Rh4AG405700

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0014180
Physical Location & Seq
Forward (+)
70738675 .. 70742859
4185 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG405700.1

Sequence Viewer

Length: 1593 bp
ATGAGTGCGTTTAGCTTCAGAAGCCTCACATTCATGCTTCTAGTCGCCTTCCTCGTTTGGTCGTCGAATTTCGAGGCTTGCATGGGCAGAAGAGGAAGGCATTGGAGGCACAGCAGAGTTGCCTCGTCTTCTCTTTCAAAGAAAAAAGGGAAAACCTCTCACGGCAGCAGCAGTCACCACCACAATGGCGGAAGTAGCGGATCAAAACCAAAGCCGAAACCGAAACCAAAACCAAAACCAAAACCAAAGGCTCCATCTCATAACCAGGCACCAACTCCACCCCATAACAAGGCACCATTGCCAGCTCCGGTCCCGAAGCCGAAAGATGACGATCAAGTTTACTCCTATAATGTGCTGGATTTCGGTGCCAAGGGAGATGGCAGTTCCGATGACACAAAGGCATTCCAAGCTGCATGGGCTGCCGCCTGTAAAGTTGAGGCATCAAAGATCATCGTTCCGGCCAATTACCAGTTCCTCGTGGGCCCCATCTCCTTCTCCGGCCCCTACTGCCAACCAAACATCTTATTTCAGCTTGATGGCACAATAGTAGCTCCATCAGACTCGAAATGGGGCAAAGGTCTGTTTCAATGGCTCGAATTCACGAAGCTAAGAGGAATAACAGTTCAGGGGAAGGGTGTGATCGACGGAAGAGGCTCGCCTTGGTGGCAAGACACTCCCTACGGAGACCCTTACGATGATGAACTAAAACTCATTATACCACTAAACAGCACACACCCGCCAATGATACCGATAAGGAATGAGCTAAATGGGAAAATTCCAGGCATAAAACCAACAGCACTGAGGTTTTATGGGAGTTTTAACGTGACCGTCACAGGCATAACAATTCAGAACAGCCCCCAATGCCATCTCAAGTTTGACAACTGCGATGGAGTCTTGGTGCACGATATGTCTGTCTCATCTCCCGGTGACAGTCCTAATACTGATGGGATTCACCTACAGAATTCCAAAGATGTGCTCATCCATAGCTCCACTCTTGCTTGCGGAGATGACTGTATTTCCATACAAACTGGATGCTCAAATGTCTACGTACACAATGTGAACTGTGGACCAGGGCATGGAATCAGCATCGGAAGTTTGGGGAAGGATAACACCAAGGCCTGTGTCTCAAACATCACCGTTCGAGACATAGTCATGCACAATACAATGACTGGTGTGAGAGTCAAGACATGGCAGGGTGGATCAGGGTCTGTTCAGGGAGTCCTATTCTCAAACATCCAAGTGAATGAAGTTCAACTTCCTATTGTGATAGACCAGTTCTACTGTGACAAAAGCACTTGCAGAAACCAATCATCAGCTGTGGCGCTCTCAGGAATCACCTACGAGAGAATTCGAGGAACATACACAGTAAAACCGGTACATTTTGCCTGCAGTGACAGCCTACCCTGCGTAGACGTGACACTAAACGGCGTACAGCTAAAGCCAGTGCAAGAACGCTATCACATGTATGATCCCTTCTGCTGGCAGACTTTCGGGGAGTTGAGGACCCCGACGCTGCCACCAATTCCGTGTTTACAGATTGGTAAGCCGCCGAACAACAAGGTCAATTCGGATCATGATGCGTGTGACCTTTAG

Protein Analysis

530

Amino Acids

57.71

Weight (kDa)

8.89

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 145 - 224 8.2e-09 Glycosyl hydrolases family 28
Glyco_hydro_28 PF00295 262 - 480 1.1e-68 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 268, 292, 365
AccB7I CCANNNNNTGG 1 cut(s) 1076
AccI GTMKAC 2 cut(s) 1044, 1410
AciI CCGC 6 cut(s) 189, 198, 423, 737, 1002, 1547
AclWI GGATC 4 cut(s) 208, 1207, 1463, 1578
AcoI YGGCCR 1 cut(s) 459
AcsI RAATTY 5 cut(s) 67, 596, 774, 961, 1347
AdeI CACNNNGTG 1 cut(s) 1057
AfaI GTAC 3 cut(s) 1050, 1377, 1431
AfiI CCNNNNNNNGG 3 cut(s) 289, 1076, 1479
AflIII ACRYGT 1 cut(s) 1461
AgeI ACCGGT 1 cut(s) 1372
AgsI TTSAA 3 cut(s) 138, 587, 1253
AjiI CACGTC 1 cut(s) 1414
AjnI CCWGG 3 cut(s) 264, 778, 1069
AjuI GAANNNNNNNTTGG 2 cut(s) 455, 487
AloI GAACNNNNNNTCC 2 cut(s) 606, 638
Alw21I GWGCWC 2 cut(s) 903, 978
Alw26I GTCTC 4 cut(s) 678, 919, 1129, 1137
Alw44I GTGCAC 1 cut(s) 899
AlwI GGATC 4 cut(s) 208, 1207, 1463, 1578
AlwNI CAGNNNCTG 1 cut(s) 1208
AoxI GGCC 4 cut(s) 459, 481, 499, 1116
ApaI GGGCCC 1 cut(s) 485
ApaLI GTGCAC 1 cut(s) 899
ApeKI GCWGC 5 cut(s) 165, 168, 410, 419, 1513
ApoI RAATTY 5 cut(s) 67, 596, 774, 961, 1347
AsiGI ACCGGT 1 cut(s) 1372
AspLEI GCGC 1 cut(s) 1324
AspS9I GGNCC 6 cut(s) 310, 481, 482, 500, 1067, 1503
AsuC2I CCSGG 1 cut(s) 924
AsuHPI GGTGA 5 cut(s) 167, 938, 944, 1126, 1327
AvaII GGWCC 3 cut(s) 310, 1067, 1503
BaeGI GKGCMC 2 cut(s) 485, 903
BanI GGYRCC 3 cut(s) 268, 292, 365
BanII GRGCYC 1 cut(s) 485
BarI GAAGNNNNNNTAC 2 cut(s) 1457, 1489
BauI CACGAG 1 cut(s) 476
BbsI GAAGAC 1 cut(s) 120
Bbv12I GWGCWC 2 cut(s) 903, 978
BbvI GCAGC 5 cut(s) 177, 180, 397, 406, 1500
BccI CCATC 8 cut(s) 262, 371, 494, 530, 562, 873, 881, 938
BceAI ACGGC 2 cut(s) 178, 1441
BciT130I CCWGG 3 cut(s) 266, 780, 1071
BcnI CCSGG 1 cut(s) 924
BcoDI GTCTC 4 cut(s) 678, 919, 1129, 1137
BfaI CTAG 1 cut(s) 41
BfmI CTRYAG 2 cut(s) 956, 1387
BfoI RGCGCY 1 cut(s) 1325
BglI GCCNNNNNGGC 1 cut(s) 664
BisI GCNGC 7 cut(s) 166, 169, 411, 420, 423, 1514, 1547
BlsI GCNGC 7 cut(s) 167, 170, 412, 421, 424, 1515, 1548
Bme1390I CCNGG 4 cut(s) 266, 780, 924, 1071
Bme18I GGWCC 3 cut(s) 310, 1067, 1503
BmgBI CACGTC 1 cut(s) 1414
BmgT120I GGNCC 6 cut(s) 310, 481, 482, 500, 1067, 1503
BmiI GGNNCC 9 cut(s) 252, 270, 294, 312, 367, 483, 484, 502, 1505
BmrFI CCNGG 4 cut(s) 266, 780, 924, 1071
BmsI GCATC 4 cut(s) 449, 1022, 1095, 1567
BpiI GAAGAC 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 854
BpuMI CCSGG 1 cut(s) 924
BsaAI YACGTR 1 cut(s) 1048
BsaBI GATNNNNATC 1 cut(s) 330
BsaI GGTCTC 1 cut(s) 678
BsaJI CCNNGG 4 cut(s) 369, 659, 1070, 1113
BsaWI WCCGGW 2 cut(s) 307, 1372
BsaXI ACNNNNNCTCC 2 cut(s) 882, 912
Bsc4I CCNNNNNNNGG 3 cut(s) 289, 1076, 1479
Bse118I RCCGGY 1 cut(s) 1372
Bse1I ACTGG 5 cut(s) 469, 1033, 1174, 1273, 1442
Bse3DI GCAATG 1 cut(s) 296
Bse8I GATNNNNATC 1 cut(s) 330
BseBI CCWGG 3 cut(s) 266, 780, 1071
BseDI CCNNGG 4 cut(s) 369, 659, 1070, 1113
BseGI GGATG 3 cut(s) 978, 1037, 1233
BseJI GATNNNNATC 1 cut(s) 330
BseLI CCNNNNNNNGG 3 cut(s) 289, 1076, 1479
BseMI GCAATG 1 cut(s) 296
BseMII CTCAG 2 cut(s) 791, 1341
BseNI ACTGG 5 cut(s) 469, 1033, 1174, 1273, 1442
BseSI GKGCMC 2 cut(s) 485, 903
BseXI GCAGC 5 cut(s) 177, 180, 397, 406, 1500
BshFI GGCC 4 cut(s) 461, 483, 501, 1118
BshNI GGYRCC 3 cut(s) 268, 292, 365
BshTI ACCGGT 1 cut(s) 1372
BsiHKAI GWGCWC 2 cut(s) 903, 978
BsiSI CCGG 5 cut(s) 308, 458, 498, 924, 1373
BslFI GGGAC 1 cut(s) 296
BslI CCNNNNNNNGG 3 cut(s) 289, 1076, 1479
BsmAI GTCTC 4 cut(s) 678, 919, 1129, 1137
BsmFI GGGAC 1 cut(s) 296
BsmI GAATGC 1 cut(s) 401
BsnI GGCC 4 cut(s) 461, 483, 501, 1118
Bso31I GGTCTC 1 cut(s) 678
Bsp120I GGGCCC 1 cut(s) 481
Bsp1286I GDGCHC 3 cut(s) 485, 903, 978
Bsp143I GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
BspACI CCGC 6 cut(s) 189, 198, 423, 737, 1002, 1547
BspANI GGCC 4 cut(s) 461, 483, 501, 1118
BspCNI CTCAG 2 cut(s) 792, 1340
BspHI TCATGA 1 cut(s) 1573
BspLI GGNNCC 9 cut(s) 252, 270, 294, 312, 367, 483, 484, 502, 1505
BspMAI CTGCAG 1 cut(s) 1391
BspPI GGATC 4 cut(s) 208, 1207, 1463, 1578
BspT107I GGYRCC 3 cut(s) 268, 292, 365
BspTNI GGTCTC 1 cut(s) 678
BsrDI GCAATG 1 cut(s) 296
BsrFI RCCGGY 1 cut(s) 1372
BsrI ACTGG 5 cut(s) 469, 1033, 1174, 1273, 1442
BssAI RCCGGY 1 cut(s) 1372
BssECI CCNNGG 4 cut(s) 369, 659, 1070, 1113
BssMI GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
BssSI CACGAG 1 cut(s) 476
BssT1I CCWWGG 3 cut(s) 369, 659, 1113
Bst2BI CACGAG 1 cut(s) 476
Bst2UI CCWGG 3 cut(s) 266, 780, 1071
Bst4CI ACNGT 8 cut(s) 622, 829, 932, 1013, 1064, 1138, 1283, 1366
Bst6I CTCTTC 2 cut(s) 85, 643
BstAPI GCANNNNNTGC 1 cut(s) 419
BstBAI YACGTR 1 cut(s) 1048
BstC8I GCNNGC 6 cut(s) 79, 303, 656, 1000, 1387, 1481
BstDEI CTNAG 3 cut(s) 608, 800, 1327
BstF5I GGATG 3 cut(s) 978, 1037, 1233
BstH2I RGCGCY 1 cut(s) 1325
BstHHI GCGC 1 cut(s) 1324
BstKTI GATC 7 cut(s) 203, 334, 450, 642, 1202, 1471, 1573
BstMAI GTCTC 4 cut(s) 678, 919, 1129, 1137
BstMBI GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
BstNI CCWGG 3 cut(s) 266, 780, 1071
BstNSI RCATGY 1 cut(s) 1465
BstSCI CCNGG 4 cut(s) 264, 778, 922, 1069
BstSFI CTRYAG 2 cut(s) 956, 1387
BstSLI GKGCMC 2 cut(s) 485, 903
BstSNI TACGTA 1 cut(s) 1048
BstV1I GCAGC 5 cut(s) 177, 180, 397, 406, 1500
BstV2I GAAGAC 1 cut(s) 120
BstXI CCANNNNNNTGG 1 cut(s) 185
BsuRI GGCC 4 cut(s) 461, 483, 501, 1118
BtgZI GCGATG 1 cut(s) 900
BtrI CACGTC 1 cut(s) 1414
BtsCI GGATG 3 cut(s) 978, 1037, 1233
BtsI GCAGTG 1 cut(s) 1396
BtsIMutI CAGTG 3 cut(s) 797, 1396, 1449
Cac8I GCNNGC 6 cut(s) 79, 303, 656, 1000, 1387, 1481
CaiI CAGNNNCTG 1 cut(s) 1208
CciI TCATGA 1 cut(s) 1573
CfoI GCGC 1 cut(s) 1324
Cfr10I RCCGGY 1 cut(s) 1372
Cfr13I GGNCC 6 cut(s) 310, 481, 482, 500, 1067, 1503
CseI GACGC 1 cut(s) 1519
Csp6I GTAC 3 cut(s) 1049, 1376, 1430
CspAI ACCGGT 1 cut(s) 1372
CviAII CATG 8 cut(s) 34, 82, 414, 1076, 1153, 1188, 1462, 1574
CviQI GTAC 3 cut(s) 1049, 1376, 1430
DdeI CTNAG 3 cut(s) 608, 800, 1327
DpnI GATC 7 cut(s) 202, 333, 449, 641, 1201, 1470, 1572
DpnII GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
DraIII CACNNNGTG 1 cut(s) 1057
EaeI YGGCCR 1 cut(s) 459
Eam1104I CTCTTC 2 cut(s) 85, 643
EarI CTCTTC 2 cut(s) 85, 643
EciI GGCGGA 1 cut(s) 204
Eco105I TACGTA 1 cut(s) 1048
Eco130I CCWWGG 3 cut(s) 369, 659, 1113
Eco147I AGGCCT 1 cut(s) 1118
Eco24I GRGCYC 1 cut(s) 485
Eco31I GGTCTC 1 cut(s) 678
Eco47I GGWCC 3 cut(s) 310, 1067, 1503
EcoO109I RGGNCCY 2 cut(s) 482, 1503
EcoRI GAATTC 3 cut(s) 596, 961, 1347
EcoRII CCWGG 3 cut(s) 264, 778, 1069
EcoT14I CCWWGG 3 cut(s) 369, 659, 1113
EcoT38I GRGCYC 1 cut(s) 485
ErhI CCWWGG 3 cut(s) 369, 659, 1113
FaeI CATG 8 cut(s) 37, 85, 417, 1079, 1156, 1191, 1465, 1577
FalI AAGNNNNNCTT 2 cut(s) 1239, 1271
FaqI GGGAC 1 cut(s) 296
FatI CATG 8 cut(s) 33, 81, 413, 1075, 1152, 1187, 1461, 1573
FauI CCCGC 1 cut(s) 744
FblI GTMKAC 2 cut(s) 1044, 1410
Fnu4HI GCNGC 7 cut(s) 166, 169, 411, 420, 423, 1514, 1547
FokI GGATG 3 cut(s) 965, 1044, 1220
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 7 cut(s) 166, 169, 411, 420, 423, 1514, 1547
FspBI CTAG 1 cut(s) 41
GlaI GCGC 1 cut(s) 1323
GluI GCNGC 7 cut(s) 166, 169, 411, 420, 423, 1514, 1547
HaeII RGCGCY 1 cut(s) 1325
HaeIII GGCC 4 cut(s) 461, 483, 501, 1118
HapII CCGG 5 cut(s) 308, 458, 498, 924, 1373
HgaI GACGC 1 cut(s) 1519
HhaI GCGC 1 cut(s) 1324
Hin1II CATG 8 cut(s) 37, 85, 417, 1079, 1156, 1191, 1465, 1577
Hin6I GCGC 1 cut(s) 1322
HinP1I GCGC 1 cut(s) 1322
HinfI GANTC 7 cut(s) 560, 891, 949, 1080, 1179, 1218, 1332
HpaII CCGG 5 cut(s) 308, 458, 498, 924, 1373
HphI GGTGA 5 cut(s) 167, 938, 944, 1126, 1327
Hpy166II GTNNAC 8 cut(s) 340, 901, 1045, 1051, 1060, 1067, 1411, 1532
Hpy188I TCNGA 6 cut(s) 20, 388, 559, 849, 1091, 1570
Hpy188III TCNNGA 6 cut(s) 313, 601, 1142, 1183, 1329, 1574
Hpy8I GTNNAC 8 cut(s) 340, 901, 1045, 1051, 1060, 1067, 1411, 1532
Hpy99I CGWCG 3 cut(s) 67, 647, 1513
HpyAV CCTTC 6 cut(s) 58, 90, 502, 625, 1096, 1483
HpyCH4III ACNGT 8 cut(s) 622, 829, 932, 1013, 1064, 1138, 1283, 1366
HpyCH4IV ACGT 3 cut(s) 822, 1047, 1413
HpyCH4V TGCA 7 cut(s) 81, 413, 901, 1156, 1299, 1389, 1447
HpyF3I CTNAG 3 cut(s) 608, 800, 1327
HpySE526I ACGT 3 cut(s) 822, 1047, 1413
Hsp92II CATG 8 cut(s) 37, 85, 417, 1079, 1156, 1191, 1465, 1577
HspAI GCGC 1 cut(s) 1322
Kzo9I GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
LmnI GCTCC 4 cut(s) 256, 310, 556, 992
Lsp1109I GCAGC 5 cut(s) 177, 180, 397, 406, 1500
LweI GCATC 4 cut(s) 449, 1022, 1095, 1567
MaeI CTAG 1 cut(s) 41
MaeII ACGT 3 cut(s) 822, 1047, 1413
MaeIII GTNAC 8 cut(s) 173, 823, 829, 926, 1283, 1391, 1414, 1583
MalI GATC 7 cut(s) 202, 333, 449, 641, 1201, 1470, 1572
MboI GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
MboII GAAGA 3 cut(s) 102, 120, 660
MhlI GDGCHC 3 cut(s) 485, 903, 978
MluCI AATT 9 cut(s) 67, 463, 596, 774, 843, 961, 1347, 1521, 1564
MlyI GAGTC 4 cut(s) 554, 900, 1188, 1227
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 5 cut(s) 32, 183, 1151, 1238, 1464
MspA1I CMGCKG 1 cut(s) 1316
MspI CCGG 5 cut(s) 308, 458, 498, 924, 1373
MspR9I CCNGG 4 cut(s) 266, 780, 924, 1071
Mva1269I GAATGC 1 cut(s) 401
MvaI CCWGG 3 cut(s) 266, 780, 1071
NciI CCSGG 1 cut(s) 924
NdeII GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
NlaIII CATG 8 cut(s) 37, 85, 417, 1079, 1156, 1191, 1465, 1577
NlaIV GGNNCC 9 cut(s) 252, 270, 294, 312, 367, 483, 484, 502, 1505
NmuCI GTSAC 8 cut(s) 173, 823, 829, 926, 1283, 1391, 1414, 1583
NspI RCATGY 1 cut(s) 1465
PagI TCATGA 1 cut(s) 1573
PceI AGGCCT 1 cut(s) 1118
PciI ACATGT 1 cut(s) 1461
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 1 cut(s) 401
PfeI GAWTC 3 cut(s) 949, 1080, 1332
PflFI GACNNNGTC 1 cut(s) 1148
PflMI CCANNNNNTGG 1 cut(s) 1076
PinAI ACCGGT 1 cut(s) 1372
PkrI GCNGC 7 cut(s) 167, 170, 412, 421, 424, 1515, 1548
PleI GAGTC 4 cut(s) 554, 899, 1187, 1226
PpsI GAGTC 4 cut(s) 554, 899, 1187, 1226
Ppu21I YACGTR 1 cut(s) 1048
PpuMI RGGWCCY 1 cut(s) 1503
PscI ACATGT 1 cut(s) 1461
Psp5II RGGWCCY 1 cut(s) 1503
Psp6I CCWGG 3 cut(s) 264, 778, 1069
PspGI CCWGG 3 cut(s) 264, 778, 1069
PspN4I GGNNCC 9 cut(s) 252, 270, 294, 312, 367, 483, 484, 502, 1505
PspOMI GGGCCC 1 cut(s) 481
PspPI GGNCC 6 cut(s) 310, 481, 482, 500, 1067, 1503
PspPPI RGGWCCY 1 cut(s) 1503
PstI CTGCAG 1 cut(s) 1391
PstNI CAGNNNCTG 1 cut(s) 1208
PsyI GACNNNGTC 1 cut(s) 1148
PvuII CAGCTG 1 cut(s) 1316
RsaI GTAC 3 cut(s) 1050, 1377, 1431
RsaNI GTAC 3 cut(s) 1049, 1376, 1430
RseI CAYNNNNRTG 5 cut(s) 32, 183, 1151, 1238, 1464
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 7 cut(s) 166, 169, 411, 420, 423, 1514, 1547
Sau3AI GATC 7 cut(s) 200, 331, 447, 639, 1199, 1468, 1570
Sau96I GGNCC 6 cut(s) 310, 481, 482, 500, 1067, 1503
SchI GAGTC 4 cut(s) 554, 900, 1188, 1227
ScrFI CCNGG 4 cut(s) 266, 780, 924, 1071
SduI GDGCHC 3 cut(s) 485, 903, 978
SfaNI GCATC 4 cut(s) 449, 1022, 1095, 1567
SfcI CTRYAG 2 cut(s) 956, 1387
SinI GGWCC 3 cut(s) 310, 1067, 1503
SmiMI CAYNNNNRTG 5 cut(s) 32, 183, 1151, 1238, 1464
SmlI CTYRAG 1 cut(s) 869
SmoI CTYRAG 1 cut(s) 869
SnaBI TACGTA 1 cut(s) 1048
Sse9I AATT 9 cut(s) 67, 463, 596, 774, 843, 961, 1347, 1521, 1564
SseBI AGGCCT 1 cut(s) 1118
SsiI CCGC 6 cut(s) 189, 198, 423, 737, 1002, 1547
SspMI CTAG 1 cut(s) 41
StuI AGGCCT 1 cut(s) 1118
StyD4I CCNGG 4 cut(s) 264, 778, 922, 1069
StyI CCWWGG 3 cut(s) 369, 659, 1113
TaaI ACNGT 8 cut(s) 622, 829, 932, 1013, 1064, 1138, 1283, 1366
TaiI ACGT 3 cut(s) 825, 1050, 1416
TaqI TCGA 7 cut(s) 65, 72, 563, 594, 642, 1141, 1351
TasI AATT 9 cut(s) 67, 463, 596, 774, 843, 961, 1347, 1521, 1564
TauI GCSGC 2 cut(s) 425, 1549
TfiI GAWTC 3 cut(s) 949, 1080, 1332
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TscAI CASTG 3 cut(s) 804, 1396, 1449
TseFI GTSAC 8 cut(s) 173, 823, 829, 926, 1283, 1391, 1414, 1583
TseI GCWGC 5 cut(s) 165, 168, 410, 419, 1513
Tsp45I GTSAC 8 cut(s) 173, 823, 829, 926, 1283, 1391, 1414, 1583
TspDTI ATGAA 3 cut(s) 22, 714, 1260
TspGWI ACGGA 3 cut(s) 660, 696, 1515
TspRI CASTG 3 cut(s) 804, 1396, 1449
Tth111I GACNNNGTC 1 cut(s) 1148
Van91I CCANNNNNTGG 1 cut(s) 1076
VneI GTGCAC 1 cut(s) 899
VpaK11BI GGWCC 3 cut(s) 310, 1067, 1503
XapI RAATTY 5 cut(s) 67, 596, 774, 961, 1347
XceI RCATGY 1 cut(s) 1465
XmiI GTMKAC 2 cut(s) 1044, 1410
XspI CTAG 1 cut(s) 41
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.