MD16G1225600.v1.1

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0004960
Physical Location & Seq
Reverse (-)
22772674 .. 22776766
4093 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1225600.v1.1.491

Sequence Viewer

Length: 1932 bp
ATGGATGATAAGTTTGTTGATGCTCAGAAAGACCACCAGGGGCAAGAGGATTCGTTGCATAGATTGAACGAGGAGCGGGAATTATCAGGAGAAGGTACTTTAAGTTCTTCTGGCTCAAAATTGAAAAGGTTGGTCACATGGTCAAATCCACAGCATGCACTAATTTCAAATGTTAAGCGTTTGCAAATTAGTAAATGTTGCATAAAAGGTTCCTTGTTTCAAAGTGGTATTGGCTTGGACAGTAGTATTCCAAAGCACATACTGAGCTTAGATGAAAAATTTCGTCAAAAATGCATGGAATTGATTCATATTAGTGCATCCAAAGTAGCTCATTGTAATACAGCTGTGAAATTGAGCTCTTCAACGATCAGTGCTTTGCCCGAGTGCTTGAATGTGGCTGAAAGTAGAAGTGGAGATACGTGTAGTTCTGCTAGATTCGTGATTGAATGTCCATTGGCAACTGGTGGTGGGAGTGTAGTAATAAGCCCTGCCGGACAGTGGATTGTGGGTAGAGTTATGGGGAGCAAGAGTATGACGAATATATTAAAGAGCCCTTTGTTTCACCAATTTTGGCCCTTGGGTAGCAATACAGACTTAAGAAGAATAGACTGCAATAATGACAAAGGCTCGATATGCTATGATTTCACAGACTCACCTGGAGGATTTAGTTTCTCTTCTTCACCGATGCTAGAAAAGGAAACACCAATTAAGGGAAGCCATAAAAGTGAATCTAATGCCGGCCACAAAAGACTTATTTCTATCTGCAGCACAAACTCAGCATTGTCCGATCAATCTTCTGCTTCCACTCTTGCCACTGTTTCTCGGGGAATGCTTCAATGCACGTGGAAGGGAGGGAATCCTCATTTTGTTTTCTCCACAGATGAACAAAGGGAGGTCTATGTAGCCAACATGTGGAAAGTCGAGTCTGAAGAGGATAAGACTCTAGACTATATTTATTTGTTTCATTCAGGAAAGGGTGGGCAAAAGGACCATGAGATTCGTGATGGTGAGTCACACCTTGTCGGTAAGATGAAGGTCAATAATTTGGTCTCCCTCTGCCCAAGCAATTCAAAGATCATGGAGACTGAATTTATTTTGTTTGGTGGTGGTATTGAGATGCACACTTGTAGCCATAACCTCAGGCAGAGCAAGGGGTTATCAAAAAAAGTGGTCGAGGCATTCAGGACAACTCACTCATCCAAACAAAGAACCATCTCCAAATTGAGTGGACCATGTTTCATAGTTGAAAATCCTTCTCAGGAGCCATGCCTTGATACAGGCAACAATCAAGATGCTCTACGTGTGCCCAATTTGTTAGAAGATCATCTCCCTCCAAATTTCGAATTGGCTTCCATTGTTGTGAAAGACCATCTCCCTGATGATCGAAAGGAGGAAGCTGGAGGTTGGGGATTGAAATTTCTAAAGAAAGTTGGGAATAAGAAAATCAGTAACTCAGTGGAGGCTTCAGCGCCTGTCGAATGTTGTCGAAATAATGGTGATTGCTCTACCAGTACGGATGTTCTAATCCCAGCAGGAATTCACGGTGGGCCAGGAACCAGAAATGGCGGTCCTTCAAGTCTTACCGAAAGATGGAGATCTGGTGGACAATGTGACTGTGGAGGTTGGGATCTGGGGTGCCCACTCACGGTACTCAAAACCAAATCAAGCAGAGAAGACATTTTGCCTCAATCCGACACTCAGGGGGAGTGCAAATCATTTGAATTAAATAGAACGGGTTCAGAGCATGGTCCTCCTGCATGGAGGATGTTGAATGTCCACGATGATTTGTATTTTGTTCATTTTCAACCATCTCTGTCAGTTCTGCAGTCTTTCGCAATTGCAGTAGCAATTATCCATACACAGAGTCCAGCCCTCCGACCCAAATGTACAGGCATTTTAAGTACCAAAATCCAGGGTTTGAAAATTATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

644

Amino Acids

70.16

Weight (kDa)

7.76

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 272 - 621 1e-125 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1635
AccB7I CCANNNNNTGG 1 cut(s) 912
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 2 cut(s) 76, 1566
AclWI GGATC 1 cut(s) 1635
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 5 cut(s) 278, 1088, 1336, 1415, 1536
AcuI CTGAAG 2 cut(s) 948, 1449
AcvI CACGTG 1 cut(s) 843
AfaI GTAC 5 cut(s) 97, 1513, 1650, 1888, 1903
AfiI CCNNNNNNNGG 5 cut(s) 498, 710, 912, 1590, 1645
AflII CTTAAG 1 cut(s) 595
AflIII ACRYGT 3 cut(s) 419, 909, 1300
AjnI CCWGG 4 cut(s) 36, 655, 1549, 1911
AluBI AGCT 5 cut(s) 267, 329, 344, 357, 1397
AluI AGCT 5 cut(s) 267, 329, 344, 357, 1397
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 2 cut(s) 1054, 1076
AlwI GGATC 1 cut(s) 1635
AlwNI CAGNNNCTG 1 cut(s) 1472
Ama87I CYCGRG 2 cut(s) 380, 822
AoxI GGCC 3 cut(s) 572, 739, 1547
ApeKI GCWGC 1 cut(s) 765
ApoI RAATTY 5 cut(s) 278, 1088, 1336, 1415, 1536
Asp700I GAANNNNTTC 3 cut(s) 279, 303, 1735
AspLEI GCGC 1 cut(s) 1471
AspS9I GGNCC 6 cut(s) 573, 988, 1229, 1547, 1568, 1748
AsuHPI GGTGA 5 cut(s) 554, 645, 672, 1019, 1508
AsuII TTCGAA 1 cut(s) 1341
AvaI CYCGRG 2 cut(s) 380, 822
AvaII GGWCC 4 cut(s) 988, 1229, 1568, 1748
AxyI CCTNAGG 1 cut(s) 1139
BaeGI GKGCMC 2 cut(s) 1308, 1640
BanI GGYRCC 1 cut(s) 1635
BanII GRGCYC 2 cut(s) 359, 554
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbrPI CACGTG 1 cut(s) 843
BbsI GAAGAC 1 cut(s) 1680
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 777
BccI CCATC 5 cut(s) 998, 1220, 1377, 1584, 1816
BciT130I CCWGG 4 cut(s) 38, 657, 1551, 1913
BcoDI GTCTC 2 cut(s) 1054, 1076
BfaI CTAG 3 cut(s) 432, 689, 944
BfmI CTRYAG 2 cut(s) 763, 1823
BfoI RGCGCY 1 cut(s) 1472
BfrI CTTAAG 1 cut(s) 595
BglII AGATCT 1 cut(s) 1595
BisI GCNGC 1 cut(s) 766
BlsI GCNGC 1 cut(s) 767
Bme1390I CCNGG 4 cut(s) 38, 657, 1551, 1913
Bme18I GGWCC 4 cut(s) 988, 1229, 1568, 1748
BmeT110I CYCGRG 2 cut(s) 380, 822
BmgT120I GGNCC 6 cut(s) 573, 988, 1229, 1547, 1568, 1748
BmiI GGNNCC 4 cut(s) 211, 1263, 1555, 1637
BmrFI CCNGG 4 cut(s) 38, 657, 1551, 1913
BmsI GCATC 5 cut(s) 10, 326, 675, 1107, 1282
BpiI GAAGAC 1 cut(s) 1680
BpmI CTGGAG 2 cut(s) 678, 1419
Bpu14I TTCGAA 1 cut(s) 1341
BsaAI YACGTR 3 cut(s) 420, 843, 1301
BsaBI GATNNNNATC 1 cut(s) 1594
BsaI GGTCTC 1 cut(s) 1054
BsaJI CCNNGG 3 cut(s) 37, 576, 1912
BsaXI ACNNNNNCTCC 4 cut(s) 514, 544, 1585, 1615
Bsc4I CCNNNNNNNGG 5 cut(s) 498, 710, 912, 1590, 1645
Bse118I RCCGGY 1 cut(s) 737
Bse1I ACTGG 2 cut(s) 466, 1509
Bse21I CCTNAGG 1 cut(s) 1139
Bse8I GATNNNNATC 1 cut(s) 1594
BseBI CCWGG 4 cut(s) 38, 657, 1551, 1913
BseDI CCNNGG 3 cut(s) 37, 576, 1912
BseGI GGATG 5 cut(s) 10, 317, 1196, 1522, 1770
BseJI GATNNNNATC 1 cut(s) 1594
BseLI CCNNNNNNNGG 5 cut(s) 498, 710, 912, 1590, 1645
BseMII CTCAG 7 cut(s) 38, 254, 789, 1153, 1271, 1467, 1712
BseNI ACTGG 2 cut(s) 466, 1509
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 2 cut(s) 1308, 1640
BseXI GCAGC 1 cut(s) 777
BseYI CCCAGC 1 cut(s) 1528
BshFI GGCC 3 cut(s) 574, 741, 1549
BshNI GGYRCC 1 cut(s) 1635
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 2 cut(s) 380, 822
BsiSI CCGG 2 cut(s) 492, 738
BslI CCNNNNNNNGG 5 cut(s) 498, 710, 912, 1590, 1645
BsmAI GTCTC 2 cut(s) 1054, 1076
BsmI GAATGC 2 cut(s) 834, 1178
BsnI GGCC 3 cut(s) 574, 741, 1549
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 2 cut(s) 380, 822
Bsp119I TTCGAA 1 cut(s) 1341
Bsp1286I GDGCHC 4 cut(s) 359, 554, 1308, 1640
Bsp1407I TGTACA 1 cut(s) 1886
Bsp143I GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BspACI CCGC 2 cut(s) 76, 1566
BspANI GGCC 3 cut(s) 574, 741, 1549
BspCNI CTCAG 7 cut(s) 37, 255, 788, 1152, 1270, 1466, 1711
BspLI GGNNCC 4 cut(s) 211, 1263, 1555, 1637
BspMAI CTGCAG 2 cut(s) 767, 1827
BspPI GGATC 1 cut(s) 1635
BspQI GCTCTTC 1 cut(s) 364
BspT104I TTCGAA 1 cut(s) 1341
BspT107I GGYRCC 1 cut(s) 1635
BspTI CTTAAG 1 cut(s) 595
BspTNI GGTCTC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 76
BsrFI RCCGGY 1 cut(s) 737
BsrGI TGTACA 1 cut(s) 1886
BsrI ACTGG 2 cut(s) 466, 1509
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 3 cut(s) 37, 576, 1912
BssMI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BssT1I CCWWGG 1 cut(s) 576
Bst2UI CCWGG 4 cut(s) 38, 657, 1551, 1913
Bst4CI ACNGT 6 cut(s) 242, 498, 817, 1544, 1616, 1648
Bst6I CTCTTC 3 cut(s) 364, 679, 924
BstAFI CTTAAG 1 cut(s) 595
BstAUI TGTACA 1 cut(s) 1886
BstBAI YACGTR 3 cut(s) 420, 843, 1301
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 2 cut(s) 156, 739
BstDEI CTNAG 8 cut(s) 24, 263, 268, 775, 1139, 1257, 1453, 1698
BstF5I GGATG 5 cut(s) 10, 317, 1196, 1522, 1770
BstH2I RGCGCY 1 cut(s) 1472
BstHHI GCGC 1 cut(s) 1471
BstKTI GATC 7 cut(s) 369, 790, 1077, 1324, 1384, 1598, 1630
BstMAI GTCTC 2 cut(s) 1054, 1076
BstMBI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
BstMWI GCNNNNNNNGC 1 cut(s) 633
BstNI CCWGG 4 cut(s) 38, 657, 1551, 1913
BstNSI RCATGY 2 cut(s) 158, 913
BstSCI CCNGG 4 cut(s) 36, 655, 1549, 1911
BstSFI CTRYAG 2 cut(s) 763, 1823
BstSLI GKGCMC 2 cut(s) 1308, 1640
BstV1I GCAGC 1 cut(s) 777
BstV2I GAAGAC 1 cut(s) 1680
BstX2I RGATCY 2 cut(s) 1595, 1627
BstYI RGATCY 2 cut(s) 1595, 1627
Bsu36I CCTNAGG 1 cut(s) 1139
BsuRI GGCC 3 cut(s) 574, 741, 1549
BtsCI GGATG 5 cut(s) 10, 317, 1196, 1522, 1770
BtsIMutI CAGTG 4 cut(s) 376, 503, 813, 1461
Cac8I GCNNGC 2 cut(s) 156, 739
CaiI CAGNNNCTG 1 cut(s) 1472
CfoI GCGC 1 cut(s) 1471
Cfr10I RCCGGY 1 cut(s) 737
Cfr13I GGNCC 6 cut(s) 573, 988, 1229, 1547, 1568, 1748
Csp6I GTAC 5 cut(s) 96, 1512, 1649, 1887, 1902
CspCI CAANNNNNGTGG 6 cut(s) 824, 859, 1149, 1184, 1207, 1242
CviQI GTAC 5 cut(s) 96, 1512, 1649, 1887, 1902
DdeI CTNAG 8 cut(s) 24, 263, 268, 775, 1139, 1257, 1453, 1698
DpnI GATC 7 cut(s) 368, 789, 1076, 1323, 1383, 1597, 1629
DpnII GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 3 cut(s) 364, 679, 924
EarI CTCTTC 3 cut(s) 364, 679, 924
Ecl136II GAGCTC 1 cut(s) 357
Eco130I CCWWGG 1 cut(s) 576
Eco24I GRGCYC 2 cut(s) 359, 554
Eco31I GGTCTC 1 cut(s) 1054
Eco47I GGWCC 4 cut(s) 988, 1229, 1568, 1748
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 2 cut(s) 948, 1449
Eco72I CACGTG 1 cut(s) 843
Eco81I CCTNAGG 1 cut(s) 1139
Eco88I CYCGRG 2 cut(s) 380, 822
EcoICRI GAGCTC 1 cut(s) 357
EcoRI GAATTC 1 cut(s) 1536
EcoRII CCWGG 4 cut(s) 36, 655, 1549, 1911
EcoT14I CCWWGG 1 cut(s) 576
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 359, 554
ErhI CCWWGG 1 cut(s) 576
FauI CCCGC 1 cut(s) 69
Fnu4HI GCNGC 1 cut(s) 766
FokI GGATG 5 cut(s) 17, 304, 1183, 1529, 1777
FriOI GRGCYC 2 cut(s) 359, 554
Fsp4HI GCNGC 1 cut(s) 766
FspBI CTAG 3 cut(s) 432, 689, 944
GlaI GCGC 1 cut(s) 1470
GluI GCNGC 1 cut(s) 766
GsaI CCCAGC 1 cut(s) 1532
GsuI CTGGAG 2 cut(s) 678, 1419
HaeII RGCGCY 1 cut(s) 1472
HaeIII GGCC 3 cut(s) 574, 741, 1549
HapII CCGG 2 cut(s) 492, 738
HhaI GCGC 1 cut(s) 1471
Hin6I GCGC 1 cut(s) 1469
HinP1I GCGC 1 cut(s) 1469
HpaII CCGG 2 cut(s) 492, 738
HphI GGTGA 5 cut(s) 554, 645, 672, 1019, 1508
Hpy166II GTNNAC 3 cut(s) 1229, 1604, 1777
Hpy188I TCNGA 6 cut(s) 27, 787, 928, 1693, 1741, 1877
Hpy188III TCNNGA 8 cut(s) 87, 439, 944, 969, 1001, 1183, 1259, 1289
Hpy8I GTNNAC 3 cut(s) 1229, 1604, 1777
HpyAV CCTTC 5 cut(s) 86, 841, 1027, 1263, 1581
HpyCH4III ACNGT 6 cut(s) 242, 498, 817, 1544, 1616, 1648
HpyCH4IV ACGT 3 cut(s) 419, 842, 1300
HpyF10VI GCNNNNNNNGC 1 cut(s) 633
HpyF3I CTNAG 8 cut(s) 24, 263, 268, 775, 1139, 1257, 1453, 1698
HpySE526I ACGT 3 cut(s) 419, 842, 1300
HspAI GCGC 1 cut(s) 1469
KroI GCCGGC 1 cut(s) 737
KroNI GCCGGC 1 cut(s) 739
Kzo9I GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
LguI GCTCTTC 1 cut(s) 364
LmnI GCTCC 3 cut(s) 73, 522, 1261
Lsp1109I GCAGC 1 cut(s) 777
LweI GCATC 5 cut(s) 10, 326, 675, 1107, 1282
MaeI CTAG 3 cut(s) 432, 689, 944
MaeII ACGT 3 cut(s) 419, 842, 1300
MaeIII GTNAC 4 cut(s) 133, 1011, 1448, 1610
MalI GATC 7 cut(s) 368, 789, 1076, 1323, 1383, 1597, 1629
MbiI CCGCTC 1 cut(s) 76
MboI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
MboII GAAGA 9 cut(s) 99, 351, 612, 666, 669, 786, 941, 1331, 1685
MfeI CAATTG 1 cut(s) 1836
MflI RGATCY 2 cut(s) 1595, 1627
MhlI GDGCHC 4 cut(s) 359, 554, 1308, 1640
MlyI GAGTC 5 cut(s) 644, 932, 934, 1019, 1873
MmeI TCCRAC 2 cut(s) 1716, 1900
Mph1103I ATGCAT 1 cut(s) 296
MroNI GCCGGC 1 cut(s) 737
MroXI GAANNNNTTC 3 cut(s) 279, 303, 1735
MseI TTAA 7 cut(s) 101, 174, 545, 596, 708, 1724, 1898
MslI CAYNNNNRTG 3 cut(s) 312, 723, 1358
MspA1I CMGCKG 1 cut(s) 344
MspCI CTTAAG 1 cut(s) 595
MspI CCGG 2 cut(s) 492, 738
MspR9I CCNGG 4 cut(s) 38, 657, 1551, 1913
MunI CAATTG 1 cut(s) 1836
Mva1269I GAATGC 2 cut(s) 834, 1178
MvaI CCWGG 4 cut(s) 38, 657, 1551, 1913
MwoI GCNNNNNNNGC 1 cut(s) 633
NaeI GCCGGC 1 cut(s) 739
NdeII GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
NgoMIV GCCGGC 1 cut(s) 737
NlaIV GGNNCC 4 cut(s) 211, 1263, 1555, 1637
NmuCI GTSAC 3 cut(s) 133, 1011, 1610
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 2 cut(s) 158, 913
NspV TTCGAA 1 cut(s) 1341
PaeI GCATGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 909
PciSI GCTCTTC 1 cut(s) 364
PctI GAATGC 2 cut(s) 834, 1178
PdiI GCCGGC 1 cut(s) 739
PdmI GAANNNNTTC 3 cut(s) 279, 303, 1735
PfeI GAWTC 6 cut(s) 50, 304, 435, 728, 856, 997
PflMI CCANNNNNTGG 1 cut(s) 912
PkrI GCNGC 1 cut(s) 767
PleI GAGTC 5 cut(s) 644, 931, 934, 1018, 1872
PmaCI CACGTG 1 cut(s) 843
PmlI CACGTG 1 cut(s) 843
PpsI GAGTC 5 cut(s) 644, 931, 934, 1018, 1872
Ppu21I YACGTR 3 cut(s) 420, 843, 1301
PscI ACATGT 1 cut(s) 909
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 4 cut(s) 36, 655, 1549, 1911
PspCI CACGTG 1 cut(s) 843
PspFI CCCAGC 1 cut(s) 1528
PspGI CCWGG 4 cut(s) 36, 655, 1549, 1911
PspN4I GGNNCC 4 cut(s) 211, 1263, 1555, 1637
PspPI GGNCC 6 cut(s) 573, 988, 1229, 1547, 1568, 1748
PsrI GAACNNNNNNTAC 2 cut(s) 88, 120
PstI CTGCAG 2 cut(s) 767, 1827
PstNI CAGNNNCTG 1 cut(s) 1472
PsuI RGATCY 2 cut(s) 1595, 1627
PvuII CAGCTG 1 cut(s) 344
RsaI GTAC 5 cut(s) 97, 1513, 1650, 1888, 1903
RsaNI GTAC 5 cut(s) 96, 1512, 1649, 1887, 1902
RseI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 1 cut(s) 364
SaqAI TTAA 7 cut(s) 101, 174, 545, 596, 708, 1724, 1898
SatI GCNGC 1 cut(s) 766
Sau3AI GATC 7 cut(s) 366, 787, 1074, 1321, 1381, 1595, 1627
Sau96I GGNCC 6 cut(s) 573, 988, 1229, 1547, 1568, 1748
SchI GAGTC 5 cut(s) 644, 932, 934, 1019, 1873
ScrFI CCNGG 4 cut(s) 38, 657, 1551, 1913
SduI GDGCHC 4 cut(s) 359, 554, 1308, 1640
SfaNI GCATC 5 cut(s) 10, 326, 675, 1107, 1282
SfcI CTRYAG 2 cut(s) 763, 1823
SfuI TTCGAA 1 cut(s) 1341
SinI GGWCC 4 cut(s) 988, 1229, 1568, 1748
SmiMI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SphI GCATGC 1 cut(s) 158
SsiI CCGC 2 cut(s) 76, 1566
SspMI CTAG 3 cut(s) 432, 689, 944
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 4 cut(s) 36, 655, 1549, 1911
StyI CCWWGG 1 cut(s) 576
TaaI ACNGT 6 cut(s) 242, 498, 817, 1544, 1616, 1648
TaiI ACGT 3 cut(s) 422, 845, 1303
TaqI TCGA 7 cut(s) 629, 921, 1173, 1341, 1384, 1476, 1486
TatI WGTACW 1 cut(s) 1886
TfiI GAWTC 6 cut(s) 50, 304, 435, 728, 856, 997
Tru1I TTAA 7 cut(s) 101, 174, 545, 596, 708, 1724, 1898
Tru9I TTAA 7 cut(s) 101, 174, 545, 596, 708, 1724, 1898
TscAI CASTG 4 cut(s) 376, 503, 820, 1461
TseFI GTSAC 3 cut(s) 133, 1011, 1610
TseI GCWGC 1 cut(s) 765
Tsp45I GTSAC 3 cut(s) 133, 1011, 1610
TspDTI ATGAA 7 cut(s) 288, 296, 897, 953, 1046, 1228, 1787
TspGWI ACGGA 1 cut(s) 1529
TspRI CASTG 4 cut(s) 376, 503, 820, 1461
Van91I CCANNNNNTGG 1 cut(s) 912
Vha464I CTTAAG 1 cut(s) 595
VpaK11BI GGWCC 4 cut(s) 988, 1229, 1568, 1748
XapI RAATTY 5 cut(s) 278, 1088, 1336, 1415, 1536
XbaI TCTAGA 1 cut(s) 943
XceI RCATGY 2 cut(s) 158, 913
XmnI GAANNNNTTC 3 cut(s) 279, 303, 1735
XspI CTAG 3 cut(s) 432, 689, 944
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.