pycom16g18870

Domain of unknown function (DUF3527)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0004960
Physical Location & Seq
Reverse (-)
15636116 .. 15637495
1380 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g18870.1

Sequence Viewer

Length: 1380 bp
ATGGATGATAAGTTTGTTGATGCTCAGAAAGACCAGCAGGGGCAAGATGATTCGTTGCGTAGATTGAGCGAGGAGCGGGAATTATCAGGAGAAGGTACTTTAAGTTCTTCTGGCTCAAAATTGAAAAGGTTGGTCACATGGTCAAATCCACAGCATGCACTAATTTCAAATGTTAAGCGTTTGCAAATTAGTAAATGTTGCATAAAAGGTTCCTTGTCTCAAAGTGGTATTGGCTTGGACAGTAATATTCCAAAGCACATACTGAGCTTAGATGAAAAATTTCGTCAAAAATGCATGGAATTGATTCATATTAGTGCATCCAAAGTAGCTCGTTGTAATACAGCTGTGAAATTGAGCTCTTCAAAGATCAGTGCTTTGCCCGAGCGCTTGAATGTGGCTGAAAGTAGAAGTGGAGATACGTGTAGTTCTGCTAGATTCGTGATTGAATGTCCATTGGCAGCTGGAGGTGGGAGTGTAGTAATAAGCCCTGCCGGACAGTGGATTGTGGGTAGAGTTATGGGGAGCAAGAGTATTATGAATACATTAAAGAGCCCTTTGTTTCACCAATTTTGGCCTTTGGGTGGCAATACAGACTTGAGAAGAATAGACTGCAATAATGACAGAGGCTCGATATGCTATGATTTCACAGACTCACCTGGCGGATTTAGTTTCTCTTCTTCACCAATGCTAGAAAAGGAAACACCAATTCAGGGAAGCCATAAAAGTGAATCTAATGCTGGCCACAAAAGACTTATTTCTATCTGCAGCACAAACTCAGCATTGTCTGATCAATCTTCTGCTTCCACCCTTGCCACTGTTTCTCGGGGAATGCTTCAATGCACGTGGAAGGGAGGGAATCCTCATTTTGTTTTCTCCACAGATGAACAAAGGGAGGTCTATGTAGCCAACATGTGGAAGGTCGAGTCTGAAGAGGATAAGACTCTGGACTATATATATTTGTTTCATTCAGGAAAGGGTGGGCAAAAGGACCATGAGATTCGTGATAGTGAGTCACACCTTGTCGGTAAGATGAAGGTCAATAATTTGGTCTCCCTCTGCCCAAGCAATTCAAAGATCATGGAGACTGAATTTAATTTGTTTGGTGGCGGTATTGAGATGTACAATTCTAGCCATAACCTCAGGAAGAGCAAGGGGTTATCAAAAAAAGTGGTCGAAGCATTCAGGACAACTCACTCATCCAAACAAAGAACCATCTCCAAATTGAGTGGACCATGTTTCATAGTTGAAAATCCTTCTCGGGAGCCATGCCTTGATACAGGCAACAATCAAGATGCTCTACGTGTGCCCAATTTGGTAGAAGATCATCTCCCTCCAAATTTCGAATTGGCTTCCATTGTTGTGAAAGACCATCTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3527

Protein Analysis

460

Amino Acids

50.46

Weight (kDa)

8.47

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3527 PF12043 272 - 458 2.5e-51 Domain of unknown function (DUF3527)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1378
AccB7I CCANNNNNTGG 1 cut(s) 912
AccBSI CCGCTC 1 cut(s) 76
AciI CCGC 3 cut(s) 76, 660, 1107
AcoI YGGCCR 1 cut(s) 739
AcsI RAATTY 3 cut(s) 278, 1088, 1336
AcuI CTGAAG 1 cut(s) 948
AcvI CACGTG 1 cut(s) 843
AfaI GTAC 2 cut(s) 97, 1121
AfeI AGCGCT 1 cut(s) 386
AfiI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
AflIII ACRYGT 3 cut(s) 419, 909, 1300
AgsI TTSAA 8 cut(s) 124, 168, 363, 391, 446, 836, 1071, 1247
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 5 cut(s) 267, 329, 344, 357, 461
AluI AGCT 5 cut(s) 267, 329, 344, 357, 461
Alw21I GWGCWC 1 cut(s) 359
Alw26I GTCTC 3 cut(s) 222, 1054, 1076
Ama87I CYCGRG 3 cut(s) 380, 822, 1257
Aor51HI AGCGCT 1 cut(s) 386
AoxI GGCC 2 cut(s) 572, 739
ApeKI GCWGC 2 cut(s) 458, 765
ApoI RAATTY 3 cut(s) 278, 1088, 1336
Asp700I GAANNNNTTC 2 cut(s) 279, 303
AspLEI GCGC 1 cut(s) 387
AspS9I GGNCC 2 cut(s) 988, 1229
AsuHPI GGTGA 3 cut(s) 554, 645, 672
AsuII TTCGAA 1 cut(s) 1341
AvaI CYCGRG 3 cut(s) 380, 822, 1257
AvaII GGWCC 2 cut(s) 988, 1229
AxyI CCTNAGG 1 cut(s) 1139
BaeGI GKGCMC 1 cut(s) 1308
BalI TGGCCA 1 cut(s) 741
BanII GRGCYC 2 cut(s) 359, 554
BarI GAAGNNNNNNTAC 2 cut(s) 400, 432
BbrPI CACGTG 1 cut(s) 843
Bbv12I GWGCWC 1 cut(s) 359
BbvI GCAGC 2 cut(s) 470, 777
BccI CCATC 2 cut(s) 1220, 1377
BciT130I CCWGG 1 cut(s) 657
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 3 cut(s) 222, 1054, 1076
BfaI CTAG 3 cut(s) 432, 689, 1128
BfmI CTRYAG 1 cut(s) 763
BfoI RGCGCY 1 cut(s) 388
BisI GCNGC 2 cut(s) 459, 766
BlsI GCNGC 2 cut(s) 460, 767
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 2 cut(s) 988, 1229
BmeT110I CYCGRG 3 cut(s) 380, 822, 1257
BmgT120I GGNCC 2 cut(s) 988, 1229
BmiI GGNNCC 2 cut(s) 211, 1263
BmrFI CCNGG 1 cut(s) 657
BmsI GCATC 3 cut(s) 10, 326, 1282
BpmI CTGGAG 1 cut(s) 483
Bpu14I TTCGAA 1 cut(s) 1341
BpuEI CTTGAG 1 cut(s) 616
BsaAI YACGTR 3 cut(s) 420, 843, 1301
BsaI GGTCTC 1 cut(s) 1054
BsaXI ACNNNNNCTCC 2 cut(s) 514, 544
Bsc4I CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
Bse21I CCTNAGG 1 cut(s) 1139
BseBI CCWGG 1 cut(s) 657
BseGI GGATG 3 cut(s) 10, 317, 1196
BseLI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
BseMII CTCAG 4 cut(s) 38, 254, 789, 1153
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 1308
BseXI GCAGC 2 cut(s) 470, 777
BshFI GGCC 2 cut(s) 574, 741
BsiHKAI GWGCWC 1 cut(s) 359
BsiHKCI CYCGRG 3 cut(s) 380, 822, 1257
BsiSI CCGG 1 cut(s) 492
BslI CCNNNNNNNGG 4 cut(s) 498, 581, 710, 912
BsmAI GTCTC 3 cut(s) 222, 1054, 1076
BsmI GAATGC 2 cut(s) 834, 1178
BsnI GGCC 2 cut(s) 574, 741
Bso31I GGTCTC 1 cut(s) 1054
BsoBI CYCGRG 3 cut(s) 380, 822, 1257
Bsp119I TTCGAA 1 cut(s) 1341
Bsp1286I GDGCHC 3 cut(s) 359, 554, 1308
Bsp1407I TGTACA 1 cut(s) 1119
Bsp143I GATC 4 cut(s) 366, 787, 1074, 1321
BspACI CCGC 3 cut(s) 76, 660, 1107
BspANI GGCC 2 cut(s) 574, 741
BspCNI CTCAG 4 cut(s) 37, 255, 788, 1152
BspLI GGNNCC 2 cut(s) 211, 1263
BspMAI CTGCAG 1 cut(s) 767
BspQI GCTCTTC 2 cut(s) 364, 1139
BspT104I TTCGAA 1 cut(s) 1341
BspTNI GGTCTC 1 cut(s) 1054
BsrBI CCGCTC 1 cut(s) 76
BsrGI TGTACA 1 cut(s) 1119
BssMI GATC 4 cut(s) 366, 787, 1074, 1321
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 3 cut(s) 242, 498, 817
Bst6I CTCTTC 4 cut(s) 364, 679, 924, 1139
BstAUI TGTACA 1 cut(s) 1119
BstBAI YACGTR 3 cut(s) 420, 843, 1301
BstBI TTCGAA 1 cut(s) 1341
BstC8I GCNNGC 2 cut(s) 156, 739
BstDEI CTNAG 5 cut(s) 24, 263, 268, 775, 1139
BstF5I GGATG 3 cut(s) 10, 317, 1196
BstH2I RGCGCY 1 cut(s) 388
BstHHI GCGC 1 cut(s) 387
BstKTI GATC 4 cut(s) 369, 790, 1077, 1324
BstMAI GTCTC 3 cut(s) 222, 1054, 1076
BstMBI GATC 4 cut(s) 366, 787, 1074, 1321
BstMWI GCNNNNNNNGC 1 cut(s) 633
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 2 cut(s) 158, 913
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 1 cut(s) 763
BstSLI GKGCMC 1 cut(s) 1308
BstV1I GCAGC 2 cut(s) 470, 777
Bsu36I CCTNAGG 1 cut(s) 1139
BsuRI GGCC 2 cut(s) 574, 741
BtsCI GGATG 3 cut(s) 10, 317, 1196
BtsIMutI CAGTG 3 cut(s) 376, 503, 813
Cac8I GCNNGC 2 cut(s) 156, 739
CfoI GCGC 1 cut(s) 387
Cfr13I GGNCC 2 cut(s) 988, 1229
Csp6I GTAC 2 cut(s) 96, 1120
CspCI CAANNNNNGTGG 6 cut(s) 824, 859, 1149, 1184, 1207, 1242
CviAII CATG 8 cut(s) 138, 155, 295, 910, 992, 1078, 1233, 1266
CviQI GTAC 2 cut(s) 96, 1120
DdeI CTNAG 5 cut(s) 24, 263, 268, 775, 1139
DpnI GATC 4 cut(s) 368, 789, 1076, 1323
DpnII GATC 4 cut(s) 366, 787, 1074, 1321
EaeI YGGCCR 1 cut(s) 739
Eam1104I CTCTTC 4 cut(s) 364, 679, 924, 1139
EarI CTCTTC 4 cut(s) 364, 679, 924, 1139
EciI GGCGGA 1 cut(s) 675
Ecl136II GAGCTC 1 cut(s) 357
Eco24I GRGCYC 2 cut(s) 359, 554
Eco31I GGTCTC 1 cut(s) 1054
Eco47I GGWCC 2 cut(s) 988, 1229
Eco47III AGCGCT 1 cut(s) 386
Eco53kI GAGCTC 1 cut(s) 357
Eco57I CTGAAG 1 cut(s) 948
Eco72I CACGTG 1 cut(s) 843
Eco81I CCTNAGG 1 cut(s) 1139
Eco88I CYCGRG 3 cut(s) 380, 822, 1257
EcoICRI GAGCTC 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 655
EcoT22I ATGCAT 1 cut(s) 296
EcoT38I GRGCYC 2 cut(s) 359, 554
FaeI CATG 8 cut(s) 141, 158, 298, 913, 995, 1081, 1236, 1269
FatI CATG 8 cut(s) 137, 154, 294, 909, 991, 1077, 1232, 1265
FauI CCCGC 1 cut(s) 69
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 2 cut(s) 459, 766
FokI GGATG 3 cut(s) 17, 304, 1183
FriOI GRGCYC 2 cut(s) 359, 554
Fsp4HI GCNGC 2 cut(s) 459, 766
FspBI CTAG 3 cut(s) 432, 689, 1128
GlaI GCGC 1 cut(s) 386
GluI GCNGC 2 cut(s) 459, 766
GsuI CTGGAG 1 cut(s) 483
HaeII RGCGCY 1 cut(s) 388
HaeIII GGCC 2 cut(s) 574, 741
HapII CCGG 1 cut(s) 492
HhaI GCGC 1 cut(s) 387
Hin1II CATG 8 cut(s) 141, 158, 298, 913, 995, 1081, 1236, 1269
Hin6I GCGC 1 cut(s) 385
HinP1I GCGC 1 cut(s) 385
HpaII CCGG 1 cut(s) 492
HphI GGTGA 3 cut(s) 554, 645, 672
Hpy166II GTNNAC 1 cut(s) 1229
Hpy188I TCNGA 3 cut(s) 27, 787, 928
Hpy188III TCNNGA 9 cut(s) 87, 439, 944, 969, 1001, 1141, 1183, 1259, 1289
Hpy8I GTNNAC 1 cut(s) 1229
HpyAV CCTTC 5 cut(s) 86, 841, 910, 1027, 1263
HpyCH4III ACNGT 3 cut(s) 242, 498, 817
HpyCH4IV ACGT 3 cut(s) 419, 842, 1300
HpyCH4V TGCA 8 cut(s) 158, 184, 201, 294, 317, 612, 765, 840
HpyF10VI GCNNNNNNNGC 1 cut(s) 633
HpyF3I CTNAG 5 cut(s) 24, 263, 268, 775, 1139
HpySE526I ACGT 3 cut(s) 419, 842, 1300
Hsp92II CATG 8 cut(s) 141, 158, 298, 913, 995, 1081, 1236, 1269
HspAI GCGC 1 cut(s) 385
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 4 cut(s) 366, 787, 1074, 1321
LguI GCTCTTC 2 cut(s) 364, 1139
LmnI GCTCC 3 cut(s) 73, 522, 1261
Lsp1109I GCAGC 2 cut(s) 470, 777
LweI GCATC 3 cut(s) 10, 326, 1282
MaeI CTAG 3 cut(s) 432, 689, 1128
MaeII ACGT 3 cut(s) 419, 842, 1300
MaeIII GTNAC 2 cut(s) 133, 1011
MalI GATC 4 cut(s) 368, 789, 1076, 1323
MbiI CCGCTC 1 cut(s) 76
MboI GATC 4 cut(s) 366, 787, 1074, 1321
MboII GAAGA 9 cut(s) 99, 351, 612, 666, 669, 786, 941, 1156, 1331
MhlI GDGCHC 3 cut(s) 359, 554, 1308
MlsI TGGCCA 1 cut(s) 741
MluNI TGGCCA 1 cut(s) 741
MlyI GAGTC 4 cut(s) 644, 932, 934, 1019
Mox20I TGGCCA 1 cut(s) 741
Mph1103I ATGCAT 1 cut(s) 296
MroXI GAANNNNTTC 2 cut(s) 279, 303
MscI TGGCCA 1 cut(s) 741
MseI TTAA 4 cut(s) 101, 174, 545, 1092
MslI CAYNNNNRTG 3 cut(s) 312, 723, 1358
Msp20I TGGCCA 1 cut(s) 741
MspA1I CMGCKG 2 cut(s) 344, 461
MspI CCGG 1 cut(s) 492
MspR9I CCNGG 1 cut(s) 657
Mva1269I GAATGC 2 cut(s) 834, 1178
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 1 cut(s) 633
NdeII GATC 4 cut(s) 366, 787, 1074, 1321
NlaIII CATG 8 cut(s) 141, 158, 298, 913, 995, 1081, 1236, 1269
NlaIV GGNNCC 2 cut(s) 211, 1263
NmuCI GTSAC 2 cut(s) 133, 1011
NsiI ATGCAT 1 cut(s) 296
NspI RCATGY 2 cut(s) 158, 913
NspV TTCGAA 1 cut(s) 1341
PaeI GCATGC 1 cut(s) 158
PciI ACATGT 1 cut(s) 909
PciSI GCTCTTC 2 cut(s) 364, 1139
PctI GAATGC 2 cut(s) 834, 1178
PdmI GAANNNNTTC 2 cut(s) 279, 303
PfeI GAWTC 6 cut(s) 50, 304, 435, 728, 856, 997
PflMI CCANNNNNTGG 1 cut(s) 912
PkrI GCNGC 2 cut(s) 460, 767
PleI GAGTC 4 cut(s) 644, 931, 934, 1018
PmaCI CACGTG 1 cut(s) 843
PmlI CACGTG 1 cut(s) 843
PpsI GAGTC 4 cut(s) 644, 931, 934, 1018
Ppu21I YACGTR 3 cut(s) 420, 843, 1301
PscI ACATGT 1 cut(s) 909
PsiI TTATAA 1 cut(s) 1378
Psp124BI GAGCTC 1 cut(s) 359
Psp6I CCWGG 1 cut(s) 655
PspCI CACGTG 1 cut(s) 843
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 2 cut(s) 211, 1263
PspPI GGNCC 2 cut(s) 988, 1229
PsrI GAACNNNNNNTAC 2 cut(s) 88, 120
PstI CTGCAG 1 cut(s) 767
PvuII CAGCTG 2 cut(s) 344, 461
RsaI GTAC 2 cut(s) 97, 1121
RsaNI GTAC 2 cut(s) 96, 1120
RseI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SacI GAGCTC 1 cut(s) 359
SapI GCTCTTC 2 cut(s) 364, 1139
SaqAI TTAA 4 cut(s) 101, 174, 545, 1092
SatI GCNGC 2 cut(s) 459, 766
Sau3AI GATC 4 cut(s) 366, 787, 1074, 1321
Sau96I GGNCC 2 cut(s) 988, 1229
SchI GAGTC 4 cut(s) 644, 932, 934, 1019
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 3 cut(s) 359, 554, 1308
SfaNI GCATC 3 cut(s) 10, 326, 1282
SfcI CTRYAG 1 cut(s) 763
SfuI TTCGAA 1 cut(s) 1341
SinI GGWCC 2 cut(s) 988, 1229
SmiMI CAYNNNNRTG 3 cut(s) 312, 723, 1358
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SphI GCATGC 1 cut(s) 158
SsiI CCGC 3 cut(s) 76, 660, 1107
SspI AATATT 1 cut(s) 247
SspMI CTAG 3 cut(s) 432, 689, 1128
SstI GAGCTC 1 cut(s) 359
StyD4I CCNGG 1 cut(s) 655
TaaI ACNGT 3 cut(s) 242, 498, 817
TaiI ACGT 3 cut(s) 422, 845, 1303
TaqI TCGA 4 cut(s) 629, 921, 1173, 1341
TatI WGTACW 1 cut(s) 1119
TfiI GAWTC 6 cut(s) 50, 304, 435, 728, 856, 997
Tru1I TTAA 4 cut(s) 101, 174, 545, 1092
Tru9I TTAA 4 cut(s) 101, 174, 545, 1092
TscAI CASTG 3 cut(s) 376, 503, 820
TseFI GTSAC 2 cut(s) 133, 1011
TseI GCWGC 2 cut(s) 458, 765
Tsp45I GTSAC 2 cut(s) 133, 1011
TspDTI ATGAA 7 cut(s) 288, 296, 551, 897, 953, 1046, 1228
TspRI CASTG 3 cut(s) 376, 503, 820
Van91I CCANNNNNTGG 1 cut(s) 912
VpaK11BI GGWCC 2 cut(s) 988, 1229
XapI RAATTY 3 cut(s) 278, 1088, 1336
XceI RCATGY 2 cut(s) 158, 913
XmnI GAANNNNTTC 2 cut(s) 279, 303
XspI CTAG 3 cut(s) 432, 689, 1128
Zsp2I ATGCAT 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.