pycom13g19500

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0004960
Physical Location & Seq
Reverse (-)
15475109 .. 15476488
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19500.1

Sequence Viewer

Length: 1380 bp
ATGGATGATAAATTTGTAGATGCTCAGAAATACCACGCGGGACAAGAGGATTCATTGCATACAGTGAGCGAGGAGCGGGAATCATCAGGAGAAGGTACTTTAAATTCGACTGGCTCAAAATTGAAAAGGTTGATCACATGGTCAAATCCACAGCATGCACTAATTTCAAATGTTAAGCGTTCGCAAATTAGTAAATGTTGCATAAAAGGTTCCTTGTTTCAAAGTGGTATTAGCTTTGACTGTAGGATTCCAAAGCATATACTGAGCTTAGATGAAAAATTTCGTCGTAAATGCATGGAATTGATTCATATTAGTGCATTCAAAGTAGCTCGATGTAATTCAGCTGTGAAATCGAGCTCTTCAAAGATCAGTGCTTTGCACGAGCGCTTGAATGTTGCTGAAATTAGAAGTGGAGATACATGTAGCTCTGCTAGATTCGTGATTGAATGTCCATTGGAAGCTGGAGGTGGGAGTGTAGTAATAAGCCCTTCTGGACAGTGGATTGTAGGTAGAGTTATGGGGAGCAAGAGTATGACAAACATATTAAAGAGCCCTTTGTTTCACCAATTTGGGCCCTTAGGTGGCAATGCAGACTTGAGAAGAATAGACTGCAATAATGACAAAGGCTCGATATGCTATGATTACACAGACTCACCTGGCGGATTTAGTTTCTCTTCATCACCGATGCTAGAAAAGGAAAAACCAATTAAGGGAAGCCATAAAAATGAATTTGATGCCGGCCACAAAAGACTTGCTTCTATCTGCAGCACAAACTCAGCGTCATCTGATCAATCTTCTGCTTCTGCTTTCGCCACTGTCTCTCGGGGAATGCTTCAATGCACTTGGAAGGGAGGGAATCCTCATTTTGTTTTCTCCACAGATGAACAAGGGGAGGTCTATGTAGCCAACTTGTGGAAAGTCGAGTCCGAAGAGGATAAGACTCTGGACTATATATATTTGTTTCATTCAGGAAAGGGTGGGCAAAAGGACCATGAGATTCGTGATACTGAGTCGCAACTTGTTGGTAAGATGAAAGTCAATAATTTGGTCTCCCTCTGCCCAAACAATTCAAAGATCATGGAGACTGAATTTGTTTTGTTTGGTGGTGGTATTGAGATACAAGCTTCTAGCCATAACCTCAGGAAGAGCAAGGGGTTATCGAAAAAGGTGGTTGAGGCGTTCAGGACAACTCACTCATCCAAACAAAGAACCATCTCCAAATTGAGTGGACCGTGCTCCATAGTTGAAAATCCTTCTCAGGAGCCATGCCTTGATACAGGCAACAATCATGATGCTCTAGGTGTGCCCAACTTGTTAGAAGATCGATCTCCCTCCAAATTTCGAATTGGCTTCCATTGTTGTGAAAGACCATCTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

460

Amino Acids

50.34

Weight (kDa)

8.59

Isoelectric Point (pI)

49.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 272 - 446 1.1e-45 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 912
AccBSI CCGCTC 1 cut(s) 76
AccII CGCG 1 cut(s) 38
AciI CCGC 3 cut(s) 38, 76, 660
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 6 cut(s) 11, 103, 278, 728, 1088, 1337
AfaI GTAC 1 cut(s) 97
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 3 cut(s) 581, 710, 912
AflIII ACRYGT 1 cut(s) 419
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 8 cut(s) 234, 267, 329, 344, 357, 426, 461, 1124
AluI AGCT 8 cut(s) 234, 267, 329, 344, 357, 426, 461, 1124
Alw21I GWGCWC 2 cut(s) 359, 1238
Alw26I GTCTC 3 cut(s) 823, 1054, 1076
Ama87I CYCGRG 1 cut(s) 822
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 2 cut(s) 572, 739
ApaI GGGCCC 1 cut(s) 576
ApeKI GCWGC 1 cut(s) 765
ApoI RAATTY 6 cut(s) 11, 103, 278, 728, 1088, 1337
ArsI GACNNNNNNTTYG 2 cut(s) 764, 796
Asp700I GAANNNNTTC 2 cut(s) 279, 303
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 4 cut(s) 572, 573, 988, 1229
AsuHPI GGTGA 3 cut(s) 554, 645, 672
AsuII TTCGAA 1 cut(s) 1342
AvaI CYCGRG 1 cut(s) 822
AvaII GGWCC 2 cut(s) 988, 1229
AxyI CCTNAGG 2 cut(s) 577, 1139
BaeGI GKGCMC 2 cut(s) 576, 1308
BanII GRGCYC 3 cut(s) 359, 554, 576
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BauI CACGAG 1 cut(s) 380
Bbv12I GWGCWC 2 cut(s) 359, 1238
BbvI GCAGC 1 cut(s) 777
BccI CCATC 2 cut(s) 1220, 1378
BciT130I CCWGG 1 cut(s) 657
BclI TGATCA 2 cut(s) 132, 787
BcoDI GTCTC 3 cut(s) 823, 1054, 1076
BfaI CTAG 4 cut(s) 432, 689, 1128, 1298
BfmI CTRYAG 2 cut(s) 241, 763
BfoI RGCGCY 1 cut(s) 388
BisI GCNGC 1 cut(s) 766
BlsI GCNGC 1 cut(s) 767
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 2 cut(s) 988, 1229
BmeT110I CYCGRG 1 cut(s) 822
BmgT120I GGNCC 4 cut(s) 572, 573, 988, 1229
BmiI GGNNCC 3 cut(s) 211, 574, 1263
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 4 cut(s) 10, 675, 724, 1282
BpmI CTGGAG 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 1342
BpuEI CTTGAG 1 cut(s) 616
Bsa29I ATCGAT 1 cut(s) 1324
BsaI GGTCTC 1 cut(s) 1054
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 3 cut(s) 581, 710, 912
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 1 cut(s) 115
Bse21I CCTNAGG 2 cut(s) 577, 1139
Bse3DI GCAATG 2 cut(s) 53, 592
BseBI CCWGG 1 cut(s) 657
BseCI ATCGAT 1 cut(s) 1324
BseGI GGATG 2 cut(s) 10, 1196
BseLI CCNNNNNNNGG 3 cut(s) 581, 710, 912
BseMI GCAATG 2 cut(s) 53, 592
BseMII CTCAG 6 cut(s) 38, 254, 789, 999, 1153, 1271
BseNI ACTGG 1 cut(s) 115
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 2 cut(s) 576, 1308
BseXI GCAGC 1 cut(s) 777
Bsh1236I CGCG 1 cut(s) 38
BshFI GGCC 2 cut(s) 574, 741
BshVI ATCGAT 1 cut(s) 1324
BsiHKAI GWGCWC 2 cut(s) 359, 1238
BsiHKCI CYCGRG 1 cut(s) 822
BsiSI CCGG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 54
BslI CCNNNNNNNGG 3 cut(s) 581, 710, 912
BsmAI GTCTC 3 cut(s) 823, 1054, 1076
BsmFI GGGAC 1 cut(s) 54
BsmI GAATGC 2 cut(s) 317, 834
BsnI GGCC 2 cut(s) 574, 741
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 1 cut(s) 822
Bsp119I TTCGAA 1 cut(s) 1342
Bsp120I GGGCCC 1 cut(s) 572
Bsp1286I GDGCHC 5 cut(s) 359, 554, 576, 1238, 1308
Bsp143I GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
BspACI CCGC 3 cut(s) 38, 76, 660
BspANI GGCC 2 cut(s) 574, 741
BspCNI CTCAG 6 cut(s) 37, 255, 788, 1000, 1152, 1270
BspDI ATCGAT 1 cut(s) 1324
BspFNI CGCG 1 cut(s) 38
BspHI TCATGA 1 cut(s) 1288
BspLI GGNNCC 3 cut(s) 211, 574, 1263
BspMAI CTGCAG 1 cut(s) 767
BspQI GCTCTTC 2 cut(s) 364, 1139
BspT104I TTCGAA 1 cut(s) 1342
BspTNI GGTCTC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 76
BsrDI GCAATG 2 cut(s) 53, 592
BsrFI RCCGGY 1 cut(s) 737
BsrI ACTGG 1 cut(s) 115
BssAI RCCGGY 1 cut(s) 737
BssMI GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
BssSI CACGAG 1 cut(s) 380
Bst2BI CACGAG 1 cut(s) 380
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 5 cut(s) 64, 242, 498, 817, 1233
Bst6I CTCTTC 4 cut(s) 364, 679, 924, 1139
BstBI TTCGAA 1 cut(s) 1342
BstC8I GCNNGC 2 cut(s) 156, 739
BstDEI CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1008, 1139, 1257
BstF5I GGATG 2 cut(s) 10, 1196
BstFNI CGCG 1 cut(s) 38
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 6 cut(s) 135, 369, 790, 1077, 1324, 1328
BstMAI GTCTC 3 cut(s) 823, 1054, 1076
BstMBI GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
BstMWI GCNNNNNNNGC 1 cut(s) 633
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 2 cut(s) 158, 423
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 2 cut(s) 241, 763
BstSLI GKGCMC 2 cut(s) 576, 1308
BstUI CGCG 1 cut(s) 38
BstV1I GCAGC 1 cut(s) 777
Bsu15I ATCGAT 1 cut(s) 1324
Bsu36I CCTNAGG 2 cut(s) 577, 1139
BsuRI GGCC 2 cut(s) 574, 741
BsuTUI ATCGAT 1 cut(s) 1324
BtsCI GGATG 2 cut(s) 10, 1196
BtsIMutI CAGTG 4 cut(s) 69, 376, 503, 813
Cac8I GCNNGC 2 cut(s) 156, 739
CciI TCATGA 1 cut(s) 1288
CfoI GCGC 1 cut(s) 387
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 4 cut(s) 572, 573, 988, 1229
ClaI ATCGAT 1 cut(s) 1324
CseI GACGC 1 cut(s) 768
Csp6I GTAC 1 cut(s) 96
CspCI CAANNNNNGTGG 2 cut(s) 1207, 1242
CviAII CATG 8 cut(s) 138, 155, 295, 420, 992, 1078, 1266, 1289
CviQI GTAC 1 cut(s) 96
DdeI CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1008, 1139, 1257
DpnI GATC 6 cut(s) 134, 368, 789, 1076, 1323, 1327
DpnII GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
DraI TTTAAA 1 cut(s) 102
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 364, 679, 924, 1139
EarI CTCTTC 4 cut(s) 364, 679, 924, 1139
EciI GGCGGA 1 cut(s) 675
Ecl136II GAGCTC 1 cut(s) 357
Eco24I GRGCYC 3 cut(s) 359, 554, 576
Eco31I GGTCTC 1 cut(s) 1054
Eco47I GGWCC 2 cut(s) 988, 1229
Eco47III AGCGCT 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 357
Eco81I CCTNAGG 2 cut(s) 577, 1139
Eco88I CYCGRG 1 cut(s) 822
EcoICRI GAGCTC 1 cut(s) 357
EcoO109I RGGNCCY 1 cut(s) 573
EcoRII CCWGG 1 cut(s) 655
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 3 cut(s) 359, 554, 576
FaeI CATG 8 cut(s) 141, 158, 298, 423, 995, 1081, 1269, 1292
FalI AAGNNNNNCTT 2 cut(s) 739, 771
FaqI GGGAC 1 cut(s) 54
FatI CATG 8 cut(s) 137, 154, 294, 419, 991, 1077, 1265, 1288
FauI CCCGC 2 cut(s) 31, 69
FbaI TGATCA 2 cut(s) 132, 787
Fnu4HI GCNGC 1 cut(s) 766
FokI GGATG 2 cut(s) 17, 1183
FriOI GRGCYC 3 cut(s) 359, 554, 576
Fsp4HI GCNGC 1 cut(s) 766
FspBI CTAG 4 cut(s) 432, 689, 1128, 1298
GlaI GCGC 1 cut(s) 386
GluI GCNGC 1 cut(s) 766
GsuI CTGGAG 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 2 cut(s) 574, 741
HapII CCGG 1 cut(s) 738
HgaI GACGC 1 cut(s) 768
HhaI GCGC 1 cut(s) 387
Hin1II CATG 8 cut(s) 141, 158, 298, 423, 995, 1081, 1269, 1292
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HindIII AAGCTT 1 cut(s) 1122
HpaII CCGG 1 cut(s) 738
HphI GGTGA 3 cut(s) 554, 645, 672
Hpy166II GTNNAC 1 cut(s) 1229
Hpy188I TCNGA 3 cut(s) 27, 787, 928
Hpy8I GTNNAC 1 cut(s) 1229
Hpy99I CGWCG 1 cut(s) 288
HpyAV CCTTC 4 cut(s) 86, 498, 841, 1263
HpyCH4III ACNGT 5 cut(s) 64, 242, 498, 817, 1233
HpyF10VI GCNNNNNNNGC 1 cut(s) 633
HpyF3I CTNAG 8 cut(s) 24, 263, 268, 577, 775, 1008, 1139, 1257
Hsp92II CATG 8 cut(s) 141, 158, 298, 423, 995, 1081, 1269, 1292
HspAI GCGC 1 cut(s) 385
KroI GCCGGC 1 cut(s) 737
KroNI GCCGGC 1 cut(s) 739
Ksp22I TGATCA 2 cut(s) 132, 787
Kzo9I GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
LguI GCTCTTC 2 cut(s) 364, 1139
LmnI GCTCC 4 cut(s) 73, 522, 1241, 1261
Lsp1109I GCAGC 1 cut(s) 777
LweI GCATC 4 cut(s) 10, 675, 724, 1282
MaeI CTAG 4 cut(s) 432, 689, 1128, 1298
MalI GATC 6 cut(s) 134, 368, 789, 1076, 1323, 1327
MbiI CCGCTC 1 cut(s) 76
MboI GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
MboII GAAGA 7 cut(s) 351, 612, 666, 786, 941, 1156, 1331
MhlI GDGCHC 5 cut(s) 359, 554, 576, 1238, 1308
MlyI GAGTC 4 cut(s) 644, 932, 934, 1019
Mph1103I ATGCAT 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 737
MroXI GAANNNNTTC 2 cut(s) 279, 303
MseI TTAA 4 cut(s) 101, 174, 545, 708
MslI CAYNNNNRTG 3 cut(s) 312, 723, 1359
MspA1I CMGCKG 1 cut(s) 344
MspI CCGG 1 cut(s) 738
MspR9I CCNGG 1 cut(s) 657
Mva1269I GAATGC 2 cut(s) 317, 834
MvaI CCWGG 1 cut(s) 657
MvnI CGCG 1 cut(s) 38
MwoI GCNNNNNNNGC 1 cut(s) 633
NaeI GCCGGC 1 cut(s) 739
NdeII GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
NgoMIV GCCGGC 1 cut(s) 737
NlaIII CATG 8 cut(s) 141, 158, 298, 423, 995, 1081, 1269, 1292
NlaIV GGNNCC 3 cut(s) 211, 574, 1263
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 2 cut(s) 158, 423
NspV TTCGAA 1 cut(s) 1342
PaeI GCATGC 1 cut(s) 158
PagI TCATGA 1 cut(s) 1288
PciI ACATGT 1 cut(s) 419
PciSI GCTCTTC 2 cut(s) 364, 1139
PctI GAATGC 2 cut(s) 317, 834
PdiI GCCGGC 1 cut(s) 739
PdmI GAANNNNTTC 2 cut(s) 279, 303
PfeI GAWTC 7 cut(s) 50, 80, 247, 304, 435, 856, 997
PflMI CCANNNNNTGG 1 cut(s) 912
PkrI GCNGC 1 cut(s) 767
PleI GAGTC 4 cut(s) 644, 931, 934, 1018
PpsI GAGTC 4 cut(s) 644, 931, 934, 1018
PscI ACATGT 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 3 cut(s) 211, 574, 1263
PspOMI GGGCCC 1 cut(s) 572
PspPI GGNCC 4 cut(s) 572, 573, 988, 1229
PstI CTGCAG 1 cut(s) 767
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 1 cut(s) 97
RsaNI GTAC 1 cut(s) 96
RseI CAYNNNNRTG 3 cut(s) 312, 723, 1359
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 2 cut(s) 364, 1139
SaqAI TTAA 4 cut(s) 101, 174, 545, 708
SatI GCNGC 1 cut(s) 766
Sau3AI GATC 6 cut(s) 132, 366, 787, 1074, 1321, 1325
Sau96I GGNCC 4 cut(s) 572, 573, 988, 1229
SchI GAGTC 4 cut(s) 644, 932, 934, 1019
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 5 cut(s) 359, 554, 576, 1238, 1308
SfaNI GCATC 4 cut(s) 10, 675, 724, 1282
SfcI CTRYAG 2 cut(s) 241, 763
SfuI TTCGAA 1 cut(s) 1342
SinI GGWCC 2 cut(s) 988, 1229
SmiMI CAYNNNNRTG 3 cut(s) 312, 723, 1359
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SphI GCATGC 1 cut(s) 158
SsiI CCGC 3 cut(s) 38, 76, 660
SspMI CTAG 4 cut(s) 432, 689, 1128, 1298
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 655
TaaI ACNGT 5 cut(s) 64, 242, 498, 817, 1233
TaqI TCGA 8 cut(s) 107, 331, 353, 629, 921, 1160, 1324, 1342
TfiI GAWTC 7 cut(s) 50, 80, 247, 304, 435, 856, 997
Tru1I TTAA 4 cut(s) 101, 174, 545, 708
Tru9I TTAA 4 cut(s) 101, 174, 545, 708
TscAI CASTG 4 cut(s) 69, 376, 503, 820
TseI GCWGC 1 cut(s) 765
TspDTI ATGAA 8 cut(s) 42, 288, 296, 666, 741, 897, 953, 1046
TspRI CASTG 4 cut(s) 69, 376, 503, 820
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 2 cut(s) 988, 1229
XapI RAATTY 6 cut(s) 11, 103, 278, 728, 1088, 1337
XceI RCATGY 2 cut(s) 158, 423
XmnI GAANNNNTTC 2 cut(s) 279, 303
XspI CTAG 4 cut(s) 432, 689, 1128, 1298
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.