cut(s) 1509
Bse21I CCTNAGG 2 cut(s) 577, 1139
Bse3DI GCAATG 1 cut(s) 53
Bse8I GATNNNNATC 1 cut(s) 1594
BseAI TCCGGA 1 cut(s) 491
BseBI CCWGG 2 cut(s) 657, 1913
BseDI CCNNGG 3 cut(s) 876, 1550, 1912
BseGI GGATG 7 cut(s) 10, 317, 886, 1196, 1212, 1522, 1770
BseJI GATNNNNATC 1 cut(s) 1594
BseLI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
BseMI GCAATG 1 cut(s) 53
BseMII CTCAG 6 cut(s) 38, 254, 789, 1153, 1271, 1700
BseNI ACTGG 1 cut(s) 1509
BseRI GAGGAG 2 cut(s) 86, 1718
BseSI GKGCMC 2 cut(s) 576, 1640
BseXI GCAGC 1 cut(s) 777
BseYI CCCAGC 1 cut(s) 1528
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 3 cut(s) 574, 741, 1549
BshNI GGYRCC 1 cut(s) 1635
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 2 cut(s) 492, 738
BslFI GGGAC 2 cut(s) 54, 1511
BslI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
BsmAI GTCTC 3 cut(s) 823, 1054, 1076
BsmFI GGGAC 2 cut(s) 54, 1511
BsmI GAATGC 1 cut(s) 834
BsnI GGCC 3 cut(s) 574, 741, 1549
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 1341
Bsp120I GGGCCC 1 cut(s) 572
Bsp1286I GDGCHC 4 cut(s) 359, 554, 576, 1640
Bsp13I TCCGGA 1 cut(s) 491
Bsp1407I TGTACA 1 cut(s) 1886
Bsp143I GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BspACI CCGC 3 cut(s) 38, 76, 1640
BspANI GGCC 3 cut(s) 574, 741, 1549
BspCNI CTCAG 6 cut(s) 37, 255, 788, 1152, 1270, 1699
BspEI TCCGGA 1 cut(s) 491
BspFNI CGCG 1 cut(s) 38
BspLI GGNNCC 4 cut(s) 211, 574, 1263, 1637
BspMAI CTGCAG 2 cut(s) 767, 1827
BspPI GGATC 1 cut(s) 1635
BspQI GCTCTTC 2 cut(s) 364, 1139
BspT104I TTCGAA 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 1635
BspTNI GGTCTC 1 cut(s) 1054
BsrBI CCGCTC 2 cut(s) 76, 1642
BsrDI GCAATG 1 cut(s) 53
BsrFI RCCGGY 1 cut(s) 737
BsrGI TGTACA 1 cut(s) 1886
BsrI ACTGG 1 cut(s) 1509
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 3 cut(s) 876, 1550, 1912
BssMI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BssSI CACGAG 1 cut(s) 380
BssT1I CCWWGG 1 cut(s) 1550
Bst2BI CACGAG 1 cut(s) 380
Bst2UI CCWGG 2 cut(s) 657, 1913
Bst4CI ACNGT 8 cut(s) 242, 498, 817, 1233, 1544, 1608, 1616, 1648
Bst6I CTCTTC 4 cut(s) 364, 679, 924, 1139
BstAUI TGTACA 1 cut(s) 1886
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 3 cut(s) 156, 739, 1640
BstDEI CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1139, 1257, 1686
BstDSI CCRYGG 1 cut(s) 876
BstF5I GGATG 7 cut(s) 10, 317, 886, 1196, 1212, 1522, 1770
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 7 cut(s) 369, 790, 1077, 1324, 1384, 1598, 1630
BstMAI GTCTC 3 cut(s) 823, 1054, 1076
BstMBI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BstMWI GCNNNNNNNGC 1 cut(s) 633
BstNI CCWGG 2 cut(s) 657, 1913
BstNSI RCATGY 2 cut(s) 158, 423
BstSCI CCNGG 2 cut(s) 655, 1911
BstSFI CTRYAG 3 cut(s) 241, 763, 1823
BstSLI GKGCMC 2 cut(s) 576, 1640
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 777
BstV2I GAAGAC 1 cut(s) 1680
BstX2I RGATCY 2 cut(s) 1595, 1627
BstYI RGATCY 2 cut(s) 1595, 1627
Bsu36I CCTNAGG 2 cut(s) 577, 1139
BsuRI GGCC 3 cut(s) 574, 741, 1549
BtgI CCRYGG 1 cut(s) 876
BtsCI GGATG 7 cut(s) 10, 317, 886, 1196, 1212, 1522, 1770
BtsIMutI CAGTG 5 cut(s) 376, 503, 813, 1549, 1613
Cac8I GCNNGC 3 cut(s) 156, 739, 1640
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 8 cut(s) 572, 573, 988, 1229, 1547, 1554, 1568, 1748
CseI GACGC 1 cut(s) 768
Csp6I GTAC 5 cut(s) 96, 1469, 1649, 1887, 1902
CspCI CAANNNNNGTGG 4 cut(s) 1149, 1184, 1207, 1242
CviQI GTAC 5 cut(s) 96, 1469, 1649, 1887, 1902
DdeI CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1139, 1257, 1686
DpnI GATC 7 cut(s) 368, 789, 1076, 1323, 1383, 1597, 1629
DpnII GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
DraI TTTAAA 1 cut(s) 102
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 364, 679, 924, 1139
EarI CTCTTC 4 cut(s) 364, 679, 924, 1139
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 1550
Eco24I GRGCYC 3 cut(s) 359, 554, 576
Eco31I GGTCTC 1 cut(s) 1054
Eco47I GGWCC 5 cut(s) 988, 1229, 1554, 1568, 1748
Eco47III AGCGCT 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 3 cut(s) 948, 1442, 1449
Eco81I CCTNAGG 2 cut(s) 577, 1139
Eco88I CYCGRG 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 573
EcoRI GAATTC 1 cut(s) 1536
EcoRII CCWGG 2 cut(s) 655, 1911
EcoT14I CCWWGG 1 cut(s) 1550
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 3 cut(s) 359, 554, 576
ErhI CCWWGG 1 cut(s) 1550
FalI AAGNNNNNCTT 2 cut(s) 739, 771
FaqI GGGAC 2 cut(s) 54, 1511
FauI CCCGC 3 cut(s) 31, 69, 1647
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 1 cut(s) 766
FokI GGATG 7 cut(s) 17, 304, 893, 1183, 1199, 1529, 1777
FriOI GRGCYC 3 cut(s) 359, 554, 576
Fsp4HI GCNGC 1 cut(s) 766
FspBI CTAG 7 cut(s) 161, 432, 689, 1128, 1298, 1308, 1523
GlaI GCGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 766
GsaI CCCAGC 1 cut(s) 1532
GsuI CTGGAG 2 cut(s) 483, 1419
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 3 cut(s) 574, 741, 1549
HapII CCGG 2 cut(s) 492, 738
HgaI GACGC 1 cut(s) 768
HhaI GCGC 1 cut(s) 387
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HincII GTYRAC 1 cut(s) 1818
HindII GTYRAC 1 cut(s) 1818
HindIII AAGCTT 1 cut(s) 1122
HpaII CCGG 2 cut(s) 492, 738
HphI GGTGA 3 cut(s) 554, 645, 672
Hpy166II GTNNAC 3 cut(s) 1229, 1604, 1818
Hpy188I TCNGA 7 cut(s) 27, 787, 928, 1422, 1693, 1741, 1877
Hpy8I GTNNAC 3 cut(s) 1229, 1604, 1818
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 5 cut(s) 86, 498, 841, 1263, 1581
HpyCH4III ACNGT 8 cut(s) 242, 498, 817, 1233, 1544, 1608, 1616, 1648
HpyF10VI GCNNNNNNNGC 1 cut(s) 633
HpyF3I CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1139, 1257, 1686
HspAI GCGC 1 cut(s) 385
Kpn2I TCCGGA 1 cut(s) 491
KroI GCCGGC 1 cut(s) 737
KroNI GCCGGC 1 cut(s) 739
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
LguI GCTCTTC 2 cut(s) 364, 1139
LmnI GCTCC 3 cut(s) 73, 522, 1261
Lsp1109I GCAGC 1 cut(s) 777
LweI GCATC 5 cut(s) 10, 326, 675, 724, 1282
MaeI CTAG 7 cut(s) 161, 432, 689, 1128, 1298, 1308, 1523
MaeIII GTNAC 4 cut(s) 133, 678, 1448, 1610
MalI GATC 7 cut(s) 368, 789, 1076, 1323, 1383, 1597, 1629
MbiI CCGCTC 2 cut(s) 76, 1642
MboI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
MboII GAAGA 9 cut(s) 351, 612, 666, 786, 941, 1156, 1331, 1435, 1685
MfeI CAATTG 1 cut(s) 1836
MflI RGATCY 2 cut(s) 1595, 1627
MhlI GDGCHC 4 cut(s) 359, 554, 576, 1640
MlyI GAGTC 5 cut(s) 644, 932, 934, 1019, 1873
MmeI TCCRAC 1 cut(s) 1900
Mph1103I ATGCAT 1 cut(s) 296
MroI TCCGGA 1 cut(s) 491
MroNI GCCGGC 1 cut(s) 737
MroXI GAANNNNTTC 3 cut(s) 279, 303, 1735
MseI TTAA 5 cut(s) 101, 545, 708, 1724, 1898
MslI CAYNNNNRTG 3 cut(s) 312, 723, 1358
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 2 cut(s) 492, 738
MspR9I CCNGG 2 cut(s) 657, 1913
MunI CAATTG 1 cut(s) 1836
Mva1269I GAATGC 1 cut(s) 834
MvaI CCWGG 2 cut(s) 657, 1913
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 633
NaeI GCCGGC 1 cut(s) 739
NdeII GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
NgoMIV GCCGGC 1 cut(s) 737
NlaIV GGNNCC 4 cut(s) 211, 574, 1263, 1637
NmuCI GTSAC 3 cut(s) 133, 678, 1610
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 2 cut(s) 158, 423
NspV TTCGAA 1 cut(s) 1341
PaeI GCATGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 419
PciSI GCTCTTC 2 cut(s) 364, 1139
PctI GAATGC 1 cut(s) 834
PdiI GCCGGC 1 cut(s) 739
PdmI GAANNNNTTC 3 cut(s) 279, 303, 1735
PfeI GAWTC 6 cut(s) 50, 247, 304, 435, 856, 997
PflMI CCANNNNNTGG 1 cut(s) 912
PkrI GCNGC 1 cut(s) 767
PleI GAGTC 5 cut(s) 644, 931, 934, 1018, 1872
PpsI GAGTC 5 cut(s) 644, 931, 934, 1018, 1872
PscI ACATGT 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 2 cut(s) 655, 1911
PspFI CCCAGC 1 cut(s) 1528
PspGI CCWGG 2 cut(s) 655, 1911
PspN4I GGNNCC 4 cut(s) 211, 574, 1263, 1637
PspOMI GGGCCC 1 cut(s) 572
PspPI GGNCC 8 cut(s) 572, 573, 988, 1229, 1547, 1554, 1568, 1748
PstI CTGCAG 2 cut(s) 767, 1827
PsuI RGATCY 2 cut(s) 1595, 1627
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 5 cut(s) 97, 1470, 1650, 1888, 1903
RsaNI GTAC 5 cut(s) 96, 1469, 1649, 1887, 1902
RseI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 2 cut(s) 364, 1139
SaqAI TTAA 5 cut(s) 101, 545, 708, 1724, 1898
SatI GCNGC 1 cut(s) 766
Sau3AI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
Sau96I GGNCC 8 cut(s) 572, 573, 988, 1229, 1547, 1554, 1568, 1748
ScaI AGTACT 1 cut(s) 1650
SchI GAGTC 5 cut(s) 644, 932, 934, 1019, 1873
ScrFI CCNGG 2 cut(s) 657, 1913
SduI GDGCHC 4 cut(s) 359, 554, 576, 1640
SfaNI GCATC 5 cut(s) 10, 326, 675, 724, 1282
SfcI CTRYAG 3 cut(s) 241, 763, 1823
SfuI TTCGAA 1 cut(s) 1341
SinI GGWCC 5 cut(s) 988, 1229, 1554, 1568, 1748
SmiMI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SpeI ACTAGT 1 cut(s) 160
SphI GCATGC 1 cut(s) 158
SsiI CCGC 3 cut(s) 38, 76, 1640
SspMI CTAG 7 cut(s) 161, 432, 689, 1128, 1298, 1308, 1523
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 2 cut(s) 655, 1911
StyI CCWWGG 1 cut(s) 1550
TaaI ACNGT 8 cut(s) 242, 498, 817, 1233, 1544, 1608, 1616, 1648
TaqI TCGA 6 cut(s) 331, 629, 921, 1341, 1384, 1719
TatI WGTACW 2 cut(s) 1648, 1886
TfiI GAWTC 6 cut(s) 50, 247, 304, 435, 856, 997
Tru1I TTAA 5 cut(s) 101, 545, 708, 1724, 1898
Tru9I TTAA 5 cut(s) 101, 545, 708, 1724, 1898
TscAI CASTG 5 cut(s) 376, 503, 820, 1549, 1613
TseFI GTSAC 3 cut(s) 133, 678, 1610
TseI GCWGC 1 cut(s) 765
Tsp45I GTSAC 3 cut(s) 133, 678, 1610
TspDTI ATGAA 8 cut(s) 42, 288, 296, 741, 897, 953, 1046, 1787
TspGWI ACGGA 1 cut(s) 893
TspRI CASTG 5 cut(s) 376, 503, 820, 1549, 1613
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 5 cut(s) 988, 1229, 1554, 1568, 1748
XapI RAATTY 8 cut(s) 11, 103, 278, 728, 1088, 1336, 1415, 1536
XceI RCATGY 2 cut(s) 158, 423
XmnI GAANNNNTTC 3 cut(s) 279, 303, 1735
XspI CTAG 7 cut(s) 161, 432, 689, 1128, 1298, 1308, 1523
ZrmI AGTACT 1 cut(s) 1650
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.