RLG00000035030

RecQ-mediated genome instability protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0015489
Physical Location & Seq
Forward (+)
59546464 .. 59547767
1304 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035030

Sequence Viewer

Length: 540 bp
ATGAGCGTCCTCAGAATCTCTCCTCTGACGAGGACTTCTCCGAGCTTATCGCTCTTGCAGGTCTTTCGTCTCCAAGGCCATGACAAATCTGGAAACCAAACCTCGACGACGGCGGAAAGGGGAGAGATAGAGGAGAGAGAAAGAGAGACGGCGTCGAGCATCGACTACGGCACTGTGGAGAGAGAGAGGAGAGAGACAGTGCTGAGGAATCAAAATCGGGGCATTTTGGTCTGGGCCTCCCCCGATGACGACCGCTTGCTTCTCGACGACGGCACCGGAGTCATCAAACTTTGCCTCACTCCCGGCTTCCGCCGCCGCCCTTGGAGAATCGGAACGTATGTAATGGTTGTTGGACGCTACACTGTCTGTACAGATGAGCCCCCAATGATCCAGATTCACAAGATGGTTGATCTTTCGGCTTCCCCTGATCGAGAGGCGATGTGGTACCTGGAAGTTTTGGAGGCTTATAAATTCTTCTATCAGCGCATGATTGACGACCCTCTGCATGATTTTACTTCAAAGCTCTTTTGGACCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
RMI2

Protein Analysis

180

Amino Acids

20.8

Weight (kDa)

5.96

Isoelectric Point (pI)

44.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMI2 PF16100 73 - 156 1.2e-22 RecQ-mediated genome instability protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08390 AT1G08390
fragaria_vesca FvH4_3g30010
malus_domestica MD11G1153700.v1.1
prunus_persica Prupe.6G116400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0055931
rosa_laevigata RLG00000035024 RLG00000035030
rosa_multiflora Rmu_sc0006749.1_g000003
rosa_roxburghii Rroxscaffold_1G00024430
rosa_rugosa Rorug05G0295400
rosa_samantha Rh5AG366900 Rh5BG377100 Rh5CG400400 Rh5DG390600
rosa_wichuraiana Rw5G034400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 468
Acc36I ACCTGC 1 cut(s) 49
Acc65I GGTACC 1 cut(s) 444
AccB1I GGYRCC 2 cut(s) 272, 444
AciI CCGC 5 cut(s) 113, 253, 310, 313, 316
AclWI GGATC 1 cut(s) 382
AcsI RAATTY 1 cut(s) 470
AcyI GRCGYC 1 cut(s) 152
AfaI GTAC 2 cut(s) 370, 446
AgsI TTSAA 1 cut(s) 519
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 2 cut(s) 45, 523
AluI AGCT 2 cut(s) 45, 523
Alw26I GTCTC 3 cut(s) 74, 140, 188
AlwI GGATC 1 cut(s) 382
AoxI GGCC 2 cut(s) 76, 234
ApoI RAATTY 1 cut(s) 470
Asp718I GGTACC 1 cut(s) 444
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 234, 531
AsuC2I CCSGG 1 cut(s) 303
AvaII GGWCC 1 cut(s) 531
BanI GGYRCC 2 cut(s) 272, 444
BanII GRGCYC 1 cut(s) 381
BbvCI CCTCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 397
BceAI ACGGC 4 cut(s) 126, 165, 184, 286
BcgI CGANNNNNNTGC 2 cut(s) 47, 81
BciT130I CCWGG 1 cut(s) 449
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 3 cut(s) 74, 140, 188
BfuAI ACCTGC 1 cut(s) 49
BisI GCNGC 2 cut(s) 313, 316
BlsI GCNGC 2 cut(s) 314, 317
Bme1390I CCNGG 2 cut(s) 303, 449
Bme18I GGWCC 1 cut(s) 531
BmgT120I GGNCC 2 cut(s) 234, 531
BmiI GGNNCC 2 cut(s) 274, 446
BmrFI CCNGG 2 cut(s) 303, 449
BmsI GCATC 1 cut(s) 168
BplI GAGNNNNNCTC 2 cut(s) 22, 54
Bpu10I CCTNAGC 1 cut(s) 203
BpuMI CCSGG 1 cut(s) 303
BsaHI GRCGYC 1 cut(s) 152
BsaJI CCNNGG 2 cut(s) 73, 320
BsaWI WCCGGW 1 cut(s) 275
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 2 cut(s) 73, 320
BseMII CTCAG 2 cut(s) 25, 194
BseRI GAGGAG 3 cut(s) 12, 146, 202
Bsh1285I CGRYCG 1 cut(s) 253
BshFI GGCC 2 cut(s) 78, 236
BshNI GGYRCC 2 cut(s) 272, 444
BsiEI CGRYCG 1 cut(s) 253
BsiSI CCGG 2 cut(s) 276, 303
BsmAI GTCTC 3 cut(s) 74, 140, 188
BsmBI CGTCTC 2 cut(s) 74, 140
BsnI GGCC 2 cut(s) 78, 236
Bsp1286I GDGCHC 1 cut(s) 381
Bsp1407I TGTACA 1 cut(s) 368
Bsp143I GATC 3 cut(s) 387, 409, 427
BspACI CCGC 5 cut(s) 113, 253, 310, 313, 316
BspANI GGCC 2 cut(s) 78, 236
BspCNI CTCAG 2 cut(s) 24, 195
BspLI GGNNCC 2 cut(s) 274, 446
BspMI ACCTGC 1 cut(s) 49
BspPI GGATC 1 cut(s) 382
BspT107I GGYRCC 2 cut(s) 272, 444
BsrGI TGTACA 1 cut(s) 368
BssECI CCNNGG 2 cut(s) 73, 320
BssMI GATC 3 cut(s) 387, 409, 427
BssNI GRCGYC 1 cut(s) 152
BssT1I CCWWGG 2 cut(s) 73, 320
Bst2UI CCWGG 1 cut(s) 449
Bst4CI ACNGT 3 cut(s) 175, 199, 364
BstACI GRCGYC 1 cut(s) 152
BstAUI TGTACA 1 cut(s) 368
BstC8I GCNNGC 1 cut(s) 257
BstDEI CTNAG 2 cut(s) 11, 203
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 3 cut(s) 390, 412, 430
BstMAI GTCTC 3 cut(s) 74, 140, 188
BstMBI GATC 3 cut(s) 387, 409, 427
BstMCI CGRYCG 1 cut(s) 253
BstMWI GCNNNNNNNGC 1 cut(s) 312
BstNI CCWGG 1 cut(s) 449
BstSCI CCNGG 2 cut(s) 301, 447
BsuRI GGCC 2 cut(s) 78, 236
BtgZI GCGATG 1 cut(s) 452
BtsIMutI CAGTG 3 cut(s) 171, 204, 360
BveI ACCTGC 1 cut(s) 49
Cac8I GCNNGC 1 cut(s) 257
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 2 cut(s) 234, 531
CseI GACGC 2 cut(s) 141, 363
Csp6I GTAC 2 cut(s) 369, 445
CviAII CATG 3 cut(s) 80, 487, 506
CviJI RGCY 8 cut(s) 45, 78, 236, 306, 379, 419, 464, 523
CviKI_1 RGCY 8 cut(s) 45, 78, 236, 306, 379, 419, 464, 523
CviQI GTAC 2 cut(s) 369, 445
DdeI CTNAG 2 cut(s) 11, 203
DpnI GATC 3 cut(s) 389, 411, 429
DpnII GATC 3 cut(s) 387, 409, 427
EciI GGCGGA 2 cut(s) 128, 299
Eco130I CCWWGG 2 cut(s) 73, 320
Eco24I GRGCYC 1 cut(s) 381
Eco47I GGWCC 1 cut(s) 531
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 2 cut(s) 73, 320
EcoT38I GRGCYC 1 cut(s) 381
ErhI CCWWGG 2 cut(s) 73, 320
Esp3I CGTCTC 2 cut(s) 74, 140
FaeI CATG 3 cut(s) 83, 490, 509
FaiI YATR 5 cut(s) 81, 339, 468, 488, 507
FatI CATG 3 cut(s) 79, 486, 505
Fnu4HI GCNGC 2 cut(s) 313, 316
FriOI GRGCYC 1 cut(s) 381
Fsp4HI GCNGC 2 cut(s) 313, 316
GlaI GCGC 1 cut(s) 485
GluI GCNGC 2 cut(s) 313, 316
HaeIII GGCC 2 cut(s) 78, 236
HapII CCGG 2 cut(s) 276, 303
HgaI GACGC 2 cut(s) 141, 363
HhaI GCGC 1 cut(s) 486
Hin1I GRCGYC 1 cut(s) 152
Hin1II CATG 3 cut(s) 83, 490, 509
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HinfI GANTC 5 cut(s) 15, 208, 279, 327, 394
HpaII CCGG 2 cut(s) 276, 303
Hpy188I TCNGA 4 cut(s) 14, 27, 42, 332
Hpy188III TCNNGA 4 cut(s) 90, 263, 391, 431
Hpy99I CGWCG 5 cut(s) 109, 112, 157, 269, 272
HpyCH4III ACNGT 3 cut(s) 175, 199, 364
HpyCH4IV ACGT 1 cut(s) 335
HpyCH4V TGCA 2 cut(s) 58, 505
HpyF10VI GCNNNNNNNGC 1 cut(s) 312
HpyF3I CTNAG 2 cut(s) 11, 203
HpySE526I ACGT 1 cut(s) 335
Hsp92I GRCGYC 1 cut(s) 152
Hsp92II CATG 3 cut(s) 83, 490, 509
HspAI GCGC 1 cut(s) 484
KpnI GGTACC 1 cut(s) 448
Kzo9I GATC 3 cut(s) 387, 409, 427
LpnPI CCDG 9 cut(s) 44, 75, 217, 289, 316, 404, 434, 438, 461
LweI GCATC 1 cut(s) 168
MaeII ACGT 1 cut(s) 335
MalI GATC 3 cut(s) 389, 411, 429
MboI GATC 3 cut(s) 387, 409, 427
MboII GAAGA 1 cut(s) 466
MhlI GDGCHC 1 cut(s) 381
MluCI AATT 1 cut(s) 470
MlyI GAGTC 1 cut(s) 288
MmeI TCCRAC 1 cut(s) 331
MspI CCGG 2 cut(s) 276, 303
MspR9I CCNGG 2 cut(s) 303, 449
MvaI CCWGG 1 cut(s) 449
MwoI GCNNNNNNNGC 1 cut(s) 312
NciI CCSGG 1 cut(s) 303
NdeII GATC 3 cut(s) 387, 409, 427
NlaIII CATG 3 cut(s) 83, 490, 509
NlaIV GGNNCC 2 cut(s) 274, 446
PfeI GAWTC 4 cut(s) 15, 208, 327, 394
PflFI GACNNNGTC 1 cut(s) 151
PkrI GCNGC 2 cut(s) 314, 317
PleI GAGTC 1 cut(s) 287
PpsI GAGTC 1 cut(s) 287
PsiI TTATAA 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 447
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 274, 446
PspPI GGNCC 2 cut(s) 234, 531
PsyI GACNNNGTC 1 cut(s) 151
RsaI GTAC 2 cut(s) 370, 446
RsaNI GTAC 2 cut(s) 369, 445
SatI GCNGC 2 cut(s) 313, 316
Sau3AI GATC 3 cut(s) 387, 409, 427
Sau96I GGNCC 2 cut(s) 234, 531
SchI GAGTC 1 cut(s) 288
ScrFI CCNGG 2 cut(s) 303, 449
SduI GDGCHC 1 cut(s) 381
SetI ASST 6 cut(s) 47, 63, 104, 338, 450, 525
SfaNI GCATC 1 cut(s) 168
SinI GGWCC 1 cut(s) 531
Sse9I AATT 1 cut(s) 470
SsiI CCGC 5 cut(s) 113, 253, 310, 313, 316
StyD4I CCNGG 2 cut(s) 301, 447
StyI CCWWGG 2 cut(s) 73, 320
TaaI ACNGT 3 cut(s) 175, 199, 364
TaiI ACGT 1 cut(s) 338
TaqI TCGA 5 cut(s) 104, 155, 162, 264, 430
TasI AATT 1 cut(s) 470
TatI WGTACW 1 cut(s) 368
TauI GCSGC 2 cut(s) 315, 318
TfiI GAWTC 4 cut(s) 15, 208, 327, 394
TscAI CASTG 3 cut(s) 178, 204, 367
TspRI CASTG 3 cut(s) 178, 204, 367
Tth111I GACNNNGTC 1 cut(s) 151
VpaK11BI GGWCC 1 cut(s) 531
XapI RAATTY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 86
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.