RLG00000032687

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0012927
Physical Location & Seq
Reverse (-)
17819903 .. 17821095
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032687

Sequence Viewer

Length: 498 bp
ATGAACAGAGGGGGAGGAGGAGGCGGAGCAGGAGCCGGAGCCGGAGGTGGGTCCGGGGCCGGGAGTGGGCCCACATCGGCGGCGGCGTTGGCGGCAGCTAAGAAGCAGAAGAAGTGGCTGGAGAGTGTGGAAACAGACATCGCCAACATCGTCGACCATTTCAACAACCTCGTCAACGTATCCAGGGTGAATGAGCCACCTGTTAGGAACTCTCAAGAAGCTTTCATGATGGAGATGCGTGCTGCTAGAATGGTTCAGGCATCTGATTCTCTACTTAAGTTGGTGTCCGACCTGAAGCAGACTGCACTCTTTTCTGGATTTGCATCCCTTAATGACCATGTAGAGGAAAGAATGGCAGAGTTTGACAAGCAGGCCGAGAAGACTGACCGCATGTTAGCTAGAATTGGAGAGGAGGCAGCTGCCAGCCTCAAGGAGCTTGAAGCCCACTATTATTCTTCTTCGGTACCACATACACATGAAAACTACTTGCAGCAATGA

Protein Analysis

166

Amino Acids

17.59

Weight (kDa)

5.73

Isoelectric Point (pI)

38.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med22 PF06179 42 - 140 8.9e-29 Surfeit locus protein 5 subunit 22 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0012927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07950 AT1G07950 AT1G16430
fragaria_vesca FvH4_3g13800
malus_domestica MD02G1009700.v1.1
prunus_persica Prupe.7G260300_v2.0.a1 Prupe.7G260300_v2.0.a1
pyrus_communis pycom15g13880
rosa_chinensis RchiOBHm_Chr5g0022991
rosa_laevigata RLG00000032687
rosa_multiflora Rmu_sc0002675.1_g000006
rosa_roxburghii Rroxscaffold_1G00056350
rosa_rugosa Rorug05G0076300
rosa_samantha Rh5AG162500 Rh5BG161700 Rh5CG176400 Rh5DG163100
rosa_wichuraiana Rw5G014640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 463
AccB1I GGYRCC 1 cut(s) 463
AccI GTMKAC 1 cut(s) 153
AciI CCGC 5 cut(s) 24, 80, 83, 92, 388
AcuI CTGAAG 1 cut(s) 314
AfaI GTAC 1 cut(s) 465
AfiI CCNNNNNNNGG 4 cut(s) 48, 60, 66, 343
AflII CTTAAG 1 cut(s) 275
AgsI TTSAA 2 cut(s) 163, 440
AjnI CCWGG 1 cut(s) 182
AluBI AGCT 5 cut(s) 98, 221, 398, 419, 436
AluI AGCT 5 cut(s) 98, 221, 398, 419, 436
AoxI GGCC 3 cut(s) 57, 68, 372
ApaI GGGCCC 1 cut(s) 72
ApeKI GCWGC 5 cut(s) 95, 242, 416, 419, 490
Asp718I GGTACC 1 cut(s) 463
AspS9I GGNCC 4 cut(s) 51, 57, 68, 69
AsuC2I CCSGG 2 cut(s) 55, 61
AsuHPI GGTGA 1 cut(s) 199
AvaII GGWCC 1 cut(s) 51
BaeGI GKGCMC 1 cut(s) 72
BanI GGYRCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 72
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 4 cut(s) 107, 229, 406, 428
BccI CCATC 1 cut(s) 223
BciT130I CCWGG 1 cut(s) 184
BciVI GTATCC 1 cut(s) 190
BcnI CCSGG 2 cut(s) 55, 61
BfaI CTAG 2 cut(s) 246, 399
BfrI CTTAAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 190
BisI GCNGC 8 cut(s) 81, 84, 93, 96, 243, 417, 420, 491
BlsI GCNGC 8 cut(s) 82, 85, 94, 97, 244, 418, 421, 492
Bme1390I CCNGG 3 cut(s) 55, 61, 184
Bme18I GGWCC 1 cut(s) 51
BmgT120I GGNCC 4 cut(s) 51, 57, 68, 69
BmiI GGNNCC 6 cut(s) 34, 40, 52, 58, 70, 465
BmrFI CCNGG 3 cut(s) 55, 61, 184
BmsI GCATC 3 cut(s) 225, 269, 332
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 1 cut(s) 140
BpuEI CTTGAG 2 cut(s) 198, 413
BpuMI CCSGG 2 cut(s) 55, 61
BsaBI GATNNNNATC 1 cut(s) 322
BsaJI CCNNGG 2 cut(s) 54, 183
Bsc4I CCNNNNNNNGG 4 cut(s) 48, 60, 66, 343
Bse8I GATNNNNATC 1 cut(s) 322
BseBI CCWGG 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 54, 183
BseGI GGATG 1 cut(s) 323
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 4 cut(s) 48, 60, 66, 343
BseRI GAGGAG 3 cut(s) 30, 33, 425
BseSI GKGCMC 1 cut(s) 72
BseXI GCAGC 4 cut(s) 107, 229, 406, 428
BsgI GTGCAG 1 cut(s) 288
BshFI GGCC 3 cut(s) 59, 70, 374
BshNI GGYRCC 1 cut(s) 463
BsiSI CCGG 4 cut(s) 36, 42, 54, 60
BslI CCNNNNNNNGG 4 cut(s) 48, 60, 66, 343
BsnI GGCC 3 cut(s) 59, 70, 374
Bsp120I GGGCCC 1 cut(s) 68
Bsp1286I GDGCHC 1 cut(s) 72
BspACI CCGC 5 cut(s) 24, 80, 83, 92, 388
BspANI GGCC 3 cut(s) 59, 70, 374
BspHI TCATGA 1 cut(s) 225
BspLI GGNNCC 6 cut(s) 34, 40, 52, 58, 70, 465
BspT107I GGYRCC 1 cut(s) 463
BspTI CTTAAG 1 cut(s) 275
BssECI CCNNGG 2 cut(s) 54, 183
Bst2UI CCWGG 1 cut(s) 184
BstAFI CTTAAG 1 cut(s) 275
BstC8I GCNNGC 3 cut(s) 240, 372, 424
BstDEI CTNAG 1 cut(s) 99
BstF5I GGATG 1 cut(s) 323
BstMWI GCNNNNNNNGC 2 cut(s) 89, 92
BstNI CCWGG 1 cut(s) 184
BstNSI RCATGY 1 cut(s) 394
BstSCI CCNGG 3 cut(s) 53, 59, 182
BstSLI GKGCMC 1 cut(s) 72
BstV1I GCAGC 4 cut(s) 107, 229, 406, 428
BstV2I GAAGAC 1 cut(s) 386
BsuI GTATCC 1 cut(s) 190
BsuRI GGCC 3 cut(s) 59, 70, 374
BtgZI GCGATG 1 cut(s) 124
BtsCI GGATG 1 cut(s) 323
Cac8I GCNNGC 3 cut(s) 240, 372, 424
CciI TCATGA 1 cut(s) 225
Cfr13I GGNCC 4 cut(s) 51, 57, 68, 69
Csp6I GTAC 1 cut(s) 464
CviAII CATG 4 cut(s) 226, 338, 391, 476
CviQI GTAC 1 cut(s) 464
DdeI CTNAG 1 cut(s) 99
EciI GGCGGA 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 72
Eco47I GGWCC 1 cut(s) 51
Eco57I CTGAAG 1 cut(s) 314
EcoRII CCWGG 1 cut(s) 182
EcoT38I GRGCYC 1 cut(s) 72
FaeI CATG 4 cut(s) 229, 341, 394, 479
FaiI YATR 5 cut(s) 227, 339, 392, 471, 477
FatI CATG 4 cut(s) 225, 337, 390, 475
FblI GTMKAC 1 cut(s) 153
Fnu4HI GCNGC 8 cut(s) 81, 84, 93, 96, 243, 417, 420, 491
FokI GGATG 1 cut(s) 310
FriOI GRGCYC 1 cut(s) 72
Fsp4HI GCNGC 8 cut(s) 81, 84, 93, 96, 243, 417, 420, 491
FspBI CTAG 2 cut(s) 246, 399
GluI GCNGC 8 cut(s) 81, 84, 93, 96, 243, 417, 420, 491
GsuI CTGGAG 1 cut(s) 140
HaeIII GGCC 3 cut(s) 59, 70, 374
HapII CCGG 4 cut(s) 36, 42, 54, 60
Hin1II CATG 4 cut(s) 229, 341, 394, 479
HincII GTYRAC 2 cut(s) 154, 175
HindII GTYRAC 2 cut(s) 154, 175
HindIII AAGCTT 1 cut(s) 219
HinfI GANTC 1 cut(s) 266
HpaII CCGG 4 cut(s) 36, 42, 54, 60
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 2 cut(s) 154, 175
Hpy188I TCNGA 2 cut(s) 265, 289
Hpy188III TCNNGA 3 cut(s) 215, 226, 315
Hpy8I GTNNAC 2 cut(s) 154, 175
Hpy99I CGWCG 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 3 cut(s) 305, 323, 490
HpyF10VI GCNNNNNNNGC 2 cut(s) 89, 92
HpyF3I CTNAG 1 cut(s) 99
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 4 cut(s) 229, 341, 394, 479
KpnI GGTACC 1 cut(s) 467
LmnI GCTCC 4 cut(s) 26, 32, 38, 433
Lsp1109I GCAGC 4 cut(s) 107, 229, 406, 428
LweI GCATC 3 cut(s) 225, 269, 332
MaeI CTAG 2 cut(s) 246, 399
MaeII ACGT 1 cut(s) 177
MboII GAAGA 4 cut(s) 121, 391, 447, 450
MhlI GDGCHC 1 cut(s) 72
MluCI AATT 1 cut(s) 402
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 9 cut(s) 8, 11, 14, 38, 179, 337, 403, 406, 437
MseI TTAA 2 cut(s) 276, 330
MslI CAYNNNNRTG 1 cut(s) 474
MspA1I CMGCKG 1 cut(s) 419
MspCI CTTAAG 1 cut(s) 275
MspI CCGG 4 cut(s) 36, 42, 54, 60
MspR9I CCNGG 3 cut(s) 55, 61, 184
MvaI CCWGG 1 cut(s) 184
MwoI GCNNNNNNNGC 2 cut(s) 89, 92
NciI CCSGG 2 cut(s) 55, 61
NlaIII CATG 4 cut(s) 229, 341, 394, 479
NlaIV GGNNCC 6 cut(s) 34, 40, 52, 58, 70, 465
NmeAIII GCCGAG 1 cut(s) 400
NspI RCATGY 1 cut(s) 394
PagI TCATGA 1 cut(s) 225
PcsI WCGNNNNNNNCGW 2 cut(s) 83, 147
PfeI GAWTC 1 cut(s) 266
PkrI GCNGC 8 cut(s) 82, 85, 94, 97, 244, 418, 421, 492
Psp6I CCWGG 1 cut(s) 182
PspGI CCWGG 1 cut(s) 182
PspN4I GGNNCC 6 cut(s) 34, 40, 52, 58, 70, 465
PspOMI GGGCCC 1 cut(s) 68
PspPI GGNCC 4 cut(s) 51, 57, 68, 69
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 465
RsaNI GTAC 1 cut(s) 464
RseI CAYNNNNRTG 1 cut(s) 474
SalI GTCGAC 1 cut(s) 152
SaqAI TTAA 2 cut(s) 276, 330
SatI GCNGC 8 cut(s) 81, 84, 93, 96, 243, 417, 420, 491
Sau96I GGNCC 4 cut(s) 51, 57, 68, 69
ScrFI CCNGG 3 cut(s) 55, 61, 184
SduI GDGCHC 1 cut(s) 72
SfaNI GCATC 3 cut(s) 225, 269, 332
SinI GGWCC 1 cut(s) 51
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 3 cut(s) 213, 275, 428
SmoI CTYRAG 3 cut(s) 213, 275, 428
Sse9I AATT 1 cut(s) 402
SsiI CCGC 5 cut(s) 24, 80, 83, 92, 388
SspMI CTAG 2 cut(s) 246, 399
StyD4I CCNGG 3 cut(s) 53, 59, 182
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 153
TasI AATT 1 cut(s) 402
TauI GCSGC 3 cut(s) 83, 86, 95
TfiI GAWTC 1 cut(s) 266
Tru1I TTAA 2 cut(s) 276, 330
Tru9I TTAA 2 cut(s) 276, 330
TseI GCWGC 5 cut(s) 95, 242, 416, 419, 490
TspDTI ATGAA 3 cut(s) 17, 214, 492
Vha464I CTTAAG 1 cut(s) 275
VpaK11BI GGWCC 1 cut(s) 51
XceI RCATGY 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 153
XspI CTAG 2 cut(s) 246, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.