Prupe.6G116400_v2.0.a1

RecQ-mediated genome instability protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0015489
Physical Location & Seq
Reverse (-)
8473653 .. 8476314
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G116400.1

Sequence Viewer

Length: 552 bp
ATGTACTTTGGAAACGACACCGTTGCTTCATTTCTCATGGAATTTGAAATGCCCAATTTGGCTCTCATTTACCTTTTAGATATTCCAAAACGCACTCTCACAAGTTGGGCGTGGTTGCGTTTTGGGTTTCAGGGAGCGAGAGAGATGGACTACAACCTAGCTGCTCTTAAGCTGCTGTGTGTCCAACTGAAAGACGCGCAAGAAACTTCTTCCGAGAACGCCATGGAATTGCACAACATCATATTCCAACGTGCCTGGTTACAGGGCATTTTGGTCGATGTCTCCGCCGACGGAGAGTGCTTGTATCTCGACGACGGCACCGGCGTCATCGAGCTCTCCCTCAGACCCGAGTTTCGCGGCCGCCCTTGGAATATCGGAATGTATGTAATGGTTGTTGGACGCTACATGGTCCGTACGGATGAGCCCCCAATGATCCAGGTTCACAAGATGGTTGATCTTTCGGCTTCCCCTGATCGAGAGGCAATGTGGTACCTTGAAGTTTTGGAGGCATACAAAATGTTCTATCAGCCCTTAATGGAAGGACCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
RMI2

Protein Analysis

184

Amino Acids

21.16

Weight (kDa)

4.62

Isoelectric Point (pI)

45.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08390 AT1G08390
fragaria_vesca FvH4_3g30010
malus_domestica MD11G1153700.v1.1
prunus_persica Prupe.6G116400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0055931
rosa_laevigata RLG00000035024 RLG00000035030
rosa_multiflora Rmu_sc0006749.1_g000003
rosa_roxburghii Rroxscaffold_1G00024430
rosa_rugosa Rorug05G0295400
rosa_samantha Rh5AG366900 Rh5BG377100 Rh5CG400400 Rh5DG390600
rosa_wichuraiana Rw5G034400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 489
AccB1I GGYRCC 2 cut(s) 317, 489
AccII CGCG 2 cut(s) 197, 357
AciI CCGC 3 cut(s) 285, 357, 361
AclWI GGATC 1 cut(s) 427
AcoI YGGCCR 1 cut(s) 358
AcsI RAATTY 1 cut(s) 41
AcyI GRCGYC 1 cut(s) 324
AfaI GTAC 3 cut(s) 5, 415, 491
AflII CTTAAG 1 cut(s) 167
AgsI TTSAA 2 cut(s) 47, 497
AjnI CCWGG 2 cut(s) 254, 435
AluBI AGCT 3 cut(s) 161, 172, 334
AluI AGCT 3 cut(s) 161, 172, 334
Alw21I GWGCWC 1 cut(s) 336
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 427
Ama87I CYCGRG 1 cut(s) 347
AoxI GGCC 1 cut(s) 358
ApeKI GCWGC 2 cut(s) 161, 172
ApoI RAATTY 1 cut(s) 41
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
Asp718I GGTACC 1 cut(s) 489
AspLEI GCGC 1 cut(s) 199
AspS9I GGNCC 2 cut(s) 409, 542
AvaI CYCGRG 1 cut(s) 347
AvaII GGWCC 2 cut(s) 409, 542
BanI GGYRCC 2 cut(s) 317, 489
BanII GRGCYC 2 cut(s) 336, 426
Bbv12I GWGCWC 1 cut(s) 336
BbvI GCAGC 2 cut(s) 148, 159
BccI CCATC 2 cut(s) 139, 442
BceAI ACGGC 1 cut(s) 331
BcgI CGANNNNNNTGC 4 cut(s) 5, 39, 256, 290
BciT130I CCWGG 2 cut(s) 256, 437
BcoDI GTCTC 1 cut(s) 286
BfaI CTAG 1 cut(s) 158
BfrI CTTAAG 1 cut(s) 167
BisI GCNGC 4 cut(s) 162, 173, 358, 361
BlsI GCNGC 4 cut(s) 163, 174, 359, 362
Bme1390I CCNGG 2 cut(s) 256, 437
Bme18I GGWCC 2 cut(s) 409, 542
BmeT110I CYCGRG 1 cut(s) 347
BmgT120I GGNCC 2 cut(s) 409, 542
BmiI GGNNCC 2 cut(s) 319, 491
BmrFI CCNGG 2 cut(s) 256, 437
BsaHI GRCGYC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 222, 365
Bse118I RCCGGY 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 489
BseBI CCWGG 2 cut(s) 256, 437
BseDI CCNNGG 2 cut(s) 222, 365
BseGI GGATG 1 cut(s) 424
BseMI GCAATG 1 cut(s) 489
BseMII CTCAG 1 cut(s) 355
BseX3I CGGCCG 1 cut(s) 358
BseXI GCAGC 2 cut(s) 148, 159
Bsh1236I CGCG 2 cut(s) 197, 357
Bsh1285I CGRYCG 1 cut(s) 361
BshFI GGCC 1 cut(s) 360
BshNI GGYRCC 2 cut(s) 317, 489
BsiEI CGRYCG 1 cut(s) 361
BsiHKAI GWGCWC 1 cut(s) 336
BsiHKCI CYCGRG 1 cut(s) 347
BsiSI CCGG 1 cut(s) 321
BsiWI CGTACG 1 cut(s) 413
BsmAI GTCTC 1 cut(s) 286
BsnI GGCC 1 cut(s) 360
BsoBI CYCGRG 1 cut(s) 347
Bsp1286I GDGCHC 2 cut(s) 336, 426
Bsp143I GATC 3 cut(s) 432, 454, 472
Bsp19I CCATGG 1 cut(s) 222
BspACI CCGC 3 cut(s) 285, 357, 361
BspANI GGCC 1 cut(s) 360
BspCNI CTCAG 1 cut(s) 354
BspFNI CGCG 2 cut(s) 197, 357
BspLI GGNNCC 2 cut(s) 319, 491
BspPI GGATC 1 cut(s) 427
BspT107I GGYRCC 2 cut(s) 317, 489
BspTI CTTAAG 1 cut(s) 167
BsrDI GCAATG 1 cut(s) 489
BsrFI RCCGGY 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 222, 365
BssMI GATC 3 cut(s) 432, 454, 472
BssNI GRCGYC 1 cut(s) 324
BssT1I CCWWGG 2 cut(s) 222, 365
Bst2UI CCWGG 2 cut(s) 256, 437
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 324
BstAFI CTTAAG 1 cut(s) 167
BstDEI CTNAG 1 cut(s) 341
BstDSI CCRYGG 1 cut(s) 222
BstF5I GGATG 1 cut(s) 424
BstFNI CGCG 2 cut(s) 197, 357
BstHHI GCGC 1 cut(s) 199
BstKTI GATC 3 cut(s) 435, 457, 475
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 3 cut(s) 432, 454, 472
BstMCI CGRYCG 1 cut(s) 361
BstNI CCWGG 2 cut(s) 256, 437
BstSCI CCNGG 2 cut(s) 254, 435
BstUI CGCG 2 cut(s) 197, 357
BstV1I GCAGC 2 cut(s) 148, 159
BstZI CGGCCG 1 cut(s) 358
BsuRI GGCC 1 cut(s) 360
BtgI CCRYGG 1 cut(s) 222
BtsCI GGATG 1 cut(s) 424
CciNI GCGGCCGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 199
Cfr10I RCCGGY 1 cut(s) 320
Cfr13I GGNCC 2 cut(s) 409, 542
CseI GACGC 3 cut(s) 203, 313, 408
Csp6I GTAC 3 cut(s) 4, 414, 490
CviAII CATG 3 cut(s) 37, 223, 406
CviJI RGCY 8 cut(s) 62, 161, 172, 334, 360, 424, 464, 529
CviKI_1 RGCY 8 cut(s) 62, 161, 172, 334, 360, 424, 464, 529
CviQI GTAC 3 cut(s) 4, 414, 490
DdeI CTNAG 1 cut(s) 341
DpnI GATC 3 cut(s) 434, 456, 474
DpnII GATC 3 cut(s) 432, 454, 472
EaeI YGGCCR 1 cut(s) 358
EagI CGGCCG 1 cut(s) 358
EciI GGCGGA 1 cut(s) 274
Ecl136II GAGCTC 1 cut(s) 334
EclXI CGGCCG 1 cut(s) 358
Eco130I CCWWGG 2 cut(s) 222, 365
Eco24I GRGCYC 2 cut(s) 336, 426
Eco47I GGWCC 2 cut(s) 409, 542
Eco52I CGGCCG 1 cut(s) 358
Eco53kI GAGCTC 1 cut(s) 334
Eco88I CYCGRG 1 cut(s) 347
EcoICRI GAGCTC 1 cut(s) 334
EcoO109I RGGNCCY 1 cut(s) 542
EcoRII CCWGG 2 cut(s) 254, 435
EcoT14I CCWWGG 2 cut(s) 222, 365
EcoT38I GRGCYC 2 cut(s) 336, 426
ErhI CCWWGG 2 cut(s) 222, 365
FaeI CATG 3 cut(s) 40, 226, 409
FaiI YATR 6 cut(s) 38, 224, 242, 384, 407, 511
FatI CATG 3 cut(s) 36, 222, 405
Fnu4HI GCNGC 4 cut(s) 162, 173, 358, 361
FokI GGATG 1 cut(s) 431
FriOI GRGCYC 2 cut(s) 336, 426
Fsp4HI GCNGC 4 cut(s) 162, 173, 358, 361
FspBI CTAG 1 cut(s) 158
GlaI GCGC 1 cut(s) 198
GluI GCNGC 4 cut(s) 162, 173, 358, 361
HaeIII GGCC 1 cut(s) 360
HapII CCGG 1 cut(s) 321
HgaI GACGC 3 cut(s) 203, 313, 408
HhaI GCGC 1 cut(s) 199
Hin1I GRCGYC 1 cut(s) 324
Hin1II CATG 3 cut(s) 40, 226, 409
Hin6I GCGC 1 cut(s) 197
HinP1I GCGC 1 cut(s) 197
HpaII CCGG 1 cut(s) 321
Hpy166II GTNNAC 1 cut(s) 442
Hpy188I TCNGA 3 cut(s) 214, 344, 377
Hpy188III TCNNGA 2 cut(s) 308, 476
Hpy8I GTNNAC 1 cut(s) 442
Hpy99I CGWCG 3 cut(s) 293, 314, 317
HpyAV CCTTC 1 cut(s) 533
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 250
HpyCH4V TGCA 1 cut(s) 232
HpyF3I CTNAG 1 cut(s) 341
HpySE526I ACGT 1 cut(s) 250
Hsp92I GRCGYC 1 cut(s) 324
Hsp92II CATG 3 cut(s) 40, 226, 409
HspAI GCGC 1 cut(s) 197
KpnI GGTACC 1 cut(s) 493
Kzo9I GATC 3 cut(s) 432, 454, 472
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 8 cut(s) 116, 241, 248, 268, 334, 422, 449, 483
Lsp1109I GCAGC 2 cut(s) 148, 159
MaeI CTAG 1 cut(s) 158
MaeII ACGT 1 cut(s) 250
MaeIII GTNAC 1 cut(s) 258
MalI GATC 3 cut(s) 434, 456, 474
MboI GATC 3 cut(s) 432, 454, 472
MboII GAAGA 1 cut(s) 201
MhlI GDGCHC 2 cut(s) 336, 426
MluCI AATT 3 cut(s) 41, 55, 227
MmeI TCCRAC 3 cut(s) 208, 271, 376
MnlI CCTC 3 cut(s) 350, 472, 499
MseI TTAA 2 cut(s) 168, 533
MspCI CTTAAG 1 cut(s) 167
MspI CCGG 1 cut(s) 321
MspR9I CCNGG 2 cut(s) 256, 437
MvaI CCWGG 2 cut(s) 256, 437
MvnI CGCG 2 cut(s) 197, 357
NcoI CCATGG 1 cut(s) 222
NdeII GATC 3 cut(s) 432, 454, 472
NlaIII CATG 3 cut(s) 40, 226, 409
NlaIV GGNNCC 2 cut(s) 319, 491
NotI GCGGCCGC 1 cut(s) 358
PcsI WCGNNNNNNNCGW 1 cut(s) 321
Pfl23II CGTACG 1 cut(s) 413
PkrI GCNGC 4 cut(s) 163, 174, 359, 362
PpuMI RGGWCCY 1 cut(s) 542
Psp124BI GAGCTC 1 cut(s) 336
Psp5II RGGWCCY 1 cut(s) 542
Psp6I CCWGG 2 cut(s) 254, 435
PspGI CCWGG 2 cut(s) 254, 435
PspLI CGTACG 1 cut(s) 413
PspN4I GGNNCC 2 cut(s) 319, 491
PspPI GGNCC 2 cut(s) 409, 542
PspPPI RGGWCCY 1 cut(s) 542
RsaI GTAC 3 cut(s) 5, 415, 491
RsaNI GTAC 3 cut(s) 4, 414, 490
SacI GAGCTC 1 cut(s) 336
SaqAI TTAA 2 cut(s) 168, 533
SatI GCNGC 4 cut(s) 162, 173, 358, 361
Sau3AI GATC 3 cut(s) 432, 454, 472
Sau96I GGNCC 2 cut(s) 409, 542
ScrFI CCNGG 2 cut(s) 256, 437
SduI GDGCHC 2 cut(s) 336, 426
SetI ASST 9 cut(s) 75, 159, 163, 174, 253, 336, 441, 495, 547
SgrAI CRCCGGYG 1 cut(s) 320
SinI GGWCC 2 cut(s) 409, 542
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 3 cut(s) 41, 55, 227
SsiI CCGC 3 cut(s) 285, 357, 361
SspMI CTAG 1 cut(s) 158
SstI GAGCTC 1 cut(s) 336
StyD4I CCNGG 2 cut(s) 254, 435
StyI CCWWGG 2 cut(s) 222, 365
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 253
TaqI TCGA 4 cut(s) 276, 309, 330, 475
TasI AATT 3 cut(s) 41, 55, 227
TatI WGTACW 1 cut(s) 3
TauI GCSGC 2 cut(s) 360, 363
Tru1I TTAA 2 cut(s) 168, 533
Tru9I TTAA 2 cut(s) 168, 533
TseI GCWGC 2 cut(s) 161, 172
TspDTI ATGAA 1 cut(s) 18
TspGWI ACGGA 3 cut(s) 306, 401, 431
Vha464I CTTAAG 1 cut(s) 167
VpaK11BI GGWCC 2 cut(s) 409, 542
XapI RAATTY 1 cut(s) 41
XspI CTAG 1 cut(s) 158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.