MD17G1108700.v1.1

Protein of unknown function (DUF1664)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010965
Physical Location & Seq
Forward (+)
9322721 .. 9326151
3431 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1108700.v1.1.491

Sequence Viewer

Length: 960 bp
ATGGCGTTGCAGACGGGGGTTTCCGGCTCCAAGGTTCTCATCCTCGTCGGAGCAGGCTTGACCAGTTCGATAGTTTTGAGGAGCGGGCGATTGTCTGAACTTATTGCACAGCTTCAGGATATACTGAAGGGTGTAAATGAAGCTGATATCTTGCCTGAGAAGTTTGATAGTGCAATTATCAGAGCTCAGATTCAACAATTGGCTCGAGAAATCAGGGAGTTAACCTCATCTGGCCCTGTAACCGTCTACAATGAGAATTCTTCCTCTAGTGGGAGTTATGCTTCATATTTAGTTCCAGCTGCTGCAATTGGTGCAATGGGCTATTGTTACATGTGGTGGAAGGGCTGGTCATTTTCTGATGTAATGTTTGTAACAAGGCATAATATGGCAAATGCTGTTGCAACCGTGTCCAAACAATTAGAGAATGTGCATGAAACGTTGGCGTGTACAAAAAGGCATCTGTCGAAGAAGCTGGAAAACTTGGATTGGAAGGTGGAGGAGCAGAAGGAAATAACTCAGCTTATCGCAAATGATGTGAATGAGGTGAAAACAAACCTTTCTCAAATTGGGTTTGATGTAGAAATGATTCATCAGATGGTGTCTGGGTTGGAAGAGAAGGTCGACCTTATTGAGAGCAAACAGGATGCAACCAACTCAGGTCTATTGTATCTATGCCAAGTAGCAGATGGCGTCAAAGGTCAGCTGGATACTAAACCCTTACAGAATGTCTCAGCTAAGCTAGGAAAGCATTCACCAATGGCAATAGAGGATAAATCACCCAAGGGCCTTCAATTCTTTGCTGACAATGAAGAACAGAGCACAACGGAGAAATCAATGACAAAAAAGAATGATCCCAACATCTTCCCTGGAGAAAAAGTTCCAGCAATAAAAACAAGAATACACCGGTCATATCCAGTTGGGATTTCTTTGACCCGGGACAAAATGGGCTCGGGTATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1664

Protein Analysis

320

Amino Acids

35.03

Weight (kDa)

6.34

Isoelectric Point (pI)

48.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1664 PF07889 91 - 214 4e-50 Protein of unknown function (DUF1664)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010965)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G04960 AT1G04960
fragaria_vesca FvH4_6g41750 FvH4_6g41750
malus_domestica MD09G1117500.v1.1 MD17G1108700.v1.1
prunus_persica Prupe.3G206300_v2.0.a1 Prupe.3G206300_v2.0.a1
pyrus_communis pycom17g10140
rosa_chinensis RchiOBHm_Chr2g0157541
rosa_laevigata RLG00000020996
rosa_multiflora Rmu_sc0000195.1_g000040
rosa_roxburghii Rroxscaffold_2G00092140
rosa_rugosa Rorug02G0461900
rosa_samantha Rh2AG526000 Rh2BG540700 Rh2CG510700 Rh2DG549200 Rh4BG150000
rosa_wichuraiana Rw2G043570

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 84
AccI GTMKAC 2 cut(s) 246, 621
AciI CCGC 1 cut(s) 84
AclI AACGTT 1 cut(s) 437
AclWI GGATC 1 cut(s) 845
AcsI RAATTY 1 cut(s) 256
AcuI CTGAAG 2 cut(s) 98, 146
AcyI GRCGYC 1 cut(s) 690
AfaI GTAC 1 cut(s) 448
AfiI CCNNNNNNNGG 1 cut(s) 270
AflIII ACRYGT 1 cut(s) 330
AgeI ACCGGT 1 cut(s) 903
AgsI TTSAA 2 cut(s) 194, 791
AjnI CCWGG 1 cut(s) 865
AloI GAACNNNNNNTCC 2 cut(s) 861, 893
AluBI AGCT 9 cut(s) 112, 143, 185, 299, 472, 520, 703, 734, 739
AluI AGCT 9 cut(s) 112, 143, 185, 299, 472, 520, 703, 734, 739
Alw21I GWGCWC 2 cut(s) 187, 821
Alw26I GTCTC 1 cut(s) 733
AlwI GGATC 1 cut(s) 845
AlwNI CAGNNNCTG 1 cut(s) 302
Ama87I CYCGRG 3 cut(s) 204, 933, 949
AoxI GGCC 2 cut(s) 232, 784
ApeKI GCWGC 2 cut(s) 299, 302
ApoI RAATTY 1 cut(s) 256
AsiGI ACCGGT 1 cut(s) 903
Asp700I GAANNNNTTC 3 cut(s) 585, 748, 876
AspS9I GGNCC 2 cut(s) 233, 784
AsuC2I CCSGG 2 cut(s) 934, 935
AsuHPI GGTGA 3 cut(s) 556, 744, 768
AvaI CYCGRG 3 cut(s) 204, 933, 949
BanII GRGCYC 2 cut(s) 187, 950
Bbv12I GWGCWC 2 cut(s) 187, 821
BbvI GCAGC 2 cut(s) 286, 289
BccI CCATC 2 cut(s) 589, 680
BciT130I CCWGG 1 cut(s) 867
BciVI GTATCC 1 cut(s) 700
BcnI CCSGG 2 cut(s) 934, 935
BcoDI GTCTC 1 cut(s) 733
BfaI CTAG 2 cut(s) 267, 740
BfuI GTATCC 1 cut(s) 700
BisI GCNGC 2 cut(s) 300, 303
BlpI GCTNAGC 1 cut(s) 735
BlsI GCNGC 2 cut(s) 301, 304
Bme1390I CCNGG 3 cut(s) 867, 934, 935
BmeT110I CYCGRG 3 cut(s) 204, 933, 949
BmgT120I GGNCC 2 cut(s) 233, 784
BmiI GGNNCC 1 cut(s) 28
BmrFI CCNGG 3 cut(s) 867, 934, 935
BmsI GCATC 2 cut(s) 466, 634
BplI GAGNNNNNCTC 2 cut(s) 209, 241
BpmI CTGGAG 1 cut(s) 888
Bpu1102I GCTNAGC 1 cut(s) 735
BpuMI CCSGG 2 cut(s) 934, 935
BsaHI GRCGYC 1 cut(s) 690
BsaJI CCNNGG 4 cut(s) 30, 780, 865, 933
BsaWI WCCGGW 1 cut(s) 903
BsaXI ACNNNNNCTCC 2 cut(s) 861, 891
Bsc4I CCNNNNNNNGG 1 cut(s) 270
Bse118I RCCGGY 1 cut(s) 903
Bse1I ACTGG 2 cut(s) 63, 914
Bse3DI GCAATG 1 cut(s) 321
BseBI CCWGG 1 cut(s) 867
BseDI CCNNGG 4 cut(s) 30, 780, 865, 933
BseGI GGATG 2 cut(s) 39, 649
BseLI CCNNNNNNNGG 1 cut(s) 270
BseMI GCAATG 1 cut(s) 321
BseMII CTCAG 5 cut(s) 147, 200, 530, 669, 744
BseNI ACTGG 2 cut(s) 63, 914
BseRI GAGGAG 2 cut(s) 94, 512
BseXI GCAGC 2 cut(s) 286, 289
BshFI GGCC 2 cut(s) 234, 786
BshTI ACCGGT 1 cut(s) 903
BsiHKAI GWGCWC 2 cut(s) 187, 821
BsiHKCI CYCGRG 3 cut(s) 204, 933, 949
BsiSI CCGG 3 cut(s) 24, 904, 934
BslFI GGGAC 1 cut(s) 950
BslI CCNNNNNNNGG 1 cut(s) 270
BsmAI GTCTC 1 cut(s) 733
BsmFI GGGAC 1 cut(s) 950
BsmI GAATGC 1 cut(s) 748
BsnI GGCC 2 cut(s) 234, 786
BsoBI CYCGRG 3 cut(s) 204, 933, 949
Bsp1286I GDGCHC 3 cut(s) 187, 821, 950
Bsp1407I TGTACA 1 cut(s) 446
Bsp143I GATC 1 cut(s) 850
Bsp1720I GCTNAGC 1 cut(s) 735
BspACI CCGC 1 cut(s) 84
BspANI GGCC 2 cut(s) 234, 786
BspCNI CTCAG 5 cut(s) 148, 199, 529, 668, 743
BspLI GGNNCC 1 cut(s) 28
BspPI GGATC 1 cut(s) 845
BsrBI CCGCTC 1 cut(s) 84
BsrDI GCAATG 1 cut(s) 321
BsrFI RCCGGY 1 cut(s) 903
BsrGI TGTACA 1 cut(s) 446
BsrI ACTGG 2 cut(s) 63, 914
BssAI RCCGGY 1 cut(s) 903
BssECI CCNNGG 4 cut(s) 30, 780, 865, 933
BssMI GATC 1 cut(s) 850
BssNI GRCGYC 1 cut(s) 690
BssT1I CCWWGG 2 cut(s) 30, 780
Bst2UI CCWGG 1 cut(s) 867
Bst4CI ACNGT 2 cut(s) 244, 406
Bst6I CTCTTC 1 cut(s) 606
BstACI GRCGYC 1 cut(s) 690
BstAPI GCANNNNNTGC 1 cut(s) 311
BstAUI TGTACA 1 cut(s) 446
BstC8I GCNNGC 2 cut(s) 55, 86
BstDEI CTNAG 6 cut(s) 156, 186, 516, 655, 730, 735
BstF5I GGATG 2 cut(s) 39, 649
BstKTI GATC 1 cut(s) 853
BstMAI GTCTC 1 cut(s) 733
BstMBI GATC 1 cut(s) 850
BstMWI GCNNNNNNNGC 2 cut(s) 311, 745
BstNI CCWGG 1 cut(s) 867
BstNSI RCATGY 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 865, 932, 933
BstV1I GCAGC 2 cut(s) 286, 289
BsuI GTATCC 1 cut(s) 700
BsuRI GGCC 2 cut(s) 234, 786
BtsCI GGATG 2 cut(s) 39, 649
Cac8I GCNNGC 2 cut(s) 55, 86
CaiI CAGNNNCTG 1 cut(s) 302
Cfr10I RCCGGY 1 cut(s) 903
Cfr13I GGNCC 2 cut(s) 233, 784
Cfr9I CCCGGG 1 cut(s) 933
CseI GACGC 1 cut(s) 679
Csp6I GTAC 1 cut(s) 447
CspAI ACCGGT 1 cut(s) 903
CviAII CATG 2 cut(s) 331, 431
CviQI GTAC 1 cut(s) 447
DdeI CTNAG 6 cut(s) 156, 186, 516, 655, 730, 735
DpnI GATC 1 cut(s) 852
DpnII GATC 1 cut(s) 850
Eam1104I CTCTTC 1 cut(s) 606
EarI CTCTTC 1 cut(s) 606
Ecl136II GAGCTC 1 cut(s) 185
Eco130I CCWWGG 2 cut(s) 30, 780
Eco24I GRGCYC 2 cut(s) 187, 950
Eco32I GATATC 1 cut(s) 148
Eco53kI GAGCTC 1 cut(s) 185
Eco57I CTGAAG 2 cut(s) 98, 146
Eco88I CYCGRG 3 cut(s) 204, 933, 949
EcoICRI GAGCTC 1 cut(s) 185
EcoO109I RGGNCCY 1 cut(s) 784
EcoRI GAATTC 1 cut(s) 256
EcoRII CCWGG 1 cut(s) 865
EcoRV GATATC 1 cut(s) 148
EcoT14I CCWWGG 2 cut(s) 30, 780
EcoT38I GRGCYC 2 cut(s) 187, 950
ErhI CCWWGG 2 cut(s) 30, 780
FaeI CATG 2 cut(s) 334, 434
FaqI GGGAC 1 cut(s) 950
FatI CATG 2 cut(s) 330, 430
FauI CCCGC 1 cut(s) 77
FblI GTMKAC 2 cut(s) 246, 621
Fnu4HI GCNGC 2 cut(s) 300, 303
FokI GGATG 2 cut(s) 26, 656
FriOI GRGCYC 2 cut(s) 187, 950
Fsp4HI GCNGC 2 cut(s) 300, 303
FspBI CTAG 2 cut(s) 267, 740
GluI GCNGC 2 cut(s) 300, 303
GsuI CTGGAG 1 cut(s) 888
HaeIII GGCC 2 cut(s) 234, 786
HapII CCGG 3 cut(s) 24, 904, 934
HgaI GACGC 1 cut(s) 679
Hin1I GRCGYC 1 cut(s) 690
Hin1II CATG 2 cut(s) 334, 434
HincII GTYRAC 2 cut(s) 222, 622
HindII GTYRAC 2 cut(s) 222, 622
HinfI GANTC 2 cut(s) 190, 586
HpaI GTTAAC 1 cut(s) 222
HpaII CCGG 3 cut(s) 24, 904, 934
HphI GGTGA 3 cut(s) 556, 744, 768
Hpy166II GTNNAC 4 cut(s) 222, 247, 447, 622
Hpy188I TCNGA 6 cut(s) 50, 97, 182, 189, 358, 594
Hpy188III TCNNGA 2 cut(s) 116, 206
Hpy8I GTNNAC 4 cut(s) 222, 247, 447, 622
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 6 cut(s) 121, 334, 484, 499, 610, 797
HpyCH4III ACNGT 2 cut(s) 244, 406
HpyCH4IV ACGT 1 cut(s) 437
HpyCH4V TGCA 8 cut(s) 10, 107, 173, 305, 314, 401, 430, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 311, 745
HpyF3I CTNAG 6 cut(s) 156, 186, 516, 655, 730, 735
HpySE526I ACGT 1 cut(s) 437
Hsp92I GRCGYC 1 cut(s) 690
Hsp92II CATG 2 cut(s) 334, 434
KspAI GTTAAC 1 cut(s) 222
Kzo9I GATC 1 cut(s) 850
LmnI GCTCC 4 cut(s) 32, 50, 81, 499
Lsp1109I GCAGC 2 cut(s) 286, 289
LweI GCATC 2 cut(s) 466, 634
MaeI CTAG 2 cut(s) 267, 740
MaeII ACGT 1 cut(s) 437
MaeIII GTNAC 3 cut(s) 238, 326, 370
MalI GATC 1 cut(s) 852
MbiI CCGCTC 1 cut(s) 84
MboI GATC 1 cut(s) 850
MboII GAAGA 5 cut(s) 252, 478, 623, 821, 853
MfeI CAATTG 2 cut(s) 197, 306
MhlI GDGCHC 3 cut(s) 187, 821, 950
MluCI AATT 7 cut(s) 174, 197, 256, 306, 416, 564, 791
MmeI TCCRAC 2 cut(s) 28, 588
MnlI CCTC 7 cut(s) 53, 72, 235, 274, 490, 535, 760
MroXI GAANNNNTTC 3 cut(s) 585, 748, 876
MseI TTAA 1 cut(s) 221
MspA1I CMGCKG 2 cut(s) 299, 703
MspI CCGG 3 cut(s) 24, 904, 934
MspR9I CCNGG 3 cut(s) 867, 934, 935
MunI CAATTG 2 cut(s) 197, 306
Mva1269I GAATGC 1 cut(s) 748
MvaI CCWGG 1 cut(s) 867
MwoI GCNNNNNNNGC 2 cut(s) 311, 745
NciI CCSGG 2 cut(s) 934, 935
NdeII GATC 1 cut(s) 850
NlaIII CATG 2 cut(s) 334, 434
NlaIV GGNNCC 1 cut(s) 28
NspI RCATGY 1 cut(s) 334
PaeR7I CTCGAG 1 cut(s) 204
PciI ACATGT 1 cut(s) 330
PctI GAATGC 1 cut(s) 748
PdmI GAANNNNTTC 3 cut(s) 585, 748, 876
PfeI GAWTC 2 cut(s) 190, 586
PinAI ACCGGT 1 cut(s) 903
PkrI GCNGC 2 cut(s) 301, 304
PscI ACATGT 1 cut(s) 330
Psp124BI GAGCTC 1 cut(s) 187
Psp1406I AACGTT 1 cut(s) 437
Psp6I CCWGG 1 cut(s) 865
PspGI CCWGG 1 cut(s) 865
PspN4I GGNNCC 1 cut(s) 28
PspPI GGNCC 2 cut(s) 233, 784
PstNI CAGNNNCTG 1 cut(s) 302
PvuII CAGCTG 2 cut(s) 299, 703
RsaI GTAC 1 cut(s) 448
RsaNI GTAC 1 cut(s) 447
SacI GAGCTC 1 cut(s) 187
SalI GTCGAC 1 cut(s) 620
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 2 cut(s) 300, 303
Sau3AI GATC 1 cut(s) 850
Sau96I GGNCC 2 cut(s) 233, 784
ScrFI CCNGG 3 cut(s) 867, 934, 935
SduI GDGCHC 3 cut(s) 187, 821, 950
SfaNI GCATC 2 cut(s) 466, 634
Sfr274I CTCGAG 1 cut(s) 204
SlaI CTCGAG 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 935
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 7 cut(s) 174, 197, 256, 306, 416, 564, 791
SsiI CCGC 1 cut(s) 84
SspMI CTAG 2 cut(s) 267, 740
SstI GAGCTC 1 cut(s) 187
StyD4I CCNGG 3 cut(s) 865, 932, 933
StyI CCWWGG 2 cut(s) 30, 780
TaaI ACNGT 2 cut(s) 244, 406
TaiI ACGT 1 cut(s) 440
TaqI TCGA 4 cut(s) 68, 205, 464, 621
TasI AATT 7 cut(s) 174, 197, 256, 306, 416, 564, 791
TatI WGTACW 1 cut(s) 446
TfiI GAWTC 2 cut(s) 190, 586
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TseI GCWGC 2 cut(s) 299, 302
TspDTI ATGAA 5 cut(s) 153, 273, 447, 578, 822
TspGWI ACGGA 1 cut(s) 839
TspMI CCCGGG 1 cut(s) 933
XapI RAATTY 1 cut(s) 256
XceI RCATGY 1 cut(s) 334
XcmI CCANNNNNNNNNTGG 1 cut(s) 683
XhoI CTCGAG 1 cut(s) 204
XmaI CCCGGG 1 cut(s) 933
XmiI GTMKAC 2 cut(s) 246, 621
XmnI GAANNNNTTC 3 cut(s) 585, 748, 876
XspI CTAG 2 cut(s) 267, 740
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.