AT2G32270

zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0009811
Physical Location & Seq
Forward (+)
13704068 .. 13706928
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G32270.1

Sequence Viewer

Length: 1020 bp
ATGAAGACTAAGAACGTGAAACTCTTATTCTTCTTCTTCTCCGTCTCCCTCCTTCTCATCGCCGTCGTCAACGCCGCCGAAGGCCATTCACATGGTGGACCAAAATGTGAATGCTCACACGAAGACGACCATGAAAACAAAGCCGGAGCTCGGAAATACAAGATCGCCGCAATTCCTACAGTTCTAATAGCCGGCATAATCGGAGTTCTTTTCCCTTTGTTAGGCAAAGTCTTCCCTTCTTTGCGTCCAGAAACATGTTTCTTCTTCGTCACGAAAGCTTTCGCAGCCGGAGTTATCTTGGCTACCGGATTTATGCATGTCTTGCCTGAGGCTTACGAGATGCTTAACTCTCCATGTTTGACATCTGAAGCATGGGAATTTCCGTTCACCGGATTTATTGCGATGATTGCTGCGATCTTGACGTTATCCGTTGATACATTTGCCACTTCGAGTTTCTATAAATCGCATTGCAAAGCGTCTAAGAGGGTCAGTGATGGAGAAACCGGCGAGTCCTCCGTTGACTCCGAGAAGGTCCAAATTCTCCGGACTAGAGTTATTGCACAGGTATTGGAGTTGGGAATAATAGTACACTCAGTGGTAATAGGAATATCACTAGGAGCTTCACAGAGCCCAGATGCTGCAAAAGCTCTGTTTATTGCCTTAATGTTTCATCAATGCTTCGAAGGTCTAGGCCTTGGTGGTTGTATTGCTCAGGGAAAATTCAAGTGTTTGTCAGTAACAATCATGTCGACGTTCTTCGCAATAACGACACCGATAGGAATCGTTGTGGGAATGGGAATAGCAAATTCTTACGATGAGTCTTCACCAACGGCTCTGATCGTTCAAGGAGTTTTGAACGCTGCATCCGCAGGCATTCTCATCTACATGTCTTTGGTTGACCTTCTCGCAGCAGATTTCACGCACCCTAAAATGCAATCCAATACTGGGCTTCAAATTATGGCCCATATTGCTCTCCTTCTTGGTGCTGGCCTCATGTCTCTATTGGCTAAATGGGCTTGA

Protein Analysis

339

Amino Acids

36.04

Weight (kDa)

6.78

Isoelectric Point (pI)

35.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 53 - 336 8.4e-79 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 749
AccIII TCCGGA 1 cut(s) 543
AciI CCGC 3 cut(s) 75, 168, 867
AcsI RAATTY 4 cut(s) 377, 537, 719, 805
AcuI CTGAAG 1 cut(s) 387
AdeI CACNNNGTG 2 cut(s) 95, 595
AfaI GTAC 1 cut(s) 588
AfiI CCNNNNNNNGG 4 cut(s) 150, 221, 389, 945
AflIII ACRYGT 2 cut(s) 254, 885
AgsI TTSAA 4 cut(s) 724, 845, 856, 953
AloI GAACNNNNNNTCC 4 cut(s) 368, 400, 848, 880
AluBI AGCT 4 cut(s) 149, 278, 620, 647
AluI AGCT 4 cut(s) 149, 278, 620, 647
Alw21I GWGCWC 1 cut(s) 151
Alw26I GTCTC 2 cut(s) 49, 1002
AlwNI CAGNNNCTG 1 cut(s) 638
Aor13HI TCCGGA 1 cut(s) 543
AoxI GGCC 4 cut(s) 82, 691, 960, 988
ApeKI GCWGC 5 cut(s) 284, 410, 638, 860, 908
ApoI RAATTY 4 cut(s) 377, 537, 719, 805
Asp700I GAANNNNTTC 1 cut(s) 278
AspS9I GGNCC 3 cut(s) 98, 532, 961
AsuHPI GGTGA 2 cut(s) 379, 816
AsuII TTCGAA 1 cut(s) 681
AvaII GGWCC 2 cut(s) 98, 532
AxyI CCTNAGG 1 cut(s) 327
BanII GRGCYC 2 cut(s) 151, 632
BbsI GAAGAC 4 cut(s) 11, 129, 223, 813
Bbv12I GWGCWC 1 cut(s) 151
BbvI GCAGC 5 cut(s) 296, 397, 625, 847, 920
BccI CCATC 1 cut(s) 488
BceAI ACGGC 2 cut(s) 47, 846
BcoDI GTCTC 2 cut(s) 49, 1002
BfaI CTAG 3 cut(s) 549, 614, 689
BfmI CTRYAG 1 cut(s) 177
BisI GCNGC 7 cut(s) 75, 168, 285, 411, 639, 861, 909
BlsI GCNGC 7 cut(s) 76, 169, 286, 412, 640, 862, 910
Bme18I GGWCC 2 cut(s) 98, 532
BmgT120I GGNCC 3 cut(s) 98, 532, 961
BmrI ACTGGG 1 cut(s) 954
BmsI GCATC 3 cut(s) 330, 625, 872
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 4 cut(s) 11, 129, 223, 813
Bpu10I CCTNAGC 1 cut(s) 711
Bpu14I TTCGAA 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 779
BsaJI CCNNGG 1 cut(s) 694
BsaWI WCCGGW 3 cut(s) 305, 389, 543
Bsc4I CCNNNNNNNGG 4 cut(s) 150, 221, 389, 945
Bse118I RCCGGY 2 cut(s) 191, 503
Bse1I ACTGG 1 cut(s) 949
Bse21I CCTNAGG 1 cut(s) 327
Bse3DI GCAATG 1 cut(s) 466
Bse8I GATNNNNATC 1 cut(s) 779
BseAI TCCGGA 1 cut(s) 543
BseDI CCNNGG 1 cut(s) 694
BseGI GGATG 1 cut(s) 863
BseJI GATNNNNATC 1 cut(s) 779
BseLI CCNNNNNNNGG 4 cut(s) 150, 221, 389, 945
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 3 cut(s) 318, 606, 725
BseNI ACTGG 1 cut(s) 949
BseXI GCAGC 5 cut(s) 296, 397, 625, 847, 920
BshFI GGCC 4 cut(s) 84, 693, 962, 990
BsiHKAI GWGCWC 1 cut(s) 151
BsiSI CCGG 7 cut(s) 144, 192, 288, 306, 390, 504, 544
BslI CCNNNNNNNGG 4 cut(s) 150, 221, 389, 945
BsmAI GTCTC 2 cut(s) 49, 1002
BsmBI CGTCTC 1 cut(s) 49
BsmI GAATGC 2 cut(s) 116, 873
BsnI GGCC 4 cut(s) 84, 693, 962, 990
Bsp119I TTCGAA 1 cut(s) 681
Bsp1286I GDGCHC 2 cut(s) 151, 632
Bsp13I TCCGGA 1 cut(s) 543
Bsp143I GATC 3 cut(s) 162, 414, 837
BspACI CCGC 3 cut(s) 75, 168, 867
BspANI GGCC 4 cut(s) 84, 693, 962, 990
BspCNI CTCAG 3 cut(s) 319, 605, 724
BspEI TCCGGA 1 cut(s) 543
BspT104I TTCGAA 1 cut(s) 681
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 2 cut(s) 191, 503
BsrI ACTGG 1 cut(s) 949
BssAI RCCGGY 2 cut(s) 191, 503
BssECI CCNNGG 1 cut(s) 694
BssMI GATC 3 cut(s) 162, 414, 837
BssT1I CCWWGG 1 cut(s) 694
Bst4CI ACNGT 1 cut(s) 181
BstAPI GCANNNNNTGC 1 cut(s) 322
BstBI TTCGAA 1 cut(s) 681
BstC8I GCNNGC 3 cut(s) 193, 871, 988
BstDEI CTNAG 5 cut(s) 9, 327, 480, 592, 711
BstENI CCTNNNNNAGG 1 cut(s) 219
BstF5I GGATG 1 cut(s) 863
BstKTI GATC 3 cut(s) 165, 417, 840
BstMAI GTCTC 2 cut(s) 49, 1002
BstMBI GATC 3 cut(s) 162, 414, 837
BstMWI GCNNNNNNNGC 7 cut(s) 284, 322, 407, 644, 866, 968, 1013
BstNSI RCATGY 3 cut(s) 258, 320, 889
BstSFI CTRYAG 1 cut(s) 177
BstV1I GCAGC 5 cut(s) 296, 397, 625, 847, 920
BstV2I GAAGAC 4 cut(s) 11, 129, 223, 813
BstXI CCANNNNNNTGG 1 cut(s) 92
Bsu36I CCTNAGG 1 cut(s) 327
BsuRI GGCC 4 cut(s) 84, 693, 962, 990
BtgZI GCGATG 2 cut(s) 43, 416
BtsCI GGATG 1 cut(s) 863
BtsIMutI CAGTG 2 cut(s) 496, 600
Cac8I GCNNGC 3 cut(s) 193, 871, 988
CaiI CAGNNNCTG 1 cut(s) 638
Cfr10I RCCGGY 2 cut(s) 191, 503
Cfr13I GGNCC 3 cut(s) 98, 532, 961
CseI GACGC 2 cut(s) 233, 465
Csp6I GTAC 1 cut(s) 587
CviAII CATG 9 cut(s) 92, 131, 255, 317, 354, 372, 745, 886, 994
CviQI GTAC 1 cut(s) 587
DdeI CTNAG 5 cut(s) 9, 327, 480, 592, 711
DpnI GATC 3 cut(s) 164, 416, 839
DpnII GATC 3 cut(s) 162, 414, 837
DraIII CACNNNGTG 2 cut(s) 95, 595
Ecl136II GAGCTC 1 cut(s) 149
Eco130I CCWWGG 1 cut(s) 694
Eco147I AGGCCT 1 cut(s) 693
Eco24I GRGCYC 2 cut(s) 151, 632
Eco47I GGWCC 2 cut(s) 98, 532
Eco53kI GAGCTC 1 cut(s) 149
Eco57I CTGAAG 1 cut(s) 387
Eco81I CCTNAGG 1 cut(s) 327
EcoICRI GAGCTC 1 cut(s) 149
EcoNI CCTNNNNNAGG 1 cut(s) 219
EcoT14I CCWWGG 1 cut(s) 694
EcoT22I ATGCAT 1 cut(s) 318
EcoT38I GRGCYC 2 cut(s) 151, 632
ErhI CCWWGG 1 cut(s) 694
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 9 cut(s) 95, 134, 258, 320, 357, 375, 748, 889, 997
FatI CATG 9 cut(s) 91, 130, 254, 316, 353, 371, 744, 885, 993
FblI GTMKAC 1 cut(s) 749
Fnu4HI GCNGC 7 cut(s) 75, 168, 285, 411, 639, 861, 909
FokI GGATG 1 cut(s) 850
FriOI GRGCYC 2 cut(s) 151, 632
Fsp4HI GCNGC 7 cut(s) 75, 168, 285, 411, 639, 861, 909
FspBI CTAG 3 cut(s) 549, 614, 689
GluI GCNGC 7 cut(s) 75, 168, 285, 411, 639, 861, 909
HaeIII GGCC 4 cut(s) 84, 693, 962, 990
HapII CCGG 7 cut(s) 144, 192, 288, 306, 390, 504, 544
HgaI GACGC 2 cut(s) 233, 465
Hin1II CATG 9 cut(s) 95, 134, 258, 320, 357, 375, 748, 889, 997
HincII GTYRAC 4 cut(s) 70, 520, 750, 898
HindII GTYRAC 4 cut(s) 70, 520, 750, 898
HindIII AAGCTT 1 cut(s) 276
HinfI GANTC 4 cut(s) 509, 521, 780, 818
HpaII CCGG 7 cut(s) 144, 192, 288, 306, 390, 504, 544
HphI GGTGA 2 cut(s) 379, 816
Hpy166II GTNNAC 7 cut(s) 70, 98, 387, 520, 589, 750, 898
Hpy188I TCNGA 5 cut(s) 153, 203, 367, 526, 837
Hpy188III TCNNGA 4 cut(s) 248, 271, 418, 544
Hpy8I GTNNAC 7 cut(s) 70, 98, 387, 520, 589, 750, 898
Hpy99I CGWCG 2 cut(s) 68, 754
HpyAV CCTTC 7 cut(s) 62, 74, 246, 523, 677, 911, 986
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4IV ACGT 3 cut(s) 15, 422, 752
HpyCH4V TGCA 6 cut(s) 316, 471, 560, 641, 863, 934
HpyF10VI GCNNNNNNNGC 7 cut(s) 284, 322, 407, 644, 866, 968, 1013
HpyF3I CTNAG 5 cut(s) 9, 327, 480, 592, 711
HpySE526I ACGT 3 cut(s) 15, 422, 752
Hsp92II CATG 9 cut(s) 95, 134, 258, 320, 357, 375, 748, 889, 997
Kpn2I TCCGGA 1 cut(s) 543
KroI GCCGGC 1 cut(s) 191
KroNI GCCGGC 1 cut(s) 193
Kzo9I GATC 3 cut(s) 162, 414, 837
LmnI GCTCC 2 cut(s) 146, 617
Lsp1109I GCAGC 5 cut(s) 296, 397, 625, 847, 920
LweI GCATC 3 cut(s) 330, 625, 872
MaeI CTAG 3 cut(s) 549, 614, 689
MaeII ACGT 3 cut(s) 15, 422, 752
MaeIII GTNAC 2 cut(s) 268, 736
MalI GATC 3 cut(s) 164, 416, 839
MboI GATC 3 cut(s) 162, 414, 837
MhlI GDGCHC 2 cut(s) 151, 632
MluCI AATT 6 cut(s) 171, 377, 537, 719, 805, 954
MlyI GAGTC 3 cut(s) 515, 518, 827
MnlI CCTC 5 cut(s) 59, 322, 477, 523, 1001
Mph1103I ATGCAT 1 cut(s) 318
MroI TCCGGA 1 cut(s) 543
MroNI GCCGGC 1 cut(s) 191
MroXI GAANNNNTTC 1 cut(s) 278
MseI TTAA 2 cut(s) 345, 662
MslI CAYNNNNRTG 2 cut(s) 90, 884
MspI CCGG 7 cut(s) 144, 192, 288, 306, 390, 504, 544
Mva1269I GAATGC 2 cut(s) 116, 873
MwoI GCNNNNNNNGC 7 cut(s) 284, 322, 407, 644, 866, 968, 1013
NaeI GCCGGC 1 cut(s) 193
NdeII GATC 3 cut(s) 162, 414, 837
NgoMIV GCCGGC 1 cut(s) 191
NlaIII CATG 9 cut(s) 95, 134, 258, 320, 357, 375, 748, 889, 997
NmuCI GTSAC 1 cut(s) 268
NsiI ATGCAT 1 cut(s) 318
NspI RCATGY 3 cut(s) 258, 320, 889
NspV TTCGAA 1 cut(s) 681
PceI AGGCCT 1 cut(s) 693
PciI ACATGT 2 cut(s) 254, 885
PctI GAATGC 2 cut(s) 116, 873
PdiI GCCGGC 1 cut(s) 193
PdmI GAANNNNTTC 1 cut(s) 278
PfeI GAWTC 1 cut(s) 780
PkrI GCNGC 7 cut(s) 76, 169, 286, 412, 640, 862, 910
PleI GAGTC 3 cut(s) 515, 517, 826
PpsI GAGTC 3 cut(s) 515, 517, 826
PscI ACATGT 2 cut(s) 254, 885
Psp124BI GAGCTC 1 cut(s) 151
PspPI GGNCC 3 cut(s) 98, 532, 961
PstNI CAGNNNCTG 1 cut(s) 638
RsaI GTAC 1 cut(s) 588
RsaNI GTAC 1 cut(s) 587
RseI CAYNNNNRTG 2 cut(s) 90, 884
SacI GAGCTC 1 cut(s) 151
SalI GTCGAC 1 cut(s) 748
SaqAI TTAA 2 cut(s) 345, 662
SatI GCNGC 7 cut(s) 75, 168, 285, 411, 639, 861, 909
Sau3AI GATC 3 cut(s) 162, 414, 837
Sau96I GGNCC 3 cut(s) 98, 532, 961
SchI GAGTC 3 cut(s) 515, 518, 827
SduI GDGCHC 2 cut(s) 151, 632
SfaNI GCATC 3 cut(s) 330, 625, 872
SfcI CTRYAG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 681
SinI GGWCC 2 cut(s) 98, 532
SmiMI CAYNNNNRTG 2 cut(s) 90, 884
Sse9I AATT 6 cut(s) 171, 377, 537, 719, 805, 954
SseBI AGGCCT 1 cut(s) 693
SsiI CCGC 3 cut(s) 75, 168, 867
SspMI CTAG 3 cut(s) 549, 614, 689
SstI GAGCTC 1 cut(s) 151
StuI AGGCCT 1 cut(s) 693
StyI CCWWGG 1 cut(s) 694
TaaI ACNGT 1 cut(s) 181
TaiI ACGT 3 cut(s) 18, 425, 755
TaqI TCGA 3 cut(s) 449, 681, 749
TasI AATT 6 cut(s) 171, 377, 537, 719, 805, 954
TatI WGTACW 1 cut(s) 586
TauI GCSGC 2 cut(s) 77, 170
TfiI GAWTC 1 cut(s) 780
Tru1I TTAA 2 cut(s) 345, 662
Tru9I TTAA 2 cut(s) 345, 662
TscAI CASTG 2 cut(s) 496, 600
TseFI GTSAC 1 cut(s) 268
TseI GCWGC 5 cut(s) 284, 410, 638, 860, 908
Tsp45I GTSAC 1 cut(s) 268
TspDTI ATGAA 3 cut(s) 17, 147, 659
TspGWI ACGGA 4 cut(s) 31, 372, 418, 505
TspRI CASTG 2 cut(s) 496, 600
VpaK11BI GGWCC 2 cut(s) 98, 532
XagI CCTNNNNNAGG 1 cut(s) 219
XapI RAATTY 4 cut(s) 377, 537, 719, 805
XceI RCATGY 3 cut(s) 258, 320, 889
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 278
XspI CTAG 3 cut(s) 549, 614, 689
Zsp2I ATGCAT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.