Rh5AG162500

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0012927
Physical Location & Seq
Reverse (-)
17420761 .. 17422291
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG162500.1

Sequence Viewer

Length: 492 bp
ATGAACAGAGGGGGAGGAGGAGGCGGAGCAGGAGCCGGAGGCGGGTCCGGCGCCGGGAGTGGGCCCACATCGGCGGCGGCGTTGGCGGCAGCTAAGAAGCAGAAGAAGTGGCTGGAGAGTGTGGAAACAGACATCGCCAACATCGTCGACCATTTCAGCTACCTCGTCAACGTATCCAGGGTGAATGAGCCACCTGTTAGGAACTCTCAAGAAGCTTTCATGATGGAGATGCGTGCTGCTAGAATGGTTCAGGCATCTGATTCGCTACTTAAGTTGGTGTCAGACCTGAAGCAGACTGCACTCTTTTCTGGATTTGCATCCCTTAATGACCATGTAGAGGAAAGAATGGCAGAGTTTGACAAGCAGGCTGAGAAGACTGACCGCATGTTAGCTAGAATTGGGGAGGAGGCAGCTGCCAGCCTCAAGGAGCTTGAAGCCCATTATTATTCTTCTTCGGTACCACATACACATGAAAACTACTTGCAGCAATGA

Protein Analysis

163

Amino Acids

17.49

Weight (kDa)

5.73

Isoelectric Point (pI)

39.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med22 PF06179 40 - 138 4e-28 Surfeit locus protein 5 subunit 22 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07950 AT1G07950 AT1G16430
fragaria_vesca FvH4_3g13800
malus_domestica MD02G1009700.v1.1
prunus_persica Prupe.7G260300_v2.0.a1 Prupe.7G260300_v2.0.a1
pyrus_communis pycom15g13880
rosa_chinensis RchiOBHm_Chr5g0022991
rosa_laevigata RLG00000032687
rosa_multiflora Rmu_sc0002675.1_g000006
rosa_roxburghii Rroxscaffold_1G00056350
rosa_rugosa Rorug05G0076300
rosa_samantha Rh5AG162500 Rh5BG161700 Rh5CG176400 Rh5DG163100
rosa_wichuraiana Rw5G014640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 457
AccB1I GGYRCC 2 cut(s) 50, 457
AccI GTMKAC 1 cut(s) 147
AciI CCGC 6 cut(s) 24, 42, 74, 77, 86, 382
AcuI CTGAAG 1 cut(s) 308
AcyI GRCGYC 1 cut(s) 51
AfaI GTAC 1 cut(s) 459
AfiI CCNNNNNNNGG 4 cut(s) 42, 54, 60, 337
AflII CTTAAG 1 cut(s) 269
AgsI TTSAA 1 cut(s) 434
AjnI CCWGG 1 cut(s) 176
AluBI AGCT 6 cut(s) 92, 159, 215, 392, 413, 430
AluI AGCT 6 cut(s) 92, 159, 215, 392, 413, 430
AoxI GGCC 1 cut(s) 62
ApaI GGGCCC 1 cut(s) 66
ApeKI GCWGC 5 cut(s) 89, 236, 410, 413, 484
Asp718I GGTACC 1 cut(s) 457
AspLEI GCGC 1 cut(s) 53
AspS9I GGNCC 3 cut(s) 45, 62, 63
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 1 cut(s) 193
AvaII GGWCC 1 cut(s) 45
BaeGI GKGCMC 1 cut(s) 66
BanI GGYRCC 2 cut(s) 50, 457
BanII GRGCYC 1 cut(s) 66
BbsI GAAGAC 1 cut(s) 380
BbvI GCAGC 4 cut(s) 101, 223, 400, 422
BccI CCATC 1 cut(s) 217
BcgI CGANNNNNNTGC 2 cut(s) 243, 277
BciT130I CCWGG 1 cut(s) 178
BciVI GTATCC 1 cut(s) 184
BcnI CCSGG 1 cut(s) 55
BfaI CTAG 2 cut(s) 240, 393
BfoI RGCGCY 1 cut(s) 54
BfrI CTTAAG 1 cut(s) 269
BfuI GTATCC 1 cut(s) 184
BisI GCNGC 8 cut(s) 75, 78, 87, 90, 237, 411, 414, 485
BlsI GCNGC 8 cut(s) 76, 79, 88, 91, 238, 412, 415, 486
Bme1390I CCNGG 2 cut(s) 55, 178
Bme18I GGWCC 1 cut(s) 45
BmgT120I GGNCC 3 cut(s) 45, 62, 63
BmiI GGNNCC 5 cut(s) 34, 46, 52, 64, 459
BmrFI CCNGG 2 cut(s) 55, 178
BmsI GCATC 3 cut(s) 219, 263, 326
BpiI GAAGAC 1 cut(s) 380
BpmI CTGGAG 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 192, 407
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 316
BsaHI GRCGYC 1 cut(s) 51
BsaJI CCNNGG 1 cut(s) 177
Bsc4I CCNNNNNNNGG 4 cut(s) 42, 54, 60, 337
Bse8I GATNNNNATC 1 cut(s) 316
BseBI CCWGG 1 cut(s) 178
BseDI CCNNGG 1 cut(s) 177
BseGI GGATG 1 cut(s) 317
BseJI GATNNNNATC 1 cut(s) 316
BseLI CCNNNNNNNGG 4 cut(s) 42, 54, 60, 337
BseMII CTCAG 1 cut(s) 360
BseRI GAGGAG 3 cut(s) 30, 33, 419
BseSI GKGCMC 1 cut(s) 66
BseXI GCAGC 4 cut(s) 101, 223, 400, 422
BsgI GTGCAG 1 cut(s) 282
BshFI GGCC 1 cut(s) 64
BshNI GGYRCC 2 cut(s) 50, 457
BsiSI CCGG 3 cut(s) 36, 48, 54
BslI CCNNNNNNNGG 4 cut(s) 42, 54, 60, 337
BsnI GGCC 1 cut(s) 64
Bsp120I GGGCCC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 66
BspACI CCGC 6 cut(s) 24, 42, 74, 77, 86, 382
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 361
BspHI TCATGA 1 cut(s) 219
BspLI GGNNCC 5 cut(s) 34, 46, 52, 64, 459
BspT107I GGYRCC 2 cut(s) 50, 457
BspTI CTTAAG 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 177
BssNI GRCGYC 1 cut(s) 51
Bst2UI CCWGG 1 cut(s) 178
BstACI GRCGYC 1 cut(s) 51
BstAFI CTTAAG 1 cut(s) 269
BstC8I GCNNGC 3 cut(s) 234, 366, 418
BstDEI CTNAG 2 cut(s) 93, 369
BstF5I GGATG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 54
BstHHI GCGC 1 cut(s) 53
BstMWI GCNNNNNNNGC 3 cut(s) 48, 83, 86
BstNI CCWGG 1 cut(s) 178
BstNSI RCATGY 1 cut(s) 388
BstSCI CCNGG 2 cut(s) 53, 176
BstSLI GKGCMC 1 cut(s) 66
BstV1I GCAGC 4 cut(s) 101, 223, 400, 422
BstV2I GAAGAC 1 cut(s) 380
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 1 cut(s) 64
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 1 cut(s) 317
Cac8I GCNNGC 3 cut(s) 234, 366, 418
CciI TCATGA 1 cut(s) 219
CfoI GCGC 1 cut(s) 53
Cfr13I GGNCC 3 cut(s) 45, 62, 63
Csp6I GTAC 1 cut(s) 458
CviAII CATG 4 cut(s) 220, 332, 385, 470
CviQI GTAC 1 cut(s) 458
DdeI CTNAG 2 cut(s) 93, 369
DinI GGCGCC 1 cut(s) 52
EciI GGCGGA 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 45
Eco57I CTGAAG 1 cut(s) 308
EcoRII CCWGG 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 66
EgeI GGCGCC 1 cut(s) 52
EheI GGCGCC 1 cut(s) 52
FaeI CATG 4 cut(s) 223, 335, 388, 473
FaiI YATR 5 cut(s) 221, 333, 386, 465, 471
FatI CATG 4 cut(s) 219, 331, 384, 469
FauI CCCGC 1 cut(s) 35
FblI GTMKAC 1 cut(s) 147
Fnu4HI GCNGC 8 cut(s) 75, 78, 87, 90, 237, 411, 414, 485
FokI GGATG 1 cut(s) 304
FriOI GRGCYC 1 cut(s) 66
Fsp4HI GCNGC 8 cut(s) 75, 78, 87, 90, 237, 411, 414, 485
FspBI CTAG 2 cut(s) 240, 393
GlaI GCGC 1 cut(s) 52
GluI GCNGC 8 cut(s) 75, 78, 87, 90, 237, 411, 414, 485
GsuI CTGGAG 1 cut(s) 134
HaeII RGCGCY 1 cut(s) 54
HaeIII GGCC 1 cut(s) 64
HapII CCGG 3 cut(s) 36, 48, 54
HhaI GCGC 1 cut(s) 53
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 4 cut(s) 223, 335, 388, 473
Hin6I GCGC 1 cut(s) 51
HinP1I GCGC 1 cut(s) 51
HincII GTYRAC 2 cut(s) 148, 169
HindII GTYRAC 2 cut(s) 148, 169
HindIII AAGCTT 1 cut(s) 213
HinfI GANTC 1 cut(s) 260
HpaII CCGG 3 cut(s) 36, 48, 54
HphI GGTGA 1 cut(s) 193
Hpy166II GTNNAC 2 cut(s) 148, 169
Hpy188I TCNGA 2 cut(s) 259, 283
Hpy188III TCNNGA 3 cut(s) 209, 220, 309
Hpy8I GTNNAC 2 cut(s) 148, 169
Hpy99I CGWCG 1 cut(s) 149
HpyCH4IV ACGT 1 cut(s) 171
HpyCH4V TGCA 3 cut(s) 299, 317, 484
HpyF10VI GCNNNNNNNGC 3 cut(s) 48, 83, 86
HpyF3I CTNAG 2 cut(s) 93, 369
HpySE526I ACGT 1 cut(s) 171
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 4 cut(s) 223, 335, 388, 473
HspAI GCGC 1 cut(s) 51
KasI GGCGCC 1 cut(s) 50
KpnI GGTACC 1 cut(s) 461
LmnI GCTCC 3 cut(s) 26, 32, 427
Lsp1109I GCAGC 4 cut(s) 101, 223, 400, 422
LweI GCATC 3 cut(s) 219, 263, 326
MaeI CTAG 2 cut(s) 240, 393
MaeII ACGT 1 cut(s) 171
MboII GAAGA 4 cut(s) 115, 385, 441, 444
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 1 cut(s) 396
Mly113I GGCGCC 1 cut(s) 51
MnlI CCTC 9 cut(s) 8, 11, 14, 32, 173, 331, 397, 400, 431
MseI TTAA 2 cut(s) 270, 324
MslI CAYNNNNRTG 1 cut(s) 468
MspA1I CMGCKG 1 cut(s) 413
MspCI CTTAAG 1 cut(s) 269
MspI CCGG 3 cut(s) 36, 48, 54
MspR9I CCNGG 2 cut(s) 55, 178
MvaI CCWGG 1 cut(s) 178
MwoI GCNNNNNNNGC 3 cut(s) 48, 83, 86
NarI GGCGCC 1 cut(s) 51
NciI CCSGG 1 cut(s) 55
NlaIII CATG 4 cut(s) 223, 335, 388, 473
NlaIV GGNNCC 5 cut(s) 34, 46, 52, 64, 459
NspI RCATGY 1 cut(s) 388
PagI TCATGA 1 cut(s) 219
PcsI WCGNNNNNNNCGW 2 cut(s) 77, 141
PfeI GAWTC 1 cut(s) 260
PkrI GCNGC 8 cut(s) 76, 79, 88, 91, 238, 412, 415, 486
PluTI GGCGCC 1 cut(s) 54
Psp6I CCWGG 1 cut(s) 176
PspGI CCWGG 1 cut(s) 176
PspN4I GGNNCC 5 cut(s) 34, 46, 52, 64, 459
PspOMI GGGCCC 1 cut(s) 62
PspPI GGNCC 3 cut(s) 45, 62, 63
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 1 cut(s) 459
RsaNI GTAC 1 cut(s) 458
RseI CAYNNNNRTG 1 cut(s) 468
SalI GTCGAC 1 cut(s) 146
SaqAI TTAA 2 cut(s) 270, 324
SatI GCNGC 8 cut(s) 75, 78, 87, 90, 237, 411, 414, 485
Sau96I GGNCC 3 cut(s) 45, 62, 63
ScrFI CCNGG 2 cut(s) 55, 178
SduI GDGCHC 1 cut(s) 66
SfaNI GCATC 3 cut(s) 219, 263, 326
SfoI GGCGCC 1 cut(s) 52
SinI GGWCC 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 468
SmlI CTYRAG 3 cut(s) 207, 269, 422
SmoI CTYRAG 3 cut(s) 207, 269, 422
Sse9I AATT 1 cut(s) 396
SsiI CCGC 6 cut(s) 24, 42, 74, 77, 86, 382
SspDI GGCGCC 1 cut(s) 50
SspMI CTAG 2 cut(s) 240, 393
StyD4I CCNGG 2 cut(s) 53, 176
TaiI ACGT 1 cut(s) 174
TaqI TCGA 1 cut(s) 147
TasI AATT 1 cut(s) 396
TauI GCSGC 3 cut(s) 77, 80, 89
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 2 cut(s) 270, 324
Tru9I TTAA 2 cut(s) 270, 324
TseI GCWGC 5 cut(s) 89, 236, 410, 413, 484
TspDTI ATGAA 3 cut(s) 17, 208, 486
Vha464I CTTAAG 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 45
XceI RCATGY 1 cut(s) 388
XmiI GTMKAC 1 cut(s) 147
XspI CTAG 2 cut(s) 240, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.