AT1G60590

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014180
Physical Location & Seq
Reverse (-)
22314089 .. 22317070
2982 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G60590.1

Sequence Viewer

Length: 1623 bp
ATGATAACGAGAGAGAGTTTAACGCTGAGAAGCATCACAATGATGACTTTAGTGACAATCCTGGTCTGGTCTGCAACTCTAGAGACCTGCATTGCCAGAAGAGGAAGACATTGGAGACACCACCATAGGAGCTCCTCCTCTTTGTCTGATTCCTTGTCCAGCAAGAAACCAAAAAGCCATGAGAACAACCACCACCACATGTCCCATAAGTCAAAACCAAAACCGAAGATGAAAACTCAGCCGCCAAAATCCAACGACGGTTCACCTGTTGTGTCACAGCCACCACAAGTTCAACAACCACCACCACCACATGTCCAACCACCACCAACACCTCTTCCGCTGCAGCCAGTTGAGGACTCTCAACAATTCAATGTGCTTGATTTTGGAGCTAAGGGTGATGGAATGAGTGATGACACTCAGGCGTTTGAAGCTGCATGGGCATCTGCCTGCAAAGTCGAGGCCTCAACGATGATCATACCGCCTGACTACATCTTCCTCGTTGGCCCGATTTCATTCTCGGGTCCTTATTGTCAAGCAAACATTGTATTCCAACTGGAGGGGATGATTGTAGCTCCTACCGATACTGAATCGTGGGGAGGAGGGTTAATGTGGTGGATTGAGTTCACGAAGCTTAGCGGAATTACAATACAAGGTAATGGTGTTATTGATGGAAGAGGCACTGTTTGGTGGCAACAAGATTACCTTTCCGATTATCCTATCGACGATGACTTTAAGCTCATTGTCCCGTTGAACAACTCGGTCCAAGAACGTCCTCCAATGCCGATAAGAAGTGAACTTAATTGGAGAATGCCAAGTATTAAACCAACGGCGCTGAGGTTTTATGGCAGTATCGATGTGACTGTGACGGGTATAACTATACAGAACAGTCCTCAATGTCACCTCAAATTTGATAACTGTGTCAAGGTTTTAGTTCATGACGTGAATGTGTCTTCTCCCGGAGACAGTCCAAACACAGATGGGATTCATCTTCAGAACACCAGAGATGTCATGATTCACACCACCACACTAGCTTGCGGAGACGATTGTATTTCGATTCAAACCGGTTGCTCCAATGTGTATGTTCACAATGTGAATTGTGGACCAGGGCATGGAATCAGCATTGGTAGCCTTGGTAAAGACAGTACCAAAGCCTGTGTCTCCAACATAACAGTCCGAGATGTCGTTATGCACAACACAATGACCGGTGTCCGGATCAAGACTTGGCAGGGAGGTATAGGGTCAGTGAAAGGAATACTTTTCTCAAATATTCAACTCACAGAGGTTCAGCTACCGATAGTGATCGATCAATTCTACTGTGATCACAGCAAATGCATGAACCATACTTCAGCAGTGTCCGTAGAAGGAGTCACATATGAGAAGATACGAGGCACTTACACCGTGAAGCCTGTGCATTTTGCTTGCAGTGACAGTTTCCCTTGCATAGACGTGCAATTATCAGGCATAGAGCTAAAACCAGTGCAACTACAATACCACATGTACGATCCATTTTGTTGGAAAACATTTGGAGAGCTCAATTCCGCAACCGTTCCACCGATTGATTGCTTACAGATCGGAAAGCCAGCGCGAAATGGAGTCCATTCAGATCATGATATATGTTTATGA

Protein Analysis

540

Amino Acids

59.63

Weight (kDa)

6.44

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 153 - 232 4.7e-08 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 273 - 491 2.7e-68 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 95
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccII CGCG 1 cut(s) 1585
AccIII TCCGGA 1 cut(s) 1209
AciI CCGC 6 cut(s) 242, 338, 479, 636, 1035, 1539
AclWI GGATC 2 cut(s) 1220, 1496
AcsI RAATTY 1 cut(s) 905
AcuI CTGAAG 2 cut(s) 974, 1329
AdeI CACNNNGTG 1 cut(s) 1090
AfaI GTAC 2 cut(s) 1144, 1499
AfiI CCNNNNNNNGG 3 cut(s) 556, 1109, 1209
AflIII ACRYGT 3 cut(s) 198, 310, 1494
AgeI ACCGGT 2 cut(s) 1061, 1202
AgsI TTSAA 6 cut(s) 293, 370, 428, 751, 1058, 1271
AjiI CACGTC 2 cut(s) 940, 1447
AjnI CCWGG 2 cut(s) 60, 1102
Alw21I GWGCWC 2 cut(s) 134, 1533
Alw26I GTCTC 5 cut(s) 77, 109, 954, 1032, 1162
AlwI GGATC 2 cut(s) 1220, 1496
Ama87I CYCGRG 1 cut(s) 517
Aor13HI TCCGGA 1 cut(s) 1209
AoxI GGCC 2 cut(s) 459, 502
ApeKI GCWGC 3 cut(s) 340, 343, 431
ApoI RAATTY 1 cut(s) 905
ArsI GACNNNNNNTTYG 2 cut(s) 1140, 1172
AsiGI ACCGGT 2 cut(s) 1061, 1202
AspLEI GCGC 2 cut(s) 832, 1585
AspS9I GGNCC 4 cut(s) 503, 521, 760, 1100
AsuC2I CCSGG 1 cut(s) 957
AsuHPI GGTGA 3 cut(s) 255, 407, 890
AvaI CYCGRG 1 cut(s) 517
AvaII GGWCC 3 cut(s) 521, 760, 1100
BanII GRGCYC 2 cut(s) 134, 1533
BbsI GAAGAC 2 cut(s) 112, 942
Bbv12I GWGCWC 2 cut(s) 134, 1533
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 3 cut(s) 327, 355, 418
BccI CCATC 3 cut(s) 392, 662, 971
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 2 cut(s) 62, 1104
BclI TGATCA 2 cut(s) 471, 1318
BcnI CCSGG 1 cut(s) 957
BcoDI GTCTC 5 cut(s) 77, 109, 954, 1032, 1162
BfaI CTAG 2 cut(s) 80, 1028
BfmI CTRYAG 1 cut(s) 341
BfoI RGCGCY 1 cut(s) 833
BfuAI ACCTGC 1 cut(s) 95
BisI GCNGC 4 cut(s) 242, 341, 344, 432
BlpI GCTNAGC 1 cut(s) 632
BlsI GCNGC 4 cut(s) 243, 342, 345, 433
Bme1390I CCNGG 3 cut(s) 62, 957, 1104
Bme18I GGWCC 3 cut(s) 521, 760, 1100
BmeT110I CYCGRG 1 cut(s) 517
BmgBI CACGTC 2 cut(s) 940, 1447
BmgT120I GGNCC 4 cut(s) 503, 521, 760, 1100
BmiI GGNNCC 1 cut(s) 522
BmrFI CCNGG 3 cut(s) 62, 957, 1104
BmsI GCATC 2 cut(s) 42, 449
BoxI GACNNNNGTC 1 cut(s) 1205
BpiI GAAGAC 2 cut(s) 112, 942
BpmI CTGGAG 1 cut(s) 575
Bpu10I CCTNAGC 2 cut(s) 390, 833
Bpu1102I GCTNAGC 1 cut(s) 632
BpuMI CCSGG 1 cut(s) 957
Bsa29I ATCGAT 2 cut(s) 852, 1302
BsaBI GATNNNNATC 1 cut(s) 1298
BsaI GGTCTC 1 cut(s) 77
BsaJI CCNNGG 2 cut(s) 1103, 1129
BsaWI WCCGGW 3 cut(s) 1061, 1202, 1209
Bsc4I CCNNNNNNNGG 3 cut(s) 556, 1109, 1209
Bse118I RCCGGY 2 cut(s) 1061, 1202
Bse1I ACTGG 3 cut(s) 347, 558, 1475
Bse3DI GCAATG 1 cut(s) 90
Bse8I GATNNNNATC 1 cut(s) 1298
BseAI TCCGGA 1 cut(s) 1209
BseBI CCWGG 2 cut(s) 62, 1104
BseCI ATCGAT 2 cut(s) 852, 1302
BseDI CCNNGG 2 cut(s) 1103, 1129
BseGI GGATG 1 cut(s) 567
BseJI GATNNNNATC 1 cut(s) 1298
BseLI CCNNNNNNNGG 3 cut(s) 556, 1109, 1209
BseMI GCAATG 1 cut(s) 90
BseMII CTCAG 4 cut(s) 17, 251, 431, 824
BseNI ACTGG 3 cut(s) 347, 558, 1475
BseRI GAGGAG 3 cut(s) 124, 127, 612
BseXI GCAGC 3 cut(s) 327, 355, 418
Bsh1236I CGCG 1 cut(s) 1585
BshFI GGCC 2 cut(s) 461, 504
BshTI ACCGGT 2 cut(s) 1061, 1202
BshVI ATCGAT 2 cut(s) 852, 1302
BsiHKAI GWGCWC 2 cut(s) 134, 1533
BsiHKCI CYCGRG 1 cut(s) 517
BsiSI CCGG 4 cut(s) 957, 1062, 1203, 1210
BslFI GGGAC 2 cut(s) 187, 728
BslI CCNNNNNNNGG 3 cut(s) 556, 1109, 1209
BsmAI GTCTC 5 cut(s) 77, 109, 954, 1032, 1162
BsmBI CGTCTC 1 cut(s) 1032
BsmFI GGGAC 2 cut(s) 187, 728
BsmI GAATGC 1 cut(s) 813
BsnI GGCC 2 cut(s) 461, 504
Bso31I GGTCTC 1 cut(s) 77
BsoBI CYCGRG 1 cut(s) 517
Bsp1286I GDGCHC 2 cut(s) 134, 1533
Bsp13I TCCGGA 1 cut(s) 1209
Bsp143I GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
Bsp1720I GCTNAGC 1 cut(s) 632
BspACI CCGC 6 cut(s) 242, 338, 479, 636, 1035, 1539
BspANI GGCC 2 cut(s) 461, 504
BspCNI CTCAG 4 cut(s) 18, 250, 430, 825
BspDI ATCGAT 2 cut(s) 852, 1302
BspEI TCCGGA 1 cut(s) 1209
BspFNI CGCG 1 cut(s) 1585
BspHI TCATGA 3 cut(s) 934, 1008, 1606
BspLI GGNNCC 1 cut(s) 522
BspMAI CTGCAG 1 cut(s) 345
BspMI ACCTGC 1 cut(s) 95
BspPI GGATC 2 cut(s) 1220, 1496
BspTNI GGTCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 90
BsrFI RCCGGY 2 cut(s) 1061, 1202
BsrI ACTGG 3 cut(s) 347, 558, 1475
BssAI RCCGGY 2 cut(s) 1061, 1202
BssECI CCNNGG 2 cut(s) 1103, 1129
BssMI GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
BssT1I CCWWGG 1 cut(s) 1129
Bst2UI CCWGG 2 cut(s) 62, 1104
Bst6I CTCTTC 3 cut(s) 94, 339, 667
BstC8I GCNNGC 4 cut(s) 448, 1033, 1420, 1581
BstDEI CTNAG 6 cut(s) 26, 237, 390, 417, 632, 833
BstF5I GGATG 1 cut(s) 567
BstFNI CGCG 1 cut(s) 1585
BstH2I RGCGCY 1 cut(s) 833
BstHHI GCGC 2 cut(s) 832, 1585
BstKTI GATC 8 cut(s) 474, 1215, 1302, 1306, 1321, 1504, 1572, 1606
BstMAI GTCTC 5 cut(s) 77, 109, 954, 1032, 1162
BstMBI GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
BstMWI GCNNNNNNNGC 3 cut(s) 428, 437, 1125
BstNI CCWGG 2 cut(s) 62, 1104
BstNSI RCATGY 3 cut(s) 202, 314, 1498
BstPAI GACNNNNGTC 1 cut(s) 1205
BstSCI CCNGG 3 cut(s) 60, 955, 1102
BstSFI CTRYAG 1 cut(s) 341
BstUI CGCG 1 cut(s) 1585
BstV1I GCAGC 3 cut(s) 327, 355, 418
BstV2I GAAGAC 2 cut(s) 112, 942
BstXI CCANNNNNNTGG 1 cut(s) 1512
Bsu15I ATCGAT 2 cut(s) 852, 1302
BsuRI GGCC 2 cut(s) 461, 504
BsuTUI ATCGAT 2 cut(s) 852, 1302
BtrI CACGTC 2 cut(s) 940, 1447
BtsCI GGATG 1 cut(s) 567
BtsI GCAGTG 2 cut(s) 1356, 1429
BtsIMutI CAGTG 5 cut(s) 678, 1248, 1356, 1429, 1482
BveI ACCTGC 1 cut(s) 95
Cac8I GCNNGC 4 cut(s) 448, 1033, 1420, 1581
CciI TCATGA 3 cut(s) 934, 1008, 1606
CfoI GCGC 2 cut(s) 832, 1585
Cfr10I RCCGGY 2 cut(s) 1061, 1202
Cfr13I GGNCC 4 cut(s) 503, 521, 760, 1100
ClaI ATCGAT 2 cut(s) 852, 1302
Csp6I GTAC 2 cut(s) 1143, 1498
CspAI ACCGGT 2 cut(s) 1061, 1202
CviQI GTAC 2 cut(s) 1143, 1498
DdeI CTNAG 6 cut(s) 26, 237, 390, 417, 632, 833
DpnI GATC 8 cut(s) 473, 1214, 1301, 1305, 1320, 1503, 1571, 1605
DpnII GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
DraIII CACNNNGTG 1 cut(s) 1090
Eam1104I CTCTTC 3 cut(s) 94, 339, 667
EarI CTCTTC 3 cut(s) 94, 339, 667
Ecl136II GAGCTC 2 cut(s) 132, 1531
Eco130I CCWWGG 1 cut(s) 1129
Eco147I AGGCCT 1 cut(s) 461
Eco24I GRGCYC 2 cut(s) 134, 1533
Eco31I GGTCTC 1 cut(s) 77
Eco47I GGWCC 3 cut(s) 521, 760, 1100
Eco53kI GAGCTC 2 cut(s) 132, 1531
Eco57I CTGAAG 2 cut(s) 974, 1329
Eco88I CYCGRG 1 cut(s) 517
EcoICRI GAGCTC 2 cut(s) 132, 1531
EcoO109I RGGNCCY 1 cut(s) 521
EcoRII CCWGG 2 cut(s) 60, 1102
EcoT14I CCWWGG 1 cut(s) 1129
EcoT22I ATGCAT 1 cut(s) 1334
EcoT38I GRGCYC 2 cut(s) 134, 1533
ErhI CCWWGG 1 cut(s) 1129
Esp3I CGTCTC 1 cut(s) 1032
FalI AAGNNNNNCTT 4 cut(s) 687, 719, 1239, 1271
FaqI GGGAC 2 cut(s) 187, 728
FauNDI CATATG 1 cut(s) 1372
FbaI TGATCA 2 cut(s) 471, 1318
Fnu4HI GCNGC 4 cut(s) 242, 341, 344, 432
FokI GGATG 1 cut(s) 574
FriOI GRGCYC 2 cut(s) 134, 1533
Fsp4HI GCNGC 4 cut(s) 242, 341, 344, 432
FspBI CTAG 2 cut(s) 80, 1028
GlaI GCGC 2 cut(s) 831, 1584
GluI GCNGC 4 cut(s) 242, 341, 344, 432
GsuI CTGGAG 1 cut(s) 575
HaeII RGCGCY 1 cut(s) 833
HaeIII GGCC 2 cut(s) 461, 504
HapII CCGG 4 cut(s) 957, 1062, 1203, 1210
HhaI GCGC 2 cut(s) 832, 1585
Hin6I GCGC 2 cut(s) 830, 1583
HinP1I GCGC 2 cut(s) 830, 1583
HindIII AAGCTT 1 cut(s) 629
HinfI GANTC 9 cut(s) 149, 356, 587, 982, 1012, 1054, 1113, 1365, 1593
HpaII CCGG 4 cut(s) 957, 1062, 1203, 1210
HphI GGTGA 3 cut(s) 255, 407, 890
Hpy166II GTNNAC 5 cut(s) 263, 624, 794, 1084, 1100
Hpy188I TCNGA 6 cut(s) 148, 709, 993, 1175, 1574, 1603
Hpy188III TCNNGA 7 cut(s) 80, 625, 935, 1009, 1210, 1216, 1607
Hpy8I GTNNAC 5 cut(s) 263, 624, 794, 1084, 1100
Hpy99I CGWCG 2 cut(s) 260, 725
HpyAV CCTTC 1 cut(s) 1355
HpyCH4IV ACGT 3 cut(s) 769, 939, 1446
HpyF10VI GCNNNNNNNGC 3 cut(s) 428, 437, 1125
HpyF3I CTNAG 6 cut(s) 26, 237, 390, 417, 632, 833
HpySE526I ACGT 3 cut(s) 769, 939, 1446
HspAI GCGC 2 cut(s) 830, 1583
Kpn2I TCCGGA 1 cut(s) 1209
Ksp22I TGATCA 2 cut(s) 471, 1318
Kzo9I GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
LmnI GCTCC 5 cut(s) 129, 137, 386, 577, 1073
Lsp1109I GCAGC 3 cut(s) 327, 355, 418
LweI GCATC 2 cut(s) 42, 449
MaeI CTAG 2 cut(s) 80, 1028
MaeII ACGT 3 cut(s) 769, 939, 1446
MaeIII GTNAC 7 cut(s) 52, 273, 856, 862, 896, 1366, 1424
MalI GATC 8 cut(s) 473, 1214, 1301, 1305, 1320, 1503, 1571, 1605
MboI GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
MboII GAAGA 9 cut(s) 111, 117, 238, 326, 484, 684, 942, 980, 1390
MhlI GDGCHC 2 cut(s) 134, 1533
MluCI AATT 8 cut(s) 365, 639, 799, 905, 1093, 1307, 1451, 1534
MlyI GAGTC 3 cut(s) 350, 1374, 1602
MmeI TCCRAC 5 cut(s) 276, 340, 574, 1185, 1493
Mph1103I ATGCAT 1 cut(s) 1334
MroI TCCGGA 1 cut(s) 1209
MseI TTAA 5 cut(s) 20, 605, 732, 798, 819
MslI CAYNNNNRTG 3 cut(s) 38, 41, 1445
MspA1I CMGCKG 1 cut(s) 340
MspI CCGG 4 cut(s) 957, 1062, 1203, 1210
MspR9I CCNGG 3 cut(s) 62, 957, 1104
Mva1269I GAATGC 1 cut(s) 813
MvaI CCWGG 2 cut(s) 62, 1104
MvnI CGCG 1 cut(s) 1585
MwoI GCNNNNNNNGC 3 cut(s) 428, 437, 1125
NciI CCSGG 1 cut(s) 957
NdeI CATATG 1 cut(s) 1372
NdeII GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
NlaIV GGNNCC 1 cut(s) 522
NmuCI GTSAC 7 cut(s) 52, 273, 856, 862, 896, 1366, 1424
NsiI ATGCAT 1 cut(s) 1334
NspI RCATGY 3 cut(s) 202, 314, 1498
PagI TCATGA 3 cut(s) 934, 1008, 1606
PceI AGGCCT 1 cut(s) 461
PciI ACATGT 3 cut(s) 198, 310, 1494
PctI GAATGC 1 cut(s) 813
PfeI GAWTC 6 cut(s) 149, 587, 982, 1012, 1054, 1113
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 955
PinAI ACCGGT 2 cut(s) 1061, 1202
PkrI GCNGC 4 cut(s) 243, 342, 345, 433
PleI GAGTC 3 cut(s) 350, 1373, 1601
PpsI GAGTC 3 cut(s) 350, 1373, 1601
PpuMI RGGWCCY 1 cut(s) 521
PscI ACATGT 3 cut(s) 198, 310, 1494
PshAI GACNNNNGTC 1 cut(s) 1205
Psp124BI GAGCTC 2 cut(s) 134, 1533
Psp5II RGGWCCY 1 cut(s) 521
Psp6I CCWGG 2 cut(s) 60, 1102
PspGI CCWGG 2 cut(s) 60, 1102
PspN4I GGNNCC 1 cut(s) 522
PspPI GGNCC 4 cut(s) 503, 521, 760, 1100
PspPPI RGGWCCY 1 cut(s) 521
PstI CTGCAG 1 cut(s) 345
RsaI GTAC 2 cut(s) 1144, 1499
RsaNI GTAC 2 cut(s) 1143, 1498
RseI CAYNNNNRTG 3 cut(s) 38, 41, 1445
SacI GAGCTC 2 cut(s) 134, 1533
SaqAI TTAA 5 cut(s) 20, 605, 732, 798, 819
SatI GCNGC 4 cut(s) 242, 341, 344, 432
Sau3AI GATC 8 cut(s) 471, 1212, 1299, 1303, 1318, 1501, 1569, 1603
Sau96I GGNCC 4 cut(s) 503, 521, 760, 1100
SchI GAGTC 3 cut(s) 350, 1374, 1602
ScrFI CCNGG 3 cut(s) 62, 957, 1104
SduI GDGCHC 2 cut(s) 134, 1533
SfaNI GCATC 2 cut(s) 42, 449
SfcI CTRYAG 1 cut(s) 341
SinI GGWCC 3 cut(s) 521, 760, 1100
SmiMI CAYNNNNRTG 3 cut(s) 38, 41, 1445
Sse9I AATT 8 cut(s) 365, 639, 799, 905, 1093, 1307, 1451, 1534
SseBI AGGCCT 1 cut(s) 461
SsiI CCGC 6 cut(s) 242, 338, 479, 636, 1035, 1539
SspI AATATT 1 cut(s) 1267
SspMI CTAG 2 cut(s) 80, 1028
SstI GAGCTC 2 cut(s) 134, 1533
StuI AGGCCT 1 cut(s) 461
StyD4I CCNGG 3 cut(s) 60, 955, 1102
StyI CCWWGG 1 cut(s) 1129
TaiI ACGT 3 cut(s) 772, 942, 1449
TaqI TCGA 5 cut(s) 456, 720, 852, 1052, 1302
TaqII GACCGA 1 cut(s) 748
TasI AATT 8 cut(s) 365, 639, 799, 905, 1093, 1307, 1451, 1534
TauI GCSGC 1 cut(s) 244
TfiI GAWTC 6 cut(s) 149, 587, 982, 1012, 1054, 1113
Tru1I TTAA 5 cut(s) 20, 605, 732, 798, 819
Tru9I TTAA 5 cut(s) 20, 605, 732, 798, 819
TscAI CASTG 5 cut(s) 685, 1248, 1356, 1429, 1482
TseFI GTSAC 7 cut(s) 52, 273, 856, 862, 896, 1366, 1424
TseI GCWGC 3 cut(s) 340, 343, 431
Tsp45I GTSAC 7 cut(s) 52, 273, 856, 862, 896, 1366, 1424
TspDTI ATGAA 5 cut(s) 245, 501, 923, 974, 1349
TspGWI ACGGA 1 cut(s) 1345
TspRI CASTG 5 cut(s) 685, 1248, 1356, 1429, 1482
Van91I CCANNNNNTGG 1 cut(s) 1109
VpaK11BI GGWCC 3 cut(s) 521, 760, 1100
XapI RAATTY 1 cut(s) 905
XbaI TCTAGA 1 cut(s) 79
XceI RCATGY 3 cut(s) 202, 314, 1498
XspI CTAG 2 cut(s) 80, 1028
Zsp2I ATGCAT 1 cut(s) 1334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.