pycom16g08130

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0014180
Physical Location & Seq
Reverse (-)
5365858 .. 5369676
3819 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g08130.7

Sequence Viewer

Length: 1566 bp
ATGAGTGAGTTCAGTTTCAGAAGCATCGCATTCATGCTTGTACTTGCATTTCTGGTTTGGTCGTCAAATTTCGACGCTTGCATGGGGAGAAGAGGGCGACATTGGAGGCAGAACAGAGCATCCTATGCTTCTCTTTCAAAGAAAAAAGGAAAAAGTAGCCATGGCGGCGGCGGGGGCAGCCACCACCACAGTGTTAGTGGATCAAAACCTAAAAGGGCACCATCTCCACCTCAAAACAAGGCACCATTGCCACCTCAAGTTCCCAAGCCAATAGTTGACACTCCACCAAGTTCTCCACAGAAAGGTTCATCCACTTTTAATGTGCTAGATTTTGGTGCTATGGGTGACGGCGGTACCGACGACACCAAGGCATTCCAAGCTGCATGGGCCGCTGCTTGTAAAGTTGAGGCATCAAATATCATTGTTCCAGCCGGTTACCAGTTCCTCGTGGGTCCCATCTCATTCTCCGGCCCTTACTGCCAGAAAAACATCCTTTTCCAGCTGGATGGTACGATTGTAGCGCCGACGGACTCGGAATCATGGAGCAAAGGTCTGTTCCAATGGCTTGAATTTACAAAGCTAAATGGAATCACAGTACAGGGAAAGGGAGTGATTGATGGAAGAGGCTCATCTTGGTGGCAAGACACTCCTTATGGAGACCCTATAGATGATGATTTCAAACTCATTGTGCCACTAAACAGCTCAGTCCAACAACGCCCGCCAATGCCGATAAGAAGCGAGCTGAGCGGGAAAATGCCAAGCATCAAACCGACGGCATTGCGATTTTATGGGAGTTTTAATGTGACGGTCACAGGCATAACGATTCAGAATAGCCCCCAATGCCATCTCAAGTTCGACAACTGCCAGGGAGTTCTGGTCCATGATATGAGCGTCTCGTCCCCTGGGGACAGTCCCAACACAGATGGAATTCACCTCCAAAACTCCCAAAGAGTGCTCATCCACACCACCACTCTTGCTTGCGGAGATGACTGTATTTCCATACAAACTGGATGCACAGATGTATACGTACACAACGTGAACTGTGGACCAGGGCATGGAATCAGCATTGGAAGTTTAGGAAGGGACAACACGAGAGCATGTGTTTCAAACATTACCGTTCGAGACATCATTATGCACAACACAATGAATGGTGTCAGAGTCAAAACATGGCAGGGTGGATCAGGCTCTGTTCAGGGAGTGCTGTTCTCAAACATTCAAGTTTCTGAAGTTCAACTCCCTATTGTGATTGACCAATTCTACTGTGACAAAAGCACATGCAAAAACCAAACATCAGCTGTGGCTCTGTCGGGGATCACCTACGAGAGAATAAGAGGAACATACACCGTAAAACCAGTACACTTCGCCTGCAGTGACAGCCTACCTTGCGTAGACGTAACATTGAATGGCATAGACCTGAAGCCATTGCAAGAAAATTATCACATGTACGACCCCTTCTGCTGGCAGACGTTCGGGGAGTTGAGAACCCCGACAGTCCCACCCATTGGTTGTTTACAGCTTGGCAAGCCATCGAGCAACAAAGTTAACTCAGATCACGATGTATGTTGA

Protein Analysis

522

Amino Acids

56.46

Weight (kDa)

8.37

Isoelectric Point (pI)

46.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014180)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G10640 AT1G60590
fragaria_vesca FvH4_4g34120
malus_domestica MD13G1094100.v1.1 MD16G1095400.v1.1
prunus_persica Prupe.1G251200_v2.0.a1
pyrus_communis pycom13g08200 pycom16g08130
rosa_chinensis RchiOBHm_Chr4g0443631
rosa_laevigata RLG00000005907
rosa_multiflora Rmu_co8340541.1_g000001 Rmu_sc0001118.1_g000012
rosa_roxburghii Rroxscaffold_5G00384180
rosa_samantha Rh2CG614500 Rh4AG405700 Rh4BG416700
rosa_wichuraiana Rw4G034830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 353
AccB1I GGYRCC 3 cut(s) 217, 241, 353
AccB7I CCANNNNNTGG 2 cut(s) 1055, 1502
AccBSI CCGCTC 1 cut(s) 747
AccI GTMKAC 2 cut(s) 1023, 1389
AciI CCGC 8 cut(s) 165, 168, 171, 351, 390, 719, 747, 981
AclWI GGATC 3 cut(s) 208, 1186, 1319
AcsI RAATTY 3 cut(s) 67, 569, 927
AcuI CTGAAG 2 cut(s) 1245, 1436
AdeI CACNNNGTG 1 cut(s) 1036
AfaI GTAC 7 cut(s) 42, 355, 511, 597, 1029, 1356, 1445
AfiI CCNNNNNNNGG 4 cut(s) 302, 1055, 1458, 1502
AflIII ACRYGT 1 cut(s) 1440
AgsI TTSAA 7 cut(s) 138, 569, 679, 1107, 1217, 1232, 1402
AjnI CCWGG 3 cut(s) 864, 901, 1048
AleI CACNNNNGTG 1 cut(s) 189
AloI GAACNNNNNNTCC 2 cut(s) 104, 136
AluBI AGCT 7 cut(s) 380, 502, 580, 702, 742, 1295, 1516
AluI AGCT 7 cut(s) 380, 502, 580, 702, 742, 1295, 1516
Alw21I GWGCWC 1 cut(s) 957
Alw26I GTCTC 3 cut(s) 651, 898, 1116
AlwI GGATC 3 cut(s) 208, 1186, 1319
AlwNI CAGNNNCTG 1 cut(s) 1187
AoxI GGCC 2 cut(s) 387, 469
ApeKI GCWGC 3 cut(s) 177, 380, 392
ApoI RAATTY 3 cut(s) 67, 569, 927
Asp718I GGTACC 1 cut(s) 353
AspLEI GCGC 1 cut(s) 523
AspS9I GGNCC 5 cut(s) 387, 452, 470, 877, 1046
AsuHPI GGTGA 3 cut(s) 356, 923, 1306
AvaII GGWCC 3 cut(s) 452, 877, 1046
BaeGI GKGCMC 1 cut(s) 220
BaeI ACNNNNGTAYC 4 cut(s) 337, 370, 501, 534
BanI GGYRCC 3 cut(s) 217, 241, 353
BarI GAAGNNNNNNTAC 2 cut(s) 1436, 1468
BauI CACGAG 2 cut(s) 446, 1090
Bbv12I GWGCWC 1 cut(s) 957
BbvI GCAGC 3 cut(s) 189, 367, 379
BccI CCATC 7 cut(s) 229, 464, 500, 611, 852, 917, 1534
BceAI ACGGC 2 cut(s) 364, 789
BcgI CGANNNNNNTGC 2 cut(s) 760, 794
BciT130I CCWGG 3 cut(s) 866, 903, 1050
BcoDI GTCTC 3 cut(s) 651, 898, 1116
BfaI CTAG 1 cut(s) 326
BfmI CTRYAG 2 cut(s) 663, 1366
BfoI RGCGCY 1 cut(s) 524
BglI GCCNNNNNGGC 1 cut(s) 165
BisI GCNGC 6 cut(s) 166, 169, 178, 381, 390, 393
BlpI GCTNAGC 1 cut(s) 743
BlsI GCNGC 6 cut(s) 167, 170, 179, 382, 391, 394
Bme1390I CCNGG 3 cut(s) 866, 903, 1050
Bme18I GGWCC 3 cut(s) 452, 877, 1046
BmgT120I GGNCC 5 cut(s) 387, 452, 470, 877, 1046
BmiI GGNNCC 5 cut(s) 219, 243, 355, 453, 454
BmrFI CCNGG 3 cut(s) 866, 903, 1050
BmsI GCATC 5 cut(s) 33, 128, 419, 771, 1001
Bpu1102I GCTNAGC 1 cut(s) 743
BpuEI CTTGAG 2 cut(s) 240, 833
BsaAI YACGTR 1 cut(s) 1027
BsaI GGTCTC 1 cut(s) 651
BsaJI CCNNGG 6 cut(s) 160, 366, 865, 901, 902, 1049
BsaXI ACNNNNNCTCC 4 cut(s) 535, 565, 861, 891
Bsc4I CCNNNNNNNGG 4 cut(s) 302, 1055, 1458, 1502
Bse118I RCCGGY 1 cut(s) 431
Bse1I ACTGG 3 cut(s) 439, 1012, 1352
Bse3DI GCAATG 3 cut(s) 245, 776, 1421
BseBI CCWGG 3 cut(s) 866, 903, 1050
BseDI CCNNGG 6 cut(s) 160, 366, 865, 901, 902, 1049
BseGI GGATG 6 cut(s) 119, 308, 489, 511, 957, 1016
BseLI CCNNNNNNNGG 4 cut(s) 302, 1055, 1458, 1502
BseMI GCAATG 3 cut(s) 245, 776, 1421
BseMII CTCAG 3 cut(s) 717, 734, 1560
BseNI ACTGG 3 cut(s) 439, 1012, 1352
BseSI GKGCMC 1 cut(s) 220
BseXI GCAGC 3 cut(s) 189, 367, 379
BshFI GGCC 2 cut(s) 389, 471
BshNI GGYRCC 3 cut(s) 217, 241, 353
BsiHKAI GWGCWC 1 cut(s) 957
BsiSI CCGG 2 cut(s) 432, 468
BslFI GGGAC 6 cut(s) 438, 883, 897, 920, 1097, 1478
BslI CCNNNNNNNGG 4 cut(s) 302, 1055, 1458, 1502
BsmAI GTCTC 3 cut(s) 651, 898, 1116
BsmBI CGTCTC 1 cut(s) 898
BsmFI GGGAC 6 cut(s) 438, 883, 897, 920, 1097, 1478
BsmI GAATGC 2 cut(s) 29, 371
BsnI GGCC 2 cut(s) 389, 471
Bso31I GGTCTC 1 cut(s) 651
Bsp1286I GDGCHC 2 cut(s) 220, 957
Bsp143I GATC 4 cut(s) 200, 1178, 1311, 1549
Bsp1720I GCTNAGC 1 cut(s) 743
Bsp19I CCATGG 1 cut(s) 160
BspACI CCGC 8 cut(s) 165, 168, 171, 351, 390, 719, 747, 981
BspANI GGCC 2 cut(s) 389, 471
BspCNI CTCAG 3 cut(s) 716, 735, 1559
BspLI GGNNCC 5 cut(s) 219, 243, 355, 453, 454
BspMAI CTGCAG 1 cut(s) 1370
BspPI GGATC 3 cut(s) 208, 1186, 1319
BspT107I GGYRCC 3 cut(s) 217, 241, 353
BspTNI GGTCTC 1 cut(s) 651
BsrBI CCGCTC 1 cut(s) 747
BsrDI GCAATG 3 cut(s) 245, 776, 1421
BsrFI RCCGGY 1 cut(s) 431
BsrI ACTGG 3 cut(s) 439, 1012, 1352
BssAI RCCGGY 1 cut(s) 431
BssECI CCNNGG 6 cut(s) 160, 366, 865, 901, 902, 1049
BssMI GATC 4 cut(s) 200, 1178, 1311, 1549
BssNAI GTATAC 1 cut(s) 1024
BssSI CACGAG 2 cut(s) 446, 1090
BssT1I CCWWGG 2 cut(s) 160, 366
Bst1107I GTATAC 1 cut(s) 1024
Bst2BI CACGAG 2 cut(s) 446, 1090
Bst2UI CCWGG 3 cut(s) 866, 903, 1050
Bst6I CTCTTC 2 cut(s) 85, 616
BstAPI GCANNNNNTGC 1 cut(s) 125
BstBAI YACGTR 1 cut(s) 1027
BstC8I GCNNGC 7 cut(s) 79, 719, 740, 979, 1366, 1460, 1523
BstDEI CTNAG 3 cut(s) 703, 743, 1546
BstDSI CCRYGG 1 cut(s) 160
BstEII GGTNACC 1 cut(s) 434
BstF5I GGATG 6 cut(s) 119, 308, 489, 511, 957, 1016
BstH2I RGCGCY 1 cut(s) 524
BstHHI GCGC 1 cut(s) 523
BstKTI GATC 4 cut(s) 203, 1181, 1314, 1552
BstMAI GTCTC 3 cut(s) 651, 898, 1116
BstMBI GATC 4 cut(s) 200, 1178, 1311, 1549
BstNI CCWGG 3 cut(s) 866, 903, 1050
BstNSI RCATGY 3 cut(s) 1101, 1278, 1444
BstPI GGTNACC 1 cut(s) 434
BstSCI CCNGG 3 cut(s) 864, 901, 1048
BstSFI CTRYAG 2 cut(s) 663, 1366
BstSLI GKGCMC 1 cut(s) 220
BstSNI TACGTA 1 cut(s) 1027
BstV1I GCAGC 3 cut(s) 189, 367, 379
BstXI CCANNNNNNTGG 1 cut(s) 506
BstZ17I GTATAC 1 cut(s) 1024
BsuRI GGCC 2 cut(s) 389, 471
BtgI CCRYGG 1 cut(s) 160
BtgZI GCGATG 1 cut(s) 10
BtsCI GGATG 6 cut(s) 119, 308, 489, 511, 957, 1016
BtsI GCAGTG 1 cut(s) 1375
BtsIMutI CAGTG 2 cut(s) 196, 1375
Cac8I GCNNGC 7 cut(s) 79, 719, 740, 979, 1366, 1460, 1523
CaiI CAGNNNCTG 1 cut(s) 1187
CfoI GCGC 1 cut(s) 523
Cfr10I RCCGGY 1 cut(s) 431
Cfr13I GGNCC 5 cut(s) 387, 452, 470, 877, 1046
CseI GACGC 2 cut(s) 83, 880
Csp6I GTAC 7 cut(s) 41, 354, 510, 596, 1028, 1355, 1444
CspCI CAANNNNNGTGG 2 cut(s) 955, 990
CviQI GTAC 7 cut(s) 41, 354, 510, 596, 1028, 1355, 1444
DdeI CTNAG 3 cut(s) 703, 743, 1546
DpnI GATC 4 cut(s) 202, 1180, 1313, 1551
DpnII GATC 4 cut(s) 200, 1178, 1311, 1549
DraIII CACNNNGTG 1 cut(s) 1036
Eam1104I CTCTTC 2 cut(s) 85, 616
EarI CTCTTC 2 cut(s) 85, 616
Eco105I TACGTA 1 cut(s) 1027
Eco130I CCWWGG 2 cut(s) 160, 366
Eco31I GGTCTC 1 cut(s) 651
Eco47I GGWCC 3 cut(s) 452, 877, 1046
Eco57I CTGAAG 2 cut(s) 1245, 1436
Eco91I GGTNACC 1 cut(s) 434
EcoO109I RGGNCCY 1 cut(s) 452
EcoO65I GGTNACC 1 cut(s) 434
EcoRI GAATTC 1 cut(s) 927
EcoRII CCWGG 3 cut(s) 864, 901, 1048
EcoT14I CCWWGG 2 cut(s) 160, 366
ErhI CCWWGG 2 cut(s) 160, 366
Esp3I CGTCTC 1 cut(s) 898
FaqI GGGAC 6 cut(s) 438, 883, 897, 920, 1097, 1478
FauI CCCGC 3 cut(s) 164, 726, 740
FblI GTMKAC 2 cut(s) 1023, 1389
Fnu4HI GCNGC 6 cut(s) 166, 169, 178, 381, 390, 393
FokI GGATG 6 cut(s) 106, 295, 476, 518, 944, 1023
Fsp4HI GCNGC 6 cut(s) 166, 169, 178, 381, 390, 393
FspBI CTAG 1 cut(s) 326
GlaI GCGC 1 cut(s) 522
GluI GCNGC 6 cut(s) 166, 169, 178, 381, 390, 393
HaeII RGCGCY 1 cut(s) 524
HaeIII GGCC 2 cut(s) 389, 471
HapII CCGG 2 cut(s) 432, 468
HgaI GACGC 2 cut(s) 83, 880
HhaI GCGC 1 cut(s) 523
Hin6I GCGC 1 cut(s) 521
HinP1I GCGC 1 cut(s) 521
HincII GTYRAC 2 cut(s) 277, 1543
HindII GTYRAC 2 cut(s) 277, 1543
HinfI GANTC 6 cut(s) 530, 536, 588, 823, 1059, 1158
HpaI GTTAAC 1 cut(s) 1543
HpaII CCGG 2 cut(s) 432, 468
HphI GGTGA 3 cut(s) 356, 923, 1306
Hpy166II GTNNAC 9 cut(s) 277, 1024, 1030, 1039, 1046, 1357, 1390, 1511, 1543
Hpy188I TCNGA 6 cut(s) 20, 535, 828, 1157, 1225, 1549
Hpy188III TCNNGA 2 cut(s) 1121, 1553
Hpy8I GTNNAC 9 cut(s) 277, 1024, 1030, 1039, 1046, 1357, 1390, 1511, 1543
Hpy99I CGWCG 4 cut(s) 77, 362, 529, 775
HpyAV CCTTC 2 cut(s) 1074, 1462
HpyCH4IV ACGT 4 cut(s) 1026, 1035, 1392, 1466
HpyCH4V TGCA 8 cut(s) 47, 81, 383, 1014, 1135, 1278, 1368, 1426
HpyF3I CTNAG 3 cut(s) 703, 743, 1546
HpySE526I ACGT 4 cut(s) 1026, 1035, 1392, 1466
HspAI GCGC 1 cut(s) 521
KflI GGGWCCC 1 cut(s) 452
KpnI GGTACC 1 cut(s) 357
KspAI GTTAAC 1 cut(s) 1543
Kzo9I GATC 4 cut(s) 200, 1178, 1311, 1549
LmnI GCTCC 1 cut(s) 543
Lsp1109I GCAGC 3 cut(s) 189, 367, 379
LweI GCATC 5 cut(s) 33, 128, 419, 771, 1001
MaeI CTAG 1 cut(s) 326
MaeII ACGT 4 cut(s) 1026, 1035, 1392, 1466
MaeIII GTNAC 7 cut(s) 344, 434, 802, 808, 1262, 1370, 1393
MalI GATC 4 cut(s) 202, 1180, 1313, 1551
MbiI CCGCTC 1 cut(s) 747
MboI GATC 4 cut(s) 200, 1178, 1311, 1549
MboII GAAGA 2 cut(s) 102, 633
MhlI GDGCHC 2 cut(s) 220, 957
MluCI AATT 5 cut(s) 67, 569, 927, 1253, 1432
MlyI GAGTC 2 cut(s) 524, 1167
MmeI TCCRAC 1 cut(s) 733
MnlI CCTC 9 cut(s) 86, 99, 240, 264, 400, 455, 617, 944, 1325
MseI TTAA 3 cut(s) 318, 798, 1542
MslI CAYNNNNRTG 3 cut(s) 189, 634, 1130
MspA1I CMGCKG 3 cut(s) 392, 502, 1295
MspI CCGG 2 cut(s) 432, 468
MspR9I CCNGG 3 cut(s) 866, 903, 1050
Mva1269I GAATGC 2 cut(s) 29, 371
MvaI CCWGG 3 cut(s) 866, 903, 1050
NcoI CCATGG 1 cut(s) 160
NdeII GATC 4 cut(s) 200, 1178, 1311, 1549
NlaIV GGNNCC 5 cut(s) 219, 243, 355, 453, 454
NmuCI GTSAC 5 cut(s) 344, 802, 808, 1262, 1370
NspI RCATGY 3 cut(s) 1101, 1278, 1444
OliI CACNNNNGTG 1 cut(s) 189
PasI CCCWGGG 1 cut(s) 902
PciI ACATGT 1 cut(s) 1440
PcsI WCGNNNNNNNCGW 2 cut(s) 354, 1032
PctI GAATGC 2 cut(s) 29, 371
PfeI GAWTC 4 cut(s) 536, 588, 823, 1059
PflMI CCANNNNNTGG 2 cut(s) 1055, 1502
PkrI GCNGC 6 cut(s) 167, 170, 179, 382, 391, 394
PleI GAGTC 2 cut(s) 524, 1166
PpsI GAGTC 2 cut(s) 524, 1166
Ppu21I YACGTR 1 cut(s) 1027
PpuMI RGGWCCY 1 cut(s) 452
PscI ACATGT 1 cut(s) 1440
Psp5II RGGWCCY 1 cut(s) 452
Psp6I CCWGG 3 cut(s) 864, 901, 1048
PspEI GGTNACC 1 cut(s) 434
PspGI CCWGG 3 cut(s) 864, 901, 1048
PspN4I GGNNCC 5 cut(s) 219, 243, 355, 453, 454
PspPI GGNCC 5 cut(s) 387, 452, 470, 877, 1046
PspPPI RGGWCCY 1 cut(s) 452
PstI CTGCAG 1 cut(s) 1370
PstNI CAGNNNCTG 1 cut(s) 1187
PvuII CAGCTG 2 cut(s) 502, 1295
RsaI GTAC 7 cut(s) 42, 355, 511, 597, 1029, 1356, 1445
RsaNI GTAC 7 cut(s) 41, 354, 510, 596, 1028, 1355, 1444
RseI CAYNNNNRTG 3 cut(s) 189, 634, 1130
SaqAI TTAA 3 cut(s) 318, 798, 1542
SatI GCNGC 6 cut(s) 166, 169, 178, 381, 390, 393
Sau3AI GATC 4 cut(s) 200, 1178, 1311, 1549
Sau96I GGNCC 5 cut(s) 387, 452, 470, 877, 1046
SchI GAGTC 2 cut(s) 524, 1167
ScrFI CCNGG 3 cut(s) 866, 903, 1050
SduI GDGCHC 2 cut(s) 220, 957
SfaNI GCATC 5 cut(s) 33, 128, 419, 771, 1001
SfcI CTRYAG 2 cut(s) 663, 1366
SinI GGWCC 3 cut(s) 452, 877, 1046
SmiMI CAYNNNNRTG 3 cut(s) 189, 634, 1130
SmlI CTYRAG 2 cut(s) 255, 848
SmoI CTYRAG 2 cut(s) 255, 848
SnaBI TACGTA 1 cut(s) 1027
Sse9I AATT 5 cut(s) 67, 569, 927, 1253, 1432
SsiI CCGC 8 cut(s) 165, 168, 171, 351, 390, 719, 747, 981
SspMI CTAG 1 cut(s) 326
StyD4I CCNGG 3 cut(s) 864, 901, 1048
StyI CCWWGG 2 cut(s) 160, 366
TaiI ACGT 4 cut(s) 1029, 1038, 1395, 1469
TaqI TCGA 4 cut(s) 72, 855, 1120, 1529
TasI AATT 5 cut(s) 67, 569, 927, 1253, 1432
TatI WGTACW 3 cut(s) 40, 595, 1354
TauI GCSGC 3 cut(s) 168, 171, 392
TfiI GAWTC 4 cut(s) 536, 588, 823, 1059
Tru1I TTAA 3 cut(s) 318, 798, 1542
Tru9I TTAA 3 cut(s) 318, 798, 1542
TscAI CASTG 2 cut(s) 196, 1375
TseFI GTSAC 5 cut(s) 344, 802, 808, 1262, 1370
TseI GCWGC 3 cut(s) 177, 380, 392
Tsp45I GTSAC 5 cut(s) 344, 802, 808, 1262, 1370
TspDTI ATGAA 3 cut(s) 22, 297, 1160
TspGWI ACGGA 1 cut(s) 542
TspRI CASTG 2 cut(s) 196, 1375
Van91I CCANNNNNTGG 2 cut(s) 1055, 1502
VpaK11BI GGWCC 3 cut(s) 452, 877, 1046
XapI RAATTY 3 cut(s) 67, 569, 927
XceI RCATGY 3 cut(s) 1101, 1278, 1444
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmiI GTMKAC 2 cut(s) 1023, 1389
XspI CTAG 1 cut(s) 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.