AT1G08390

RecQ-mediated genome instability protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0015489
Physical Location & Seq
Forward (+)
2642072 .. 2643285
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G08390.1

Sequence Viewer

Length: 414 bp
ATGGACTACAGCTTAGCGGCGGTGAAGATGCTTTGTTCTCAGCTCCGGGACGCTAAACCGACGCCTTCTCAGAACGCCGCCTCCCTAGGCGGTGTACTTTTTCAACGTGCTTGGTTACAGGGCGTTTTGGTCCCCTTCTCCGGTGGAGGCGGCGATAATTGTCTGGTACTTGATGACGGAACCGGTCTTTTGGAGCTCGGTCTCACCAACGATTTTGCCCTCCGTCAATGGAAATCAGGAATGTACGTGATGGTTGTTGGTGTCTACCAAGTCCGTACTGGGCAGATACCTCTACTTAAGGTTCATAAGATGGTTGACCTGTCGGTGAGCCCAGATCGTGAAGCCATGTGGTATTTAGAAGTCATGGACGCATACAGACTCTTCTATGAGCCCTTGATCCAAGAGTTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
RMI2

Protein Analysis

137

Amino Acids

15.21

Weight (kDa)

4.98

Isoelectric Point (pI)

34.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMI2 PF16100 6 - 125 7.8e-35 RecQ-mediated genome instability protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08390 AT1G08390
fragaria_vesca FvH4_3g30010
malus_domestica MD11G1153700.v1.1
prunus_persica Prupe.6G116400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0055931
rosa_laevigata RLG00000035024 RLG00000035030
rosa_multiflora Rmu_sc0006749.1_g000003
rosa_roxburghii Rroxscaffold_1G00024430
rosa_rugosa Rorug05G0295400
rosa_samantha Rh5AG366900 Rh5BG377100 Rh5CG400400 Rh5DG390600
rosa_wichuraiana Rw5G034400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 264
AciI CCGC 5 cut(s) 17, 20, 78, 90, 150
AclWI GGATC 1 cut(s) 391
AcyI GRCGYC 1 cut(s) 62
AfaI GTAC 4 cut(s) 96, 168, 245, 277
AfiI CCNNNNNNNGG 1 cut(s) 140
AflII CTTAAG 1 cut(s) 296
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 1 cut(s) 104
AloI GAACNNNNNNTCC 2 cut(s) 65, 97
AluBI AGCT 3 cut(s) 12, 43, 196
AluI AGCT 3 cut(s) 12, 43, 196
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 206
AlwI GGATC 1 cut(s) 391
AsiGI ACCGGT 1 cut(s) 182
AspA2I CCTAGG 1 cut(s) 85
AspS9I GGNCC 1 cut(s) 130
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 3 cut(s) 34, 196, 337
AvaII GGWCC 1 cut(s) 130
AvrII CCTAGG 1 cut(s) 85
BanII GRGCYC 3 cut(s) 198, 332, 393
Bbv12I GWGCWC 1 cut(s) 198
BccI CCATC 2 cut(s) 244, 304
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 206
BfaI CTAG 1 cut(s) 86
BfmI CTRYAG 1 cut(s) 7
BfrI CTTAAG 1 cut(s) 296
BisI GCNGC 3 cut(s) 18, 78, 151
BlnI CCTAGG 1 cut(s) 85
BlpI GCTNAGC 1 cut(s) 13
BlsI GCNGC 3 cut(s) 19, 79, 152
Bme1390I CCNGG 1 cut(s) 47
Bme18I GGWCC 1 cut(s) 130
BmgT120I GGNCC 1 cut(s) 130
BmiI GGNNCC 2 cut(s) 132, 181
BmrFI CCNGG 1 cut(s) 47
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 1 cut(s) 18
BmuI ACTGGG 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 13
BpuMI CCSGG 1 cut(s) 47
BsaAI YACGTR 1 cut(s) 247
BsaHI GRCGYC 1 cut(s) 62
BsaI GGTCTC 1 cut(s) 206
BsaJI CCNNGG 1 cut(s) 85
BsaWI WCCGGW 2 cut(s) 140, 182
BsaXI ACNNNNNCTCC 2 cut(s) 65, 95
Bsc4I CCNNNNNNNGG 1 cut(s) 140
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 85
BseLI CCNNNNNNNGG 1 cut(s) 140
BseMII CTCAG 2 cut(s) 53, 83
BseNI ACTGG 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 182
BsiHKAI GWGCWC 1 cut(s) 198
BsiSI CCGG 3 cut(s) 46, 141, 183
BslFI GGGAC 2 cut(s) 62, 116
BslI CCNNNNNNNGG 1 cut(s) 140
BsmAI GTCTC 1 cut(s) 206
BsmFI GGGAC 2 cut(s) 62, 116
Bso31I GGTCTC 1 cut(s) 206
Bsp1286I GDGCHC 3 cut(s) 198, 332, 393
Bsp143I GATC 2 cut(s) 334, 396
Bsp1720I GCTNAGC 1 cut(s) 13
BspACI CCGC 5 cut(s) 17, 20, 78, 90, 150
BspCNI CTCAG 2 cut(s) 52, 82
BspLI GGNNCC 2 cut(s) 132, 181
BspPI GGATC 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 296
BspTNI GGTCTC 1 cut(s) 206
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 1 cut(s) 283
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 1 cut(s) 85
BssMI GATC 2 cut(s) 334, 396
BssNI GRCGYC 1 cut(s) 62
BssT1I CCWWGG 1 cut(s) 85
Bst6I CTCTTC 1 cut(s) 386
BstACI GRCGYC 1 cut(s) 62
BstAFI CTTAAG 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 247
BstDEI CTNAG 3 cut(s) 13, 39, 69
BstKTI GATC 2 cut(s) 337, 399
BstMAI GTCTC 1 cut(s) 206
BstMBI GATC 2 cut(s) 334, 396
BstSCI CCNGG 1 cut(s) 45
BstSFI CTRYAG 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 1 cut(s) 130
CseI GACGC 3 cut(s) 59, 70, 377
Csp6I GTAC 4 cut(s) 95, 167, 244, 276
CspAI ACCGGT 1 cut(s) 182
CviAII CATG 2 cut(s) 346, 364
CviJI RGCY 6 cut(s) 12, 43, 196, 330, 344, 391
CviKI_1 RGCY 6 cut(s) 12, 43, 196, 330, 344, 391
CviQI GTAC 4 cut(s) 95, 167, 244, 276
DdeI CTNAG 3 cut(s) 13, 39, 69
DpnI GATC 2 cut(s) 336, 398
DpnII GATC 2 cut(s) 334, 396
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 85
Eco24I GRGCYC 3 cut(s) 198, 332, 393
Eco31I GGTCTC 1 cut(s) 206
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 196
EcoT14I CCWWGG 1 cut(s) 85
EcoT38I GRGCYC 3 cut(s) 198, 332, 393
ErhI CCWWGG 1 cut(s) 85
FaeI CATG 2 cut(s) 349, 367
FaiI YATR 5 cut(s) 306, 347, 365, 373, 387
FaqI GGGAC 2 cut(s) 62, 116
FatI CATG 2 cut(s) 345, 363
FblI GTMKAC 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 18, 78, 151
FriOI GRGCYC 3 cut(s) 198, 332, 393
Fsp4HI GCNGC 3 cut(s) 18, 78, 151
FspBI CTAG 1 cut(s) 86
GluI GCNGC 3 cut(s) 18, 78, 151
HapII CCGG 3 cut(s) 46, 141, 183
HgaI GACGC 3 cut(s) 59, 70, 377
Hin1I GRCGYC 1 cut(s) 62
Hin1II CATG 2 cut(s) 349, 367
HincII GTYRAC 1 cut(s) 316
HindII GTYRAC 1 cut(s) 316
HinfI GANTC 1 cut(s) 378
HpaII CCGG 3 cut(s) 46, 141, 183
HphI GGTGA 3 cut(s) 34, 196, 337
Hpy166II GTNNAC 3 cut(s) 95, 265, 316
Hpy188I TCNGA 1 cut(s) 72
Hpy188III TCNNGA 3 cut(s) 237, 338, 411
Hpy8I GTNNAC 3 cut(s) 95, 265, 316
Hpy99I CGWCG 1 cut(s) 64
HpyAV CCTTC 2 cut(s) 75, 145
HpyCH4IV ACGT 2 cut(s) 106, 246
HpyF3I CTNAG 3 cut(s) 13, 39, 69
HpySE526I ACGT 2 cut(s) 106, 246
Hsp92I GRCGYC 1 cut(s) 62
Hsp92II CATG 2 cut(s) 349, 367
Kzo9I GATC 2 cut(s) 334, 396
LmnI GCTCC 2 cut(s) 48, 193
LpnPI CCDG 9 cut(s) 59, 104, 149, 154, 196, 222, 264, 332, 345
LweI GCATC 1 cut(s) 18
MaeI CTAG 1 cut(s) 86
MaeII ACGT 2 cut(s) 106, 246
MaeIII GTNAC 1 cut(s) 114
MalI GATC 2 cut(s) 336, 398
MboI GATC 2 cut(s) 334, 396
MboII GAAGA 2 cut(s) 37, 373
MhlI GDGCHC 3 cut(s) 198, 332, 393
MluCI AATT 1 cut(s) 157
MlyI GAGTC 1 cut(s) 372
MnlI CCTC 4 cut(s) 91, 140, 230, 300
MseI TTAA 1 cut(s) 297
MspCI CTTAAG 1 cut(s) 296
MspI CCGG 3 cut(s) 46, 141, 183
MspR9I CCNGG 1 cut(s) 47
NciI CCSGG 1 cut(s) 47
NdeII GATC 2 cut(s) 334, 396
NlaIII CATG 2 cut(s) 349, 367
NlaIV GGNNCC 2 cut(s) 132, 181
PfoI TCCNGGA 1 cut(s) 45
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 3 cut(s) 19, 79, 152
PleI GAGTC 1 cut(s) 372
PpsI GAGTC 1 cut(s) 372
Ppu21I YACGTR 1 cut(s) 247
Psp124BI GAGCTC 1 cut(s) 198
PspN4I GGNNCC 2 cut(s) 132, 181
PspPI GGNCC 1 cut(s) 130
RsaI GTAC 4 cut(s) 96, 168, 245, 277
RsaNI GTAC 4 cut(s) 95, 167, 244, 276
SacI GAGCTC 1 cut(s) 198
SaqAI TTAA 1 cut(s) 297
SatI GCNGC 3 cut(s) 18, 78, 151
Sau3AI GATC 2 cut(s) 334, 396
Sau96I GGNCC 1 cut(s) 130
SchI GAGTC 1 cut(s) 372
ScrFI CCNGG 1 cut(s) 47
SduI GDGCHC 3 cut(s) 198, 332, 393
SetI ASST 8 cut(s) 14, 45, 109, 198, 249, 292, 303, 321
SfaNI GCATC 1 cut(s) 18
SfcI CTRYAG 1 cut(s) 7
SinI GGWCC 1 cut(s) 130
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
Sse9I AATT 1 cut(s) 157
SsiI CCGC 5 cut(s) 17, 20, 78, 90, 150
SspMI CTAG 1 cut(s) 86
SstI GAGCTC 1 cut(s) 198
StyD4I CCNGG 1 cut(s) 45
StyI CCWWGG 1 cut(s) 85
TaiI ACGT 2 cut(s) 109, 249
TaqII GACCGA 1 cut(s) 188
TasI AATT 1 cut(s) 157
TatI WGTACW 1 cut(s) 94
TauI GCSGC 3 cut(s) 20, 80, 153
Tru1I TTAA 1 cut(s) 297
Tru9I TTAA 1 cut(s) 297
TspDTI ATGAA 1 cut(s) 293
TspGWI ACGGA 3 cut(s) 192, 212, 263
Vha464I CTTAAG 1 cut(s) 296
VpaK11BI GGWCC 1 cut(s) 130
XcmI CCANNNNNNNNNTGG 1 cut(s) 275
XmaJI CCTAGG 1 cut(s) 85
XmiI GTMKAC 1 cut(s) 264
XspI CTAG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.