FvH4_4g34120

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0014180
Physical Location & Seq
Forward (+)
32079110 .. 32083368
4259 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g34120.t3

Sequence Viewer

Length: 1668 bp
ATGAGTGGGATTAGCTTCAAAAGCCTCACATTCATGCTTCTAGTCGCCTTCCTCGTTTGGTCGTCGAATTTCGAGGCTTGCATGGGCAGAAGAGGAAGGCATTGGAGGCACAGCAGAGTTGGCTCGTCTTCTCTTACAAAGAAAAAAGGGAAAACATCTCATAGTGGTGGTAGCAGCAGTCACCACCATAACAGCGGTAGCAGCGGATCAAAACCAAAGCCGAAACTGAAACCAAAACCTAAAGCTCCGTCTCATAACCAGGCGCCAACTCCATCTCATAACCAGGCACCAACTCCACCCCATAACAAGGCACCATTGCCGGCCCCGGTCCCCAAGCCGAAGGCACCAACTCCACCCCATAACAAGACGCCATTGCCGGCCCCGGTCCCCAAGCCGAGGGACGACGATCAAGCTTACACCTATAATGTGCTGGATTTTGGTGCTAAGGGTGATGGCAGCTCCGATGACACAAAGGCATTCCAAGGTGCATGGGCTGCCGCCTGTAAGGTTGAGGCATCAAAGATCATCGTTCCGGCCGATTACCAGTTTCTCGTGGGCCCCATCTCATTCTCCGGCCCATACTGCCAACCTAACATCTTATTTCAGCTTGATGGCACAATAGTAGCTCCATCAGACACCAAATGGGGCAAAGGTCTGTTTCAATGGCTCGAATTCACTAAGCTAAGAGGGATAACAGTTCAGGGGAAGGGTGTGATCGACGGAAGAGGCTCGCCTTGGTGGCAAGATACTCCCTACGGAGACCCTTATGATGATGAACTAAAACTCATTATACCACTAAACAGCACACACCCGCCATTGATACCGATAAGGAATGAGCTAAATGGCAAAATTTCAGGCATGAAACCAACAGCACTAAGGTTTTACGGGAGTTATAACGTGACCGTCACAGGCATCACGATTCAGAATAGCCCCCAATGCCATCTCAAGTTTGACAACTGCGATGGAGTCTTGGTGCACGATATGTCTGTCTCGTCTCCTGGTGACAGTCCTAATACTGATGGGATTCACCTACAGAATTCCAAAGATGTGCTCATCCATAGCTCCACTCTTGCTTGCGGAGATGACTGTATTTCAATACAAACTGGATGCTCAAATGTATACGTACACAACGTGAACTGTGGACCAGGGCATGGAATCAGCATCGGCAGTTTGGGGAAGGACAACACCAAGGCCTGTGTGTCAAACATCACCGTCCGAGACATTGTCATGCACAATACAATGAACGGTGTAAGAGTCAAGACATGGCAGGGTGGATCAGGGTCAGTTCAGGGAGTCCTATTCTCAAACATCCAAGTGAATGAAGTTCAACTTCCTATTGTAATAGACCAGTTCTACTGCGACAAAAGCACCTGCAGAAACCAATCATCAGCCGTGGCGCTCTCTGGAATCACCTACGAGAGAATCCGAGGGACATACACAGTCAAGCCAGTACATTTCGCCTGCAGCGACAGCCTACCCTGCGTAGACGTGACACTAAACGGCATACAGCTGAAACCAGTACAACAACGCTACCACATGTATGATCCCTTCTGCTGGCAGACATTCGGGGAGTTAAGGACCCCGACGCTGCCACCAATCTCGTGTTTACAAATTGGCAAGCCGGCAAACAACAAGGTCAATTCAGATCATGATGCCTGTGACCTTTAG

Protein Analysis

556

Amino Acids

60.18

Weight (kDa)

8.94

Isoelectric Point (pI)

41.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 170 - 249 6.4e-09 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 287 - 359 8.4e-15 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 894
AarI CACCTGC 1 cut(s) 1379
Acc36I ACCTGC 1 cut(s) 1379
AccB1I GGYRCC 4 cut(s) 262, 286, 310, 343
AccB7I CCANNNNNTGG 1 cut(s) 1151
AccI GTMKAC 2 cut(s) 1119, 1485
AciI CCGC 5 cut(s) 195, 204, 498, 812, 1077
AclWI GGATC 3 cut(s) 214, 1282, 1538
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 4 cut(s) 67, 671, 849, 1036
AcyI GRCGYC 2 cut(s) 263, 368
AdeI CACNNNGTG 1 cut(s) 1132
AfaI GTAC 3 cut(s) 1125, 1452, 1521
AfiI CCNNNNNNNGG 4 cut(s) 307, 396, 1151, 1554
AflIII ACRYGT 1 cut(s) 1536
AgsI TTSAA 4 cut(s) 19, 662, 1095, 1328
AjiI CACGTC 1 cut(s) 1489
AjnI CCWGG 4 cut(s) 258, 282, 997, 1144
Alw21I GWGCWC 2 cut(s) 978, 1053
Alw26I GTCTC 5 cut(s) 255, 753, 994, 999, 1212
Alw44I GTGCAC 1 cut(s) 974
AlwI GGATC 3 cut(s) 214, 1282, 1538
AoxI GGCC 6 cut(s) 321, 378, 534, 556, 574, 1191
ApaI GGGCCC 1 cut(s) 560
ApaLI GTGCAC 1 cut(s) 974
ApeKI GCWGC 6 cut(s) 174, 201, 456, 494, 1464, 1588
ApoI RAATTY 4 cut(s) 67, 671, 849, 1036
AspLEI GCGC 2 cut(s) 265, 1399
AspS9I GGNCC 9 cut(s) 322, 328, 379, 385, 556, 557, 575, 1142, 1578
AsuC2I CCSGG 2 cut(s) 326, 383
AsuHPI GGTGA 6 cut(s) 173, 461, 1013, 1019, 1201, 1402
AvaII GGWCC 4 cut(s) 328, 385, 1142, 1578
BaeGI GKGCMC 2 cut(s) 560, 978
BanI GGYRCC 4 cut(s) 262, 286, 310, 343
BanII GRGCYC 1 cut(s) 560
BarI GAAGNNNNNNTAC 2 cut(s) 1532, 1564
BauI CACGAG 2 cut(s) 551, 1600
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 978, 1053
BbvI GCAGC 6 cut(s) 186, 213, 468, 481, 1476, 1575
BccI CCATC 8 cut(s) 280, 446, 569, 605, 637, 948, 956, 1013
BceAI ACGGC 2 cut(s) 1376, 1516
BciT130I CCWGG 4 cut(s) 260, 284, 999, 1146
BcnI CCSGG 2 cut(s) 326, 383
BcoDI GTCTC 5 cut(s) 255, 753, 994, 999, 1212
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 3 cut(s) 1031, 1372, 1462
BfoI RGCGCY 2 cut(s) 266, 1400
BfuAI ACCTGC 1 cut(s) 1379
BglI GCCNNNNNGGC 1 cut(s) 739
BisI GCNGC 7 cut(s) 175, 202, 457, 495, 498, 1465, 1589
BlsI GCNGC 7 cut(s) 176, 203, 458, 496, 499, 1466, 1590
Bme1390I CCNGG 6 cut(s) 260, 284, 326, 383, 999, 1146
Bme18I GGWCC 4 cut(s) 328, 385, 1142, 1578
BmgBI CACGTC 1 cut(s) 1489
BmgT120I GGNCC 9 cut(s) 322, 328, 379, 385, 556, 557, 575, 1142, 1578
BmrFI CCNGG 6 cut(s) 260, 284, 326, 383, 999, 1146
BmsI GCATC 5 cut(s) 524, 921, 1097, 1170, 1642
BpiI GAAGAC 1 cut(s) 120
Bpu10I CCTNAGC 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 929
BpuMI CCSGG 2 cut(s) 326, 383
BsaAI YACGTR 1 cut(s) 1123
BsaHI GRCGYC 2 cut(s) 263, 368
BsaI GGTCTC 1 cut(s) 753
BsaJI CCNNGG 9 cut(s) 324, 381, 395, 481, 734, 1145, 1188, 1392, 1426
BsaXI ACNNNNNCTCC 2 cut(s) 957, 987
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 396, 1151, 1554
Bse118I RCCGGY 3 cut(s) 319, 376, 1621
Bse1I ACTGG 5 cut(s) 544, 1108, 1348, 1448, 1517
Bse3DI GCAATG 2 cut(s) 314, 371
BseBI CCWGG 4 cut(s) 260, 284, 999, 1146
BseDI CCNNGG 9 cut(s) 324, 381, 395, 481, 734, 1145, 1188, 1392, 1426
BseGI GGATG 3 cut(s) 1053, 1112, 1308
BseLI CCNNNNNNNGG 4 cut(s) 307, 396, 1151, 1554
BseMI GCAATG 2 cut(s) 314, 371
BseNI ACTGG 5 cut(s) 544, 1108, 1348, 1448, 1517
BseSI GKGCMC 2 cut(s) 560, 978
BseX3I CGGCCG 1 cut(s) 534
BseXI GCAGC 6 cut(s) 186, 213, 468, 481, 1476, 1575
Bsh1285I CGRYCG 1 cut(s) 537
BshFI GGCC 6 cut(s) 323, 380, 536, 558, 576, 1193
BshNI GGYRCC 4 cut(s) 262, 286, 310, 343
BsiEI CGRYCG 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 978, 1053
BsiSI CCGG 7 cut(s) 320, 326, 377, 383, 533, 573, 1622
BslFI GGGAC 4 cut(s) 314, 371, 413, 1444
BslI CCNNNNNNNGG 4 cut(s) 307, 396, 1151, 1554
BsmAI GTCTC 5 cut(s) 255, 753, 994, 999, 1212
BsmBI CGTCTC 2 cut(s) 255, 999
BsmFI GGGAC 4 cut(s) 314, 371, 413, 1444
BsmI GAATGC 1 cut(s) 476
BsnI GGCC 6 cut(s) 323, 380, 536, 558, 576, 1193
Bso31I GGTCTC 1 cut(s) 753
Bsp120I GGGCCC 1 cut(s) 556
Bsp1286I GDGCHC 3 cut(s) 560, 978, 1053
Bsp143I GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
BspACI CCGC 5 cut(s) 195, 204, 498, 812, 1077
BspANI GGCC 6 cut(s) 323, 380, 536, 558, 576, 1193
BspHI TCATGA 1 cut(s) 1648
BspMAI CTGCAG 2 cut(s) 1376, 1466
BspMI ACCTGC 1 cut(s) 1379
BspPI GGATC 3 cut(s) 214, 1282, 1538
BspT107I GGYRCC 4 cut(s) 262, 286, 310, 343
BspTNI GGTCTC 1 cut(s) 753
BsrDI GCAATG 2 cut(s) 314, 371
BsrFI RCCGGY 3 cut(s) 319, 376, 1621
BsrI ACTGG 5 cut(s) 544, 1108, 1348, 1448, 1517
BssAI RCCGGY 3 cut(s) 319, 376, 1621
BssECI CCNNGG 9 cut(s) 324, 381, 395, 481, 734, 1145, 1188, 1392, 1426
BssMI GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
BssNAI GTATAC 1 cut(s) 1120
BssNI GRCGYC 2 cut(s) 263, 368
BssSI CACGAG 2 cut(s) 551, 1600
BssT1I CCWWGG 3 cut(s) 481, 734, 1188
Bst1107I GTATAC 1 cut(s) 1120
Bst2BI CACGAG 2 cut(s) 551, 1600
Bst2UI CCWGG 4 cut(s) 260, 284, 999, 1146
Bst4CI ACNGT 8 cut(s) 697, 904, 1007, 1088, 1139, 1213, 1247, 1441
Bst6I CTCTTC 2 cut(s) 85, 718
BstACI GRCGYC 2 cut(s) 263, 368
BstAPI GCANNNNNTGC 1 cut(s) 494
BstBAI YACGTR 1 cut(s) 1123
BstC8I GCNNGC 9 cut(s) 79, 321, 378, 731, 1075, 1462, 1556, 1619, 1623
BstDEI CTNAG 4 cut(s) 444, 678, 683, 875
BstDSI CCRYGG 1 cut(s) 1392
BstF5I GGATG 3 cut(s) 1053, 1112, 1308
BstH2I RGCGCY 2 cut(s) 266, 1400
BstHHI GCGC 2 cut(s) 265, 1399
BstKTI GATC 7 cut(s) 209, 409, 525, 717, 1277, 1546, 1648
BstMAI GTCTC 5 cut(s) 255, 753, 994, 999, 1212
BstMBI GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
BstMCI CGRYCG 1 cut(s) 537
BstNI CCWGG 4 cut(s) 260, 284, 999, 1146
BstNSI RCATGY 1 cut(s) 1540
BstSCI CCNGG 6 cut(s) 258, 282, 324, 381, 997, 1144
BstSFI CTRYAG 3 cut(s) 1031, 1372, 1462
BstSLI GKGCMC 2 cut(s) 560, 978
BstSNI TACGTA 1 cut(s) 1123
BstV1I GCAGC 6 cut(s) 186, 213, 468, 481, 1476, 1575
BstV2I GAAGAC 1 cut(s) 120
BstZ17I GTATAC 1 cut(s) 1120
BstZI CGGCCG 1 cut(s) 534
BsuRI GGCC 6 cut(s) 323, 380, 536, 558, 576, 1193
BtgI CCRYGG 1 cut(s) 1392
BtgZI GCGATG 1 cut(s) 975
BtrI CACGTC 1 cut(s) 1489
BtsCI GGATG 3 cut(s) 1053, 1112, 1308
BveI ACCTGC 1 cut(s) 1379
Cac8I GCNNGC 9 cut(s) 79, 321, 378, 731, 1075, 1462, 1556, 1619, 1623
CciI TCATGA 1 cut(s) 1648
CfoI GCGC 2 cut(s) 265, 1399
Cfr10I RCCGGY 3 cut(s) 319, 376, 1621
Cfr13I GGNCC 9 cut(s) 322, 328, 379, 385, 556, 557, 575, 1142, 1578
CseI GACGC 2 cut(s) 376, 1594
Csp6I GTAC 3 cut(s) 1124, 1451, 1520
CviAII CATG 9 cut(s) 34, 82, 489, 859, 1151, 1228, 1263, 1537, 1649
CviQI GTAC 3 cut(s) 1124, 1451, 1520
DdeI CTNAG 4 cut(s) 444, 678, 683, 875
DinI GGCGCC 1 cut(s) 264
DpnI GATC 7 cut(s) 208, 408, 524, 716, 1276, 1545, 1647
DpnII GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
DraIII CACNNNGTG 1 cut(s) 1132
EaeI YGGCCR 1 cut(s) 534
EagI CGGCCG 1 cut(s) 534
Eam1104I CTCTTC 2 cut(s) 85, 718
EarI CTCTTC 2 cut(s) 85, 718
EclXI CGGCCG 1 cut(s) 534
Eco105I TACGTA 1 cut(s) 1123
Eco130I CCWWGG 3 cut(s) 481, 734, 1188
Eco147I AGGCCT 1 cut(s) 1193
Eco24I GRGCYC 1 cut(s) 560
Eco31I GGTCTC 1 cut(s) 753
Eco47I GGWCC 4 cut(s) 328, 385, 1142, 1578
Eco52I CGGCCG 1 cut(s) 534
EcoO109I RGGNCCY 2 cut(s) 557, 1578
EcoRI GAATTC 2 cut(s) 671, 1036
EcoRII CCWGG 4 cut(s) 258, 282, 997, 1144
EcoT14I CCWWGG 3 cut(s) 481, 734, 1188
EcoT38I GRGCYC 1 cut(s) 560
EgeI GGCGCC 1 cut(s) 264
EheI GGCGCC 1 cut(s) 264
ErhI CCWWGG 3 cut(s) 481, 734, 1188
Esp3I CGTCTC 2 cut(s) 255, 999
FaeI CATG 9 cut(s) 37, 85, 492, 862, 1154, 1231, 1266, 1540, 1652
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 4 cut(s) 314, 371, 413, 1444
FatI CATG 9 cut(s) 33, 81, 488, 858, 1150, 1227, 1262, 1536, 1648
FauI CCCGC 1 cut(s) 819
FblI GTMKAC 2 cut(s) 1119, 1485
Fnu4HI GCNGC 7 cut(s) 175, 202, 457, 495, 498, 1465, 1589
FokI GGATG 3 cut(s) 1040, 1119, 1295
FriOI GRGCYC 1 cut(s) 560
Fsp4HI GCNGC 7 cut(s) 175, 202, 457, 495, 498, 1465, 1589
FspBI CTAG 1 cut(s) 41
GlaI GCGC 2 cut(s) 264, 1398
GluI GCNGC 7 cut(s) 175, 202, 457, 495, 498, 1465, 1589
HaeII RGCGCY 2 cut(s) 266, 1400
HaeIII GGCC 6 cut(s) 323, 380, 536, 558, 576, 1193
HapII CCGG 7 cut(s) 320, 326, 377, 383, 533, 573, 1622
HgaI GACGC 2 cut(s) 376, 1594
HhaI GCGC 2 cut(s) 265, 1399
Hin1I GRCGYC 2 cut(s) 263, 368
Hin1II CATG 9 cut(s) 37, 85, 492, 862, 1154, 1231, 1266, 1540, 1652
Hin6I GCGC 2 cut(s) 263, 1397
HinP1I GCGC 2 cut(s) 263, 1397
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 8 cut(s) 919, 966, 1024, 1155, 1254, 1293, 1407, 1422
HpaII CCGG 7 cut(s) 320, 326, 377, 383, 533, 573, 1622
HphI GGTGA 6 cut(s) 173, 461, 1013, 1019, 1201, 1402
Hpy166II GTNNAC 7 cut(s) 976, 1120, 1126, 1135, 1142, 1486, 1607
Hpy188I TCNGA 6 cut(s) 463, 634, 924, 1217, 1427, 1645
Hpy188III TCNNGA 4 cut(s) 916, 1258, 1404, 1649
Hpy8I GTNNAC 7 cut(s) 976, 1120, 1126, 1135, 1142, 1486, 1607
Hpy99I CGWCG 4 cut(s) 67, 407, 722, 1588
HpyAV CCTTC 6 cut(s) 58, 90, 334, 700, 1171, 1558
HpyCH4III ACNGT 8 cut(s) 697, 904, 1007, 1088, 1139, 1213, 1247, 1441
HpyCH4IV ACGT 4 cut(s) 897, 1122, 1131, 1488
HpyCH4V TGCA 6 cut(s) 81, 488, 976, 1231, 1374, 1464
HpyF3I CTNAG 4 cut(s) 444, 678, 683, 875
HpySE526I ACGT 4 cut(s) 897, 1122, 1131, 1488
Hsp92I GRCGYC 2 cut(s) 263, 368
Hsp92II CATG 9 cut(s) 37, 85, 492, 862, 1154, 1231, 1266, 1540, 1652
HspAI GCGC 2 cut(s) 263, 1397
KasI GGCGCC 1 cut(s) 262
KroI GCCGGC 3 cut(s) 319, 376, 1621
KroNI GCCGGC 3 cut(s) 321, 378, 1623
Kzo9I GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
LmnI GCTCC 4 cut(s) 250, 464, 631, 1067
Lsp1109I GCAGC 6 cut(s) 186, 213, 468, 481, 1476, 1575
LweI GCATC 5 cut(s) 524, 921, 1097, 1170, 1642
MaeI CTAG 1 cut(s) 41
MaeII ACGT 4 cut(s) 897, 1122, 1131, 1488
MaeIII GTNAC 6 cut(s) 179, 898, 904, 1001, 1489, 1658
MalI GATC 7 cut(s) 208, 408, 524, 716, 1276, 1545, 1647
MboI GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
MboII GAAGA 3 cut(s) 102, 120, 735
MhlI GDGCHC 3 cut(s) 560, 978, 1053
MluCI AATT 6 cut(s) 67, 671, 849, 1036, 1611, 1639
Mly113I GGCGCC 1 cut(s) 263
MlyI GAGTC 3 cut(s) 975, 1263, 1302
MroNI GCCGGC 3 cut(s) 319, 376, 1621
MseI TTAA 1 cut(s) 1574
MslI CAYNNNNRTG 5 cut(s) 32, 165, 1226, 1313, 1539
MspA1I CMGCKG 3 cut(s) 195, 204, 1510
MspI CCGG 7 cut(s) 320, 326, 377, 383, 533, 573, 1622
MspR9I CCNGG 6 cut(s) 260, 284, 326, 383, 999, 1146
Mva1269I GAATGC 1 cut(s) 476
MvaI CCWGG 4 cut(s) 260, 284, 999, 1146
NaeI GCCGGC 3 cut(s) 321, 378, 1623
NarI GGCGCC 1 cut(s) 263
NciI CCSGG 2 cut(s) 326, 383
NdeII GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
NgoMIV GCCGGC 3 cut(s) 319, 376, 1621
NlaIII CATG 9 cut(s) 37, 85, 492, 862, 1154, 1231, 1266, 1540, 1652
NmeAIII GCCGAG 1 cut(s) 420
NmuCI GTSAC 6 cut(s) 179, 898, 904, 1001, 1489, 1658
NspI RCATGY 1 cut(s) 1540
PagI TCATGA 1 cut(s) 1648
PaqCI CACCTGC 1 cut(s) 1379
PceI AGGCCT 1 cut(s) 1193
PciI ACATGT 1 cut(s) 1536
PcsI WCGNNNNNNNCGW 4 cut(s) 51, 534, 1128, 1464
PctI GAATGC 1 cut(s) 476
PdiI GCCGGC 3 cut(s) 321, 378, 1623
PfeI GAWTC 5 cut(s) 919, 1024, 1155, 1407, 1422
PflFI GACNNNGTC 1 cut(s) 1223
PflMI CCANNNNNTGG 1 cut(s) 1151
PkrI GCNGC 7 cut(s) 176, 203, 458, 496, 499, 1466, 1590
PleI GAGTC 3 cut(s) 974, 1262, 1301
PluTI GGCGCC 1 cut(s) 266
PpsI GAGTC 3 cut(s) 974, 1262, 1301
Ppu21I YACGTR 1 cut(s) 1123
PpuMI RGGWCCY 1 cut(s) 1578
PscI ACATGT 1 cut(s) 1536
PsiI TTATAA 1 cut(s) 894
Psp5II RGGWCCY 1 cut(s) 1578
Psp6I CCWGG 4 cut(s) 258, 282, 997, 1144
PspGI CCWGG 4 cut(s) 258, 282, 997, 1144
PspOMI GGGCCC 1 cut(s) 556
PspPI GGNCC 9 cut(s) 322, 328, 379, 385, 556, 557, 575, 1142, 1578
PspPPI RGGWCCY 1 cut(s) 1578
PstI CTGCAG 2 cut(s) 1376, 1466
PsyI GACNNNGTC 1 cut(s) 1223
PvuII CAGCTG 1 cut(s) 1510
RsaI GTAC 3 cut(s) 1125, 1452, 1521
RsaNI GTAC 3 cut(s) 1124, 1451, 1520
RseI CAYNNNNRTG 5 cut(s) 32, 165, 1226, 1313, 1539
SaqAI TTAA 1 cut(s) 1574
SatI GCNGC 7 cut(s) 175, 202, 457, 495, 498, 1465, 1589
Sau3AI GATC 7 cut(s) 206, 406, 522, 714, 1274, 1543, 1645
Sau96I GGNCC 9 cut(s) 322, 328, 379, 385, 556, 557, 575, 1142, 1578
SchI GAGTC 3 cut(s) 975, 1263, 1302
ScrFI CCNGG 6 cut(s) 260, 284, 326, 383, 999, 1146
SduI GDGCHC 3 cut(s) 560, 978, 1053
SfaNI GCATC 5 cut(s) 524, 921, 1097, 1170, 1642
SfcI CTRYAG 3 cut(s) 1031, 1372, 1462
SfoI GGCGCC 1 cut(s) 264
SinI GGWCC 4 cut(s) 328, 385, 1142, 1578
SmiMI CAYNNNNRTG 5 cut(s) 32, 165, 1226, 1313, 1539
SmlI CTYRAG 1 cut(s) 944
SmoI CTYRAG 1 cut(s) 944
SnaBI TACGTA 1 cut(s) 1123
Sse9I AATT 6 cut(s) 67, 671, 849, 1036, 1611, 1639
SseBI AGGCCT 1 cut(s) 1193
SsiI CCGC 5 cut(s) 195, 204, 498, 812, 1077
SspDI GGCGCC 1 cut(s) 262
SspMI CTAG 1 cut(s) 41
StuI AGGCCT 1 cut(s) 1193
StyD4I CCNGG 6 cut(s) 258, 282, 324, 381, 997, 1144
StyI CCWWGG 3 cut(s) 481, 734, 1188
TaaI ACNGT 8 cut(s) 697, 904, 1007, 1088, 1139, 1213, 1247, 1441
TaiI ACGT 4 cut(s) 900, 1125, 1134, 1491
TaqI TCGA 4 cut(s) 65, 72, 669, 717
TasI AATT 6 cut(s) 67, 671, 849, 1036, 1611, 1639
TatI WGTACW 2 cut(s) 1450, 1519
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 5 cut(s) 919, 1024, 1155, 1407, 1422
Tru1I TTAA 1 cut(s) 1574
Tru9I TTAA 1 cut(s) 1574
TseFI GTSAC 6 cut(s) 179, 898, 904, 1001, 1489, 1658
TseI GCWGC 6 cut(s) 174, 201, 456, 494, 1464, 1588
Tsp45I GTSAC 6 cut(s) 179, 898, 904, 1001, 1489, 1658
TspDTI ATGAA 5 cut(s) 22, 789, 875, 1256, 1335
TspGWI ACGGA 3 cut(s) 237, 735, 771
Tth111I GACNNNGTC 1 cut(s) 1223
Van91I CCANNNNNTGG 1 cut(s) 1151
VneI GTGCAC 1 cut(s) 974
VpaK11BI GGWCC 4 cut(s) 328, 385, 1142, 1578
XapI RAATTY 4 cut(s) 67, 671, 849, 1036
XceI RCATGY 1 cut(s) 1540
XmiI GTMKAC 2 cut(s) 1119, 1485
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.