AT1G04960

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0010965
Physical Location & Seq
Reverse (-)
1407772 .. 1410933
3162 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G04960.1

Sequence Viewer

Length: 954 bp
ATGGCGATGCAAGCCGGTGTTCAGACCTCTAAGGTCCTTATCCTACTCGGCGCAGGTGTATCTGGTTCGATCGTATTGAGACATGGGAGGTTGTCTGACCTAATAGCCCAACTTCAAGACTTACTCAATGGTGCTCAAGGAGTTGAAAGCACTCCTTTTAAGTATGACGGTGCACTCCTTGCTGCTCAGATTCGACAGCTTGCAAACGAGATCAAAGAACTAACTATGACCAATCCAGTTACCATCTTTAATGGAGATTCAAACTCCAGTGGCTATGCATCTTACCTGGTTCCAGCGGCTGCCGTTGGAGCTATGGGGTACTGCTACATGTGGTGGAAGGGCTGGTCGTTTTCAGATGCAATGTTTGTGACAAAGAAAAATATGGCTGATGCGGTTGCCTCTGTTTCGAAACAACTAGACGATTTGTCAGACACATTAGCGTCAACAAGGAAACATCTTTCTCAGAAGCTAGCGACCTTGGATTGGAAGGTGGAAGAGCAGAATGAGACATCTAAAATGATCTTAAGTGATGTTACTGAGATGAGATCAAGCATTTCGCAAATTGGGTTTGATTTTAAGCAACTTAATGAAATGATTTCTGGAATAGAAGGGAAGATTGAAAGTCTTGAAAGCAAACAAGATGTGACCCTCTCTGGACTATGGCATCTATGTCAAGTTGCAGGCGTTAAAGACAGCACAAGTACTAAAGTATTTCAGGATGTTGGTGAGAGATTACCTATTGATGGAAAACCATCCACTAATACTTCTCTCTCTGGTCTGCGGTTTCTGACGGAAGGCAAAGAGGATGCAAATGTAATACACAAGCCGGTTATGGCGAAAGAAATTATGGTGATGGGGGAAAAAACAAAAGTCACTGCAGCGGGAAGAAGCAGAGTTCATAGAGCGTTTCCAGGTGGAATATCTTGGGTACGGGATGTTTCAGGACTATCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

317

Amino Acids

34.36

Weight (kDa)

6.54

Isoelectric Point (pI)

34.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 91 - 213 1.3e-48 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04960 AT1G04960
fragaria_vesca FvH4_6g41750 FvH4_6g41750
malus_domestica MD09G1117500.v1.1 MD17G1108700.v1.1
prunus_persica Prupe.3G206300_v2.0.a1 Prupe.3G206300_v2.0.a1
pyrus_communis pycom17g10140
rosa_chinensis RchiOBHm_Chr2g0157541
rosa_laevigata RLG00000020996
rosa_multiflora Rmu_sc0000195.1_g000040
rosa_roxburghii Rroxscaffold_2G00092140
rosa_rugosa Rorug02G0461900
rosa_samantha Rh2AG526000 Rh2BG540700 Rh2CG510700 Rh2DG549200 Rh4BG150000
rosa_wichuraiana Rw2G043570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AciI CCGC 4 cut(s) 296, 392, 781, 881
AfaI GTAC 3 cut(s) 320, 703, 930
AfiI CCNNNNNNNGG 2 cut(s) 483, 743
AflII CTTAAG 1 cut(s) 523
AflIII ACRYGT 1 cut(s) 327
AgsI TTSAA 5 cut(s) 116, 146, 261, 620, 629
AhdI GACNNNNNGTC 1 cut(s) 424
AjnI CCWGG 2 cut(s) 285, 910
AluBI AGCT 3 cut(s) 199, 311, 469
AluI AGCT 3 cut(s) 199, 311, 469
Alw21I GWGCWC 2 cut(s) 136, 175
Alw26I GTCTC 2 cut(s) 73, 500
Alw44I GTGCAC 1 cut(s) 171
AlwNI CAGNNNCTG 1 cut(s) 299
ApaLI GTGCAC 1 cut(s) 171
ApeKI GCWGC 3 cut(s) 182, 299, 878
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 1 cut(s) 34
AsuHPI GGTGA 2 cut(s) 737, 862
AsuII TTCGAA 1 cut(s) 407
AsuNHI GCTAGC 1 cut(s) 469
AvaII GGWCC 1 cut(s) 34
BaeGI GKGCMC 1 cut(s) 175
Bbv12I GWGCWC 2 cut(s) 136, 175
BbvI GCAGC 3 cut(s) 169, 286, 890
BccI CCATC 4 cut(s) 251, 737, 760, 847
BceAI ACGGC 1 cut(s) 287
BciT130I CCWGG 2 cut(s) 287, 912
BcoDI GTCTC 2 cut(s) 73, 500
BfaI CTAG 2 cut(s) 416, 470
BfmI CTRYAG 1 cut(s) 876
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 44
BisI GCNGC 4 cut(s) 183, 297, 300, 879
BlsI GCNGC 4 cut(s) 184, 298, 301, 880
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 2 cut(s) 287, 912
Bme18I GGWCC 1 cut(s) 34
BmeRI GACNNNNNGTC 1 cut(s) 424
BmgT120I GGNCC 1 cut(s) 34
BmiI GGNNCC 1 cut(s) 291
BmrFI CCNGG 2 cut(s) 287, 912
BmsI GCATC 5 cut(s) 287, 346, 379, 673, 796
BmtI GCTAGC 1 cut(s) 473
BpmI CTGGAG 1 cut(s) 250
Bpu14I TTCGAA 1 cut(s) 407
BpuEI CTTGAG 1 cut(s) 120
BsaJI CCNNGG 1 cut(s) 477
Bsc4I CCNNNNNNNGG 2 cut(s) 483, 743
Bse118I RCCGGY 2 cut(s) 14, 826
Bse1I ACTGG 2 cut(s) 236, 267
Bse3DI GCAATG 1 cut(s) 366
BseBI CCWGG 2 cut(s) 287, 912
BseDI CCNNGG 1 cut(s) 477
BseGI GGATG 4 cut(s) 724, 752, 811, 940
BseLI CCNNNNNNNGG 2 cut(s) 483, 743
BseMI GCAATG 1 cut(s) 366
BseMII CTCAG 3 cut(s) 200, 476, 528
BseNI ACTGG 2 cut(s) 236, 267
BseSI GKGCMC 1 cut(s) 175
BseXI GCAGC 3 cut(s) 169, 286, 890
Bsh1285I CGRYCG 1 cut(s) 72
BsiEI CGRYCG 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 136, 175
BsiSI CCGG 2 cut(s) 15, 827
BslI CCNNNNNNNGG 2 cut(s) 483, 743
BsmAI GTCTC 2 cut(s) 73, 500
Bsp119I TTCGAA 1 cut(s) 407
Bsp1286I GDGCHC 2 cut(s) 136, 175
Bsp143I GATC 4 cut(s) 69, 210, 519, 545
BspACI CCGC 4 cut(s) 296, 392, 781, 881
BspCNI CTCAG 3 cut(s) 199, 475, 529
BspLI GGNNCC 1 cut(s) 291
BspMAI CTGCAG 1 cut(s) 880
BspMI ACCTGC 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 473
BspQI GCTCTTC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 407
BspTI CTTAAG 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 366
BsrFI RCCGGY 2 cut(s) 14, 826
BsrI ACTGG 2 cut(s) 236, 267
BssAI RCCGGY 2 cut(s) 14, 826
BssECI CCNNGG 1 cut(s) 477
BssMI GATC 4 cut(s) 69, 210, 519, 545
BssT1I CCWWGG 1 cut(s) 477
Bst2UI CCWGG 2 cut(s) 287, 912
Bst4CI ACNGT 1 cut(s) 170
Bst6I CTCTTC 1 cut(s) 489
BstAFI CTTAAG 1 cut(s) 523
BstAPI GCANNNNNTGC 1 cut(s) 179
BstBI TTCGAA 1 cut(s) 407
BstC8I GCNNGC 4 cut(s) 12, 201, 471, 682
BstDEI CTNAG 4 cut(s) 30, 186, 462, 537
BstF5I GGATG 4 cut(s) 724, 752, 811, 940
BstHHI GCGC 1 cut(s) 53
BstKTI GATC 4 cut(s) 72, 213, 522, 548
BstMAI GTCTC 2 cut(s) 73, 500
BstMBI GATC 4 cut(s) 69, 210, 519, 545
BstMCI CGRYCG 1 cut(s) 72
BstMWI GCNNNNNNNGC 3 cut(s) 11, 179, 308
BstNI CCWGG 2 cut(s) 287, 912
BstNSI RCATGY 1 cut(s) 331
BstSCI CCNGG 2 cut(s) 285, 910
BstSFI CTRYAG 1 cut(s) 876
BstSLI GKGCMC 1 cut(s) 175
BstV1I GCAGC 3 cut(s) 169, 286, 890
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 4 cut(s) 724, 752, 811, 940
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 2 cut(s) 274, 873
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 4 cut(s) 12, 201, 471, 682
CaiI CAGNNNCTG 1 cut(s) 299
CfoI GCGC 1 cut(s) 53
Cfr10I RCCGGY 2 cut(s) 14, 826
Cfr13I GGNCC 1 cut(s) 34
CseI GACGC 1 cut(s) 429
CsiI ACCWGGT 1 cut(s) 285
Csp6I GTAC 3 cut(s) 319, 702, 929
CviAII CATG 2 cut(s) 83, 328
CviQI GTAC 3 cut(s) 319, 702, 929
DdeI CTNAG 4 cut(s) 30, 186, 462, 537
DpnI GATC 4 cut(s) 71, 212, 521, 547
DpnII GATC 4 cut(s) 69, 210, 519, 545
DriI GACNNNNNGTC 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 489
Eam1105I GACNNNNNGTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 489
Eco130I CCWWGG 1 cut(s) 477
Eco47I GGWCC 1 cut(s) 34
EcoO109I RGGNCCY 1 cut(s) 34
EcoRII CCWGG 2 cut(s) 285, 910
EcoT14I CCWWGG 1 cut(s) 477
EcoT22I ATGCAT 1 cut(s) 280
ErhI CCWWGG 1 cut(s) 477
FaeI CATG 2 cut(s) 86, 331
FalI AAGNNNNNCTT 2 cut(s) 139, 171
FatI CATG 2 cut(s) 82, 327
FauI CCCGC 1 cut(s) 874
Fnu4HI GCNGC 4 cut(s) 183, 297, 300, 879
FokI GGATG 4 cut(s) 731, 739, 818, 947
Fsp4HI GCNGC 4 cut(s) 183, 297, 300, 879
FspBI CTAG 2 cut(s) 416, 470
GlaI GCGC 1 cut(s) 52
GluI GCNGC 4 cut(s) 183, 297, 300, 879
GsuI CTGGAG 1 cut(s) 250
HapII CCGG 2 cut(s) 15, 827
HgaI GACGC 1 cut(s) 429
HhaI GCGC 1 cut(s) 53
Hin1II CATG 2 cut(s) 86, 331
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 2 cut(s) 190, 257
HpaII CCGG 2 cut(s) 15, 827
HphI GGTGA 2 cut(s) 737, 862
Hpy166II GTNNAC 2 cut(s) 173, 444
Hpy188I TCNGA 7 cut(s) 24, 97, 189, 355, 430, 465, 789
Hpy188III TCNNGA 6 cut(s) 116, 600, 626, 654, 716, 942
Hpy8I GTNNAC 2 cut(s) 173, 444
HpyAV CCTTC 4 cut(s) 331, 481, 602, 788
HpyCH4III ACNGT 1 cut(s) 170
HpyCH4V TGCA 8 cut(s) 10, 173, 203, 278, 359, 680, 809, 878
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 179, 308
HpyF3I CTNAG 4 cut(s) 30, 186, 462, 537
Hsp92II CATG 2 cut(s) 86, 331
HspAI GCGC 1 cut(s) 51
Kzo9I GATC 4 cut(s) 69, 210, 519, 545
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 308
Lsp1109I GCAGC 3 cut(s) 169, 286, 890
LweI GCATC 5 cut(s) 287, 346, 379, 673, 796
MabI ACCWGGT 1 cut(s) 285
MaeI CTAG 2 cut(s) 416, 470
MaeIII GTNAC 5 cut(s) 238, 367, 532, 643, 871
MalI GATC 4 cut(s) 71, 212, 521, 547
MboI GATC 4 cut(s) 69, 210, 519, 545
MboII GAAGA 3 cut(s) 506, 625, 897
MhlI GDGCHC 2 cut(s) 136, 175
MluCI AATT 2 cut(s) 561, 843
MmeI TCCRAC 1 cut(s) 286
MnlI CCTC 5 cut(s) 37, 81, 409, 659, 796
Mph1103I ATGCAT 1 cut(s) 280
MseI TTAA 6 cut(s) 159, 249, 524, 576, 585, 687
MspA1I CMGCKG 2 cut(s) 296, 881
MspCI CTTAAG 1 cut(s) 523
MspI CCGG 2 cut(s) 15, 827
MspR9I CCNGG 2 cut(s) 287, 912
MvaI CCWGG 2 cut(s) 287, 912
MwoI GCNNNNNNNGC 3 cut(s) 11, 179, 308
NdeII GATC 4 cut(s) 69, 210, 519, 545
NheI GCTAGC 1 cut(s) 469
NlaIII CATG 2 cut(s) 86, 331
NlaIV GGNNCC 1 cut(s) 291
NmeAIII GCCGAG 1 cut(s) 27
NmuCI GTSAC 3 cut(s) 367, 643, 871
NsiI ATGCAT 1 cut(s) 280
NspI RCATGY 1 cut(s) 331
NspV TTCGAA 1 cut(s) 407
PaqCI CACCTGC 1 cut(s) 44
PciI ACATGT 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 489
PfeI GAWTC 2 cut(s) 190, 257
PkrI GCNGC 4 cut(s) 184, 298, 301, 880
Ple19I CGATCG 1 cut(s) 72
PpuMI RGGWCCY 1 cut(s) 34
PscI ACATGT 1 cut(s) 327
Psp5II RGGWCCY 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 285, 910
PspGI CCWGG 2 cut(s) 285, 910
PspN4I GGNNCC 1 cut(s) 291
PspPI GGNCC 1 cut(s) 34
PspPPI RGGWCCY 1 cut(s) 34
PstI CTGCAG 1 cut(s) 880
PstNI CAGNNNCTG 1 cut(s) 299
PvuI CGATCG 1 cut(s) 72
RsaI GTAC 3 cut(s) 320, 703, 930
RsaNI GTAC 3 cut(s) 319, 702, 929
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 6 cut(s) 159, 249, 524, 576, 585, 687
SatI GCNGC 4 cut(s) 183, 297, 300, 879
Sau3AI GATC 4 cut(s) 69, 210, 519, 545
Sau96I GGNCC 1 cut(s) 34
ScaI AGTACT 1 cut(s) 703
ScrFI CCNGG 2 cut(s) 287, 912
SduI GDGCHC 2 cut(s) 136, 175
SexAI ACCWGGT 1 cut(s) 285
SfaNI GCATC 5 cut(s) 287, 346, 379, 673, 796
SfcI CTRYAG 1 cut(s) 876
SfuI TTCGAA 1 cut(s) 407
SinI GGWCC 1 cut(s) 34
SmlI CTYRAG 2 cut(s) 135, 523
SmoI CTYRAG 2 cut(s) 135, 523
Sse9I AATT 2 cut(s) 561, 843
SsiI CCGC 4 cut(s) 296, 392, 781, 881
SspMI CTAG 2 cut(s) 416, 470
StyD4I CCNGG 2 cut(s) 285, 910
StyI CCWWGG 1 cut(s) 477
TaaI ACNGT 1 cut(s) 170
TaqI TCGA 3 cut(s) 68, 193, 407
TasI AATT 2 cut(s) 561, 843
TatI WGTACW 1 cut(s) 701
TauI GCSGC 1 cut(s) 299
TfiI GAWTC 2 cut(s) 190, 257
Tru1I TTAA 6 cut(s) 159, 249, 524, 576, 585, 687
Tru9I TTAA 6 cut(s) 159, 249, 524, 576, 585, 687
TscAI CASTG 2 cut(s) 274, 880
TseFI GTSAC 3 cut(s) 367, 643, 871
TseI GCWGC 3 cut(s) 182, 299, 878
Tsp45I GTSAC 3 cut(s) 367, 643, 871
TspDTI ATGAA 2 cut(s) 603, 887
TspGWI ACGGA 1 cut(s) 806
TspRI CASTG 2 cut(s) 274, 880
Vha464I CTTAAG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 171
VpaK11BI GGWCC 1 cut(s) 34
XceI RCATGY 1 cut(s) 331
XspI CTAG 2 cut(s) 416, 470
ZrmI AGTACT 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 280
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.