MD11G1153700.v1.1

RecQ-mediated genome instability protein 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0015489
Physical Location & Seq
Reverse (-)
14739836 .. 14740806
971 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1153700.v1.1.491

Sequence Viewer

Length: 399 bp
ATGGACTACAACCTAGCAGCACTGAAGCTGCTGTGCGTCCAACTGAAAGACGCGACCGAAGACACCAAGAACGCCATGGAACTCCACGGCATCGCCTTCCAACGCGCCTGGTTGCAGGGCATTCTGGTCCATGTCTTCGACGACGGAGAGCGCTTGCTCCTCGACGACGGCACCGGAGTCATCGAGCTCTCCCTCAGACCCGAATTGCGCCATTGTACTTGGAAAATCGGAATGTATGTAATGACTGTTGGACGGTACACGCTCCGTAAGAATGAGCCGCCAATGATTCAGGTACACAAGATGGTTGATCTTTCGGATTCCCATGATCGAGAGGCAATGTGGTACCTCGAAGTTATGGAGGCATACAATCTTTTCTATCGGCGCTTAATGGAAGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03460 map03460
Pfam Domains
Protein Families
RMI2

Protein Analysis

133

Amino Acids

15.45

Weight (kDa)

5.48

Isoelectric Point (pI)

34.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RMI2 PF16100 6 - 122 1.2e-34 RecQ-mediated genome instability protein 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015489)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08390 AT1G08390
fragaria_vesca FvH4_3g30010
malus_domestica MD11G1153700.v1.1
prunus_persica Prupe.6G116400_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0055931
rosa_laevigata RLG00000035024 RLG00000035030
rosa_multiflora Rmu_sc0006749.1_g000003
rosa_roxburghii Rroxscaffold_1G00024430
rosa_rugosa Rorug05G0295400
rosa_samantha Rh5AG366900 Rh5BG377100 Rh5CG400400 Rh5DG390600
rosa_wichuraiana Rw5G034400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 342
AccB1I GGYRCC 2 cut(s) 170, 342
AccII CGCG 2 cut(s) 53, 105
AciI CCGC 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 44
AfaI GTAC 4 cut(s) 217, 257, 294, 344
AfeI AGCGCT 1 cut(s) 152
AjnI CCWGG 1 cut(s) 107
AluBI AGCT 2 cut(s) 28, 187
AluI AGCT 2 cut(s) 28, 187
Alw21I GWGCWC 1 cut(s) 189
Aor51HI AGCGCT 1 cut(s) 152
ApeKI GCWGC 2 cut(s) 17, 28
Asp718I GGTACC 1 cut(s) 342
AspLEI GCGC 4 cut(s) 107, 153, 210, 384
AspS9I GGNCC 1 cut(s) 127
AvaII GGWCC 1 cut(s) 127
BanI GGYRCC 2 cut(s) 170, 342
BanII GRGCYC 1 cut(s) 189
BbsI GAAGAC 2 cut(s) 66, 127
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 2 cut(s) 15, 29
BccI CCATC 1 cut(s) 295
BceAI ACGGC 2 cut(s) 103, 184
BciT130I CCWGG 1 cut(s) 109
BfaI CTAG 1 cut(s) 14
BfoI RGCGCY 2 cut(s) 154, 385
BisI GCNGC 3 cut(s) 18, 29, 278
BlsI GCNGC 3 cut(s) 19, 30, 279
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 1 cut(s) 127
BmgT120I GGNCC 1 cut(s) 127
BmiI GGNNCC 2 cut(s) 172, 344
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 1 cut(s) 99
BpiI GAAGAC 2 cut(s) 66, 127
BsaJI CCNNGG 2 cut(s) 75, 85
BsaWI WCCGGW 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 342
BseBI CCWGG 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 75, 85
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 149
BseXI GCAGC 2 cut(s) 15, 29
Bsh1236I CGCG 2 cut(s) 53, 105
Bsh1285I CGRYCG 1 cut(s) 57
BshNI GGYRCC 2 cut(s) 170, 342
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 1 cut(s) 174
BsmI GAATGC 1 cut(s) 120
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 2 cut(s) 307, 325
Bsp19I CCATGG 1 cut(s) 75
BspACI CCGC 1 cut(s) 278
BspCNI CTCAG 1 cut(s) 207
BspFNI CGCG 2 cut(s) 53, 105
BspLI GGNNCC 2 cut(s) 172, 344
BspT107I GGYRCC 2 cut(s) 170, 342
BsrDI GCAATG 1 cut(s) 342
BssECI CCNNGG 2 cut(s) 75, 85
BssMI GATC 2 cut(s) 307, 325
BssT1I CCWWGG 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 109
Bst4CI ACNGT 2 cut(s) 247, 255
BstC8I GCNNGC 1 cut(s) 155
BstDEI CTNAG 1 cut(s) 194
BstDSI CCRYGG 2 cut(s) 75, 85
BstFNI CGCG 2 cut(s) 53, 105
BstH2I RGCGCY 2 cut(s) 154, 385
BstHHI GCGC 4 cut(s) 107, 153, 210, 384
BstKTI GATC 2 cut(s) 310, 328
BstMBI GATC 2 cut(s) 307, 325
BstMCI CGRYCG 1 cut(s) 57
BstNI CCWGG 1 cut(s) 109
BstSCI CCNGG 1 cut(s) 107
BstUI CGCG 2 cut(s) 53, 105
BstV1I GCAGC 2 cut(s) 15, 29
BstV2I GAAGAC 2 cut(s) 66, 127
BtgI CCRYGG 2 cut(s) 75, 85
BtgZI GCGATG 1 cut(s) 76
BtsIMutI CAGTG 1 cut(s) 20
Cac8I GCNNGC 1 cut(s) 155
CfoI GCGC 4 cut(s) 107, 153, 210, 384
Cfr13I GGNCC 1 cut(s) 127
CseI GACGC 2 cut(s) 25, 59
Csp6I GTAC 4 cut(s) 216, 256, 293, 343
CviAII CATG 3 cut(s) 76, 131, 323
CviJI RGCY 3 cut(s) 28, 187, 277
CviKI_1 RGCY 3 cut(s) 28, 187, 277
CviQI GTAC 4 cut(s) 216, 256, 293, 343
DdeI CTNAG 1 cut(s) 194
DpnI GATC 2 cut(s) 309, 327
DpnII GATC 2 cut(s) 307, 325
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 189
Eco47I GGWCC 1 cut(s) 127
Eco47III AGCGCT 1 cut(s) 152
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 1 cut(s) 44
EcoICRI GAGCTC 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 107
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 3 cut(s) 79, 134, 326
FaiI YATR 6 cut(s) 77, 132, 237, 324, 356, 364
FatI CATG 3 cut(s) 75, 130, 322
Fnu4HI GCNGC 3 cut(s) 18, 29, 278
FriOI GRGCYC 1 cut(s) 189
Fsp4HI GCNGC 3 cut(s) 18, 29, 278
FspBI CTAG 1 cut(s) 14
GlaI GCGC 4 cut(s) 106, 152, 209, 383
GluI GCNGC 3 cut(s) 18, 29, 278
HaeII RGCGCY 2 cut(s) 154, 385
HapII CCGG 1 cut(s) 174
HgaI GACGC 2 cut(s) 25, 59
HhaI GCGC 4 cut(s) 107, 153, 210, 384
Hin1II CATG 3 cut(s) 79, 134, 326
Hin6I GCGC 4 cut(s) 105, 151, 208, 382
HinP1I GCGC 4 cut(s) 105, 151, 208, 382
HinfI GANTC 3 cut(s) 177, 286, 317
HpaII CCGG 1 cut(s) 174
Hpy166II GTNNAC 2 cut(s) 258, 295
Hpy188I TCNGA 3 cut(s) 197, 230, 316
Hpy188III TCNNGA 1 cut(s) 329
Hpy8I GTNNAC 2 cut(s) 258, 295
Hpy99I CGWCG 4 cut(s) 143, 146, 167, 170
HpyAV CCTTC 2 cut(s) 106, 386
HpyCH4III ACNGT 2 cut(s) 247, 255
HpyCH4V TGCA 1 cut(s) 115
HpyF3I CTNAG 1 cut(s) 194
Hsp92II CATG 3 cut(s) 79, 134, 326
HspAI GCGC 4 cut(s) 105, 151, 208, 382
KpnI GGTACC 1 cut(s) 346
Kzo9I GATC 2 cut(s) 307, 325
LmnI GCTCC 2 cut(s) 162, 267
LpnPI CCDG 6 cut(s) 94, 101, 110, 121, 187, 275
Lsp1109I GCAGC 2 cut(s) 15, 29
LweI GCATC 1 cut(s) 99
MaeI CTAG 1 cut(s) 14
MalI GATC 2 cut(s) 309, 327
MboI GATC 2 cut(s) 307, 325
MboII GAAGA 2 cut(s) 71, 127
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 1 cut(s) 203
MlyI GAGTC 1 cut(s) 186
MmeI TCCRAC 3 cut(s) 64, 124, 229
MnlI CCTC 5 cut(s) 170, 203, 325, 352, 356
MseI TTAA 1 cut(s) 386
MspI CCGG 1 cut(s) 174
MspR9I CCNGG 1 cut(s) 109
Mva1269I GAATGC 1 cut(s) 120
MvaI CCWGG 1 cut(s) 109
MvnI CGCG 2 cut(s) 53, 105
NcoI CCATGG 1 cut(s) 75
NdeII GATC 2 cut(s) 307, 325
NlaIII CATG 3 cut(s) 79, 134, 326
NlaIV GGNNCC 2 cut(s) 172, 344
PctI GAATGC 1 cut(s) 120
PfeI GAWTC 2 cut(s) 286, 317
PkrI GCNGC 3 cut(s) 19, 30, 279
PleI GAGTC 1 cut(s) 185
PpsI GAGTC 1 cut(s) 185
Psp124BI GAGCTC 1 cut(s) 189
Psp6I CCWGG 1 cut(s) 107
PspGI CCWGG 1 cut(s) 107
PspN4I GGNNCC 2 cut(s) 172, 344
PspPI GGNCC 1 cut(s) 127
RsaI GTAC 4 cut(s) 217, 257, 294, 344
RsaNI GTAC 4 cut(s) 216, 256, 293, 343
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 386
SatI GCNGC 3 cut(s) 18, 29, 278
Sau3AI GATC 2 cut(s) 307, 325
Sau96I GGNCC 1 cut(s) 127
SchI GAGTC 1 cut(s) 186
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 1 cut(s) 189
SetI ASST 5 cut(s) 15, 30, 189, 294, 348
SfaNI GCATC 1 cut(s) 99
SinI GGWCC 1 cut(s) 127
Sse9I AATT 1 cut(s) 203
SsiI CCGC 1 cut(s) 278
SspMI CTAG 1 cut(s) 14
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 2 cut(s) 247, 255
TaqI TCGA 5 cut(s) 138, 162, 183, 328, 348
TaqII GACCGA 1 cut(s) 71
TasI AATT 1 cut(s) 203
TatI WGTACW 1 cut(s) 215
TauI GCSGC 1 cut(s) 280
TfiI GAWTC 2 cut(s) 286, 317
Tru1I TTAA 1 cut(s) 386
Tru9I TTAA 1 cut(s) 386
TscAI CASTG 1 cut(s) 27
TseI GCWGC 2 cut(s) 17, 28
TspGWI ACGGA 2 cut(s) 159, 254
TspRI CASTG 1 cut(s) 27
VpaK11BI GGWCC 1 cut(s) 127
XcmI CCANNNNNNNNNTGG 1 cut(s) 73
XspI CTAG 1 cut(s) 14
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.