MD02G1009700.v1.1

mediator of RNA polymerase II transcription subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012927
Physical Location & Seq
Reverse (-)
641543 .. 643258
1716 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1009700.v1.1.491

Sequence Viewer

Length: 486 bp
ATGATGAGCATTCAGAAGAATATGAACAAGGGCGGAGGTGCAACATCAGGAGGAGGAGGTTTGGGAGGCGCGGGGAGCGGGCCGACCGCGGCTGCTGCGGTGGCGGCAGCTCAGAAGCAGAAGGCACTGCTGCAGAGAGTGGAGACCGACATCGCCAACATCGTCGACAATTTCAGCCACCTCGTCAACGTCTCCAGAGTGAATGATCCTCCTATCAGAAACTCTCAGGAAGCTTTCATGATGGAGATGCGTGCTGCTCGAATGACGGCAATATTTTCAGGGTTTGCCTCCCTCGATGACCACGTAGAGAAAAGAATTGTGGAATTCAACCAGCAAGCCAAGAACACTTCCCGCATGTTGGCTAGAATCGGGGAGGAGGCAGCTGGTAGCCTGAAGGAGCTCGAACCCCATTACTACTCTTCCCCGCTGAGGACTGCTCAAAACGTGCAGCCACGAGCTTGGCATTTACAGTGGAAACTAGTGTAA

Protein Analysis

162

Amino Acids

17.42

Weight (kDa)

9.62

Isoelectric Point (pI)

42.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Med22 PF06179 45 - 89 4.3e-09 Surfeit locus protein 5 subunit 22 of Mediator complex
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012927)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G07950 AT1G07950 AT1G16430
fragaria_vesca FvH4_3g13800
malus_domestica MD02G1009700.v1.1
prunus_persica Prupe.7G260300_v2.0.a1 Prupe.7G260300_v2.0.a1
pyrus_communis pycom15g13880
rosa_chinensis RchiOBHm_Chr5g0022991
rosa_laevigata RLG00000032687
rosa_multiflora Rmu_sc0002675.1_g000006
rosa_roxburghii Rroxscaffold_1G00056350
rosa_rugosa Rorug05G0076300
rosa_samantha Rh5AG162500 Rh5BG161700 Rh5CG176400 Rh5DG163100
rosa_wichuraiana Rw5G014640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 78
AccI GTMKAC 1 cut(s) 165
AccII CGCG 2 cut(s) 71, 89
AciI CCGC 9 cut(s) 33, 71, 78, 87, 89, 98, 104, 352, 425
AclWI GGATC 1 cut(s) 200
AcsI RAATTY 1 cut(s) 323
AcuI CTGAAG 1 cut(s) 413
AfiI CCNNNNNNNGG 2 cut(s) 358, 429
AgsI TTSAA 1 cut(s) 328
AhlI ACTAGT 1 cut(s) 478
AluBI AGCT 5 cut(s) 110, 233, 383, 400, 458
AluI AGCT 5 cut(s) 110, 233, 383, 400, 458
Alw21I GWGCWC 1 cut(s) 402
Alw26I GTCTC 2 cut(s) 137, 196
AlwI GGATC 1 cut(s) 200
AoxI GGCC 1 cut(s) 80
ApeKI GCWGC 7 cut(s) 92, 95, 107, 130, 254, 380, 448
ApoI RAATTY 1 cut(s) 323
AspLEI GCGC 1 cut(s) 71
AspS9I GGNCC 1 cut(s) 80
BanII GRGCYC 1 cut(s) 402
BauI CACGAG 1 cut(s) 453
Bbv12I GWGCWC 1 cut(s) 402
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 7 cut(s) 79, 82, 117, 119, 241, 392, 460
BccI CCATC 1 cut(s) 235
BceAI ACGGC 1 cut(s) 282
BcoDI GTCTC 2 cut(s) 137, 196
BcuI ACTAGT 1 cut(s) 478
BfaI CTAG 2 cut(s) 363, 479
BfmI CTRYAG 1 cut(s) 131
BisI GCNGC 9 cut(s) 90, 93, 96, 105, 108, 131, 255, 381, 449
BlsI GCNGC 9 cut(s) 91, 94, 97, 106, 109, 132, 256, 382, 450
BmgT120I GGNCC 1 cut(s) 80
BmsI GCATC 1 cut(s) 237
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 1 cut(s) 178
Bpu10I CCTNAGC 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 304
BsaI GGTCTC 1 cut(s) 137
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 429
BseDI CCNNGG 1 cut(s) 87
BseLI CCNNNNNNNGG 2 cut(s) 358, 429
BseMII CTCAG 3 cut(s) 125, 239, 419
BseRI GAGGAG 3 cut(s) 66, 69, 389
BseXI GCAGC 7 cut(s) 79, 82, 117, 119, 241, 392, 460
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 2 cut(s) 71, 89
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 1 cut(s) 82
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 402
BslI CCNNNNNNNGG 2 cut(s) 358, 429
BsmAI GTCTC 2 cut(s) 137, 196
BsmBI CGTCTC 1 cut(s) 196
BsmI GAATGC 1 cut(s) 9
BsnI GGCC 1 cut(s) 82
Bso31I GGTCTC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 1 cut(s) 205
BspACI CCGC 9 cut(s) 33, 71, 78, 87, 89, 98, 104, 352, 425
BspANI GGCC 1 cut(s) 82
BspCNI CTCAG 3 cut(s) 124, 238, 420
BspFNI CGCG 2 cut(s) 71, 89
BspHI TCATGA 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 135
BspPI GGATC 1 cut(s) 200
BspTNI GGTCTC 1 cut(s) 137
BsrBI CCGCTC 1 cut(s) 78
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 1 cut(s) 205
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst4CI ACNGT 1 cut(s) 471
Bst6I CTCTTC 1 cut(s) 424
BstBAI YACGTR 1 cut(s) 304
BstC8I GCNNGC 3 cut(s) 80, 252, 336
BstDEI CTNAG 3 cut(s) 111, 225, 428
BstDSI CCRYGG 1 cut(s) 87
BstFNI CGCG 2 cut(s) 71, 89
BstHHI GCGC 1 cut(s) 71
BstKTI GATC 1 cut(s) 208
BstMAI GTCTC 2 cut(s) 137, 196
BstMBI GATC 1 cut(s) 205
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 4 cut(s) 75, 95, 101, 104
BstNSI RCATGY 1 cut(s) 358
BstSFI CTRYAG 1 cut(s) 131
BstUI CGCG 2 cut(s) 71, 89
BstV1I GCAGC 7 cut(s) 79, 82, 117, 119, 241, 392, 460
BstXI CCANNNNNNTGG 1 cut(s) 459
BsuRI GGCC 1 cut(s) 82
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 136
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 125, 476
Cac8I GCNNGC 3 cut(s) 80, 252, 336
CciI TCATGA 1 cut(s) 237
CfoI GCGC 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 80
Cfr42I CCGCGG 1 cut(s) 90
CviAII CATG 2 cut(s) 238, 355
DdeI CTNAG 3 cut(s) 111, 225, 428
DpnI GATC 1 cut(s) 207
DpnII GATC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 424
EarI CTCTTC 1 cut(s) 424
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 400
Eco24I GRGCYC 1 cut(s) 402
Eco31I GGTCTC 1 cut(s) 137
Eco53kI GAGCTC 1 cut(s) 400
Eco57I CTGAAG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 400
EcoRI GAATTC 1 cut(s) 323
EcoT38I GRGCYC 1 cut(s) 402
Esp3I CGTCTC 1 cut(s) 196
FaeI CATG 2 cut(s) 241, 358
FaiI YATR 3 cut(s) 23, 239, 356
FatI CATG 2 cut(s) 237, 354
FauI CCCGC 4 cut(s) 64, 71, 359, 432
FblI GTMKAC 1 cut(s) 165
Fnu4HI GCNGC 9 cut(s) 90, 93, 96, 105, 108, 131, 255, 381, 449
FriOI GRGCYC 1 cut(s) 402
Fsp4HI GCNGC 9 cut(s) 90, 93, 96, 105, 108, 131, 255, 381, 449
FspBI CTAG 2 cut(s) 363, 479
GlaI GCGC 1 cut(s) 70
GluI GCNGC 9 cut(s) 90, 93, 96, 105, 108, 131, 255, 381, 449
GsuI CTGGAG 1 cut(s) 178
HaeIII GGCC 1 cut(s) 82
HhaI GCGC 1 cut(s) 71
Hin1II CATG 2 cut(s) 241, 358
Hin6I GCGC 1 cut(s) 69
HinP1I GCGC 1 cut(s) 69
HincII GTYRAC 2 cut(s) 166, 187
HindII GTYRAC 2 cut(s) 166, 187
HindIII AAGCTT 1 cut(s) 231
HinfI GANTC 1 cut(s) 366
Hpy166II GTNNAC 2 cut(s) 166, 187
Hpy188I TCNGA 3 cut(s) 15, 114, 218
Hpy188III TCNNGA 4 cut(s) 48, 195, 227, 238
Hpy8I GTNNAC 2 cut(s) 166, 187
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 2 cut(s) 115, 388
HpyCH4III ACNGT 1 cut(s) 471
HpyCH4IV ACGT 3 cut(s) 189, 303, 444
HpyCH4V TGCA 3 cut(s) 41, 133, 448
HpyF10VI GCNNNNNNNGC 4 cut(s) 75, 95, 101, 104
HpyF3I CTNAG 3 cut(s) 111, 225, 428
HpySE526I ACGT 3 cut(s) 189, 303, 444
Hsp92II CATG 2 cut(s) 241, 358
HspAI GCGC 1 cut(s) 69
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 1 cut(s) 205
LmnI GCTCC 2 cut(s) 75, 397
LpnPI CCDG 7 cut(s) 33, 208, 212, 264, 344, 369, 404
Lsp1109I GCAGC 7 cut(s) 79, 82, 117, 119, 241, 392, 460
LweI GCATC 1 cut(s) 237
MaeI CTAG 2 cut(s) 363, 479
MaeII ACGT 3 cut(s) 189, 303, 444
MalI GATC 1 cut(s) 207
MbiI CCGCTC 1 cut(s) 78
MboI GATC 1 cut(s) 205
MboII GAAGA 2 cut(s) 28, 411
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 3 cut(s) 169, 315, 323
MspA1I CMGCKG 3 cut(s) 89, 383, 427
Mva1269I GAATGC 1 cut(s) 9
MvnI CGCG 2 cut(s) 71, 89
MwoI GCNNNNNNNGC 4 cut(s) 75, 95, 101, 104
NdeII GATC 1 cut(s) 205
NlaIII CATG 2 cut(s) 241, 358
NspI RCATGY 1 cut(s) 358
PagI TCATGA 1 cut(s) 237
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 300
PctI GAATGC 1 cut(s) 9
PfeI GAWTC 1 cut(s) 366
PkrI GCNGC 9 cut(s) 91, 94, 97, 106, 109, 132, 256, 382, 450
Ppu21I YACGTR 1 cut(s) 304
Psp124BI GAGCTC 1 cut(s) 402
PspPI GGNCC 1 cut(s) 80
PstI CTGCAG 1 cut(s) 135
PvuII CAGCTG 1 cut(s) 383
SacI GAGCTC 1 cut(s) 402
SacII CCGCGG 1 cut(s) 90
SalI GTCGAC 1 cut(s) 164
SatI GCNGC 9 cut(s) 90, 93, 96, 105, 108, 131, 255, 381, 449
Sau3AI GATC 1 cut(s) 205
Sau96I GGNCC 1 cut(s) 80
SduI GDGCHC 1 cut(s) 402
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 131
Sfr303I CCGCGG 1 cut(s) 90
SgrBI CCGCGG 1 cut(s) 90
SpeI ACTAGT 1 cut(s) 478
Sse9I AATT 3 cut(s) 169, 315, 323
SsiI CCGC 9 cut(s) 33, 71, 78, 87, 89, 98, 104, 352, 425
SspI AATATT 1 cut(s) 273
SspMI CTAG 2 cut(s) 363, 479
SstI GAGCTC 1 cut(s) 402
TaaI ACNGT 1 cut(s) 471
TaiI ACGT 3 cut(s) 192, 306, 447
TaqI TCGA 4 cut(s) 165, 259, 294, 402
TaqII GACCGA 1 cut(s) 161
TasI AATT 3 cut(s) 169, 315, 323
TauI GCSGC 2 cut(s) 92, 107
TfiI GAWTC 1 cut(s) 366
TscAI CASTG 2 cut(s) 132, 476
TseI GCWGC 7 cut(s) 92, 95, 107, 130, 254, 380, 448
TspDTI ATGAA 2 cut(s) 38, 226
TspRI CASTG 2 cut(s) 132, 476
XapI RAATTY 1 cut(s) 323
XceI RCATGY 1 cut(s) 358
XmiI GTMKAC 1 cut(s) 165
XspI CTAG 2 cut(s) 363, 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.