RLG00000005907

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0014180
Physical Location & Seq
Reverse (-)
2687444 .. 2691494
4051 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000005907

Sequence Viewer

Length: 1605 bp
ATGAGTGCGTTTAGCTTCAGAAGCCTCACATTCATGCTTTTAGTGGCCTTCCTCGTTTGGTCGTCGAATTTCGAGGCTTGCATGGGCAGAAGAGGAAGGCATTGGAGGCACAGCAGAGTTGCCTCGTCTTCTCTTTCAAAGAAAAAAGTGAAAACCTCTCACGGCAGCAGCAGTCACCACCACAATGGTGGAAGTAGCGGATCAAAACCAAAGCCGAAACAAAAACCAAAACCAAAGGTTCCATCTCATAACCAGGCACCAACTCCATCTCACAACAAGGCACCAACTCCACCCCATAACAAGGCGCCATTGCCAGCTCCGGTCCCGAAGCCGAAAGATGACGATCAAGTTTACTCCTATAATGTGCTGGATTTCGGTGCCAAGGGCGATGGCAGTTCCGATGACACAAAGGCATTCCAAGCTGCATGGGCTGCCGCCTGTAAAGTTGAGGCATCAAAGATCATCGTTCCGGCTGATTACAAGTTCCTCGTGGGCCCCATCTCCTTCTCCGGCCCCTACTGCCAACCAAACATCTTATTTCAGCTTGATGGCACAATAGTAGCTCCATCAGACTCGAAATGGGGCAAAGGTCTGTTTCAATGGCTCGAATTCACTAAGCTAAGAGGAATAACAGTTCAGGGGAAGGGTGTGATCGACGGAAGAGGCTCGCCTTGGTGGCAAGACACTCCCTACGGAGACCCTTATGATGATGAACTAAAACTCATTATACCACTAAACAGCACACACCCGCCAATGATACCGATAAGGAATGAGCTAAATGGGAAAATTCCAGGCATAAAACCAACAGCACTGAGGTTTTATGGGAGTTTTAACGTGACCGTCACAGGCATAACAATTCAGAATAGCCCCCAATGCCATCTCAAGTTCGACAACTGCGATGGAGTCTTGGTGCACGATATGTCTGTCTCATCTCCCGGTGACAGTCCTAATACTGATGGGATTCACCTACAGAATTCCAAAGATGTGCTCATCCATAGCTCCACTCTTGCTTGCGGAGATGACTGTATTTCCATACAAACTGGATGCTCAAATGTCTACGTACACAATGTGAACTGTGGACCAGGGCATGGAATCAGCATCGGAAGTTTGGGGAAGGATAACACCAAGGCCTGTGTCTCAAACATCACCATCCGAGACATAGTCATGCACAATACAATGACTGGTGTGAGAGTCAAGACATGGCAGGGTGGATCAGGGTCTGTTCAGGGAGTCCTATTCTCAAACATCCAAGTGAATGAAGTTCAACTTCCTATTGTGATAGACCAGTTCTACTGTGACAAAAGCACCTGCAGAAATCAATCATCAGCTGTGGCGCTCTCAGGAATCACCTACGAGAGAATTCGAGGAACATACACAGTAAAACCGGTTCATTTTGCCTGCAGTGACAGCCTACCCTGCGTAGACGTGACACTAAACGGCATACAGCTAAAGCCAGTGCAAGAACGCTATCACATGTATGATCCCTTCTGCTGGCAGACTTTCGGGGAGTTGAGGACCCCGACGCTGCCACCAATTCCGTGTTTACAGATTGGTAAGCCACCGAACAACAAGGTCAATTCGGATCATGATGCGTGTGACCTTTAG

Protein Analysis

535

Amino Acids

58.22

Weight (kDa)

8.83

Isoelectric Point (pI)

41.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 149 - 228 9.3e-09 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 266 - 484 3e-68 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1316
Acc36I ACCTGC 1 cut(s) 1316
AccB1I GGYRCC 4 cut(s) 256, 280, 304, 377
AccB7I CCANNNNNTGG 1 cut(s) 1088
AccI GTMKAC 2 cut(s) 1056, 1422
AciI CCGC 4 cut(s) 198, 435, 749, 1014
AclWI GGATC 4 cut(s) 208, 1219, 1475, 1590
AcsI RAATTY 5 cut(s) 67, 608, 786, 973, 1359
AcyI GRCGYC 1 cut(s) 305
AdeI CACNNNGTG 1 cut(s) 1069
AfaI GTAC 1 cut(s) 1062
AfiI CCNNNNNNNGG 3 cut(s) 301, 1088, 1491
AflIII ACRYGT 1 cut(s) 1473
AgeI ACCGGT 1 cut(s) 1384
AgsI TTSAA 3 cut(s) 138, 599, 1265
AjiI CACGTC 1 cut(s) 1426
AjnI CCWGG 3 cut(s) 252, 790, 1081
AleI CACNNNNGTG 1 cut(s) 186
AloI GAACNNNNNNTCC 2 cut(s) 618, 650
Alw21I GWGCWC 2 cut(s) 915, 990
Alw26I GTCTC 4 cut(s) 690, 931, 1141, 1149
Alw44I GTGCAC 1 cut(s) 911
AlwI GGATC 4 cut(s) 208, 1219, 1475, 1590
AlwNI CAGNNNCTG 1 cut(s) 1220
AoxI GGCC 4 cut(s) 45, 493, 511, 1128
ApaI GGGCCC 1 cut(s) 497
ApaLI GTGCAC 1 cut(s) 911
ApeKI GCWGC 5 cut(s) 165, 168, 422, 431, 1525
ApoI RAATTY 5 cut(s) 67, 608, 786, 973, 1359
AsiGI ACCGGT 1 cut(s) 1384
AspLEI GCGC 2 cut(s) 307, 1336
AspS9I GGNCC 6 cut(s) 322, 493, 494, 512, 1079, 1515
AsuC2I CCSGG 1 cut(s) 936
AsuHPI GGTGA 5 cut(s) 167, 950, 956, 1138, 1339
AvaII GGWCC 3 cut(s) 322, 1079, 1515
BaeGI GKGCMC 2 cut(s) 497, 915
BanI GGYRCC 4 cut(s) 256, 280, 304, 377
BanII GRGCYC 1 cut(s) 497
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BauI CACGAG 1 cut(s) 488
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 915, 990
BbvI GCAGC 5 cut(s) 177, 180, 409, 418, 1512
BceAI ACGGC 2 cut(s) 178, 1453
BciT130I CCWGG 3 cut(s) 254, 792, 1083
BcnI CCSGG 1 cut(s) 936
BcoDI GTCTC 4 cut(s) 690, 931, 1141, 1149
BfmI CTRYAG 3 cut(s) 968, 1309, 1399
BfoI RGCGCY 2 cut(s) 308, 1337
BfuAI ACCTGC 1 cut(s) 1316
BglI GCCNNNNNGGC 1 cut(s) 676
BisI GCNGC 6 cut(s) 166, 169, 423, 432, 435, 1526
BlsI GCNGC 6 cut(s) 167, 170, 424, 433, 436, 1527
Bme1390I CCNGG 4 cut(s) 254, 792, 936, 1083
Bme18I GGWCC 3 cut(s) 322, 1079, 1515
BmgBI CACGTC 1 cut(s) 1426
BmgT120I GGNCC 6 cut(s) 322, 493, 494, 512, 1079, 1515
BmrFI CCNGG 4 cut(s) 254, 792, 936, 1083
BmsI GCATC 4 cut(s) 461, 1034, 1107, 1579
BpiI GAAGAC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 936
BsaAI YACGTR 1 cut(s) 1060
BsaBI GATNNNNATC 1 cut(s) 342
BsaHI GRCGYC 1 cut(s) 305
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 4 cut(s) 381, 671, 1082, 1125
BsaWI WCCGGW 2 cut(s) 319, 1384
BsaXI ACNNNNNCTCC 2 cut(s) 894, 924
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 1088, 1491
Bse118I RCCGGY 1 cut(s) 1384
Bse1I ACTGG 4 cut(s) 1045, 1186, 1285, 1454
Bse3DI GCAATG 1 cut(s) 308
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 3 cut(s) 254, 792, 1083
BseDI CCNNGG 4 cut(s) 381, 671, 1082, 1125
BseGI GGATG 4 cut(s) 990, 1049, 1149, 1245
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 3 cut(s) 301, 1088, 1491
BseMI GCAATG 1 cut(s) 308
BseMII CTCAG 2 cut(s) 803, 1353
BseNI ACTGG 4 cut(s) 1045, 1186, 1285, 1454
BseSI GKGCMC 2 cut(s) 497, 915
BseXI GCAGC 5 cut(s) 177, 180, 409, 418, 1512
BshFI GGCC 4 cut(s) 47, 495, 513, 1130
BshNI GGYRCC 4 cut(s) 256, 280, 304, 377
BshTI ACCGGT 1 cut(s) 1384
BsiHKAI GWGCWC 2 cut(s) 915, 990
BsiSI CCGG 5 cut(s) 320, 470, 510, 936, 1385
BslFI GGGAC 1 cut(s) 308
BslI CCNNNNNNNGG 3 cut(s) 301, 1088, 1491
BsmAI GTCTC 4 cut(s) 690, 931, 1141, 1149
BsmFI GGGAC 1 cut(s) 308
BsmI GAATGC 1 cut(s) 413
BsnI GGCC 4 cut(s) 47, 495, 513, 1130
Bso31I GGTCTC 1 cut(s) 690
Bsp120I GGGCCC 1 cut(s) 493
Bsp1286I GDGCHC 3 cut(s) 497, 915, 990
Bsp143I GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
BspACI CCGC 4 cut(s) 198, 435, 749, 1014
BspANI GGCC 4 cut(s) 47, 495, 513, 1130
BspCNI CTCAG 2 cut(s) 804, 1352
BspHI TCATGA 1 cut(s) 1585
BspMAI CTGCAG 2 cut(s) 1313, 1403
BspMI ACCTGC 1 cut(s) 1316
BspPI GGATC 4 cut(s) 208, 1219, 1475, 1590
BspT107I GGYRCC 4 cut(s) 256, 280, 304, 377
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 1 cut(s) 308
BsrFI RCCGGY 1 cut(s) 1384
BsrI ACTGG 4 cut(s) 1045, 1186, 1285, 1454
BssAI RCCGGY 1 cut(s) 1384
BssECI CCNNGG 4 cut(s) 381, 671, 1082, 1125
BssMI GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
BssNI GRCGYC 1 cut(s) 305
BssSI CACGAG 1 cut(s) 488
BssT1I CCWWGG 3 cut(s) 381, 671, 1125
Bst2BI CACGAG 1 cut(s) 488
Bst2UI CCWGG 3 cut(s) 254, 792, 1083
Bst4CI ACNGT 7 cut(s) 634, 841, 944, 1025, 1076, 1295, 1378
Bst6I CTCTTC 2 cut(s) 85, 655
BstACI GRCGYC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 431
BstBAI YACGTR 1 cut(s) 1060
BstC8I GCNNGC 6 cut(s) 79, 315, 668, 1012, 1399, 1493
BstDEI CTNAG 4 cut(s) 615, 620, 812, 1339
BstF5I GGATG 4 cut(s) 990, 1049, 1149, 1245
BstH2I RGCGCY 2 cut(s) 308, 1337
BstHHI GCGC 2 cut(s) 307, 1336
BstKTI GATC 7 cut(s) 203, 346, 462, 654, 1214, 1483, 1585
BstMAI GTCTC 4 cut(s) 690, 931, 1141, 1149
BstMBI GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
BstNI CCWGG 3 cut(s) 254, 792, 1083
BstNSI RCATGY 1 cut(s) 1477
BstSCI CCNGG 4 cut(s) 252, 790, 934, 1081
BstSFI CTRYAG 3 cut(s) 968, 1309, 1399
BstSLI GKGCMC 2 cut(s) 497, 915
BstSNI TACGTA 1 cut(s) 1060
BstV1I GCAGC 5 cut(s) 177, 180, 409, 418, 1512
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 2 cut(s) 185, 188
BsuRI GGCC 4 cut(s) 47, 495, 513, 1130
BtgZI GCGATG 2 cut(s) 402, 912
BtrI CACGTC 1 cut(s) 1426
BtsCI GGATG 4 cut(s) 990, 1049, 1149, 1245
BtsI GCAGTG 1 cut(s) 1408
BtsIMutI CAGTG 3 cut(s) 809, 1408, 1461
BveI ACCTGC 1 cut(s) 1316
Cac8I GCNNGC 6 cut(s) 79, 315, 668, 1012, 1399, 1493
CaiI CAGNNNCTG 1 cut(s) 1220
CciI TCATGA 1 cut(s) 1585
CfoI GCGC 2 cut(s) 307, 1336
Cfr10I RCCGGY 1 cut(s) 1384
Cfr13I GGNCC 6 cut(s) 322, 493, 494, 512, 1079, 1515
CseI GACGC 1 cut(s) 1531
Csp6I GTAC 1 cut(s) 1061
CspAI ACCGGT 1 cut(s) 1384
CviAII CATG 8 cut(s) 34, 82, 426, 1088, 1165, 1200, 1474, 1586
CviQI GTAC 1 cut(s) 1061
DdeI CTNAG 4 cut(s) 615, 620, 812, 1339
DinI GGCGCC 1 cut(s) 306
DpnI GATC 7 cut(s) 202, 345, 461, 653, 1213, 1482, 1584
DpnII GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
DraIII CACNNNGTG 1 cut(s) 1069
Eam1104I CTCTTC 2 cut(s) 85, 655
EarI CTCTTC 2 cut(s) 85, 655
Eco105I TACGTA 1 cut(s) 1060
Eco130I CCWWGG 3 cut(s) 381, 671, 1125
Eco147I AGGCCT 1 cut(s) 1130
Eco24I GRGCYC 1 cut(s) 497
Eco31I GGTCTC 1 cut(s) 690
Eco47I GGWCC 3 cut(s) 322, 1079, 1515
EcoO109I RGGNCCY 2 cut(s) 494, 1515
EcoRI GAATTC 3 cut(s) 608, 973, 1359
EcoRII CCWGG 3 cut(s) 252, 790, 1081
EcoT14I CCWWGG 3 cut(s) 381, 671, 1125
EcoT38I GRGCYC 1 cut(s) 497
EgeI GGCGCC 1 cut(s) 306
EheI GGCGCC 1 cut(s) 306
ErhI CCWWGG 3 cut(s) 381, 671, 1125
FaeI CATG 8 cut(s) 37, 85, 429, 1091, 1168, 1203, 1477, 1589
FalI AAGNNNNNCTT 2 cut(s) 1251, 1283
FaqI GGGAC 1 cut(s) 308
FatI CATG 8 cut(s) 33, 81, 425, 1087, 1164, 1199, 1473, 1585
FauI CCCGC 1 cut(s) 756
FblI GTMKAC 2 cut(s) 1056, 1422
Fnu4HI GCNGC 6 cut(s) 166, 169, 423, 432, 435, 1526
FokI GGATG 4 cut(s) 977, 1056, 1136, 1232
FriOI GRGCYC 1 cut(s) 497
Fsp4HI GCNGC 6 cut(s) 166, 169, 423, 432, 435, 1526
GlaI GCGC 2 cut(s) 306, 1335
GluI GCNGC 6 cut(s) 166, 169, 423, 432, 435, 1526
HaeII RGCGCY 2 cut(s) 308, 1337
HaeIII GGCC 4 cut(s) 47, 495, 513, 1130
HapII CCGG 5 cut(s) 320, 470, 510, 936, 1385
HgaI GACGC 1 cut(s) 1531
HhaI GCGC 2 cut(s) 307, 1336
Hin1I GRCGYC 1 cut(s) 305
Hin1II CATG 8 cut(s) 37, 85, 429, 1091, 1168, 1203, 1477, 1589
Hin6I GCGC 2 cut(s) 305, 1334
HinP1I GCGC 2 cut(s) 305, 1334
HinfI GANTC 7 cut(s) 572, 903, 961, 1092, 1191, 1230, 1344
HpaII CCGG 5 cut(s) 320, 470, 510, 936, 1385
HphI GGTGA 5 cut(s) 167, 950, 956, 1138, 1339
Hpy166II GTNNAC 8 cut(s) 352, 913, 1057, 1063, 1072, 1079, 1423, 1544
Hpy188I TCNGA 7 cut(s) 20, 400, 571, 861, 1103, 1154, 1582
Hpy188III TCNNGA 4 cut(s) 325, 1195, 1341, 1586
Hpy8I GTNNAC 8 cut(s) 352, 913, 1057, 1063, 1072, 1079, 1423, 1544
Hpy99I CGWCG 3 cut(s) 67, 659, 1525
HpyAV CCTTC 6 cut(s) 58, 90, 514, 637, 1108, 1495
HpyCH4III ACNGT 7 cut(s) 634, 841, 944, 1025, 1076, 1295, 1378
HpyCH4IV ACGT 3 cut(s) 834, 1059, 1425
HpyCH4V TGCA 7 cut(s) 81, 425, 913, 1168, 1311, 1401, 1459
HpyF3I CTNAG 4 cut(s) 615, 620, 812, 1339
HpySE526I ACGT 3 cut(s) 834, 1059, 1425
Hsp92I GRCGYC 1 cut(s) 305
Hsp92II CATG 8 cut(s) 37, 85, 429, 1091, 1168, 1203, 1477, 1589
HspAI GCGC 2 cut(s) 305, 1334
KasI GGCGCC 1 cut(s) 304
Kzo9I GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
LmnI GCTCC 3 cut(s) 322, 568, 1004
Lsp1109I GCAGC 5 cut(s) 177, 180, 409, 418, 1512
LweI GCATC 4 cut(s) 461, 1034, 1107, 1579
MaeII ACGT 3 cut(s) 834, 1059, 1425
MaeIII GTNAC 8 cut(s) 173, 835, 841, 938, 1295, 1403, 1426, 1595
MalI GATC 7 cut(s) 202, 345, 461, 653, 1213, 1482, 1584
MboI GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
MboII GAAGA 3 cut(s) 102, 120, 672
MhlI GDGCHC 3 cut(s) 497, 915, 990
MluCI AATT 8 cut(s) 67, 608, 786, 855, 973, 1359, 1533, 1576
Mly113I GGCGCC 1 cut(s) 305
MlyI GAGTC 4 cut(s) 566, 912, 1200, 1239
MseI TTAA 1 cut(s) 831
MslI CAYNNNNRTG 6 cut(s) 32, 183, 186, 1163, 1250, 1476
MspA1I CMGCKG 1 cut(s) 1328
MspI CCGG 5 cut(s) 320, 470, 510, 936, 1385
MspR9I CCNGG 4 cut(s) 254, 792, 936, 1083
Mva1269I GAATGC 1 cut(s) 413
MvaI CCWGG 3 cut(s) 254, 792, 1083
NarI GGCGCC 1 cut(s) 305
NciI CCSGG 1 cut(s) 936
NdeII GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
NlaIII CATG 8 cut(s) 37, 85, 429, 1091, 1168, 1203, 1477, 1589
NmuCI GTSAC 8 cut(s) 173, 835, 841, 938, 1295, 1403, 1426, 1595
NspI RCATGY 1 cut(s) 1477
OliI CACNNNNGTG 1 cut(s) 186
PagI TCATGA 1 cut(s) 1585
PaqCI CACCTGC 1 cut(s) 1316
PceI AGGCCT 1 cut(s) 1130
PciI ACATGT 1 cut(s) 1473
PcsI WCGNNNNNNNCGW 1 cut(s) 894
PctI GAATGC 1 cut(s) 413
PfeI GAWTC 3 cut(s) 961, 1092, 1344
PflFI GACNNNGTC 1 cut(s) 1160
PflMI CCANNNNNTGG 1 cut(s) 1088
PinAI ACCGGT 1 cut(s) 1384
PkrI GCNGC 6 cut(s) 167, 170, 424, 433, 436, 1527
PleI GAGTC 4 cut(s) 566, 911, 1199, 1238
PluTI GGCGCC 1 cut(s) 308
PpsI GAGTC 4 cut(s) 566, 911, 1199, 1238
Ppu21I YACGTR 1 cut(s) 1060
PpuMI RGGWCCY 1 cut(s) 1515
PscI ACATGT 1 cut(s) 1473
Psp5II RGGWCCY 1 cut(s) 1515
Psp6I CCWGG 3 cut(s) 252, 790, 1081
PspGI CCWGG 3 cut(s) 252, 790, 1081
PspOMI GGGCCC 1 cut(s) 493
PspPI GGNCC 6 cut(s) 322, 493, 494, 512, 1079, 1515
PspPPI RGGWCCY 1 cut(s) 1515
PsrI GAACNNNNNNTAC 2 cut(s) 1371, 1403
PstI CTGCAG 2 cut(s) 1313, 1403
PstNI CAGNNNCTG 1 cut(s) 1220
PsyI GACNNNGTC 1 cut(s) 1160
PvuII CAGCTG 1 cut(s) 1328
RsaI GTAC 1 cut(s) 1062
RsaNI GTAC 1 cut(s) 1061
RseI CAYNNNNRTG 6 cut(s) 32, 183, 186, 1163, 1250, 1476
SaqAI TTAA 1 cut(s) 831
SatI GCNGC 6 cut(s) 166, 169, 423, 432, 435, 1526
Sau3AI GATC 7 cut(s) 200, 343, 459, 651, 1211, 1480, 1582
Sau96I GGNCC 6 cut(s) 322, 493, 494, 512, 1079, 1515
SchI GAGTC 4 cut(s) 566, 912, 1200, 1239
ScrFI CCNGG 4 cut(s) 254, 792, 936, 1083
SduI GDGCHC 3 cut(s) 497, 915, 990
SfaNI GCATC 4 cut(s) 461, 1034, 1107, 1579
SfcI CTRYAG 3 cut(s) 968, 1309, 1399
SfoI GGCGCC 1 cut(s) 306
SinI GGWCC 3 cut(s) 322, 1079, 1515
SmiMI CAYNNNNRTG 6 cut(s) 32, 183, 186, 1163, 1250, 1476
SmlI CTYRAG 1 cut(s) 881
SmoI CTYRAG 1 cut(s) 881
SnaBI TACGTA 1 cut(s) 1060
Sse9I AATT 8 cut(s) 67, 608, 786, 855, 973, 1359, 1533, 1576
SseBI AGGCCT 1 cut(s) 1130
SsiI CCGC 4 cut(s) 198, 435, 749, 1014
SspDI GGCGCC 1 cut(s) 304
StuI AGGCCT 1 cut(s) 1130
StyD4I CCNGG 4 cut(s) 252, 790, 934, 1081
StyI CCWWGG 3 cut(s) 381, 671, 1125
TaaI ACNGT 7 cut(s) 634, 841, 944, 1025, 1076, 1295, 1378
TaiI ACGT 3 cut(s) 837, 1062, 1428
TaqI TCGA 7 cut(s) 65, 72, 575, 606, 654, 888, 1363
TasI AATT 8 cut(s) 67, 608, 786, 855, 973, 1359, 1533, 1576
TauI GCSGC 1 cut(s) 437
TfiI GAWTC 3 cut(s) 961, 1092, 1344
Tru1I TTAA 1 cut(s) 831
Tru9I TTAA 1 cut(s) 831
TscAI CASTG 3 cut(s) 816, 1408, 1461
TseFI GTSAC 8 cut(s) 173, 835, 841, 938, 1295, 1403, 1426, 1595
TseI GCWGC 5 cut(s) 165, 168, 422, 431, 1525
Tsp45I GTSAC 8 cut(s) 173, 835, 841, 938, 1295, 1403, 1426, 1595
TspDTI ATGAA 4 cut(s) 22, 726, 1272, 1379
TspGWI ACGGA 3 cut(s) 672, 708, 1527
TspRI CASTG 3 cut(s) 816, 1408, 1461
Tth111I GACNNNGTC 1 cut(s) 1160
Van91I CCANNNNNTGG 1 cut(s) 1088
VneI GTGCAC 1 cut(s) 911
VpaK11BI GGWCC 3 cut(s) 322, 1079, 1515
XapI RAATTY 5 cut(s) 67, 608, 786, 973, 1359
XceI RCATGY 1 cut(s) 1477
XcmI CCANNNNNNNNNTGG 1 cut(s) 185
XmiI GTMKAC 2 cut(s) 1056, 1422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.