AT1G07950

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. The Mediator complex, having a compact conformation in its free form, is recruited to promoters by direct interactions with regulatory proteins and serves for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012927
Physical Location & Seq
Reverse (-)
2465729 .. 2467165
1437 bp
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UTR
Exon/CDS
Intron
AT1G07950.2

Sequence Viewer

Length: 465 bp
ATGAACAAAGGCGGTGGATCCGGTGGCGGATCCGGTGGAGGAAGCGGTCCAACGGCCGCCGCCGCCGCCGCGGCTTTACAGAAGCAAAAGGCGTTGTTACAGAGAGTGGAGACTGACATCACATCTGTCGTCGATAACTTCACGCAGATCGTCAATGTTTCAAGGGTGAGTGATCCGCCGGTGAAGAACTCGCAGGAGACATACATGATGGAGATGCGAGCTTCTAGAATGGTTCAAGCAGCTGATTCTCTTTTGAAACTCGTATCAGAACTTAAGCAAACAGCGATATTTTCAGGATTTGCATCACTAAACGATCATGTAGAACAAAGAATAGAAGAGTTTGATCAAGAGGCCGAGAAGACAAATCGATTGTTGGCTAGAATTGCTGATGATGCATCTGCCAATCTTAAAGAGCTCGAGTCTCACTATTACTCTTCTGCTCAGAGATTGACTCTAGACATCTGA

Protein Analysis

154

Amino Acids

16.56

Weight (kDa)

5.2

Isoelectric Point (pI)

42.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med22 PF06179 64 - 163 2.8e-30 Surfeit locus protein 5 subunit 22 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07950 AT1G07950 AT1G16430
fragaria_vesca FvH4_3g13800
malus_domestica MD02G1009700.v1.1
prunus_persica Prupe.7G260300_v2.0.a1 Prupe.7G260300_v2.0.a1
pyrus_communis pycom15g13880
rosa_chinensis RchiOBHm_Chr5g0022991
rosa_laevigata RLG00000032687
rosa_multiflora Rmu_sc0002675.1_g000006
rosa_roxburghii Rroxscaffold_1G00056350
rosa_rugosa Rorug05G0076300
rosa_samantha Rh5AG162500 Rh5BG161700 Rh5CG176400 Rh5DG163100
rosa_wichuraiana Rw5G014640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 71
AclWI GGATC 5 cut(s) 12, 24, 25, 37, 167
AcoI YGGCCR 1 cut(s) 54
AflII CTTAAG 1 cut(s) 272
AgsI TTSAA 3 cut(s) 162, 236, 256
AluBI AGCT 3 cut(s) 221, 242, 415
AluI AGCT 3 cut(s) 221, 242, 415
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 3 cut(s) 104, 191, 426
AlwI GGATC 5 cut(s) 12, 24, 25, 37, 167
Ama87I CYCGRG 1 cut(s) 416
AoxI GGCC 2 cut(s) 54, 351
ApeKI GCWGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 47
AsuHPI GGTGA 2 cut(s) 178, 193
AvaI CYCGRG 1 cut(s) 416
AvaII GGWCC 1 cut(s) 47
BamHI GGATCC 2 cut(s) 17, 29
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 365
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 1 cut(s) 251
BccI CCATC 1 cut(s) 202
BceAI ACGGC 1 cut(s) 69
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 3 cut(s) 104, 191, 426
BfaI CTAG 3 cut(s) 225, 378, 455
BfrI CTTAAG 1 cut(s) 272
BglI GCCNNNNNGGC 1 cut(s) 71
BisI GCNGC 7 cut(s) 57, 60, 63, 66, 69, 72, 240
BlsI GCNGC 7 cut(s) 58, 61, 64, 67, 70, 73, 241
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 416
BmgT120I GGNCC 1 cut(s) 47
BmiI GGNNCC 2 cut(s) 19, 31
BmsI GCATC 4 cut(s) 204, 311, 382, 404
BpiI GAAGAC 1 cut(s) 365
BplI GAGNNNNNCTC 1 cut(s) 436
Bsa29I ATCGAT 1 cut(s) 367
BsaBI GATNNNNATC 1 cut(s) 301
BsaJI CCNNGG 1 cut(s) 69
BsaWI WCCGGW 2 cut(s) 20, 32
Bse118I RCCGGY 1 cut(s) 178
Bse8I GATNNNNATC 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 367
BseDI CCNNGG 1 cut(s) 69
BseJI GATNNNNATC 1 cut(s) 301
BseMII CTCAG 1 cut(s) 455
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 1 cut(s) 251
Bsh1236I CGCG 1 cut(s) 71
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 2 cut(s) 56, 353
BshVI ATCGAT 1 cut(s) 367
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 417
BsiHKCI CYCGRG 1 cut(s) 416
BsiSI CCGG 3 cut(s) 21, 33, 179
BsmAI GTCTC 3 cut(s) 104, 191, 426
BsnI GGCC 2 cut(s) 56, 353
BsoBI CYCGRG 1 cut(s) 416
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 6 cut(s) 17, 29, 147, 172, 313, 343
BspANI GGCC 2 cut(s) 56, 353
BspCNI CTCAG 1 cut(s) 454
BspDI ATCGAT 1 cut(s) 367
BspFNI CGCG 1 cut(s) 71
BspLI GGNNCC 2 cut(s) 19, 31
BspPI GGATC 5 cut(s) 12, 24, 25, 37, 167
BspTI CTTAAG 1 cut(s) 272
BsrFI RCCGGY 1 cut(s) 178
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 69
BssMI GATC 6 cut(s) 17, 29, 147, 172, 313, 343
Bst6I CTCTTC 2 cut(s) 330, 439
BstAFI CTTAAG 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 219
BstDEI CTNAG 1 cut(s) 441
BstDSI CCRYGG 1 cut(s) 69
BstFNI CGCG 1 cut(s) 71
BstKTI GATC 6 cut(s) 20, 32, 150, 175, 316, 346
BstMAI GTCTC 3 cut(s) 104, 191, 426
BstMBI GATC 6 cut(s) 17, 29, 147, 172, 313, 343
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 6 cut(s) 62, 65, 68, 71, 383, 392
BstUI CGCG 1 cut(s) 71
BstV1I GCAGC 1 cut(s) 251
BstV2I GAAGAC 1 cut(s) 365
BstX2I RGATCY 2 cut(s) 17, 29
BstYI RGATCY 2 cut(s) 17, 29
BstZI CGGCCG 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 367
BsuRI GGCC 2 cut(s) 56, 353
BsuTUI ATCGAT 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 69
Cac8I GCNNGC 1 cut(s) 219
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 367
CspCI CAANNNNNGTGG 1 cut(s) 30
CviAII CATG 2 cut(s) 205, 317
CviJI RGCY 7 cut(s) 56, 74, 221, 242, 353, 377, 415
CviKI_1 RGCY 7 cut(s) 56, 74, 221, 242, 353, 377, 415
DdeI CTNAG 1 cut(s) 441
DpnI GATC 6 cut(s) 19, 31, 149, 174, 315, 345
DpnII GATC 6 cut(s) 17, 29, 147, 172, 313, 343
EaeI YGGCCR 1 cut(s) 54
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 330, 439
EarI CTCTTC 2 cut(s) 330, 439
EciI GGCGGA 2 cut(s) 42, 165
Ecl136II GAGCTC 1 cut(s) 415
EclXI CGGCCG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 417
Eco47I GGWCC 1 cut(s) 47
Eco52I CGGCCG 1 cut(s) 54
Eco53kI GAGCTC 1 cut(s) 415
Eco88I CYCGRG 1 cut(s) 416
EcoICRI GAGCTC 1 cut(s) 415
EcoT22I ATGCAT 1 cut(s) 397
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 2 cut(s) 208, 320
FaiI YATR 3 cut(s) 202, 206, 318
FatI CATG 2 cut(s) 204, 316
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 7 cut(s) 57, 60, 63, 66, 69, 72, 240
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 7 cut(s) 57, 60, 63, 66, 69, 72, 240
FspBI CTAG 3 cut(s) 225, 378, 455
GluI GCNGC 7 cut(s) 57, 60, 63, 66, 69, 72, 240
HaeIII GGCC 2 cut(s) 56, 353
HapII CCGG 3 cut(s) 21, 33, 179
Hin1II CATG 2 cut(s) 208, 320
HinfI GANTC 3 cut(s) 245, 419, 451
HpaII CCGG 3 cut(s) 21, 33, 179
HphI GGTGA 2 cut(s) 178, 193
Hpy188I TCNGA 3 cut(s) 268, 444, 464
Hpy188III TCNNGA 4 cut(s) 225, 294, 347, 455
Hpy99I CGWCG 1 cut(s) 134
HpyCH4V TGCA 2 cut(s) 302, 395
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 65, 68, 71, 383, 392
HpyF3I CTNAG 1 cut(s) 441
Hsp92II CATG 2 cut(s) 208, 320
Ksp22I TGATCA 1 cut(s) 343
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 6 cut(s) 17, 29, 147, 172, 313, 343
LpnPI CCDG 5 cut(s) 34, 46, 179, 192, 279
Lsp1109I GCAGC 1 cut(s) 251
LweI GCATC 4 cut(s) 204, 311, 382, 404
MaeI CTAG 3 cut(s) 225, 378, 455
MaeIII GTNAC 1 cut(s) 96
MalI GATC 6 cut(s) 19, 31, 149, 174, 315, 345
MboI GATC 6 cut(s) 17, 29, 147, 172, 313, 343
MboII GAAGA 4 cut(s) 196, 347, 370, 426
MflI RGATCY 2 cut(s) 17, 29
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 1 cut(s) 381
MlyI GAGTC 2 cut(s) 428, 445
MmeI TCCRAC 1 cut(s) 74
MnlI CCTC 2 cut(s) 32, 343
Mph1103I ATGCAT 1 cut(s) 397
MseI TTAA 2 cut(s) 273, 408
MspA1I CMGCKG 2 cut(s) 71, 242
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 3 cut(s) 21, 33, 179
MvnI CGCG 1 cut(s) 71
MwoI GCNNNNNNNGC 6 cut(s) 62, 65, 68, 71, 383, 392
NdeII GATC 6 cut(s) 17, 29, 147, 172, 313, 343
NlaIII CATG 2 cut(s) 208, 320
NlaIV GGNNCC 2 cut(s) 19, 31
NmeAIII GCCGAG 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 397
PaeR7I CTCGAG 1 cut(s) 416
PfeI GAWTC 1 cut(s) 245
PkrI GCNGC 7 cut(s) 58, 61, 64, 67, 70, 73, 241
PleI GAGTC 2 cut(s) 427, 445
PpsI GAGTC 2 cut(s) 427, 445
Psp124BI GAGCTC 1 cut(s) 417
PspN4I GGNNCC 2 cut(s) 19, 31
PspPI GGNCC 1 cut(s) 47
PspXI VCTCGAGB 1 cut(s) 416
PsuI RGATCY 2 cut(s) 17, 29
PvuII CAGCTG 1 cut(s) 242
SacI GAGCTC 1 cut(s) 417
SacII CCGCGG 1 cut(s) 72
SaqAI TTAA 2 cut(s) 273, 408
SatI GCNGC 7 cut(s) 57, 60, 63, 66, 69, 72, 240
Sau3AI GATC 6 cut(s) 17, 29, 147, 172, 313, 343
Sau96I GGNCC 1 cut(s) 47
SchI GAGTC 2 cut(s) 428, 445
SduI GDGCHC 1 cut(s) 417
SetI ASST 3 cut(s) 223, 244, 417
SfaNI GCATC 4 cut(s) 204, 311, 382, 404
Sfr274I CTCGAG 1 cut(s) 416
Sfr303I CCGCGG 1 cut(s) 72
SgrAI CRCCGGYG 1 cut(s) 178
SgrBI CCGCGG 1 cut(s) 72
SinI GGWCC 1 cut(s) 47
SlaI CTCGAG 1 cut(s) 416
SmlI CTYRAG 2 cut(s) 272, 416
SmoI CTYRAG 2 cut(s) 272, 416
Sse9I AATT 1 cut(s) 381
SspMI CTAG 3 cut(s) 225, 378, 455
SstI GAGCTC 1 cut(s) 417
TaqI TCGA 3 cut(s) 132, 367, 417
TasI AATT 1 cut(s) 381
TauI GCSGC 6 cut(s) 59, 62, 65, 68, 71, 74
TfiI GAWTC 1 cut(s) 245
Tru1I TTAA 2 cut(s) 273, 408
Tru9I TTAA 2 cut(s) 273, 408
TseI GCWGC 1 cut(s) 239
TspDTI ATGAA 1 cut(s) 17
Vha464I CTTAAG 1 cut(s) 272
VpaK11BI GGWCC 1 cut(s) 47
XbaI TCTAGA 2 cut(s) 224, 454
XhoI CTCGAG 1 cut(s) 416
XspI CTAG 3 cut(s) 225, 378, 455
Zsp2I ATGCAT 1 cut(s) 397
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.