MD09G1140300.v1.1

Zinc transporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0009811
Physical Location & Seq
Forward (+)
10902562 .. 10904224
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1140300.v1.1.491

Sequence Viewer

Length: 1056 bp
ATGAGCAAACTTCGACAGCATAGTCGTTCGGCATTCTTTTGCCTCCTCTTCATACTCCCCACCTTAGTTTTCGGAGAGTGCACCTGTGAAAAAGGAGAAGGGGATGGGGACCGGAACAAAAGTGAAGCTCTAAAGTACAAAATTGGGGCGATTGCATCCATCCTAGTCGCCGGTGCAGTCGGTGTTGGTATCCCAATTCTTGGGAAGACCATTCCGGCTTTACATCCCGATAGAAACATATTCTTCATCATCAAGGCCTTTGCAGCCGGGGTCATATTGGCTACTGGGTTCATCCACGTGCTCCCGGATGCGTTTGAGAGCTTGACGGACCCGGCTCTTAAGGAGAACCCATGGGGGAAATTTCCCTTCACTGGGTTTGTGGCCATGATGGCTGCCATTGGGACTTTGATGGTGGATTCTTATGCCACTTCTTATTACAGCAGGTCTCACTTCAAGAACAATATTAATGCAGACGAAGAGAAGGCCCAAGAGCATGAGGGTCACGTGCATGTTCATACTCATGCAACTCACGGCCATGCTCATGGAGCAGTTGACACTTCGCCCTCAGCAGAACTTCTTAGGCACAGAGTGATTTCACAGGTTTTGGAGTTGGGGATTGTTGTGCATTCTGTGATTATAGGAATTTCGTTAGGTGCATCTGAAAGTCCTAAAACAATAAAGCCTCTTGTAGCTGCCTTGTCTTTCCATCAATTTTTCGAAGGCATGGGACTCGGTGGATGCATAACTCAGGCGAAATTCAAGAATCGAGCTGTTGCAATCATGTCCTTGTTCTTCTCTCTTACAACTCCTGTTGGGATTGGCATCGGAATTGGAATATCAAATGTTTATGACGAAAGCAGCCCGACTGCACTCATCATGGAAGGCGTTTTTAACGCGGCATCAGCCGGTATCTTAATCTACATGTCCCTTGTTGATCTGCTAGCAGCTGATTTCATGAACCCAAAAATGCAGAGAAATGGAAGGCTTCAAGCTGGAGCTAACTTATCTCTTCTTCTCGGCGCTGGTTGCATGTCTTTGCTGGCCAAATGGGCTTAA

Protein Analysis

352

Amino Acids

37.46

Weight (kDa)

7.26

Isoelectric Point (pI)

34.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zip PF02535 45 - 348 1.9e-81 ZIP Zinc transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 21
Acc36I ACCTGC 1 cut(s) 432
AccB7I CCANNNNNTGG 1 cut(s) 200
AccII CGCG 1 cut(s) 896
AciI CCGC 1 cut(s) 896
AcoI YGGCCR 3 cut(s) 381, 532, 1041
AcsI RAATTY 3 cut(s) 359, 642, 755
AcvI CACGTG 2 cut(s) 298, 505
AdeI CACNNNGTG 1 cut(s) 589
AfaI GTAC 1 cut(s) 137
AfiI CCNNNNNNNGG 3 cut(s) 200, 371, 372
AflII CTTAAG 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 921
AgsI TTSAA 3 cut(s) 454, 760, 989
AluBI AGCT 7 cut(s) 128, 321, 692, 770, 947, 992, 998
AluI AGCT 7 cut(s) 128, 321, 692, 770, 947, 992, 998
Alw21I GWGCWC 2 cut(s) 83, 303
Alw26I GTCTC 1 cut(s) 450
Alw44I GTGCAC 1 cut(s) 79
AoxI GGCC 5 cut(s) 255, 381, 483, 532, 1041
ApaLI GTGCAC 1 cut(s) 79
ApeKI GCWGC 5 cut(s) 263, 392, 692, 858, 944
ApoI RAATTY 3 cut(s) 359, 642, 755
AseI ATTAAT 1 cut(s) 465
AspLEI GCGC 1 cut(s) 1022
AspS9I GGNCC 3 cut(s) 109, 328, 484
AsuC2I CCSGG 3 cut(s) 268, 305, 332
AsuII TTCGAA 1 cut(s) 717
AsuNHI GCTAGC 1 cut(s) 940
AvaII GGWCC 2 cut(s) 109, 328
BaeGI GKGCMC 1 cut(s) 83
BalI TGGCCA 2 cut(s) 383, 1043
BbrPI CACGTG 2 cut(s) 298, 505
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 83, 303
BbvCI CCTCAGC 1 cut(s) 565
BbvI GCAGC 5 cut(s) 275, 379, 679, 870, 956
BccI CCATC 5 cut(s) 98, 167, 382, 403, 714
BceAI ACGGC 1 cut(s) 547
BcgI CGANNNNNNTGC 2 cut(s) 712, 746
BciVI GTATCC 1 cut(s) 200
BcnI CCSGG 3 cut(s) 268, 305, 332
BcoDI GTCTC 1 cut(s) 450
BfaI CTAG 2 cut(s) 164, 941
BfoI RGCGCY 1 cut(s) 1023
BfrI CTTAAG 1 cut(s) 338
BfuAI ACCTGC 1 cut(s) 432
BfuI GTATCC 1 cut(s) 200
BglI GCCNNNNNGGC 2 cut(s) 389, 1049
BisI GCNGC 6 cut(s) 264, 393, 693, 859, 897, 945
BlsI GCNGC 6 cut(s) 265, 394, 694, 860, 898, 946
Bme1390I CCNGG 3 cut(s) 268, 305, 332
Bme18I GGWCC 2 cut(s) 109, 328
BmgT120I GGNCC 3 cut(s) 109, 328, 484
BmiI GGNNCC 2 cut(s) 110, 330
BmrFI CCNGG 3 cut(s) 268, 305, 332
BmrI ACTGGG 2 cut(s) 294, 381
BmsI GCATC 6 cut(s) 164, 298, 665, 728, 831, 908
BmtI GCTAGC 1 cut(s) 944
BmuI ACTGGG 2 cut(s) 294, 381
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 1 cut(s) 1014
Bpu10I CCTNAGC 1 cut(s) 565
Bpu14I TTCGAA 1 cut(s) 717
BpuMI CCSGG 3 cut(s) 268, 305, 332
BsaAI YACGTR 2 cut(s) 298, 505
BsaBI GATNNNNATC 1 cut(s) 821
BsaI GGTCTC 1 cut(s) 450
BsaJI CCNNGG 2 cut(s) 267, 350
BsaWI WCCGGW 1 cut(s) 111
Bsc4I CCNNNNNNNGG 3 cut(s) 200, 371, 372
Bse118I RCCGGY 2 cut(s) 170, 905
Bse1I ACTGG 2 cut(s) 289, 376
Bse8I GATNNNNATC 1 cut(s) 821
BseDI CCNNGG 2 cut(s) 267, 350
BseGI GGATG 7 cut(s) 109, 155, 159, 223, 291, 313, 743
BseJI GATNNNNATC 1 cut(s) 821
BseLI CCNNNNNNNGG 3 cut(s) 200, 371, 372
BseMII CTCAG 2 cut(s) 579, 761
BseNI ACTGG 2 cut(s) 289, 376
BseRI GAGGAG 1 cut(s) 35
BseSI GKGCMC 1 cut(s) 83
BseXI GCAGC 5 cut(s) 275, 379, 679, 870, 956
BsgI GTGCAG 2 cut(s) 195, 852
Bsh1236I CGCG 1 cut(s) 896
BshFI GGCC 5 cut(s) 257, 383, 485, 534, 1043
BsiHKAI GWGCWC 2 cut(s) 83, 303
BsiSI CCGG 7 cut(s) 112, 171, 215, 267, 305, 332, 906
BslFI GGGAC 4 cut(s) 122, 415, 741, 910
BslI CCNNNNNNNGG 3 cut(s) 200, 371, 372
BsmAI GTCTC 1 cut(s) 450
BsmFI GGGAC 4 cut(s) 122, 415, 741, 910
BsmI GAATGC 2 cut(s) 32, 625
BsnI GGCC 5 cut(s) 257, 383, 485, 534, 1043
Bso31I GGTCTC 1 cut(s) 450
Bsp119I TTCGAA 1 cut(s) 717
Bsp1286I GDGCHC 2 cut(s) 83, 303
Bsp143I GATC 1 cut(s) 934
Bsp19I CCATGG 1 cut(s) 350
BspACI CCGC 1 cut(s) 896
BspANI GGCC 5 cut(s) 257, 383, 485, 534, 1043
BspCNI CTCAG 2 cut(s) 578, 760
BspFNI CGCG 1 cut(s) 896
BspHI TCATGA 1 cut(s) 954
BspLI GGNNCC 2 cut(s) 110, 330
BspMI ACCTGC 1 cut(s) 432
BspOI GCTAGC 1 cut(s) 944
BspT104I TTCGAA 1 cut(s) 717
BspTI CTTAAG 1 cut(s) 338
BspTNI GGTCTC 1 cut(s) 450
BsrFI RCCGGY 2 cut(s) 170, 905
BsrI ACTGG 2 cut(s) 289, 376
BssAI RCCGGY 2 cut(s) 170, 905
BssECI CCNNGG 2 cut(s) 267, 350
BssMI GATC 1 cut(s) 934
BssT1I CCWWGG 1 cut(s) 350
Bst6I CTCTTC 3 cut(s) 53, 471, 1014
BstAFI CTTAAG 1 cut(s) 338
BstBAI YACGTR 2 cut(s) 298, 505
BstBI TTCGAA 1 cut(s) 717
BstC8I GCNNGC 2 cut(s) 942, 1041
BstDEI CTNAG 4 cut(s) 64, 565, 578, 747
BstDSI CCRYGG 1 cut(s) 350
BstF5I GGATG 7 cut(s) 109, 155, 159, 223, 291, 313, 743
BstFNI CGCG 1 cut(s) 896
BstH2I RGCGCY 1 cut(s) 1023
BstHHI GCGC 1 cut(s) 1022
BstKTI GATC 1 cut(s) 937
BstMAI GTCTC 1 cut(s) 450
BstMBI GATC 1 cut(s) 934
BstMWI GCNNNNNNNGC 6 cut(s) 263, 389, 545, 902, 1026, 1049
BstNSI RCATGY 3 cut(s) 512, 925, 1033
BstSCI CCNGG 3 cut(s) 266, 303, 330
BstSLI GKGCMC 1 cut(s) 83
BstUI CGCG 1 cut(s) 896
BstV1I GCAGC 5 cut(s) 275, 379, 679, 870, 956
BstV2I GAAGAC 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 542
BsuI GTATCC 1 cut(s) 200
BsuRI GGCC 5 cut(s) 257, 383, 485, 534, 1043
BtgI CCRYGG 1 cut(s) 350
BtsCI GGATG 7 cut(s) 109, 155, 159, 223, 291, 313, 743
BtsIMutI CAGTG 1 cut(s) 369
BveI ACCTGC 1 cut(s) 432
Cac8I GCNNGC 2 cut(s) 942, 1041
CciI TCATGA 1 cut(s) 954
CfoI GCGC 1 cut(s) 1022
Cfr10I RCCGGY 2 cut(s) 170, 905
Cfr13I GGNCC 3 cut(s) 109, 328, 484
Csp6I GTAC 1 cut(s) 136
CviQI GTAC 1 cut(s) 136
DdeI CTNAG 4 cut(s) 64, 565, 578, 747
DpnI GATC 1 cut(s) 936
DpnII GATC 1 cut(s) 934
DraIII CACNNNGTG 1 cut(s) 589
DrdI GACNNNNNNGTC 1 cut(s) 21
DseDI GACNNNNNNGTC 1 cut(s) 21
EaeI YGGCCR 3 cut(s) 381, 532, 1041
Eam1104I CTCTTC 3 cut(s) 53, 471, 1014
EarI CTCTTC 3 cut(s) 53, 471, 1014
Eco130I CCWWGG 1 cut(s) 350
Eco147I AGGCCT 1 cut(s) 257
Eco31I GGTCTC 1 cut(s) 450
Eco47I GGWCC 2 cut(s) 109, 328
Eco72I CACGTG 2 cut(s) 298, 505
EcoT14I CCWWGG 1 cut(s) 350
EcoT22I ATGCAT 1 cut(s) 743
ErhI CCWWGG 1 cut(s) 350
FaqI GGGAC 4 cut(s) 122, 415, 741, 910
Fnu4HI GCNGC 6 cut(s) 264, 393, 693, 859, 897, 945
FokI GGATG 7 cut(s) 116, 142, 146, 210, 278, 320, 750
Fsp4HI GCNGC 6 cut(s) 264, 393, 693, 859, 897, 945
FspBI CTAG 2 cut(s) 164, 941
GlaI GCGC 1 cut(s) 1021
GluI GCNGC 6 cut(s) 264, 393, 693, 859, 897, 945
GsuI CTGGAG 1 cut(s) 1014
HaeII RGCGCY 1 cut(s) 1023
HaeIII GGCC 5 cut(s) 257, 383, 485, 534, 1043
HapII CCGG 7 cut(s) 112, 171, 215, 267, 305, 332, 906
HhaI GCGC 1 cut(s) 1022
Hin6I GCGC 1 cut(s) 1020
HinP1I GCGC 1 cut(s) 1020
HincII GTYRAC 1 cut(s) 553
HindII GTYRAC 1 cut(s) 553
HinfI GANTC 3 cut(s) 416, 729, 763
HpaII CCGG 7 cut(s) 112, 171, 215, 267, 305, 332, 906
Hpy166II GTNNAC 2 cut(s) 81, 553
Hpy188I TCNGA 3 cut(s) 74, 661, 827
Hpy188III TCNNGA 4 cut(s) 227, 454, 760, 955
Hpy8I GTNNAC 2 cut(s) 81, 553
HpyAV CCTTC 6 cut(s) 92, 376, 475, 713, 875, 975
HpyCH4IV ACGT 2 cut(s) 297, 504
HpyF10VI GCNNNNNNNGC 6 cut(s) 263, 389, 545, 902, 1026, 1049
HpyF3I CTNAG 4 cut(s) 64, 565, 578, 747
HpySE526I ACGT 2 cut(s) 297, 504
HspAI GCGC 1 cut(s) 1020
Kzo9I GATC 1 cut(s) 934
LmnI GCTCC 3 cut(s) 306, 545, 995
Lsp1109I GCAGC 5 cut(s) 275, 379, 679, 870, 956
LweI GCATC 6 cut(s) 164, 298, 665, 728, 831, 908
MaeI CTAG 2 cut(s) 164, 941
MaeII ACGT 2 cut(s) 297, 504
MaeIII GTNAC 1 cut(s) 500
MalI GATC 1 cut(s) 936
MboI GATC 1 cut(s) 934
MboII GAAGA 7 cut(s) 40, 217, 235, 488, 784, 1001, 1004
MhlI GDGCHC 2 cut(s) 83, 303
MlsI TGGCCA 2 cut(s) 383, 1043
MluCI AATT 7 cut(s) 141, 195, 359, 642, 710, 755, 828
MluNI TGGCCA 2 cut(s) 383, 1043
MlyI GAGTC 1 cut(s) 723
MnlI CCTC 5 cut(s) 53, 56, 490, 574, 693
Mox20I TGGCCA 2 cut(s) 383, 1043
Mph1103I ATGCAT 1 cut(s) 743
MscI TGGCCA 2 cut(s) 383, 1043
MseI TTAA 5 cut(s) 339, 465, 891, 914, 1054
MslI CAYNNNNRTG 5 cut(s) 296, 507, 519, 534, 540
Msp20I TGGCCA 2 cut(s) 383, 1043
MspA1I CMGCKG 1 cut(s) 947
MspCI CTTAAG 1 cut(s) 338
MspI CCGG 7 cut(s) 112, 171, 215, 267, 305, 332, 906
MspR9I CCNGG 3 cut(s) 268, 305, 332
Mva1269I GAATGC 2 cut(s) 32, 625
MvnI CGCG 1 cut(s) 896
MwoI GCNNNNNNNGC 6 cut(s) 263, 389, 545, 902, 1026, 1049
NciI CCSGG 3 cut(s) 268, 305, 332
NcoI CCATGG 1 cut(s) 350
NdeII GATC 1 cut(s) 934
NheI GCTAGC 1 cut(s) 940
NlaIV GGNNCC 2 cut(s) 110, 330
NmeAIII GCCGAG 1 cut(s) 996
NmuCI GTSAC 1 cut(s) 500
NsiI ATGCAT 1 cut(s) 743
NspI RCATGY 3 cut(s) 512, 925, 1033
NspV TTCGAA 1 cut(s) 717
PagI TCATGA 1 cut(s) 954
PceI AGGCCT 1 cut(s) 257
PciI ACATGT 1 cut(s) 921
PctI GAATGC 2 cut(s) 32, 625
PfeI GAWTC 2 cut(s) 416, 763
PflMI CCANNNNNTGG 1 cut(s) 200
PfoI TCCNGGA 1 cut(s) 303
PkrI GCNGC 6 cut(s) 265, 394, 694, 860, 898, 946
PleI GAGTC 1 cut(s) 723
PmaCI CACGTG 2 cut(s) 298, 505
PmlI CACGTG 2 cut(s) 298, 505
PpsI GAGTC 1 cut(s) 723
Ppu21I YACGTR 2 cut(s) 298, 505
PscI ACATGT 1 cut(s) 921
PshBI ATTAAT 1 cut(s) 465
PspCI CACGTG 2 cut(s) 298, 505
PspN4I GGNNCC 2 cut(s) 110, 330
PspPI GGNCC 3 cut(s) 109, 328, 484
PvuII CAGCTG 1 cut(s) 947
RsaI GTAC 1 cut(s) 137
RsaNI GTAC 1 cut(s) 136
RseI CAYNNNNRTG 5 cut(s) 296, 507, 519, 534, 540
SaqAI TTAA 5 cut(s) 339, 465, 891, 914, 1054
SatI GCNGC 6 cut(s) 264, 393, 693, 859, 897, 945
Sau3AI GATC 1 cut(s) 934
Sau96I GGNCC 3 cut(s) 109, 328, 484
SchI GAGTC 1 cut(s) 723
ScrFI CCNGG 3 cut(s) 268, 305, 332
SduI GDGCHC 2 cut(s) 83, 303
SfaNI GCATC 6 cut(s) 164, 298, 665, 728, 831, 908
SfuI TTCGAA 1 cut(s) 717
SgrAI CRCCGGYG 1 cut(s) 170
SinI GGWCC 2 cut(s) 109, 328
SmiMI CAYNNNNRTG 5 cut(s) 296, 507, 519, 534, 540
SmlI CTYRAG 1 cut(s) 338
SmoI CTYRAG 1 cut(s) 338
Sse9I AATT 7 cut(s) 141, 195, 359, 642, 710, 755, 828
SseBI AGGCCT 1 cut(s) 257
SsiI CCGC 1 cut(s) 896
SspI AATATT 1 cut(s) 463
SspMI CTAG 2 cut(s) 164, 941
StuI AGGCCT 1 cut(s) 257
StyD4I CCNGG 3 cut(s) 266, 303, 330
StyI CCWWGG 1 cut(s) 350
TaiI ACGT 2 cut(s) 300, 507
TaqI TCGA 3 cut(s) 13, 717, 766
TasI AATT 7 cut(s) 141, 195, 359, 642, 710, 755, 828
TatI WGTACW 1 cut(s) 135
TauI GCSGC 1 cut(s) 899
TfiI GAWTC 2 cut(s) 416, 763
Tru1I TTAA 5 cut(s) 339, 465, 891, 914, 1054
Tru9I TTAA 5 cut(s) 339, 465, 891, 914, 1054
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 5 cut(s) 263, 392, 692, 858, 944
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 6 cut(s) 40, 235, 280, 503, 943, 971
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 1 cut(s) 376
Van91I CCANNNNNTGG 1 cut(s) 200
Vha464I CTTAAG 1 cut(s) 338
VneI GTGCAC 1 cut(s) 79
VpaK11BI GGWCC 2 cut(s) 109, 328
VspI ATTAAT 1 cut(s) 465
XapI RAATTY 3 cut(s) 359, 642, 755
XceI RCATGY 3 cut(s) 512, 925, 1033
XspI CTAG 2 cut(s) 164, 941
Zsp2I ATGCAT 1 cut(s) 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.