AT1G05300

zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009811
Physical Location & Seq
Reverse (-)
1544927 .. 1547849
2923 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G05300.1

Sequence Viewer

Length: 1083 bp
ATGAGAATCACACAAAACGTCAAGCTCTTACTCTTCTTCTTCTTCTTCATCTCCTTCCTCTTCATCGCCGTATCCGCCGGCGAGTCTAAATGCGAGTGTTCACACGAAGACGACGAGGCAAACAAAGCCGGAGCAAAAAAATACAAGATCGCCGCAATTCCTTCCGTTCTGGCCGCCGGAGTTATCGGCGTTATGTTTCCGTTACTAGGCAAGTTCTTCCCGTCGCTTAAACCGGAGACGACTTTCTTCTTCGTGACGAAAGCCTTCGCCGCCGGCGTAATCCTCGCAACGGGGTTTATGCATGTGTTGCCTGAAGGTTACGAGAAGCTGACGTCACCGTGTCTGAAAGGAGAAGCCTGGGAGTTTCCGTTCACAGGCTTTATCGCGATGGTCGCTGCGATTTTGACTCTTTCCGTTGACTCTTTTGCCACGTCGTATTTCCATAAAGCGCATTTTAAAACGTCTAAGAGGATTGGTGACGGTGAGGAACAAGACGCTGGCGGTGGCGGTGGCGGTGGAGACGAGCTCGGGTTGCACGTGCATGCTCACGGTCACACGCACGGGATTGTTGGCGTGGAATCTGGTGAATCCCAGGTTCAGCTTCACCGGACTCGAGTTGTTGCACAGGTGTTGGAAGTGGGAATAATAGTACATTCAGTGGTAATAGGAATATCACTGGGAGCATCACAAAGTCCAGACACTGCCAAAGCTCTCTTTGCTGCTTTAATGTTTCATCAATGCTTCGAGGGTCTTGGTCTTGGTGGTTGCATCGCCCAGGGAAATTTCAATTGTATGTCAATTACAATAATGTCGATCTTTTTCTCTGTAACGACGCCGGTAGGAATCGCGGTTGGAATGGCAATTTCAAGCTCTTATGATGATTCGAGTCCAACGGCTCTGATCGTACAGGGAGTCCTTAACGCTGCATCTGCAGGCATCCTCATCTATATGTCTCTAGTGGACTTTCTCGCAGCAGATTTTATGCACCCTAAGATGCAGTCTAACACTAGACTTCAAATCATGGCTCATATCTCTCTTCTTGTTGGAGCAGGGGTTATGTCACTTTTAGCTAAATGGGCTTAA

Protein Analysis

360

Amino Acids

38.17

Weight (kDa)

6.26

Isoelectric Point (pI)

36.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 48 - 357 6.5e-80 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 335
AccII CGCG 2 cut(s) 386, 848
AciI CCGC 8 cut(s) 75, 153, 174, 270, 501, 507, 513, 848
AcoI YGGCCR 1 cut(s) 171
AcsI RAATTY 1 cut(s) 781
AcuI CTGAAG 1 cut(s) 333
AcvI CACGTG 1 cut(s) 538
AcyI GRCGYC 2 cut(s) 332, 833
AfaI GTAC 2 cut(s) 651, 906
AfiI CCNNNNNNNGG 3 cut(s) 206, 289, 374
AgsI TTSAA 3 cut(s) 787, 867, 1016
AjiI CACGTC 1 cut(s) 432
AjnI CCWGG 3 cut(s) 356, 591, 774
AloI GAACNNNNNNTCC 2 cut(s) 353, 385
AluBI AGCT 7 cut(s) 25, 328, 526, 601, 710, 870, 1070
AluI AGCT 7 cut(s) 25, 328, 526, 601, 710, 870, 1070
Alw21I GWGCWC 1 cut(s) 528
Alw26I GTCTC 3 cut(s) 230, 513, 957
AlwNI CAGNNNCTG 1 cut(s) 701
Ama87I CYCGRG 2 cut(s) 527, 612
AoxI GGCC 1 cut(s) 171
ApeKI GCWGC 4 cut(s) 395, 719, 923, 971
ApoI RAATTY 1 cut(s) 781
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 451
AsuHPI GGTGA 5 cut(s) 327, 488, 494, 596, 596
AvaI CYCGRG 2 cut(s) 527, 612
BanII GRGCYC 1 cut(s) 528
BbrPI CACGTG 1 cut(s) 538
BbsI GAAGAC 1 cut(s) 114
Bbv12I GWGCWC 1 cut(s) 528
BbvI GCAGC 4 cut(s) 382, 706, 910, 983
BccI CCATC 1 cut(s) 382
BceAI ACGGC 2 cut(s) 53, 909
BciT130I CCWGG 3 cut(s) 358, 593, 776
BciVI GTATCC 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 230, 513, 957
BfaI CTAG 3 cut(s) 206, 956, 1008
BfmI CTRYAG 1 cut(s) 930
BfuI GTATCC 1 cut(s) 82
BisI GCNGC 7 cut(s) 153, 174, 270, 396, 720, 924, 972
BlsI GCNGC 7 cut(s) 154, 175, 271, 397, 721, 925, 973
Bme1390I CCNGG 3 cut(s) 358, 593, 776
BmeT110I CYCGRG 2 cut(s) 527, 612
BmgBI CACGTC 1 cut(s) 432
BmrFI CCNGG 3 cut(s) 358, 593, 776
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 5 cut(s) 692, 777, 935, 945, 984
BmuI ACTGGG 1 cut(s) 686
BpiI GAAGAC 1 cut(s) 114
BplI GAGNNNNNCTC 2 cut(s) 510, 542
BsaAI YACGTR 1 cut(s) 538
BsaHI GRCGYC 2 cut(s) 332, 833
BsaJI CCNNGG 4 cut(s) 357, 591, 774, 775
BsaWI WCCGGW 2 cut(s) 232, 606
BsaXI ACNNNNNCTCC 4 cut(s) 353, 383, 1038, 1068
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 289, 374
Bse118I RCCGGY 3 cut(s) 77, 272, 835
Bse1I ACTGG 1 cut(s) 681
BseBI CCWGG 3 cut(s) 358, 593, 776
BseDI CCNNGG 4 cut(s) 357, 591, 774, 775
BseGI GGATG 1 cut(s) 936
BseLI CCNNNNNNNGG 3 cut(s) 206, 289, 374
BseNI ACTGG 1 cut(s) 681
BseXI GCAGC 4 cut(s) 382, 706, 910, 983
Bsh1236I CGCG 2 cut(s) 386, 848
BshFI GGCC 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 528
BsiHKCI CYCGRG 2 cut(s) 527, 612
BsiSI CCGG 7 cut(s) 78, 129, 177, 233, 273, 607, 836
BslI CCNNNNNNNGG 3 cut(s) 206, 289, 374
BsmAI GTCTC 3 cut(s) 230, 513, 957
BsmBI CGTCTC 2 cut(s) 230, 513
BsnI GGCC 1 cut(s) 173
BsoBI CYCGRG 2 cut(s) 527, 612
Bsp1286I GDGCHC 1 cut(s) 528
Bsp143I GATC 3 cut(s) 147, 813, 900
Bsp68I TCGCGA 1 cut(s) 386
BspACI CCGC 8 cut(s) 75, 153, 174, 270, 501, 507, 513, 848
BspANI GGCC 1 cut(s) 173
BspFNI CGCG 2 cut(s) 386, 848
BspMAI CTGCAG 1 cut(s) 934
BsrFI RCCGGY 3 cut(s) 77, 272, 835
BsrI ACTGG 1 cut(s) 681
BssAI RCCGGY 3 cut(s) 77, 272, 835
BssECI CCNNGG 4 cut(s) 357, 591, 774, 775
BssMI GATC 3 cut(s) 147, 813, 900
BssNI GRCGYC 2 cut(s) 332, 833
Bst2UI CCWGG 3 cut(s) 358, 593, 776
Bst4CI ACNGT 3 cut(s) 339, 482, 551
Bst6I CTCTTC 3 cut(s) 38, 65, 1041
BstACI GRCGYC 2 cut(s) 332, 833
BstAPI GCANNNNNTGC 1 cut(s) 307
BstBAI YACGTR 1 cut(s) 538
BstC8I GCNNGC 5 cut(s) 79, 274, 499, 543, 934
BstDEI CTNAG 2 cut(s) 465, 990
BstF5I GGATG 1 cut(s) 936
BstFNI CGCG 2 cut(s) 386, 848
BstHHI GCGC 1 cut(s) 451
BstKTI GATC 3 cut(s) 150, 816, 903
BstMAI GTCTC 3 cut(s) 230, 513, 957
BstMBI GATC 3 cut(s) 147, 813, 900
BstMWI GCNNNNNNNGC 9 cut(s) 74, 125, 269, 307, 392, 532, 716, 929, 1076
BstNI CCWGG 3 cut(s) 358, 593, 776
BstNSI RCATGY 2 cut(s) 305, 545
BstSCI CCNGG 3 cut(s) 356, 591, 774
BstSFI CTRYAG 1 cut(s) 930
BstUI CGCG 2 cut(s) 386, 848
BstV1I GCAGC 4 cut(s) 382, 706, 910, 983
BstV2I GAAGAC 1 cut(s) 114
BsuI GTATCC 1 cut(s) 82
BsuRI GGCC 1 cut(s) 173
BtgZI GCGATG 3 cut(s) 49, 401, 754
BtrI CACGTC 1 cut(s) 432
BtsCI GGATG 1 cut(s) 936
BtsI GCAGTG 1 cut(s) 699
BtsIMutI CAGTG 3 cut(s) 663, 674, 699
BtuMI TCGCGA 1 cut(s) 386
Cac8I GCNNGC 5 cut(s) 79, 274, 499, 543, 934
CaiI CAGNNNCTG 1 cut(s) 701
CfoI GCGC 1 cut(s) 451
Cfr10I RCCGGY 3 cut(s) 77, 272, 835
CseI GACGC 2 cut(s) 503, 841
Csp6I GTAC 2 cut(s) 650, 905
CviAII CATG 3 cut(s) 302, 542, 1021
CviQI GTAC 2 cut(s) 650, 905
DdeI CTNAG 2 cut(s) 465, 990
DpnI GATC 3 cut(s) 149, 815, 902
DpnII GATC 3 cut(s) 147, 813, 900
DraI TTTAAA 1 cut(s) 457
EaeI YGGCCR 1 cut(s) 171
Eam1104I CTCTTC 3 cut(s) 38, 65, 1041
EarI CTCTTC 3 cut(s) 38, 65, 1041
EciI GGCGGA 1 cut(s) 64
Ecl136II GAGCTC 1 cut(s) 526
Eco24I GRGCYC 1 cut(s) 528
Eco53kI GAGCTC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 333
Eco72I CACGTG 1 cut(s) 538
Eco88I CYCGRG 2 cut(s) 527, 612
EcoICRI GAGCTC 1 cut(s) 526
EcoRII CCWGG 3 cut(s) 356, 591, 774
EcoT22I ATGCAT 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 528
Esp3I CGTCTC 2 cut(s) 230, 513
FaeI CATG 3 cut(s) 305, 545, 1024
FatI CATG 3 cut(s) 301, 541, 1020
Fnu4HI GCNGC 7 cut(s) 153, 174, 270, 396, 720, 924, 972
FokI GGATG 1 cut(s) 923
FriOI GRGCYC 1 cut(s) 528
Fsp4HI GCNGC 7 cut(s) 153, 174, 270, 396, 720, 924, 972
FspBI CTAG 3 cut(s) 206, 956, 1008
GlaI GCGC 1 cut(s) 450
GluI GCNGC 7 cut(s) 153, 174, 270, 396, 720, 924, 972
HaeIII GGCC 1 cut(s) 173
HapII CCGG 7 cut(s) 78, 129, 177, 233, 273, 607, 836
HgaI GACGC 2 cut(s) 503, 841
HhaI GCGC 1 cut(s) 451
Hin1I GRCGYC 2 cut(s) 332, 833
Hin1II CATG 3 cut(s) 305, 545, 1024
Hin6I GCGC 1 cut(s) 449
HinP1I GCGC 1 cut(s) 449
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HpaII CCGG 7 cut(s) 78, 129, 177, 233, 273, 607, 836
HphI GGTGA 5 cut(s) 327, 488, 494, 596, 596
Hpy166II GTNNAC 4 cut(s) 101, 372, 418, 961
Hpy188I TCNGA 2 cut(s) 345, 900
Hpy188III TCNNGA 3 cut(s) 253, 385, 695
Hpy8I GTNNAC 4 cut(s) 101, 372, 418, 961
Hpy99I CGWCG 4 cut(s) 116, 226, 436, 835
HpyAV CCTTC 4 cut(s) 64, 171, 274, 308
HpyCH4III ACNGT 3 cut(s) 339, 482, 551
HpyCH4IV ACGT 5 cut(s) 18, 332, 431, 461, 537
HpyCH4V TGCA 9 cut(s) 301, 535, 541, 623, 768, 926, 932, 985, 997
HpyF10VI GCNNNNNNNGC 9 cut(s) 74, 125, 269, 307, 392, 532, 716, 929, 1076
HpyF3I CTNAG 2 cut(s) 465, 990
HpySE526I ACGT 5 cut(s) 18, 332, 431, 461, 537
Hsp92I GRCGYC 2 cut(s) 332, 833
Hsp92II CATG 3 cut(s) 305, 545, 1024
HspAI GCGC 1 cut(s) 449
KroI GCCGGC 2 cut(s) 77, 272
KroNI GCCGGC 2 cut(s) 79, 274
Kzo9I GATC 3 cut(s) 147, 813, 900
LmnI GCTCC 3 cut(s) 131, 680, 1046
Lsp1109I GCAGC 4 cut(s) 382, 706, 910, 983
LweI GCATC 5 cut(s) 692, 777, 935, 945, 984
MaeI CTAG 3 cut(s) 206, 956, 1008
MaeII ACGT 5 cut(s) 18, 332, 431, 461, 537
MaeIII GTNAC 8 cut(s) 201, 253, 317, 333, 476, 551, 826, 1059
MalI GATC 3 cut(s) 149, 815, 902
MboI GATC 3 cut(s) 147, 813, 900
MfeI CAATTG 1 cut(s) 787
MhlI GDGCHC 1 cut(s) 528
MluCI AATT 5 cut(s) 156, 781, 787, 798, 861
MlyI GAGTC 6 cut(s) 92, 400, 413, 604, 895, 921
MmeI TCCRAC 4 cut(s) 612, 832, 914, 1024
MnlI CCTC 7 cut(s) 68, 109, 293, 462, 478, 739, 950
Mph1103I ATGCAT 1 cut(s) 303
MreI CGCCGGCG 2 cut(s) 77, 272
MroNI GCCGGC 2 cut(s) 77, 272
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 5 cut(s) 228, 456, 725, 918, 1081
MslI CAYNNNNRTG 2 cut(s) 540, 947
MspI CCGG 7 cut(s) 78, 129, 177, 233, 273, 607, 836
MspR9I CCNGG 3 cut(s) 358, 593, 776
MunI CAATTG 1 cut(s) 787
MvaI CCWGG 3 cut(s) 358, 593, 776
MvnI CGCG 2 cut(s) 386, 848
MwoI GCNNNNNNNGC 9 cut(s) 74, 125, 269, 307, 392, 532, 716, 929, 1076
NaeI GCCGGC 2 cut(s) 79, 274
NdeII GATC 3 cut(s) 147, 813, 900
NgoMIV GCCGGC 2 cut(s) 77, 272
NlaIII CATG 3 cut(s) 305, 545, 1024
NmuCI GTSAC 5 cut(s) 253, 333, 476, 551, 1059
NruI TCGCGA 1 cut(s) 386
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 2 cut(s) 305, 545
PaeI GCATGC 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 612
PasI CCCWGGG 1 cut(s) 775
PcsI WCGNNNNNNNCGW 3 cut(s) 111, 273, 534
PdiI GCCGGC 2 cut(s) 79, 274
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 5 cut(s) 6, 578, 587, 843, 881
PkrI GCNGC 7 cut(s) 154, 175, 271, 397, 721, 925, 973
PleI GAGTC 6 cut(s) 91, 400, 413, 604, 894, 920
PmaCI CACGTG 1 cut(s) 538
PmlI CACGTG 1 cut(s) 538
PpsI GAGTC 6 cut(s) 91, 400, 413, 604, 894, 920
Ppu21I YACGTR 1 cut(s) 538
Psp124BI GAGCTC 1 cut(s) 528
Psp6I CCWGG 3 cut(s) 356, 591, 774
PspCI CACGTG 1 cut(s) 538
PspGI CCWGG 3 cut(s) 356, 591, 774
PspXI VCTCGAGB 1 cut(s) 612
PstI CTGCAG 1 cut(s) 934
PstNI CAGNNNCTG 1 cut(s) 701
RruI TCGCGA 1 cut(s) 386
RsaI GTAC 2 cut(s) 651, 906
RsaNI GTAC 2 cut(s) 650, 905
RseI CAYNNNNRTG 2 cut(s) 540, 947
SacI GAGCTC 1 cut(s) 528
SaqAI TTAA 5 cut(s) 228, 456, 725, 918, 1081
SatI GCNGC 7 cut(s) 153, 174, 270, 396, 720, 924, 972
Sau3AI GATC 3 cut(s) 147, 813, 900
SchI GAGTC 6 cut(s) 92, 400, 413, 604, 895, 921
ScrFI CCNGG 3 cut(s) 358, 593, 776
SduI GDGCHC 1 cut(s) 528
SfaNI GCATC 5 cut(s) 692, 777, 935, 945, 984
SfcI CTRYAG 1 cut(s) 930
Sfr274I CTCGAG 1 cut(s) 612
SgrAI CRCCGGYG 2 cut(s) 77, 272
SlaI CTCGAG 1 cut(s) 612
SmiMI CAYNNNNRTG 2 cut(s) 540, 947
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SphI GCATGC 1 cut(s) 545
Sse9I AATT 5 cut(s) 156, 781, 787, 798, 861
SsiI CCGC 8 cut(s) 75, 153, 174, 270, 501, 507, 513, 848
SspMI CTAG 3 cut(s) 206, 956, 1008
SstI GAGCTC 1 cut(s) 528
StyD4I CCNGG 3 cut(s) 356, 591, 774
TaaI ACNGT 3 cut(s) 339, 482, 551
TaiI ACGT 5 cut(s) 21, 335, 434, 464, 540
TaqI TCGA 4 cut(s) 613, 744, 812, 884
TasI AATT 5 cut(s) 156, 781, 787, 798, 861
TatI WGTACW 1 cut(s) 649
TauI GCSGC 3 cut(s) 155, 176, 272
TfiI GAWTC 5 cut(s) 6, 578, 587, 843, 881
Tru1I TTAA 5 cut(s) 228, 456, 725, 918, 1081
Tru9I TTAA 5 cut(s) 228, 456, 725, 918, 1081
TscAI CASTG 3 cut(s) 663, 681, 706
TseFI GTSAC 5 cut(s) 253, 333, 476, 551, 1059
TseI GCWGC 4 cut(s) 395, 719, 923, 971
Tsp45I GTSAC 5 cut(s) 253, 333, 476, 551, 1059
TspDTI ATGAA 3 cut(s) 37, 52, 722
TspGWI ACGGA 4 cut(s) 154, 189, 357, 403
TspRI CASTG 3 cut(s) 663, 681, 706
XapI RAATTY 1 cut(s) 781
XceI RCATGY 2 cut(s) 305, 545
XhoI CTCGAG 1 cut(s) 612
XmnI GAANNNNTTC 1 cut(s) 263
XspI CTAG 3 cut(s) 206, 956, 1008
ZraI GACGTC 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.