AT1G10640

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014180
Physical Location & Seq
Reverse (-)
3515182 .. 3518035
2854 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G10640.1

Sequence Viewer

Length: 1599 bp
ATGATAATGAGAAGAAGTCTAAGTCTGAGAAGCATCACGATGATGATATTAATGGCTGTTTTGGTCTGGTCAGTGACTCTAGAGACCTGCATTGCCAGAAGAGGAAGACATTGGAGACATAGCCACCGAAGCTCTTCTGACTTGTCTGATTCCTTGTCAAACAAGAAACCGAAGAGCCATGGGAACAGTCACCACCACAGTTCTCACAACAACAACCATCACCACAAGTCTAAACCTAAACCGAAACCAAAGCTGAAAACGCCGCCAAAAGCTCACGACAATAACTCTCCGGTGGTTTCAGGGCCACCAAAAGTCCAACCACCGCCTCTTCCGCCTCAAAAGGGATCTCAAGTTTTCAATGTGATGGATTTTGGAGCAAAGGGTGATGGCAAATGTGATGACACTAAGGCGTTTGAAGCGGCTTGGGTAGCAGCTTGCAAAATCGAAGCATCAGTGATGCTTGTACCGCCTGAATACACTTACCTTGTTGGACCGATCTCATTCTCTGGTCCTTATTGTCAAGCTAACATTGTGTTCCAGCTTGATGGTACGATTATAGCTCCAACGGATTCAAAGACATGGGGAAAAGGGTTAATGTGGTGGATTGATTTTACAAAGCTGAAAGGAATTAAAGTACAAGGGAAAGGTGTTATTGATGGAAGAGGCTCTGGTTGGTGGCAACAAGATTCTCCTTTCATTGATAGTGATACCAAACTCATCGTCCCTTTGAACAATTCTGCTAACCAAAACCCTCCTATGCCGATCAGAAGTGAGCTTGATGAGAGAATGCCAAGCATTAAACCAACGGCATTGAGATTCTCTGGGAGTTTTGGTGTGGAAGTGACGGGTATAACGATACAAAACAGTCCTCAGTGTCACCTCAAATTCGACGACTGCGTAGGGGTTGTGGTGCATGACATAGCCGTTTCTTCACCTGGTGACAGTCCAAACACTGATGGAATTCACCTCCAGAACACCAAAGATGTCCTCATTCACAGCACTACTCTCGCTTGCGGAGATGACTGTATCTCGATCCAAACGGGTTGCTCGAACGTGTTTGTGCACAATGTGAACTGTGGACCGGGTCACGGGATCAGCATTGGTAGTCTCGGCAAAGAGGGTACAAAAGCCTGCGTCTCGAACATAACAGTACGAGACGTAGCTATGCACAACACAATGACAGGTGTCCGAATCAAGACATGGCAAGGAGGAGTAGGATCAGTGAAAGGGATAATCTTCTCAAACATTCAGCTCAACCAAGTACAAATTCCAATAACGATAAACCAATTCTACTGTGACCATAGCAAATGCAAGAACCAAACATCAGCAGTAGCAGTGGAAGGAGTGACTTACGAGAGGATAAAAGGAACTTATACCGTGAAACCGGTGCATTTCGCTTGTAGCGATAACTTCCCGTGTGTAGATGTGCAGTTATCGTCGATAGAGCTTAAACCGGTTCAAGAAAAGTATCGAATGTATGATGCTTATTGCTGGCAGACATTTGGTGAGCTCAACACTCCTACTCTTCCTCCCATTGATTGTTTGAAGATTGGGAAGCCCCCGAGAAACAAAGTTCAGTCCGATCACGATGTGTGTTAA

Protein Analysis

532

Amino Acids

58.35

Weight (kDa)

8.87

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 149 - 229 1.6e-08 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 266 - 485 5.8e-68 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 95
AciI CCGC 6 cut(s) 263, 323, 332, 419, 467, 1014
AclWI GGATC 4 cut(s) 352, 1027, 1100, 1225
AcsI RAATTY 3 cut(s) 884, 960, 1266
AdeI CACNNNGTG 3 cut(s) 938, 1069, 1591
AfaI GTAC 6 cut(s) 465, 550, 636, 1123, 1152, 1263
AfiI CCNNNNNNNGG 2 cut(s) 341, 1088
AflIII ACRYGT 1 cut(s) 1053
AgeI ACCGGT 2 cut(s) 1384, 1453
AgsI TTSAA 6 cut(s) 358, 416, 573, 730, 1460, 1546
AjnI CCWGG 1 cut(s) 934
AjuI GAANNNNNNNTTGG 2 cut(s) 1251, 1283
Alw21I GWGCWC 2 cut(s) 1065, 1512
Alw26I GTCTC 5 cut(s) 77, 109, 1112, 1141, 1149
Alw44I GTGCAC 1 cut(s) 1061
AlwI GGATC 4 cut(s) 352, 1027, 1100, 1225
Ama87I CYCGRG 1 cut(s) 1561
AoxI GGCC 1 cut(s) 302
ApaLI GTGCAC 1 cut(s) 1061
ApeKI GCWGC 1 cut(s) 431
ApoI RAATTY 3 cut(s) 884, 960, 1266
ArsI GACNNNNNNTTYG 2 cut(s) 705, 737
AseI ATTAAT 1 cut(s) 50
AsiGI ACCGGT 2 cut(s) 1384, 1453
Asp700I GAANNNNTTC 1 cut(s) 133
AspS9I GGNCC 4 cut(s) 302, 491, 509, 1079
AsuC2I CCSGG 1 cut(s) 1083
AsuHPI GGTGA 8 cut(s) 182, 212, 395, 869, 924, 950, 956, 1517
AvaI CYCGRG 1 cut(s) 1561
AvaII GGWCC 3 cut(s) 491, 509, 1079
BaeGI GKGCMC 1 cut(s) 1065
BanII GRGCYC 1 cut(s) 1512
BbsI GAAGAC 1 cut(s) 112
Bbv12I GWGCWC 2 cut(s) 1065, 1512
BbvI GCAGC 1 cut(s) 443
BccI CCATC 6 cut(s) 225, 358, 380, 539, 650, 950
BceAI ACGGC 2 cut(s) 822, 908
BcgI CGANNNNNNTGC 2 cut(s) 988, 1022
BciT130I CCWGG 1 cut(s) 936
BcnI CCSGG 1 cut(s) 1083
BcoDI GTCTC 5 cut(s) 77, 109, 1112, 1141, 1149
BfaI CTAG 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 3 cut(s) 263, 420, 432
BlsI GCNGC 3 cut(s) 264, 421, 433
Bme1390I CCNGG 2 cut(s) 936, 1083
Bme18I GGWCC 3 cut(s) 491, 509, 1079
BmeT110I CYCGRG 1 cut(s) 1561
BmgT120I GGNCC 4 cut(s) 302, 491, 509, 1079
BmrFI CCNGG 2 cut(s) 936, 1083
BmsI GCATC 4 cut(s) 42, 447, 458, 1471
BoxI GACNNNNGTC 1 cut(s) 1184
BpiI GAAGAC 1 cut(s) 112
BpmI CTGGAG 1 cut(s) 953
BpuEI CTTGAG 1 cut(s) 333
BpuMI CCSGG 1 cut(s) 1083
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 178
BsaWI WCCGGW 3 cut(s) 289, 1384, 1453
BsaXI ACNNNNNCTCC 4 cut(s) 817, 847, 1513, 1543
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 1088
Bse118I RCCGGY 2 cut(s) 1384, 1453
Bse3DI GCAATG 1 cut(s) 90
BseBI CCWGG 1 cut(s) 936
BseDI CCNNGG 1 cut(s) 178
BseLI CCNNNNNNNGG 2 cut(s) 341, 1088
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 2 cut(s) 17, 884
BseRI GAGGAG 1 cut(s) 1224
BseSI GKGCMC 1 cut(s) 1065
BseXI GCAGC 1 cut(s) 443
BsgI GTGCAG 1 cut(s) 1448
BshFI GGCC 1 cut(s) 304
BshTI ACCGGT 2 cut(s) 1384, 1453
BsiHKAI GWGCWC 2 cut(s) 1065, 1512
BsiHKCI CYCGRG 1 cut(s) 1561
BsiSI CCGG 4 cut(s) 290, 1082, 1385, 1454
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 2 cut(s) 341, 1088
BsmAI GTCTC 5 cut(s) 77, 109, 1112, 1141, 1149
BsmBI CGTCTC 2 cut(s) 1141, 1149
BsmFI GGGAC 1 cut(s) 707
BsmI GAATGC 1 cut(s) 792
BsnI GGCC 1 cut(s) 304
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 1561
Bsp1286I GDGCHC 2 cut(s) 1065, 1512
Bsp143I GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
Bsp19I CCATGG 1 cut(s) 178
BspACI CCGC 6 cut(s) 263, 323, 332, 419, 467, 1014
BspANI GGCC 1 cut(s) 304
BspCNI CTCAG 2 cut(s) 18, 883
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 4 cut(s) 352, 1027, 1100, 1225
BspQI GCTCTTC 2 cut(s) 139, 167
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 1384, 1453
BssAI RCCGGY 2 cut(s) 1384, 1453
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
BssT1I CCWWGG 1 cut(s) 178
Bst2UI CCWGG 1 cut(s) 936
Bst4CI ACNGT 9 cut(s) 188, 200, 866, 944, 1025, 1076, 1150, 1295, 1378
Bst6I CTCTTC 6 cut(s) 94, 139, 167, 333, 655, 1530
BstC8I GCNNGC 4 cut(s) 436, 1012, 1132, 1493
BstDEI CTNAG 4 cut(s) 20, 26, 405, 870
BstDSI CCRYGG 1 cut(s) 178
BstKTI GATC 7 cut(s) 347, 498, 765, 1035, 1095, 1220, 1585
BstMAI GTCTC 5 cut(s) 77, 109, 1112, 1141, 1149
BstMBI GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
BstMWI GCNNNNNNNGC 6 cut(s) 129, 259, 331, 416, 428, 466
BstNI CCWGG 1 cut(s) 936
BstPAI GACNNNNGTC 1 cut(s) 1184
BstSCI CCNGG 2 cut(s) 934, 1081
BstSLI GKGCMC 1 cut(s) 1065
BstV1I GCAGC 1 cut(s) 443
BstV2I GAAGAC 1 cut(s) 112
BstX2I RGATCY 1 cut(s) 344
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 344
BsuRI GGCC 1 cut(s) 304
BtgI CCRYGG 1 cut(s) 178
BtsI GCAGTG 1 cut(s) 1341
BtsIMutI CAGTG 6 cut(s) 78, 459, 878, 951, 1227, 1341
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 4 cut(s) 436, 1012, 1132, 1493
Cfr10I RCCGGY 2 cut(s) 1384, 1453
Cfr13I GGNCC 4 cut(s) 302, 491, 509, 1079
CseI GACGC 1 cut(s) 1123
CsiI ACCWGGT 1 cut(s) 934
Csp6I GTAC 6 cut(s) 464, 549, 635, 1122, 1151, 1262
CspAI ACCGGT 2 cut(s) 1384, 1453
CviAII CATG 4 cut(s) 179, 579, 914, 1200
CviQI GTAC 6 cut(s) 464, 549, 635, 1122, 1151, 1262
DdeI CTNAG 4 cut(s) 20, 26, 405, 870
DpnI GATC 7 cut(s) 346, 497, 764, 1034, 1094, 1219, 1584
DpnII GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
DraIII CACNNNGTG 3 cut(s) 938, 1069, 1591
Eam1104I CTCTTC 6 cut(s) 94, 139, 167, 333, 655, 1530
EarI CTCTTC 6 cut(s) 94, 139, 167, 333, 655, 1530
EciI GGCGGA 1 cut(s) 321
Ecl136II GAGCTC 1 cut(s) 1510
Eco130I CCWWGG 1 cut(s) 178
Eco24I GRGCYC 1 cut(s) 1512
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 3 cut(s) 491, 509, 1079
Eco53kI GAGCTC 1 cut(s) 1510
Eco88I CYCGRG 1 cut(s) 1561
EcoICRI GAGCTC 1 cut(s) 1510
EcoRI GAATTC 1 cut(s) 960
EcoRII CCWGG 1 cut(s) 934
EcoT14I CCWWGG 1 cut(s) 178
EcoT38I GRGCYC 1 cut(s) 1512
ErhI CCWWGG 1 cut(s) 178
Esp3I CGTCTC 2 cut(s) 1141, 1149
FaeI CATG 4 cut(s) 182, 582, 917, 1203
FaqI GGGAC 1 cut(s) 707
FatI CATG 4 cut(s) 178, 578, 913, 1199
Fnu4HI GCNGC 3 cut(s) 263, 420, 432
FriOI GRGCYC 1 cut(s) 1512
Fsp4HI GCNGC 3 cut(s) 263, 420, 432
FspBI CTAG 1 cut(s) 80
GluI GCNGC 3 cut(s) 263, 420, 432
GsuI CTGGAG 1 cut(s) 953
HaeIII GGCC 1 cut(s) 304
HapII CCGG 4 cut(s) 290, 1082, 1385, 1454
HgaI GACGC 1 cut(s) 1123
Hin1II CATG 4 cut(s) 182, 582, 917, 1203
HinfI GANTC 6 cut(s) 76, 149, 569, 686, 816, 1191
HpaII CCGG 4 cut(s) 290, 1082, 1385, 1454
HphI GGTGA 8 cut(s) 182, 212, 395, 869, 924, 950, 956, 1517
Hpy166II GTNNAC 3 cut(s) 1063, 1072, 1079
Hpy188I TCNGA 6 cut(s) 27, 139, 148, 767, 1190, 1582
Hpy188III TCNNGA 9 cut(s) 37, 80, 275, 970, 1030, 1138, 1195, 1460, 1586
Hpy8I GTNNAC 3 cut(s) 1063, 1072, 1079
Hpy99I CGWCG 2 cut(s) 893, 1441
HpyAV CCTTC 1 cut(s) 1334
HpyCH4III ACNGT 9 cut(s) 188, 200, 866, 944, 1025, 1076, 1150, 1295, 1378
HpyCH4IV ACGT 2 cut(s) 1053, 1158
HpyCH4V TGCA 8 cut(s) 90, 438, 913, 1063, 1168, 1311, 1390, 1429
HpyF10VI GCNNNNNNNGC 6 cut(s) 129, 259, 331, 416, 428, 466
HpyF3I CTNAG 4 cut(s) 20, 26, 405, 870
HpySE526I ACGT 2 cut(s) 1053, 1158
Hsp92II CATG 4 cut(s) 182, 582, 917, 1203
Kzo9I GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
LguI GCTCTTC 2 cut(s) 139, 167
LmnI GCTCC 2 cut(s) 374, 565
Lsp1109I GCAGC 1 cut(s) 443
LweI GCATC 4 cut(s) 42, 447, 458, 1471
MabI ACCWGGT 1 cut(s) 934
MaeI CTAG 1 cut(s) 80
MaeII ACGT 2 cut(s) 1053, 1158
MaeIII GTNAC 8 cut(s) 73, 188, 841, 875, 938, 1085, 1295, 1345
MalI GATC 7 cut(s) 346, 497, 764, 1034, 1094, 1219, 1584
MboI GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
MflI RGATCY 1 cut(s) 344
MhlI GDGCHC 2 cut(s) 1065, 1512
MluCI AATT 6 cut(s) 627, 733, 884, 960, 1266, 1286
MlyI GAGTC 1 cut(s) 70
MmeI TCCRAC 3 cut(s) 340, 469, 587
MroXI GAANNNNTTC 1 cut(s) 133
MseI TTAA 6 cut(s) 50, 593, 630, 798, 1449, 1597
MslI CAYNNNNRTG 2 cut(s) 38, 41
MspI CCGG 4 cut(s) 290, 1082, 1385, 1454
MspR9I CCNGG 2 cut(s) 936, 1083
Mva1269I GAATGC 1 cut(s) 792
MvaI CCWGG 1 cut(s) 936
MwoI GCNNNNNNNGC 6 cut(s) 129, 259, 331, 416, 428, 466
NciI CCSGG 1 cut(s) 1083
NcoI CCATGG 1 cut(s) 178
NdeII GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
NlaIII CATG 4 cut(s) 182, 582, 917, 1203
NmeAIII GCCGAG 1 cut(s) 1089
NmuCI GTSAC 8 cut(s) 73, 188, 841, 875, 938, 1085, 1295, 1345
PciSI GCTCTTC 2 cut(s) 139, 167
PcsI WCGNNNNNNNCGW 4 cut(s) 851, 894, 1046, 1401
PctI GAATGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 133
PfeI GAWTC 5 cut(s) 149, 569, 686, 816, 1191
PflFI GACNNNGTC 1 cut(s) 1083
PinAI ACCGGT 2 cut(s) 1384, 1453
PkrI GCNGC 3 cut(s) 264, 421, 433
PleI GAGTC 1 cut(s) 70
PpsI GAGTC 1 cut(s) 70
PshAI GACNNNNGTC 1 cut(s) 1184
PshBI ATTAAT 1 cut(s) 50
Psp124BI GAGCTC 1 cut(s) 1512
Psp6I CCWGG 1 cut(s) 934
PspGI CCWGG 1 cut(s) 934
PspPI GGNCC 4 cut(s) 302, 491, 509, 1079
PsuI RGATCY 1 cut(s) 344
PsyI GACNNNGTC 1 cut(s) 1083
RsaI GTAC 6 cut(s) 465, 550, 636, 1123, 1152, 1263
RsaNI GTAC 6 cut(s) 464, 549, 635, 1122, 1151, 1262
RseI CAYNNNNRTG 2 cut(s) 38, 41
SacI GAGCTC 1 cut(s) 1512
SapI GCTCTTC 2 cut(s) 139, 167
SaqAI TTAA 6 cut(s) 50, 593, 630, 798, 1449, 1597
SatI GCNGC 3 cut(s) 263, 420, 432
Sau3AI GATC 7 cut(s) 344, 495, 762, 1032, 1092, 1217, 1582
Sau96I GGNCC 4 cut(s) 302, 491, 509, 1079
SchI GAGTC 1 cut(s) 70
ScrFI CCNGG 2 cut(s) 936, 1083
SduI GDGCHC 2 cut(s) 1065, 1512
SexAI ACCWGGT 1 cut(s) 934
SfaNI GCATC 4 cut(s) 42, 447, 458, 1471
SinI GGWCC 3 cut(s) 491, 509, 1079
SmiMI CAYNNNNRTG 2 cut(s) 38, 41
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 6 cut(s) 627, 733, 884, 960, 1266, 1286
SsiI CCGC 6 cut(s) 263, 323, 332, 419, 467, 1014
SspMI CTAG 1 cut(s) 80
SstI GAGCTC 1 cut(s) 1512
StyD4I CCNGG 2 cut(s) 934, 1081
StyI CCWWGG 1 cut(s) 178
TaaI ACNGT 9 cut(s) 188, 200, 866, 944, 1025, 1076, 1150, 1295, 1378
TaiI ACGT 2 cut(s) 1056, 1161
TaqI TCGA 7 cut(s) 444, 888, 1031, 1049, 1139, 1439, 1471
TaqII GACCGA 1 cut(s) 508
TasI AATT 6 cut(s) 627, 733, 884, 960, 1266, 1286
TatI WGTACW 2 cut(s) 634, 1261
TauI GCSGC 2 cut(s) 265, 422
TfiI GAWTC 5 cut(s) 149, 569, 686, 816, 1191
Tru1I TTAA 6 cut(s) 50, 593, 630, 798, 1449, 1597
Tru9I TTAA 6 cut(s) 50, 593, 630, 798, 1449, 1597
TscAI CASTG 6 cut(s) 78, 459, 878, 958, 1227, 1341
TseFI GTSAC 8 cut(s) 73, 188, 841, 875, 938, 1085, 1295, 1345
TseI GCWGC 1 cut(s) 431
Tsp45I GTSAC 8 cut(s) 73, 188, 841, 875, 938, 1085, 1295, 1345
TspDTI ATGAA 1 cut(s) 685
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 6 cut(s) 78, 459, 878, 958, 1227, 1341
Tth111I GACNNNGTC 1 cut(s) 1083
VneI GTGCAC 1 cut(s) 1061
VpaK11BI GGWCC 3 cut(s) 491, 509, 1079
VspI ATTAAT 1 cut(s) 50
XapI RAATTY 3 cut(s) 884, 960, 1266
XbaI TCTAGA 1 cut(s) 79
XmnI GAANNNNTTC 1 cut(s) 133
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.