Rw4G004650

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0004960
Physical Location & Seq
Reverse (-)
9183445 .. 9187717
4273 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G004650.1

Sequence Viewer

Length: 1908 bp
ATGGATGATCAAGTTATAATTCCTCAAAAAGATCGCCGGGGAAAAGAGGATACATCTCATAGATTGAGCAAGGAGAGAGTATTATCAAGAGAAGGTACTTTAAAATCCTCTGGCTCAAAATTGAAGAGGTTGGTCAAAATGGCAAATCCACAGCACCCTATAATTTCAAATGTTAATAGTTCACAAATTAGTAGATGTTGCGCAAAAAGTCCATTGTTTCAAAGTACTGTTGGTGTTGAGAGTAGGATTCCAAAGCATATACTGAGCGTAGATGAAAAATTTCGCCGTCGATGCTTGGAATTGATTCATATTAGTGCATCCAAAGCAGCTCAATGCAACGTAGCTGTGAATTTGAGCTCTGCGAAGATGGGTGTTTTAGCTGAAAGCCTTAGTGCGGGTGATATTAGAAGTGGAGATACATGTGACTCTGCGAGGTTTGTTTATGAATGCCCATTGGAAGCTGGAGGTGGGAGTGTTGTCATAAGCCCTGCAGGGCAGTGGATTGTAGGTACAGTTATGGGGAGCAAGAGCATGATAAATATTTTGAAGAGCCCTTTGTTTCACCAAGTTGGCCCCGAAGGTGGCAATGCAGATTCTAGAAGAATAACTTCAAATGATGACAAAGCCTCGATGTGCAATGATTTAGCAGAGTGTCCTGGTGCATTAACTTTCTCTTCACCGAAGACAGAAAAAGAAATTCCAGTTAAGGGAAGCATTAAAAATGAATCTGATGGGGGTCACAAAAGGCTTGTTTCTACGTGTAGCATAGACTCTCCATCTTCTGATCAATCTTCCCGTGCGGCTCTCGCCACCGTTTCCCAAGGAATGCTCCAGTGCACGTGGAAGGGAGGGAATCCTCATTTTGTTTTCTCTACAGATGAACAGAAGGAGGTCTATGTAGCCAATTTGTGGAAGGTTCAGTCTAAAGAGGACAAGTCTCTGGATTATATATACTTGTTCCATGCAGGAAAGGGTGGCCAAAAGAACCATGAGATTCGCGATAGTGAGTCACACCTAGTCGGTAAGATGAAGGTGAGTAATTTGGTCTCTCTCTGCTCAAACAATTCAAAGATCATGGAGACTGAGTTTGTTTTGTATGGTGATGGTATTGAGATGCACACTTCTAGCCATAATCTCAAGAAGAGTAAGGGGTTATCGAAAAAGATGGTGGGAGTGTTCAGGACAAGTCACTTATCCAAACAAAAAACCATCTCCAAATTGAGTGGAGCTCTTGAGAATTCTTCTTGGGAGTCATGCCTAGATGATACAGCCAGCAATCAAGATGCTCTAGGTCTGCCGAATTTGTTAGAAGATAACCTCCCTCCAAATTTTGAATTAGCTGCAATTGTTATGAAAGACCATCTCCCTGATGATCGTAAGGAGGAAACTGGAGGTTGGGGTTTGAAATTTCTGAAGAAAGTTGGGGTGAAGAAAACCAATAACTCTGTGGAAGTGTCAGTGCCTCTCGAATGTTGTCGAAATAATGGTGATTGCTCTACAAGTATGGATGTTCTAATCCCCGCAGGTCTTCATGGTGGACCAAGAACCAGAAATGGTGGCCCTTCAAGTCTTACTGAAAGATGGAGATCTGGTGGATACTGTGACTGTGGTGGCTGGGACATGGGGTGCCCTCTCACAGTACTTAAAAGCAAATCAAGCAAAGAGGACATTTTGCCTCAGTCAGATACTCAAGGGGAGTGCAAGCCGTTTGATTTAAATTTACAGGGCTCTGAGCATGCCACTCCCACATTAAGGATGCTGAATGTCCATGATGGTCTGTATTTTGTTCATTTTCAACCTACTCTATCAATTCTGCAATCTTTCTCAATTGCAGTAGCGATTATCCATACACAGACTCCTTCTCTCCGGCCCAAATGTACAGAAGTTTCAACCAAAGTCCAGGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

635

Amino Acids

69.04

Weight (kDa)

8.54

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 271 - 618 9.6e-133 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
Acc16I TGCGCA 1 cut(s) 202
Acc36I ACCTGC 1 cut(s) 1514
AccB1I GGYRCC 1 cut(s) 1626
AccB7I CCANNNNNTGG 1 cut(s) 909
AccII CGCG 1 cut(s) 999
AciI CCGC 3 cut(s) 395, 800, 1521
AcoI YGGCCR 1 cut(s) 976
AcsI RAATTY 8 cut(s) 278, 349, 696, 1237, 1300, 1327, 1406, 1717
AcuI CTGAAG 1 cut(s) 1433
AcvI CACGTG 1 cut(s) 840
AfaI GTAC 5 cut(s) 97, 226, 511, 1641, 1879
AfiI CCNNNNNNNGG 5 cut(s) 394, 581, 707, 909, 1752
AflIII ACRYGT 2 cut(s) 419, 758
AjnI CCWGG 2 cut(s) 655, 1899
AluBI AGCT 7 cut(s) 329, 344, 357, 380, 461, 1229, 1340
AluI AGCT 7 cut(s) 329, 344, 357, 380, 461, 1229, 1340
Alw21I GWGCWC 3 cut(s) 359, 839, 1231
Alw26I GTCTC 3 cut(s) 942, 1051, 1073
Alw44I GTGCAC 1 cut(s) 835
AoxI GGCC 4 cut(s) 571, 976, 1558, 1868
ApaLI GTGCAC 1 cut(s) 835
ApeKI GCWGC 2 cut(s) 326, 1340
ApoI RAATTY 8 cut(s) 278, 349, 696, 1237, 1300, 1327, 1406, 1717
Asp700I GAANNNNTTC 3 cut(s) 279, 303, 607
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 4 cut(s) 572, 1538, 1559, 1869
AsuC2I CCSGG 1 cut(s) 38
AsuHPI GGTGA 7 cut(s) 410, 554, 669, 1045, 1112, 1438, 1499
AvaII GGWCC 1 cut(s) 1538
BaeGI GKGCMC 2 cut(s) 839, 1631
BalI TGGCCA 1 cut(s) 978
BanI GGYRCC 1 cut(s) 1626
BanII GRGCYC 4 cut(s) 359, 554, 1231, 1730
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbrPI CACGTG 1 cut(s) 840
BbsI GAAGAC 2 cut(s) 689, 1520
Bbv12I GWGCWC 3 cut(s) 359, 839, 1231
BbvI GCAGC 2 cut(s) 338, 1327
BccI CCATC 9 cut(s) 361, 725, 784, 1097, 1159, 1217, 1368, 1575, 1766
BceAI ACGGC 2 cut(s) 270, 1690
BciT130I CCWGG 2 cut(s) 657, 1901
BciVI GTATCC 2 cut(s) 43, 1589
BclI TGATCA 2 cut(s) 7, 784
BcnI CCSGG 1 cut(s) 38
BcoDI GTCTC 3 cut(s) 942, 1051, 1073
BfaI CTAG 5 cut(s) 597, 1016, 1125, 1259, 1289
BfmI CTRYAG 2 cut(s) 489, 873
BfuAI ACCTGC 1 cut(s) 1514
BfuI GTATCC 2 cut(s) 43, 1589
BglII AGATCT 1 cut(s) 1586
BisI GCNGC 3 cut(s) 327, 801, 1341
BlsI GCNGC 3 cut(s) 328, 802, 1342
BmcAI AGTACT 2 cut(s) 226, 1641
Bme1390I CCNGG 3 cut(s) 38, 657, 1901
Bme18I GGWCC 1 cut(s) 1538
BmgT120I GGNCC 4 cut(s) 572, 1538, 1559, 1869
BmiI GGNNCC 2 cut(s) 574, 1628
BmrFI CCNGG 3 cut(s) 38, 657, 1901
BmsI GCATC 5 cut(s) 281, 326, 1104, 1273, 1746
BpiI GAAGAC 2 cut(s) 689, 1520
BplI GAGNNNNNCTC 2 cut(s) 1213, 1245
BpmI CTGGAG 3 cut(s) 483, 815, 1410
BpuEI CTTGAG 3 cut(s) 1121, 1253, 1674
BpuMI CCSGG 1 cut(s) 38
BsaAI YACGTR 2 cut(s) 759, 840
BsaBI GATNNNNATC 1 cut(s) 1585
BsaI GGTCTC 1 cut(s) 1051
BsaJI CCNNGG 3 cut(s) 37, 820, 1900
BsaXI ACNNNNNCTCC 6 cut(s) 1070, 1100, 1576, 1606, 1840, 1870
Bsc4I CCNNNNNNNGG 5 cut(s) 394, 581, 707, 909, 1752
Bse1I ACTGG 3 cut(s) 701, 832, 1393
Bse3DI GCAATG 2 cut(s) 592, 643
Bse8I GATNNNNATC 1 cut(s) 1585
BseBI CCWGG 2 cut(s) 657, 1901
BseDI CCNNGG 3 cut(s) 37, 820, 1900
BseGI GGATG 4 cut(s) 10, 317, 1513, 1761
BseJI GATNNNNATC 1 cut(s) 1585
BseLI CCNNNNNNNGG 5 cut(s) 394, 581, 707, 909, 1752
BseMI GCAATG 2 cut(s) 592, 643
BseMII CTCAG 4 cut(s) 254, 1074, 1691, 1722
BseNI ACTGG 3 cut(s) 701, 832, 1393
BseSI GKGCMC 2 cut(s) 839, 1631
BseXI GCAGC 2 cut(s) 338, 1327
BseYI CCCAGC 1 cut(s) 1614
Bsh1236I CGCG 1 cut(s) 999
BshFI GGCC 4 cut(s) 573, 978, 1560, 1870
BshNI GGYRCC 1 cut(s) 1626
BsiHKAI GWGCWC 3 cut(s) 359, 839, 1231
BsiSI CCGG 2 cut(s) 37, 1867
BslFI GGGAC 1 cut(s) 1631
BslI CCNNNNNNNGG 5 cut(s) 394, 581, 707, 909, 1752
BsmAI GTCTC 3 cut(s) 942, 1051, 1073
BsmFI GGGAC 1 cut(s) 1631
BsmI GAATGC 2 cut(s) 452, 831
BsnI GGCC 4 cut(s) 573, 978, 1560, 1870
Bso31I GGTCTC 1 cut(s) 1051
Bsp1286I GDGCHC 6 cut(s) 359, 554, 839, 1231, 1631, 1730
Bsp1407I TGTACA 1 cut(s) 1877
Bsp143I GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
Bsp68I TCGCGA 1 cut(s) 999
BspACI CCGC 3 cut(s) 395, 800, 1521
BspANI GGCC 4 cut(s) 573, 978, 1560, 1870
BspCNI CTCAG 4 cut(s) 255, 1075, 1690, 1723
BspFNI CGCG 1 cut(s) 999
BspLI GGNNCC 2 cut(s) 574, 1628
BspMAI CTGCAG 1 cut(s) 493
BspMI ACCTGC 1 cut(s) 1514
BspQI GCTCTTC 1 cut(s) 542
BspT107I GGYRCC 1 cut(s) 1626
BspTNI GGTCTC 1 cut(s) 1051
BsrDI GCAATG 2 cut(s) 592, 643
BsrGI TGTACA 1 cut(s) 1877
BsrI ACTGG 3 cut(s) 701, 832, 1393
BssECI CCNNGG 3 cut(s) 37, 820, 1900
BssMI GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
BssT1I CCWWGG 1 cut(s) 820
Bst2UI CCWGG 2 cut(s) 657, 1901
Bst4CI ACNGT 6 cut(s) 229, 514, 814, 1601, 1607, 1639
Bst6I CTCTTC 4 cut(s) 119, 542, 679, 1136
BstAUI TGTACA 1 cut(s) 1877
BstBAI YACGTR 2 cut(s) 759, 840
BstC8I GCNNGC 3 cut(s) 1273, 1703, 1737
BstDEI CTNAG 5 cut(s) 263, 389, 1083, 1677, 1731
BstF5I GGATG 4 cut(s) 10, 317, 1513, 1761
BstFNI CGCG 1 cut(s) 999
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 6 cut(s) 10, 34, 787, 1074, 1375, 1589
BstMAI GTCTC 3 cut(s) 942, 1051, 1073
BstMBI GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
BstMWI GCNNNNNNNGC 4 cut(s) 291, 323, 806, 1656
BstNI CCWGG 2 cut(s) 657, 1901
BstNSI RCATGY 2 cut(s) 423, 1739
BstSCI CCNGG 3 cut(s) 36, 655, 1899
BstSFI CTRYAG 2 cut(s) 489, 873
BstSLI GKGCMC 2 cut(s) 839, 1631
BstUI CGCG 1 cut(s) 999
BstV1I GCAGC 2 cut(s) 338, 1327
BstV2I GAAGAC 2 cut(s) 689, 1520
BstX2I RGATCY 1 cut(s) 1586
BstYI RGATCY 1 cut(s) 1586
BsuI GTATCC 2 cut(s) 43, 1589
BsuRI GGCC 4 cut(s) 573, 978, 1560, 1870
BtsCI GGATG 4 cut(s) 10, 317, 1513, 1761
BtsI GCAGTG 1 cut(s) 503
BtsIMutI CAGTG 3 cut(s) 503, 839, 1464
BtuMI TCGCGA 1 cut(s) 999
BveI ACCTGC 1 cut(s) 1514
Cac8I GCNNGC 3 cut(s) 1273, 1703, 1737
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 4 cut(s) 572, 1538, 1559, 1869
Csp6I GTAC 5 cut(s) 96, 225, 510, 1640, 1878
CspCI CAANNNNNGTGG 2 cut(s) 1204, 1239
CviQI GTAC 5 cut(s) 96, 225, 510, 1640, 1878
DdeI CTNAG 5 cut(s) 263, 389, 1083, 1677, 1731
DpnI GATC 6 cut(s) 9, 33, 786, 1073, 1374, 1588
DpnII GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
DraI TTTAAA 2 cut(s) 102, 1716
EaeI YGGCCR 1 cut(s) 976
Eam1104I CTCTTC 4 cut(s) 119, 542, 679, 1136
EarI CTCTTC 4 cut(s) 119, 542, 679, 1136
Ecl136II GAGCTC 2 cut(s) 357, 1229
Eco130I CCWWGG 1 cut(s) 820
Eco24I GRGCYC 4 cut(s) 359, 554, 1231, 1730
Eco31I GGTCTC 1 cut(s) 1051
Eco47I GGWCC 1 cut(s) 1538
Eco53kI GAGCTC 2 cut(s) 357, 1229
Eco57I CTGAAG 1 cut(s) 1433
Eco72I CACGTG 1 cut(s) 840
EcoICRI GAGCTC 2 cut(s) 357, 1229
EcoRI GAATTC 1 cut(s) 1237
EcoRII CCWGG 2 cut(s) 655, 1899
EcoT14I CCWWGG 1 cut(s) 820
EcoT38I GRGCYC 4 cut(s) 359, 554, 1231, 1730
ErhI CCWWGG 1 cut(s) 820
FalI AAGNNNNNCTT 2 cut(s) 592, 624
FaqI GGGAC 1 cut(s) 1631
FauI CCCGC 2 cut(s) 388, 1528
FbaI TGATCA 2 cut(s) 7, 784
Fnu4HI GCNGC 3 cut(s) 327, 801, 1341
FokI GGATG 4 cut(s) 17, 304, 1520, 1768
FriOI GRGCYC 4 cut(s) 359, 554, 1231, 1730
Fsp4HI GCNGC 3 cut(s) 327, 801, 1341
FspBI CTAG 5 cut(s) 597, 1016, 1125, 1259, 1289
FspI TGCGCA 1 cut(s) 202
GlaI GCGC 1 cut(s) 202
GluI GCNGC 3 cut(s) 327, 801, 1341
GsaI CCCAGC 1 cut(s) 1618
GsuI CTGGAG 3 cut(s) 483, 815, 1410
HaeIII GGCC 4 cut(s) 573, 978, 1560, 1870
HapII CCGG 2 cut(s) 37, 1867
HhaI GCGC 1 cut(s) 203
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HpaII CCGG 2 cut(s) 37, 1867
HphI GGTGA 7 cut(s) 410, 554, 669, 1045, 1112, 1438, 1499
Hpy166II GTNNAC 3 cut(s) 182, 837, 1538
Hpy188I TCNGA 5 cut(s) 730, 784, 1413, 1684, 1732
Hpy188III TCNNGA 9 cut(s) 87, 597, 941, 998, 1138, 1180, 1232, 1280, 1466
Hpy8I GTNNAC 3 cut(s) 182, 837, 1538
Hpy99I CGWCG 1 cut(s) 291
HpyAV CCTTC 8 cut(s) 86, 572, 838, 880, 907, 1024, 1572, 1869
HpyCH4III ACNGT 6 cut(s) 229, 514, 814, 1601, 1607, 1639
HpyCH4IV ACGT 3 cut(s) 339, 758, 839
HpyF10VI GCNNNNNNNGC 4 cut(s) 291, 323, 806, 1656
HpyF3I CTNAG 5 cut(s) 263, 389, 1083, 1677, 1731
HpySE526I ACGT 3 cut(s) 339, 758, 839
HspAI GCGC 1 cut(s) 201
Ksp22I TGATCA 2 cut(s) 7, 784
Kzo9I GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
LguI GCTCTTC 1 cut(s) 542
LmnI GCTCC 3 cut(s) 522, 834, 1226
Lsp1109I GCAGC 2 cut(s) 338, 1327
LweI GCATC 5 cut(s) 281, 326, 1104, 1273, 1746
MaeI CTAG 5 cut(s) 597, 1016, 1125, 1259, 1289
MaeII ACGT 3 cut(s) 339, 758, 839
MaeIII GTNAC 5 cut(s) 422, 737, 1008, 1187, 1601
MalI GATC 6 cut(s) 9, 33, 786, 1073, 1374, 1588
MboI GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
MfeI CAATTG 2 cut(s) 1344, 1827
MflI RGATCY 1 cut(s) 1586
MhlI GDGCHC 6 cut(s) 359, 554, 839, 1231, 1631, 1730
MlsI TGGCCA 1 cut(s) 978
MluNI TGGCCA 1 cut(s) 978
MlyI GAGTC 5 cut(s) 419, 764, 1016, 1259, 1849
Mox20I TGGCCA 1 cut(s) 978
MroXI GAANNNNTTC 3 cut(s) 279, 303, 607
MscI TGGCCA 1 cut(s) 978
MseI TTAA 8 cut(s) 101, 174, 665, 705, 717, 1644, 1715, 1751
MslI CAYNNNNRTG 1 cut(s) 312
Msp20I TGGCCA 1 cut(s) 978
MspI CCGG 2 cut(s) 37, 1867
MspR9I CCNGG 3 cut(s) 38, 657, 1901
MunI CAATTG 2 cut(s) 1344, 1827
Mva1269I GAATGC 2 cut(s) 452, 831
MvaI CCWGG 2 cut(s) 657, 1901
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 4 cut(s) 291, 323, 806, 1656
NciI CCSGG 1 cut(s) 38
NdeII GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
NlaIV GGNNCC 2 cut(s) 574, 1628
NmuCI GTSAC 5 cut(s) 422, 737, 1008, 1187, 1601
NruI TCGCGA 1 cut(s) 999
NsbI TGCGCA 1 cut(s) 202
NspI RCATGY 2 cut(s) 423, 1739
PaeI GCATGC 1 cut(s) 1739
PciI ACATGT 1 cut(s) 419
PciSI GCTCTTC 1 cut(s) 542
PctI GAATGC 2 cut(s) 452, 831
PdmI GAANNNNTTC 3 cut(s) 279, 303, 607
PfeI GAWTC 6 cut(s) 247, 304, 593, 725, 853, 994
PflMI CCANNNNNTGG 1 cut(s) 909
PkrI GCNGC 3 cut(s) 328, 802, 1342
PleI GAGTC 5 cut(s) 419, 764, 1015, 1258, 1849
PmaCI CACGTG 1 cut(s) 840
PmlI CACGTG 1 cut(s) 840
PpsI GAGTC 5 cut(s) 419, 764, 1015, 1258, 1849
Ppu21I YACGTR 2 cut(s) 759, 840
PscI ACATGT 1 cut(s) 419
PsiI TTATAA 1 cut(s) 17
Psp124BI GAGCTC 2 cut(s) 359, 1231
Psp6I CCWGG 2 cut(s) 655, 1899
PspCI CACGTG 1 cut(s) 840
PspFI CCCAGC 1 cut(s) 1614
PspGI CCWGG 2 cut(s) 655, 1899
PspN4I GGNNCC 2 cut(s) 574, 1628
PspPI GGNCC 4 cut(s) 572, 1538, 1559, 1869
PstI CTGCAG 1 cut(s) 493
PsuI RGATCY 1 cut(s) 1586
RruI TCGCGA 1 cut(s) 999
RsaI GTAC 5 cut(s) 97, 226, 511, 1641, 1879
RsaNI GTAC 5 cut(s) 96, 225, 510, 1640, 1878
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 2 cut(s) 359, 1231
SapI GCTCTTC 1 cut(s) 542
SaqAI TTAA 8 cut(s) 101, 174, 665, 705, 717, 1644, 1715, 1751
SatI GCNGC 3 cut(s) 327, 801, 1341
Sau3AI GATC 6 cut(s) 7, 31, 784, 1071, 1372, 1586
Sau96I GGNCC 4 cut(s) 572, 1538, 1559, 1869
SbfI CCTGCAGG 1 cut(s) 493
ScaI AGTACT 2 cut(s) 226, 1641
SchI GAGTC 5 cut(s) 419, 764, 1016, 1259, 1849
ScrFI CCNGG 3 cut(s) 38, 657, 1901
SdaI CCTGCAGG 1 cut(s) 493
SduI GDGCHC 6 cut(s) 359, 554, 839, 1231, 1631, 1730
SfaNI GCATC 5 cut(s) 281, 326, 1104, 1273, 1746
SfcI CTRYAG 2 cut(s) 489, 873
SinI GGWCC 1 cut(s) 1538
SmiI ATTTAAAT 1 cut(s) 1716
SmiMI CAYNNNNRTG 1 cut(s) 312
SmlI CTYRAG 3 cut(s) 1136, 1232, 1689
SmoI CTYRAG 3 cut(s) 1136, 1232, 1689
SphI GCATGC 1 cut(s) 1739
Sse8387I CCTGCAGG 1 cut(s) 493
SsiI CCGC 3 cut(s) 395, 800, 1521
SspI AATATT 1 cut(s) 541
SspMI CTAG 5 cut(s) 597, 1016, 1125, 1259, 1289
SstI GAGCTC 2 cut(s) 359, 1231
StyD4I CCNGG 3 cut(s) 36, 655, 1899
StyI CCWWGG 1 cut(s) 820
SwaI ATTTAAAT 1 cut(s) 1716
TaaI ACNGT 6 cut(s) 229, 514, 814, 1601, 1607, 1639
TaiI ACGT 3 cut(s) 342, 761, 842
TaqI TCGA 5 cut(s) 289, 629, 1157, 1467, 1477
TatI WGTACW 3 cut(s) 224, 1639, 1877
TauI GCSGC 1 cut(s) 803
TfiI GAWTC 6 cut(s) 247, 304, 593, 725, 853, 994
Tru1I TTAA 8 cut(s) 101, 174, 665, 705, 717, 1644, 1715, 1751
Tru9I TTAA 8 cut(s) 101, 174, 665, 705, 717, 1644, 1715, 1751
TscAI CASTG 3 cut(s) 503, 839, 1464
TseFI GTSAC 5 cut(s) 422, 737, 1008, 1187, 1601
TseI GCWGC 2 cut(s) 326, 1340
Tsp45I GTSAC 5 cut(s) 422, 737, 1008, 1187, 1601
TspDTI ATGAA 9 cut(s) 288, 296, 459, 738, 894, 1043, 1367, 1520, 1778
TspRI CASTG 3 cut(s) 503, 839, 1464
Van91I CCANNNNNTGG 1 cut(s) 909
VneI GTGCAC 1 cut(s) 835
VpaK11BI GGWCC 1 cut(s) 1538
XapI RAATTY 8 cut(s) 278, 349, 696, 1237, 1300, 1327, 1406, 1717
XbaI TCTAGA 1 cut(s) 596
XceI RCATGY 2 cut(s) 423, 1739
XcmI CCANNNNNNNNNTGG 1 cut(s) 1444
XmnI GAANNNNTTC 3 cut(s) 279, 303, 607
XspI CTAG 5 cut(s) 597, 1016, 1125, 1259, 1289
ZrmI AGTACT 2 cut(s) 226, 1641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.