Rroxscaffold_2G00095000

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009811
Physical Location & Seq
Forward (+)
16327983 .. 16330182
2200 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00095000.1

Sequence Viewer

Length: 1065 bp
ATGAACAAGTTTCAGAAGAAAGGCCTCCTCTCGTTTTTCTGCCTCCTCCTTTTACTCCCAATCTTTGCTTCCGCTGAGTGTACGTGCGACGCCGAAGATGAAGGTGATAGCAACAAAAGCAAAGCTCTCAAATACAAACTCGGCGCATTCGCTTCCATACTCGTCGCCGGTGCAGTCGGTGTTTGTATTCCAATTCTAGGGAAGACAATTCCGGCTCTGCAACCGGACAAGAATATCTTCTTCATCGTCAAGGCTTTCGCCGCCGGCGTCATATTGGCCACCGGATTCATCCACGTGCTTCCGGACGCTTTCGACAACTTGACGTCGCCATGCCTGAAAGAAAACCCATGGGGCAAGTTTCCCTTCACCGGATTTGTGGCCATGATGGCGGCCATCGGAACTTTGATGGTGGATTCCATTGCTACTTCTTATTTCAGCGGGTCTCACTTCAATACGGCTCAGAATCAGATTAATGGAGATGAGGAGAAAGAGGGACAGCACGAGGGGCACGTACATGTTCATACACATGGCACTCATGGTCATTCTCATGGTTCGCTTGATACGAATTCGGCTGAATCACAACTTCTTCGGCATAGAGTGATTTCAAAGGTGTTGGAGTTGGGAATTGTGGTGCACTCGGTGATTATTGGAATTTCATTGGGTGCTTCTGAAAGTCCTGAAACAATTCGACCTCTTGTAGCTGCTTTGACTTTCCATCAATTTTTTGAAGGCATGGGACTTGGTGGTTGCATAGCTCAGGCGAGAGAATCGCGAATAACAACTATAATCATGGTGCTGTTCTTCTCCCTAACAACCCCTGTTGGAATCGCGATTGGAATTGGAGTATCAAGTAATTACAAGGAAAACAGTCCACCTGCTCTCATACTTGAAGGAACTTTTAATGCGGCATCAGCTGGGATCTTAATCTACATGTCTCTGGTCGATCTGCTAGCGGCTGATTTTATGAACCCGAAAATGCAGAAGAATGTGAAGCTTCAAATTGGGGCTAATGCTTCGCTTCTTTTTGGAGCTGGTTGCATGTCTCTATTGGCCAAATGGGCTTAA

Protein Analysis

354

Amino Acids

37.69

Weight (kDa)

6.49

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 44 - 351 1.7e-81 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 883
AatII GACGTC 1 cut(s) 326
Acc36I ACCTGC 1 cut(s) 883
AccII CGCG 2 cut(s) 772, 830
AccIII TCCGGA 1 cut(s) 301
AciI CCGC 6 cut(s) 72, 261, 389, 438, 905, 953
AclWI GGATC 1 cut(s) 926
AcoI YGGCCR 4 cut(s) 276, 378, 390, 1050
AcsI RAATTY 2 cut(s) 565, 651
AcvI CACGTG 1 cut(s) 295
AcyI GRCGYC 3 cut(s) 90, 267, 323
AdeI CACNNNGTG 1 cut(s) 640
AfaI GTAC 2 cut(s) 82, 513
AfiI CCNNNNNNNGG 2 cut(s) 197, 368
AflIII ACRYGT 2 cut(s) 514, 930
AgsI TTSAA 5 cut(s) 451, 606, 728, 890, 998
AjuI GAANNNNNNNTTGG 2 cut(s) 52, 84
AluBI AGCT 6 cut(s) 125, 701, 755, 914, 994, 1031
AluI AGCT 6 cut(s) 125, 701, 755, 914, 994, 1031
Alw21I GWGCWC 1 cut(s) 636
Alw26I GTCTC 3 cut(s) 447, 939, 1047
Alw44I GTGCAC 1 cut(s) 632
AlwI GGATC 1 cut(s) 926
Aor13HI TCCGGA 1 cut(s) 301
AoxI GGCC 5 cut(s) 22, 276, 378, 390, 1050
ApaLI GTGCAC 1 cut(s) 632
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 2 cut(s) 565, 651
AseI ATTAAT 1 cut(s) 471
Asp700I GAANNNNTTC 2 cut(s) 236, 684
AspLEI GCGC 1 cut(s) 146
AsuHPI GGTGA 3 cut(s) 116, 358, 652
AsuNHI GCTAGC 1 cut(s) 949
BaeGI GKGCMC 2 cut(s) 510, 636
BalI TGGCCA 3 cut(s) 278, 380, 1052
BauI CACGAG 1 cut(s) 500
BbrPI CACGTG 1 cut(s) 295
BbsI GAAGAC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 636
BbvI GCAGC 1 cut(s) 688
BccI CCATC 4 cut(s) 379, 400, 401, 723
BceAI ACGGC 1 cut(s) 471
BcoDI GTCTC 3 cut(s) 447, 939, 1047
BfaI CTAG 2 cut(s) 197, 950
BfuAI ACCTGC 1 cut(s) 883
BglI GCCNNNNNGGC 2 cut(s) 386, 1058
BisI GCNGC 5 cut(s) 261, 390, 702, 906, 954
BlsI GCNGC 5 cut(s) 262, 391, 703, 907, 955
BmsI GCATC 1 cut(s) 917
BmtI GCTAGC 1 cut(s) 953
BpiI GAAGAC 1 cut(s) 209
Bpu10I CCTNAGC 1 cut(s) 756
BsaAI YACGTR 3 cut(s) 84, 295, 511
BsaBI GATNNNNATC 1 cut(s) 923
BsaHI GRCGYC 3 cut(s) 90, 267, 323
BsaI GGTCTC 1 cut(s) 447
BsaJI CCNNGG 1 cut(s) 347
BsaWI WCCGGW 4 cut(s) 223, 281, 301, 368
Bsc4I CCNNNNNNNGG 2 cut(s) 197, 368
Bse118I RCCGGY 2 cut(s) 167, 263
Bse3DI GCAATG 1 cut(s) 417
Bse8I GATNNNNATC 1 cut(s) 923
BseAI TCCGGA 1 cut(s) 301
BseDI CCNNGG 1 cut(s) 347
BseGI GGATG 1 cut(s) 288
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 2 cut(s) 197, 368
BseMI GCAATG 1 cut(s) 417
BseMII CTCAG 3 cut(s) 66, 473, 770
BseRI GAGGAG 3 cut(s) 17, 35, 497
BseSI GKGCMC 2 cut(s) 510, 636
BseXI GCAGC 1 cut(s) 688
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 192
Bsh1236I CGCG 2 cut(s) 772, 830
BshFI GGCC 5 cut(s) 24, 278, 380, 392, 1052
BsiHKAI GWGCWC 1 cut(s) 636
BsiSI CCGG 7 cut(s) 168, 212, 224, 264, 282, 302, 369
BslFI GGGAC 2 cut(s) 507, 750
BslI CCNNNNNNNGG 2 cut(s) 197, 368
BsmAI GTCTC 3 cut(s) 447, 939, 1047
BsmFI GGGAC 2 cut(s) 507, 750
BsmI GAATGC 1 cut(s) 146
BsnI GGCC 5 cut(s) 24, 278, 380, 392, 1052
Bso31I GGTCTC 1 cut(s) 447
Bsp1286I GDGCHC 2 cut(s) 510, 636
Bsp13I TCCGGA 1 cut(s) 301
Bsp143I GATC 2 cut(s) 918, 943
Bsp19I CCATGG 1 cut(s) 347
Bsp68I TCGCGA 2 cut(s) 772, 830
BspACI CCGC 6 cut(s) 72, 261, 389, 438, 905, 953
BspANI GGCC 5 cut(s) 24, 278, 380, 392, 1052
BspCNI CTCAG 3 cut(s) 67, 472, 769
BspEI TCCGGA 1 cut(s) 301
BspFNI CGCG 2 cut(s) 772, 830
BspMI ACCTGC 1 cut(s) 883
BspOI GCTAGC 1 cut(s) 953
BspPI GGATC 1 cut(s) 926
BspTNI GGTCTC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 417
BsrFI RCCGGY 2 cut(s) 167, 263
BssAI RCCGGY 2 cut(s) 167, 263
BssECI CCNNGG 1 cut(s) 347
BssMI GATC 2 cut(s) 918, 943
BssNI GRCGYC 3 cut(s) 90, 267, 323
BssSI CACGAG 1 cut(s) 500
BssT1I CCWWGG 1 cut(s) 347
Bst2BI CACGAG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 869
BstACI GRCGYC 3 cut(s) 90, 267, 323
BstBAI YACGTR 3 cut(s) 84, 295, 511
BstC8I GCNNGC 2 cut(s) 265, 951
BstDEI CTNAG 3 cut(s) 75, 459, 756
BstDSI CCRYGG 1 cut(s) 347
BstF5I GGATG 1 cut(s) 288
BstFNI CGCG 2 cut(s) 772, 830
BstHHI GCGC 1 cut(s) 146
BstKTI GATC 2 cut(s) 921, 946
BstMAI GTCTC 3 cut(s) 447, 939, 1047
BstMBI GATC 2 cut(s) 918, 943
BstMWI GCNNNNNNNGC 6 cut(s) 117, 260, 386, 505, 911, 1058
BstNSI RCATGY 3 cut(s) 518, 934, 1042
BstSLI GKGCMC 2 cut(s) 510, 636
BstUI CGCG 2 cut(s) 772, 830
BstV1I GCAGC 1 cut(s) 688
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 1 cut(s) 918
BstYI RGATCY 1 cut(s) 918
BsuRI GGCC 5 cut(s) 24, 278, 380, 392, 1052
BtgI CCRYGG 1 cut(s) 347
BtsCI GGATG 1 cut(s) 288
BtuMI TCGCGA 2 cut(s) 772, 830
BveI ACCTGC 1 cut(s) 883
Cac8I GCNNGC 2 cut(s) 265, 951
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 2 cut(s) 167, 263
CseI GACGC 3 cut(s) 98, 256, 314
Csp6I GTAC 2 cut(s) 81, 512
CviQI GTAC 2 cut(s) 81, 512
DdeI CTNAG 3 cut(s) 75, 459, 756
DpnI GATC 2 cut(s) 920, 945
DpnII GATC 2 cut(s) 918, 943
DraIII CACNNNGTG 1 cut(s) 640
EaeI YGGCCR 4 cut(s) 276, 378, 390, 1050
Eco130I CCWWGG 1 cut(s) 347
Eco147I AGGCCT 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 447
Eco72I CACGTG 1 cut(s) 295
EcoRI GAATTC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 347
FalI AAGNNNNNCTT 4 cut(s) 221, 253, 347, 379
FaqI GGGAC 2 cut(s) 507, 750
FauI CCCGC 1 cut(s) 431
Fnu4HI GCNGC 5 cut(s) 261, 390, 702, 906, 954
FokI GGATG 1 cut(s) 275
Fsp4HI GCNGC 5 cut(s) 261, 390, 702, 906, 954
FspBI CTAG 2 cut(s) 197, 950
GlaI GCGC 1 cut(s) 145
GluI GCNGC 5 cut(s) 261, 390, 702, 906, 954
GsaI CCCAGC 1 cut(s) 918
HaeIII GGCC 5 cut(s) 24, 278, 380, 392, 1052
HapII CCGG 7 cut(s) 168, 212, 224, 264, 282, 302, 369
HgaI GACGC 3 cut(s) 98, 256, 314
HhaI GCGC 1 cut(s) 146
Hin1I GRCGYC 3 cut(s) 90, 267, 323
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 992
HinfI GANTC 6 cut(s) 285, 413, 463, 575, 767, 825
HpaII CCGG 7 cut(s) 168, 212, 224, 264, 282, 302, 369
HphI GGTGA 3 cut(s) 116, 358, 652
Hpy166II GTNNAC 3 cut(s) 81, 634, 872
Hpy188I TCNGA 5 cut(s) 15, 398, 462, 468, 670
Hpy188III TCNNGA 4 cut(s) 302, 677, 771, 829
Hpy8I GTNNAC 3 cut(s) 81, 634, 872
Hpy99I CGWCG 3 cut(s) 92, 167, 328
HpyAV CCTTC 4 cut(s) 95, 373, 722, 884
HpyCH4III ACNGT 1 cut(s) 869
HpyCH4IV ACGT 4 cut(s) 83, 294, 323, 510
HpyCH4V TGCA 6 cut(s) 173, 220, 634, 750, 979, 1038
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 260, 386, 505, 911, 1058
HpyF3I CTNAG 3 cut(s) 75, 459, 756
HpySE526I ACGT 4 cut(s) 83, 294, 323, 510
Hsp92I GRCGYC 3 cut(s) 90, 267, 323
HspAI GCGC 1 cut(s) 144
Kpn2I TCCGGA 1 cut(s) 301
KroI GCCGGC 1 cut(s) 263
KroNI GCCGGC 1 cut(s) 265
Kzo9I GATC 2 cut(s) 918, 943
LmnI GCTCC 1 cut(s) 1028
Lsp1109I GCAGC 1 cut(s) 688
LweI GCATC 1 cut(s) 917
MaeI CTAG 2 cut(s) 197, 950
MaeII ACGT 4 cut(s) 83, 294, 323, 510
MalI GATC 2 cut(s) 920, 945
MboI GATC 2 cut(s) 918, 943
MboII GAAGA 8 cut(s) 28, 107, 214, 229, 232, 578, 793, 994
MflI RGATCY 1 cut(s) 918
MhlI GDGCHC 2 cut(s) 510, 636
MlsI TGGCCA 3 cut(s) 278, 380, 1052
MluNI TGGCCA 3 cut(s) 278, 380, 1052
MmeI TCCRAC 2 cut(s) 594, 802
MnlI CCTC 8 cut(s) 35, 38, 53, 56, 475, 484, 496, 702
Mox20I TGGCCA 3 cut(s) 278, 380, 1052
MreI CGCCGGCG 1 cut(s) 263
MroI TCCGGA 1 cut(s) 301
MroNI GCCGGC 1 cut(s) 263
MroXI GAANNNNTTC 2 cut(s) 236, 684
MscI TGGCCA 3 cut(s) 278, 380, 1052
MseI TTAA 4 cut(s) 471, 900, 923, 1063
MslI CAYNNNNRTG 4 cut(s) 293, 513, 525, 546
Msp20I TGGCCA 3 cut(s) 278, 380, 1052
MspA1I CMGCKG 3 cut(s) 74, 438, 914
MspI CCGG 7 cut(s) 168, 212, 224, 264, 282, 302, 369
Mva1269I GAATGC 1 cut(s) 146
MvnI CGCG 2 cut(s) 772, 830
MwoI GCNNNNNNNGC 6 cut(s) 117, 260, 386, 505, 911, 1058
NaeI GCCGGC 1 cut(s) 265
NcoI CCATGG 1 cut(s) 347
NdeII GATC 2 cut(s) 918, 943
NgoMIV GCCGGC 1 cut(s) 263
NheI GCTAGC 1 cut(s) 949
NmeAIII GCCGAG 1 cut(s) 120
NruI TCGCGA 2 cut(s) 772, 830
NspI RCATGY 3 cut(s) 518, 934, 1042
PaqCI CACCTGC 1 cut(s) 883
PceI AGGCCT 1 cut(s) 24
PciI ACATGT 2 cut(s) 514, 930
PcsI WCGNNNNNNNCGW 3 cut(s) 264, 507, 560
PctI GAATGC 1 cut(s) 146
PdiI GCCGGC 1 cut(s) 265
PdmI GAANNNNTTC 2 cut(s) 236, 684
PfeI GAWTC 6 cut(s) 285, 413, 463, 575, 767, 825
PkrI GCNGC 5 cut(s) 262, 391, 703, 907, 955
PmaCI CACGTG 1 cut(s) 295
PmlI CACGTG 1 cut(s) 295
Ppu21I YACGTR 3 cut(s) 84, 295, 511
PscI ACATGT 2 cut(s) 514, 930
PshBI ATTAAT 1 cut(s) 471
PspCI CACGTG 1 cut(s) 295
PspFI CCCAGC 1 cut(s) 914
PsuI RGATCY 1 cut(s) 918
PvuII CAGCTG 1 cut(s) 914
RruI TCGCGA 2 cut(s) 772, 830
RsaI GTAC 2 cut(s) 82, 513
RsaNI GTAC 2 cut(s) 81, 512
RseI CAYNNNNRTG 4 cut(s) 293, 513, 525, 546
SaqAI TTAA 4 cut(s) 471, 900, 923, 1063
SatI GCNGC 5 cut(s) 261, 390, 702, 906, 954
Sau3AI GATC 2 cut(s) 918, 943
SduI GDGCHC 2 cut(s) 510, 636
SfaNI GCATC 1 cut(s) 917
SgrAI CRCCGGYG 2 cut(s) 167, 263
SmiMI CAYNNNNRTG 4 cut(s) 293, 513, 525, 546
SseBI AGGCCT 1 cut(s) 24
SsiI CCGC 6 cut(s) 72, 261, 389, 438, 905, 953
SspMI CTAG 2 cut(s) 197, 950
StuI AGGCCT 1 cut(s) 24
StyI CCWWGG 1 cut(s) 347
TaaI ACNGT 1 cut(s) 869
TaiI ACGT 4 cut(s) 86, 297, 326, 513
TaqI TCGA 3 cut(s) 312, 688, 942
TauI GCSGC 4 cut(s) 263, 392, 908, 956
TfiI GAWTC 6 cut(s) 285, 413, 463, 575, 767, 825
Tru1I TTAA 4 cut(s) 471, 900, 923, 1063
Tru9I TTAA 4 cut(s) 471, 900, 923, 1063
TseI GCWGC 1 cut(s) 701
TspDTI ATGAA 7 cut(s) 17, 114, 232, 277, 509, 645, 980
VneI GTGCAC 1 cut(s) 632
VspI ATTAAT 1 cut(s) 471
XapI RAATTY 2 cut(s) 565, 651
XceI RCATGY 3 cut(s) 518, 934, 1042
XmnI GAANNNNTTC 2 cut(s) 236, 684
XspI CTAG 2 cut(s) 197, 950
ZraI GACGTC 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.