RchiOBHm_Chr5g0055931

RecQ-mediated genome instability protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0015489
Physical Location & Seq
Forward (+)
59309082 .. 59310371
1290 bp
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UTR
Exon/CDS
Intron
PRQ33286

Sequence Viewer

Length: 414 bp
ATGAACTACGAGCTTGCAGCGGTGAAGCTGCTCTACGCCCATCTCAAGCACGCAGTCGAATCCCCTTCCGAGAACGCCATGGACCTCCACGGCATCCTCTTCCAACGCGCCTGGTTACAGGGCATTTTGGTCTGGGCCTCCCCCGACGGCGACCGCTTGCTTCTTGACGACGGCACCGGAGTCATCGAACTCTGCCTCAATCCTGAGTTCCGCCGCCGCGGTTGGAGAATCGGAATGTATGTAATGGCTGTTGGACGCTACACTGTGCGTACAGATGAGCCCCCAATGATCCAGATTCACAAGATGGTTGATCTTTCGGCTTCCCCTGATCGAGAGGCGATGTGGTACCTTGAAGTTTTGGAGGCTTATAAATTCTTCTATCAGCGCATGATTGACGACCCTATGCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
RMI2

Protein Analysis

137

Amino Acids

15.97

Weight (kDa)

5.29

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMI2 PF16100 6 - 123 5.6e-31 RecQ-mediated genome instability protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08390 AT1G08390
fragaria_vesca FvH4_3g30010
malus_domestica MD11G1153700.v1.1
prunus_persica Prupe.6G116400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0055931
rosa_laevigata RLG00000035024 RLG00000035030
rosa_multiflora Rmu_sc0006749.1_g000003
rosa_roxburghii Rroxscaffold_1G00024430
rosa_rugosa Rorug05G0295400
rosa_samantha Rh5AG366900 Rh5BG377100 Rh5CG400400 Rh5DG390600
rosa_wichuraiana Rw5G034400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 369
Acc65I GGTACC 1 cut(s) 345
AccB1I GGYRCC 2 cut(s) 173, 345
AccII CGCG 2 cut(s) 108, 219
AciI CCGC 6 cut(s) 20, 154, 211, 214, 217, 219
AclWI GGATC 1 cut(s) 283
AcsI RAATTY 1 cut(s) 371
AfaI GTAC 2 cut(s) 271, 347
AgsI TTSAA 1 cut(s) 353
AjnI CCWGG 1 cut(s) 110
AluBI AGCT 2 cut(s) 13, 28
AluI AGCT 2 cut(s) 13, 28
AlwI GGATC 1 cut(s) 283
AoxI GGCC 1 cut(s) 135
ApeKI GCWGC 2 cut(s) 17, 28
ApoI RAATTY 1 cut(s) 371
Asp718I GGTACC 1 cut(s) 345
AspLEI GCGC 2 cut(s) 110, 387
AspS9I GGNCC 2 cut(s) 82, 135
AsuHPI GGTGA 1 cut(s) 34
AvaII GGWCC 1 cut(s) 82
BanI GGYRCC 2 cut(s) 173, 345
BanII GRGCYC 1 cut(s) 282
BarI GAAGNNNNNNTAC 2 cut(s) 17, 49
BbvI GCAGC 2 cut(s) 15, 29
BccI CCATC 2 cut(s) 48, 298
BceAI ACGGC 3 cut(s) 106, 163, 187
BciT130I CCWGG 1 cut(s) 112
BisI GCNGC 4 cut(s) 18, 29, 214, 217
BlsI GCNGC 4 cut(s) 19, 30, 215, 218
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 1 cut(s) 82
BmgT120I GGNCC 2 cut(s) 82, 135
BmiI GGNNCC 2 cut(s) 175, 347
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 1 cut(s) 102
BpuEI CTTGAG 1 cut(s) 29
BsaJI CCNNGG 3 cut(s) 78, 88, 217
BsaWI WCCGGW 1 cut(s) 176
BseBI CCWGG 1 cut(s) 112
BseDI CCNNGG 3 cut(s) 78, 88, 217
BseGI GGATG 1 cut(s) 93
BseMII CTCAG 1 cut(s) 195
BseXI GCAGC 2 cut(s) 15, 29
Bsh1236I CGCG 2 cut(s) 108, 219
Bsh1285I CGRYCG 1 cut(s) 154
BshFI GGCC 1 cut(s) 137
BshNI GGYRCC 2 cut(s) 173, 345
BsiEI CGRYCG 1 cut(s) 154
BsiSI CCGG 1 cut(s) 177
BsnI GGCC 1 cut(s) 137
Bsp1286I GDGCHC 1 cut(s) 282
Bsp143I GATC 3 cut(s) 288, 310, 328
Bsp19I CCATGG 1 cut(s) 78
BspACI CCGC 6 cut(s) 20, 154, 211, 214, 217, 219
BspANI GGCC 1 cut(s) 137
BspCNI CTCAG 1 cut(s) 196
BspFNI CGCG 2 cut(s) 108, 219
BspLI GGNNCC 2 cut(s) 175, 347
BspPI GGATC 1 cut(s) 283
BspT107I GGYRCC 2 cut(s) 173, 345
BssECI CCNNGG 3 cut(s) 78, 88, 217
BssMI GATC 3 cut(s) 288, 310, 328
BssT1I CCWWGG 1 cut(s) 78
Bst2UI CCWGG 1 cut(s) 112
Bst4CI ACNGT 1 cut(s) 265
Bst6I CTCTTC 1 cut(s) 104
BstC8I GCNNGC 3 cut(s) 15, 51, 158
BstDEI CTNAG 1 cut(s) 204
BstDSI CCRYGG 3 cut(s) 78, 88, 217
BstF5I GGATG 1 cut(s) 93
BstFNI CGCG 2 cut(s) 108, 219
BstHHI GCGC 2 cut(s) 110, 387
BstKTI GATC 3 cut(s) 291, 313, 331
BstMBI GATC 3 cut(s) 288, 310, 328
BstMCI CGRYCG 1 cut(s) 154
BstNI CCWGG 1 cut(s) 112
BstSCI CCNGG 1 cut(s) 110
BstUI CGCG 2 cut(s) 108, 219
BstV1I GCAGC 2 cut(s) 15, 29
BsuRI GGCC 1 cut(s) 137
BtgI CCRYGG 3 cut(s) 78, 88, 217
BtgZI GCGATG 1 cut(s) 353
BtsCI GGATG 1 cut(s) 93
BtsIMutI CAGTG 1 cut(s) 261
Cac8I GCNNGC 3 cut(s) 15, 51, 158
CfoI GCGC 2 cut(s) 110, 387
Cfr13I GGNCC 2 cut(s) 82, 135
Cfr42I CCGCGG 1 cut(s) 220
CseI GACGC 1 cut(s) 264
Csp6I GTAC 2 cut(s) 270, 346
CviAII CATG 3 cut(s) 79, 388, 407
CviJI RGCY 7 cut(s) 13, 28, 137, 248, 280, 320, 365
CviKI_1 RGCY 7 cut(s) 13, 28, 137, 248, 280, 320, 365
CviQI GTAC 2 cut(s) 270, 346
DdeI CTNAG 1 cut(s) 204
DpnI GATC 3 cut(s) 290, 312, 330
DpnII GATC 3 cut(s) 288, 310, 328
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 200
Eco130I CCWWGG 1 cut(s) 78
Eco24I GRGCYC 1 cut(s) 282
Eco47I GGWCC 1 cut(s) 82
EcoRII CCWGG 1 cut(s) 110
EcoT14I CCWWGG 1 cut(s) 78
EcoT22I ATGCAT 1 cut(s) 408
EcoT38I GRGCYC 1 cut(s) 282
ErhI CCWWGG 1 cut(s) 78
FaeI CATG 3 cut(s) 82, 391, 410
FaiI YATR 6 cut(s) 80, 240, 369, 389, 404, 408
FatI CATG 3 cut(s) 78, 387, 406
Fnu4HI GCNGC 4 cut(s) 18, 29, 214, 217
FokI GGATG 1 cut(s) 80
FriOI GRGCYC 1 cut(s) 282
Fsp4HI GCNGC 4 cut(s) 18, 29, 214, 217
GlaI GCGC 2 cut(s) 109, 386
GluI GCNGC 4 cut(s) 18, 29, 214, 217
HaeIII GGCC 1 cut(s) 137
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 264
HhaI GCGC 2 cut(s) 110, 387
Hin1II CATG 3 cut(s) 82, 391, 410
Hin6I GCGC 2 cut(s) 108, 385
HinP1I GCGC 2 cut(s) 108, 385
HinfI GANTC 4 cut(s) 59, 180, 228, 295
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 2 cut(s) 70, 233
Hpy188III TCNNGA 4 cut(s) 164, 203, 292, 332
Hpy99I CGWCG 2 cut(s) 149, 173
HpyAV CCTTC 1 cut(s) 75
HpyCH4III ACNGT 1 cut(s) 265
HpyCH4V TGCA 2 cut(s) 17, 406
HpyF3I CTNAG 1 cut(s) 204
Hsp92II CATG 3 cut(s) 82, 391, 410
HspAI GCGC 2 cut(s) 108, 385
KpnI GGTACC 1 cut(s) 349
KspI CCGCGG 1 cut(s) 220
Kzo9I GATC 3 cut(s) 288, 310, 328
LpnPI CCDG 8 cut(s) 97, 104, 118, 124, 190, 216, 305, 339
Lsp1109I GCAGC 2 cut(s) 15, 29
LweI GCATC 1 cut(s) 102
MaeIII GTNAC 1 cut(s) 114
MalI GATC 3 cut(s) 290, 312, 330
MboI GATC 3 cut(s) 288, 310, 328
MboII GAAGA 2 cut(s) 91, 367
MhlI GDGCHC 1 cut(s) 282
MluCI AATT 1 cut(s) 371
MlyI GAGTC 1 cut(s) 189
MmeI TCCRAC 3 cut(s) 127, 203, 232
MnlI CCTC 6 cut(s) 95, 107, 148, 206, 328, 355
Mph1103I ATGCAT 1 cut(s) 408
MspA1I CMGCKG 2 cut(s) 20, 219
MspI CCGG 1 cut(s) 177
MspR9I CCNGG 1 cut(s) 112
MvaI CCWGG 1 cut(s) 112
MvnI CGCG 2 cut(s) 108, 219
NcoI CCATGG 1 cut(s) 78
NdeII GATC 3 cut(s) 288, 310, 328
NlaIII CATG 3 cut(s) 82, 391, 410
NlaIV GGNNCC 2 cut(s) 175, 347
NsiI ATGCAT 1 cut(s) 408
PfeI GAWTC 3 cut(s) 59, 228, 295
PkrI GCNGC 4 cut(s) 19, 30, 215, 218
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PsiI TTATAA 1 cut(s) 369
Psp6I CCWGG 1 cut(s) 110
PspGI CCWGG 1 cut(s) 110
PspN4I GGNNCC 2 cut(s) 175, 347
PspPI GGNCC 2 cut(s) 82, 135
RsaI GTAC 2 cut(s) 271, 347
RsaNI GTAC 2 cut(s) 270, 346
SacII CCGCGG 1 cut(s) 220
SatI GCNGC 4 cut(s) 18, 29, 214, 217
Sau3AI GATC 3 cut(s) 288, 310, 328
Sau96I GGNCC 2 cut(s) 82, 135
SchI GAGTC 1 cut(s) 189
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 1 cut(s) 282
SetI ASST 4 cut(s) 15, 30, 87, 351
SfaNI GCATC 1 cut(s) 102
Sfr303I CCGCGG 1 cut(s) 220
SgrBI CCGCGG 1 cut(s) 220
SinI GGWCC 1 cut(s) 82
SmlI CTYRAG 1 cut(s) 44
SmoI CTYRAG 1 cut(s) 44
Sse9I AATT 1 cut(s) 371
SsiI CCGC 6 cut(s) 20, 154, 211, 214, 217, 219
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 78
TaaI ACNGT 1 cut(s) 265
TaqI TCGA 3 cut(s) 57, 186, 331
TasI AATT 1 cut(s) 371
TauI GCSGC 2 cut(s) 216, 219
TfiI GAWTC 3 cut(s) 59, 228, 295
TscAI CASTG 1 cut(s) 268
TseI GCWGC 2 cut(s) 17, 28
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 1 cut(s) 268
VpaK11BI GGWCC 1 cut(s) 82
XapI RAATTY 1 cut(s) 371
Zsp2I ATGCAT 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.