FvH4_6g39460

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0009811
Physical Location & Seq
Reverse (-)
31157752 .. 31160156
2405 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39460.t1

Sequence Viewer

Length: 1062 bp
ATGAAGGAGTTTCAGAAGAATGACCTCCTCTCCTTCTTCTGCATCCTCCTTTTACTCCCAGCCCTAGCTTCCGCAGAGTGTACGTGCGACGCCGAAGAAGAAGGCGGTAACAGAAGCGAAGCCCTAAAATACAAACTCGGCGCAATCGCTTCCATCCTCGTGGCCGGTGCAATCGGCGTTTGCATCCCAATTATAGGGAAAACGATTCCGTCATTGCAGCCGGAGAAGCCTATCTTCTTCATTATCAAGGCTTTTGCAGCCGGCGTTATATTGGCCACTGGATTCATCCACGTGCTTCCGGACGCTTTCGAGAGATTGACGTCGCCGTGTTTGAAAGAAAACCCATGGGCGAACTTTCCCTTCACCGGATTTGTGGCCATGATGGCTGCCATCGGAACCTTGATGGTAGATTCCATTGCAACTTCTTATTTCAACAGGTCTCACTTTAAGAAGGCTCAGAATCACGTTAACGGAGATGAGGAGAAAGTTGGAGAGCATGAGGGTCACGTACATGTTCATACACATGGGACTCATGGCCATTCTCATGGCTCGCTTGATACCAATTCCGCCGAGTCACAACTTCTTCGGCACAGAGTTATATCAAAGGTTTTGGAGTTGGGAATTGTGGTGCACTCGGTGATAATTGGAATTTCGTTGGGTGCTTCTGAAAGTCCAGCAACAGTTCGACCTCTTGTTGCTGCTTTGACTTTCCATCAATTTTTTGAAGGCATGGGACTTGGTGGTTGCATAGCTCAGGCGAGAGAGTCGCGATTAACAACTATAATCATGGTGTTGTTCTTCTCGCTTACAACTCCGGTTGGAATTGGGATTGGTATTGGAATATCAAGCAGATACAAGGAAAACAGTTCAACGGCTCTCATACTCGAAGGACTTTTTGATGCAGCATCAGCCGGGATCTTAATCTACATGGCTCTGGTTGATCTCCTAGCTGCTGATTTTATGAACCCGAAAATGCAGAAGAATGTAAAGCTTCAAGTTGGGGCTAATGTTTCACTTCTTTTTGGAGCTGGTTGCATGTCTCTAATAGCCAAATGGGCTTAA

Protein Analysis

354

Amino Acids

37.74

Weight (kDa)

6.53

Isoelectric Point (pI)

30.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 43 - 350 4.1e-83 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 323
AccII CGCG 1 cut(s) 769
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 3 cut(s) 72, 105, 567
AclWI GGATC 1 cut(s) 923
AcoI YGGCCR 4 cut(s) 162, 273, 375, 535
AcsI RAATTY 1 cut(s) 648
AcvI CACGTG 1 cut(s) 292
AcyI GRCGYC 2 cut(s) 90, 320
AdeI CACNNNGTG 1 cut(s) 637
AfaI GTAC 2 cut(s) 82, 510
AfiI CCNNNNNNNGG 2 cut(s) 194, 365
AflIII ACRYGT 1 cut(s) 511
AgsI TTSAA 5 cut(s) 334, 433, 725, 870, 995
AluBI AGCT 5 cut(s) 68, 752, 950, 991, 1028
AluI AGCT 5 cut(s) 68, 752, 950, 991, 1028
Alw21I GWGCWC 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 444, 1044
Alw44I GTGCAC 1 cut(s) 629
AlwI GGATC 1 cut(s) 923
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 4 cut(s) 162, 273, 375, 535
ApaLI GTGCAC 1 cut(s) 629
ApeKI GCWGC 6 cut(s) 217, 257, 386, 698, 902, 950
ApoI RAATTY 1 cut(s) 648
AspLEI GCGC 1 cut(s) 143
AsuC2I CCSGG 1 cut(s) 913
AsuHPI GGTGA 2 cut(s) 355, 649
BaeGI GKGCMC 1 cut(s) 633
BalI TGGCCA 3 cut(s) 275, 377, 537
BauI CACGAG 1 cut(s) 158
BbrPI CACGTG 1 cut(s) 292
Bbv12I GWGCWC 1 cut(s) 633
BbvI GCAGC 6 cut(s) 229, 269, 373, 685, 914, 937
BccI CCATC 5 cut(s) 161, 376, 397, 398, 720
BceAI ACGGC 2 cut(s) 310, 888
BcnI CCSGG 1 cut(s) 913
BcoDI GTCTC 2 cut(s) 444, 1044
BfaI CTAG 2 cut(s) 65, 947
BglI GCCNNNNNGGC 2 cut(s) 383, 1055
BisI GCNGC 6 cut(s) 218, 258, 387, 699, 903, 951
BlsI GCNGC 6 cut(s) 219, 259, 388, 700, 904, 952
Bme1390I CCNGG 1 cut(s) 913
BmiI GGNNCC 1 cut(s) 397
BmrFI CCNGG 1 cut(s) 913
BmsI GCATC 4 cut(s) 51, 192, 889, 914
Bpu10I CCTNAGC 1 cut(s) 753
BpuMI CCSGG 1 cut(s) 913
BsaAI YACGTR 3 cut(s) 84, 292, 508
BsaBI GATNNNNATC 1 cut(s) 920
BsaHI GRCGYC 2 cut(s) 90, 320
BsaI GGTCTC 1 cut(s) 444
BsaJI CCNNGG 1 cut(s) 344
BsaWI WCCGGW 3 cut(s) 298, 365, 814
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 2 cut(s) 194, 365
Bse118I RCCGGY 2 cut(s) 164, 260
Bse1I ACTGG 1 cut(s) 283
Bse3DI GCAATG 2 cut(s) 212, 414
Bse8I GATNNNNATC 1 cut(s) 920
BseAI TCCGGA 1 cut(s) 298
BseDI CCNNGG 1 cut(s) 344
BseGI GGATG 4 cut(s) 42, 153, 183, 285
BseJI GATNNNNATC 1 cut(s) 920
BseLI CCNNNNNNNGG 2 cut(s) 194, 365
BseMI GCAATG 2 cut(s) 212, 414
BseMII CTCAG 2 cut(s) 470, 767
BseNI ACTGG 1 cut(s) 283
BseRI GAGGAG 2 cut(s) 17, 494
BseSI GKGCMC 1 cut(s) 633
BseXI GCAGC 6 cut(s) 229, 269, 373, 685, 914, 937
BseYI CCCAGC 1 cut(s) 58
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 4 cut(s) 164, 275, 377, 537
BsiHKAI GWGCWC 1 cut(s) 633
BsiSI CCGG 7 cut(s) 165, 221, 261, 299, 366, 815, 912
BslFI GGGAC 2 cut(s) 541, 747
BslI CCNNNNNNNGG 2 cut(s) 194, 365
BsmAI GTCTC 2 cut(s) 444, 1044
BsmFI GGGAC 2 cut(s) 541, 747
BsnI GGCC 4 cut(s) 164, 275, 377, 537
Bso31I GGTCTC 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 633
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 2 cut(s) 915, 940
Bsp19I CCATGG 1 cut(s) 344
Bsp68I TCGCGA 1 cut(s) 769
BspACI CCGC 3 cut(s) 72, 105, 567
BspANI GGCC 4 cut(s) 164, 275, 377, 537
BspCNI CTCAG 2 cut(s) 469, 766
BspEI TCCGGA 1 cut(s) 298
BspFNI CGCG 1 cut(s) 769
BspLI GGNNCC 1 cut(s) 397
BspPI GGATC 1 cut(s) 923
BspTNI GGTCTC 1 cut(s) 444
BsrDI GCAATG 2 cut(s) 212, 414
BsrFI RCCGGY 2 cut(s) 164, 260
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 2 cut(s) 164, 260
BssECI CCNNGG 1 cut(s) 344
BssMI GATC 2 cut(s) 915, 940
BssNI GRCGYC 2 cut(s) 90, 320
BssSI CACGAG 1 cut(s) 158
BssT1I CCWWGG 1 cut(s) 344
Bst2BI CACGAG 1 cut(s) 158
Bst4CI ACNGT 2 cut(s) 682, 866
BstACI GRCGYC 2 cut(s) 90, 320
BstBAI YACGTR 3 cut(s) 84, 292, 508
BstC8I GCNNGC 2 cut(s) 262, 551
BstDEI CTNAG 2 cut(s) 456, 753
BstDSI CCRYGG 1 cut(s) 344
BstF5I GGATG 4 cut(s) 42, 153, 183, 285
BstFNI CGCG 1 cut(s) 769
BstHHI GCGC 1 cut(s) 143
BstKTI GATC 2 cut(s) 918, 943
BstMAI GTCTC 2 cut(s) 444, 1044
BstMBI GATC 2 cut(s) 915, 940
BstMWI GCNNNNNNNGC 5 cut(s) 226, 257, 383, 908, 1055
BstNSI RCATGY 2 cut(s) 515, 1039
BstSCI CCNGG 1 cut(s) 911
BstSLI GKGCMC 1 cut(s) 633
BstUI CGCG 1 cut(s) 769
BstV1I GCAGC 6 cut(s) 229, 269, 373, 685, 914, 937
BstX2I RGATCY 1 cut(s) 915
BstXI CCANNNNNNTGG 2 cut(s) 160, 545
BstYI RGATCY 1 cut(s) 915
BsuRI GGCC 4 cut(s) 164, 275, 377, 537
BtgI CCRYGG 1 cut(s) 344
BtsCI GGATG 4 cut(s) 42, 153, 183, 285
BtsIMutI CAGTG 1 cut(s) 276
BtuMI TCGCGA 1 cut(s) 769
Cac8I GCNNGC 2 cut(s) 262, 551
CfoI GCGC 1 cut(s) 143
Cfr10I RCCGGY 2 cut(s) 164, 260
CseI GACGC 2 cut(s) 98, 311
Csp6I GTAC 2 cut(s) 81, 509
CviQI GTAC 2 cut(s) 81, 509
DdeI CTNAG 2 cut(s) 456, 753
DpnI GATC 2 cut(s) 917, 942
DpnII GATC 2 cut(s) 915, 940
DraIII CACNNNGTG 1 cut(s) 637
EaeI YGGCCR 4 cut(s) 162, 273, 375, 535
EciI GGCGGA 1 cut(s) 556
Eco130I CCWWGG 1 cut(s) 344
Eco31I GGTCTC 1 cut(s) 444
Eco72I CACGTG 1 cut(s) 292
EcoT14I CCWWGG 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 344
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 2 cut(s) 541, 747
Fnu4HI GCNGC 6 cut(s) 218, 258, 387, 699, 903, 951
FokI GGATG 4 cut(s) 29, 140, 170, 272
Fsp4HI GCNGC 6 cut(s) 218, 258, 387, 699, 903, 951
FspBI CTAG 2 cut(s) 65, 947
GlaI GCGC 1 cut(s) 142
GluI GCNGC 6 cut(s) 218, 258, 387, 699, 903, 951
GsaI CCCAGC 1 cut(s) 62
HaeIII GGCC 4 cut(s) 164, 275, 377, 537
HapII CCGG 7 cut(s) 165, 221, 261, 299, 366, 815, 912
HgaI GACGC 2 cut(s) 98, 311
HhaI GCGC 1 cut(s) 143
Hin1I GRCGYC 2 cut(s) 90, 320
Hin6I GCGC 1 cut(s) 141
HinP1I GCGC 1 cut(s) 141
HincII GTYRAC 1 cut(s) 469
HindII GTYRAC 1 cut(s) 469
HindIII AAGCTT 1 cut(s) 989
HinfI GANTC 7 cut(s) 205, 282, 410, 460, 529, 572, 764
HpaI GTTAAC 1 cut(s) 469
HpaII CCGG 7 cut(s) 165, 221, 261, 299, 366, 815, 912
HphI GGTGA 2 cut(s) 355, 649
Hpy166II GTNNAC 3 cut(s) 81, 469, 631
Hpy188I TCNGA 4 cut(s) 15, 395, 459, 667
Hpy188III TCNNGA 3 cut(s) 299, 310, 768
Hpy8I GTNNAC 3 cut(s) 81, 469, 631
Hpy99I CGWCG 2 cut(s) 92, 325
HpyAV CCTTC 6 cut(s) 43, 95, 370, 445, 719, 881
HpyCH4III ACNGT 2 cut(s) 682, 866
HpyCH4IV ACGT 5 cut(s) 83, 291, 320, 465, 507
HpyF10VI GCNNNNNNNGC 5 cut(s) 226, 257, 383, 908, 1055
HpyF3I CTNAG 2 cut(s) 456, 753
HpySE526I ACGT 5 cut(s) 83, 291, 320, 465, 507
Hsp92I GRCGYC 2 cut(s) 90, 320
HspAI GCGC 1 cut(s) 141
Kpn2I TCCGGA 1 cut(s) 298
KroI GCCGGC 1 cut(s) 260
KroNI GCCGGC 1 cut(s) 262
KspAI GTTAAC 1 cut(s) 469
Kzo9I GATC 2 cut(s) 915, 940
LmnI GCTCC 1 cut(s) 1025
Lsp1109I GCAGC 6 cut(s) 229, 269, 373, 685, 914, 937
LweI GCATC 4 cut(s) 51, 192, 889, 914
MaeI CTAG 2 cut(s) 65, 947
MaeII ACGT 5 cut(s) 83, 291, 320, 465, 507
MaeIII GTNAC 3 cut(s) 107, 503, 573
MalI GATC 2 cut(s) 917, 942
MboI GATC 2 cut(s) 915, 940
MboII GAAGA 9 cut(s) 28, 28, 107, 110, 226, 229, 575, 790, 991
MflI RGATCY 1 cut(s) 915
MhlI GDGCHC 1 cut(s) 633
MlsI TGGCCA 3 cut(s) 275, 377, 537
MluCI AATT 7 cut(s) 189, 562, 621, 642, 648, 716, 822
MluNI TGGCCA 3 cut(s) 275, 377, 537
MlyI GAGTC 3 cut(s) 523, 581, 773
MmeI TCCRAC 2 cut(s) 469, 799
MnlI CCTC 7 cut(s) 35, 38, 56, 167, 472, 493, 699
Mox20I TGGCCA 3 cut(s) 275, 377, 537
MroI TCCGGA 1 cut(s) 298
MroNI GCCGGC 1 cut(s) 260
MscI TGGCCA 3 cut(s) 275, 377, 537
MseI TTAA 5 cut(s) 447, 468, 773, 920, 1060
MslI CAYNNNNRTG 5 cut(s) 158, 290, 510, 522, 543
Msp20I TGGCCA 3 cut(s) 275, 377, 537
MspI CCGG 7 cut(s) 165, 221, 261, 299, 366, 815, 912
MspR9I CCNGG 1 cut(s) 913
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 5 cut(s) 226, 257, 383, 908, 1055
NaeI GCCGGC 1 cut(s) 262
NciI CCSGG 1 cut(s) 913
NcoI CCATGG 1 cut(s) 344
NdeII GATC 2 cut(s) 915, 940
NgoMIV GCCGGC 1 cut(s) 260
NlaIV GGNNCC 1 cut(s) 397
NmeAIII GCCGAG 2 cut(s) 117, 595
NmuCI GTSAC 2 cut(s) 503, 573
NruI TCGCGA 1 cut(s) 769
NspI RCATGY 2 cut(s) 515, 1039
PciI ACATGT 1 cut(s) 511
PdiI GCCGGC 1 cut(s) 262
PfeI GAWTC 4 cut(s) 205, 282, 410, 460
PkrI GCNGC 6 cut(s) 219, 259, 388, 700, 904, 952
PleI GAGTC 3 cut(s) 523, 580, 772
PmaCI CACGTG 1 cut(s) 292
PmlI CACGTG 1 cut(s) 292
PpsI GAGTC 3 cut(s) 523, 580, 772
Ppu21I YACGTR 3 cut(s) 84, 292, 508
PscI ACATGT 1 cut(s) 511
PspCI CACGTG 1 cut(s) 292
PspFI CCCAGC 1 cut(s) 58
PspN4I GGNNCC 1 cut(s) 397
PsuI RGATCY 1 cut(s) 915
RruI TCGCGA 1 cut(s) 769
RsaI GTAC 2 cut(s) 82, 510
RsaNI GTAC 2 cut(s) 81, 509
RseI CAYNNNNRTG 5 cut(s) 158, 290, 510, 522, 543
SaqAI TTAA 5 cut(s) 447, 468, 773, 920, 1060
SatI GCNGC 6 cut(s) 218, 258, 387, 699, 903, 951
Sau3AI GATC 2 cut(s) 915, 940
SchI GAGTC 3 cut(s) 523, 581, 773
ScrFI CCNGG 1 cut(s) 913
SduI GDGCHC 1 cut(s) 633
SfaNI GCATC 4 cut(s) 51, 192, 889, 914
SmiMI CAYNNNNRTG 5 cut(s) 158, 290, 510, 522, 543
Sse9I AATT 7 cut(s) 189, 562, 621, 642, 648, 716, 822
SsiI CCGC 3 cut(s) 72, 105, 567
SspMI CTAG 2 cut(s) 65, 947
StyD4I CCNGG 1 cut(s) 911
StyI CCWWGG 1 cut(s) 344
TaaI ACNGT 2 cut(s) 682, 866
TaiI ACGT 5 cut(s) 86, 294, 323, 468, 510
TaqI TCGA 3 cut(s) 309, 685, 885
TasI AATT 7 cut(s) 189, 562, 621, 642, 648, 716, 822
TfiI GAWTC 4 cut(s) 205, 282, 410, 460
Tru1I TTAA 5 cut(s) 447, 468, 773, 920, 1060
Tru9I TTAA 5 cut(s) 447, 468, 773, 920, 1060
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 2 cut(s) 503, 573
TseI GCWGC 6 cut(s) 217, 257, 386, 698, 902, 950
Tsp45I GTSAC 2 cut(s) 503, 573
TspDTI ATGAA 5 cut(s) 17, 229, 274, 506, 977
TspGWI ACGGA 2 cut(s) 198, 486
TspRI CASTG 1 cut(s) 283
VneI GTGCAC 1 cut(s) 629
XapI RAATTY 1 cut(s) 648
XceI RCATGY 2 cut(s) 515, 1039
XspI CTAG 2 cut(s) 65, 947
ZraI GACGTC 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.